Publications by authors named "Jie Zhang"

8,506 Publications

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Joint effect of indoor size-fractioned particulate matters and black carbon on cardiopulmonary function and relevant metabolic mechanism: A panel study among school children.

Environ Pollut 2022 May 23:119533. Epub 2022 May 23.

Department of Occupational and Environmental Health Sciences, School of Public Health, Peking University, Beijing, 100191, China. Electronic address:

Indoor particulate matter (PM) and black carbon (BC) are associated with adverse cardiopulmonary effect. However, the cumulative and interactive effects of the mixture of size-fractioned PMs and BC on cardiopulmonary function are not well understood, and its underlying biological mechanisms remain unclear. This repeated-measure study was conducted to assess the joint cardiopulmonary effect and metabolic mechanisms of multiple-size particles and BC among 46 children. PM, PM, PM, PM, PM and BC were monitored for 5 weekdays. Cardiorespiratory function measurements and urine samples collection were conducted three times. Untargeted-metabolomics and meet-in-metabolite approach were applied to mechanism investigation. Bayesian machine kernel regression was adopted to analyze associations among PMs, cardiopulmonary function and metabolites. Lung function and heart rate variability significantly decreased with the increased PMs and BC co-exposure (p < 0.05). The effective particles were BC, PM and PM in turn. No interaction effects of different particles on cardiopulmonary function were observed at different lag days. BC-related glucose and fatty acid increase, and PM-related branched-chain amino acid degradation were primarily observed. Other metabolisms were successively disturbed. The greatest joint effects of PMs and BC on metabolism were mainly at lag0 and lag01 day. They occurred earlier than the strongest effects on cardiopulmonary function, which were at lag01 and lag02 day. BC, PM and PM were mainly associated with cardiorespiratory indices by disturbing amino acids, glucose, lipid, isoflavone and purine metabolism. Mitochondrial productivity and antioxidation reduction are pivotal to the relevant metabolic alterations. More attention should be paid to BC and smaller-size PMs to control indoor PM pollution and its adverse effect on children.
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http://dx.doi.org/10.1016/j.envpol.2022.119533DOI Listing
May 2022

Modeling polycyclic aromatic hydrocarbons in India: Seasonal variations, sources and associated health risks.

Environ Res 2022 May 23:113466. Epub 2022 May 23.

Department of Environmental Science and Engineering, Fudan University, Shanghai, 200348, China; Department of Civil and Environmental Engineering, Louisiana State University, Baton Rouge, LA, 70803, United States. Electronic address:

Atmospheric polycyclic aromatic hydrocarbons (PAHs) are in high levels in developing countries like India. However, limited measurements are inadequate for better understanding of their ambient levels and health effects. This study predicted PAHs concentrations in atmosphere and estimated their sources and health risks in India in four representative months of winter, pre-monsoon, monsoon and post-monsoon in 2015 using an updated version of the Community Multiscale Air Quality model (CMAQ). Predicted PAHs were in agreement with observations from literature. Surface 16-PAHs were highest in winter, with a peak value of 2.5 μg/m and population-weighted average of 0.5 μg/m in northern and eastern India, where biomass burning and coal combustion were chief contributors. Pre-monsoon and monsoon had lower concentrations ∼0.2 μg/m. The incremental lifetime cancer risk (ILCR) was greater than 4E-4 in many industrial and urban areas. Exposure to PAHs resulted in 7431 excess lifetime cancer cases. Coal combustion and biomass burning were major contributors to ILCR, followed by gas and oil activities. Much higher health risks were observed in urban than in rural areas. India showed much higher levels of total PAHs and cPAHs than the U.S but moderately less than China.
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http://dx.doi.org/10.1016/j.envres.2022.113466DOI Listing
May 2022

Acetylation licenses Th1 cell polarization to constrain Listeria monocytogenes infection.

Cell Death Differ 2022 May 25. Epub 2022 May 25.

Institue of Clinical Medicine, Zhejiang Provincial People's Hospital of Hangzhou Medical College, 158 Shangtang Road, Hangzhou, Zhejiang, 310000, China.

T helper 1 (Th1) immunity is typically viewed as a critical adaptation by vertebrates against intracellular pathogens. Identifying novel targets to enhance Th1 cell differentiation and function is increasingly important for anti-infection immunity. Here, through small-molecule screening focusing on epigenetic modifiers during the in vitro Th1 cell differentiation process, we identified that the selective histone deacetylase 6 (HDAC6) inhibitors ricolinostat and nexturastat A (Nex A) promoted Th1 cell differentiation. HDAC6-depleted mice exhibit elevation of Th1 cell differentiation, and decreased severity of Listeria monocytogenes infection. Mechanistically, HDAC6 directly deacetylated CBP-catalyzed acetylation of signal transducer and activator of transcription 4 (STAT4)-lysine (K) 667 via its enzymatic activity. Acetylation of STAT4-K667 is required for JAK2-mediated phosphorylation and activation of STAT4. Stat4 mutant mice lost the ability to normally differentiate into Th1 cells and developed severe Listeria infection. Our study identifies acetylation of STAT4-K667 as an essential signaling event for Th1 cell differentiation and defense against intracellular pathogen infections, and highlights the therapeutic potential of HDAC6 inhibitors for controlling intracellular pathogen infections.
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http://dx.doi.org/10.1038/s41418-022-01017-9DOI Listing
May 2022

In-situ chemical attenuation of pharmaceutically active compounds using CaO: Influencing factors, mechanistic modeling, and cooperative inactivation of water-borne microbial pathogens.

Environ Res 2022 May 22:113531. Epub 2022 May 22.

Key Laboratory of Organic Compound Pollution Control Engineering (MOE), School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China. Electronic address:

Water polluted by pharmaceutically active compounds (PhACs) and water-borne pathogens urgently need to develop eco-friendly and advanced water treatment techniques. This paper evaluates the potential of using calcium peroxide (CaO), a safe and biocompatible oxidant both PhACs (thiamphenicol, florfenicol, carbamazepine, phenobarbital, and primidone) and pathogens (Escherichia coli, Staphylococcus aureus) in water. This paper evaluates the potential of using calcium peroxide (CaO) as a safe and biocompatible oxidant to remove both PhACs (thiamphenicol, florfenicol, carbamazepine, phenobarbital, and primidone) and pathogens (Escherichia coli, Staphylococcus aureus) in water. The increased CaO dosage increased efficiencies of PhACs attenuation and pathogens inactivation, and both exhibited pseudo-first-order degradation kinetics (R > 0.90). PhACs attenuation were mainly via oxidization (HO, •OH/O, and O) and alkaline hydrolysis (OH) from CaO. Moreover, concentrations of these reactive species and their contributions to PhACs attenuation were quantified, and mechanistic model was established and validated. Besides, possible transformation pathways of target PhACs except primidone were proposed. As for pathogen indicators, the suitable inactivation dosage of CaO was 0.1 g L. The oxidability (18-64%) and alkalinity (82-36%) generated from CaO played vital roles in pathogen inactivation. In addition, CaO at 0.01-0.1 g L can be applied in remediation of SW contaminated by PhACs and pathogenic bacteria, which can degrade target PhACs with efficiencies of 21-100% under 0.01 g L CaO, and inactivate 100% of test bacteria under 0.1 g L CaO. In short, capability of CaO to remove target PhACs and microbial pathogens reveals its potential to be used as a representative technology for the advanced treatment of waters contaminated by organic compounds and microbial pathogens.
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http://dx.doi.org/10.1016/j.envres.2022.113531DOI Listing
May 2022

Characterization of a novel type of glycogen-degrading amylopullulanase from Lactobacillus crispatus.

Appl Microbiol Biotechnol 2022 May 25. Epub 2022 May 25.

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

Glycogen is one of the major carbohydrates utilized by the human vaginal microbiota, which is commonly dominated by Lactobacillus, especially L. crispatus. An in silico analysis predicted that a type I pullulanase was involved in glycogen degradation in L. crispatus. The biochemical and genetic properties of the pullulanase still need to be determined. Here, we de novo identified the glycogen (Glg)-utilization enzyme (named GlgU) from L. crispatus through a biochemical assay. GlgU was optimally active at acidic pH, approximately 4.0 ~ 4.5, and was able to hydrolyze glycogen into low-molecular-weight malto-oligosaccharides. Actually, GlgU was a type II pullulanase (amylopullulanase) with just one catalytic domain that possessed substrate specificity toward both α-1,4 and α-1,6-glucosidic bonds. Phylogenetically, GlgU was obviously divergent from the known amylases and pullulanases (including amylopullulanases) in lactobacilli. In addition, we confirmed the catalytic activity of glgU in a nonglycogen-utilizing lactobacilli strain, demonstrating the essential role of glgU in glycogen metabolism. Overall, this study characterized a novel type of amylopullulanases, contributing to the knowledge of the glycogen utilization mechanism of the dominant species of human vaginal microbiota. KEY POINTS: • GlgU was a type II pullulanase, not a type I pullulanase predicted before. • GlgU was able to completely hydrolyze glycogen into malto-oligosaccharides. • GlgU played a key role in the metabolism of extracellular glycogen.
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http://dx.doi.org/10.1007/s00253-022-11975-2DOI Listing
May 2022

Triplex immunostrip assay for rapid diagnosis of tobacco mosaic virus, tobacco vein banding mosaic virus and potato virus Y.

Plant Dis 2022 May 25. Epub 2022 May 25.

Shandong Agricultural University, Plant Protection, NO.61, Daizong Street, Tai'an City, Shandong Province, Tai'an, Shandong, China, 271018;

Mixed virus infection has increasingly become a problem in the production of crops in recent years; therefore, a fast and accurate detection method is needed. In this study, a novel triplex immunostrip assay was developed for the simultaneous detection of tobacco mosaic virus (TMV), tobacco vein banding mosaic virus (TVBMV), and potato virus Y (PVY). The limits of detection (LODs) of this novel immunostrip reached 200 ppb (ng/mL), 1 ppm (µg/mL), and 2 ppm for TMV, PVY, and TVBMV particles, respectively. Importantly, no cross-reactivity was observed among TMV, TVBMV and PVY or to a non-target virus. When the assay was applied to suspected virus-infected tobacco, tomato and potato samples collected from fields in Southwest China, samples of single or mixed virus infection were successfully identified. In conclusion, the triplex immunostrip assay provides a fast and easy to use on-site detection method for field epidemiological studies of TMV, TVBMV, and PVY, and for managing diseases that are caused by them.
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http://dx.doi.org/10.1094/PDIS-08-21-1756-REDOI Listing
May 2022

Generation of iodinated trihalomethanes during chloramination in the presence of solid copper corrosion products.

Water Res 2022 May 17;220:118630. Epub 2022 May 17.

CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei 230026, China. Electronic address:

Copper water pipelines are widely used in water distribution systems, but the effects of solid copper corrosion products (CCPs) including CuO, CuO and Cu(OH)CO on the generation of iodinated trihalomethanes (I-THMs) during chloramination remain unknown. This study found that the formation of I-THMs during chloramination of humic acid (HA) was inhibited by the presence of CuO and CuO, but promoted with the addition of Cu(OH)CO. The negative effect of CuO and CuO is mainly exerted by promoting the decay of both NHCl and HOI. Although Cu(OH)CO also accelerated the decomposition of NHCl and HOI, it was found that the complexes formed between Cu(OH)CO and HA facilitated, through carboxyl functional groups, the reaction between HA and HOI, leading to an enhancement of I-THM generation during chloramination, which was further confirmed by model compound experiments. Additionally, this study demonstrated that the effects of solid CCPs on I-THM generation during chloramination were solid CCP- and HA-concentration dependent, but almost unaffected by different initial I and Br concentrations. This study provides new insights into the health risks caused by the corrosion of copper water pipelines, especially in areas intruded by sea water.
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http://dx.doi.org/10.1016/j.watres.2022.118630DOI Listing
May 2022

Experimental observations of marginal criticality in granular materials.

Proc Natl Acad Sci U S A 2022 May 24;119(22):e2204879119. Epub 2022 May 24.

School of Physics and Astronomy, Shanghai Jiao Tong University, 200240 Shanghai, China.

SignificanceAmorphous materials, such as grains, foams, colloids, and glasses, are ubiquitous in nature and our daily life. They can undergo glass transitions or jamming transitions to obtain rigidity either by fast quench or compression, but show subtle changes in the structures compared to the liquid states or liquid-like states. Recent progress on the first-principle replica theory unifies the glass transition and the jamming transition and points out the marginal phase with fractal free-energy landscape within the stable glass phase. Independently, marginal stability analysis predicts the relations between the exponents of the marginal phase. Here, we perform experiments with photoelastic disks and provide direct evidence of these theories in real-world amorphous materials.
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http://dx.doi.org/10.1073/pnas.2204879119DOI Listing
May 2022

Modulation of Neuronal Activity and Saccades at Theta Rhythm During Visual Search in Non-human Primates.

Neurosci Bull 2022 May 24. Epub 2022 May 24.

The Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen, 518055, China.

Active exploratory behaviors have often been associated with theta oscillations in rodents, while theta oscillations during active exploration in non-human primates are still not well understood. We recorded neural activities in the frontal eye field (FEF) and V4 simultaneously when monkeys performed a free-gaze visual search task. Saccades were strongly phase-locked to theta oscillations of V4 and FEF local field potentials, and the phase-locking was dependent on saccade direction. The spiking probability of V4 and FEF units was significantly modulated by the theta phase in addition to the time-locked modulation associated with the evoked response. V4 and FEF units showed significantly stronger responses following saccades initiated at their preferred phases. Granger causality and ridge regression analysis showed modulatory effects of theta oscillations on saccade timing. Together, our study suggests phase-locking of saccades to the theta modulation of neural activity in visual and oculomotor cortical areas, in addition to the theta phase locking caused by saccade-triggered responses.
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http://dx.doi.org/10.1007/s12264-022-00884-zDOI Listing
May 2022

Gene and protein expression of dorsal root ganglion sensory receptors in normotensive and hypertensive male rats.

Am J Physiol Regul Integr Comp Physiol 2022 May 24. Epub 2022 May 24.

Geriatric Research, Education, and Clinical Center, Salt Lake City VAMC, Salt Lake City, UT, United States.

The exercise pressor reflex (EPR), a neurocirculatory control mechanism, is exaggerated in hypertensive humans and rats. Disease-related abnormalities within the afferent arm of the reflex loop, including mechano- and metabosensitive receptors located at the terminal end of group III/IV muscle afferents, may contribute to the dysfunctional EPR in hypertension. Using control (WKY) and spontaneous hypertensive (SHR) rats, we examined dorsal root ganglion (DRG) gene and protein expression of molecular receptors recognized as significant determinants of the EPR. Twelve lumbar DRGs (6-left, 6-right) were harvested from each of 10 WKY (arterial blood pressure (MAP): 96±9mmHg) and 10 SHR (MAP: 144±9mmHg). DRGs from the left side were used for protein expression (Western blotting; normalized to GAPDH), whereas right-side DRGs (i.e., parallel structure) were used to determine mRNA levels (RNA-sequencing, normalized to TPM). Analyses focused on metabosensitive (ASIC3, Bradykinin-receptor B2, EP4, P2X3, TRPv1) and mechanosensitive (Piezo1/2) receptors. While Piezo1 was similar in both groups (P=0.75), protein expression for all other receptors was significantly higher in SHR compared to WKY. With the exception of a greater Bradykinin-receptor B2 in SHR (P<0.05), mRNA expression of all other receptors was not different between groups (P>0.18). The higher protein content of these sensory receptors in SHR indirectly supports the previously proposed hypothesis that the exaggerated EPR in hypertension is, in part, due to disease-related abnormalities within the afferent arm of the reflex loop. The upregulated receptor content, combined with normal mRNA levels, insinuates that posttranscriptional regulation of sensory receptor protein expression might be impaired in hypertension.
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http://dx.doi.org/10.1152/ajpregu.00007.2022DOI Listing
May 2022

Preparation and identification of an antioxidant peptide from wheat embryo albumin and characterization of its Maillard reaction products.

J Food Sci 2022 May 23. Epub 2022 May 23.

Henan Provincial Key Laboratory of Biological Processing and Nutritional Function of Wheat, School of Biological Engineering, Henan University of Technology, Zhengzhou, PR China.

Wheat embryo albumin (WEA) extracted from wheat embryo possesses multiple effects including antioxidant, anti-inflammatory, and immunoregulatory effects. In this study, a single factor experiment was conducted to determine the optimal enzymolysis conditions of WEA. Five components (F1-F5) were obtained via ultrafiltration, among which F3 (molecular weight 3-5 kDa) displayed the best antioxidant activity. WEA and F3 were characterized by transmission electron microscopy, scanning electron microscopy, circular dichroism spectrum analysis, and amino acid composition tests. Results revealed that F3 significantly increased the contents of β-tablets, aromatic amino acids, and hydrophobic amino acids compared to WEA. LC-MS/MS analysis demonstrated that F3 had more tyrosine and histidine moieties than WEA. Moreover, analysis of the Maillard reaction products (MRPs) showed that F3-MRPs had strong browning strength, ultraviolet absorption, higher number of free amino acids, and umami amino acid ratio compared with WEA. In conclusion, enzymolysis can improve the functional properties of WEA, which broadens the application spectrum of WEA in food and pharmaceutical fields. PRACTICAL APPLICATION: This study provides a new approach for identifying potential antioxidants and developing functional foods from WEA, and broadens the application spectrum of wheat germ resources.
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http://dx.doi.org/10.1111/1750-3841.16191DOI Listing
May 2022

A Potential Prognostic Marker PRDM1 in Pancreatic Adenocarcinoma.

J Oncol 2022 13;2022:1934381. Epub 2022 May 13.

Medical School, Ningbo University, Ningbo, Zhejiang, China.

Pancreatic adenocarcinoma (PAAD) is a major threat to people's health. PRDM1 is a transcription factor with multiple functions, and its functions have been validated in a variety of tumors; however, there are few studies reported on PRDM1 in PAAD. Using the GEPIA2 database, this research found that PRDM1 expression in PAAD was significantly higher than that in normal pancreatic tissue. The Kaplan-Meier Plotter database showed that high expression of PRDM1 in PAAD has a poor prognosis, suggesting that PRDM1 may be a potential prognostic marker in PAAD. The cBioPortal database shows that the expression of PRDM1 in PAAD is significantly correlated with its methylation degree. Further analysis on the coexpressed genes of PRDM1 in PAAD was performed by using LinkedOmics database to explore potential mechanisms. Based on gene enrichment analysis, PRDM1 was implicated in many pathways involved in tumor progression. In the construction of a PPI network of PRDM1 and its coexpressed gene protein via the STRING database, we found that PRDM1 may be involved in the pathogenesis and development of PAAD. TIMER database suggested that a high level of PRDM1 has a significant positive correlation with macrophages, neutrophils, and DCs. Potential methylation sites of PRDM1 were found through DNMIVD database, and MethSurv database explored eight sites which were significantly related with the prognosis of PAAD. In conclusion, PRDM1 may work as a prognostic marker or even provide a potential therapeutic strategy in PAAD.
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http://dx.doi.org/10.1155/2022/1934381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123419PMC
May 2022

The Efficacy of Percutaneous Transhepatic Gallbladder Drainage Combined with Gallbladder-Preserving Cholecystolithotomy in High-Risk Patients with Acute Calculous Cholecystitis.

J Inflamm Res 2022 10;15:2901-2910. Epub 2022 May 10.

General Surgery Department, Beijing Rehabilitation Hospital of Capital Medical University, Beijing, 100144, People's Republic of China.

Objective: This study aimed to investigate the value of combining percutaneous transhepatic gallbladder drainage (PTGD) with gallbladder-preserving cholecystolithotomy (GPC) in high-risk patients with acute calculous cholecystitis.

Methods: Clinical data from 74 high-risk patients with acute calculous cholecystitis, admitted to our hospital between October 2018 and September 2021, were analyzed retrospectively. All the patients underwent PTGD, and 59 of them underwent delayed cholecystectomy, while 14 patients were subjected to GPC 8-12 weeks after the PTGD; one patient, whose life expectancy was fewer than 6 months, was not treated for gallstones after PTGD.

Results: In all 74 patients, symptom remission was achieved after the PTGD therapy, and the incidence of catheter-related complications was 10.8%. Among the 59 patients who underwent delayed cholecystectomy (DC) after PTGD, there was a complication incidence of 6.8%. Of the 14 patients who underwent GPC after the PTGD, 13 patients were subjected to the removal of drainage tubes, 1 patient received cholecystostomy catheter draining externally, and two patients (14.3%) had complications. There were no perioperative deaths.

Conclusion: Percutaneous transhepatic gallbladder drainage, combined with GPC, is a safe and effective treatment that is suitable for high-risk patients with acute calculous cholecystitis who cannot receive DC. This combined method allows for early acute cholecystitis to settle, helps to remove gallstones at a later stage, and solves the problem of long-term tube drainage after PTGD.
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http://dx.doi.org/10.2147/JIR.S363610DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114648PMC
May 2022

Mitigating Bias and Error in Machine Learning to Protect Sports Data.

Authors:
Jie Zhang Jia Li

Comput Intell Neurosci 2022 11;2022:4777010. Epub 2022 May 11.

Zhengzhou Preschool Education College, Zhengzhou, Henan 450000, China.

One of the essential processes in modern sports is doping control. In recent years, specialized methods of artificial intelligence and large-scale data analysis have been used to make faster and simpler detection of violations of international regulations on the use of banned substances. The smart systems in question depend directly on the quality of the data used, as high-quality data will produce algorithmic approaches of correspondingly high quality and accuracy. It is evident that there are many sources of errors in data collections and intentional algorithmic interventions that may result from cyber-attacks, so end-users of artificial intelligence technologies should be able to know the exact origins of data and analytical methods of these data at an algorithmic level. Given that artificial intelligence systems based on incomplete or discriminatory data can lead to inaccurate results that violate the fundamental rights of athletes, this paper presents an advanced model for mitigating bias and error in machine learning to protect sports data, using convolutional neural network (ConvNet) with high-precise class activation maps (HiPrCAM). It is an innovative neural network interpretability technique, wherewith the addition of Bellman reinforcement learning (BRL) and Broyden-Fletcher-Goldfarb-Shanno (BFGS) optimization; it can produce high-precision maps that deliver high definition, clarity, and the input and output capture when the algorithm makes a prediction. The evaluation of the proposed system uses the Shapley value solution from the cooperative game theory to provide algorithmic performance propositions for each of the produced results, assigning partial responsibility to parts of the architecture based on the impact that the efforts have on the relative success measurement, which it has been preset.
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http://dx.doi.org/10.1155/2022/4777010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117075PMC
May 2022

Silibinin Schiff Base Derivatives Counteract CCl-Induced Acute Liver Injury by Enhancing Anti-Inflammatory and Antiapoptotic Bioactivities.

Drug Des Devel Ther 2022 16;16:1441-1456. Epub 2022 May 16.

Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China.

Background: Silibinin (Sil), a flavonoid lignan-like natural compound derived from milk thistle seeds, has been used to treat hepatic diseases, including early-phase hepatocirrhosis and fatty liver, for many years. However, its poor water solubility limits its gastrointestinal absorption and bioavailability. It clinical use has been limited due to its slow onset of action. Faced with this problem, research on the derivatives of silibinin has been receiving much attention.

Purpose: A series of silibinin derivatives with good biosafety and higher hepatoprotective activity were obtained by a safe, efficient and green chemical synthesis method.

Patients And Methods: First, the carbonyl group in the structure of silibinin was used to obtain silibinin Schiff base derivatives by dehydration condensation with the carboxyl group in the sulfur-containing amino acid. Next, relevant experiments were performed to characterize the structure, physical form and solubility of the derivatives. Then, toxicity tests of the derivatives were performed in LO-2 cells and SD rats to evaluate their biosafety. Finally, the anti-inflammatory and antiapoptotic activities were observed using a carbon tetrachloride (CCl)-induced acute liver injury model in C57BL/6J mice using silibinin as a control.

Results: The studies showed that SS and ST behaved as amorphous substances and showed a significant increase in solubility compared to silibinin. These two derivatives showed low toxicity in biosafety tests and higher bioactivity (anti-inflammatory and anti-apoptotic) than silibinin against acute liver injury induced by CCl.

Conclusion: Two silibinin derivatives (SS and ST) obtained by the Schiff base reaction improved the solubility of the silibinin parent nucleus in biological media with the help of the hydrophilic and amorphous morphology of the ligand. The low toxicity in vivo and in vitro ensures the biosafety of the derivatives. The hepatoprotective activity (anti-inflammatory and anti-apoptotic) was significantly improved compared to silibinin.
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http://dx.doi.org/10.2147/DDDT.S356847DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122151PMC
May 2022

Corrigendum: Sacral Curvature in Addition to Sacral Ratio to Assess Sacral Development and the Association With the Type of Anorectal Malformations.

Front Pediatr 2022 6;10:922448. Epub 2022 May 6.

Department of Pediatric Surgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.

[This corrects the article DOI: 10.3389/fped.2021.732524.].
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http://dx.doi.org/10.3389/fped.2022.922448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121193PMC
May 2022

Transcriptome and Metabolome Analysis of the Synthesis Pathways of Allelochemicals in .

ACS Omega 2022 May 4;7(19):16803-16816. Epub 2022 May 4.

College of Life Sciences, North China University of Science and Technology, Tangshan 063210, China.

(Crofton weed) is an invasive weed in more than 30 countries. It inhibits the growth of surrounding plants by releasing allelochemicals during its invasion. However, the synthetic pathways and molecular mechanisms of its allelochemicals have been rarely reported. In this study, the related genes and pathways of allelochemicals in were analyzed. Transcriptome analysis showed that differentially expressed genes (DEGs) were mainly enriched in the phenylpropanoid biosynthetic pathway and flavonoid biosynthetic pathway. Thirty-three DEGs involved in the synthesis of allelochemicals were identified, and 30 DEGs showed significant differences in blades and stems. Six allelochemicals were identified from blades and stems by ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Correlation analysis of genes and metabolites showed a strong correlation between the five genes and allelochemicals. In addition, this study supplemented the biosynthetic pathway of (HHO). It was found that acyclic sesquiterpene synthase (NES), δ-cadinene synthase (TPS), and cytochrome P450 (P450) were involved in the synthesis of HHO. These findings provide a dynamic spectrum consisting of allelochemical metabolism and a coexpression network of allelochemical synthesis genes in .
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http://dx.doi.org/10.1021/acsomega.2c01816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118424PMC
May 2022

Structural, Physicochemical, and Functional Properties of Wheat Bran Insoluble Dietary Fiber Modified With Probiotic Fermentation.

Front Nutr 2022 4;9:803440. Epub 2022 May 4.

School of Biological Engineering, Henan University of Technology, Zhengzhou, China.

Insoluble dietary fiber (IDF) were isolated from wheat bran (WB) after microbial fermentation with single or mixed strain [ or mixed lactic acid bacteria ( and with ration of 1:1)]. Structure, physicochemical, functional properties, and antioxidant activity of the wheat bran insoluble dietary fiber (W-IDF) modified by fermentation were studied. Fourier transformed infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM) analysis suggested the successful modification of W-IDF. After fermentation with and mixed lactic acid bacteria, the water retention capacity (WRC), oil retention capacity (ORC), and water swelling capacity (WSC) of W-IDF were improved. The sodium cholate adsorption capacity (SCAC), and cation exchange capacity (CEC) of W-IDF modified with fermentation were significantly increased. Although the cholesterol adsorption capacity (CAC) of W-IDF decreased after modification with probiotic fermentation, nitrite ion adsorption capacity (NIAC), and total phenolic content (TPC) were enhanced. Additionally, W-IDF modified by fermentation with or mixed lactic acid bacteria exhibited superior antioxidant capacity verified by DPPH, ABTS and total reducing power assays. Results manifested that microbial fermentation is a promising methods to modify the W-IDF to provide high-quality functional IDF for food processing and human health management.
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http://dx.doi.org/10.3389/fnut.2022.803440DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9116382PMC
May 2022

Protective Effect of Nalbuphine Combined With Dexmedetomidine on the Lungs of Children With Atelectasis and Foreign Body in the Bronchus During the Perioperative Period.

Front Physiol 2022 5;13:799183. Epub 2022 May 5.

Department of Anesthesiology, Zhengzhou University First Affiliated Hospital, Zhengzhou, China.

The present study aims to explore the protective effect of nalbuphine combined with dexmedetomidine on the lungs of children with atelectasis who have a foreign body in a bronchus during the perioperative period. A total of 180 patients whose computed tomography scan showed atelectasis and a foreign body in a bronchus were randomly divided into three groups: group C (conventional anesthesia group), group D (dexmedetomidine group), and group N + D (nalbuphine combined with the dexmedetomidine group). The following indicators were recorded: 1) heart rate (HR) and mean arterial pressure (MAP) prior to induction (T), at bronchoscope placement (T), at intubation after surgery (T), at tube removal (T), 10 min after tube removal (T), 20 min after tube removal (T), and at awaking (T); 2) monocyte toll-like receptors (TLRs) TLR⁃2, TLR⁃4, tumor necrosis factor α, interleukin 6, oxygenation index, and the B-line sum at T, T, 2 h (T), and 24 h (T) after tube removal; and 3) hospital stay after surgery. Compared with group C, in group D and group N + D, 1) the HR and MAP at T∼T were lower; 2) the inflammatory factor indicator and B-line sum were lower, and the oxygenation index was higher at T and T; 3) the agitation and cough scores were decreased during tube removal; and 4) the Ramsay sedation score was higher, and ventilator weaning time was shortened at T∼T ( < 0.05). Compared with group D, in group N + D, 1) the inflammatory factor indicator and B-line sum were lower at T; 2) the oxygenation index was higher ( < 0.05). Compared with groups C and D, in group N + D, the length of hospital stay was decreased ( < 0.05). In patients with atelectasis and a foreign body in a bronchus during the perioperative period, nalbuphine combined with dexmedetomidine may be capable of reducing the oxidative stress response, improving the oxygenation index, decreasing the pulmonary fluid content, protecting the lung, and facilitating postoperative recovery.
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http://dx.doi.org/10.3389/fphys.2022.799183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117643PMC
May 2022

Recent advances of chemosensors for nerve agents.

Chem Asian J 2022 May 22. Epub 2022 May 22.

Xi'an Jiaotong University, School of life science and technology, 28, Xianning West Road, Xi'an, Shaanxi, 710049, XiAn, CHINA.

Nerve agents are a branch of organophosphorus (OP) compounds that chemically cause irreversible inhibition of acetylcholinesterase, thereby failing the hydrolysis of acetylcholine, resulting in severe neurological disease and death in organisms. Thus, the unreasonable use of such compounds threatens public and national security, so it is a high demand to establish portable, low-cost, sensitive, and highly selective methods for their precise detection. In this review, we introduced the recent development of spectroscopy-based optical chemosensors that aimed for the detection of nerve agent, focusing on the progress made in the molecular design, sensing principle and applications. We also conclude this Review by highlighting the current challenges associated with the future development and outlook.
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http://dx.doi.org/10.1002/asia.202200284DOI Listing
May 2022

Smart Polycationic Hydrogel Dressing for Dynamic Wound Healing.

Small 2022 May 22:e2201620. Epub 2022 May 22.

Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, P. R. China.

It is challenging for traditional wound dressings to adapt to the complex and changeable environment, due to the lack of stable, efficient, and continuous bactericidal activity. They also cannot be satisfied in a multifunctional sensing platform to reconstruct skin sensory functions for human health monitoring. A multifunctional hydrogel dressing is developed here for the treatment of infected wounds and human health monitoring, which is based on alginate and polycation. The in situ polymerization and solvent displacement method are used to functionalize the hydrogel for the improvement of antifreezing, water retention, and environmental adaptability, as well as the adhesion and photothermal property. As a wound dressing, the as-prepared hydrogel exhibits an excellent antibacterial property against both Escherichia coli and Staphylococcus aureus. In a rat model of full-thickness wound infection, it significantly accelerates the healing of infected wounds with a high healing rate of 96.49%. In the further multifunctional sensory tests, the hydrogel shows multiple response modes of strain, pressure and temperature, and sensing stability. An idea is provided here to develop a smart hydrogel dressing that can accelerate wound healing and achieve human health monitoring.
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http://dx.doi.org/10.1002/smll.202201620DOI Listing
May 2022

Environmental metal exposure, seminal plasma metabolome and semen quality: Evidence from Chinese reproductive-aged men.

Sci Total Environ 2022 May 17;838(Pt 2):155860. Epub 2022 May 17.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China. Electronic address:

Environmental metal exposure has been associated with decreasing semen quality, but the effects of multiple metal exposure on seminal plasma metabolome remain obscure. In this study, semen and repeated urine samples from 551 volunteers were collected in Wuhan City. Heavy metals and trace elements were measured using inductively coupled plasma mass spectrometer, and seminal plasma metabolomes were acquired using liquid chromatography coupled with high-resolution mass spectrometry. Weighted gene co-expression network analysis showed more than half of the seminal plasma metals were associated with specific metabolite modules, whereas only a few urine metals presented weak associations, indicating that seminal plasma may be an ideal biological sample for male reproductive biomarker discovery and exposure risk assessment. Seminal plasma zinc (Zn) and selenium (Se) concentrations were significantly associated with 22 metabolites (e.g., glycerophospholipids, acyl-carnitines and amino acid derivatives). Among these metabolites, acyl-carnitines were positively associated with semen quality and sperm concentration. Moreover, acyl-carnitines were associated with both Zn and Se exposure, indicating the potential role of carnitine pathway in their toxicity mechanism. Our findings suggest that seminal plasma metabolome connects Zn and Se exposure and sperm concentrations in Chinese men of reproductive age.
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http://dx.doi.org/10.1016/j.scitotenv.2022.155860DOI Listing
May 2022

Transgenerational effects of parental bisphenol S exposure on zebrafish (Danio rerio) reproduction.

Food Chem Toxicol 2022 May 18;165:113142. Epub 2022 May 18.

College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China. Electronic address:

Bisphenol S (BPS) is extensively used for production of polycarbonates and other commodities, and is often detected in environment and biota. Parental BPS exposure has been reported to interfere with reproductive development of offspring, but limited information is available on its multigenerational reproductive toxicity. In our present study, zebrafish (Danio rerio) were exposed to BPS (1 and 100 μg/L) from 3 hpf to 120 dpf, and the effects on reproduction, sex steroid hormones, DNA methylation levels and gene transcription involved in steroidogenesis and DNA methylation were investigated in unexposed F1-2 offspring. The results showed that 100 μg/L BPS exposure increased DNA methylation in F1 testes, and 1 μg/L BPS led to DNA methylation in F2 ovaries. The increased DNA methylation levels led to decreased expression of steroidogenic enzymes, including cyp11a, cyp17 and 3βhsd, which might be a main reason for the elevated plasma 17β-estradiol and decreased testosterone levels. In addition, sex ratio indicated a female dominance trend, and reproductive capacity of male fish was severely impaired. Overall, these findings suggest that parental BPS exposure impairs reproductive development of unexposed offspring via DNA methylation and BPS-induced epigenetic modification inheritance has a long-term effect on the fitness and sustainability of fish populations.
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http://dx.doi.org/10.1016/j.fct.2022.113142DOI Listing
May 2022

Remote Programming in Patients With Parkinson's Disease After Deep Brain Stimulation: Safe, Effective, and Economical.

Front Neurol 2022 3;13:879250. Epub 2022 May 3.

Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, China.

Objective: The purpose of this study was to evaluate the safety, efficiency, and cost expenditure of remote programming in patients with Parkinson's disease (PD) after deep brain stimulation (DBS).

Methods: A total of 74 patients who underwent DBS at the Department of Neurosurgery, Zhongnan Hospital of Wuhan University between June 2018 and June 2020 were enrolled in this study. There were 27 patients in the remote programming group and 47 patients in the outpatient programming group. Clinical data, programming efficiency, adverse events, expenditure, and satisfaction were compared between the two groups.

Results: A total of 36 times of remote programming were performed on the 27 patients in the remote programming group, and four had mild adverse events during programming, and the adverse events disappeared within 1 week. The satisfaction questionnaire showed that 97.3% of the patients were satisfied with the surgical effect. The patients in the remote programming group (88.9%) were more likely to receive long-term programming after DBS than the patients in the outpatient programming group (74.5%). The Parkinsonism symptoms improved in both programming groups. The majority (18/27) of patients in the remote programming group lived away from the programming center, while the majority (27/47) of patients in the outpatient programming group lived in Wuhan, where the programming center was located ( = 0.046). The cost per patient per programming was US$ 43.5 in the remote programming group and $59.5 (56-82.7) in the outpatient programming group ( < 0.001). The median time cost for each visit was 30 min (25-30) in the remote programming group and 150 min (135-270.0) in the outpatient programming group ( < 0.001).

Conclusion: Remote programming is safe and effective after DBS in patients with Parkinson's disease. Moreover, it reduces expenditure and time costs for patients and achieves high satisfaction, particularly for patients living far from programming centers.
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http://dx.doi.org/10.3389/fneur.2022.879250DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111520PMC
May 2022

Inversion of Nitrogen Concentration in Apple Canopy Based on UAV Hyperspectral Images.

Sensors (Basel) 2022 May 4;22(9). Epub 2022 May 4.

National Apple Engineering and Technology Research Center, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.

As the major nutrient affecting crop growth, accurate assessing of nitrogen (N) is crucial to precise agricultural management. Although improvements based on ground and satellite data nitrogen in monitoring crops have been made, the application of these technologies is limited by expensive costs, covering small spatial scales and low spatiotemporal resolution. This study strived to explore an effective approach for inversing and mapping the distributions of the canopy nitrogen concentration (CNC) based on Unmanned Aerial Vehicle (UAV) hyperspectral image data in a typical apple orchard area of China. A Cubert UHD185 imaging spectrometer mounted on a UAV was used to obtain the hyperspectral images of the apple canopy. The range of the apple canopy was determined by the threshold method to eliminate the effect of the background spectrum from bare soil and shadow. We analyzed and screened out the spectral parameters sensitive to CNC, including vegetation indices (VIs), random two-band spectral indices, and red-edge parameters. The partial least squares regression (PLSR) and backpropagation neural network (BPNN) were constructed to inverse CNC based on a single spectral parameter or a combination of multiple spectral parameters. The results show that when the thresholds of normalized difference vegetation index (NDVI) and normalized difference canopy shadow index (NDCSI) were set to 0.65 and 0.45, respectively, the canopy's CNC range could be effectively identified and extracted, which was more refined than random forest classifier (RFC); the correlation between random two-band spectral indices and nitrogen concentration was stronger than that of other spectral parameters; and the BPNN model based on the combination of random two-band spectral indices and red-edge parameters was the optimal model for accurately retrieving CNC. Its modeling determination coefficient (R) and root mean square error (RMSE) were 0.77 and 0.16, respectively; and the validation R and residual predictive deviation (RPD) were 0.75 and 1.92. The findings of this study can provide a theoretical basis and technical support for the large-scale, rapid, and non-destructive monitoring of apple nutritional status.
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http://dx.doi.org/10.3390/s22093503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100912PMC
May 2022

A Multi-Mode Switching Variational Bayesian Adaptive Kalman Filter Algorithm for the SINS/PNS/GMNS Navigation System of Pelagic Ships.

Sensors (Basel) 2022 Apr 28;22(9). Epub 2022 Apr 28.

Hangzhou Innovation Institute, Beihang University, Hangzhou 310051, China.

The ocean-going environment is complex and changeable with great uncertainty, which poses a huge challenge to the navigation ability of ships working in the pelagic ocean. In this paper, in an attempt to deal with the complex uncertain interference that the environment may bring to the strap-down inertial navigation system/polarization navigation system/geomagnetic navigation system (SINS/PNS/GMNS) integrated navigation system, the multi-mode switching variational Bayesian adaptive Kalman filter (MMS-VBAKF) algorithm is proposed. To be more specific, to identify the degrees of measurement interference more effectively, we design an interference evaluation and multi-mode switching mechanism using the original polarization information and geomagnetic information. Through this mechanism, the interference to the SINS/PNS/GMNS navigation system is divided into three cases. In case of slight interference (case SI), the variational Bayesian method is adopted directly to solve the problem that the statistical characteristics of measurement noise are unknown. By the fixed-point iteration mechanism, the statistical properties of the measurement noise and the system states can be estimated adaptively in real time. In case of interference-tolerance (case TI), the estimation of the statistical characteristics of measurement noise need to weigh the measurement information at the moment and a priori value information comprehensively. In case of excessive interference (case EI), the SINS/PNS/GMNS integrated navigation system will perform mode switching and filtering system reconstruction in advance. Then, the information fusion and navigation states estimation can be completed. Consequently, the reliability, robustness, and accuracy of the SINS/PNS/GMNS integrated navigation system can be guaranteed. Finally, the effectiveness of the algorithm is illustrated by the simulation experiments.
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http://dx.doi.org/10.3390/s22093372DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100650PMC
April 2022

A Digital Twin-Based Operation Status Monitoring System for Port Cranes.

Sensors (Basel) 2022 Apr 22;22(9). Epub 2022 Apr 22.

National NC System Engineering Research Center, Huazhong University of Science and Technology, Wuhan 430074, China.

To address the problems of the lack of an online data simulation test environment, the poor openness of data collection, and the low degree of data visualization in the online control process of port cranes, an operation state monitoring system framework for port cranes based on digital twins is proposed. In this framework, the digital twin port crane is used as the core, and the multi-sensor data acquisition method, OPC UA information model, and plug-in programming method are combined to realize multi-source heterogeneous virtual and real data fusion. The digital twin crane monitoring system based on this framework can fulfil the following functions: crane historical operation process reproduction, control program simulation testing, synchronous mapping simulation, and remote control. In order to verify the proposed method, a digital twin-based physical platform for monitoring a rail-mounted gantry crane (RMGC) was built, in which a virtual test of anti-swing control and a digital twin monitoring experiment were carried out. The results show that the virtual RMGC can test the control algorithm and map the movement process of the physical RMGC, and the crane operation monitoring system has high real-time performance and good visualization effect. In addition, the remote control of the software platform is accurate and effective.
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http://dx.doi.org/10.3390/s22093216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103463PMC
April 2022

An Inclusive Entrepreneurial Path Model Based on Rural Digital Entrepreneurship Data in Zhejiang Province Using Few-Shot Learning.

Comput Intell Neurosci 2022 9;2022:8015681. Epub 2022 May 9.

Zhejiang Wanli University, 8 Qianhu South Road, Ningbo, Zhejiang 315100, China.

The main strategic direction of promoting rural revitalization is to achieve high-quality rural development, maintain the dominant position of farmers, and promote common prosperity of farmers. In recent years, the sustainable development of economy has raised farmers' awareness of starting their own businesses, and their entrepreneurial enthusiasm has been heating up. Based on the empirical survey data of typical rural areas in the Zhejiang Province and structural equation model analysis technology, this paper makes an empirical analysis of the relationship among network embeddedness, entrepreneurial resources, and entrepreneurial ability. The results show that knowledge resources have a significant negative impact on farmers' inclusive entrepreneurial behavior. The dual embedment of social network and industrial network has a significant positive impact on migrant workers' access to knowledge resources and operational resources needed for entrepreneurship, and the entrepreneurial ability of migrant workers mainly depends on operational resources. Under the background of common prosperity, we should continue to implement the policy of supporting farmers' inclusive entrepreneurship and encouraging industrial and commercial capital to enter rural areas, and at the same time, provide rural areas with agricultural knowledge, agricultural skills, and market guidance to promote employment.
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http://dx.doi.org/10.1155/2022/8015681DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110124PMC
May 2022

VGN: Value Decomposition With Graph Attention Networks for Multiagent Reinforcement Learning.

IEEE Trans Neural Netw Learn Syst 2022 May 18;PP. Epub 2022 May 18.

Although value decomposition networks and the follow on value-based studies factorizes the joint reward function to individual reward functions for a kind of cooperative multiagent reinforcement problem, in which each agent has its local observation and shares a joint reward signal, most of the previous efforts, however, ignored the graphical information between agents. In this article, a new value decomposition with graph attention network (VGN) method is developed to solve the value functions by introducing the dynamical relationships between agents. It is pointed out that the decomposition factor of an agent in our approach can be influenced by the reward signals of all the related agents and two graphical neural network-based algorithms (VGN-Linear and VGN-Nonlinear) are designed to solve the value functions of each agent. It can be proved theoretically that the present methods satisfy the factorizable condition in the centralized training process. The performance of the present methods is evaluated on the StarCraft Multiagent Challenge (SMAC) benchmark. Experiment results show that our method outperforms the state-of-the-art value-based multiagent reinforcement algorithms, especially when the tasks are with very hard level and challenging for existing methods.
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http://dx.doi.org/10.1109/TNNLS.2022.3172572DOI Listing
May 2022
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