Publications by authors named "Jin Cui"

230 Publications

Direct catalytic asymmetric and -selective vinylogous addition of butenolides to chromones.

Chem Sci 2020 Jun 22;11(27):7170-7176. Epub 2020 Jun 22.

Institute of Microbial Chemistry (BIKAKEN) Tokyo 141-0021 Japan

An -selective catalytic asymmetric Michael-type vinylogous addition of β,γ-butenolides to chromones was developed. The catalyst system developed herein is characterized by tuning of the steric and electronic effects using a proper Biphep-type chiral ligand to invert the diastereoselection, and improvement of the catalyst turnover by a coordinative phenolic additive. The catalytic protocol renders potentially biologically active natural product analogs accessible in good yield with moderate diastereoselectivity and high enantiomeric purity, mostly greater than 99% ee.
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http://dx.doi.org/10.1039/d0sc01914cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159409PMC
June 2020

Calcium Positively Mediates Blue Light-Induced Anthocyanin Accumulation in Hypocotyl of Soybean Sprouts.

Front Plant Sci 2021 28;12:662091. Epub 2021 May 28.

College of Life Sciences, Nanjing Agricultural University, Nanjing, China.

Soybean sprouts are a flavorful microgreen that can be eaten all year round and are widely favored in Southeast Asia. In this study, the regulatory mechanism of calcium on anthocyanin biosynthesis in soybean sprouts under blue light was investigated. The results showed that blue light, with a short wavelength, effectively induced anthocyanin accumulation in the hypocotyl of soybean sprout cultivar "Dongnong 690." Calcium supplementation further enhanced anthocyanin content, which was obviously inhibited by LaCl and neomycin treatment. Moreover, exogenous calcium changed the metabolism of anthocyanins, and seven anthocyanin compounds were detected. The trend of calcium fluorescence intensity in hypocotyl cells, as well as that of the inositol 1,4,5-trisphosphate and calmodulin content, was consistent with that of anthocyanins content. Specific spatial distribution patterns of calcium antimonate precipitation were observed in the ultrastructure of hypocotyl cells under different conditions. Furthermore, calcium application upregulated the expression of genes related to anthocyanin biosynthesis, and calcium inhibitors suppressed these genes. Finally, transcriptomics was performed to gain global insights into the molecular regulation mechanism of calcium-associated anthocyanin production. Genes from the flavonoid biosynthesis pathway were distinctly enriched among the differentially expressed genes, and weighted gene co-expression network analysis showed that two MYBs were related to the accumulation of anthocyanins. These results indicated that calcium released from apoplast and intracellular stores in specific spatial-temporal features promote blue light-induced anthocyanin accumulation by upregulation of the expression of genes related to anthocyanin synthesis of "Dongnong 690" hypocotyl. The findings deepen the understanding of the calcium regulation mechanism of blue light-induced anthocyanin accumulation in soybean sprouts, which will help growers produce high-quality foods beneficial for human health.
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http://dx.doi.org/10.3389/fpls.2021.662091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194075PMC
May 2021

RANKL from bone marrow adipose lineage cells promotes osteoclast formation and bone loss.

EMBO Rep 2021 Jun 13:e52481. Epub 2021 Jun 13.

Institute of Translational Medicine, Shanghai University, Shanghai, China.

Receptor activator of NF-κB ligand (RANKL) is essential for osteoclast formation and bone remodeling. Nevertheless, the cellular source of RANKL for osteoclastogenesis has not been fully uncovered. Different from peripheral adipose tissue, bone marrow (BM) adipose lineage cells originate from bone marrow mesenchymal stromal cells (BMSCs). Here, we demonstrate that adiponectin promoter-driven Cre expression (Adipoq ) can target bone marrow adipose lineage cells. We cross the Adipoq mice with rankl mice to conditionally delete RANKL from BM adipose lineage cells. Conditional deletion of RANKL increases cancellous bone mass of long bones in mice by reducing the formation of trabecular osteoclasts and inhibiting bone resorption but does not affect cortical bone thickness or resorption of calcified cartilage. Adipoq ; rankl mice exhibit resistance to estrogen deficiency and rosiglitazone (ROS)-induced trabecular bone loss but show bone loss induced by unloading. BM adipose lineage cells therefore represent an essential source of RANKL for the formation of trabecula osteoclasts and resorption of cancellous bone during remodeling under physiological and pathological conditions. Targeting bone marrow adiposity is a promising way of preventing pathological bone loss.
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http://dx.doi.org/10.15252/embr.202152481DOI Listing
June 2021

Structural comparison of the rostra of two species of weevils coexisting on Ailanthus altissima: the response to ecological demands of egg deposition.

BMC Ecol Evol 2021 05 28;21(1):101. Epub 2021 May 28.

Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing, 100083, China.

Background: Elongated rostra play an important role in the egg-laying of weevils, and its emergence plays a key role in the adaptive radiation of weevils. Eucryptorrhynchus scrobiculatus Motschulsky and E. brandti Harold co-occur on the same only host Ailanthus altissima, while their oviposition sites are different. In order to understand the adaptation between the rostra of the two weevils and their oviposition sites, the structural differentiation of the rostra in E. scrobiculatus and E. brandti was compared.

Results: The present study reveals that: (1) The rostra length of E. scrobiculatus and E. brandti was found to be correlated with body size, larger weevils have a correspondingly longer rostrum. The increase of rostra length may be a byproduct of larger weevils. (2) There were significant differences in the external shape of the two rostra, especially the shape of the mandibles of the mouthparts at the apex of the rostra used to excavate an oviposition cavity. (3) There was no difference in the size of the abductor muscles that control the extension of the mandibles, but there were significant differences in the size of the adductor muscles that control the contraction of the mandibles.

Conclusions: These structural differences reflect the functional potential ovipositional tactics of rostra, which is considered to be a response to the ecological demands of egg deposition, and also provide new insights into the coexistence of two weevil species in the same host A. altissima.
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http://dx.doi.org/10.1186/s12862-021-01824-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161989PMC
May 2021

Hypoxia-induced increase in GABA content is essential for restoration of membrane potential and preventing ROS-induced disturbance to ion homeostasis.

Plant Commun 2021 May 1;2(3):100188. Epub 2021 May 1.

International Research Centre for Environmental Membrane Biology, Foshan University, Foshan 528000, China.

When plants are exposed to hypoxic conditions, the level of γ-aminobutyric acid (GABA) in plant tissues increases by several orders of magnitude. The physiological rationale behind this elevation remains largely unanswered. By combining genetic and electrophysiological approach, in this work we show that hypoxia-induced increase in GABA content is essential for restoration of membrane potential and preventing ROS-induced disturbance to cytosolic K homeostasis and Ca signaling. We show that reduced O availability affects H-ATPase pumping activity, leading to membrane depolarization and K loss via outward-rectifying GORK channels. Hypoxia stress also results in HO accumulation in the cell that activates ROS-inducible Ca uptake channels and triggers self-amplifying "ROS-Ca hub," further exacerbating K loss via non-selective cation channels that results in the loss of the cell's viability. Hypoxia-induced elevation in the GABA level may restore membrane potential by pH-dependent regulation of H-ATPase and/or by generating more energy through the activation of the GABA shunt pathway and TCA cycle. Elevated GABA can also provide better control of the ROS-Ca hub by transcriptional control of RBOH genes thus preventing over-excessive HO accumulation. Finally, GABA can operate as a ligand directly controlling the open probability and conductance of K efflux GORK channels, thus enabling plants adaptation to hypoxic conditions.
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http://dx.doi.org/10.1016/j.xplc.2021.100188DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132176PMC
May 2021

Corrigendum: A Matrine Derivative M54 Suppresses Osteoclastogenesis and Prevents Ovariectomy-Induced Bone Loss by Targeting Ribosomal Protein S5.

Front Pharmacol 2021 29;12. Epub 2021 Mar 29.

Department of Orthopedics, Shanghai Changhai Hospital, Second Military Medical University, Shanghai, China.

[This corrects the article DOI: 10.3389/fphar.2018.00022.].
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http://dx.doi.org/10.3389/fphar.2021.605592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108601PMC
March 2021

The efficacy and safety of moxibustion for chronic fatigue syndrome: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 May;100(18):e25742

Guizhou University of Traditional Chinese Medicine, Guizhou.

Background: The pathogenesis of chronic fatigue syndrome (CFS) is not clear. The main purpose of treatment is to improve autoimmune function and relieve fatigue symptoms. Moxibustion is often used to treat diseases caused by low autoimmunity, especially in relieving fatigue symptoms. It is a superior therapy for CFS in traditional Chinese medicine. At present, there is a lack of the high level clinical evidence to support the moxibustion in the treatment of CFS, so this study will systematically review and analyze the currently available randomized controlled trials to evaluate the efficacy and safety of moxibustion in the treatment of CFS.

Methods: We will systematically search PubMed, EMBASE, Cochrane library, Sinomed, CNKI, VIP, and Wanfang Database, ClinicalTrials.gov and Chinese Clinical Trial Registry will also be searched. The time range for the search will be from database activation to March 31, 2021. The randomized controlled trials (RCTs) associated with moxibustion for CFS will be included, regardless of language.We will use the standard proposed in Cochrane Handbook 5.1.0 to assess the bias risk of a single RCT. The main outcome index of the study is Fatigue Assessment Instrument (FAI), secondary outcome indexes will include Fatigue Scale -14 (FS-14), Fatigue Severity Scale (FSS), Pittsburgh sleep quality index (PSQI), natural killer (NK) cells, interleukin- 2 (IL-2), T lymphocyte subsets (CD4+, CD8+), cure rate, total efficiency and adverse reactions. The random effect model meta was used to analyze the effect data of a single RCT. Heterogeneity will be measured by Cochran Q test and I-squared statistics. We will use 2 subgroup analyses to explore the source of heterogeneity. RCTs with high bias risk was excluded and adjustment effect model was used for sensitivity analysis to test the robustness of the meta-analysis results. The publication bias included in RCTs will be assessed by funnel plot and Egger test.

Results: This study will objectively and comprehensively evaluate the efficacy and safety of randomized controlled trials of moxibustion in the treatment of chronic fatigue syndrome, and the results will be submitted to peer-reviewed journals for publication.

Conclusion: This systematic review will provide clinicians with the latest high-quality evidence for the use of moxibustion in the treatment of chronic fatigue syndrome.

Inplasy Registration Number: INPLASY202140063.
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http://dx.doi.org/10.1097/MD.0000000000025742DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104202PMC
May 2021

A cellular and spatial map of the choroid plexus across brain ventricles and ages.

Cell 2021 May 30;184(11):3056-3074.e21. Epub 2021 Apr 30.

Department of Pathology, Boston Children's Hospital, Boston, MA 02115, USA; Graduate Program in Biophysics, Harvard University, Cambridge, MA 02115, USA. Electronic address:

The choroid plexus (ChP) in each brain ventricle produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier. Here, we construct a single-cell and spatial atlas of each ChP in the developing, adult, and aged mouse brain. We delineate diverse cell types, subtypes, cell states, and expression programs in epithelial and mesenchymal cells across ages and ventricles. In the developing ChP, we predict a common progenitor pool for epithelial and neuronal cells, validated by lineage tracing. Epithelial and fibroblast cells show regionalized expression by ventricle, starting at embryonic stages and persisting with age, with a dramatic transcriptional shift with maturation, and a smaller shift in each aged cell type. With aging, epithelial cells upregulate host-defense programs, and resident macrophages upregulate interleukin-1β (IL-1β) signaling genes. Our atlas reveals cellular diversity, architecture and signaling across ventricles during development, maturation, and aging of the ChP-brain barrier.
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http://dx.doi.org/10.1016/j.cell.2021.04.003DOI Listing
May 2021

Maize unstable factor for orange1 is essential for endosperm development and carbohydrate accumulation.

Plant Physiol 2021 Apr 26. Epub 2021 Apr 26.

Department of Plant Science, The Pennsylvania State University, University Park, PA 16802, USA.

Maize (Zea mays L.) Ufo1-1 is a spontaneous dominant mutation of the unstable factor for orange1 (ufo1). We recently cloned ufo1, which is a Poaceae specific gene expressed solely during seed development in maize. Here we have characterized Ufo1-1 and a loss-of-function Ds insertion allele (ufo1-Dsg) to decipher the role of ufo1 in maize. We found that both ufo1 mutant alleles impact sugars and hormones, and have defects in the basal endosperm transfer layer (BETL) and adjacent cell types. The Ufo1-1 BETL had reduced cell elongation and cell wall ingrowth, resulting in cuboidal shaped transfer cells. In contrast, the ufo1-Dsg BETL cells showed a reduced overall size with abnormal wall ingrowth. Expression analysis identified the impact of ufo1 on several genes essential for BETL development. The overexpression of Ufo1-1 in various tissues leads to ectopic phenotypes, including abnormal cell organization and stomata subsidiary cell defects. Interestingly, pericarp and leaf transcriptomes also showed that as compared to wild type, Ufo1-1 had ectopic expression of endosperm development-specific genes. This study shows that Ufo1-1 impacts the expression patterns of a wide range of genes involved in various developmental processes.
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http://dx.doi.org/10.1093/plphys/kiab183DOI Listing
April 2021

Zeolitic Imidazolate Framework-8/Polyaniline Nanocomposite Based Electrochemical Sensor for Sensitive Detection of Imidaclothiz.

Anal Sci 2021 Apr 23. Epub 2021 Apr 23.

College of Environmental Science and Engineering, Tongji University.

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http://dx.doi.org/10.2116/analsci.21P063DOI Listing
April 2021

Transmission analysis of COVID-19 with discrete time imported cases: Tianjin and Chongqing as cases.

Infect Dis Model 2021 31;6:618-631. Epub 2021 Mar 31.

Complex Systems Research Center, Shanxi University, Taiyuan, Shan'xi, 030006, China.

In 2020, an unexpectedly large outbreak of the coronavirus disease 2019 (COVID-19) epidemic was reported in mainland China. As we known, the epidemic was caused by imported cases in other provinces of China except for Hubei in 2020. In this paper, we developed a differential equation model with tracing isolation strategy with close contacts of newly confirmed cases and discrete time imported cases, to perform assessment and risk analysis for COVID-19 outbreaks in Tianjin and Chongqing city. Firstly, the model behavior without imported cases was given. Then, the real-time regeneration number in Tianjin and Chongqing city revealed a trend of rapidly rising, and then falling fast. Finally, sensitivity analysis demonstrates that the earlier with Wuhan lock-down, the fewer cases in these two cities. One can obtain that the tracing isolation of close contacts of newly confirmed cases could effectively control the spread of the disease. But it is not sensitive for the more contact tracing isolation days on confirmed cases, the fewer cases. Our investigation model could be potentially helpful to provide model building technology for the transmission of COVID-19.
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http://dx.doi.org/10.1016/j.idm.2021.03.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010348PMC
March 2021

[Clinical observation on distribution characteristics and rules of pain sensitivity points on body surface in patients with knee osteoarthritis].

Zhongguo Zhen Jiu 2020 Nov;40(11):1198-1201

College of Acupuncture-Moxibustion and Tuina, Chengdu University of TCM, Chengdu 610075, Sichuan Province, China.

Objective: To observe the distribution characteristics and rules of pain sensitivity points on body surface in patients with knee osteoarthritis (KOA).

Methods: A total of 916 patients with KOA were selected in this study, the pain sensitivity points of local site of knee joint were probed by thumb palpation. Tape was used to measure the distance between the pain sensitivity points and the most nearby acupoints. The Wagner tenderness measuring instrument was used to measure the tenderness threshold of pain sensitivity points.

Results: A total of 3618 pain sensitivity points were probed, among them, 3338 pain sensitivity points were sensitized. The minimum sensitization degree was 1.00, the maximum sensitization degree was 3.39, while the average sensitization degree was (2.16±0.60). Pain sensitivity points were distributed 0.37-1.73 around the acupoints, the average distance was (1.26±0.20) . Most of the pain sensitivity points (48.7%) were around Yingu (KI 10), Ququan (LR 8) and Xuehai (SP 10). The number and sensitization degree of pain sensitivity points were associated with McGill pain questionnaire score of patients with KOA (<0.001).

Conclusion: The pain sensitivity points of patients with KOA may be the expansion effect of acupoint areas in the disease states, pain sensitivity points are more likely to appear on the medial side of knee joint.
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http://dx.doi.org/10.13703/j.0255-2930.20190928-k0001DOI Listing
November 2020

A swine arterivirus deubiquitinase stabilizes two major envelope proteins and promotes production of viral progeny.

PLoS Pathog 2021 Mar 18;17(3):e1009403. Epub 2021 Mar 18.

Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States America.

Arteriviruses are enveloped positive-strand RNA viruses that assemble and egress using the host cell's exocytic pathway. In previous studies, we demonstrated that most arteriviruses use a unique -2 ribosomal frameshifting mechanism to produce a C-terminally modified variant of their nonstructural protein 2 (nsp2). Like full-length nsp2, the N-terminal domain of this frameshift product, nsp2TF, contains a papain-like protease (PLP2) that has deubiquitinating (DUB) activity, in addition to its role in proteolytic processing of replicase polyproteins. In cells infected with porcine reproductive and respiratory syndrome virus (PRRSV), nsp2TF localizes to compartments of the exocytic pathway, specifically endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and Golgi complex. Here, we show that nsp2TF interacts with the two major viral envelope proteins, the GP5 glycoprotein and membrane (M) protein, which drive the key process of arterivirus assembly and budding. The PRRSV GP5 and M proteins were found to be poly-ubiquitinated, both in an expression system and in cells infected with an nsp2TF-deficient mutant virus. In contrast, ubiquitinated GP5 and M proteins did not accumulate in cells infected with the wild-type, nsp2TF-expressing virus. Further analysis implicated the DUB activity of the nsp2TF PLP2 domain in deconjugation of ubiquitin from GP5/M proteins, thus antagonizing proteasomal degradation of these key viral structural proteins. Our findings suggest that nsp2TF is targeted to the exocytic pathway to reduce proteasome-driven turnover of GP5/M proteins, thus promoting the formation of GP5-M dimers that are critical for arterivirus assembly.
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http://dx.doi.org/10.1371/journal.ppat.1009403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971519PMC
March 2021

A new 2D Cu-MOF constructed from carboxylate ligands containing C-Hπ interactions as a recyclable responsive luminescent sensor for VOCs.

Dalton Trans 2021 Mar 17;50(12):4124-4128. Epub 2021 Mar 17.

Department of Chemistry, School of Sciences, Tianjin University, Tianjin 30035, P. R. China.

A new type of 2D metal-organic framework (MOF), namely Cu-MOF, was constructed from Cu(NO)·3HO and the novel ligand 1,4-bis(4-naphthoic acid)benzene. The C-Hπ interactions in the Cu-MOF effectively reduced aggregation-caused quenching (ACQ) due to restrictions in intramolecular motion (RIM), increased fluorescence, and controlled crystal packing properties, resulting in low dimensional parallelogram channels and a responsive "turn-on" fluorescence. The activated Cu-MOF exhibited excellent chemical sensing properties to various volatile organic compounds (VOCs) and showed excellent recyclability.
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http://dx.doi.org/10.1039/d0dt04363jDOI Listing
March 2021

Myostatin Promotes Osteoclastogenesis by Regulating Ccdc50 Gene Expression and RANKL-Induced NF-κB and MAPK Pathways.

Front Pharmacol 2020 26;11:565163. Epub 2020 Nov 26.

Department of Orthopedics, Shanghai Baoshan Luodian Hospital, Shanghai, China.

Myostatin is a crucial cytokine that is widely present in skeletal muscle and that negatively regulates the growth and development of muscle cells. Recent research has shown that myostatin might play an essential role in bone metabolism. In RAW264.7 cells and bone marrow monocytes (BMMCs), myostatin activates the expression of the II type receptor ActR II B. Here, we report that myostatin significantly promoted RANKL/M-CSF-induced osteoclastogenesis and activated NF-κB and MAPK pathways via the Ccdc50 gene. Overexpression of myostatin promoted osteoclastogenesis and osteoclastogenesis-related markers including c-Src, MMP9, CTR, CK, and NFATc1. Specifically, myostatin increased the phosphorylation of Smad2, which led to the activation of NF-κB and MAPK pathways to activate osteoclastogenesis. Ccdc50 was identified as a gene whose expression was highly decreased in osteoclastogenesis upon myostatin treatment, and it could inhibit the function of myostatin in osteoclastogenesis by blocking NF-κB and MAPKs pathways. Our study indicates that myostatin is a promising candidate target for inhibiting RANKL-mediated osteoclastogenesis and might participate in therapy for osteoporosis, and that the Ccdc50 gene plays a significant role in the regulatory process.
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http://dx.doi.org/10.3389/fphar.2020.565163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849192PMC
November 2020

Choroid plexus NKCC1 mediates cerebrospinal fluid clearance during mouse early postnatal development.

Nat Commun 2021 01 19;12(1):447. Epub 2021 Jan 19.

Department of Pathology, Boston Children's Hospital, Boston, MA, 02115, USA.

Cerebrospinal fluid (CSF) provides vital support for the brain. Abnormal CSF accumulation, such as hydrocephalus, can negatively affect perinatal neurodevelopment. The mechanisms regulating CSF clearance during the postnatal critical period are unclear. Here, we show that CSF K, accompanied by water, is cleared through the choroid plexus (ChP) during mouse early postnatal development. We report that, at this developmental stage, the ChP showed increased ATP production and increased expression of ATP-dependent K transporters, particularly the Na, K, Cl, and water cotransporter NKCC1. Overexpression of NKCC1 in the ChP resulted in increased CSF K clearance, increased cerebral compliance, and reduced circulating CSF in the brain without changes in intracranial pressure in mice. Moreover, ChP-specific NKCC1 overexpression in an obstructive hydrocephalus mouse model resulted in reduced ventriculomegaly. Collectively, our results implicate NKCC1 in regulating CSF K clearance through the ChP in the critical period during postnatal neurodevelopment in mice.
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http://dx.doi.org/10.1038/s41467-020-20666-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815709PMC
January 2021

[Exploration on effect mechanism of Miao medicinal acupuncture therapy in treatment of knee osteoarthritis based on TRPV ion channel].

Zhongguo Zhen Jiu 2020 Dec;40(12):1328-34

School of Acupuncture-Moxibustion and Tuina, Guizhou University of TCM, Guiyang 550025, China.

Objective: To observe the effect of Miao medicinal acupuncture therapy on transient receptor potential vanilloid (TRPV) channel in knee joint synovial tissue of the rabbits with knee osteoarthritis (KOA) model and to explore the mechanism of Miao medicinal acupuncture therapy in treatment of KOA.

Methods: Of 34 New Zealand male rabbits, 6 rabbits were selected randomly as the normal group. KOA model was established in the rest rabbits by injecting a mixture of papain and L-cysteine in right knee joints. The 24 successfully modeled rabbits were randomized into a model group, a Miao medicinal acupuncture therapy group, a dermal needle group and a smearing group, 6 rabbits in each one. In the Miao medicinal acupuncture therapy group, Miao medicinal acupuncture therapy was adopted, in which, the roller type of dermal needle was used on the surface of right knee joint [a rectangle shape formed by "Xuehai" (SP 10), "Liangqiu" (ST 34), "Yanglingquan" (GB 34) and "Yinlingquan" (SP 9)], rolling in a " shape, on which, Miao medicinal solution was smeared in advance. In the dermal needle group, the rolling stimulation was exerted on the right the right knee joint surface with the roller type of dermal needle. In the smearing group, Miao medicinal solution was smeared on the right knee joint surface. The intervention was given once every two days, 3 times weekly and the intervention was exerted consecutively for 4 weeks. Successively, on day 1, 21, 28, 35, 42 and 49 of experiment, paw withdrawal threshold (von Frey threshold) after mechanical stimulation was detected in the rabbits. HE staining was adopted to observe the histomorphological changes of the right knee joint cartilage in the rabbits. ELISA was used to determine the contents of interleukin-1 (IL-1β) and tumor necrosis factor-α (TNF-α) in the right knee synovial fluid. Western blot method and real-time PCR were used to determine the relative expressions of protein and mRNA of TRPV1 and TRPV4 in knee synovial tissue of the rabbits.

Results: Compared with the normal group, on day 49 of experiment, von Frey threshold was reduced significantly in the rabbits of the model group (<0.01), the integrety of cartilage surface of knee joint was seriously damaged, the structural layers were disordered, the chondrocytes were clustered, the tide lines were distorted and the matrix staining disappeared. The contents of IL-1β and TNF-α of the right knee synovial fluid were increased significantly (<0.01), and the relative expressions of protein and mRNA of TRPV1 and TRPV4 in the synovial tissue of the right knee were increased significantly (<0.01). Compared with the model group, on day 49 of experiment, von Frey threshold was increased significantly in each of the Miao medicinal acupuncture therapy group, the dermal needle group and the smearing group (<0.01). The right knee joint cartilage was complete in morphology, the structure clear was in layer, the cells were arranged in order and the matrix staining was uniform. The contents of IL-1β and TNF-α of the right knee synovial fluid were reduced significantly (<0.01, <0.05), and the relative expressions of protein and mRNA of TRPV1 and TRPV4 in the synovial tissue of the right knee were reduced significantly (<0.01). Compared with either the dermal needle group or the smearing group, on day 49 of experiment, von Frey threshold was increased significantly in the Miao medicinal acupuncture therapy group (<0.01). The right knee joint cartilage surface was even, the structure layers were clear. The contents of IL-1β and TNF-α of the right knee synovial fluid were reduced significantly (<0.05), and the relative expressions of protein of TRPV1 and TRPV4 in the synovial tissue were reduced (<0.01, <0.05). Compared with the smearing group, the relative expression of TRPV1 mRNA in the synovial tissue was reduced significantly in the Miao medicinal acupuncture therapy group (<0.05).

Conclusion: Miao medicinal acupuncture therapy plays a role in treatment of KOA probably through inhibiting the expressions of IL-1β and TNF-α of knee synovial fluid and down-regulating the expressions of protein and mRNA of TRPV1 and TRPV4 in knee synovial tissue.
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http://dx.doi.org/10.13703/j.0255-2930.20191111-k0002DOI Listing
December 2020

Enhanced vacuole compartmentalization of cadmium in root cells contributes to glutathione-induced reduction of cadmium translocation from roots to shoots in pakchoi (Brassica chinensis L.).

Ecotoxicol Environ Saf 2021 Jan 3;208:111616. Epub 2020 Dec 3.

College of Life Sciences, Nanjing Agricultural University, Nanjing, China. Electronic address:

Our previous studies showed that exogenous glutathione (GSH) decreased cadmium (Cd) concentration in shoots and alleviated the growth inhibition in pakchoi (Brassica chinensis L.) under Cd stress. Nevertheless, it is largely unknown how GSH decreases Cd accumulation in edible parts of pakchoi. This experiment mainly explored the mechanisms of GSH-induced reduction of Cd accumulation in shoot of pakchoi. The results showed that compared with sole Cd treatment, Cd + GSH treatment remarkably increased the expression of BcIRT1 and BcIRT2, and further enhanced the concentrations of Cd and Fe in root. By contrast, GSH application declined the concentration of Cd in the xylem sap. However, these results were not caused by xylem loading process because the expression of BcHMA2 and BcHMA4 had not significant difference between sole Cd treatment and Cd + GSH treatment. In addition, exogenous GSH significantly enhanced the expression of BcPCS1 and promoted the synthesis of PC, PC and PC under Cd stress. At the same time, exogenous GSH also significantly improved the expression of BcABCC1 and BcABCC2 in the roots of seedling under Cd stress, suggesting that more PCs-Cd complexes may be sequestrated into vacuoles by ABCC1 and ABCC2 transporters. The results showed that exogenous GSH could up-regulate the expression of BcIRT1/2 to increase the Cd accumulation in root, and the improvement of PCs contents and the expression of BcABCC1/2 enhanced the compartmentalization of Cd in root vacuole of pakchoi under Cd stress. To sum up, exogenous GSH reduce the concentration of free Cd in the cytoplast of root cells and then dropped the loading of Cd into the xylem, which eventually given rise to the reduction of Cd accumulation in edible portion of pakchoi.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111616DOI Listing
January 2021

IRT1 and ZIP2 were involved in exogenous hydrogen-rich water-reduced cadmium accumulation in Brassica chinensis and Arabidopsis thaliana.

J Hazard Mater 2021 04 13;407:124599. Epub 2020 Dec 13.

College of Life Sciences, Nanjing Agricultural University, Nanjing, China. Electronic address:

The results of Cd (cadmium) concentration, Cd fluorescent staining, NMT (non-invasive micro-test technology) analysis of Cd absorption revealed the remarkably positive role of HRW in reducing Cd uptake by root of pak choi seedlings. BcIRT1 (iron-regulated transporter 1) and BcZIP2 (zinc-regulated transporter protein 2) are the main Cd transporters in pak choi, but their roles in the process of HRW-reduced Cd uptake is still far from being answered. In this study, we specifically verified the function of IRT1 and ZIP2 in HRW-reduced Cd absorption in pak choi and Arabidopsis thaliana. Heterologous and homologous expression in Arabidopsis thaliana displayed that Cd concentrations in wild-type (Col-0) and transgenic A. thaliana of IRT1 and ZIP2 were significantly reduced by HRW, except for irt1- and zip2-mutant. NMT detection showed that HRW not only decreased Cd influx in root of WT and transgenic lines, but also enhanced the competition between Zn and Cd. Taken together, the HRW-induced reduction of Cd accumulation in plants may be result from depressing the expression of BcIRT1 and BcZIP2 and affecting the preference of BcIRT1 and BcZIP2 in ion uptake.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124599DOI Listing
April 2021

Long-Term Clinical Results of Use of an Anal Fistula Plug for Treatment of Low Trans-Sphincteric Anal Fistulas.

Med Sci Monit 2020 Dec 6;26:e928181. Epub 2020 Dec 6.

Department of General Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China (mainland).

BACKGROUND An anal fistula plug is a sphincter-sparing procedure that uses biological substances to close an anorectal fistula. This study aimed to evaluate the long-term therapeutic effect of an anal fistula plug procedure in patients with trans-sphincteric fistula-in-ano and to determine the risk factors affecting fistula healing. MATERIAL AND METHODS A single-center retrospective study was performed assessing long-term treatment outcomes of patients with low trans-sphincteric anal fistulas who initially underwent anal fistula plug procedures between August 2008 and September 2012. Risk factors affecting fistula healing were identified using univariate and multivariate analyses. RESULTS A total of 135 patients who had low trans-sphincteric anal fistulas and underwent anal fistula plug procedures were analysed. The overall healing rate was 56% (75/135) with a median follow-up time of 8 years (range, 72-121 months). The primary reasons for treatment failure were plug extrusion (n=12, 20%) and surgical site infection (n=9, 15%), occurring within 30 days after surgery. Multiple logistic regression analysis showed that the duration of anal fistula ≥6 months was significantly associated with treatment failure using an anal fistula plug (OR=3.187, 95% CI: 1.361-7.466, P=0.008). Of the patients who failed initial treatment with an anal fistula plug, 6 (9%) had anal fistulas that healed spontaneously after 2-3 years without additional treatment. CONCLUSIONS As a sphincter-preserving procedure, the anal fistula plug can effectively promote healing of low trans-sphincteric anal fistulas. The long-term efficacy is good and the procedure warrants wider use in clinical practice.
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http://dx.doi.org/10.12659/MSM.928181DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727077PMC
December 2020

Melatonin alleviates cadmium toxicity by reducing nitric oxide accumulation and IRT1 expression in Chinese cabbage seedlings.

Environ Sci Pollut Res Int 2021 Mar 25;28(12):15394-15405. Epub 2020 Nov 25.

College of Life Sciences, Nanjing Agricultural University, Nanjing, China.

Melatonin (MT) is reported as a kind of phytohormone, exerts various biological activities, mediating plant growth and development and responding to abiotic stresses. In the present research, we examined the possibility that MT could involve in the alleviation of cadmium (Cd) toxicity by reducing the accumulation of nitric oxide (NO). The research indicated that the addition of MT significantly increased the biomass and photosynthetic parameters of plants compared with the control treated under Cd stress. Besides, we found that compared with the control treatment, MT also reduced the level of Cd-induced nitric oxide, and at the same time, the enzyme activity related to NO synthesis and the expression of related genes were decreased. In addition, MT treatment significantly reduced the Cd content in Chinese cabbage seedlings compared with the control, which was partially reversed by the addition of SNP (NO donor). PTIO (NO scavenger) addition could reduce the Cd content when seedlings were exposed to Cd stress. At the same time, compared with the Cd stress, the concentration of Cd in MT-treated plants decreased significantly, and the expression levels of related transport genes IRT1 also decreased significantly. Taken together, these results further support the idea that under the stress of Cd, NO increases the expression of IRT1, thus further increasing the absorption of Cd and aggravating the stress of Cd in plants, while exogenously added MT can inhibit the synthesis of NO, reduce the content of Cd, and alleviate the stress caused by Cd.
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http://dx.doi.org/10.1007/s11356-020-11689-wDOI Listing
March 2021

Hemin-decreased cadmium uptake in pak choi (Brassica chinensis L.) seedlings is heme oxygenase-1 dependent and relies on its by-products ferrous iron and carbon monoxide.

Environ Pollut 2021 Apr 16;274:115882. Epub 2020 Oct 16.

College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

Cadmium (Cd) is a major pollutant in farmland, which not only greatly restricts crop production, but also brings a serious threat to human health through entering the food chain. Our previous study showed that hemin treatment could reduce the accumulation of Cd in pak choi seedlings. However, the underlying mechanism remains unclear. In this study, we used non-invasive micro-test technology (NMT) to detect the real-time Cd flux from pak choi roots and demonstrated that hemin treatment decreased Cd uptake rather than its translocation within plants. Moreover, through comparing the responses of different chemical treatments in pak choi seedlings and Arabidopsis wild-type and heme oxygenase-1 (HO-1) mutant, we provided evidence that hemin-decreased Cd uptake was HO-1 dependent. Furthermore, analyses of hemin degradation products suggested that the hemin-derived suppression of Cd uptake suppression was probably relying on its degradation by-products, ferrous iron (Fe) and carbon monoxide (CO), via repressing the expression of a Fe/Cd transporter BcIRT1 in pak choi roots.
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http://dx.doi.org/10.1016/j.envpol.2020.115882DOI Listing
April 2021

Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss.

Sci Adv 2020 Nov 18;6(47). Epub 2020 Nov 18.

Department of Orthopedics, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, China.

The actin-bundling protein L-plastin (LPL) mediates the resorption activity of osteoclasts, but its therapeutic potential in pathological bone loss remains unexplored. Here, we report that LPL knockout mice show increased bone mass and cortical thickness with more mononuclear tartrate-resistant acid phosphatase-positive cells, osteoblasts, CD31Emcn endothelial vessels, and fewer multinuclear osteoclasts in the bone marrow and periosteum. LPL deletion impeded preosteoclasts fusion by inhibiting filopodia formation and increased the number of preosteoclasts, which release platelet-derived growth factor-BB to promote CD31Emcn vessel growth and bone formation. LPL expression is regulated by the phosphatidylinositol 3-kinase/AKT/specific protein 1 axis in response to receptor activator of nuclear factor-κB ligand. Furthermore, we identified an LPL inhibitor, oroxylin A, that could maintain bone mass in ovariectomy-induced osteoporosis and accelerate bone fracture healing in mice. In conclusion, we showed that LPL regulates osteoclasts fusion, and targeting LPL serves as a novel anabolic therapy for pathological bone loss.
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http://dx.doi.org/10.1126/sciadv.abb7135DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673802PMC
November 2020

miR-25-3p promotes endothelial cell angiogenesis in aging mice via TULA-2/SYK/VEGFR-2 downregulation.

Aging (Albany NY) 2020 11 17;12(22):22599-22613. Epub 2020 Nov 17.

Division of Vascular Surgery, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.

In aging, the regulation of angiogenesis is a dynamic and complex process. We aimed to identify and characterize microRNAs that regulate angiogenesis during aging. We showed that, in response to vascular endothelial senescence, microRNA-25-3p (miR-25-3p) plays the role of an angiogenic microRNA by targeting TULA-2 (T-cell ubiquitin ligand-2)/SYK (spleen tyrosine kinase)/VEGFR-2 (vascular endothelial growth factor receptor 2) signaling and . Mechanistic studies demonstrated that miR-25-3p inhibits a TULA-2/SYK/VEGFR-2 signaling pathway in endothelial cells. In old endothelial cells (OECs), upregulation of miR-25-3p inhibited the expression of TULA-2, which caused downregulation of the interaction between TULA-2 and SYK and increased phosphorylation of SYK Y323. The increased SYK Y323 phosphorylation level upregulated the phosphorylation of VEGFR-2 Y1175, which plays a vital role in angiogenesis, while miR-25-3p downregulation in YECs showed opposite effects. Finally, a salvage study showed that miR-25-3p upregulation promoted capillary regeneration and hindlimb blood flow recovery in aging mice with hindlimb ischemia. These findings suggest that miR-25-3p acts as an agonist of TULA-2/SYK/VEGFR-2 and mediates the endothelial cell angiogenesis response, which shows that the miR-25-3p/TULA-2 pathway may be potential therapeutic targets for angiogenesis during aging.
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http://dx.doi.org/10.18632/aging.103834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746355PMC
November 2020

Comparison between osteosynthesis with interlocking nail and minimally invasive plating for proximal- and middle-thirds of humeral shaft fractures.

Int Orthop 2020 Nov 12. Epub 2020 Nov 12.

Department of Orthopedics, Shanghai Baoshan Luodian Hospital, Baoshan District, Shanghai, 201908, China.

Purpose: Options for the treatment of proximal- and middle-thirds of humeral shaft fractures include intramedullary interlocking nail (IMN) and minimally invasive plate osteosynthesis (MIPO). However, whether IMN provides better clinical outcomes than MIPO surgical technique still remains unclear. This study was designed to compare clinical outcomes of the IMN with MIPO technique for the treatment of proximal- and middle-thirds of humeral shaft fractures.

Method: A retrospective cohort analysis of 55 proximal- and middle-thirds of humeral shaft fractures surgically treated using IMN (n = 25) or MIPO (n = 30) from January 2012 to January 2016. Peri-operative and follow-up data (a minimum of 1 year) of the patients (aged from 18 to 56 years) were collected. Operative time, union time, VAS scores, surgery-related complications, and implant removal rate were compared between the two groups in this study. Besides, the functional outcomes were evaluated using the Rating Scale of American Shoulder and Elbow Surgeons' Form (ASES) and Mayo Elbow Performance Score System (MEPS).

Result: We found significantly shorter operative time and much less blood loss in IMN group, and lower VAS scores in the IMN group after surgery at first and third months but not at the sixth month. Complication rate was found to be relatively higher in the MIPO group when compared to the IMN group. No significant difference was observed between these two groups regarding ASES and MEPS scores. Three patients in the MIPO group suffered iatrogenic radial nerve injury and recovered after four to five months later. No implant failures occurred in either group.

Conclusion: Intramedullary interlocking nail seemed to be superior to minimally invasive plate osteosynthesis in the treatment of proximal- and middle-thirds of humeral shaft fractures due to shorter operative time and union time, less early post-operative pain, and fewer complications. The intramedullary interlocking nail could be considered a better surgical option for the management of proximal middle humeral fractures, though it may also depend on the surgeons' skills and learning curve. Further in-depth prospective studies are in great need to verify our conclusion.
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http://dx.doi.org/10.1007/s00264-020-04869-3DOI Listing
November 2020

Effects of an anal fistula plug on anal function after surgery for treatment of a trans-sphincteric anal fistula.

Langenbecks Arch Surg 2021 May 10;406(3):855-861. Epub 2020 Nov 10.

Department of General Surgery, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Gongtinan Road, Chaoyang District, Beijing, 100020, People's Republic of China.

Purpose: The aim of this study was to evaluate the anal function in patients with trans-sphincteric anal fistulas who underwent an anal fistula plug procedure, and analyze risk factors that might affect post-operative anal function.

Methods: This was an observational, retrospective study of patients diagnosed with trans-sphincteric anal fistulas and initially underwent anal fistula plug procedures between August 2008 and September 2012 at our institute. The analysis includes clinical characteristics, anal fistula healing, and the Wexner score for pre- and post-operative anal function (0 = no incontinence to 20 = complete incontinence).

Results: A total of 123 patients who had an adequate follow-up in the end were included. The median duration of follow-up was 8 years (range 72-121 months). The overall healing rate was 56% (69/123), and 33 (26.8%) patients had decreased anal function after surgery. The post-operative Wexner score on anal function was significantly higher than that before the operation (p < 0.001), as well as scores of gas, liquid stool, solid stool incontinence, and alteration in lifestyle (p < 0.05). Based on multiple logistic regression analysis, a high body mass index (p < 0.001) and long distance between the external opening and anal verge (p = 0.003) were significantly associated with a decline in post-operative anal function.

Conclusions: As a sphincter-preserving technique for the treatment of anal fistulas, the anal fistula plug procedure might impair the anal function. Especially for patients with obesity or long distance between the external opening of anal fistula and anal verge, the reduced anal function is more likely to occur after treatment with anal fistula plug therapy.
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http://dx.doi.org/10.1007/s00423-020-02024-5DOI Listing
May 2021

Antisense long non‑coding RNA WEE2‑AS1 regulates human vascular endothelial cell viability via cell cycle G2/M transition in arteriosclerosis obliterans.

Mol Med Rep 2020 Dec 22;22(6):5069-5082. Epub 2020 Oct 22.

Division of Vascular Surgery, The First Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China.

Long non‑coding RNAs (lncRNAs) affect atherosclerosis by regulating the physiological and pathological processes of endothelial cells; however, the role of lncRNA WEE2 antisense RNA 1 (WEE2‑AS1) in arteriosclerosis obliterans (ASO) is not completely understood. The present study aimed to explore the function of lncRNA WEE2‑AS1 in human vascular endothelial cells. The results indicated that lncRNA WEE2‑AS1 was significantly elevated in plasma and artery tissue samples of patients with ASO compared with healthy controls. The fluorescence in situ hybridization results suggested that lncRNA WEE2‑AS1 was expressed in the cytoplasm and nuclei of primary human umbilical vein endothelial cells (HUVECs). The Cell Counting Kit‑8 assay results suggested that lncRNA WEE2‑AS1 knockdown significantly promoted HUVEC viability, whereas lncRNA WEE2‑AS1 overexpression inhibited HUVEC viability compared with the negative control groups. Furthermore, analysis of the cell cycle by flow cytometry indicated that lncRNA WEE2‑AS1 knockdown significantly decreased the proportion of cells in the G0/G1 phase and significantly increased the proportion of cells in the G2/M phase compared with the negative control group. However, lncRNA WEE2‑AS1 overexpression had no significant effect on cell cycle distribution compared with the negative control group. The western blotting results indicated that lncRNA WEE2‑AS1 knockdown significantly reduced the expression levels of phosphorylated cyclin dependent kinase 1, WEE1 homolog 2 and myelin transcription factor 1, but increased the expression level of cell division cycle 25B compared with the negative control group. lncRNA WEE2‑AS1 overexpression displayed the opposite effect on protein expression. Collectively, the present study suggested that lncRNA WEE2‑AS1 was significantly upregulated in ASO and may serve a role in regulating human vascular endothelial cell viability. Further investigation into lncRNA WEE2‑AS1 may broaden the current understanding of the molecular mechanism underlying ASO, and aid with the identification of specific probes and precise targeted drugs for the diagnosis and treatment of ASO.
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http://dx.doi.org/10.3892/mmr.2020.11625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646961PMC
December 2020

A novel classification regression method for gridded electric power consumption estimation in China.

Sci Rep 2020 10 29;10(1):18558. Epub 2020 Oct 29.

College of Urban and Environment, Liaoning Normal University, Dalian, China.

Spatially explicit information on electric power consumption (EPC) is crucial for effective electricity allocation and utilization. Many studies have estimated fine-scale spatial EPC based on remotely sensed nighttime light (NTL). However, the spatial non-stationary relationship between EPC and NTL at prefectural level tends to be overlooked in existing literature. In this study, a classification regression method to estimate the gridded EPC in China based on imaging NTL via a Visible Infrared Imaging Radiometer Suite (VIIRS) was described. In addition, owing to some inherent omissions in the VIIRS NTL data, the study has employed the cubic Hermite interpolation to produce a more appropriate NTL dataset for estimation. The proposed method was compared with ordinary least squares (OLS) and geographically weighted regression (GWR) approaches. The results showed that our proposed method outperformed OLS and GWR in relative error (RE) and mean absolute percentage error (MAPE). The desirable results benefited mainly from a reasonable classification scheme that fully considered the spatial non-stationary relationship between EPC and NTL. Thus, the analysis suggested that the proposed classification regression method would enhance the accuracy of the gridded EPC estimation and provide a valuable reference predictive model for electricity consumption.
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http://dx.doi.org/10.1038/s41598-020-75543-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596565PMC
October 2020

Inflammation of the Embryonic Choroid Plexus Barrier following Maternal Immune Activation.

Dev Cell 2020 12 9;55(5):617-628.e6. Epub 2020 Oct 9.

Department of Pathology, Boston Children's Hospital, Boston, MA, USA; Graduate Program in Biophysics, Harvard University, Cambridge, MA, USA. Electronic address:

The choroid plexus (ChP) regulates brain development by secreting instructive cues and providing a protective brain barrier. Here, we show that polyI:C-mediated maternal immune activation leads to an inflammatory response in the developing embryonic mouse brain that manifests as pro-inflammatory cerebrospinal fluid (CSF) and accumulation of ChP macrophages. Elevation of CSF-CCL2 was sufficient to drive ChP immune cell recruitment, activation, and proliferation. In addition, ChP macrophages abandoned their regular tiling pattern and relocated to the ChP-free margin where they breached the weakened epithelial barrier. We further found that these immune cells entered from the ChP into the brain via anatomically specialized "hotspots" at the distal tips of ChP villi. In vivo two-photon imaging demonstrated that surveillance behaviors in ChP macrophages had already emerged at this early stage of embryogenesis. Thus, the embryonic ChP forms a functional brain barrier that can mount an inflammatory response to external insults.
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http://dx.doi.org/10.1016/j.devcel.2020.09.020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7725967PMC
December 2020