Publications by authors named "Jihong Shen"

21 Publications

  • Page 1 of 1

Interpreting Depression From Question-wise Long-term Video Recording of SDS Evaluation.

IEEE J Biomed Health Inform 2021 Jun 25;PP. Epub 2021 Jun 25.

Self-Rating Depression Scale (SDS) questionnaire has frequently been used for efficient depression preliminary screening. However, the uncontrollable self-administered measure can be easily affected by insouciantly or deceptively answering, and producing the different results with the clinician-administered Hamilton Depression Rating Scale (HDRS) and the final diagnosis. Clinically, facial expression (FE) and actions play a vital role in clinician-administered evaluation, while FE and action are underexplored for self-administered evaluations. In this work, we collect a novel dataset of 200 subjects to evidence the validity of self-rating questionnaires with their corresponding question-wise video recording. To automatically interpret depression from the SDS evaluation and the paired video, we propose an end-to-end hierarchical framework for the long-term variable-length video, which is also conditioned on the questionnaire results and the answering time. Specifically, we resort to a hierarchical model which utilizes a 3D CNN for local temporal pattern exploration and a redundancy-aware self-attention (RAS) scheme for question-wise global feature aggregation. Targeting for the redundant long-term FE video processing, our RAS is able to effectively exploit the correlations of each video clip within a question set to emphasize the discriminative information and eliminate the redundancy based on feature pair-wise affinity. Then, the question-wise video feature is concatenated with the questionnaire scores for final depression detection. Our thorough evaluations also show the validity of fusing SDS evaluation and its video recording, and the superiority of our framework to the conventional state-of-the-art temporal modeling methods.
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http://dx.doi.org/10.1109/JBHI.2021.3092628DOI Listing
June 2021

Research on Characteristic of Chronic Spontaneous Urticaria Based on Multiscale Entropy.

Comput Math Methods Med 2021 25;2021:6691356. Epub 2021 May 25.

College of Mathematical Sciences, Harbin Engineering University, Harbin 150001, China.

Chronic spontaneous urticaria (CSU) is a common skin disease which symptom is local pruritus and pain. In medicine, researchers take a certain point that the brain is the control center of CSU, but in previous experiments, the researchers found that cerebellum also had a certain effect on CSU. In order to find out the influence of CSU in the brain and cerebellum, we collected the brain resting-state fMRI data from 40 healthy controls and 32 CSU patients and used DPABI to preprocess. We calculated the entropy values of five scales by using multiscale entropy (MSE) and the average entropy values of two groups' BOLD signals; 15 regions with significant differences were found which not only had a more detailed impact in the brain but also had an impact in the cerebellum, such as precentral gyrus, lenticular putamen, and vermis of cerebellum. In addition, we found that compared with the healthy controls, the entropy values of CSU patients showed two trends which need further study. The advantage of our experiment is that the multiscale entropy value is used to get more influence regions of CSU in the brain and cerebellum. The results of this paper may provide some help for the pathological study of CSU.
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http://dx.doi.org/10.1155/2021/6691356DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172304PMC
May 2021

A novel peptide identified from skin secretions of Bombina maxima possesses LPS-neutralizing activity.

Biochem Biophys Res Commun 2021 04 6;550:107-112. Epub 2021 Mar 6.

Department of Urology, The First Affiliated Hospital of Kunming Medical University, Kunming, 650032, Yunnan, China. Electronic address:

Lipopolysaccharide (LPS) is a major pathogenic factor in endotoxin shock or sepsis. Most antibiotics have little clinical anti-endotoxin activity, but some antimicrobial peptides (AMPs) have been shown to be effective in blocking LPS. We identified a novel peptide from the skin secretions of Bombina maxima (B. _maxima) by challenging the skin of frogs with an LPS solution. Peptide 2 has an amino acid sequence of LVGKLLKGAVGDVCGLLPIC. Peptide 2 possesses low hemolytic activity, low cytotoxicity against RAW 264.7 cells, and strong anti-inflammatory activity. Moreover, peptide 2 plays an anti-inflammatory role by inhibiting inflammatory cytokines such as tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6). A biolayer interferometry (BLI) assay indicated that peptide 2 binds to LPS with strong affinity and that this interaction has an affinity constant (K) value of 1.05 × 10 M. A survival study showed that peptide 2 possesses potent LPS-neutralizing activity to protect LPS-treated mice from death. In conclusion, we have identified a potent peptide with LPS neutralizing activity, which lays a foundation for future research and development.
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http://dx.doi.org/10.1016/j.bbrc.2021.02.131DOI Listing
April 2021

Extracellular Matrix-Related Six-lncRNA Signature as a Novel Prognostic Biomarker for Bladder Cancer.

Onco Targets Ther 2020 7;13:12521-12538. Epub 2020 Dec 7.

Department of Urology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, People's Republic of China.

Introduction: Bladder cancer (BC) is the fourth-commones cancer and the sixth-leading cause of cancer-related death among men. However, a lack of reliable biomarkers remains a problem forprognosis and treatment of BC. lncRNAs have been shown to play important roles in various cancers, and have emerged as promising biomarkers for cancer prognosis and treatment.

Methods: In this study, using univariate and multivariate Cox regression analysis, we examined the differential expression profiles of 1,651 lncRNAs in the TCGA BLCA cohort and created a prognostic gene signature composed of six lncRNAs (for , , , , , and ), designed the SMALLL signature.

Results: The SMALLL signature displayed significant prognostic power for overall survival for BC patients in multiple cohorts. Gene Ontology analysis showed that genes coexpressed with the SMALLL signature were associated with the extracellular matrix network, and immune cell-infiltration analysis showed that activated naïve B cells, regulatory T cells, M0 macrophages, eosinophils, resting memory CD4 T cells and resting NK cells were significantly different in high- and low-risk groups. We also confirmed differential expression of the lncRNAs of the SMALLL signature in BC tissue and paracancer normal tissue by qRT-PCR analysis. Cell-invasion and -migration experiments showed that , , , and significantly affected cell invasion and migration.

Conclusion: Our study revealed that the lncRNA signature is an important predictive factor of prognosis and provides a promising biomarker for BC.
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http://dx.doi.org/10.2147/OTT.S284167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733340PMC
December 2020

Diversity of Cultivable Microbes From Soil of the Fildes Peninsula, Antarctica, and Their Potential Application.

Front Microbiol 2020 3;11:570836. Epub 2020 Sep 3.

College of Biological Science and Engineering, Fuzhou University, Fuzhou, China.

To explore the diversity and application potential of Antarctic microorganisms, 1208 strains bacteria and fungi were isolated from 5 samples collected from the Fildes Peninsula during China's 27th and 31st Antarctic expeditions. By using 16S and ITS sequence similarity alignment, 83 strains bacteria belonging to 20 genera and 30 strains fungi belonging to 7 genera were identified. Among them, 1 strains bacteria and 6 strains fungi showed low sequence similarity to the database, suggesting that they might be novel species. Physiological-biochemical characteristics showed that the identified bacteria could utilize many kinds of carbohydrates and that the identified fungi could produce several kinds of extracellular enzymes. The fungal strain MS-19, identified as , possesses the potential to produce antifungal activity agents based on an activity-guided approach. Further isolation yielded four polyketones: versicone A (), versicone B (), 4-methyl-5,6-dihydro-2H-pyran-2-one (), and ()-(+)-sydowic acid (). It should be noted that displayed strong activity against , with an MIC value of 3.91 μg/mL.
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http://dx.doi.org/10.3389/fmicb.2020.570836DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7495136PMC
September 2020

Age-related changes in the association of resting-state fMRI signal variability and global functional connectivity in non-demented healthy people.

Psychiatry Res 2020 09 27;291:113257. Epub 2020 Jun 27.

Division of Interdisciplinary Medicine and Biotechnology, Beth Israel Deaconess Medical Center / Harvard Medical School, Boston, MA, United States; Institute of Brain Sciences, National Yang-Ming University, Taipei, Taiwan; Digital Medicine Center, National Yang-Ming University, Taipei, Taiwan. Electronic address:

Research suggests that the aging relates to variability of resting-state fMRI (rs-fMRI) signal and the functional connectivity. However, the association between the spatial and temporal activity of resting-state fMRI signal was less documented. We recruited 477 healthy Han Chinese participants, who were separated into young, middle and old groups to investigate the relationship between the variability and global functional connectivity (gFC) in different age ranges using standard deviation (SD) of time series and gFC, respectively. Our analysis revealed the changing patterns during healthy aging: 1) 17 brain regions(Olfactory_L, Orbital_L etc.) were identified to have significant association of age with both SD and gFC respectively by linear regression analysis; 2) Two typical associations could be observed between SD and gFC: positive and negative correlations; 3) The variation ratio of SD to gFC was changing with age at the voxel level by using unsupervised clustering method. It is the first time to combine voxel-wise variability and gFC together for the study of age-related changes with rs-fMRI signal. This study may provide a new clue for understanding the synchronization of human brain based on SD and gFC due to the effect of aging.
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http://dx.doi.org/10.1016/j.psychres.2020.113257DOI Listing
September 2020

Antibacterial activity of ruthenium polypyridyl complexes against Staphylococcus aureus and biofilms.

J Biol Inorg Chem 2020 08 20;25(5):747-757. Epub 2020 Jun 20.

School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang, 330013, China.

There is clearly a need for the development of new classes of antimicrobials to fight against multidrug-resistant bacteria. Here, we designed and synthesized of three ruthenium polypyridyl complexes: [Ru(bpy)(BTPIP)](ClO) (Ru(II)-1), [Ru(bpy)(ETPIP)](ClO) (Ru(II)-2) and [Ru(bpy)(CAPIP)](ClO) (Ru(II)-3) (N-N = bpy = 2,2'-bipyridine), their antimicrobial activities against S. aureus were assessed. The lead complexes of this set, Ru(II)-1(MIC = 0.016 mg/mL), was tested against biofilm. We also investigated whether bacteria can easily develop resistance to Ru(II)-1. The result demonstrated that S. aureus could not easily develop resistance to the ruthenium complexes. In addition, aimed to test whether ruthenium complexes treatment could increase the susceptibility of S. aureus to antibiotics, the synergism between Ru(II)-1 and common antibiotics against S. aureus were investigated using the checkerboard method. Interesting, Ru(II)-1 could increased the susceptibility of S. aureus to some aminoglycoside antibiotics(kanamycin and gentamicin). Finally, in vivo bacterial infection treatment studies were also conducted through murine skin infection model. These results confirmed ruthenium complexes have good antimicrobial activity in vitro and in vivo.
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http://dx.doi.org/10.1007/s00775-020-01797-wDOI Listing
August 2020

TBC1D25 Regulates Cardiac Remodeling Through TAK1 Signaling Pathway.

Int J Biol Sci 2020 21;16(8):1335-1348. Epub 2020 Feb 21.

Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Road, Zhengzhou, China.

Cardiac remodeling is a major early event of heart failure, which is regulated by multiple signaling pathways. Here, we demonstrate that TBC1D25 is upregulated during pathological cardiac remodeling. The aim of this study is to determine the role of TBC1D25 in cardiac remodeling and to illustrate the underlying molecular mechanism. Specifically, cardiac remodeling was induced in TBC1D25-KO mice and their wild-type control mice through partial transverse aortic constriction (TAC) of aortic arch. Knockout TBC1D25 exacerbated cardiac hypertrophy, fibrosis and dysfunction. Meanwhile, TBC1D25 overexpression in both H9C2 cells and NRCMs alleviate Angiotensin II-induced cardiomyocyte hypertrophy . Moreover, TBC1D25 deficiency increases the phosphorylation levels of TAK1 and its downstream molecular (JNK and p38), whereas overexpressed TBC1D25 inhibits phosphorylation of TAK1, JNK and p38. And TAK1 is the key molecule in this process. Furthermore, we demonstrated that TBC1D25 could directly interacts with TAK1 by immunoprecipitation assay and GST pull-down assay, and the interaction needs the amino acids from at least 138 to 226 in the C-terminal region of TBC1D25 and from 1 to 300 in the C-terminal region of TAK1. We conclude that TBC1D25 suppresses pathological cardiac remodeling via regulating TAK1-JNK/p38 signaling pathway, which suggests that TBC1D25 will likely become a promising therapeutic target for heart failure.
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http://dx.doi.org/10.7150/ijbs.41130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085222PMC
February 2020

Reduced Glycolysis Contributed to Inhibition of Testis Spermatogenesis in Rats After Chronic Methamphetamine Exposure.

Med Sci Monit 2019 Jul 23;25:5453-5464. Epub 2019 Jul 23.

Department of Urology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China (mainland).

BACKGROUND Previous reports suggested that methamphetamine (METH) exposure could lead to inhibition of rat testis spermatogenesis. Glycolysis and glucose metabolism as well as oxidative stress have been implicated in testis spermatogenesis. Here we explored the underlying mechanism of local metabolism and glycolysis of testis after METH exposure. MATERIAL AND METHODS METH was intraperitoneally injected into rats with different doses and duration of METH exposure to establish short-term and chronic exposure models. The serum 8-hydroxy-2 deoxyguanosine (8-OHdG) level of rats was detected by enzyme-linked immunosorbent assay. Untargeted gas chromatography-mass spectrometry analysis was applied to identify differential metabolites and metabolic signature. The mRNA expression of hypoxia inducible factor 1alpha (HIF1alpha), glucose transporter 1 (GLUT1), hexokinase 1 (HK1) and lactate dehydrogenase C (LDHC) in rat testes were detected by polymerase chain reaction. Further, we determined the 4 proteins with western blotting and immunohistochemistry. RESULTS Decreased testes index and sperm counts were showed in the chronic METH group. The metabolome revealed that the main differential metabolites impacted were associated with glycolysis and glucose metabolism. The mRNA and protein expression of GLUT1, HK1, and LDHC were reduced in the chronic METH group but elevated in the short-term METH group, whereas HIF1alpha was upregulated in the short-term METH group but remained at baseline in the chronic METH group. CONCLUSIONS Overall, glucose metabolism was regulated by HIF1alpha after short-term METH exposure. Reduced glycolysis in the testis led to impaired spermatogenesis after chronic METH exposure.
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http://dx.doi.org/10.12659/MSM.917491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6668493PMC
July 2019

A novel AR translational regulator lncRNA LBCS inhibits castration resistance of prostate cancer.

Mol Cancer 2019 06 20;18(1):109. Epub 2019 Jun 20.

Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107. W. Yanjiang Road, Guangzhou, 510120, China.

Background: Progression to a castration resistance state is the main cause of deaths in prostate cancer (PCa) patients. Androgen Receptor (AR) signaling plays the central role in progression of Castration Resistant Prostate Cancer (CRPC), therefore understanding the mechanisms of AR activation in the milieu of low androgen is critical to discover novel approach to treat CRPC.

Methods: Firstly, we explore the CRPC associated lncRNAs by transcriptome microarray. The expression and clinical features of lnc-LBCS are analyzed in three independent large-scale cohorts. The functional role and mechanism of lnc-LBCS are further investigated by gain and loss of function assays in vitro.

Results: The expression of Lnc-LBCS was lower in CRPC cells lines and tissues. LBCS downregulation was correlated with higher Gleason Score, T stage and poor prognosis of PCa patients. LBCS overexpression decreases, whereas LBCS knockdown increases, the traits of castration resistance in prostate cancer cells under androgen ablated or AR blocked condition. Moreover, knockdown of LBCS was sufficient to activate AR signaling in the absence of androgen by elevating the translation of AR protein. Mechanistically, LBCS interacted directly with hnRNPK to suppress AR translation efficiency by forming complex with hnRNPK and AR mRNA.

Conclusions: Lnc-LBCS functions as a novel AR translational regulator that suppresses castration resistance of prostate cancer by interacting with hnRNPK. This sheds a new insight into the regulation of CRPC by lncRNA mediated AR activation and LBCS-hnRNPK-AR axis provides a promising approach to the treatment of CRPC.
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http://dx.doi.org/10.1186/s12943-019-1037-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6585145PMC
June 2019

Association between vitamin D receptor gene polymorphisms and idiopathic hypocitraturia in a Chinese Bai population.

Urolithiasis 2019 Jun 20;47(3):235-242. Epub 2018 Jun 20.

Department of Urology, The First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, Kunming, 650032, China.

Idiopathic hypocitraturia (IH) is a risk factor for urolithiasis. IH is associated with vitamin D receptor (VDR) gene single nucleotide polymorphisms (SNPs) in a Chinese Han population. However, this association between VDR SNPs and IH has not been recapitulated in a Chinese Bai population. The aim of this study is to investigate the association between VDR SNPs and IH in a Chinese Bai population. A total of 320 participants comprising of 200 Chinese Bai patients with IH and 120 Chinese Bai control participants with normal urinary citrate level were enrolled for this study. The VDR SNPs rs7975232, rs2228570, rs731236 and rs1544410 were detected by Sanger sequencing, and the association between these SNPs and the presence of IH in the Chinese Bai population was analyzed. The prevalence of VDR SNPs rs7975232 allele A and rs2228570 genotype TT was significantly higher in patients than in controls (p < 0.0125, after Bonferroni correction). The haplotype TCGC was a protective factor in the Chinese Bai population who otherwise might suffer from IH, while the haplotype TTGA was a risk factor. VDR SNPs rs731236 and rs1544410 have a linkage disequilibrium value of 0.811. VDR SNPs rs7975232, rs2228570, and haplotypes TCGC, TTGA are associated with IH in a Chinese Bai population.
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http://dx.doi.org/10.1007/s00240-018-1069-3DOI Listing
June 2019

Isolation, characterization and transcriptome analysis of a novel Antarctic Aspergillus sydowii strain MS-19 as a potential lignocellulosic enzyme source.

BMC Microbiol 2017 05 30;17(1):129. Epub 2017 May 30.

The First Institute of Oceanography, State Oceanic Administration, Qingdao, 266061, People's Republic of China.

Background: With the growing demand for fossil fuels and the severe energy crisis, lignocellulose is widely regarded as a promising cost-effective renewable resource for ethanol production, and the use of lignocellulose residues as raw material is remarkable. Polar organisms have important value in scientific research and development for their novelty, uniqueness and diversity.

Results: In this study, a fungus Aspergillus sydowii MS-19, with the potential for lignocellulose degradation was screened out and isolated from an Antarctic region. The growth profile of Aspergillus sydowii MS-19 was measured, revealing that Aspergillus sydowii MS-19 could utilize lignin as a sole carbon source. Its ability to synthesize low-temperature lignin peroxidase (Lip) and manganese peroxidase (Mnp) enzymes was verified, and the properties of these enzymes were also investigated. High-throughput sequencing was employed to identify and characterize the transcriptome of Aspergillus sydowii MS-19. Carbohydrate-Active Enzymes (CAZyme)-annotated genes in Aspergillus sydowii MS-19 were compared with those in the brown-rot fungus representative species, Postia placenta and Penicillium decumbens. There were 701CAZymes annotated in Aspergillus sydowii MS-19, including 17 cellulases and 19 feruloyl esterases related to lignocellulose-degradation. Remarkably, one sequence annotated as laccase was obtained, which can degrade lignin. Three peroxidase sequences sharing a similar structure with typical lignin peroxidase and manganese peroxidase were also found and annotated as haem-binding peroxidase, glutathione peroxidase and catalase-peroxidase.

Conclusions: In this study, the fungus Aspergillus sydowii MS-19 was isolated and shown to synthesize low-temperature lignin-degrading enzymes: lignin peroxidase (Lip) and manganese peroxidase (Mnp). These findings provide useful information to improve our understanding of low-temperature lignocellulosic enzyme production by polar microorganisms and to facilitate research and applications of the novel Antarctic Aspergillus sydowii strain MS-19 as a potential lignocellulosic enzyme source.
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http://dx.doi.org/10.1186/s12866-017-1028-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450402PMC
May 2017

Molecular characterization and functional analysis of four teleostean K channels in macrophages of sea perch (Lateolabrax japonicas).

Fish Shellfish Immunol 2017 Jan 12;60:426-435. Epub 2016 Oct 12.

The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, P.R. China.

Potassium ion channels are one of the most diversely and widely distributed channels, which are involved in all kinds of physiological functions in both excitable and non-excitable cells. The expression of voltage-gated potassium ion (Kv) channels is highly variable according to the state of macrophages activation. Macrophages have an important function in innate immunity against intruding pathogens. They produce a variety of inflammatory and immunoactive molecules that modulate imflammatory responses. Here we show that blockade of K channels by non-selective Kv channel inhibitor tetraethylammonium chloride (TEA), and 4-aminopyridine (4-AP) inhibited proinflammatory cytokines expression, cell proliferation, and reactive oxygen species (ROS) production in LPS-stimulated macrophages of Sea perch (Lateolabrax japonicas). Then we isolated four Kv channels genes (spKv1.1, spKv1.2, spKv1.5 and spKv3.1) in LPS-activated fish macrophages. These channels genes were up-regulated after LPS stimulation except spKv3.1, which remained unchanged during the test. The results of this study indicate that Kv channels could be required for modulating the immune function of fish macrophages.
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http://dx.doi.org/10.1016/j.fsi.2016.09.040DOI Listing
January 2017

MicroRNA-200 Family Profile: A Promising Ancillary Tool for Accurate Cancer Diagnosis.

Am J Ther 2016 Mar-Apr;23(2):e388-97

1Department of Urology, the First Affiliated Hospital of Kunming Medical University, Kunming, China; and 2Department of Urology, Qujing Second People's of Yunnan Province, Qujing, China.

Cancer is one of the most threatening diseases in the world and great interests have been paid to discover accurate and noninvasive methods for cancer diagnosis. The value of microRNA-200 (miRNA-200, miR-200) family has been revealed in many studies. However, the results from various studies were inconsistent, and thus a meta-analysis was designed and performed to assess the overall value of miRNA200 in cancer diagnosis. Relevant studies were searched electronically from the following databases: PubMed, Embase, Web of Science, the Cochrane Library, and Chinese National Knowledge Infrastructure. Keyword combined with "miR-200," "cancer," and "diagnosis" in any fields was used for searching relevant studies. Then, the pooled sensitivity, specificity, area under the curve (AUC), and partial AUC were calculated using the random-effects model. Heterogeneity among individual studies was also explored by subgroup analyses. A total of 28 studies from 18 articles with an overall sample size of 3676 subjects (2097 patients and 1579 controls) were included in this meta-analysis. The overall sensitivity and specificity with 95% confidence intervals (95% CIs) are 0.709 (95% CI: 0.657-0.755) and 0.667 (95% CI: 0.617-0.713), respectively. Additionally, AUC and partial AUC for the pooled data is 0.735 and 0.627, respectively. Subgroup analyses revealed that using miRNA-200 family for cancer diagnosis is more effective in white than in Asian ethnic groups. In addition, cancer diagnosis by miRNA using circulating specimen is more effective than that using noncirculating specimen. Finally, miRNA is more accurate in diagnosing endometrial cancer than other types of cancer, and some miRNA family members (miR-200b and miR-429) have superior diagnostic accuracy than other miR-200 family members. In conclusion, the profiling of miRNA-200 family is likely to be a valuable tool in cancer detection and diagnosis.
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http://dx.doi.org/10.1097/MJT.0000000000000361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885590PMC
December 2016

[The characteristics of RR-Lorenz plot in persistent atrial fibrillation patients complicating with escape beats and rhythm].

Zhonghua Xin Xue Guan Bing Za Zhi 2014 Jun;42(6):481-3

Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014, China.

Objective: To explore the characteristics of RR-Lorenz plot in persistent atrial fibrillation (AF) patients complicating with escape beats and rhythm though ambulatory electrocardiogram.

Methods: The 24-hour ambulatory electrocardiogram of 291 persistent AF patients in second affiliated hospital of Zhengzhou university from July 2005 to April 2013 were retrospectively analyzed and the RR interval and the QRS wave were measured. Patients were divided into two groups according to the distribution of the RR-Lorenz point [AF without escape beats and rhythm group (Group A, n = 259) and AF with escape beats and rhythm group (Group B, n = 32)]. The characteristics of RR-Lorenz plot between the two groups were compared.

Results: (1) Fan-shaped RR-Lorenz plots were evidenced in Group A. (2)In Group B, 30 cases showed fan-shaped with L-shaped and a short dense rods along 45° line. The proportion of escape beats and rhythm was 0.28% (275/98 369) -14.06% (11 263/80 112) . The other 2 cases in group B showed no typical RR-Lorenz plots features.

Conclusion: RR-Lorenz plot could help to quickly diagnose persistent AF complicating with escape beats and rhythm according to the typical RR-Lorenz plot characteristics in 24-hour ambulatory electrocardiogram.
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June 2014

Optimization of cold-active lipase production from psychrophilic bacterium Moritella sp. 2-5-10-1 by statistical experimental methods.

Biosci Biotechnol Biochem 2013 7;77(1):17-21. Epub 2013 Jan 7.

School of Marine and Technology, Harbin Institute of Technology, Weihai, PR China.

Statistical experimental designs were applied to optimize cold-active lipase production by the psychrophilic bacterium Moritella sp. 2-5-10-1. First, a Plackett-Burmen design (PBD) was used to evaluate the significant effects of various fermentation parameters. The results indicated that soybean meal, temperature, and Tween-80 had significant influences on lipase production. The levels of these variables were optimized subsequently using central composite design (CCD). A quadratic regression model of cold-active lipase production was built, and verification experiments confirmed its validity. On subsequent scale-up in a 10-L bioreactor using optimized conditions, cold-active lipase production (30.56 U/mL) was obtained. The results clearly indicated that the model was adequate even on a large scale. To our knowledge, this is the first report of statistical optimization of cold-active lipase production by a psychrophilic bacterium.
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http://dx.doi.org/10.1271/bbb.120104DOI Listing
July 2013

Molecular cloning and functional analysis of a voltage-gated potassium channel in lymphocytes from sea perch, Lateolabrax japonicus.

Fish Shellfish Immunol 2012 Sep 29;33(3):605-13. Epub 2012 May 29.

Key Laboratory of Marine Bioactive Substance, State Oceanic Administration, Qingdao 266061, PR China.

Voltage-gated potassium (Kv) channels on cell plasma membrane play an important role in both excitable cells and non-excitable cells and Kv1 subfamily is most extensively studied channel in mammalian cells. Recently, this potassium channel was reported to control processes inside mammalian T lymphocytes such as cell proliferation and volume regulation. Little is known about Kv1 channels in fish. We have postulated the presence of such a channel in lymphocytes and speculated its potential role in immunoregulation in fish. Employing specific primers and RNA template, we cloned a segment of a novel gene from sea perch blood sample and subsequently obtained a full cDNA sequence using RACE approach. Bioinformatic analysis revealed structural and phylogenetic characteristics of a novel Kv channel gene, designated as spKv1.3, which exhibits homologous domains to the members of Kv1.3 family, but it differs notably from some other members of that family at the carboxyl terminus. Full-length of spKv1.3 cDNA is 2152 bp with a 1440 bp open reading frame encoding a protein of 480 amino acids. SpKv1.3 gene is expressed in all of the tested organs and tissues of sea perch. To assess the postulated immune function of spKv1.3, we stimulated lymphocytes with LPS and/or channel blocker 4-AP. Expression levels of messenger RNA (mRNA) of spKv1.3 under stimulation conditions were measured by quantitative RT-PCR. The results showed that LPS can motivate the up-regulation of spKv1.3 expression significantly. Interestingly, we found for the first time that 4-AP with LPS can also increase the spKv1.3 mRNA expression levels in time course. Although 4-AP could block potassium channels physically, we speculated that its effect on blockage of potassium channel may start up an alternative mechanism which feed back and evoke the spKv1.3 mRNA expression.
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http://dx.doi.org/10.1016/j.fsi.2012.05.024DOI Listing
September 2012

A novel omega-3 fatty acid desaturase involved in acclimation processes of polar condition from Antarctic ice algae Chlamydomonas sp. ICE-L.

Mar Biotechnol (NY) 2011 Jun 29;13(3):393-401. Epub 2010 Jul 29.

National Glycoengineering Research Center and College of Life Science, Shandong University, Jinan, 250100, People's Republic of China.

The ability of Antarctic ice algae, Chlamydomonas sp. ICE-L, to survive and proliferate at low temperature and high salinity implies that they have overcome key barriers inherent in Antarctic environments. A full-length complementary DNA (cDNA) sequence of omega-3 fatty acid desaturase, designated CiFAD3, was isolated via reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The full-length of CiFAD3 cDNA contained an open reading frame of 1,302 bp with 5'-terminal untranslated region (UTR) of 36 bp and 3'-terminal UTR of 507 bp encoding a fatty acid desaturase protein of 434 amino acids. Sequence alignment and phylogenetic analysis showed that the gene was homologous to known chloroplastic omega-3 fatty acid desaturase. Meanwhile, CiFAD3 sequence showed typical features of membrane-bound desaturase such as three conserved histidine boxes along with four membrane spanning regions that were universally present among plant desaturases. Under different stress conditions, messenger RNA (mRNA) expression levels of CiFAD3 were measured by quantitative RT-PCR. The results showed that both temperature and salinity could motivate the upregulation of CiFAD3 expression. The mRNA accumulation of CiFAD3 increased 2.6-fold at 0°C and 1.8-fold at 12°C compared to the algae at 6°C. Similarly, mRNA expression levels of CiFAD3 increased 3.8-fold after 62‰ NaCl treatment for 2 h. However, CiFAD3 mRNA expression levels were partially decreased after UV radiation. These data suggest that CiFAD3 is the enzyme responsible for the omega-3 fatty acid desaturation involved in ice algae Chlamydomonas sp. ICE-L acclimatizing to cold temperature and high salinity in Antarctic environment.
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http://dx.doi.org/10.1007/s10126-010-9309-8DOI Listing
June 2011

Molecular cloning and expression analysis of a cytosolic Hsp70 gene from Antarctic ice algae Chlamydomonas sp. ICE-L.

Extremophiles 2010 May 7;14(3):329-37. Epub 2010 Apr 7.

Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, 6 Xianxialing Road, Hightech Park, Qingdao, Shandong, 266061, People's Republic of China.

A cDNA encoding heat shock protein 70 of Antarctic ice algae Chlamydomonas sp. ICE-L (designated as CiHsp70) was identified by RT-PCR and rapid amplification of cDNA ends approaches. The full-length cDNA of CiHsp70 was 2,232 bp, consisting of a 5'-terminal untranslated region (UTR) of 76 bp, a 3'-terminal UTR of 203 bp with a poly (A) tail, and an open reading frame of 1,953 bp. The CiHsp70 cDNA encoded a polypeptide of 651 amino acids with an ATPase domain of 388 amino acids, the substrate peptide binding domain of 246 amino acids and a C-terminus domain of 17 amino acids. The inducible CiHsp70 cDNA was highly homologous to other plant cytosolic Hsp70 genes and clustered together with green algae and higher plant rather than brown algae, diatom and Cryptophyta. Antarctic ice algae were treated with different stress conditions and messenger RNA (mRNA) expression levels of CiHsp70 were quantified by quantitative RT-PCR. The results showed that both cold and heat shock treatments could stimulate CiHsp70 mRNA expression. Meanwhile, CiHsp70 mRNA expression level increased 2.9-fold in response to UV-B radiation for 6 h, while the expression levels of CiHsp70 were remarkably increased after removing the UV-B radiation and immediately providing additional 6 h visible light. Furthermore, treating with 62 or 93 per thousand NaCl for 2 h, CiHsp70 mRNA expression level increased 3.0- and 2.1-fold, respectively. Together, our observations revealed that CiHsp70 as a molecular chaperone might play an important role in Antarctic ice algae Chlamydomonas sp. ICE-L acclimatizing to polar environment.
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http://dx.doi.org/10.1007/s00792-010-0313-8DOI Listing
May 2010

Biodegradation of crude oil by an Arctic psychrotrophic bacterium Pseudoalteromomas sp. P29.

Curr Microbiol 2009 Sep 20;59(3):341-5. Epub 2009 Jun 20.

First Institute of Oceanography, SOA, Key Lab of Marine Bioactive Substances, Qingdao, China.

A psychrotrophic petroleum-degrading bacterium Pseudoalteromonas sp. P29 was isolated from marine sediment, which was collected during 2nd Chinese Arctic Scientific Expedition. The phenotypic character and biodegradation efficiency on mixed oil or vacuum oil were tested at low temperature. The strain Pseudoalteromonas sp. P29 grew in a range of temperature from 5 to 35 degrees C and the optimum temperature was 25 degrees C. Gas chromatography analysis indicated that the strain might preferentially metabolize shorter-chain alkanes. The biodegradation efficiency were nearly 90 and 80%, respectively, after incubation at 5 degrees C for 28 days in the mineral medium supplement with mixed oil or vacuum oil as the sole carbon and energy source. The results showed a possible exploitation of the strain in future biotechnological processes especially in cold contaminated environments.
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http://dx.doi.org/10.1007/s00284-009-9440-9DOI Listing
September 2009

[Cultivation of fibroblasts derived from urethral scar and the effects of salviae miltiorrhizae and ligustrazine on its growth].

Hua Xi Yi Ke Da Xue Xue Bao 2002 Apr;33(2):220-2

Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.

Objective: To explore the possible effect and related mechanism of salviae miltiorrhizae (SM) and ligustrazine in the treatment of urethral scar.

Methods: In vitro cultivation of fibroblasts derived from urethral scar has been developed. The effects of these two medicines on its morphology and proliferation have been detected based on the in vitro culture system.

Results: The spindle cells were found to become elliptical in shape. The two medicines inhibited the proliferation of fibroblasts derived from urethral scar, and this may be resulted from their inhibitive effects on cell division rather than cell necrosis or degradation.

Conclusion: Salviae multiorrhizae and ligustrazine as effective scar inhibitors may have application prospects in the treatment of urethral stricture.
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April 2002
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