Publications by authors named "Jiin-Ju Guo"

2 Publications

  • Page 1 of 1

De novo transcriptome analysis of immune response on cobia (Rachycentron canadum) infected with Photobacterium damselae subsp. piscicida revealed inhibition of complement components and involvement of MyD88-independent pathway.

Fish Shellfish Immunol 2018 Jun 23;77:120-130. Epub 2018 Mar 23.

Laboratory of Molecular Fish Immunology and Genetics, Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan; Research Center for Animal Biologics, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan. Electronic address:

Cobia, Rachycentron canadum, one of the most important aquatic species in Taiwan, has suffered heavy losses from Photobacterium damselae subsp. piscicida, which is the causal agent of photobacteriosis. In this study, the transcriptomic profiles of livers and spleens from Pdp-infected and non-infected cobia were obtained for the first time by Illumina-based paired-end sequencing method with a focus on immune-related genes. In total, 164,882 high quality unigenes were obtained in four libraries. Following Pdp infection, 7302 differentially expressed unigenes from liver and 8600 differentially expressed unigenes from spleen were identified. Twenty-seven of the differently expressed genes were further validated by RT-qPCR (average correlation coefficient 0.839, p-value <0.01). Results indicated a negative regulation of complement components and increased expression of genes involved in MyD88-independent pathway. Moreover, a remarkable finding was the increased expression of IL-10, implying an inadequacy of immune responses. This study not only characterized several putative immune pathways, but also provided a better understanding of the molecular responses to photobacteriosis in cobia.
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June 2018

Disposition of 3-(4-cyano-2-oxobutylidene amino)-2-oxazolidone, a cyano-metabolite of furazolidone, in furazolidone-treated grouper.

Food Addit Contam 2003 Mar;20(3):229-36

Institute of Fisheries Science, National Taiwan University, Taipei 106, Taiwan.

The cyano-metabolite of furazolidone (FZ), 3-(4-cyano-2-oxobutylidene amino)-2-oxazolidone, was isolated from the mixture of FZ incubated with the post-9000 g hepatic supernatant of grouper. Its structure was confirmed by mass spectrometric and nuclear magnetic resonance spectroscopic studies. Thereafter, the disposition of the cyano-metabolite in the orange-spotted grouper (Epinephelus coioides) after oral and bath treatment with FZ was investigated. Qualitative and quantitative analyses of cyano-metabolite in the fish were performed by high-performance liquid chromatography. Mean recoveries of the metabolite in serum, muscle, liver and kidney were 99.8 +/- 4.1, 98.6 +/- 3.5, 53.1 +/- 7.4 and 64.0 11.4%, respectively. Cyano-metabolite was mainly distributed in the serum and muscle rather than in the liver and kidney. After oral treatment of FZ, the peak cyano-metabolite concentrations, 167.2 ng x ml(-1) in serum and 283.2 ng x g(-1) in muscle, were reached at 5.1 and 6.7 h, respectively. The elimination half-life of cyano-metabolite was 4 h. During 24-h bath treatment of FZ, the maximum concentrations of cyano-metabolite, 258 ng x ml(-1) in serum and 204 ng x g(-1) in muscle, were found at 0.25 and 6 h, respectively. The half-life of cyano-metabolite was 0.5 h after transferring the fish to fresh seawater.
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March 2003