Publications by authors named "Jie Zhou"

2,270 Publications

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Crab bioturbation alters nitrogen cycling and promotes nitrous oxide emission in intertidal wetlands: Influence and microbial mechanism.

Sci Total Environ 2021 Jul 21;797:149176. Epub 2021 Jul 21.

School of Geographic Sciences, East China Normal University, Shanghai 200241, China; Key Laboratory of Geographic Information Science (Ministry of Education), East China Normal University, Shanghai 200241, China; State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200241, China. Electronic address:

Intertidal wetlands provide important ecosystem functions by acting as nitrogen (N) cycling hotspots, which can reduce anthropogenic N loading from land to coastal waters. Benthic bioturbations are thought to play an important role in mediating N cycling in intertidal marshes. However, how the burrowing activity of benthos and their microbial symbionts affect N transformation and greenhouse gas nitrous oxide (NO) emission remains unclear in these environments. Here, we show that bioturbation of crabs reshaped the structure of intertidal microbial communities and their N cycling function. Molecular analyses suggested that the microbially-driven N cycling might be accelerated by crab bioturbation, as the abundances of most of the N related functional genes were higher on the burrow wall than those in the surrounding bulk sediments, except for genes involved in N fixation, dissimilatory nitrate reduction to ammonium (DNRA), and NO reduction, which were further confirmed by isotope-tracing experiments. Especially, the potential rates of the main NO production pathways, nitrification and denitrification, were 2-3 times higher in the burrow wall sediments. However, even higher NO emission rates (approximately 6 times higher) were observed in this unique microhabitat, which was due to a disproportionate increase in NO production over NO consumption driven by burrowing activity. In addition, the sources of NO were also significantly affected by crab bioturbation, which increased the contribution of hydroxylamine oxidation pathway. This study reveals the mechanism through which benthic bioturbations mediate N cycling and highlights the importance of considering burrowing activity when evaluating the ecological function of intertidal wetlands.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149176DOI Listing
July 2021

Complete chloroplast genome sequencing of five species and its application in the phylogeny and taxonomy of the genus.

Mitochondrial DNA B Resour 2021 15;6(8):2348-2352. Epub 2021 Jul 15.

National Willow Engineering Technology Research Center, Jiangsu Academy of Forestry, Nanjing, China.

In this study, whole chloroplast genomes of five species (, , , 'Hakuro Nishiki', and ) were sequenced. These chloroplast genomes were 155 ,605, 155, 763, 155, 552, 155, 538, and 155 ,550 bp in length, harboring 131 genes (77 unigenes), 37 tRNA genes, 8 rRNA genes, and 86 mRNA genes, respectively. The genes , , , , , , and were under positive selection among the 21 species. , atpF, and were under positive selection between the tree willow and shrub willow, and , , and were positively selected among the shrub genomes. The gene was most variable among the genomes. Phylogenetic analysis of 21 species and provide evidence that the cp genome data partially support the relationship with traditional taxonomic concepts in the Flora of China. This chloroplast genome elucidates taxonomy and provides evidence for evolutionary research.
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http://dx.doi.org/10.1080/23802359.2021.1950055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284152PMC
July 2021

Molecular mechanisms of opioid tolerance: From opioid receptors to inflammatory mediators (Review).

Exp Ther Med 2021 Sep 15;22(3):1004. Epub 2021 Jul 15.

Key Laboratory of Acupuncture and Neurology of Zhejiang Province, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China.

Opioids are considered the most effective analgesics for the treatment of both acute and chronic pain. However, prolonged opioid use can induce a certain level of tolerance to its analgesic effects, leading to a reduction in its effectiveness, addiction and abuse. A better understanding of the mechanisms underlying opioid tolerance may provide insights into this phenomenon and aid in the development of novel methods to combat the side effects of opioid tolerance. The present review focused on two major contributors to tolerance, opioid receptors and inflammatory mediators. The molecular mechanisms involved in the desensitization of the opioid receptors were briefly described, including their phosphorylation, internalisation and recycling. Subsequently, the effects of Toll like receptor 4/NOD-like receptor family pyrin domain containing 3-mediated proinflammatory responses in opioid tolerance were discussed, aiming in supporting the identification of novel therapeutic targets.
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http://dx.doi.org/10.3892/etm.2021.10437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311239PMC
September 2021

Ceftaroline Dosage Optimized for Pediatric Patients with Renal Impairment Using Physiologically Based Pharmacokinetic Modeling.

J Clin Pharmacol 2021 Jul 30. Epub 2021 Jul 30.

Department of Pharmacy, The First Affiliated Hospital of Fujian Medical University, 20 Cha Zhong M. Rd, Fuzhou, 350005, People's Republic of China.

Ceftaroline fosamil is a fifth-generation cephalosporin approved as a treatment for adults and children with community-acquired bacterial pneumonia (CABP) and acute bacterial skin and skin structure infections (ABSSSI). However, its pharmacokinetics has not been fully evaluated in children with renal impairment. This study aimed to propose proper ceftaroline dosages optimized for the renally impaired pediatric population using physiologically based pharmacokinetic (PBPK) modeling. A PBPK model of ceftaroline was established and verified to simulate its disposition in the healthy population and renally impaired adults and to predict the exposure in renally impaired pediatric patients. Consistency was confirmed between simulated and observed data after intravenous administration of various ceftaroline regimens; fold errors were within the two-fold error range. Among 6-year-old children, healthy subjects had 1.5-fold, 2-fold, and 2.6-fold lower AUCs than the moderate, severe, and end-stage renally impaired patient groups, respectively; among 1-year-old children, healthy subjects had 1.5-fold, 2.1-fold, and 2.5-fold lower AUCs than the respective renally impaired patient groups; among 1-month-old children, healthy subjects had 1.5-fold, 1.8-fold, and 2.2-fold lower AUCs than the respective renally impaired patient groups. The proposed dosage should be adjusted to 8, 6, and 5 mg/kg every 8 hours (q8h) for patients aged ≥ 2 years to < 18 years (≤ 33 kg) with moderate, severe, and end-stage renal impairment, respectively; 5, 4, and 3 mg/kg q8h for patients aged 2 months to < 2 years with moderate, severe, and end-stage renal impairment, respectively; 4, 3.5, and 2.5 mg/kg q8h for patients 0 to < 2 months of age with moderate, severe, and end-stage renal impairment, respectively. Furthermore, pharmacodynamic investigations demonstrated that adequate antimicrobial effects were attained at the proposed doses in three age groups. Hence, our PBPK model can be an effective tool to support ceftaroline dosage proposals for renally impaired pediatric patients. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jcph.1944DOI Listing
July 2021

Structural and functional basis for pan-CoV fusion inhibitors against SARS-CoV-2 and its variants with preclinical evaluation.

Signal Transduct Target Ther 2021 07 29;6(1):288. Epub 2021 Jul 29.

Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences and Biosafety Level 3 Laboratory, Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, China.

The COVID-19 pandemic poses a global threat to public health and economy. The continuously emerging SARS-CoV-2 variants present a major challenge to the development of antiviral agents and vaccines. In this study, we identified that EK1 and cholesterol-coupled derivative of EK1, EK1C4, as pan-CoV fusion inhibitors, exhibit potent antiviral activity against SARS-CoV-2 infection in both lung- and intestine-derived cell lines (Calu-3 and Caco2, respectively). They are also effective against infection of pseudotyped SARS-CoV-2 variants B.1.1.7 (Alpha) and B.1.1.248 (Gamma) as well as those with mutations in S protein, including N417T, E484K, N501Y, and D614G, which are common in South African and Brazilian variants. Crystal structure revealed that EK1 targets the HR1 domain in the SARS-CoV-2 S protein to block virus-cell fusion and provide mechanistic insights into its broad and effective antiviral activity. Nasal administration of EK1 peptides to hACE2 transgenic mice significantly reduced viral titers in lung and intestinal tissues. EK1 showed good safety profiles in various animal models, supporting further clinical development of EK1-based pan-CoV fusion inhibitors against SARS-CoV-2 and its variants.
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http://dx.doi.org/10.1038/s41392-021-00712-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8320318PMC
July 2021

Formation of Ultra-High Temperature Ceramic Hollow Microspheres as Promising Lightweight Thermal Insulation Materials via a Molten Salt-Assisted Template Method.

ACS Appl Mater Interfaces 2021 Jul 29. Epub 2021 Jul 29.

Science and Technology on Thermostructural Composite Materials Laboratory, Northwestern Polytechnical University, Xi'an 710072, China.

Ultra-high temperature ceramics (UHTCs) have become a vital candidate material system for thermal protection systems in aerospace applications. However, high thermal conductivity and high density are the main obstacles to the application of UHTCs. It is a promising solution to prepare porous UHTCs using UHTC hollow microspheres (HMs) as a pore-forming agent. In this work, UHTC (ZrC, TiC, and HfC) HMs are successfully synthesized using carbon hollow microspheres (CHMs) as a template to react with metal powders in molten salt. The diameter of ZrC HMs is about 1 μm and the wall thickness is about 100 nm. The density of each microsphere and the volume fraction of ZrC are 3.36 g/cm and 48.42 vol %, respectively. The morphology, microstructure, and phase composition of the obtained ZrC HMs were characterized. The formation mechanism of the UHTC HMs was discussed. Porous ZrC ceramics were prepared using ZrC HMs as a pore-forming agent. The density and thermal conductivity of the porous ZrC ceramics are 3.12 g/cm and 1.82 W/(m·K), respectively, which are 53.64 and 91.12% lower than the density and thermal conductivity of dense ZrC ceramics, respectively. The results indicated that ZrC HMs are promising as pore-forming agents or a matrix for lightweight thermal insulation and high-temperature resistance applications in ultra-high temperature environments.
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http://dx.doi.org/10.1021/acsami.1c09662DOI Listing
July 2021

Do cropping system and fertilization rate change water-stable aggregates associated carbon and nitrogen storage?

Environ Sci Pollut Res Int 2021 Jul 28. Epub 2021 Jul 28.

College of Agronomy and Biotechnology, China Agricultural University, Beijing, China.

Soil aggregates not only store carbon (C) and nitrogen (N) but hold a critical role in determining the nutrients supply, crop productivity, and climate change mitigation. However, the impact of cropping system and N fertilization on aggregate-associated C and N in both topsoil and subsoil remains unclear. Here, we assessed the effect of cropping systems (wheat-soybean vs. wheat-maize cropping systems) and N fertilization rates (0 N; medium N, 120 kg N ha; high N, 240 kg N ha) on soil water-stable aggregates distribution, as well as aggregate-associated C and N based on a field study in North China Plain. Our study suggests that the variations of soil organic carbon (SOC) and total nitrogen (TN) stocks were more affected by N fertilization than short-term cropping systems. In the wheat-soybean system, medium N increased the SOC stock by 19.18% and 15.73% as compared to high N in the topsoil and subsoil, respectively. Additionally, medium N resulted in 6.59-18.11% higher TN stock in the topsoil for both wheat-soybean and wheat-maize cropping systems as compared to 0 N and high N. Notably, the water-stable macroaggregates (> 0.25 mm) in the topsoil occupied more than 70% of the soil, which increased under medium N in the wheat-soybean cropping system. In conclusion, medium N fertilization combined with a legume-based cropping could be used to improve SOC stock, promote soil aggregation, and enhance aggregate-associated C.
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http://dx.doi.org/10.1007/s11356-021-15562-2DOI Listing
July 2021

Elevated Ras related GTP binding B (RRAGB) expression predicts poor overall survival and constructs a prognostic nomogram for colon adenocarcinoma.

Bioengineered 2021 Dec;12(1):4620-4632

Department of General Surgery, Taizhou Hospital of Traditional Chinese Medicine, Taizhou, Jiangsu Province, China.

Currently, no articles have explored the roles of RRAGB gene in the occurrence and development of cancer. By means of The Cancer Genome Atlas (TCGA) data mining, we found that this gene might be a novel prognostic predictor for colon adenocarcinoma (COAD). Hence, this article was carried out to explore its roles in COAD and associations with immunity. RRAGB single-gene expression matrix and corresponding clinical information were extracted from TCGA database. Univariate/multivariate cox regression analyses and gene set enrichment analysis (GSEA) were utilized to identify independent prognostic factors and RRAGB related pathways, respectively. Relationships between RRAGB and immunity were also analyzed. Boxplot and K-M survival analysis indicated that RRAGB was not only differently expressed in COAD ( < 0.05), but also significantly associated with overall survival (OS; < 0.05). Univariate and multivariate Cox hazard regression analyses indicated that RRAGB could serve as an independent prognostic factor for COAD (both < 0.05). GSEA identified five signaling pathways significantly enriched in the high-RRAGB expression phenotype. Moreover, a RRAGB-based nomogram was successfully constructed and displayed a satisfactory performance. In addition, RRAGB expression was found to be significantly associated with microsatellite instability (MSI), tumor mutational burden (TMB) and immunity. Our results revealed that RRAGB could be a prognostic biomarker for COAD in terms of OS and markedly related to MSI, TMB, and immunity. We also constructed an RRAGB-based nomogram with a satisfactory performance. Further researches should be carried out to validate our findings.
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http://dx.doi.org/10.1080/21655979.2021.1956402DOI Listing
December 2021

Genome-wide identification of candidate aquaporins involved in water accumulation of pomegranate outer seed coat.

PeerJ 2021 15;9:e11810. Epub 2021 Jul 15.

College of Resource and Environment, Anhui Science and Technology University, Fengyang, China.

Aquaporins (AQPs) are a class of highly conserved integral membrane proteins that facilitate the uptake and transport of water and other small molecules across cell membranes. However, little is known about AQP genes in pomegranate ( L.) and their potential role in water accumulation of the outer seed coat. We identified 38 PgrAQP genes in the pomegranate genome and divided them into five subfamilies based on a comparative analysis. Purifying selection played a role in the evolution of PgrAQP genes and a whole-genome duplication event in Myrtales may have contributed to the expansion of PgrTIP, PgrSIP, and PgrXIP genes. Transcriptome data analysis revealed that the PgrAQP genes exhibited different tissue-specific expression patterns. Among them, the transcript abundance of were significantly higher than that of other subfamilies. The mRNA transcription levels of , , and showed a significant linear relationship with water accumulation in seed coats, indicating that PgrPIP1.3/PgrPIP2.8 located in the plasma membrane and PgrSIP1.2 proteins located on the tonoplast may be involved in water accumulation and contribute to the cell expansion of the outer seed coat, which then develops into juicy edible flesh. Overall, our results provided not only information on the characteristics and evolution of PgrAQPs, but also insights on the genetic improvement of outer seed coats.
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http://dx.doi.org/10.7717/peerj.11810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286702PMC
July 2021

Compact Color Biofinder (CoCoBi): Fast, Standoff, Sensitive Detection of Biomolecules and Polyaromatic Hydrocarbons for the Detection of Life.

Appl Spectrosc 2021 Jul 26:37028211033911. Epub 2021 Jul 26.

NASA Langley Research Center, Hampton, VA, USA.

We have developed a compact instrument called the "COmpact COlor BIofinder", or CoCoBi, for the standoff detection of biological materials and organics with polyaromatic hydrocarbons (PAHs) using a nondestructive approach in a wide area. The CoCoBi system uses a compact solid state, conductively cooled neodymium-doped yttrium aluminum garnet (Nd:YAG) nanosecond pulsed laser capable of simultaneously providing two excitation wavelengths, 355 and 532 nm, and a compact, sensitive-gated color complementary metal-oxide-semiconductor camera detector. The system is compact, portable, and determines the location of biological materials and organics with PAHs in an area 1590 cm wide, from a target distance of 3 m through live video using fast fluorescence signals. The CoCoBi system is highly sensitive and capable of detecting a PAH concentration below 1 part per billion from a distance of 1 m. The color images provide the simultaneous detection of various objects in the target area using shades of color and morphological features. We demonstrate that this unique feature successfully detected the biological remains present in a 150-million-year-old fossil buried in a fluorescent clay matrix. The CoCoBi was also successfully field-tested in Hawaiian ocean water during daylight hours for the detection of natural biological materials present in the ocean. The wide-area and video-speed imaging capabilities of CoCoBi for biodetection may be highly useful in future NASA rover-lander life detection missions.
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http://dx.doi.org/10.1177/00037028211033911DOI Listing
July 2021

Clinical efficacy and safety of different doses of low molecular weight heparin in the treatment of preeclampsia with fetal growth restriction.

Authors:
Gong Chen Jie Zhou

Minerva Pediatr (Torino) 2021 Jul 26. Epub 2021 Jul 26.

Department of Obstetrics, Huaian Maternal and Child Health Hospital, Huaian, China -

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http://dx.doi.org/10.23736/S2724-5276.21.06514-9DOI Listing
July 2021

Electroacupuncture alleviates the transition from acute to chronic pain through the p38 MAPK/TNF-α signalling pathway in the spinal dorsal horn.

Acupunct Med 2021 Jul 24:9645284211020766. Epub 2021 Jul 24.

Department of Acupuncture and Rehabilitation, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing City, China.

Background: Hyperalgesic priming (HP) is a model of the transition from acute to chronic pain. Electroacupuncture (EA) could inhibit pain development through the peripheral dorsal root ganglia; however, it is unclear whether it can mitigate the transition from acute to chronic pain by attenuating protein expression in the p38 MAPK (mitogen-activated protein kinase)/tumour necrosis factor alpha (TNF-α) pathway in the spinal dorsal horn.

Aims: We aimed to determine whether EA could prevent the transition from acute to chronic pain by affecting the p38 MAPK/TNF-α pathway in the spinal dorsal horn in a rat model established using HP.

Methods: We first randomly subdivided 30 male Sprague-Dawley (SD) rats into 5 groups ( = 6 per group): control (N), sham HP (Sham-HP), HP, HP + SB203580p38 MAPK (HP+SB203580), and HP + Lenalidomide (CC-5013) (HP+Lenalidomide). We then randomly subdivided a further 30 male SD rats into 5 groups ( = 6 per group): Sham-HP, HP, sham EA (Sham EA), EA (EA), and EA + U-46619 p38 MAPK agonist (EA+U-46619). We assessed the effects of EA on the mechanical paw withdrawal threshold and p38 MAPK/TNF-α expression in the spinal dorsal horn of rats subjected to chronic inflammatory pain.

Results: Rats in the EA group had reduced p38 MAPK and TNF-α expression and had significantly reduced mechanical hyperalgesia compared with rats in the other groups.

Conclusion: Our findings indicate that EA could increase the mechanical pain threshold in rats and inhibit the transition from acute pain to chronic pain. This mechanism could involve reduced p38 MAPK/TNF-α expression in the spinal dorsal horn.
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http://dx.doi.org/10.1177/09645284211020766DOI Listing
July 2021

Clinical efficacy of jade wind-barrier powder combined with loratadine in the treatment of pediatric allergic rhinitis.

Am J Transl Res 2021 15;13(6):6516-6524. Epub 2021 Jun 15.

Department of Traditional Chinese Medicine, The Affiliated Hospital of Qingdao University Qingdao, Shandong Province, China.

Objective: To investigate the clinical efficacy of Jade Wind-Barrier Powder combined with Loratadine in the treatment of pediatric allergic rhinitis (PAR).

Methods: The clinical data of 101 children with allergic rhinitis (AR) admitted to the Affiliated Hospital of Qingdao University and Affiliated Hospital of Nanjing University of Traditional Chinese Medicine from January 2019 to December 2019 were retrospectively analyzed. The children were randomly divided into Group A (n=50) and Group B (n=51) in accordance with a random number table. Group A was treated with Loratadine only, while Group B was treated with Loratadine combined with Jade Wind-Barrier powder. The clinical efficacy, symptom disappearance time, symptom scores before and after treatment, indices of immunological function, changes in the indices of inflammatory factors and disease recurrence were compared between the two groups.

Results: The overall response rate (ORR) in Group B (96.08%) was higher than that in Group A (76.00%) ( < 0.05). The disappearance time of sneezing, stuffy nose, runny nose and itchy nose in Group B was shorter than that in Group A ( < 0.05). After treatment, Group B exhibited lower symptom scores for sneezing, stuffy nose, runny nose and itchy nose, lower levels of IL-13, IL-4 and TNF-α, and higher CD4CD25, CD19 and CD8 than Group A ( < 0.05). The recurrence rate in Group B (3.92%) was lower than that in Group A (26.00%) ( < 0.05).

Conclusion: Jade Wind-Barrier powder combined with Loratadine can improve clinical symptoms, immunity, inflammation levels and disease recurrence rate of PAR patients, with a significant clinical efficacy.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290791PMC
June 2021

Comparative effect of electroacupuncture with different frequency on headache attacks in migraine outpatients: study protocol for a randomised placebo-controlled trial.

Trials 2021 Jul 23;22(1):483. Epub 2021 Jul 23.

Department of Acupuncture, The Third Affiliated Hospital of Zhejiang Chinese Medical University, No. 219 Moganshan Road, Xihu District, Hangzhou, 310005, Zhejiang Province, China.

Background: Headache attacks severely impaired life quality and increase the economic burden of migraineurs. Electroacupuncture (EA) has been used worldwidely to treat several pain-related diseases including migraines. However, whether EA with low or high frequency exerts a distinct analgesic effect remains unknown and needs further study.

Methods/design: This study is a randomised, single-blinded, placebo-controlled trial with three parallel arms. A total of 144 migraine outpatients will be randomly allocated to the 2 Hz EA group, 100 Hz EA group and placebo control group. The duration of the trial is 20 weeks, including a 4-week-long baseline assessment period (weeks - 4-0), a 4-week-long treatment period (weeks 1-4) and a 12-week-long follow-up period (weeks 5-16). Twelve treatment sessions will be performed over a 4-week period (weeks 1-4). The primary outcome will be measured by the frequency of migraine attacks in the past 4 weeks at the end of week 4 post-randomisation. The secondary outcome will be measured by the frequency of migraine attacks in the past 4 weeks at the end of weeks 8, 12 and16 post-randomisation; number of days with migraine; dosage of ibuprofen; the scores of visual analogue scale (VAS); Self-Rating Anxiety Scale (SAS); Self-Rating Depression Scale (SDS); and Migraine Specific Quality of Life questionnaire (MSQ) in the past 4 weeks at the end of weeks 4, 8, 12 and 16 post-randomisation. Safety assessment, compliance and blinding evaluation will be carried out at the end of week 16 post-randomisation.

Discussion: The recruitment will be started on 1 June 2021 and expected to finish on 31 May 2023. We aimed to clarify the dominant frequency of EA on headache attacks in a migraineur.

Trial Registration: Chinese Clinical Trial Registry ChiCTR-1800017259 . Registered on 20 July 2018.
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http://dx.doi.org/10.1186/s13063-021-05429-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299449PMC
July 2021

Corrigendum to 'Dezocine attenuates the remifentanil-induced postoperative hyperalgesia by inhibition of phosphorylation of CaMKⅡα' [Eur J Pharmacol. 2020 Feb 15; 869: 172882].

Eur J Pharmacol 2021 Jul 20;908:174359. Epub 2021 Jul 20.

Department of Anesthesiology, Affiliated Hospital of Guilin Medical University, Gulin, Guangxi, 541004, China; Department of Anesthesiology, Wuhan NO. 1 Hospital, Wuhan, Hubei, 430022, China. Electronic address:

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http://dx.doi.org/10.1016/j.ejphar.2021.174359DOI Listing
July 2021

A combined GWAS approach reveals key loci for socially-affected traits in Yorkshire pigs.

Commun Biol 2021 Jul 20;4(1):891. Epub 2021 Jul 20.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, China.

Socially affected traits in pigs are controlled by direct genetic effects and social genetic effects, which can make elucidation of their genetic architecture challenging. We evaluated the genetic basis of direct genetic effects and social genetic effects by combining single-locus and haplotype-based GWAS on imputed whole-genome sequences. Nineteen SNPs and 25 haplotype loci are identified for direct genetic effects on four traits: average daily feed intake, average daily gain, days to 100 kg and time in feeder per day. Nineteen SNPs and 11 haplotype loci are identified for social genetic effects on average daily feed intake, average daily gain, days to 100 kg and feeding speed. Two significant SNPs from single-locus GWAS (SSC6:18,635,874 and SSC6:18,635,895) are shared by a significant haplotype locus with haplotype alleles 'GGG' for both direct genetic effects and social genetic effects in average daily feed intake. A candidate gene, MT3, which is involved in growth, nervous, and immune processes, is identified. We demonstrate the genetic differences between direct genetic effects and social genetic effects and provide an anchor for investigating the genetic architecture underlying direct genetic effects and social genetic effects on socially affected traits in pigs.
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http://dx.doi.org/10.1038/s42003-021-02416-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292486PMC
July 2021

[Corrigendum] Integrated transcriptomic and epigenetic data analysis identifies aberrant expression of genes in acute myeloid leukemia with MLL‑AF9 translocation.

Mol Med Rep 2021 Sep 19;24(3). Epub 2021 Jul 19.

Department of Hematology, Tongji Hospital, Tongji University School of Medicine, Shanghai 200092, P.R. China.

Following the publication of this paper, the authors have realized that the final article did not indicate in the Authors' Contribution section that Fangce Wang and Zheng Li made equal contributions to this work (FW and ZL performed most of the statistical analyses and drafted the initial version of the manuscript). Therefore, the affiliations for this paper should have been written as follows (changes are highlighted in bold): FANGCE WANG, ZHENG LI, GUANGMING WANG, XIAOXUE TIAN, JIE ZHOU, WENLEI YU, ZHUOYI FAN, LIN DONG, JINYUAN LU, JUN XU, WENJUN ZHANG and AIBIN LIANG Department of Hematology, Tongji Hospital, Tongji University School of Medicine, Shanghai 200092; Medical Center for Stem Cell Engineering and Transformation, East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China. Contributed equally. The authors confirm that there are no further errors in the paper, and all the authors agree to this correction. The authors and the Editor apologize for any inconvenience caused. [the original article was published in 21: 883‑893, 2020, DOI: 10.3892/mmr.2019.10849].
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http://dx.doi.org/10.3892/mmr.2021.12295DOI Listing
September 2021

Delivery of acetamiprid to tea leaves enabled by porous silica nanoparticles: efficiency, distribution and metabolism of acetamiprid in tea plants.

BMC Plant Biol 2021 Jul 16;21(1):337. Epub 2021 Jul 16.

State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Agricultural University, Hefei, 230036, China.

Background: Pesticide residue and its poor utilization remains problematic in agricultural development. To address the issue, a nano-pesticide has been developed by incorporating pesticide acetamiprid in porous silica nanoparticles.

Results: This nano-pesticide had an acetamiprid loading content of 354.01 mg g. Testing LC value against tea aphids of the commercial preparation was three times that of the nano-pesticide. In tea seedlings (Camellia sinensis L.), acetamiprid was transported upward from the stem to the young leaves. On day 30, the average retained concentrations in tea leaves treated with the commercial preparation were about 1.3 times of that in the nano-pesticide preparation. The residual concentrations of dimethyl-acetamiprid in leaves for plants treated with the commercial preparation were about 1.1 times of that in the nano-pesticide preparation. Untargeted metabolomics of by LC-MS on the young leaves of tea seedlings under nano-pesticide and commercial pesticide treatments showed significant numbers of differentially expressed metabolites (P < 0.05 and VIP > 1). Between the nano-pesticide treatment group and the commercial preparation treatment group there were 196 differentially expressed metabolites 2 h after treatment, 200 (7 day), 207 (21 day), and 201 (30 day) in negative ion mode, and 294 (2 h), 356 (7 day), and 286 (30 day) in positive ion mode. Preliminary identification showed that the major differentially expressed metabolites were glutamic acid, salicylic acid, p-coumaric acid, ribonic acid, glutamine, naringenin diglucoside, sanguiin H4, PG (34:2) and epiafzelechin.

Conclusions: This work demonstrated that our nano-pesticide outperformed the conventional pesticide acetamiprid in terms of insecticidal activity and pesticide residue, and the absorption, transportation and metabolism of nano-pesticide in tea plant were different, which pave a new pathway for pest control in agricultural sector.
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http://dx.doi.org/10.1186/s12870-021-03120-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283891PMC
July 2021

Virulence and community dynamics of fungal species with vertical and horizontal transmission on a plant with multiple infections.

PLoS Pathog 2021 Jul 15;17(7):e1009769. Epub 2021 Jul 15.

State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, China.

The virulence evolution of multiple infections of parasites from the same species has been modeled widely in evolution theory. However, experimental studies on this topic remain scarce, particularly regarding multiple infections by different parasite species. Here, we characterized the virulence and community dynamics of fungal pathogens on the invasive plant Ageratina adenophora to verify the predictions made by the model. We observed that A. adenophora was highly susceptible to diverse foliar pathogens with mixed vertical and horizontal transmission within leaf spots. The transmission mode mainly determined the pathogen community structure at the leaf spot level. Over time, the pathogen community within a leaf spot showed decreased Shannon diversity; moreover, the vertically transmitted pathogens exhibited decreased virulence to the host A. adenophora, but the horizontally transmitted pathogens exhibited increased virulence to the host. Our results demonstrate that the predictions of classical models for the virulence evolution of multiple infections are still valid in a complex realistic environment and highlight the impact of transmission mode on disease epidemics of foliar fungal pathogens. We also propose that seedborne fungi play an important role in structuring the foliar pathogen community from multiple infections within a leaf spot.
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http://dx.doi.org/10.1371/journal.ppat.1009769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315517PMC
July 2021

Inhibition of Nuclear Factor Kappa B as a Therapeutic Target for Lung Cancer.

Altern Ther Health Med 2021 Jul 16. Epub 2021 Jul 16.

Context: A wide variety of malignancies common to humans display abnormal constitutive expression of nuclear factor kappa beta (NF-κβ). The study of NF-κβ can increase understanding of its role in cancer, especially in lung-tumor formation.

Objective: This review intended to examine the research on the impact of the NF-κβ signaling pathway on the development of malignancies of the lungs and the advances in the regulation of that pathway to mitigate and provide treatment for lung carcinoma.

Design: The research team performed a narrative review by searching the PubMed, EMBASE, and Web of Science databases. The search used the keywords NF-κB or NF-kappa B or nuclear factor kappa B and non-small-cell lung cancer or non-small-cell lung carcinoma or NSCLC or lung cancer or lung adenocarcinoma or lung squamous carcinoma.

Setting: This study were conducted at Department of Respiratory and Critical Care Medicine, Qingxian People's Hospital, Cangzhou City, Hebei Province, People's Republic of China.

Results: As a major cell survival signal, nuclear factor-kappaB (NF-kappaB) is involved in multiple steps in carcinogenesis and in cancer cell's resistance to chemo- and radio-therapy. Recent studies with animal models and cell culture systems have established the links between NF-kappaB and lung carcinogenesis, highlighting the significance of targeting NF-kappa signaling pathway for lung cancer treatment and chemoprevention.

Conclusions: While no convincing evidence exists that survival of lung cancer cells is dependent on NF-κβ, inhibition of NF-κβ is a potent supporting therapy for enhancing the therapeutic effects of chemotherapy and radiotherapy. Constitutive and therapeutically induced activation of NF-κβ reduces the tumor-massacre effect of treatment, so inhibiting NF-κβ might increase antitumor activity.
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July 2021

Optimized Projection and Fisher Discriminative Dictionary Learning for EEG Emotion Recognition.

Front Psychol 2021 28;12:705528. Epub 2021 Jun 28.

School of Computer Science and Artificial Intelligence, Changzhou University, Changzhou, China.

Electroencephalogram (EEG)-based emotion recognition (ER) has drawn increasing attention in the brain-computer interface (BCI) due to its great potentials in human-machine interaction applications. According to the characteristics of rhythms, EEG signals usually can be divided into several different frequency bands. Most existing methods concatenate multiple frequency band features together and treat them as a single feature vector. However, it is often difficult to utilize band-specific information in this way. In this study, an optimized projection and Fisher discriminative dictionary learning (OPFDDL) model is proposed to efficiently exploit the specific discriminative information of each frequency band. Using subspace projection technology, EEG signals of all frequency bands are projected into a subspace. The shared dictionary is learned in the projection subspace such that the specific discriminative information of each frequency band can be utilized efficiently, and simultaneously, the shared discriminative information among multiple bands can be preserved. In particular, the Fisher discrimination criterion is imposed on the atoms to minimize within-class sparse reconstruction error and maximize between-class sparse reconstruction error. Then, an alternating optimization algorithm is developed to obtain the optimal solution for the projection matrix and the dictionary. Experimental results on two EEG-based ER datasets show that this model can achieve remarkable results and demonstrate its effectiveness.
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http://dx.doi.org/10.3389/fpsyg.2021.705528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274488PMC
June 2021

Sleep disturbance mediates the relationship between depressive symptoms and cognitive function in older adults with mild cognitive impairment.

Geriatr Nurs 2021 Jul 10;42(5):1019-1023. Epub 2021 Jul 10.

Shenzhen Qianhai Shekou Free Trade Zone Hospital, No.36, 7th Industrial Road, Nanshan District, Shenzhen City, Guangdong province, 518067, China. Electronic address:

This study examined whether and to what extent sleep disturbance mediates the effects of depressive symptoms on the cognition of individuals with mild cognitive impairment (MCI), who represent a high-risk group for developing dementia. Cross-sectional data were obtained from a sample of 204 Chinese community-dwelling older adults with MCI. MCI subjects were screened using the Montreal Cognitive Assessment, sleep quality was measured using the Pittsburgh Sleep Quality Index, and depressive symptoms were assessed using the Geriatric Depression Scale. Mediation analysis was conducted using the PROCESS macro with 10,000 bootstrap samples. The significant mediating effect of sleep quality on the association between depressive symptoms and cognition (Beta = -0.025; 95% CI, -0.054 to -0.007) explains 26% of the total effect of depressive symptoms on cognition and implies that the timely detection and management of sleep disturbance among the MCI population is highly important, especially for those with depressive symptoms.
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http://dx.doi.org/10.1016/j.gerinurse.2021.06.004DOI Listing
July 2021

IL-6/STAT3 signaling activation exacerbates high fructose-induced podocyte hypertrophy by ketohexokinase-A-mediated tristetraprolin down-regulation.

Cell Signal 2021 Jul 10;86:110082. Epub 2021 Jul 10.

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, PR China. Electronic address:

Glomerular hypertrophy is a crucial factor of severe podocyte damage and proteinuria. Our previous study showed that high fructose induced podocyte injury. The current study aimed to explore a novel molecular mechanism underlying podocyte hypertrophy induced by high fructose. Here we demonstrated for the first time that high fructose significantly initiated the hypertrophy in rat glomeruli and differentiated human podocytes (HPCs). Consistently, it induced inflammatory response with the down-regulation of anti-inflammatory factor zinc-finger protein tristetraprolin (TTP) and the activation of interleukin-6 (IL-6)/signal transducer and activator of transcription 3 (STAT3) signaling in these animal and cell models. Subsequently, high-expression of microRNA-92a-3p (miR-92a-3p) and its target protein cyclin-dependent kinase inhibitor p57 (P57) down-regulation, representing abnormal proliferation and apoptosis, were observed in vivo and in vitro. Moreover, high fructose increased ketohexokinase-A (KHK-A) expression in rat glomeruli and differentiated HPCs. Exogenous IL-6 stimulation up-regulated IL-6/STAT3 signaling and miR-92a-3p, reduced P57 expression and promoted podocyte proliferation, apoptosis and hypertrophy in vitro. The data from anti-inflammatory agent maslinic acid treatment or TTP siRNA transfection showed that high fructose may decrease TTP to activate IL-6/STAT3 signaling in podocyte overproliferation and apoptosis, causing podocyte hypertrophy. Whereas, KHK-A siRNA transfection remarkably restored high fructose-induced TTP down-regulation, IL-6/STAT3 signaling activation, podocyte overproliferation, apoptosis and hypertrophy in differentiated HPCs. Taken together, these results suggested that high fructose possibly increased KHK-A expression to down-regulate TTP, subsequently activated IL-6/STAT3 signaling to interfere with podocyte proliferation and apoptosis by up-regulating miR-92a-3p to suppress P57 expression, causing podocyte hypertrophy. Therefore, the inactivation of IL-6/STAT3 to relieve podocyte hypertrophy mediated by inhibiting KHK-A to increase TTP may be a novel strategy for high fructose diet-associated podocyte injury and proteinuria.
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http://dx.doi.org/10.1016/j.cellsig.2021.110082DOI Listing
July 2021

Rice Root Hair Phenotypes Imaged by Cryo-SEM.

Bio Protoc 2021 Jun 5;11(11):e4037. Epub 2021 Jun 5.

Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.

Cryo-scanning electron microscopy (cryo-SEM) was first introduced for scientific use in the 1980s. Since then, cryo-SEM has become a routine technique for studying the surfaces and internal structures of biological samples with a high water content. In contrast to traditional SEM, cryo-SEM requires no sample pretreatment processes; thus, we can obtain the most authentic images of the sample shape and structure. Cryo-SEM has two main steps: cryoprocessing of samples and scanning electron microscopy (SEM) observation. The cryoprocessing step includes preparation of the cooled slushing station, cooling of the preparation chamber, sample preparation, and sputtering. The sample is then transferred to an SEM cold stage for observation. We used cryo-SEM to study rice root hair tissues, but the methods and protocols can be applied to other root systems. This protocol optimizes the two key operation steps of reducing the humidity in the growth chamber and previewing the samples before sputtering and can more quickly obtain high-quality images.
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http://dx.doi.org/10.21769/BioProtoc.4037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249913PMC
June 2021

Diagnostic accuracy of cardiac magnetic resonance tissue tracking technology for differentiating between acute and chronic myocardial infarction.

Quant Imaging Med Surg 2021 Jul;11(7):3070-3081

Department of Radiology, The First Affiliated Hospital of China Medical University, Shenyang, China.

Background: This study aimed to explore the diagnostic accuracy of cardiac magnetic resonance tissue tracking (CMR-TT) technology in the quantitative evaluation of left myocardial infarction for differentiating between acute and chronic myocardial infarction.

Methods: A total of 104 human subjects were enrolled in this prospective study. Among them, 64 healthy subjects and 40 patients with left ventricular myocardial infarction and 7 days and 6 months' follow-up CMR studies, including steady-state free precession (SSFP) sequence and late gadolinium enhancement MR imaging, were enrolled. The strain parameters of the infarcted myocardium, its corresponding remote segments, and global right ventricular strain were analyzed using tissue tracking technology, and CMR-TT 3D strain parameters in radial, circumferential, and longitudinal directions were obtained. Receiver operating characteristic (ROC) analysis was used to determine the diagnostic accuracy of the CMR-TT strain parameters for discriminating between acute and chronic myocardial infarction.

Results: Peak radial strain (RS) of infarcted myocardium increased from 12.99±7.28 to 18.57±6.66 at 6 months (P<0.001), whereas peak circumferential strain (CS) increased from -8.82±4.71 to -12.78±3.55 (P<0.001). CS yielded the best areas under the ROC curve (AUC) of 0.751 in showing differentiation between acute and chronic myocardial infarction of all the strain parameters obtained. The highest significant differences between acute myocardial infarction and normal myocardium, both in the left and right ventricles, were also found in the RS (P<0.001) and CS (P<0.001).

Conclusions: RS and CS obtained by CMR-TT have high sensitivity and specificity in the differential diagnosis of acute versus chronic myocardial infarction, and their use is thus worth popularizing in clinical application.
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http://dx.doi.org/10.21037/qims-20-1109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250004PMC
July 2021

MicroRNA-Mediated Responses to Chromium Stress Provide Insight Into Tolerance Characteristics of .

Front Plant Sci 2021 23;12:666117. Epub 2021 Jun 23.

Department of Forage Science, College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, China.

Chromium (Cr) is a heavy metal in nature, which poses a potential risk to toxicity to both animals and plants when releasing into the environment. However, the regulation of microRNA (miRNA)-mediated response to heavy metal Cr has not been studied in . In this study, based on high-throughput miRNA sequencing, a total of 104 conserved miRNAs and 158 nonconserved miRNAs were identified. Among them, there were 45 differentially expressed miRNAs in roots and 13 differentially expressed miRNAs in leaves. The hierarchical clustering analysis showed that these miRNAs were preferentially expressed in a certain tissue. There were 833 differentially expressed target genes of 45 miRNAs in roots and 280 differentially expressed target genes of 13 miRNA in leaves. After expression trend analysis, five significantly enriched modules were obtained in roots, and three significantly enriched trend blocks in leaves. Based on the candidate gene annotation and gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) function analysis, miR167a, novel_miR15, and novel_miR22 and their targets were potentially involved in Cr transportation and chelation. Besides, miR156a, miR164, miR396d, and novel_miR155 were identified as participating in the physiological and biochemical metabolisms and the detoxification of Cr of plants. The results demonstrated the critical role of miRNA-mediated responses to Cr treatment in , which involves ion uptake, transport, accumulation, and tolerance characteristics.
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http://dx.doi.org/10.3389/fpls.2021.666117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261058PMC
June 2021

Relationship between the difference in electric pulp test values and the diagnostic type of pulpitis.

BMC Oral Health 2021 07 10;21(1):339. Epub 2021 Jul 10.

Department of Endodontics, School of Stomatology, Tianjin Medical University, #12 Qi Xiang Tai Road, He Ping District, Tianjin, 300070, China.

Background: According to the diagnosis criteria of the American Association of Endodontists (AAE), sensitive responses to cold and/or heat tests of suspected teeth compared with those of control teeth can be used for the diagnosis of pulpitis, but the role of electric pulp test (EPT) is not mentioned. It is believed that EPT has some limitations in determining the vitality of the pulp. The aim of this study was to explore the association between the difference in EPT values and the differential diagnoses of reversible pulpitis (RP) and symptomatic irreversible pulpitis (SIRP) caused by dental caries.

Methods: A total of 203 cases with pulpitis caused by dental caries were included. A diagnosis of pulpitis was made on the basis of the diagnostic criteria of AAE. Patient demographic and clinical examination data were collected. The EPT values of the suspected teeth and control teeth were measured, and the differences between them were calculated. The correlation between the difference in the EPT values and diagnosis of pulpitis was analyzed using univariate and multivariate logistic regression.

Results: In the 203 cases (78 males and 125 females; 115 cases of RP, 88 cases of SIRP; 9 anterior teeth, 59 premolars, and 135 molars), the mean patient age was 34.04 ± 13.02 (standard deviation) years. The unadjusted (crude) model, model 1 (adjusted for age), model 2 (adjusted for age and sex), and model 3 (adjusted for age, sex, and tooth type) were established for the statistical analyses. In model 3 [odds ratio (OR) = 1.025; 95% confidence interval (CI) 1.002-1.050; P = 0.035], the difference in EPT values between RP and SIRP was statistically significant. However, the areas under the curve of predictive probability of the crude model, model 1, model 2, and model 3 were 0.565, 0.570, 0.585, and 0.617, respectively, showing that the model accuracy was low. The P-value for the trend in differences between the EPT values as a categorical variable showed that the differences in the EPT values, comparing RP and SIRP, were not statistically significant.

Conclusions: Based on the present data, the difference in EPT values was not sufficient to differentiate RP from SIRP.
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http://dx.doi.org/10.1186/s12903-021-01696-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272274PMC
July 2021

Delineating spatiotemporal and hierarchical development of human fetal innate lymphoid cells.

Cell Res 2021 Jul 8. Epub 2021 Jul 8.

State Key Laboratory of Proteomics, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing, China.

Whereas the critical roles of innate lymphoid cells (ILCs) in adult are increasingly appreciated, their developmental hierarchy in early human fetus remains largely elusive. In this study, we sorted human hematopoietic stem/progenitor cells, lymphoid progenitors, putative ILC progenitor/precursors and mature ILCs in the fetal hematopoietic, lymphoid and non-lymphoid tissues, from 8 to 12 post-conception weeks, for single-cell RNA-sequencing, followed by computational analysis and functional validation at bulk and single-cell levels. We delineated the early phase of ILC lineage commitment from hematopoietic stem/progenitor cells, which mainly occurred in fetal liver and intestine. We further unveiled interleukin-3 receptor as a surface marker for the lymphoid progenitors in fetal liver with T, B, ILC and myeloid potentials, while IL-3RA lymphoid progenitors were predominantly B-lineage committed. Notably, we determined the heterogeneity and tissue distribution of each ILC subpopulation, revealing the proliferating characteristics shared by the precursors of each ILC subtype. Additionally, a novel unconventional ILC2 subpopulation (CRTH2 CCR9 ILC2) was identified in fetal thymus. Taken together, our study illuminates the precise cellular and molecular features underlying the stepwise formation of human fetal ILC hierarchy with remarkable spatiotemporal heterogeneity.
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http://dx.doi.org/10.1038/s41422-021-00529-2DOI Listing
July 2021

Fructose drives mitochondrial metabolic reprogramming in podocytes via Hmgcs2-stimulated fatty acid degradation.

Signal Transduct Target Ther 2021 Jul 9;6(1):253. Epub 2021 Jul 9.

The State Key Laboratory of Pharmaceutical Biotechnology, Medical School, School of Life Sciences, Nanjing University, Nanjing, China.

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http://dx.doi.org/10.1038/s41392-021-00570-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266798PMC
July 2021

Investigation of pesticide residue removal effect of gelatinized starch using surface-enhanced Raman scattering mapping.

Food Chem 2021 Jun 24;365:130448. Epub 2021 Jun 24.

The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors and Department of Chemistry, Shanghai Normal University, Shanghai 200234, PR China. Electronic address:

This study investigated the effectiveness of gelatinized starch (GS) in the removal of surface and internalized pesticide residues from basil leaves. Surface activity of GS was confirmed by surface tension and fluorescence study. Surface-enhanced Raman scattering (SERS) mapping was applied for in situ and real-time tracking of pesticides over time. Results showed that gelatinized starch has better and safer pesticide removing effect compared to commercial surfactants. Simulation study showed that starch fragment can adjust its three-dimensional conformation according to the size of the guest with ~ four repeating α-1,4-d-glucopyranose residues interacting efficiently with pesticides. However, washing by small molecular weight surfactants will lead to a "secondary contamination" due to its amphilphilic nature and small molecular size, which can escort pesticide deeper into the leaf. Due to the wide availability, easy fabrication, efficient rinsing effect and bio-safety nature, GS should be highly recommended in family practice.
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http://dx.doi.org/10.1016/j.foodchem.2021.130448DOI Listing
June 2021
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