Publications by authors named "Jimin Kim"

106 Publications

Korean Society of Infectious Diseases/National Evidence-based Healthcare Collaborating Agency Recommendations for Anti-SARS-CoV-2 Monoclonal Antibody Treatment of Patients with COVID-19.

Infect Chemother 2021 Jun;53(2):395-403

Department of Internal Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.

Neutralizing antibodies targeted at the receptor-binding domain of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein have been developed and now under evaluation in clinical trials. The US Food and Drug Administration currently issued emergency use authorizations for neutralizing monoclonal antibodies in non-hospitalized patients with mild to moderate coronavirus disease 2019 (COVID-19) who are at high risk for progressing to severe disease and/or hospitalization. In terms of this situation, there is an urgent need to investigate the clinical aspects and to develop strategies to deploy them effectively in clinical practice. Here we provide guidance for the use of anti-SARS-CoV-2 monoclonal antibodies for the treatment of COVID-19 based on the latest evidence.
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http://dx.doi.org/10.3947/ic.2021.0304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258293PMC
June 2021

Proprioception, the regulator of motor function.

BMB Rep 2021 Jun 3. Epub 2021 Jun 3.

Department of Brain and Cognitive Sciences, DGIST, Daegu 42988; KBRI (Korea Brain Research Institute), Daegu 41068, Korea.

In animals, proper locomotion is crucial to find mates and foods and avoid predators or dangers. Multiple sensory systems detect external and internal cues and integrate them to modulate motor outputs. Proprioception is the internal sense of body position, and proprioceptive control of locomotion is essential to generate and maintain precise patterns of movement or gaits. This proprioceptive feedback system is conserved in many animal species and is mediated by stretch-sensitive receptors called proprioceptors. Recent studies have identified multiple proprioceptive neurons and proprioceptors and their roles in the locomotion of various model organisms. In this review we describe molecular and neuronal mechanisms underlying proprioceptive feedback systems in C. elegans, Drosophila, and mice.
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June 2021

Toward a Blueprint for Perioperative Handoffs and Handoff Tools.

Anesth Analg 2021 06;132(6):1559-1562

From the Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital, Boston, Massachusetts.

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http://dx.doi.org/10.1213/ANE.0000000000005514DOI Listing
June 2021

A Raf-like kinase is required for smoke-induced seed dormancy in .

Proc Natl Acad Sci U S A 2021 Apr;118(14)

Division of Integrative Bioscience and Biotechnology, Sejong University, 05006 Seoul, Republic of Korea;

Plants sense and integrate diverse stimuli to determine the timing for germination. A smoke compound, 3,4,5-trimethylfuran-2()-one (trimethylbutenolide, TMB), has been identified to inhibit the seed germination of higher plants. To understand the mode of action, we examined various physiological and molecular aspects of the TMB-dependent inhibition of seed germination in The results indicated that the effect of TMB is due to the enhanced physiological dormancy, which is modulated by other dormancy regulatory cues such as after-ripening, stratification, and ABA/GA signaling. In addition, gene expression profiling showed that TMB caused genome-wide transcriptional changes, altering the expression of a series of dormancy-related genes. Based on the TMB-responsive physiological contexts in , we performed mutant screening to isolate genetic components that underpin the TMB-induced seed dormancy. As a result, the () gene in , encoding a B2 group Raf-like kinase, was identified. Phenotypic analysis of the mutant implicated that has a critical role in the TMB-responsive gene expression and the inhibition of seed germination. Taken together, we propose that plants have been equipped with a TMB sensory pathway through which the TMB induces the seed dormancy in a TES1-dependent way.
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http://dx.doi.org/10.1073/pnas.2020636118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040645PMC
April 2021

Korean Christian Young Adults' Religiosity Affects Post-traumatic Growth: The Mediation Effects of Forgiveness and Gratitude.

J Relig Health 2021 Mar 7. Epub 2021 Mar 7.

Hankuk University of Foreign Studies, 107 Imunro, Dongdaemun-gu, Seoul, 130-791, South Korea.

This study examines the mediation effects of forgiveness and gratitude in the association between Korean Christian young adults' religiosity and post-traumatic growth. The participants are 296 Christian young adults in Korea. We hypothesize that the association between young Christian young adults' religiosity and post-traumatic growth is mediated by forgiveness and gratitude. The hypothesized model is tested by structural equation modeling. Results confirm that the religiosity of Christian young adults affects post-traumatic growth through forgiveness and gratitude. Adding a direct path from religiosity to post-traumatic growth significantly improved the model fit, which suggests partial mediation of forgiveness and gratitude in the association between religiosity and post-traumatic growth.
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http://dx.doi.org/10.1007/s10943-021-01213-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937049PMC
March 2021

Anti-Fatigue Activity of a Mixture of (Thunb.) Decaisne and Thunb. Fruit Extract.

Prev Nutr Food Sci 2020 Dec;25(4):380-388

Herbal Hormone Research Institute, Naturalendo Tech Co., Ltd., Gyeonggi 13486, Korea.

(Thunb.) Decaisne and Thunb. are commonly used in traditional herbal medicine and food and both exhibit antioxidant and anti-inflammatory effects. Herein, hot-water extracts of (Thunb.) Decaisne and Thunb. fruits (1:1 mixture) were used to produce a complex extract NET-1601. The anti-fatigue activity of NET-1601 was evaluated in an oxidative stress model induced by treating C2C12 myotubes with HO. An exhaustive swimming test (EST) model was established using ICR mice. NET-1601-treated C2C12 myotubes (50, 100, and 200 mg/mL) with HO-induced oxidative stress displayed significantly increased cell viability and ATP content, but significantly decreased levels of reactive oxygen species. All NET-1601-treated EST models demonstrated significantly higher maximum swimming rates than control mice. Furthermore, serum lactate, lactate dehydrogenase activity, non-esterified fatty acid, and intramuscular glycogen levels were higher in NET-1601-treated mice than in control mice. In addition, mRNA levels of regulatory factors involved in muscle mitochondrial fatty acid β-oxidation increased upon NET-1601 treatment. Moreover, catalase, superoxide dismutase, glutathione--transferase, and liver glutathione content, and antioxidant activity were higher in NET-1601-treated mice than in control mice. Reduced malondialdehyde levels indicated that NET-1601 treatment inhibited exercise-induced lipid peroxidation. Together, these results suggest that NET-1601 retains antioxidant enzyme activity during oxidative stress, simultaneously enhancing both muscle function via glycogen and fatty acid oxidation, thereby exerting a positive effect on recovery from fatigue.
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http://dx.doi.org/10.3746/pnf.2020.25.4.380DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813597PMC
December 2020

Gut microbiota and metabolic health among overweight and obese individuals.

Sci Rep 2020 11 10;10(1):19417. Epub 2020 Nov 10.

Center for Cohort Studies, Total Healthcare Center, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.

Although obesity is associated with numerous diseases, the risks of disease may depend on metabolic health. Associations between the gut microbiota, obesity, and metabolic syndrome have been reported, but differences in microbiomes according to metabolic health in the obese population have not been explored in previous studies. Here, we investigated the composition of gut microbiota according to metabolic health status in obese and overweight subjects. A total of 747 overweight or obese adults were categorized by metabolic health status, and their fecal microbiota were profiled using 16S ribosomal RNA gene sequencing. We classified these adults into a metabolically healthy group (MH, N = 317) without any components of metabolic syndrome or a metabolically unhealthy group (MU, N = 430) defined as having at least one metabolic abnormality. The phylogenetic and non-phylogenetic alpha diversity for gut microbiota were lower in the MU group than the MH group, and there were significant differences in gut microbiota bacterial composition between the two groups. We found that the genus Oscillospira and the family Coriobacteriaceae were associated with good metabolic health in the overweight and obese populations. This is the first report to describe gut microbial diversity and composition in metabolically healthy and unhealthy overweight and obese individuals. Modulation of the gut microbiome may help prevent metabolic abnormalities in the obese population.
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http://dx.doi.org/10.1038/s41598-020-76474-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7655835PMC
November 2020

Synthesis of (+)-Hypoxylactone through Allenoate γ-Addition: Revision of Stereochemistry.

J Org Chem 2020 11 28;85(21):14246-14252. Epub 2020 Oct 28.

Department of Chemistry, Chonnam National University, Gwangju 61186, Republic of Korea.

A synthesis of (+)-hypoxylactone has been accomplished in four steps starting from the allenoate γ-addition of -3-chloro-2-silyoxybutanals, leading to the revision of stereochemistry. The key was the discovery of control elements required to matching/mismatching cases in the allenoate γ-addition to provide the desired adducts as a single isomer. The utility of the γ-adduct was demonstrated with the Au(I)-catalyzed cyclization to afford (+)-xylogiblactone A. Use of AgO was the key to epoxidation for preventing epimerization of the γ-lactone ring.
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http://dx.doi.org/10.1021/acs.joc.0c02194DOI Listing
November 2020

Excess Maternal Deaths Associated With Coronavirus Disease 2019 (COVID-19) in Mexico.

Obstet Gynecol 2020 12;136(6):1114-1116

Departments of Obstetrics and Gynecology and Anesthesiology, Perioperative and Pain Medicine, Brigham & Women's Hospital, Harvard Medical School, and the Department of Postgraduate Medical Education, Harvard Medical School, Boston, Massachusetts; and Hospital Central "Dr. Ignacio Morones Prieto," San Luis Potosí, and Hospital General 450, Durango, Durango, Mexico.

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http://dx.doi.org/10.1097/AOG.0000000000004140DOI Listing
December 2020

Effective removal of radioactive cesium from contaminated water by synthesized composite adsorbent and its thermal treatment for enhanced storage stability.

Environ Res 2020 12 29;191:110099. Epub 2020 Aug 29.

Department of Environmental Engineering, Chungnam National University, 99, Daehak-ro, Yuseong-gu, Daejeon, 34134, Republic of Korea. Electronic address:

A composite adsorbent for the removal of radioactive cesium (Cs) was synthesized by immobilizing potassium cobalt ferrocyanide in the micro pores of the zeolite chabazite. The synthetically optimized composite adsorbent demonstrates a rapid cesium adsorption rate under both salt-free and high-salt conditions with a high distribution coefficient of cesium (≥10 mL/g). Although both components have the same ion-exchange reaction between potassium and cesium, the reaction by ferrocyanide component was predominant, which derived hundred times higher distribution coefficient of the composite adsorbent than that of pure chabazite. A thermal stabilization process was studied for improving the storage and/or disposal stability of the spent adsorbent. The formation of a eutectic system within the spent adsorbent reduced the stabilization temperature to 1000 °C from 1200 °C. Accordingly, the leaching of cesium was remarkably reduced by the remineralization to the stable pollucite. The stable impregnation of ferrocyanide component in the chabazite pores derived the reduction of cesium volatility enabling the high temperature stabilization method. Our experimental results provide evidence that the composite adsorbent has clear advantages on the cesium removal from contaminated water and its stabilization via thermal-treatment.
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http://dx.doi.org/10.1016/j.envres.2020.110099DOI Listing
December 2020

Major Lipids, Apolipoproteins, and Alterations of Gut Microbiota.

J Clin Med 2020 May 23;9(5). Epub 2020 May 23.

Center for Cohort Studies, Total Healthcare Center, Kangbuk Samsung Hospital, School of Medicine, Sungkyunkwan University, Seoul 04514, Korea.

The gut microbiota has been linked to blood lipids. However, the relationship between the gut microbiome and other lipid markers like apolipoproteins A1 (apoA1) and B (apoB) as well as classical lipid markers in Asians remain unclear. Here, we examined the associations between gut microbial diversity and taxonomic compositions with both apolipoproteins and lipid markers in a large number of Korean patients. The fecal 16S rRNA gene sequencing data from 1141 subjects were analyzed and subjects were categorized into control group (G0) or abnormal group (G1) according to blood lipid measurements. The microbial diversity and several taxa of the gut microbiota were significantly associated with triglyceride, apoA1, and apoB levels, but not with total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol levels. The alpha diversity of the gut microbiota was inversely associated with high triglyceride level. Interestingly, G1 of apoA1 showed increased microbial richness and distinct microbial community compared with G0 of apoA1. A high abundance of Fusobacteria and low abundance of were found in the hypertriglyceridemia group. In this large-scale study, we identified associations of gut microbiota with apolipoproteins and classical lipid markers, indicating that the gut microbiota may be an important target for regulating blood lipids.
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http://dx.doi.org/10.3390/jcm9051589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290464PMC
May 2020

Web-Based Dissemination of a Civic Engagement Curriculum to Promote Healthy Eating and Active Living in Rural Towns: The eHEART Study.

Int J Environ Res Public Health 2020 04 9;17(7). Epub 2020 Apr 9.

Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA.

Civic engagement interventions aimed at improving food and physical activity environments hold promise in addressing rural health disparities, but ensuring feasible and sustained dissemination remains a challenge. The present study aimed to evaluate the feasibility of a civic engagement curriculum adapted for online dissemination (Healthy Eating and Activity in Rural Towns (eHEART)). The eHEART curriculum and website were developed based on feedback from local health educators and community members. eHEART groups were facilitated by local Extension educators across three rural towns in three U.S. states (Montana, Wisconsin, and Alaska). Implementation feasibility was assessed through monthly project reports and interviews with educators. All eHEART groups successfully completed curriculum activities and met their project goals after nine months (November 2018 to July 2019). Groups ranged in size from 4 to 8 community residents and implemented varied strategies to improve aspects of their local food and/or physical activity environments. Facilitators of implementation included clear guidance on facilitating curriculum activities and the flexible and community-driven nature of eHEART projects. Recommended changes included more guidance on evaluating projects and contacting stakeholders as well as providing online tools and support for project management. Findings from this work have important implications for creating healthier rural environments. Local health educators and other community groups can feasibly use the eHEART curriculum to foster environmental changes that support healthy eating and active living.
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http://dx.doi.org/10.3390/ijerph17072571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177878PMC
April 2020

Depot-specific UCP1 expression in human white adipose tissue and its association with obesity-related markers.

Int J Obes (Lond) 2020 03 21;44(3):697-706. Epub 2020 Jan 21.

Department of General Surgery, College of Medicine, Inha University, Incheon, Republic of Korea.

Background: This study investigated depot-specific mRNA expression of uncoupling protein 1 (UCP1) in human white adipose tissue (WAT) and its association with obesity-related markers.

Methods: We recruited 39 normal-weight, 41 nondiabetic obese, and 22 diabetic obese women. We measured UCP1 mRNA expression in abdominal visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT), and investigated the associations between UCP1 mRNA expression in VAT and SAT, and obesity-related markers including mRNA expression of leptin, adiponectin, CCAAT-enhancer-binding protein homologous protein (CHOP), and positive regulatory domain-containing protein 16 (PRDM16). We also evaluated UCP1 mRNA expression in differentiated human white adipocytes after treatment with various stressors and metabolic improvement agents in vitro.

Results: UCP1 mRNA in VAT was significantly higher than in SAT in all groups. UCP1 mRNA in SAT was negatively correlated with BMI, total abdominal fat area, visceral fat area, blood pressure, fasting glucose, insulin, HOMA-IR score, triglyceride, hsCRP, fasting leptin levels, and adipocyte size. UCP1 mRNA in SAT was positively correlated with fasting adiponectin levels. UCP1 mRNA in VAT was negatively correlated with visceral-to-subcutaneous fat ratio (VSR), fasting glucose, and triglyceride levels. In SAT, UCP1 mRNA was negatively correlated with mRNA expression of leptin and CHOP, and positively correlated with mRNA expression of adiponectin. The expression of PRDM16 was positively correlated with UCP1 mRNA in both VAT and SAT. UCP1 mRNA expression in differentiated human white adipocytes was significantly reduced after incubation with thapsigargin, tunicamycin, homocysteine, TNF-α, or IL-β, and significantly increased after incubation with exendin 4, dapagliflozin, and telmisartan.

Conclusions: This study demonstrated depot-specific mRNA expression of UCP1 and its association with obesity-related markers in human WAT. UCP1 mRNA in human white adipocytes was suppressed by inflammatory agents and enhanced by metabolic improvement agents. UCP1 in human WAT might participate in the pathogenesis of obesity-related metabolic diseases.
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http://dx.doi.org/10.1038/s41366-020-0528-4DOI Listing
March 2020

Essential Amino Acid Supplement Lowers Intrahepatic Lipid despite Excess Alcohol Consumption.

Nutrients 2020 Jan 19;12(1). Epub 2020 Jan 19.

Department of Biology and Wildlife, University of Alaska Fairbanks, Fairbanks, AK 99775, USA.

Excess alcohol consumption is a top risk factor for death and disability. Fatty liver will likely develop and the risk of liver disease increases. We have previously demonstrated that an essential amino acid supplement (EAAS) improved protein synthesis and reduced intrahepatic lipid in the elderly. The purpose of this exploratory pilot study was to initiate the evaluation of EAAS on intrahepatic lipid (IHL), body composition, and blood lipids in individuals with mild to moderate alcohol use disorder (AUD). Following consent, determination of eligibility, and medical screening, 25 participants (18 males at 38 ± 15 years/age and 7 females at 34 ± 18 years/age) were enrolled and randomly assigned to one of two dosages: a low dose (LD: 8 g of EAAS twice/day (BID)) or high dose (HD: 13 g of EAAS BID). Five of the twenty-five enrolled participants dropped out of the intervention. Both groups consumed the supplement BID for 4 weeks. Pre- and post-EAAS administration, IHL was determined using magnetic resonance imaging/spectroscopy, body composition was analyzed using dual-energy X-ray absorptiometry, and blood parameters were measured by LabCorp. T-tests were used for statistical analysis and considered significant at < 0.05. While there was no significant change in IHL in the LD group, there was a significant 23% reduction in IHL in the HD group ( = 0.02). Fat mass, lean tissue mass, bone mineral content, and blood lipids were not altered. Post-EAAS phosphatidylethanol was elevated and remained unchanged in LD at 407 ± 141 ng/mL and HD at 429 ± 196 ng/mL, indicating chronic and excess alcohol consumption. The HD of the proprietary EAAS formulation consumed BID seemed to lower IHL in individuals with mild to moderate AUD. We suggest that further studies in a larger cohort be conducted to more completely address this important area of investigation.
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http://dx.doi.org/10.3390/nu12010254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019240PMC
January 2020

Essential Amino Acid Supplement Lowers Intrahepatic Lipid despite Excess Alcohol Consumption.

Nutrients 2020 Jan 19;12(1). Epub 2020 Jan 19.

Department of Biology and Wildlife, University of Alaska Fairbanks, Fairbanks, AK 99775, USA.

Excess alcohol consumption is a top risk factor for death and disability. Fatty liver will likely develop and the risk of liver disease increases. We have previously demonstrated that an essential amino acid supplement (EAAS) improved protein synthesis and reduced intrahepatic lipid in the elderly. The purpose of this exploratory pilot study was to initiate the evaluation of EAAS on intrahepatic lipid (IHL), body composition, and blood lipids in individuals with mild to moderate alcohol use disorder (AUD). Following consent, determination of eligibility, and medical screening, 25 participants (18 males at 38 ± 15 years/age and 7 females at 34 ± 18 years/age) were enrolled and randomly assigned to one of two dosages: a low dose (LD: 8 g of EAAS twice/day (BID)) or high dose (HD: 13 g of EAAS BID). Five of the twenty-five enrolled participants dropped out of the intervention. Both groups consumed the supplement BID for 4 weeks. Pre- and post-EAAS administration, IHL was determined using magnetic resonance imaging/spectroscopy, body composition was analyzed using dual-energy X-ray absorptiometry, and blood parameters were measured by LabCorp. T-tests were used for statistical analysis and considered significant at < 0.05. While there was no significant change in IHL in the LD group, there was a significant 23% reduction in IHL in the HD group ( = 0.02). Fat mass, lean tissue mass, bone mineral content, and blood lipids were not altered. Post-EAAS phosphatidylethanol was elevated and remained unchanged in LD at 407 ± 141 ng/mL and HD at 429 ± 196 ng/mL, indicating chronic and excess alcohol consumption. The HD of the proprietary EAAS formulation consumed BID seemed to lower IHL in individuals with mild to moderate AUD. We suggest that further studies in a larger cohort be conducted to more completely address this important area of investigation.
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http://dx.doi.org/10.3390/nu12010254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019240PMC
January 2020

Altered Expression of Adrenomedullin 2 and its Receptor in the Adipose Tissue of Obese Patients.

J Clin Endocrinol Metab 2020 01;105(1)

Department of Surgery, Inha University, College of Medicine, Incheon, 22332, Korea.

Context: Adrenomedullin 2 (AM2) plays protective roles in the renal and cardiovascular systems. Recent studies in experimental animals demonstrated that AM2 is an adipokine with beneficial effects on energy metabolism. However, there is little information regarding AM2 expression in human adipose tissue.

Objective: To investigate the pattern and regulation of the expression of AM2 and its receptor component in human adipose tissue, in the context of obesity and type 2 diabetes.

Methods: We measured metabolic parameters, serum AM2, and expression of ADM2 and its receptor component genes in abdominal subcutaneous and visceral adipose tissue in obese (with or without type 2 diabetes) and normal-weight women. Serum AM2 was assessed before and 6 to 9 months after bariatric surgery. Expression/secretion of AM2 and its receptor were assessed in human adipocytes.

Results: ADM2 mRNA in both fat depots was higher in obese patients, whether diabetic or not. Although serum AM2 was significantly lower in obese patients, it was not changed after bariatric surgery. AM2 and its receptor complex were predominantly expressed by adipocytes, and the expression of CALCRL, encoding a component of the AM2 receptor complex, was lower in both fat depots of obese patients. Incubating adipocytes with substances mimicking the microenvironment of obese adipose tissue increased ADM2 mRNA but reduced both AM2 secretion into culture media and CALCRL mRNA expression.

Conclusions: Our data indicate that AM2 signaling is suppressed in adipose tissue in obesity, involving lower receptor expression and ligand availability, likely contributing to insulin resistance and other aspects of the pathophysiology associated with obesity.
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http://dx.doi.org/10.1210/clinem/dgz066DOI Listing
January 2020

17-Oxo-docosahexaenoic acid induces Nrf2-mediated expression of heme oxygenase-1 in mouse skin in vivo and in cultured murine epidermal cells.

Arch Biochem Biophys 2020 01 17;679:108156. Epub 2019 Oct 17.

Department of Molecular Medicine and Biopharmaceutical Science, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, 08826, South Korea; Tumor Microenvironment Global Core Research Institute, College of Pharmacy, Seoul National University, Seoul, 08826, South Korea; Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, 08826, South Korea; Cancer Research Institute, Seoul National University, Seoul, 03080, South Korea. Electronic address:

Recently, growing attention has been given to new classes of bioactive lipid mediators derived from ω-3 polyunsaturated fatty acids, such as docosahexaenoic acid (DHA), especially in the context of their role as endogenous signal modulators. One such molecule is 17-oxo-DHA, generated from DHA by the action of COX2 and a dehydrogenase. The redox-sensitive transcription factor, Nrf2 plays a key role in cellular stress responses. In the present study, the effects of 17-oxo-DHA on Nrf2-mediated expression of cytoprotective enzymes were examined in mouse skin in vivo and cultured murine epidermal JB6 cells. Topical application of 17-oxo-DHA markedly elevated the nuclear localization of Nrf2 and expression of heme oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase-1 in hairless mouse skin. In contrast to 17-oxo-DHA, the non-electrophilic metabolic precursor 17-hydroxy-DHA was a much weaker inducer of Nrf2 activation and its target protein expression. Likewise, 17-oxo-DHA significantly enhanced nuclear translocation and transcriptional activity of Nrf2 with concomitant upregulation of HO-1 expression in cultured JB6 cells. 17-Oxo-DHA was a much stronger inducer of Nrf2-mediated antioxidant response than its parent molecule, DHA. HO-1 expression was abolished in Nrf2 knockdown JB6 cells or embryo fibroblasts from Nrf2 knock out mice. 17-Oxo-DHA also markedly reduced the level of Keap1 protein by inducing ubiquitination. Mutation of Cys151 and Cys273 in Keap1 abrogated 17-oxo-DHA-induced ubiquitination and proteasome-mediated degradation of Keap1 as well as HO-1 expression, suggesting that these cysteine residues are putative sites for 17-oxo-DHA binding. Further, Keap1 degradation stimulated by 17-oxo-DHA coincided with accumulation of the autophagy substrate, p62/SQSTM1.
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http://dx.doi.org/10.1016/j.abb.2019.108156DOI Listing
January 2020

Kinetic Resolution of Racemic Aldehydes through Asymmetric Allenoate γ-Addition: Synthesis of (+)-Xylogiblactone A.

Org Lett 2019 09 5;21(18):7660-7664. Epub 2019 Sep 5.

Department of Chemistry , Sungkyunkwan University , Suwon 16419 , Republic of Korea.

A synthesis of (+)-xylogiblactone A has been achieved from -butyl 2-methylbuta-2,3-dienoate in a linear three-step sequence. The key elements of the synthesis include a kinetic resolution of racemic 2-silyoxyaldehyde through the allenoate γ-addition to yield the γ-adduct as a single isomer and the subsequent gold catalysis to form the butenolide core. For a general method, the kinetic resolution of several racemic 2-silyloxyaldehydes is also performed to provide products in high levels of stereoselectivity with unusual -Felkin-Anh addition fashion.
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http://dx.doi.org/10.1021/acs.orglett.9b02982DOI Listing
September 2019

1-Palmitoyl-2-Linoleoyl-3-Acetyl--Glycerol Attenuates Streptozotocin-Induced Pancreatic Beta Cell Damage by Promoting Glucose Transporter 2 Endocytosis.

Mol Cell Biol 2019 11 11;39(21). Epub 2019 Oct 11.

Cell Factory Research Center, Division of Systems Biology and Bioengineering, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea

Streptozotocin (STZ) is widely used to induce diabetic rodent models. It is specifically toxic to pancreatic beta cells and causes severe destruction and dysfunction. We investigated the effect of 1-palmitoyl-2-linoleoyl-3-acetyl--glycerol (PLAG) on an STZ-induced diabetic mouse model. PLAG attenuated the glucose increase and maintained serum insulin at levels similar to those seen with control mice. In pancreatic beta cell line INS-1, STZ-induced cell apoptosis and intracellular reactive oxygen species (ROS) generation were significantly reduced to nearly normal levels after PLAG treatment. Glucose transporter 2 (GLUT2) localization analyses and glucose uptake assays showed that PLAG accelerated GLUT2 internalization, which ameliorated excessive entry of glucose, as well as STZ. STZ-induced cytotoxic effects were significantly reduced in PLAG-treated groups. The biological activity of PLAG was further confirmed in GLUT2-silenced cells, and the specificity of PLAG was verified using its derivative 1-palmitoyl-2-linoleoyl-3-hydroxyl--glycerol (PLH). Our results suggest that PLAG may be a useful agent for protecting beta cells in the setting of excessive glucose influx.
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http://dx.doi.org/10.1128/MCB.00157-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6791655PMC
November 2019

Risk for pneumonia requiring hospitalization or emergency room visit according to delivery device for inhaled corticosteroid/long-acting beta-agonist in patients with chronic airway diseases as real-world evidence.

Sci Rep 2019 08 19;9(1):12004. Epub 2019 Aug 19.

Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul Metropolitan Government-Seoul National University Boramae Medical Center, Seoul, Republic of Korea.

A fixed-dose combination of inhaled corticosteroid and long-acting beta agonist (ICS/LABA) may increase the risk of pneumonia in patients with chronic airway diseases including chronic obstructive pulmonary disease and asthma. Although lung deposition of ICS/LABA is dependent on the inhaler device and inhalation technique, there have been few studies comparing the risk for pneumonia according to the type of device used to deliver ICS/LABA in real-world practice. A retrospective cohort study was performed using the National Health Insurance Database of the Korean Health Insurance Review & Assessment Service. New users who began ICS/LABA were selected and followed-up 180 days after ICS/LABA initiation. The risk for pneumonia requiring emergency room (ER) visit or admission was compared according to inhaler device used-pressurized metered-dose inhaler (pMDI) or dry powder inhaler (DPI)-after individual exact matching (1:5). Among the eligible cohort of 245,477 new ICS/LABA users, 7,942 patients who used pMDI only were matched with 39,690 patients who used DPI only. The incidence of pneumonia was higher in the pMDI group (1.6%) than the DPI group (1.1%); the adjusted hazard ratio (HR) for pneumonia was 1.6 (95% CI 1.3-2.0; p < 0.0001). In subgroup analyses, a significantly higher risk for pneumonia was found in the pMDI group compared with the DPI group regardless of the presence of history of pneumonia (HR 1.7 [95% CI 1.2-2.3]; p = 0.002), COPD (HR 1.6 [95% CI 1.2-2.0]; p = 0.0007), or asthma (HR 1.6 [95% CI 1.2-2.2]; p = 0.0008). In analyses of real-world data, pMDI users incurred a higher risk for pneumonia requiring hospitalization or ER visit compared with DPI users.
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http://dx.doi.org/10.1038/s41598-019-48355-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6700062PMC
August 2019

Budget Impact Analysis of the Change in Peritoneal Dialysis Use Rate in Korea.

Perit Dial Int 2019 Nov-Dec;39(6):547-552. Epub 2019 Jul 23.

National Evidence-Based Healthcare Collaborating Agency, Seoul, Korea

While the number of peritoneal dialysis (PD) patients has decreased by 14.4% from 2006 to 2016, the number of hemodialysis (HD) patients has sharply increased, by 237.2%, in the same period, leading to an increase in the total medical cost. We analyzed the effects of the changes in PD use rates for dialysis patients in Korea on the healthcare budget using budget impact analysis (BIA).The analysis modeled the influence of the increase in dialysis for the target population, changes in modality use rate, and/or changes in costs per patient-year on total medical cost for patients on dialysis, using the National Health Insurance Service (NHI) claims data. We developed 8 scenarios according to the changing PD use rate.In scenarios 1 - 4 (increase in PD patients by 6%, 13%, 20%, and 50% of non-diabetic prevalent HD patients under 65), 5-year budget savings ranged from $47 million to $394 million (0.9% - 7.3% of the end-stage renal disease [ESRD] budget). In scenarios 5 - 8 (increase in incident PD patients by 20%, 50%, 70%, and 100% of non-diabetic patients under 65), 5-year savings ranged from $25 million to $74 million (0.5% - 1.4% of the ESRD budget). In all scenarios, budget savings were higher as PD patients increased, showing a gradually growing trend.In all scenarios from the payer's perspective, savings could be achieved through an increase in PD use. Selecting PD for ESRD patients without different expected clinical outcomes between HD and PD would be beneficial to the NHI budget.
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http://dx.doi.org/10.3747/pdi.2018.00037DOI Listing
August 2020

Primary Imaging Test for Suspected Traumatic Thoracolumbar Spine Injury: 2017 Guidelines by the Korean Society of Radiology and National Evidence-Based Healthcare Collaborating Agency.

Korean J Radiol 2019 06;20(6):909-915

Department of Radiology and Research Institute of Radiology, University of Ulsan College of Medicine, Seoul, Korea.

The Korean Society of Radiology and the National Evidence-based Healthcare Collaborating Agency developed a primary imaging test for suspected traumatic thoracolumbar spine injury. This guideline was developed using an adaptation process involving collaboration between the development committee and the working group. The development committee, consisting of research methodology experts, established the overall plan and provided support on research methodology. The working group, composed of radiologists with expertise in musculoskeletal imaging, wrote the recommendation. The guidelines recommend that thoracolumbar spine computed tomography without intravenous contrast enhancement be the first-line imaging modality for diagnosing traumatic thoracolumbar spine injury in adults.
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http://dx.doi.org/10.3348/kjr.2018.0792DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6536791PMC
June 2019

Multi-criteria decision support system of the photovoltaic and solar thermal energy systems using the multi-objective optimization algorithm.

Sci Total Environ 2019 Apr 29;659:1100-1114. Epub 2018 Dec 29.

Department of Architecture and Architectural Engineering, Yonsei University, Seoul 03722, Republic of Korea.

When the photovoltaic (PV) and solar thermal energy (STE) systems, which share the rooftop area, are installed in the same building, a trade-off problem occurs in terms of the energy, economic, and environmental aspects, and thus, steps need to solve this problem. Therefore, this study aimed to develop a multi-criteria decision support system of the PV and STE systems using the multi-objective optimization algorithm. This system was developed in the following six steps: (i) database establishment; (ii) designing the variables of the PV and STE systems; (iii) development of the analysis engine of the PV and STE systems; (iv) environmental and economic assessment from the life cycle perspective; (v) integrated multi-objective optimization (iMOO) with a genetic algorithm; and (vi) establishment of a multi-criteria decision support system. To verify the robustness and reliability of the developed model, an analysis of "D" City Hall and "I" Airport as target facilities was performed. The optimal PV and STE systems that consider the energy, economic, and environmental aspects at the same time were determined with respect to the 1.23 × 10 and 1.05 × 10 installation scenarios, respectively, in terms of effectiveness. The iMOO scores of the existing PV and STE systems installed in "D" City Hall and "I" Airport were 0.358 and 0.346, respectively, whereas those of the optimal solutions were 0.249 and 0.280, showing score improvements. In terms of efficiency, the times required for determining the optimal solutions were 20 and 30 min, respectively. The developed model makes the final decision-maker to find the optimal solution in introducing the PV and STE systems in the early design phase at the same time.
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http://dx.doi.org/10.1016/j.scitotenv.2018.12.387DOI Listing
April 2019

Convergence Despite Divergence: Views of Academic and Community Stakeholders about the Ethics of Community-Engaged Research.

Ethn Dis 2019 18;29(2):309-316. Epub 2019 Apr 18.

UNC Center for Health Equity Research, University of North Carolina School of Medicine.

Purpose: Stakeholder engagement and community-engaged research (CEnR) are recognized as approaches necessary to promote health equity. Few studies have examined variations in stakeholder perspectives on research ethics despite the potential for meaningful differences. Our study examines the association between stakeholders' characteristics and their perception of the importance of 15 stakeholder-developed CEnR ethical statements.

Design: Quantitative analysis of close-ended Delphi survey.

Participants: We recruited a national, non-random, purposive sample of people who were eligible if they endorsed conducting CEnR in public health or biomedical fields. Participants were recruited from publicly available information, professional email distributions, and snowball sampling.

Main Outcome Measures: We designed our close-ended Delphi survey from the results of 15 CEnR ethical statements, which were developed from a consensus development workshop with academic and community stakeholders.

Results: 259 participants completed the Delphi survey. The results demonstrated that stakeholders' characteristics (affiliation, ethnicity, number of CEnR relationships, and duration of CEnR partnerships) were not associated with their perception of the importance of 15 ethical statements.

Conclusions: The strong agreement among stakeholders on these broad, aspirational ethical statements can help guide partnerships toward ethical decisions and actions. Continued research about variability among stakeholders' ethics perspectives is needed to bolster the capacity of CEnR to contribute to health equity.
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http://dx.doi.org/10.18865/ed.29.2.309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6478047PMC
June 2020

Multi-dimensional bioinspired tactics using an engineered mussel protein glue-based nanofiber conduit for accelerated functional nerve regeneration.

Acta Biomater 2019 05 9;90:87-99. Epub 2019 Apr 9.

Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 37673, Republic of Korea. Electronic address:

Limited regenerative capacity of the nervous system makes treating traumatic nerve injuries with conventional polymer-based nerve grafting a challenging task. Consequently, utilizing natural polymers and biomimetic topologies became obvious strategies for nerve conduit designs. As a bioinspired natural polymer from a marine organism, mussel adhesive proteins (MAPs) fused with biofunctional peptides from extracellular matrix (ECM) were engineered for accelerated nerve regeneration by enhancing cell adhesion, proliferation, neural differentiation, and neurite formation. To physically promote contact guidance of neural and Schwann cells and to achieve guided nerve regeneration, MAP was fabricated into an electrospun aligned nanofiber conduit by introducing synthetic polymer poly(lactic-co-glycolic acid) (PLGA) to control solubility and mechanical property. In vitro and in vivo experiments demonstrated that the multi-dimensional tactics of combining adhesiveness from MAP, integrin-mediated interaction from ECM peptides (in particular, IKVAV derived from laminin α1 chain), and contact guidance from aligned nanofibers synergistically accelerated functional nerve regeneration. Thus, MAP-based multi-dimensional approach provides new opportunities for neural regenerative applications including nerve grafting. STATEMENT OF SIGNIFICANCE: Findings in neural regeneration indicate that a bioinspired polymer-based nerve conduit design should harmoniously constitute various factors, such as biocompatibility, neurotrophic molecule, biodegradability, and contact guidance. Here, we engineered three fusion proteins of mussel-derived adhesive protein with ECM-derived biofunctional peptides to simultaneously provide biocompatibility and integrin-based interactions. In addition, a fabrication of robust aligned nanofiber conduits containing the fusion proteins realized suitable biodegradability and contact guidance. Thus, our multi-dimensional strategy on conduit design provided outstanding biocompatibility, biodegradability, integrin-interaction, and contact guidance to achieve an accelerated functional nerve regeneration. We believe that our bioengineered mussel adhesive protein-based multi-dimensional strategy would offer new insights into the design of nerve tissue engineering biomaterials.
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http://dx.doi.org/10.1016/j.actbio.2019.04.018DOI Listing
May 2019

Comparative study of peritoneal dialysis versus hemodialysis on the clinical outcomes in Korea: a population-based approach.

Sci Rep 2019 04 11;9(1):5905. Epub 2019 Apr 11.

Department of Internal Medicine, School of Medicine, Ewha Womans University, Seoul, Korea.

There has been paucity of data regarding the secular trend of adverse outcomes in peritoneal dialysis (PD) as compared with hemodialysis (HD) in Korea. 96,596 patients who started dialysis between 2004-2015 in Korea were identified using the National Health Insurance Service database. The adjusted hazard ratio (HR) (95% confidence interval, CI) of PD over HD for mortality was 1.31 (1.27-1.36; P < 0.001) in the period of 2004-2007 and 1.21 (1.16-1.27; P < 0.001) in the period of 2008-2011. However, the hazard of PD over HD for mortality turned out to be insignificant in the period of 2012-2015. Similar trend was noted for nonfatal cardiovascular events (CVEs). In subgroup analysis, the hazard of PD over HD for mortality was evident, regardless of the status of age, diabetes, and comorbidity burden in 2004-2011. In 2012-2015, however, the hazard of PD over HD for mortality was insignificant when follow up was censored at one year, which became significant when follow up follow up was censored at three or five year. In conclusion, the mortality of PD over HD in Korea has been significantly improved, a finding that was paralleled by the improved nonfatal CVEs.
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http://dx.doi.org/10.1038/s41598-019-42508-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459886PMC
April 2019

Neural Interactome: Interactive Simulation of a Neuronal System.

Front Comput Neurosci 2019 13;13. Epub 2019 Mar 13.

Department of Electrical and Computer Engineering, University of Washington, Seattle, WA, United States.

Connectivity and biophysical processes determine the functionality of neuronal networks. We, therefore, developed a real-time framework, called Neural Interactome, to simultaneously visualize and interact with the structure and dynamics of such networks. Neural Interactome is a cross-platform framework, which combines graph visualization with the simulation of neural dynamics, or experimentally recorded multi neural time series, to allow application of stimuli to neurons to examine network responses. In addition, Neural Interactome supports structural changes, such as disconnection of neurons from the network (ablation feature). Neural dynamics can be explored on a single neuron level (using a zoom feature), back in time (using a review feature), and recorded (using presets feature). The development of the Neural Interactome was guided by generic concepts to be applicable to neuronal networks with different neural connectivity and dynamics. We implement the framework using a model of the nervous system of () nematode, a model organism with resolved connectome and neural dynamics. We show that Neural Interactome assists in studying neural response patterns associated with locomotion and other stimuli. In particular, we demonstrate how stimulation and ablation help in identifying neurons that shape particular dynamics. We examine scenarios that were experimentally studied, such as touch response circuit, and explore new scenarios that did not undergo elaborate experimental studies.
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http://dx.doi.org/10.3389/fncom.2019.00008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425397PMC
March 2019

Negative Energy Balance Does Not Alter Fat-Free Mass During the Yukon Arctic Ultra-The Longest and the Coldest Ultramarathon.

Front Physiol 2018 20;9:1761. Epub 2018 Dec 20.

Charité - Universitätsmedizin Berlin, Institute of Physiology, Center for Space Medicine and Extreme Environments Berlin, Berlin, Germany.

The objective of this study was to determine alterations in caloric balance, body composition, metabolites, and cytokines in athletes participating in the Yukon Arctic Ultra. Ten participants traveling on foot in the 2017 692-km event were recruited for the study. Measurements and samples were obtained at pre-event, 278 km (C1), 384 km (C2), and post-event. Body composition measurements were obtained using bioelectrical impedance analysis. Accelerometer devices were utilized to provide an estimation of caloric expenditure and dietary recalls provided assessments of caloric intake. Blood serum samples were collected, processed, and analyzed using enzyme-linked immunosorbent assays or nuclear magnetic resonance. Results were analyzed using linear mixed model, presented as means ± SD, and considered significant at  < 0.05. Participants (8 males, 2 females; age: 37 ± 10 years; body mass index: 24.4 ± 2.5 kg/m) were recruited. Four males and one female completed the entire event in 260 ± 19 h. Caloric intake/expenditure was 4,126 ± 1,115 kcal/day and 6,387 ± 781 kcal/day, respectively, indicating a caloric deficit of 2,261 ± 1,543 kcal/day. Total mass, body mass index, and fat mass were reduced at each time point of the event. Fat-free mass (FFM) was unchanged throughout the event. Follistatin was increased at C1 (1,715 ± 876 pg/ml) in comparison to baseline. Acetoacetate increased significantly at post-event (6.1 ± 1.5 mg/ml). Despite a pronounced caloric deficit and sustained activity under extreme cold conditions, FFM was preserved with an increase in serum follistatin and acetoacetate. Future studies should be directed at the role of nutrient strategies and/or training methods on the retention of FFM under these conditions.
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http://dx.doi.org/10.3389/fphys.2018.01761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327832PMC
December 2018

The effects of filters for an intelligent air pollutant control system considering natural ventilation and the occupants.

Sci Total Environ 2019 Mar 5;657:410-419. Epub 2018 Dec 5.

Department of Architecture and Architectural Engineering, Yonsei University, Seoul 03722, Republic of Korea.

Experimental analysis was conducted on the indoor air pollutant concentration using natural ventilation and filters. The study targeted two office rooms each of which was occupied by four people, and with the same outdoor environments. A non-woven fabric filter (room A) and an electrostatic filter (room B) were installed on the window frame, and the indoor air pollutant concentration and indoor climate factors were monitored based on the number of occupants and the occupants' activities. The results are as follows: (i) when the number of occupants in each room increased from 0.03-0.06 to 1.53-1.63, room A showed a 60% average PM concentration increase while room B showed an opposite result (10% average PM concentration decrease), meaning the electrostatic filter's lower resistance to flow contributed to better ventilation and also decreased the influence of the occupants on the indoor air pollutant concentration. A low correlation (0.323-0.350) between the CO concentration and the occupants in room B also proved these results; (ii) while the average PM concentration in room A was 9 μg/m higher than that in room B, the average PM concentration in room A was higher by only 0.2 μg/m, which showing that much of the generated or resuspended indoor particulate matter was PM; and (iii) due to the more frequent heat transfer from outdoors to indoors, room B consumed 23% more heating energy. The results of this study are expected to be used as bases for the establishment of an appropriate management strategy that considers the indoor air pollutant concentration caused by the number of occupants and occupants' activities by combining natural ventilation and filters.
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http://dx.doi.org/10.1016/j.scitotenv.2018.12.054DOI Listing
March 2019
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