Publications by authors named "Jie Zhou"

2,696 Publications

  • Page 1 of 1

Guidelines for Diagnosis and Treatment of Hepatocellular Carcinoma with Portal Vein Tumor Thrombus in China (2021 Edition).

Liver Cancer 2022 Jul 17;11(4):315-328. Epub 2022 Mar 17.

Department of Radiation Oncology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China.

Portal vein tumor thrombus (PVTT) is very common and it plays a major role in the prognosis and clinical staging of hepatocellular carcinoma (HCC). We have published the first version of the guideline in 2016 and revised in 2018. Over the past several years, many new evidences for the treatment of PVTT become available, especially for the advent of new targeted drugs and immune checkpoint inhibitors which have further improved the prognosis of PVTT. So, the Chinese Association of Liver Cancer and Chinese Medical Doctor Association revised the 2018 version of the guideline to adapt to the development of PVTT treatment. Future treatment strategies for HCC with PVTT in China would depend on new evidences from more future clinical trials.
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http://dx.doi.org/10.1159/000523997DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294940PMC
July 2022

Government environmental protection subsidies, environmental tax collection, and green innovation: evidence from listed enterprises in China.

Environ Sci Pollut Res Int 2022 Aug 16. Epub 2022 Aug 16.

Lianyungang Normal College, Jiangsu, 222000, Lianyungang, China.

The government has issued a series of environmental laws and regulations to solve ecological problems, regulate environmental pollution, and drive enterprises to carry out green innovations. This paper constructs a theoretical framework of environmental protection subsidies, environmental tax collection, and enterprise green innovation by taking the China A-share manufacturing listed enterprises as a research sample. Further, we explore the contingency impact of the market-expected performance gap and market competition on the enterprise green innovation. The empirical results show that there is a linear positive driving relationship between environmental protection subsidies and enterprise green innovation. The relationship between environmental tax collection and enterprise green innovation is a U-shaped relationship that first inhibits and then promotes. The market expectation performance gap moderates between environmental subsidy and enterprise green innovation. Market competition plays a reinforcing role in environmental subsidy, environmental tax collection, and enterprise green innovation. These research findings are conducive to providing theoretical support and reference for the government to encourage enterprises to actively carry out green innovation in practice, thereby helping to promote green development in the country.
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http://dx.doi.org/10.1007/s11356-022-22538-3DOI Listing
August 2022

The Emergence of Resistance Under Firstline INSTI Regimens.

Infect Drug Resist 2022 6;15:4269-4274. Epub 2022 Aug 6.

Guangxi Key Laboratory of AIDS Prevention and Treatment & Guangxi Universities Key Laboratory of Prevention and Control of Highly Prevalent Disease, Nanning, Guangxi, 530021, People's Republic of China.

We reported an HIV-naïve patient from a resource-limited area who was detected with multiple resistance sites associated with nucleoside reverse transcriptase inhibitors (NRTIs) and integrase strand transfer inhibitors (INSTIs) after the failure of the initial antiviral regimen dolutegravir/lamivudine (DTG/3TC) and subsequent Bictegravir/emtricitabine/tenofovir alafenamide (BIC/FTC/TAF). On May 8, 2021, a 53-year-old man was diagnosed with AIDS, Marneffei talaromycosis and fungal esophagitis, and was suspected of having tuberculosis (TB) in Guangxi, China. His baseline HIV RNA was 559,000 copies/mL and the CD4 count was 12 cells/µL, but resistance genotype testing was not performed. The patient remained immunosuppressed (CD4 count 3 cells/µL) after 12 weeks of initial antiviral treatment (ART) with DTG/3TC. After he was switched to BIC/FTC/TAF and started anti-TB treatment, the viral load (HIV RNA 163,200 copies/mL) was not effectively controlled, and there were multiple NRTIs drug-resistant mutations (D67N, K70R, M184V, T215V, K219Q) and INSTIs mutations (E138K, G140A, S147SG, Q148R). This suggested that in resource-limited areas, for HIV-naïve patients in advanced stages with active opportunistic infections, HIV RNA>500,000 copies/mL, and low CD4 count, baseline resistance testing and increased HIV RNA testing frequency should be recommended, DTG/3TC was not recommended as initiation, and opportunistic infections should be treated promptly. In addition, switching to other INSTIs was not recommended in the absence of resistance testing and ineffective use of DTG.
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http://dx.doi.org/10.2147/IDR.S375439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365015PMC
August 2022

Additive Manufacturing of Biomaterials-Design Principles and Their Implementation.

Materials (Basel) 2022 Aug 8;15(15). Epub 2022 Aug 8.

Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology (TU Delft), Mekelweg 2, 2628 CD Delft, The Netherlands.

Additive manufacturing (AM, also known as 3D printing) is an advanced manufacturing technique that has enabled progress in the design and fabrication of customised or patient-specific (meta-)biomaterials and biomedical devices (e.g., implants, prosthetics, and orthotics) with complex internal microstructures and tuneable properties. In the past few decades, several design guidelines have been proposed for creating porous lattice structures, particularly for biomedical applications. Meanwhile, the capabilities of AM to fabricate a wide range of biomaterials, including metals and their alloys, polymers, and ceramics, have been exploited, offering unprecedented benefits to medical professionals and patients alike. In this review article, we provide an overview of the design principles that have been developed and used for the AM of biomaterials as well as those dealing with three major categories of biomaterials, i.e., metals (and their alloys), polymers, and ceramics. The design strategies can be categorised as: library-based design, topology optimisation, bio-inspired design, and meta-biomaterials. Recent developments related to the biomedical applications and fabrication methods of AM aimed at enhancing the quality of final 3D-printed biomaterials and improving their physical, mechanical, and biological characteristics are also highlighted. Finally, examples of 3D-printed biomaterials with tuned properties and functionalities are presented.
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http://dx.doi.org/10.3390/ma15155457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369548PMC
August 2022

Study on the biological mechanism of urolithin a on nasopharyngeal carcinoma .

Pharm Biol 2022 Dec;60(1):1566-1577

School of Basic Medical Sciences, Youjiang Medical University for Nationalities, Baise, PR China.

Context: Urolithin A (UroA) can inhibit the growth of many human cancer cells, but it has not be reported if UroA inhibits nasopharyngeal carcinoma (NPC) cells.

Objective: To explore the inhibitory effect of UroA on NPC and potential mechanism .

Materials And Methods: RNA-sequencing-based mechanistic prediction was conducted by comparing KEGG enrichment of 40 μM UroA-treated for 24 h with untreated CNE2 cells. The untreated cells were selected as control. After NPC cells were treated with 20-60 μM UroA, proliferation, migration and invasion of were measured by colony formation, wound healing and transwell experiments. Apoptosis, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) were measured by flow cytometry, Hoechst 33342, Rhodamine 123, JC-1 staining and ROS assay methods, respectively. Gene and protein expression were measured by RT-qPCR and Western blotting assay.

Results: RNA-sequencing and KEGG enrichment revealed UroA mainly altered the ECM receptor interaction pathway. UroA inhibited cells proliferation, epithelial-mesenchymal-transition pathway, migration and invasion with IC values of 34.72 μM and 44.91 μM, induced apoptosis, MMP depolarization and increase ROS content at a concentration of 40 μM. UroA up-regulated E-cadherin, Bax/Bcl-2, c-caspase-3 and PARP proteins, while inhibiting COL4A1, MMP2, MMP9, N-cadherin, Vimentin and Snail proteins at 20-60 μM. Moreover, co-treatment of UroA (40 μM) and NAC (5 mM) could reverse the effect of UroA on apoptosis-related proteins.

Discussion And Conclusions: RNA-sequencing technology based on bioinformatic analyses may be applicable for studiying the mechanism of drugs for tumour treatment.
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http://dx.doi.org/10.1080/13880209.2022.2106251DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377270PMC
December 2022

Linking oxidative and reductive clusters to prepare crystalline porous catalysts for photocatalytic CO reduction with HO.

Nat Commun 2022 Aug 10;13(1):4681. Epub 2022 Aug 10.

School of Chemistry, South China Normal University, Guangzhou, 510006, P.R. China.

Mimicking natural photosynthesis to convert CO with HO into value-added fuels achieving overall reaction is a promising way to reduce the atmospheric CO level. Casting the catalyst of two or more catalytic sites with rapid electron transfer and interaction may be an effective strategy for coupling photocatalytic CO reduction and HO oxidation. Herein, based on the MOF ∪ COF collaboration, we have carefully designed and synthesized a crystalline hetero-metallic cluster catalyst denoted MCOF-TiCu with spatial separation and functional cooperation between oxidative and reductive clusters. It utilizes dynamic covalent bonds between clusters to promote photo-induced charge separation and transfer efficiency, to drive both the photocatalytic oxidative and reductive reactions. MCOF-TiCu exhibits fine activity in the conversion of CO with water into HCOOH (169.8 μmol gh). Remarkably, experiments and theoretical calculations reveal that photo-excited electrons are transferred from Ti to Cu, indicating that the Cu cluster is the catalytic reduction center.
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http://dx.doi.org/10.1038/s41467-022-32449-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365760PMC
August 2022

Targeting autophagy regulation in NLRP3 inflammasome-mediated lung inflammation in COVID-19.

Clin Immunol 2022 Aug 6:109093. Epub 2022 Aug 6.

Sichuan Key Medical Laboratory of New Drug Discovery and Druggability Evaluation, Materia Medica, Luzhou Key Laboratory of Activity Screening and Druggability Evaluation for Chinese Materia Medica, School of Pharmacy; Education Ministry Key Laboratory of Medical Electrophysiology, School of Preclinical Medicine, Southwest Medical University, Luzhou 646000, China; State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa 99078, Macau SAR, China. Electronic address:

Coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Emerging evidence indicates that the NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome is activated, which results in a cytokine storm at the late stage of COVID-19. Autophagy regulation is involved in the infection and replication of SARS-CoV-2 at the early stage and the inhibition of NLRP3 inflammasome-mediated lung inflammation at the late stage of COVID-19. Here, we discuss the autophagy regulation at different stages of COVID-19. Specifically, we highlighted the therapeutic potential of autophagy activators in COVID-19 by inhibiting the NLRP3 inflammasome, thereby avoiding the cytokine storm. We hope this review provides enlightenment for the use of autophagy activators targeting the inhibition of the NLRP3 inflammasome, specifically the combinational therapy of autophagy modulators with the inhibitors of the NLRP3 inflammasome, antiviral drugs, or anti-inflammatory drugs in the fight against COVID-19.
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http://dx.doi.org/10.1016/j.clim.2022.109093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356669PMC
August 2022

A novel greedy adaptive ant colony algorithm for shortest path of irrigation groups.

Math Biosci Eng 2022 06;19(9):9018-9038

College of Information Science and Technology, Shihezi University, Shihezi, 832000, China.

With the full-scale implementation of facility agriculture, the laying of a water distribution network (WDN) on farmland plays an important role in irrigating crops. Especially in large areas of farmland, with the parameters of moisture sensors, the staff can divide the WDN into several irrigation groups according to the soil moisture conditions in each area and irrigate them in turn, so that irrigation can be carried out quickly and efficiently while meeting the demand for irrigation. However, the efficiency of irrigation is directly related to the pipe length of each irrigation group of the WDN. Obtaining the shortest total length of irrigation groups is a path optimization problem. In this paper, a grouped irrigation path model is designed, and a new greedy adaptive ant colony algorithm (GAACO) is proposed to shorten the total length of irrigation groups. To verify the effectiveness of GAACO, we compare it with simple modified particle swarm optimization (SMPSO), chaos-directed genetic algorithms (CDGA) and self-adaptive ant colony optimization (SACO), which are currently applied to the path problem. The simulation results show that GAACO can effectively shorten the total path of the irrigation group for all cases from 30 to 100 water-demanding nodes and has the fastest convergence speed compared to SMPSO, CDGA and SACO. As a result, GAACO can be applied to the shortest pipeline path problem for irrigation of farmland groups.
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http://dx.doi.org/10.3934/mbe.2022419DOI Listing
June 2022

Design for Bedridden Elderly: Presenting Pressure Ulcer Product Design Based on Anthropometric Characteristics.

Comput Intell Neurosci 2022 30;2022:4895038. Epub 2022 Jul 30.

College of Art and Design, Beijing University of Technology, Beijing 100124, China.

Pressure ulcers are a type of injury that causes tissue ischemia, a deficiency of nutrition and oxygen to the tissues, and, eventually, tissue necrosis when an area of skin is placed under constant pressure for an extended length of time. With the acceleration of the aging process, the problem of providing care for pressure ulcers for the bedridden elderly becomes increasingly urgent. This study conducts a field survey based on the research status of 221 disabled elderly in 16 communities on 7 typical streets in Beijing, focusing on the problem of pressure ulcer complications caused by bedridden. An automatic inflatable airbag mattress is designed according to anthropometric dimensions of bedridden elderly, pressure ulcer-prone areas, and the decompression standard, so that the airbag mattress can reduce the pressure in its initial shape. To achieve accurate control of the pressure in the pressure-prone areas of an airbag, air pressure control system is proposed which can control airbags individually and link multiple airbags, evaluating the safety of pressure ulcer points based on the data from sensors and making corresponding air pressure changes to reduce the possibility of generating pressure ulcers. The proposed pressure ulcer preventing system will be an efficient healthcare tool for families who had elderly bedridden patients, patients with chronic degenerative disease side effects, and terminal and postsurgical patients, as well as femur fractures, in their homes.
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http://dx.doi.org/10.1155/2022/4895038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356806PMC
August 2022

Toward a unified nomenclature for strains with hyper-biofilm phenotypes.

Trends Microbiol 2022 Aug 5. Epub 2022 Aug 5.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China. Electronic address:

Hyper-biofilm strains form robust biofilms, are highly adaptable, and form highly tolerant subpopulations in biofilms grown in vivo and in vitro. Such subpopulations are formed by a wide range of bacteria and thus have been given different names in different species. This situation calls for the establishment of a unified nomenclature for strains with hyper-biofilm phenotypes.
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http://dx.doi.org/10.1016/j.tim.2022.07.007DOI Listing
August 2022

Complex genome assembly based on long-read sequencing.

Brief Bioinform 2022 Aug 6. Epub 2022 Aug 6.

College of Information and Computer Engineering, Northeast Forestry University, Harbin, 150040, China.

High-quality genome chromosome-scale sequences provide an important basis for genomics downstream analysis, especially the construction of haplotype-resolved and complete genomes, which plays a key role in genome annotation, mutation detection, evolutionary analysis, gene function research, comparative genomics and other aspects. However, genome-wide short-read sequencing is difficult to produce a complete genome in the face of a complex genome with high duplication and multiple heterozygosity. The emergence of long-read sequencing technology has greatly improved the integrity of complex genome assembly. We review a variety of computational methods for complex genome assembly and describe in detail the theories, innovations and shortcomings of collapsed, semi-collapsed and uncollapsed assemblers based on long reads. Among the three methods, uncollapsed assembly is the most correct and complete way to represent genomes. In addition, genome assembly is closely related to haplotype reconstruction, that is uncollapsed assembly realizes haplotype reconstruction, and haplotype reconstruction promotes uncollapsed assembly. We hope that gapless, telomere-to-telomere and accurate assembly of complex genomes can be truly routinely achieved using only a simple process or a single tool in the future.
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http://dx.doi.org/10.1093/bib/bbac305DOI Listing
August 2022

Human Nasal Organoids Model SARS-CoV-2 Upper Respiratory Infection and Recapitulate the Differential Infectivity of Emerging Variants.

mBio 2022 Aug 8:e0194422. Epub 2022 Aug 8.

Department of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Konggrid.194645.b, Pokfulam, Hong Kong, China.

The human upper respiratory tract, specifically the nasopharyngeal epithelium, is the entry portal and primary infection site of respiratory viruses. Productive infection of SARS-CoV-2 in the nasal epithelium constitutes the cellular basis of viral pathogenesis and transmissibility. Yet a robust and well-characterized model of the nasal epithelium remained elusive. Here we report an organoid culture system of the nasal epithelium. We derived nasal organoids from easily accessible nasal epithelial cells with a perfect establishment rate. The derived nasal organoids were consecutively passaged for over 6 months. We then established differentiation protocols to generate 3-dimensional differentiated nasal organoids and organoid monolayers of 2-dimensional format that faithfully simulate the nasal epithelium. Moreover, when differentiated under a slightly acidic pH, the nasal organoid monolayers represented the optimal correlate of the native nasal epithelium for modeling the high infectivity of SARS-CoV-2, superior to all existing organoid models. Notably, the differentiated nasal organoid monolayers accurately recapitulated higher infectivity and replicative fitness of the Omicron variant than the prior variants. SARS-CoV-2, especially the more transmissible Delta and Omicron variants, destroyed ciliated cells and disassembled tight junctions, thereby facilitating virus spread and transmission. In conclusion, we establish a robust organoid culture system of the human nasal epithelium for modeling upper respiratory infections and provide a physiologically-relevant model for assessing the infectivity of SARS-CoV-2 emerging variants. An model of the nasal epithelium is imperative for understanding cell biology and virus-host interaction in the human upper respiratory tract. Here we report an organoid culture system of the nasal epithelium. Nasal organoids were derived from readily accessible nasal epithelial cells with perfect efficiency and stably expanded for more than 6 months. The long-term expandable nasal organoids were induced maturation into differentiated nasal organoids that morphologically and functionally simulate the nasal epithelium. The differentiated nasal organoids adequately recapitulated the higher infectivity and replicative fitness of SARS-CoV-2 emerging variants than the ancestral strain and revealed viral pathogenesis such as ciliary damage and tight junction disruption. Overall, we established a human nasal organoid culture system that enables a highly efficient reconstruction and stable expansion of the human nasal epithelium in culture plates, thus providing a facile and robust tool in the toolbox of microbiologists.
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http://dx.doi.org/10.1128/mbio.01944-22DOI Listing
August 2022

Synergistic Manipulation of Hydrogen Evolution and Zinc Ion Flux in Metal-Covalent Organic Frameworks for Dendrite-free Zn-based Aqueous Batteries.

Angew Chem Int Ed Engl 2022 Aug 8. Epub 2022 Aug 8.

South China Normal University, school of chemistry, Nanjing wenyuan road No. 1, 51006, Guangzhou, CHINA.

Zn-based aqueous batteries have attracted much attention because of their high theoretical-capacity, safety, and low-cost, yet the H2-evolution, qualification or inhibition mechanism investigations that are closely related to the dendrite-growth are rare and challenging. Herein, a series of zincophilic metal-covalent organic frameworks (e.g., Zn-AAn-COF, Zn-DAAQ-COF, and Zn-DAA-COF) have been explored as model-platforms to manipulate the H2-evolution and Zn2+ flux. Best of them, Zn-AAn-COF based cell only produces 0.002 mmol h-1 cm-2 H2, which is > 2 orders of magnitude lower than bare Zn. Noteworthy, it affords high stability for 3000 cycles (overpotential, < 79.1 mV) at 20 mA cm-2 in symmetric-cell and enhanced cycling-stability up to 6000 cycles at 2000 mA g-1 in the assembled full-battery. Besides, mechanistic characterizations show that Zn-AAn-COF can enhance the energy-barrier of H2-evolution and homogenize the ion-distribution or electric-filed to achieve high performance.
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http://dx.doi.org/10.1002/anie.202210871DOI Listing
August 2022

Burden of tuberculosis and its association with socio-economic development status in 204 countries and territories, 1990-2019.

Front Med (Lausanne) 2022 22;9:905245. Epub 2022 Jul 22.

Department of Burns and Plastic and Wound Repair Surgery, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China.

Background: Tuberculosis (TB) always runs in the forefront of the global burden when it comes to infectious diseases. Tuberculosis, which can lead to impairment of quality of life, financial hardship, discrimination, marginalization, and social barriers, is a major public health problem. The assessment of TB burden and trend can provide crucial information for policy decision and planning, and help countries in the world to achieve the goal of sustainable development of ending the epidemic of TB in 2030.

Methods: All data are from the Global Burden of Disease 2019 (GBD 2019) database, which analyzed the burden trend of age-standardized incidence, DALYs, and deaths rate in TB and HIV/AIDS-infected TB over the past 30 years. Also, GBD 2019 not only analyzed the burden distribution of TB in 204 countries and main regions of the world but also analyzed the relationship between the burden of global TB and the socio-demographic Index (SDI).

Results: The age-standardized incidence, age-standardized disability-adjusted life years (DALYs), and age-standardized deaths rate for HIV-negative TB were 10,671.45 (9,395.60-12,194.10), 59,042.45 (53,684.78-64,641.53), and 1,463.62 (1,339.24-1,602.71) (95% CI, per 100,000 person-years) in 2019, respectively. Age-standardized incidence, age-standardized DALYs, and age-standardized deaths rate of HIV/AIDS-XDR-TB (95% CI, per 1,000 person-years) were 2.10 (1.51-2.90), 64.23 (28.64-117.74), and 1.01 (0.42-1.86), respectively. We found that TB is inversely proportional to SDI, the age-standardized incidence, DALYs, and deaths rate low burden countries were in high SDI areas, while high burden countries were in low SDI areas. The global TB showed a slow decline trend, but the age-standardized incidence of HIV-positive TB was increasing, and mainly distributed in sub-Saharan Africa.

Conclusion: Age-standardized incidence, age-standardized DALYs, and age-standardized deaths rate of TB is related to SDI, and the burden of low SDI countries is lighter than that of high SDI countries. Without effective measures, it will be difficult for countries around the world to achieve the goal of ending the TB epidemic by 2030. Effective control of the spread of TB requires concerted efforts from all countries in the world, especially in the countries with low SDI, which need to improve the diagnosis and preventive measures of TB and improve the control of HIV/AIDS-TB.
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http://dx.doi.org/10.3389/fmed.2022.905245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355511PMC
July 2022

Scalable and Reconfigurable Green Electronic Textiles with Personalized Comfort Management.

ACS Nano 2022 Aug 5. Epub 2022 Aug 5.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, P.R. China.

Electronic textiles, inherited with the wearability of conventional clothes, are deemed fundamental for emerging wearable electronics, particularly in the Internet of Things era. However, the electronic waste produced by electronic textiles will further exacerbate the severe pollution in traditional textiles. Here, we develop a large-scale green electronic textile using renewable bio-based polylactic acid and sustainable eutectic gallium-indium alloys. The green electronic textile is extremely abrasion resistant and can degrade naturally in the environment even if abrasion produces infinitesimal amounts of microplastics. The mass loss and performance change rates of the reconstituted green electronic textiles are all below 5.4% after going through the full-cycle recycling procedure. This green electronic textile delivers high physiological comfort (including electronic comfort and thermal-moisture comfort), enables wireless power supply (without constraints by, e.g., wires and ports), has 2 orders of magnitude better air and moisture permeability than the body requires, and can lower skin temperature by 5.2 °C.
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http://dx.doi.org/10.1021/acsnano.2c04252DOI Listing
August 2022

Coronaviruses exploit a host cysteine-aspartic protease for replication.

Nature 2022 Aug 3. Epub 2022 Aug 3.

State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.

Highly pathogenic coronaviruses including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Middle East respiratory syndrome coronavirus (MERS-CoV), and SARS-CoV-1 vary in their transmissibility and pathogenicity. However, infection by all three viruses results in substantial apoptosis in cell culture and in patient tissues, suggesting a potential link between apoptosis and pathogenesis of coronaviruses. Here we show that a cysteine-aspartic protease of the apoptosis cascade, caspase-6, serves as an important host factor for efficient coronavirus replication. We demonstrate that caspase-6 cleaves coronavirus nucleocapsid (N) proteins, generating N fragments that serve as interferon (IFN) antagonists, thus facilitating virus replication. Inhibition of caspase-6 substantially attenuates lung pathology and body weight loss of SARS-CoV-2-infected golden Syrian hamsters and improves the survival of mouse-adapted MERS-CoV (MERS-CoV)-infected human DPP4 knock-in (hDPP4 KI) mice. Overall, our study reveals how coronaviruses exploit a component of the host apoptosis cascade to facilitate virus replication.
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http://dx.doi.org/10.1038/s41586-022-05148-4DOI Listing
August 2022

The Prognostic Value of Tumor Size, Volume and Tumor Volume Reduction Rate During Concurrent Chemoradiotherapy in Patients With Cervical Cancer.

Front Oncol 2022 14;12:934110. Epub 2022 Jul 14.

Department of Radiation Oncology, Sichuan Cancer Hospital & Institute, Sichuan Cancer Prevention and Control Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Objective: This study aimed to investigate the relationship between prognostic and tumor parameters of cervical cancer patients, such as tumor size (TS), tumor volume (TV), and tumor volume reduction rate (TVRR) after external beam radiotherapy.

Methods: A total of 217 patients with advanced cervical cancer, classified as Federation of Gynecology and Obstetrics (FIGO) IIa-IVa, were enrolled in the study. Pre- and mid-RT pelvic magnetic resonance imaging (MRI) were performed twice, during RT and just before brachytherapy.

Results: The median follow-up time was 51 months (range, 7-111 months). The 5-year overall survival (OS), progression-free survival (PFS), and local failure-free survival (LFFS) rates were 81.3, 85.1, and 92.9%, respectively. Multivariate analysis revealed that tumor parameters including FIGO stage >II (Hazard Ratio, 2.377 and 95% confidence interval [CI], 1.091-5.182; = 0.029), pre-RT TV >61.6 cm (HR, 0.417 and 95% CI, 0.188-0.926; = 0.032), and mid-RT TV >11.38 cm (HR, 3.192 and 95% CI, 1.094-9.316; = 0.034) were observably associated with OS. Univariate analysis showed that the tumor volume reduction rate (TVRR) was dramatically associated with overall survival (HR, 0.204 and 95% CI 0.033-1.282; 0.001) and local failure-free survival ( = 0.050).

Conclusions: In this retrospective study, TVRR and mid-radiotherapy tumor volume are independent and strong prognostic parameters for patients with local advanced cervical cancer receiving CCRT.
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http://dx.doi.org/10.3389/fonc.2022.934110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329537PMC
July 2022

Genomic Analysis Uncovers the Prognostic and Immunogenetic Feature of Pyroptosis in Gastric Carcinoma: Indication for Immunotherapy.

Front Cell Dev Biol 2022 13;10:906759. Epub 2022 Jul 13.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

Crosstalk between pyroptosis and tumor immune microenvironment (TIME) in cancer has yet to be elucidated. Herein, we aimed to explore the role of pyroptosis and its association with TIME in gastric cancer. Unsupervised clustering was performed to identify the pyroptosis-related clusters. Pyroptosis risk score was constructed using LASSO Cox regression. Clinicopathological and genetic data of pyroptosis clusters and pyroptosis risk scores were explored. Reproducibility of pyroptosis risk score in predicting response to immunotherapy and screening potential antitumor drugs was also investigated. Three pyroptosis clusters with distinct prognosis, immune cell fractions and signatures, were constructed. A low-pyroptosis risk score was characterized by increased activated T-cell subtype and M1 macrophage, decreased M2 macrophage, higher MSI status, and TMB. Meanwhile, low-score significantly correlated with PD-L1 expression, antigen presentation markers, and IFN-γ signature. The 5-year AUCs of PRS were 0.67, 0.62, 0.65, 0.67, and 0.67 in the TCGA, three external public and one real-world validation (SYSUCC) cohorts. Multivariable analyses further validated the prognostic performance of the pyroptosis risk scoring system, with HRs of 2.43, 1.83, 1.78, 2.35, and 2.67 (all < 0.05) in the five cohorts. GSEA indicated significant enrichment of DNA damage repair pathways in the low-score group. Finally, the pyroptosis risk scoring system was demonstrated to be useful in predicting response to immunotherapy, and in screening potential antitumor drugs. Our study highlights the crucial role of interaction between pyroptosis and TIME in gastric cancer. The pyroptosis risk scoring system can be used independently to predict the survival of individuals and their response to immunotherapy.
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http://dx.doi.org/10.3389/fcell.2022.906759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328384PMC
July 2022

A Novel PARP Inhibitor YHP-836 For the Treatment of BRCA-Deficiency Cancers.

Front Pharmacol 2022 13;13:865085. Epub 2022 Jul 13.

State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

PARP inhibitors have clinically demonstrated good antitumor activity in patients with BRCA mutations. Here, we described YHP-836, a novel PARP inhibitor, YHP-836 demonstrated excellent inhibitory activity for both PARP1 and PARP2 enzymes. It also allosterically regulated PARP1 and PARP2 via DNA trapping. YHP-836 showed cytotoxicity in tumor cell lines with BRCA mutations and induced cell cycle arrest in the G2/M phase. YHP-836 also sensitized tumor cells to chemotherapy agents . Oral administration of YHP-836 elicited remarkable antitumor activity either as a single agent or in combination with chemotherapy agents . These results indicated that YHP-836 is a well-defined PARP inhibitor.
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http://dx.doi.org/10.3389/fphar.2022.865085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326368PMC
July 2022

Ectomycorrhizal and non-mycorrhizal rhizosphere fungi increase root-derived C input to soil and modify enzyme activities: A C pulse labelling of Picea abies seedlings.

Plant Cell Environ 2022 Jul 31. Epub 2022 Jul 31.

Department of Agroecology, Bayreuth Center of Ecology and Environmental Research (BayCEER), University of Bayreuth, Bayreuth, Germany.

Consequences of interactions between ectomycorrhizal fungi (EcMF) and non-mycorrhizal rhizosphere fungi (NMRF) for plant carbon (C) allocation belowground and nutrient cycling in soil remain unknown. To address this topic, we performed a mesocosm study with Norway spruce seedlings [Picea abies (L.) H. Karst] inoculated with EcMF, NMRF, or a mixture of both (MIX). CO pulse labelling of spruce was applied to trace and visualize the C incorporation into roots, rhizohyphosphere and hyphosphere. Activities and localization of enzymes involved in the C, nitrogen (N) and phosphorus (P) cycling were visualized using zymography. Spruce seedlings inoculated with EcMF and NMRF allocated more C to soils (EcMF: 10.7%; NMRF: 3.5% of total recovered C) compared to uninoculated control seedlings. The C activity in the hyphosphere was highest for EcMF and lowest for NMRF. In the presence of both, NMRF and EcMF (MIX), the C activity was 64% lower compared with EcMF inoculation alone. This suggests a suppressed C allocation via EcMF likely due to the competition between EcMF and NMRF for N and P. Furthermore, we observed 57% and 49% higher chitinase and leucine-aminopeptidase activities in the rhizohyphosphere of EcMF compared to the uninoculated control, respectively. In contrast, β-glucosidase activity (14.3 nmol cm  h ) was highest in NMRF likely because NMRF consumed rhizodeposits efficiently. This was further supported by that enzyme stoichiometry in soil with EcMF shifted to a higher investment of nutrient acquisition enzymes (e.g., chitinase, leucine-aminopeptidase, acid phosphatase) compared to NMRF inoculation, where investment in β-glucosidase increased. In conclusion, the alleviation of EcMF from C limitation promotes higher activities of enzymes involved in the N and P cycle to cover the nutrient demand of EcMF and host seedlings. In contrast, C limitation of NMRF probably led to a shift in investment towards higher activities of enzymes involved in the C cycle.
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http://dx.doi.org/10.1111/pce.14413DOI Listing
July 2022

Radiomics for Detection of the EGFR Mutation in Liver Metastatic NSCLC.

Acad Radiol 2022 Jul 27. Epub 2022 Jul 27.

Department of Scientific Research and Academic, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang, 110042, P.R. China. Electronic address:

Rationale And Objectives: The research aims to investigate whether MRI radiomics on hepatic metastasis from primary nonsmall cell lung cancer (NSCLC) can be used to differentiate patients with epidermal growth factor receptor (EGFR) mutations from those with EGFR wild-type, and develop a prediction model based on combination of primary tumor and the metastasis.

Materials And Methods: A total of 130 patients were enrolled between Aug. 2017 and Dec. 2021, all pathologically confirmed harboring hepatic metastasis from primary NSCLC. The pyradiomics was used to extract radiomics features from intra- and peritumoral areas of both primary tumor and metastasis. The least absolute shrinkage and selection operator (LASSO) regression was applied to identify most predictive features and to develop radiomics signatures (RSs) for prediction of the EGFR mutation status. The receiver operating characteristic (ROC) curve analysis was performed to assess the prediction capability of the developed RSs.

Results: A RS-Primary and a RS-Metastasis were derived from the primary tumor and metastasis, respectively. The RS-Combine by combination of the primary tumor and metastasis achieved the highest prediction performance in the training (AUCs, RS-Primary vs. RS-Metastasis vs. RS-Combine, 0.826 vs. 0.821 vs. 0.908) and testing (AUCs, RS-Primary vs. RS-Metastasis vs. RS-Combine, 0.760 vs. 0.791 vs. 0.884) set. The smoking status showed significant difference between EGFR mutant and wild-type groups (p < 0.05) in the training set.

Conclusion: The study indicates that hepatic metastasis-based radiomics can be used to detect the EGFR mutation. The developed multiorgan combined radiomics signature may be helpful to guide individual treatment strategies for patients with metastatic NSCLC.
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http://dx.doi.org/10.1016/j.acra.2022.06.016DOI Listing
July 2022

Highly stable acetylcholinesterase electrochemical biosensor based on polymerized ionic liquids microgel for pesticides detection.

Mikrochim Acta 2022 07 29;189(8):300. Epub 2022 Jul 29.

Yingkou Institute of Technology, Yingkou, 115014, China.

A highly stable electrochemical biosensor for pesticide detection was developed. For the first time polymeric ionic liquids (PILs) were introduced to construct an acetylcholinesterase (AChE) biosensor . AChE was entrapped in PILs microspheres through an emulsion polymerization reaction, where negatively charged Au nanoparticles (Au NPs) can be immobilized by the positively charged PILs, leading to improved catalytic performance. The results suggest that the positively charged PILs not only provide a biocompatible microenvironment around the enzyme molecule, stabilizing its biological activity and preventing its leakage, but also act as a modifiable interface allowing other components with electron transport properties to be loaded onto the polymer substrate, thus providing an efficient electron transport channel for the entrapped enzyme. More notably, when AChE was immobilized in a positively charged environment, the active site is closer to the electrode, promoting faster electron transfer. The detection limits of the constructed electrochemical biosensor [email protected]@Au NPs/GCE toward carbaryl and dichlorvos (DDVP) were 5.0 × 10 ng ml and 3.9 × 10 ng ml, in a wide linear range of 6.3 × 10-8.8 × 10 ng ml and 1.3 × 10-1.4 × 10 ng ml, respectively. More importantly, the biosensor has high thermal and storage stability, which facilitates rapid field analysis of fruits and vegetables in a variety of climates. In addition, the biosensor reported has good repeatability and selectivity and has high accuracy in the analysis of peaches, tap water, and other types of samples.
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http://dx.doi.org/10.1007/s00604-022-05383-6DOI Listing
July 2022

AirMeasurer: open-source software to quantify static and dynamic traits derived from multiseason aerial phenotyping to empower genetic mapping studies in rice.

New Phytol 2022 Jul 28. Epub 2022 Jul 28.

State Key Laboratory of Crop Genetics & Germplasm Enhancement, Academy for Advanced Interdisciplinary Studies, Jiangsu Collaborative Innovation Center for Modern Crop Production Co-sponsored by Province and Ministry, Nanjing Agricultural University, Nanjing, 210095, China.

Low-altitude aerial imaging, an approach that can collect large-scale plant imagery, has grown in popularity recently. Amongst many phenotyping approaches, unmanned aerial vehicles (UAVs) possess unique advantages as a consequence of their mobility, flexibility and affordability. Nevertheless, how to extract biologically relevant information effectively has remained challenging. Here, we present AirMeasurer, an open-source and expandable platform that combines automated image analysis, machine learning and original algorithms to perform trait analysis using 2D/3D aerial imagery acquired by low-cost UAVs in rice (Oryza sativa) trials. We applied the platform to study hundreds of rice landraces and recombinant inbred lines at two sites, from 2019 to 2021. A range of static and dynamic traits were quantified, including crop height, canopy coverage, vegetative indices and their growth rates. After verifying the reliability of AirMeasurer-derived traits, we identified genetic variants associated with selected growth-related traits using genome-wide association study and quantitative trait loci mapping. We found that the AirMeasurer-derived traits had led to reliable loci, some matched with published work, and others helped us to explore new candidate genes. Hence, we believe that our work demonstrates valuable advances in aerial phenotyping and automated 2D/3D trait analysis, providing high-quality phenotypic information to empower genetic mapping for crop improvement.
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http://dx.doi.org/10.1111/nph.18314DOI Listing
July 2022

Antibacterial activity and mechanism of ginger extract against Ralstonia solanacearum.

J Appl Microbiol 2022 Jul 26. Epub 2022 Jul 26.

College of Horticulture and Gardening, Yangtze University, Jingzhou, China.

Aims: The current study aimed to determine the chemical compositions of ginger extract (GE) and to assess the antibacterial activities of GE against the ginger bacterial wilt pathogen Ralstonia solanacearum and to screen their mechanisms of action.

Methods And Results: A total of 393 compounds were identified by using ultra-performance liquid chromatography and tandem-mass spectrometry. The antibacterial test indicated that GE had strong antibacterial activity against R. solanacearum and that the bactericidal effect exhibited a dose-dependent manner. The minimum inhibitory concentration and minimum bactericidal concentration of R. solanacearum were 3.91 and 125 mg/ml, respectively. The cell membrane permeability and integrity of R. solanacearum were destroyed by GE, resulting in cell content leakage, such as electrolytes, nucleic acids, proteins, extracellular adenosine triphosphate and exopoly saccharides. In addition, the activity of cellular succinate dehydrogenase and alkaline phosphatase of R. solanacearum decreased gradually with an increase in the GE concentration. Scanning electron microscopy analysis revealed that GE treatment changed the morphology of the R. solanacearum cells. Further experiments demonstrated that GE delayed or slowed the occurrence of bacterial wilt on ginger.

Conclusions: GE has a significant antibacterial effect on R. solanacearum, and the antibacterial effect is concentration dependent. The GE treatments changed the morphology, destroyed membrane permeability and integrity, reduced key enzyme activity and inhibit the synthesis of the virulence factor EPS of R. solanacearum. GE significantly controlled the bacterial wilt of ginger during infection.

Significance And Impact Of The Study: This research provides insight into the antimicrobial mechanism of GE against R. solanacearum, which will open a new application field for GE.
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http://dx.doi.org/10.1111/jam.15733DOI Listing
July 2022

A single-nucleotide polymorphism in WRKY33 promoter is associated with the cold sensitivity in cultivated tomato.

New Phytol 2022 Jul 26. Epub 2022 Jul 26.

Department of Horticulture, Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Yuhangtang Road 866, Hangzhou, 310058, China.

Natural variations in cis-regulatory regions often affect crop phenotypes by altering gene expression. However, the mechanism of how promoter mutations affect gene expression and crop stress tolerance is still poorly understood. In this study, by analyzing RNA-sequencing (RNA-Seq) data and reverse transcription quantitative real-time PCR validation in the cultivated tomato and its wild relatives, we reveal that the transcripts of WRKY33 are almost unchanged in cold-sensitive cultivated tomato Solanum lycopersicum L. 'Ailsa Craig' but are significantly induced in cold-tolerant wild tomato relatives Solanum habrochaites LA1777 and Solanum pennellii LA0716 under cold stress. Overexpression of SlWRKY33 or ShWRKY33 positively regulates cold tolerance in tomato. Variant of the critical W-box in SlWRKY33 promoter results in the loss of self-transcription function of SlWRKY33 under cold stress. Analysis integrating RNA-Seq and chromatin immunoprecipitation sequencing data reveals that SlWRKY33 directly targets and induces multiple kinases, transcription factors, and molecular chaperone genes, such as CDPK11, MYBS3, and BAG6, thus enhancing cold tolerance. In addition, heat- and Botrytis-induced WRKY33s expression in both wild and cultivated tomatoes are independent of the critical W-box variation. Our findings suggest nucleotide polymorphism in cis-regulatory regions is crucial for different cold sensitivity between cultivated and wild tomato plants.
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http://dx.doi.org/10.1111/nph.18403DOI Listing
July 2022

Application of a novel fluorogenic polyurethane analogue probe in polyester-degrading microorganisms screening by microfluidic droplet.

Microb Biotechnol 2022 Jul 26. Epub 2022 Jul 26.

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.

Application of polyester-degrading microorganisms or enzymes should be considered as an eco-friendly alternative to chemical recycling due to the huge plastic waste disposal nowadays. However, current impranil DLN-based screening of polyester-degrading microorganisms is time-consuming, labour-intensive and unable to distinguish polyesterases from other protease- or amidase-like enzymes. Herein, we present an approach that combined a novel synthetic fluorescent polyurethane analogue probe (FPAP), along with the droplet-based microfluidics to screen polyurethane-degrading microorganisms through fluorescence-activated droplet sorting (FADS) pipeline. The fluorescent probe FPAP exhibited a fluorescence enhancement effect once hydrolysed by polyesterases, along with a strong specificity in discriminating polyesterases from other non-active enzymes. Application of FPAP in a microfluidic droplet system demonstrated that this probe exhibited high sensitivity and efficiency in selecting positive droplets containing leaf-branch compost cutinase (LCC) enzymes. This novel fluorogenic probe, FPAP, combined with the droplet microfluidic system has the potential to be used in the exploitation of novel PUR-biocatalysts for biotechnological and environmental applications.
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http://dx.doi.org/10.1111/1751-7915.14121DOI Listing
July 2022

Genomic Investigation of Isolates Recovered From Pig Farms in Zhejiang Province, China.

Front Microbiol 2022 7;13:952982. Epub 2022 Jul 7.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, MOA Laboratory of Quality & Safety Risk Assessment for Agro-Products (Hangzhou), Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

is a common opportunistic zoonotic pathogen, and its ongoing acquisition of antimicrobial resistance genes poses challenges to clinical treatments. Human-sourced whole genomic sequencing of human isolates has been reported, but pig-sourced isolates have not been thoroughly investigated even though these animals can serve as reservoirs for human infections. In the current study, we report a molecular epidemiological investigation to unravel the antimicrobial and virulence gene risk factors for contamination in 9 pig farms in 3 different cities in Zhejiang Province, China. We collected 541 swab samples from healthy pigs and 30 were confirmed as . All 30 isolates were resistant to tetracyclines, macrolides, sulfonamides, β-lactams and chloramphenicol, and all were multiple drug-resistant and 27 were strong biofilm formers. Phylogenetic analyses indicated these 30 isolates clustered together in 2 major groups. Whole genome sequencing demonstrated that the isolates possessed 91 different antimicrobial resistance genes belonging to 30 antimicrobial classes including and All isolates contained mobile genetic elements including integrative conjugative elements (ICEs) and integrative and mobilizable elements (IMEs). Minimum inhibitory concentration (MIC) testing indicated direct correlates between cognate genes and antimicrobial resistance. We also identified 95 virulence factors, almost all isolates contained 20 fimbrial and flagellar operons, and this represents the greatest number of these operon types found in a single species among all sequenced bacterial genomes. These genes regulate biofilm formation and represent a confounding variable for treating infections. Our isolates were present in healthy animals, and multiple drug resistance in these isolates may serve as a reservoir for other intestinal and environmental Enterobacteriaceae members. This prompts us to more strictly regulate veterinary antibiotic use.
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http://dx.doi.org/10.3389/fmicb.2022.952982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300985PMC
July 2022

Targeting ACLY efficiently inhibits SARS-CoV-2 replication.

Int J Biol Sci 2022 11;18(12):4714-4730. Epub 2022 Jul 11.

State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, and Carol Yu Centre for Infection, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, People's Republic of China.

The Coronavirus Disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the biggest public health challenge the world has witnessed in the past decades. SARS-CoV-2 undergoes constant mutations and new variants of concerns (VOCs) with altered transmissibility, virulence, and/or susceptibility to vaccines and therapeutics continue to emerge. Detailed analysis of host factors involved in virus replication may help to identify novel treatment targets. In this study, we dissected the metabolome derived from COVID-19 patients to identify key host factors that are required for efficient SARS-CoV-2 replication. Through a series of metabolomic analyses, , and investigations, we identified ATP citrate lyase (ACLY) as a novel host factor required for efficient replication of SARS-CoV-2 wild-type and variants, including Omicron. ACLY should be further explored as a novel intervention target for COVID-19.
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http://dx.doi.org/10.7150/ijbs.72709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305265PMC
July 2022

Editorial: Molecular Perspectives for Plant Autophagy Regulation.

Front Plant Sci 2022 6;13:967916. Epub 2022 Jul 6.

Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, China.

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http://dx.doi.org/10.3389/fpls.2022.967916DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296813PMC
July 2022
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