Publications by authors named "Jihong Shao"

7 Publications

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Comparative analysis of the main outer membrane proteins of Brucella in the diagnosis of brucellosis.

Biochem Biophys Res Commun 2021 Jun 11;560:126-131. Epub 2021 May 11.

Key Lab of Environment and Health, School of Public Health, Xuzhou Medical University, Xuzhou, 221004, China. Electronic address:

Brucellosis has placed a heavy economic burden on numerous countries and has consumed considerable medical resources worldwide. To improve the specificity and sensitivity of serological methods for diagnosing brucellosis, it is important to develop new diagnostic antigens. Brucella outer membrane proteins(omps) possess good immunogenicity, but there is a scarcity of comparative studies of these proteins in the clinical diagnosis of brucellosis. In this study, six recombinant Brucella outer membrane proteins, omp10, omp16, omp19, omp25, omp31 and BP26, were expressed in prokaryotic cells and utilized as diagnostic antigens. The clinical sera of humans, bovines and goats with brucellosis were analyzed by indirect ELISA using these proteins, lipopolysaccharide(LPS) and Rose Bengale Ag, served as positive-control antigens. In diagnosing human and goat serum, BP26 exhibited the highest diagnostic accuracy of 96.45% and 95.00%, respectively, while omp31 exhibited the strongest ability to detect Brucella in bovine serum with an accuracy of 84.03%. Cross-reaction experiments also confirmed that the diagnostic specificities of omp31 and BP26 were higher than those of the LPS and Rose Bengale Ag antigens. The results of this study indicate that omp31 and BP26 are candidate antigens with high potential application value in the clinical diagnosis of brucellosis.
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http://dx.doi.org/10.1016/j.bbrc.2021.04.127DOI Listing
June 2021

Dietary effects on breast cancer molecular subtypes, a 1:2 paired case-control study.

Food Sci Nutr 2020 Oct 31;8(10):5545-5549. Epub 2020 Aug 31.

Department of Nutrition School of Public Health Xuzhou Medical University Xuzhou China.

To explore the associations between dietary factors and breast cancer (BC) molecular subtypes. The retrospective cases were confirmed by pathological diagnosis with breast cancer were gathered in two major hospitals in Xuzhou city, China, from 2015 to 2016. These cases were classified by the meeting standard of 13th St Gallen: luminal A, luminal B, Her-2 overexpression, and triple-negative breast cancer (TNBC) subtypes. A 1:2 paired retrospective case-control study with 210 cases and 420 controls was conducted to evaluate individual dietary intake, by food frequency questionnaire (FFQ) and estimate odds ratios (s), by the Cox regression model. For overall breast cancer patients, the more frequency of red meat ( = 1.002,  = 1.001-1.004) and salted food ( = 1.003,  = 1.001-1.005) were statistically significantly associated with a greater risk of breast cancer. Beans ( = 0.997,  = 0.995-0.999), white meat ( = 0.993,  = 0.989-0.997), aquatic products ( = 0.990,  = 0.984-0.996), vegetables ( = 0.999,  = 0.999-0.999), fruit ( = 0.998,  = 0.997-0.999), and green tea ( = 0.997,  = 0.994-0.999) were significantly associated with a lower risk of breast cancer. For luminal breast cancer patients, beans ( = 0.997,  = 0.994-0.999), white meat ( = 0.992,  = 0.987-0.997), green tea ( = 0.995,  = 0.991-0.999), and milk ( = 0.998,  = 0.996-0.999) were protective factors. While for nonluminal breast cancer, red meat was not included in the equation, and beans ( = 0.989,  = 0.981-0.997), white meat ( = 0.989,  = 0.981-0.998), vegetables ( = 0.998,  = 0.997-0.999), and milk ( = 0.994,  = 0.989-0.999) still showed a significantly reduced risk of nonluminal breast cancer. Different dietary factors revealed different effects on the etiology of breast cancer. Red meat may be a specific risk factor for luminal-type breast cancer.
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http://dx.doi.org/10.1002/fsn3.1866DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590303PMC
October 2020

Vegetable-Fruit-Soybean Dietary Pattern and Breast Cancer: A Meta-Analysis of Observational Studies.

J Nutr Sci Vitaminol (Tokyo) 2019 ;65(5):375-382

Xuzhou Medical University.

Breast cancer is one of the most common cancers among women worldwide, and several studies have investigated the association of dietary patterns and breast cancer. However, findings of studies are inconclusive. Therefore, we aimed to conduct a meta-analysis to summarize the available data regarding the association of vegetable-fruit-soybean dietary pattern and breast cancer. A systematic literature search was conducted via PubMed, Web of Science and EMBASE to identify eligible cohort studies before February 2019. A total of 12 cohort studies were included in the meta-analysis. The summary relative risks (RR) with 95% CI were calculated with a fixed-effects model. The overall RR of breast cancer for the highest versus lowest intake of vegetable-fruit-soybean dietary pattern was 0.87 (95% CI, 0.82-0.91), with little heterogeneity (p=0.73, I=0%). There was no obvious publication bias according to funnel plot and Begg's and Egger's test. In summary, the evidence from this meta-analysis indicates that vegetable-fruit-soybean dietary pattern was inversely associated with breast cancer. However, well-designed randomized controlled trials are needed to elicit the clear effect of vegetable-fruit-soybean dietary pattern and breast cancer. Women can reduce the risks of breast cancer by eating more fruits and vegetables and soybeans, which is a constructive suggestion.
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http://dx.doi.org/10.3177/jnsv.65.375DOI Listing
March 2020

Lead acetate induces apoptosis in Leydig cells by activating PPARγ/caspase-3/PARP pathway.

Int J Environ Health Res 2021 Jan 30;31(1):34-44. Epub 2019 May 30.

Department of Hygiene,School of Public Health, Xuzhou Medical University , China.

This study was designed to investigate the cytotoxicity of lead acetate (Pb(AC), a representative air pollutant) by focusing on PPARγ/caspase-3/PARP apoptotic signaling pathway and to explore the inhibitory effect of PPARγ antagonist on apoptosis of TM3 Leydig cells. MTT assay was utilized to examine cell viability. Cell apoptosis was analyzed using a flow cytometry by staining with Annexin V-PE/7AAD staining and a fluorescence microscope by staining with Hoechst 33,258. The levels of apoptosis-related proteins were examined using western blot. From the results, Pb reduced significantly TM3 cell proliferation in concentration- and time-dependent manner. It increased significantly apoptosis; increased the PPARγ, Bax, procaspase-3, cleaved caspase-3, proPARP, cleaved PARP levels; and decreased Bcl-2 level in Pb-treated TM3 cells as compared to control cells. Furthermore, pretreatment with PPARγ antagonist significantly attenuated the apoptosis and cleavage of caspase-3 and PARP induced by Pb. Our results suggested that Pb induced cytotoxicity on TM3 Leydig cells, at least in part, by increasing PPARγ expression, stimulating cleavage of caspase-3 and PARP, and then induced cell apoptosis.
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http://dx.doi.org/10.1080/09603123.2019.1625034DOI Listing
January 2021

Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway.

Int J Food Sci Nutr 2019 Aug 9;70(5):570-578. Epub 2019 Jan 9.

b Department of Nutrition and Food Safety School of Public Health , Nanjing Medical University , Nanjing , China.

Sulforaphane (SFN) is the major component extracted from broccoli/broccoli sprouts. It has been shown to possess anti-cancer activity. Gastric cancer is common cancer worldwide. The objective of this work was to evaluate the inhibitory effect of SFN on gastric cancer by Sonic hedgehog (Hh) Pathway. The results found that tumorsphere formation and the expression levels of gastric cancer stem cells (CSCs) markers were significantly decreased after SFN treatment. SFN also exerted inhibitory effects by suppressing proliferation and inducing apoptosis in gastric CSCs. Intriguingly, SFN inhibited the activation of Sonic Hh, a key pathway in maintaining the stemness of gastric CSCs. Upregulation of Sonic Hh pathway diminished the inhibitory effects of SFN on gastric CSCs. Collectively, these data revealed that SFN could be a potent natural compound targeting gastric CSCs via suppression of Sonic Hh pathway, which might be an promising agent for gastric cancer intervention.
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http://dx.doi.org/10.1080/09637486.2018.1545012DOI Listing
August 2019

Genetic Diversity, Antimicrobial Susceptibility, and Biofilm Formation of spp. Recovered from Spices and Cereals.

Front Microbiol 2017 19;8:2567. Epub 2017 Dec 19.

School of Public Health, Xuzhou Medical University, Xuzhou, China.

species are important food-borne opportunistic pathogens which have been implicated in the cause of necrotizing enterocolitis, sepsis, and meningitis in neonates and infants. However, these bacteria are routinely found in foodstuffs, clinical specimens, and environmental samples. This study investigated the genetic diversity, antimicrobial susceptibility, and biofilm formation of isolates ( = 40) recovered from spices and cereals in China during 2014-2015. Based on the A sequencing analysis, we found that the majority (23/40, 57.5%) of isolates in spices and cereals were , while the remaining strains were (6/40, 15.0%), (5/40, 12.5%), (4/40, 10.0%), and (2/40, 5.0%). Multilocus sequence typing (MLST) analysis produced 30 sequence types (STs) among the 40 isolates, with 5 STs (ST4, ST13, ST50, ST129, and ST158) related to neonatal meningitis. The pattern of the overall ST distribution was diverse; in particular, it was revealed that ST148 was the predominant ST, presenting 12.5% within the whole population. MLST assigned 12 isolates to 7 different clonal complexes (CCs), 4, 13, 16, 17, 72, 129, and 143, respectively. The results of O-antigen serotyping indicated that serotype O1 and O2 were the most two prevalent serotypes. The antimicrobial susceptibility testing showed that the 40 isolates were susceptible to most of the antibiotics tested except for ceftriaxone, meropenem, and aztreona. Of the 40 strains tested, 13 (32.5%) were assessed as weak bioflim producers, one (2.5%) was a moderate biofilm producer, one (2.5%) was strong biofilm producer, and the others (62.5%) were non-biofilm producers. MLST and O-antigen serotyping have indicated that strains recovered from spices and cereals were genetically diverse. Isolates of clinical origin, particularly the ST4 neonatal meningitic pathovar, have been identified from spices and cereals. Moreover, antimicrobial resistance of strains was observed, which may imply a potential public health risk. Therefore, the surveillance of spp. in spices and cereals should be strengthened to improve epidemiological understandings of infections.
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http://dx.doi.org/10.3389/fmicb.2017.02567DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5742210PMC
December 2017

[Principle of "multiple ecological niche of one-species (variety)" and its application in high yield cotton cultivation].

Ying Yong Sheng Tai Xue Bao 2004 Jul;15(7):1285-8

Key Laboratory of Oasis Ecology Agriculture of Xinjiang Bingtuan, Agricultural College, Shihezi University, Shihezi 832003, China.

The multiple ecological niche of one-species (variety) (MENOS) population is formed by one species (variety), but the ecological units have different temporal, spatial, nutritional, temperature, or water ecological niche. The formation of MENOS population is characterized by its specific object, one species (variety), multiple layer canopy, human being's active adjustment, specific niche breadth, and overlap and separation of ecological units. This paper analyzed the characteristics of the super high yield cotton population with dual ecological niche of one-species (variety) (DENOS). As for the yield components, MENOS has the characteristics of high yield canopy and different yield plants.
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July 2004