Publications by authors named "Jie Zeng"

484 Publications

Telocytes and their structural relationships with surrounding cell types in the skin of silky fowl by immunohistochemistrical, transmission electron microscopical and morphometric analysis.

Poult Sci 2021 Jun 29;100(9):101367. Epub 2021 Jun 29.

College of Life Science and Engineering, Foshan University, Foshan 528231, China; College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China. Electronic address:

Telocytes (TCs), a novel type of interstitial cells, were identified in various animals. Since TCs have not observed in avian skin, hence, we carried out immunohistochemistrical and transmission electron microscopical studies in the skin of the silky fowl to investigate the TCs. TCs appear as CD34, c-Kit, and PDGFRα immunopositive. The elongated TCs with 2 long and thin telopodes (Tps) are located in the dermis. Generally, a TC possesses a fusiform, ovoid and polygonal cell body with 2 Tps (lengths = 5.27-21.85 μm), which are uneven in thickness including thick sections - podoms (diameters = 0.40-0.47 μm) and thin sections - podomers (diameters = 0.03-0.04 μm). TCs/Tps are observed frequently in close proximity to neighboring cell types/structures, such as adipocytes, collagen fibers, and capillaries. Under a magnified field, homocellular TCs/Tps contacts are observed through gap junctions (distances = 0.01-0.05 μm), whereas some of TCs/Tps have heterocellular close contacts by point contacts with surrounding cells, including stem cells and melanocytes. The multivisicular bodies, especially exosomes (diameters = 0.09-0.23 μm) releasing from TCs/Tps are observed in close proximity to TCs/Tps. Our results illustrated that the novel type of interstitial cells - TCs are present in the dermis of the silky fowl, and they have special structural relationships with surrounding cell types. The study provides histological evidence for TCs involvement in intercellular communication, skin regeneration, and pigmentogenesis in avian skin.
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http://dx.doi.org/10.1016/j.psj.2021.101367DOI Listing
June 2021

Different response pattern of cyanobacteria at development and maintenance stage to potassium permanganate oxidation.

Authors:
Xi Li Jie Zeng Xin Yu

J Hazard Mater 2021 Jun 25;419:126492. Epub 2021 Jun 25.

College of The Environment & Ecology, Xiamen University, Xiamen 361102, China. Electronic address:

Occurrence of successive cyanobacterial blooms in source waters can continuously impair drinking water quality. Previous studies have separately investigated potassium permanganate (KMnO) to treat high-viability cyanobacteria at just one stage of either development or maintenance. However, maintenance stage exhibited significantly higher cell-density and extracellular organic matters (EOMs) than development stage, which may result in a different KMnO oxidation pattern. In this study, kinetics of oxidant decay, membrane integrity loss, and toxin degradation of high-viability cyanobacteria at both stages were compared. Results showed that cyanobacteria at maintenance stage became more resistant to KMnO oxidation than that at development stage, since elevated cell-density and more proteins involved in EOMs resulted in lower oxidant exposure at this stage. Meanwhile, elevated cyanobacterial biomass became the main competitors to decrease toxin degradation efficiency at maintenance stage, leading to incapacity to degrade extracellular toxin to below safety guideline of 1 μg L. Consequently, comparing with the best strategy for development stage (6 mg min L, no membrane damage), a higher oxidant exposure (12 mg min L) was recommended to treat cyanobacteria at maintenance stage even with slight membrane damage (19%), since it degraded extracellular toxin to below safety guideline and achieved the highest removal ratio of EOMs. Overall, this study demonstrated that stage of cyanobacteria can strongly affect KMnO oxidation pattern, and it is necessary for water supplies to optimize KMnO treatments depending on bloom stage.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126492DOI Listing
June 2021

Photocatalytic Conversion of Methane: Recent Advancements and Prospects.

Angew Chem Int Ed Engl 2021 Jul 26. Epub 2021 Jul 26.

Hefei National Laboratory for Physical Sciences at the Microscale, Hefei National Laboratory for Physical Sciences at the Microscale, 96 Jinzhai Road, 230026, Hefei, CHINA.

Abundant and affordable methane is not only high-quality fossil energy, but also the raw material for the synthesis of value-added chemicals. Solar-energy-driven conversion of methane offers a promising approach to directly transform methane to valuable energy sources under mild conditions, but remains as a grand challenge currently. In this Review, recent advancements of photocatalytic conversion of methane are systematically summarized. Insights into the construction of effective semiconductor-based photocatalysts from the perspective of light-absorption units and active centers are proposed and discussed in detail. Moreover, photocatalytic performances of methane conversion over various catalysts are also presented and classified by oxidant systems. Lastly, challenges and future perspectives concerning mechanistic study and practical application of photocatalytic methane oxidation are introduced.
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http://dx.doi.org/10.1002/anie.202108069DOI Listing
July 2021

Doping regulation in transition metal compounds for electrocatalysis.

Chem Soc Rev 2021 Jul 26. Epub 2021 Jul 26.

Hefei National Laboratory for Physical Sciences at the Microscale, Key Laboratory of Strongly-Coupled Quantum Matter Physics of Chinese Academy of Sciences, Key Laboratory of Surface and Interface Chemistry and Energy Catalysis of Anhui Higher Education Institutes, Department of Chemical Physics, University of Science and Technology of China, Hefei, Anhui 230026, P. R. China.

In electrocatalysis, doping regulation has been considered as an effective method to modulate the active sites of catalysts, providing a powerful means for creating a large variety of highly efficient catalysts for various reactions. Of particular interest, there has been growing research concerning the doping of two-dimensional transition-metal compounds (TMCs) to optimize their electrocatalytic performance. Despite the previous achievements, mechanistic insights of doping regulation in TMCs for electrocatalysis are still lacking. Herein, we provide a systematic overview of doping regulation in TMCs in terms of background, preparation, impacts on physicochemical properties, and typical applications including the hydrogen evolution reaction, oxygen evolution reaction, oxygen reduction reaction, CO2 reduction reaction, and N2 reduction reaction. Notably, we bridge the understanding between the doping regulation of catalysts and their catalytic activities via focusing on the physicochemical properties of catalysts from the aspects of vacancy concentrations, phase transformation, surface wettability, electrical conductivity, electronic band structure, local charge distribution, tunable adsorption strength, and multiple adsorption configurations. We also discuss the existing challenges and future perspectives in this promising field.
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http://dx.doi.org/10.1039/d1cs00330eDOI Listing
July 2021

Advancing collaborations in health research and clinical trials in Sub-Saharan Africa: development and implementation of a biostatistical collaboration module in the Masters in Biostatistics Program at Stellenbosch University.

Trials 2021 Jul 22;22(1):478. Epub 2021 Jul 22.

Division of Epidemiology and Biostatistics, Department of Global Health, Stellenbosch University, Cape Town, South Africa.

Background: Sub-Saharan Africa continues to carry a high burden of communicable diseases such as TB and HIV and non-communicable diseases such as hypertension and other cardiovascular conditions. Although investment in research has led to advances in improvements in outcomes, a lot still remains to be done to build research capacity in health. Like many other regions in the world, Sub-Saharan Africa suffers from a critical shortage of biostatisticians and clinical trial methodologists.

Methods: Funded through a Fogarty Global Health Training Program grant, the Faculty of Medicine and Health Sciences at Stellenbosch University in South Africa established a new Masters Program in Biostatistics which was launched in January 2017. In this paper, we describe the development of a biostatistical and clinical trials collaboration Module, adapted from a similar course offered in the Health Research Methodology program at McMaster University.

Discussion: Guided by three core principles (experiential learning; multi-/inter-disciplinary approach; and formal mentorship), the Module aims to advance biostatistical collaboration skills of the trainees by facilitating learning in how to systematically apply fundamental statistical and trial methodological knowledge in practice while strengthening some soft skills which are necessary for effective collaborations with other healthcare researchers to solve health problems. We also share some preliminary findings from the first four cohorts that took the Module in January-November 2018 to 2021. We expect that this Module can provide an example of how to improve biostatistical and clinical trial collaborations and accelerate research capacity building in low-resource settings.

Funding Source: Fogarty International Center of the National Institutes of Health.
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http://dx.doi.org/10.1186/s13063-021-05427-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295633PMC
July 2021

Smooth muscle actin immunostain is useful for the diagnosis of Trichomonas vaginalis in vaginal smears.

Diagn Cytopathol 2021 Jul 22. Epub 2021 Jul 22.

Department of Medical Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Objective: The parasite Trichomonas vaginalis (T. vaginalis) causes one of the most common non-viral sexually transmitted infections in humans. T. vaginalis is notorious for its inconspicuous appearance in vaginal smears. It can be missed under the microscope.

Method: In the present study, we investigate the immunoreactivity of T. vaginalis to smooth muscle actin (SMA) in the vaginal smear.

Result: T. vaginalis trophozoite and pseduocyst are immunoreactive for SMA in all of the study group cases (n = 21) and in none of the control group cases (n = 21). Thus, SMA immunostain is a sensitive method for the demonstration of T. vaginalis. Moreover, the protozoan attains a conspicuous and unique appearance. By SMA immunohistochemical stain, the apperance of T. vaginalis floated freely or located in the cytoplasm of the epithelial cells is easily identified.

Conclusion: We recommend performing SMA immunostain in every vaginal smear with clinical or pathologic suspicion of trichomoniasis.
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http://dx.doi.org/10.1002/dc.24822DOI Listing
July 2021

Potassium permanganate as a promising pre-oxidant to treat low-viability cyanobacteria and associated removal of cyanotoxins and extracellular organic matters.

Authors:
Xi Li Jie Zeng Xin Yu

Water Res 2021 Jun 10;202:117353. Epub 2021 Jun 10.

College of The Environment & Ecology, Xiamen University, Xiamen, 361102, China. Electronic address:

Cell-viability of cyanobacteria declines from development to decay stage during a successive bloom. Potassium permanganate (KMnO) has demonstrated to be a superior pre-oxidant to treat high-viability cyanobacteria compared to other common oxidants (e.g., chlorine), but whether it is feasible to treat low-viability cyanobacteria is unknown. Here, effects of KMnO on membrane integrity, cyanotoxin fate and extracellular organic matters (EOMs) removal of high- and low-viability cyanobacteria were compared. Results showed that cell-viability of cyanobacteria could affect oxidant decay (k), membrane damage (k), and cyanotoxins release (k) and degradation (k) during KMnO oxidation, similar to chlorination. However, unlike chlorination, initial low dosages of KMnO (0.5 and 1 mg L) minimized membrane damage for low-viability cyanobacteria (< 27%), and continuously decrease extracellular cyanotoxins, extracellular organic matters (EOMs), and aromatic compounds to some degrees (P<0.05). High dosages of KMnO (> 2 mg L) caused severe membrane destruction (> 89%) for low-viability cyanobacteria, leading to a fast increase of extracellular cyanotoxins within 1 h. However, total/extracellular cyanotoxins were oxidized to below the safety guideline of 1 μg L after being dosed with sufficient oxidant exposure. EOMs and aromatic compounds were also reduced by 5-18% (P<0.05). Additionally, KMnO-assisted coagulation significantly improved the removal of low-viability cyanobacteria (2-5 fold). Consequently, KMnO could be a promising pre-oxidant to treat low-viability cyanobacteria at decay stage of a successive bloom.
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http://dx.doi.org/10.1016/j.watres.2021.117353DOI Listing
June 2021

Efficacy and biomarker exploration of camrelizumab combined with apatinib in the treatment of advanced primary liver cancer: a retrospective study.

Anticancer Drugs 2021 Jul 4. Epub 2021 Jul 4.

Department of Medical Oncology Department of Hepatobiliary Surgery, the First Affiliated Hospital of Guangxi Medical University, Guangxi, China.

This study was to explore the efficacy and safety of camrelizumab combined with apatinib in patients with advanced liver cancer. Moreover, the relationship between peripheral blood parameters and tumor response rate was also investigated. Patients with unresectable or recurrent primary liver cancer (PLC) who received treatment from July 2019 to July 2020 in the First Affiliated Hospital of Guangxi Medical University were included in this single-center retrospective study. The patients were treated with camrelizumab (200 mg, intravenous q2w) plus apatinib (250 mg, oral qd) until the occurrence of disease progression or unbearable toxicity. All the patients underwent blood routine test and detection of lactate dehydrogenase and serum albumin levels before treatment. The primary endpoints were objective response rate (ORR) and disease control rate (DCR). This study included a total of 45 patients. The overall ORR was 33.3% [95% confidence interval (CI),19.0-47.7] and the overall DCR was 57.8% (95% CI, 42.8-72.8). The ORR and DCR were higher in the first-line treatment than those in the second-line treatment (ORR: 45.5% vs. 21.7%, DCR: 63.6% vs. 52.3%). Median progression-free survival in the second-line treatment was 10.5 months (95% CI, 7.9-13.1, P = 0.022). Adverse events occurred in 39 (86.7%) patients. Grade 3/4 adverse reactions occurred in 7 (15.6%) patients. One patient (4.3%) was terminated from treatment due to adverse events. One patient (4.3%) died, which was potentially associated with adverse events. Subgroup analysis indicated that the remission rate in patients with high lymphocyte to monocyte ratio (H-LMR) was higher than that in patients with low lymphocyte to monocyte ratio (L-LMR) (56.25% vs. 25.93%, P = 0.047), and the remission rate in patients with high Prognostic Nutritional Index (H-PNI) was higher than that in patients with low Prognostic Nutritional Index (L-PNI) (66.7% vs. 26.5%, P = 0.046). Camrelizumab combined with apatinib in the treatment of PLC showed encouraging clinical efficacy, with tolerable toxicities. Levels of PNI and LMR may serve as predictors of the prognosis of advanced PLC patients who receive immunotherapy combined with targeted therapy.
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http://dx.doi.org/10.1097/CAD.0000000000001127DOI Listing
July 2021

Complete Degradation and Detoxification of Ciprofloxacin by a Micro-/Nanostructured Biogenic Mn Oxide Composite from a Highly Active Mn-Oxidizing Strain.

Nanomaterials (Basel) 2021 Jun 24;11(7). Epub 2021 Jun 24.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.

Ciprofloxacin (CIP), as a representative broad-spectrum antibiotic, poses a major threat to human health and the ecological environment as a result of its abuse and emissions. In this study, a highly active Mn-oxidizing bacterium, sp. CCTCC M2014168, was induced to form micro-/nanostructured biogenic Mn oxide (BMO) aggregates through continuous culturing with 1 mmoL Mn. Following the characterization of Mn oxides and the micro-/nanostructures by scanning electron microscopy, high-resolution transmission electron microscopy and X-ray diffraction assays, the BMO composites were subjected to CIP degradation and detoxification in laboratory trials. High-performance liquid chromatograph (HPLC) analysis identified that the BMO composites were capable of completely degrading CIP, and HPLC with a mass spectrometer (LC/MS) assays identified three intermediates in the degradation pathway. The reaction temperature, pH and initial ciprofloxacin concentration substantially affected the degradation efficiency of CIP to a certain extent, and the metal ions Mg, Cu, Ni and Co exerted significant inhibitory effects on CIP degradation. A toxicity test of the degradation products showed that CIP was completely detoxified by degradation. Moreover, the prepared BMO composite exhibited a high capacity for repeated degradation and good performance in continuous degradation cycles, as well as a high capacity to degrade CIP in real natural water.
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http://dx.doi.org/10.3390/nano11071660DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304510PMC
June 2021

Iron isotope of suspended particulate matter in Zhujiang River, Southwest China: Characteristics, sources, and environmental implications.

Authors:
Guilin Han Jie Zeng

Sci Total Environ 2021 Jun 22;793:148562. Epub 2021 Jun 22.

Institute of Earth Sciences, China University of Geosciences (Beijing), Beijing 100083, China.

Understanding the environmental iron cycle influenced by natural and anthropogenic processes is significant to obtain the key information on earth-surface evolution. Iron isotope compositions and elemental compositions of the suspended particulate matter (SPM) in Zhujiang River were investigated to provide key insights for the earth-surface iron cycle. The δFe values of SPM display the range from -0.05‰ to 0.34‰ (averaged 0.19‰) while the iron contents range from 0.73 wt% to 7.63 wt% (averaged 4.15 wt%). The Chemical Index of Alteration (CIA) shows that the main weathering type of SPM is intermediate weathering (mean CIA value: 79.12). While the main chemical weathering types are similar, the δFe values vary dramatically, indicating that chemical weathering is not the governing factor of δFe values of SPM. Furthermore, evidence from the enrichment factors (EF, 0.78 to 1.29) of iron and Zinc isotopes reveals that the iron input from anthropogenic activities is quite limited. The significant correlation between (Fe/Ca)SPM and (Na/Ca)SPM, (Mg/Ca)SPM, (Al/Ca)SPM and (K/Ca)SPM (0.73 < r < 0.99, p < 0.01, n = 22) and the A-CN-K diagram could confirm that clay minerals (especially smectite and illite) are important components of SPM. Meanwhile, the higher enrichment rates of La, Pr and Nd with the increasing iron contents denote heavy minerals (mainly Fe oxides/oxy-hydroxides) are also important compositions of SPM. Based on the isotopic mass balance, it shows that the iron flux of SPM to the oceans during the wet season would reduce the δFe values of the bulk ocean by 0.3%. The present study reports the iron isotope and elemental compositions of Zhujiang SPM and identifies its influencing factors (weathering, anthropogenic inputs, and minerals effect), and also provides a quantitative reference for the intriguing question on the distinct δFe values of oceans, which is beneficial for understanding iron cycle in earth-surface system.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148562DOI Listing
June 2021

Dietary intervention with sialylated lactulose affects the immunomodulatory activities of mice.

J Dairy Sci 2021 Jun 24. Epub 2021 Jun 24.

School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China.

Four sialylated lactuloses [N-acetylneuraminic acid-α2,3-lactulose (Neu5Acα2,3lactulose), N-acetylneuraminic acid-α2,6-lactulose (Neu5Acα2,6lactulose), deaminoneuraminc acid-α2,3-lactulose (Kdnα2,3lactulose), and deaminoneuraminc acid-α-2,6-lactulose (Kdnα2,6lactulose)] were reported to modulate the immunity of mice. The influences of cytokine expression, cell immunity, humoral immunity, and nonspecific immunity were investigated in our study using several techniques. Analysis via ELISA showed that cytokine expression was induced by sialylated lactulose treatment consistently in the serum and spleen. Among the 4 tested sialylated lactuloses, Neu5Acα2,6lactulose performed the best, simultaneously and appropriately promoting the expression of proinflammatory and anti-inflammatory factors in the serum and spleen. Kdnα2,3lactulose showed the best antioxidant activity according to detection of the activity of superoxide dismutase, myeloperoxidase, peroxidase, and alkaline phosphatase. Flow cytometry revealed that only Kdnα2,3lactulose significantly boosted the CD3 T lymphocyte ratio similarly to that of lactulose. Analysis of the hemolysin content to characterize humoral immunity revealed that Kdnα2,3lactulose notably increased hemolysin content compared with that in the control group. To evaluate the nonspecific immune effects of the 4 sialylated lactuloses, a fluorescence microsphere phagocytosis assay was used to analyze the phagocytosis of macrophages. Kdnα2,3lactulose still performed the best in enhancing the phagocytosis of macrophages, showing markedly increased phagocytic percentage and phagocytic index values compared with those in the control and lactulose groups. Comparing the differences of these 4 sialylated lactuloses in affecting immunity in mice revealed that Kdnα2,3lactulose had the best overall performance in influencing cytokine expression, cell immunity, humoral immunity, and nonspecific immunity. This study provides critical support for use of sialylated lactuloses as potential immunomodulators in foods.
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http://dx.doi.org/10.3168/jds.2021-20327DOI Listing
June 2021

Preliminary Study on the Safety and Efficacy of One-Stop Treatment of Percutaneous LAAO Combined with Coronary Intervention for Higher Risk of Bleeding in Patients with AF Complicated with CHD.

Heart Surg Forum 2021 May 25;24(3):E474-E478. Epub 2021 May 25.

Department of Cardiology, Zhuhai People's Hospital (Zhuhai Hospital affiliated with Jinan University), Zhuhai, China.

Aims: To explore the feasibility, safety, and efficacy of 1-stop treatment of percutaneous left atrial appendage occlusion (LAAO) combined with coronary intervention for patients with nonvalvular atrial fibrillation (AF) complicated with coronary heart disease (CHD).

Methods And Results: We retrospectively analyzed the clinical data of 6 patients with AF combined with CHD admitted from Zhuhai People's Hospital from April 2017 to June 2018. After the operation, all patients were treated with aspirin (100 mg qd) and clopidogrel (75 mg qd) for 1 year, which is considered long-term use of aspirin/clopidogrel. The effects of LAAO and coronary intervention were evaluated immediately. The location of the left atrial appendage occluder, thrombosis, residual leak, and clinical manifestations were observed during the 90-day follow-up. The patients were implanted with Watchman™ devices and coronary stents. After the operation, the immediate sealing effect was satisfactory. The Watchman occluder was used in accordance with the PASS principle (position, anchor, size, seal), and the coronary intervention was satisfactory. During the operation, there were no device-related thrombosis, tamponade, or vascular complications. Follow-up results showed that in the 6 patients, there were no hemorrhagic strokes, worsening heart function, residual leakage, device-related thrombosis, angina pectoris, myocardial infarction, skin ecchymosis, gastrointestinal bleeding, or cerebral hemorrhage.

Conclusion: For patients with nonvalvular AF combined with CHD, the safety and feasibility of 1-stop treatment with left atrial appendage and coronary intervention are reliable, and the curative effects were also satisfactory at short- and medium-term follow-up.
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http://dx.doi.org/10.1532/hsf.3701DOI Listing
May 2021

Comparability of thyroid-stimulating hormone immunoassays using fresh frozen human sera and external quality assessment data.

PLoS One 2021 15;16(6):e0253324. Epub 2021 Jun 15.

Department of Clinical Laboratory, Beijing Chaoyang Hospital, Beijing Center for Clinical Laboratories, The Third Clinical Medical College of Capital Medical University, Beijing, P.R. China.

Background: This study aimed to assess the comparability among assays using freshly frozen human sera and external quality assessment (EQA) data in China.

Methods: Twenty-nine serum samples and two commercial EQA materials, obtained from the National Center for Clinical Laboratories (NCCL), were analyzed in triplicate using eight routine TSH assays. The commutability of commercial EQA materials (NCCL materials) was evaluated in accordance with the CLSI EP30-A and IFCC bias analysis. Median values obtained for the NCCL EQA materials were used to determine the systematic and commutability-related biases among immunoassays through back-calculation. The comparability of TSH measurements from a panel of clinical samples and NCCL EQA data was determined on the basis of Passing-Bablok regression. Furthermore, human serum pools were used to perform commutable EQA.

Results: NCCL EQA materials displayed commutability among three or five of seven assay combinations according CLSI or IFCC approach, respectively. The mean of systematic bias ranged from -13.78% to 9.85% for the eight routine TSH assays. After correcting for systematic bias, averaged commutability-related biases ranged between -42.26% and 12.19%. After correction for systematic and commutability -related biases, the slopes indicating interassay relatedness ranged from 0.801 to 1.299 using individual human sera, from 0.735 to 1.254 using NCCL EQA data, and from 0.729 to 1.115 using pooled human serum EQA(the commutable EQA).

Conclusions: The harmonization of TSH measurement is challenging; hence, systematic and commutability-related biases should be determined and corrected for accurate comparisons among assays when using human individual serum and the commercial EQA materials.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253324PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205121PMC
June 2021

The Use of Magnesium Sulfate and Peripheral Nerve Blocks: An Updated Meta-analysis and Systematic Review.

Clin J Pain 2021 Aug;37(8):629-637

Department of Anesthesiology, The First Affiliated Hospital of Xiamen University, Xiamen, China.

Objectives: With the popularization of ultrasound, nerve blocks have been widely implemented in current clinical practice. Although, they have seen limited success due to their shorter duration and suboptimal analgesia. Magnesium sulfate as a local anesthetic adjuvant for peripheral nerve blocks could enhance the effects of local anesthetics. However, previous investigations have not thoroughly investigated the analgesic efficacy of magnesium sulfate as an adjunct to local anesthetics for peripheral nerve blocks. Thus, we attempted to fill the gap in the existing literature by conducting a meta-analysis.

Materials And Methods: We performed of a quantitative systematic review of randomized controlled trials published between May 30, 2011 and November 1, 2018. Inclusion criteria were: (1) magnesium sulfate used as adjuvant mixed with local anesthetic for nerve blockade, (2) complete articles and published abstracts of randomized controlled trials, (3) English language.

Primary And Secondary Outcome Measures: The primary outcome measures were time of onset, total duration of the sensory blockade, and Visual Analog Scale pain scores. The secondary outcomes were postoperative oral and intravenous analgesics consumption and the incidence of nausea and vomiting.

Results: The 21 trials analyzed in this study included 1323 patients. Magnesium sulfate effectively prolonged the total duration of sensory blockade (mean difference [MD]=114.59 min, 95% confidence interval [CI]: 89.31-139.88), reducing Visual Analog Scale pain scores at 6 hours (MD=1.36 points, 95% CI: -2.09 to -0.63) and 12 hours (MD=1.54 points, 95% CI: -2.56 to -0.53) postsurgery. Magnesium sulfate also effectively reduced postoperative analgesic use within 24 hours postsurgery (standard MD=-2.06, 95% CI: -2.67 to -1.35). Furthermore, adjuvant magnesium sulfate significantly reduced the incidence of nausea and vomiting after transversus abdominis plane blockade (odds ratio: 0.39, 95% CI: 0.18-0.81).

Conclusion: Adjuvant magnesium sulfate enhanced the anesthetic effects of local anesthetics and improved postoperative analgesia following the perineural blockade.
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http://dx.doi.org/10.1097/AJP.0000000000000944DOI Listing
August 2021

A coordinated ruthenium-rifampicin complex reprogramming the colon carcinoma micro-environment mediated by modulation of p53/AkT/mTOR/VEGF pathway.

Toxicol Appl Pharmacol 2021 09 11;426:115618. Epub 2021 Jun 11.

Department of Cancer Center, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, Sichuan 610072, China. Electronic address:

WHO suggests that colon cancer incidences are rising steadily, propelling researchers to search for novel chemotherapeutic options. Metal-based chemotherapy is a potential forte to explore ruthenium-based complexes, exhibiting the capability to influence a variety of cellular targets. We discovered the chemotherapeutic effects of ruthenium-rifampicin complex on HT-29 and HCT-116 human colorectal cell lines and on a chemically developed murine colorectal cancer model. Complex was synthesized and characterized by analytical techniques and evaluation of antioxidant potential along with DNA binding capabilities. The complex minimizes cellular propagation and initiates apoptotic events in the colon cancer cell lines of HT-29 and HCT-116. The results of the in vivo study suggest that the complex has been successful in minimizing the wide spectrum of aberrant crypt foci and hyperplastic lesions, as well as encouraging elevated amounts of CAT, SOD and glutathione. Along with that, p53 could be modulated by the ruthenium-rifampicin complex to interfere with apoptosis in colon carcinoma, initiated by the intrinsic apoptotic trail facilitated through Bcl2 and Bax, thus controlling the Akt/mTOR/VEGF pathway coupled through the WNT/β-catenin trail. Ruthenium-rifampicin chemotherapy could interrupt, retract or interrupt the progression of colorectal cancer through modifying intrinsic apoptosis including the antiangiogenic pathway, thereby achieving the function of a potential contender in chemotherapy in the near future.
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http://dx.doi.org/10.1016/j.taap.2021.115618DOI Listing
September 2021

RNAi of vacuolar-type H-ATPase genes causes growth delay and molting defect in .

Bull Entomol Res 2021 Jun 11:1-10. Epub 2021 Jun 11.

Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing210095, China.

Henosepilachna vigintioctopunctata is one of the most serious insect pests to a large number of nightshades and cucurbits. RNA interference (RNAi) triggered by double-stranded RNA (dsRNA) offers a reduced risk approach to control the beetle. Identification of amenable target genes and determination of appropriate life stage for dsRNA treatment are two critical steps in order to improve RNAi efficiency. In the present paper, we identified three vATPase genes, namely HvvATPaseC, HvvATPaseE and HvvATPaseH. We found that the three transcripts were widely expressed in the eggs, first- to fourth-instar larvae, prepupae, pupae and adults. They were abundantly transcribed in the hindgut and Malpighian tubules, in contrast to the epidermis and fat body. Three days' ingestion of dsvATPaseC, dsvATPaseE and dsvATPaseH by the fourth-instar larvae significantly decreased corresponding transcript level by 90.1, 88.9 and 97.2%, greatly reduced larval fresh weight by 28.0, 29.9 and 28.0%, and caused 66.7, 100 and 78.7% larval lethality respectively. Comparably, 3 days' exposure of the third-instar larvae to dsvATPaseC significantly reduced HvvATPaseC mRNA level by 89.5%, decreased approximately 80% of the larval fresh weight, and killed 100% of the treated larvae. Therefore, the three vATPase genes, especially HvvATPaseE, are potential amenable target genes and young larvae are more susceptible to dsRNA. Our findings will enable the development of the dsRNA-based pesticide to control H. vigintioctopunctata.
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http://dx.doi.org/10.1017/S0007485321000420DOI Listing
June 2021

Dissolved iron and isotopic geochemical characteristics in a typical tropical river across the floodplain: The potential environmental implication.

Environ Res 2021 Jun 7;200:111452. Epub 2021 Jun 7.

Nu Instruments, 74 Clywedog Road South, Wrexham Industrial Estate, Wrexham, LL13 9XS, United Kingdom.

Iron (Fe) is an essential element for bio-physiological functioning terrestrial organisms, in particular of aquatic organisms. It is therefore crucial to understand the aquatic iron cycle and geochemical characteristics, which is also significant to obtain the key information on earth-surface evolution. The stable iron isotopic composition (δFe) of the dissolved fraction is determined in the Mun River (main tributary of Mekong River), northeast Thailand to distinguish the human and nature influenced riverine iron geochemical behavior. The results show that dissolved Fe concentration ranges from 8.04 to 135.27 μg/L, and the δFe ranges from -1.34‰ to 0.48‰, with an average of 0.23‰, 0.14‰ and -0.15‰ in the upper, middle and lower reaches, respectively. The δFe values of river water are close to that of the bulk continental crust and other tropical rivers. The correlations between δFe and Fe, Al, and physicochemical parameters show mixing processes of different Fe end-members, including the rock weathering end-member (low Fe/Al ratio and high δFe), the urban activities end-member (high Fe/Al ratio and moderate δFe), and a third end-member with probable sources from the Chi River and reservoir. For the most river water samples, the primary contribution is attributed to rock weathering, and the second is urban activities (only a few samples are from the upper and middle reaches). Thus, Fe isotopes could be employed as a proxy to identify and quantify the natural and anthropogenic contributions, respectively. These findings also provide data support for the scientific management of water resources in the Mun River catchment and other large tropical rivers.
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http://dx.doi.org/10.1016/j.envres.2021.111452DOI Listing
June 2021

Elevated HIV Infection of CD4 T Cells in MRKAd5 Vaccine Recipients Due to CD8 T Cells Targeting Adapted Epitopes.

J Virol 2021 Jul 26;95(16):e0016021. Epub 2021 Jul 26.

Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA.

HIV frequently escapes CD8 T cell responses, leading to the accumulation of viral adaptations. We recently demonstrated that during chronic HIV infection, adapted epitopes can promote maturation of dendritic cells (DCs) through direct CD8 T cell interactions and lead to enhanced HIV -infection of CD4 T cells. Here, we sought to determine the role of such adaptations following HIV MRKAd5 vaccination. We observed that vaccine-induced adapted epitope-specific CD8 T cells promoted higher levels of DC maturation than nonadapted ones and that these matured DCs significantly enhanced HIV -infection. These matured DCs were associated with higher levels of interleukin 5 (IL-5) and IL-13 and a lower level of CXCL5, which have been shown to impact DC maturation, as well as a lower level of CXCL16. Finally, we observed that vaccine recipients with high HLA-I-associated adaptation became HIV infected more quickly. Our results offer another possible mechanism for enhanced infection among MRKAd5 vaccinees. Despite the well-established contribution of CD8 T cells in HIV control, prior CD8 T cell-based HIV vaccines have failed to demonstrate any efficacy in preventing viral infection. One such vaccine, known as the MRKAd5 vaccine, showed a potential increased risk of viral infection among vaccine recipients. However, the underlying mechanism(s) remains unclear. In this study, we observed that vaccine recipients with high adaptation to their HLA-I alleles were associated with an increased HIV infection risk in comparison to the others. Similar to what we observed in HIV infection in the prior study, adapted epitope-specific CD8 T cells obtained from vaccine recipients exhibit a greater capacity in facilitating viral infection by promoting dendritic cell maturation. Our findings provide a possible explanation for the enhanced viral acquisition risk among MRKAd5 vaccine recipients and highlight the importance of optimizing vaccine design with consideration of HLA-I-associated HIV adaptation.
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http://dx.doi.org/10.1128/JVI.00160-21DOI Listing
July 2021

Copper-Based Plasmonic Catalysis: Recent Advances and Future Perspectives.

Adv Mater 2021 May 29:e2008145. Epub 2021 May 29.

Hefei National Laboratory for Physical Sciences at the Microscale, Key Laboratory of Strongly-Coupled Quantum Matter Physics of Chinese Academy of Sciences, Key Laboratory of Surface and Interface Chemistry and Energy Catalysis of Anhui Higher Education Institutes, Department of Chemical Physics, University of Science and Technology of China, Hefei, Anhui, 230026, P. R. China.

With the capability of inducing intense electromagnetic field, energetic charge carriers, and photothermal effect, plasmonic metals provide a unique opportunity for efficient light utilization and chemical transformation. Earth-abundant low-cost Cu possesses intense and tunable localized surface plasmon resonance from ultraviolet-visible to near infrared region. Moreover, Cu essentially exhibits remarkable catalytic performance toward various reactions owing to its intriguing physical and chemical properties. Coupling with light-harvesting ability and catalytic function, plasmonic Cu serves as a promising platform for efficient light-driven chemical reaction. Herein, recent advancements of Cu-based plasmonic photocatalysis are systematically summarized, including designing and synthetic strategies for Cu-based catalysts, plasmonic catalytic performance, and mechanistic understanding over Cu-based plasmonic catalysts. What's more, approaches for the enhancement of light utilization efficiency and construction of active centers on Cu-based plasmonic catalysts are highlighted and discussed in detail, such as morphology and size control, regulation of electronic structure, defect and strain engineering, etc. Remaining challenges and future perspectives for further development of Cu-based plasmonic catalysis are also proposed.
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http://dx.doi.org/10.1002/adma.202008145DOI Listing
May 2021

Optimization of electrocoagulation process parameters for enhancing phosphate removal in a biofilm-electrocoagulation system.

Water Sci Technol 2021 May;83(10):2560-2574

Tianjin Water Engineering Co., Ltd, Tianjin, 300222, China.

This study aimed to enhance the removal of phosphate in synthetic rural sewage by using a continuous electrocoagulation (EC) combined with biofilm process in an integrated system. Characteristic indexes of biofilm process effluent covering pH, dissolved oxygen (DO), suspended solids (SS), chemical oxygen demand (COD) and phosphate maintained a narrow fluctuation range and tended not readily to influence the phosphate removal of subsequent electrocoagulation. Three parameters including inter-electrode distance, current intensity and reaction time were selected to investigate the performance of enhancing phosphate removal. On the strength of single-factor tests, the Box-Behnken design (BBD) coupled with response surface methodology (RSM) was applied to investigate the individual and mutual interaction impacts of the major operating parameters and to optimize conditions. The optimum conditions were found to be inter-electrode distance of 1.8 cm, current density of 2.1 mA/cm and EC reaction time of 34 min, and phosphate removal efficiency of 90.24% was achieved along with less than 1 mg/L in case of periodic polarity switching mode, which raised removal efficiency by 10.10% and reduced operating cost by 0.13 CNY/g PO compared to non-switching mode. The combination of biofilm processing and electrocoagulation treatment was proven to be a valid and feasible method for enhancing phosphate removal.
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http://dx.doi.org/10.2166/wst.2021.132DOI Listing
May 2021

Generated High-Valent Iron Single-Atom Catalyst for Efficient Oxygen Evolution.

Nano Lett 2021 Jun 21;21(11):4795-4801. Epub 2021 May 21.

Hefei National Laboratory for Physical Sciences at the Microscale, Key Laboratory of Strongly-Coupled Quantum Matter Physics of Chinese Academy of Sciences, National Synchrotron Radiation Laboratory, Key Laboratory of Surface and Interface Chemistry and Energy Catalysis of Anhui Higher Education Institutes, Department of Chemical Physics, University of Science and Technology of China, Hefei, Anhui 230026, P. R. China.

Oxygen evolution reaction (OER) plays an important role in renewable energy supplies as the anodic reaction for electrochemical transformation of various chemicals. Iron-based OER catalysts are potential candidates due to their abundance but suffer from poor activity. Here we demonstrate that a single-atom iron catalyst with - generated Fe centers is highly active toward OER. Only an overpotential of 320 mV was needed to reach 10 mA cm. The catalyst exhibited an ultrahigh turnover frequency of 0.62 s at an overpotential of 0.35 V, which is comparable to currently reported transitional-metal based OER catalysts. Experimental and theoretical studies revealed that the valence state of the metal center transferred from Fe to highly active Fe prior to the OER process. This transformation was originated from the strong interaction between atomic Fe and carbon support via C-O-Fe bonding, leading to a lower energy barrier of the rate-limiting *OOH formation.
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http://dx.doi.org/10.1021/acs.nanolett.1c01335DOI Listing
June 2021

Tuning the hyperconjugative aromaticity in Au(III)-substituted indoliums.

Dalton Trans 2021 Jun;50(23):8096-8101

State Key Laboratory of Physical Chemistry of Solid Surfaces and Collaborative Innovation Center of Chemistry for Energy Materials (iChEM), Fujian Provincial Key Laboratory of Theoretical and Computational Chemistry and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

As a fundamental concept in chemistry, aromaticity has been extended from traditional organics to organometallics. Similarly, hyperconjugative aromaticity (HCA) has also been developed from main group to transition metal systems through the hyperconjugation of the substituents. However, it remains unclear that how the oxidation state of transition metal in the substituents affects the HCA. Herein, we demonstrate via density functional theory calculations that HCA could disappear in indoliums when the Au(i) substituents are changed to the Au(iii) ones. By tuning the ligand or cis-trans isomerization, HCA could be regained or enhanced in indoliums containing Au(iii) substitutents.
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http://dx.doi.org/10.1039/d1dt00599eDOI Listing
June 2021

Rainwater chemistry observation in a karst city: variations, influence factors, sources and potential environmental effects.

Authors:
Jie Zeng Guilin Han

PeerJ 2021 20;9:e11167. Epub 2021 Apr 20.

Institute of Earth Sciences, China University of Geosciences (Beijing), Beijing, China.

The rainwater chemistry and related air contaminants are used to investigate the rainwater ions sources, variations, and influence factors from 2012 to 2014 in Guiyang city (the typical karst urban area of Southwest China). According to temporal rainwater ion concentrations, the obvious variations were presented in the study period, such as Ca (125∼6,652 μeq L) and SO (11∼4,127 μeq L). Consequently, Ca, Mg, SO and Cl are considered as the leading ions. Three critical influencing factors of rainwater ions concentrations, including sources variations, rainfall amount and long-distance migration (rainfall amount > 100 mm) are identified. Based on the typical ionic ratios, source identification suggested that anthropogenic inputs mainly contributed to F, NO and SO , while the dusts (crustal sources) are the primary sources of Mg, Ca and K. Cl Enrichment in long-distance transport is the main contributor of Cl. According to the observation of high level of total wet acid deposition, the more detailed spatio-temporal monitoring of rainfall-related acid deposition (particularly sulfur deposition) is required to understand its potential environmental effects in the aquatic ecosystem of the earth surface.
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http://dx.doi.org/10.7717/peerj.11167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065247PMC
April 2021

Viscoelasticity measured by shear wave elastography in a rat model of nonalcoholic fatty liver disease: comparison with dynamic mechanical analysis.

Biomed Eng Online 2021 May 3;20(1):45. Epub 2021 May 3.

School of Biomedical Engineering, Health Science Center, Shenzhen University, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Shenzhen, 518000, China.

Background: Nonalcoholic fatty liver disease (NAFLD) is rapidly becoming one of the most common liver diseases. Ultrasound elastography has been used for the diagnosis of NAFLD. However, clinical research on steatosis by elastography technology has mainly focused on steatosis with fibrosis or non-alcoholic steatohepatitis (NASH), while steatosis without fibrosis has been poorly studied. Moreover, the relationship between liver viscoelasticity and steatosis grade is not clear. In this study, we evaluated the degree of liver steatosis in a simple steatosis rat model using shear wave elastography (SWE).

Results: The viscoelasticity values of 69 rats with hepatic steatosis were measured quantitatively by SWE in vivo and validated by a dynamic mechanical analysis (DMA) test. Pathological sections were used to determine the steatosis grade for each rat. The results showed that the elasticity values µ obtained by the two methods followed the same trend, and µ is significantly correlated with liver steatosis. The Pearson's correlation coefficients indicate that [Formula: see text] obtained by SWE is positively linear correlated with DMA (r = 0.628, p = 7.85 × 10). However, the viscosity values [Formula: see text] obtained by SWE were relatively independent of those obtained by DMA with a correlation coefficient of - 0.01. The combined Voigt elasticity measurements have high validity in the prediction of steatosis (S0 vs. S1-S4), with an AUROC of 0.755 (95% CI 0.6175-0.8925, p < 0.01) and the optimal cutoff value was 2.08 kPa with a sensitivity of 78% and specificity of 63%.

Conclusion: SWE might have the feasibility to be introduced as an auxiliary technique for NAFLD patients in clinical settings. However, the viscosity results measured by SWE and DMA are significantly different, because the two methods work in different frequency bands.
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http://dx.doi.org/10.1186/s12938-021-00879-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091696PMC
May 2021

Sequential Superassembly of Nanofiber Arrays to Carbonaceous Ordered Mesoporous Nanowires and Their Heterostructure Membranes for Osmotic Energy Conversion.

J Am Chem Soc 2021 May 30;143(18):6922-6932. Epub 2021 Apr 30.

Department of Chemistry, Shanghai Key Lab of Molecular Catalysis and Innovative Materials, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, Shanghai 200438, P. R. China.

The capture of sustainable energy from a salinity gradient, in particular, using renewable biomass-derived functional materials, has attracted significant attention. In order to convert osmotic energy to electricity, many membrane materials with nanofluidic channels have been developed. However, the high cost, complex preparation process, and low output power density still restrict the practical application of traditional membranes. Herein, we report the synthesis of highly flexible and mechanically robust nanofiber-arrays-based carbonaceous ordered mesoporous nanowires (CMWs) through a simple and straightforward soft-templating hydrothermal carbonization approach. This sequential superassembly strategy shows a high yield and great versatility in controlling the dimensions of CMWs with the aspect ratio changes from about 3 to 39. Furthermore, these CMWs can be used as novel building blocks to construct functional hybrid membranes on macroporous alumina. This nanofluidic membrane with asymmetric geometry and charge polarity exhibits low resistance and high-performance energy conversion. This work opens a solution-based route for the one-pot preparation of CMWs and functional heterostructure membranes for various applications.
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http://dx.doi.org/10.1021/jacs.1c00547DOI Listing
May 2021

[Sources and Fate of Nitrate in Groundwater in a Typical Karst Basin: Insights from Carbon, Nitrogen, and Oxygen Isotopes].

Huan Jing Ke Xue 2021 May;42(5):2268-2275

Institute of Karst Geology, Chinese Academy of Geological Sciences, Guilin 541004, China.

Multiple isotopes (C, N, and O) and hydrochemical data were used to trace the sources and fate of nitrate in ground and surface waters of the Babu subterranean stream watershed in Guizhou Province. The origin of nitrate in the water samples was also quantitatively analyzed by the SIAR model. Approximately 38% of the groundwater samples were not drinkable because the nitrate exceeded the drinking water standard, thereby indicating that the groundwater was seriously polluted by nitrate. The ranges of N-NO, O-NO, and O-HO in groundwater were 2.30‰-30.33‰ (mean of 9.68‰), 2.65‰-13.73‰ (mean of 6.64‰), and -8.83‰﹣-7.37‰ (mean of -8.18‰), respectively. Based on the stable isotopic compositions (N-NO, O-NO, and O-HO), nitrification was the dominant process in the basin. The nitric acid produced by nitrification promoted the dissolution of carbonate rocks, thereby leading to a significantly negative correlation (<0.001) between the carbon isotope of dissolved inorganic carbon (C) and N-NO and indicating that C, combined with N-NO, is effective in exploring the fate of nitrate in karst groundwater. The nitrate in the ground and surface waters mainly originated from soil N, manure and sewage, and ammonium nitrogen fertilizer. The results of the SIAR model showed that the contributions of soil N, manure and sewage, and ammonium nitrogen fertilizer were 36.19%, 33.71%, and 30.10% in groundwater, respectively, and 39.15%, 36.08%, and 24.77% in surface water, respectively. Therefore, it would be more effective to reduce the nitrate recharge flux in groundwater by simultaneously removing nitrate and ammonium nitrogen during wastewater treatment and by adopting scientific fertilization technology in agricultural areas.
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http://dx.doi.org/10.13227/j.hjkx.202010028DOI Listing
May 2021

Ligand-Mediated Spatially Controllable Superassembly of Asymmetric Hollow Nanotadpoles with Fine-Tunable Cavity as Smart HO-Sensitive Nanoswimmers.

ACS Nano 2021 Apr 16. Epub 2021 Apr 16.

Department of Chemistry, Shanghai Key Lab of Molecular Catalysis and Innovative Materials, iChEM, Fudan University, Shanghai 200438, China.

Ligand-mediated interface control has been broadly applied as a powerful tool in constructing sophisticated nanocomposites. However, the resultant morphologies are usually limited to solid structures. Now, a facile spatially controllable ligand-mediated superassembly strategy is explored to construct monodispersed, asymmetric, hollow, open Au-silica (SiO) nanotadpoles (AHOASTs). By manipulating the spatial density of ligands, the degree of diffusion of silica can be precisely modulated; thus the diameters of the cavity can be continuously tuned. Due to their highly anisotropic, hollow, open morphologies, we construct a multicompartment nanocontainer with enzymes held and isolated inside the cavity. Furthermore, the resulting enzyme-AHOASTs are used as biocompatible smart HO-sensitive nanoswimmers and demonstrate a higher diffusion coefficient than other nanoscaled swimmers. We believe that this strategy is critical not only in designing sophisticated hollow nanosystem but also in providing great opportunities for applications in nanomaterial assembly, catalysis, sensors, and nanoreactors.
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http://dx.doi.org/10.1021/acsnano.1c01159DOI Listing
April 2021

Effect of different factors on treatment of oily wastewater by TiO/AlO-PVDF ultrafiltration membrane.

Environ Technol 2021 Apr 18:1-9. Epub 2021 Apr 18.

School of Environmental Science and Engineering, Hainan University, Haikou, People's Republic of China.

An ultrafiltration membrane developed by our research group was applied to treat simulated emulsified oil wastewater. ATR-FTIR, SEM, TEM, and Zeta potential analyzes demonstrated that the modified ultrafiltration membrane (MM) has excellent stability and anti-fouling capacity than origin membrane (OM), which possesses a pure water flux of 260 L·m·h and oil/water (o/w) rejection of 98.5 ± 0.33%. Inorganic salt CaCl has more considerable influence than MgSO and NaCl under the same mass concentration in the two membranes UF process. Along with concentration increasing, flux sharply reduces; meanwhile, the rejection has an opposite trend. Moreover, permeation flux has a maximum value, and the rejection also gets its optimal state under neutral conditions during the pH value of 2-12. The membrane also exhibits excellent anti-fouling performance and anti- o/w adsorption properties with an adsorption rate below 0.8% compared with OM, which has an adsorption rate of nearly 2.1%, respectively. A kind of new UF membrane developed by our research group was applied to treat simulated o/w. ATR-FTIR, SEM, TEM, and Zeta potential analyzes demonstrated that PVDF-AlO/TiO material has excellent stability and anti-fouling capacity. CaCl has the greatest influence than MgSO and NaCl under the same mass concentration. Moreover, permeation flux has maximum value and the rejection also gets its optimal state under neutral conditions during pH 2-12. The membrane also exhibits excellent anti-fouling performance and anti-O/W adsorption properties with adsorption rate below 0.8% compared with OM which has an adsorption rate nearly 2.1%, respectively.
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http://dx.doi.org/10.1080/09593330.2021.1912832DOI Listing
April 2021

Development of a designated comparison method for alkaline phosphatase measurements and its application to evaluating routine methods.

Scand J Clin Lab Invest 2021 May 23;81(3):218-224. Epub 2021 Mar 23.

Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, National Center for Clinical Laboratories, Beijing Engineering Research Center of Laboratory Medicine, Beijing, China.

The International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) published the reference measurement procedure (RMP) for ALP measurement in 2011. However, the RMP is of high requirements for laboratories, complicated, time-consuming and high cost of reagents. Many manufacturers do not trace results to the higher procedure. And there is currently no designated comparison method (DCM) for ALP measurement. Thus, the standardization of ALP measurement is hindered. Automatic biochemical analyzers are easy to operate and widely used in clinical laboratories. Therefore, according to the RMP, establishing a DCM based on an automatic biochemical analyzer will be a practical way to establish traceability to the accuracy base and promote the standardization of ALP measurement. On the basis of conforming to the RMP recommended by IFCC as far as possible, the DCM was established based on a Thermo Indiko automatic biochemical analyzer. Performances of the method were validated. The DCM repeatability and within laboratory imprecision was <1% and <2.5%, respectively. For evaluation of trueness, the biases were within the equivalent limits. Measurement procedure comparisons and biases estimation were carried out between the DCM, the RMP, and the six routine methods using a panel of 40 individual human serum samples. The comparisons between the DCM and the RMP gave satisfying results. Compared with the DCM, the relative biases of some routine methods failed to meet the bias limit derived from biological variation.
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http://dx.doi.org/10.1080/00365513.2021.1901305DOI Listing
May 2021

Theaflavin alleviates oxidative injury and atherosclerosis progress via activating microRNA-24-mediated Nrf2/HO-1 signal.

Phytother Res 2021 Jun 23;35(6):3418-3427. Epub 2021 Mar 23.

College of Food Science, Southwest University, Chongqing, China.

Theaflavin (TF) in black tea has been shown to have significant antioxidant and anti-inflammatory capacity; however, the effects and the underlying mechanism of TF on atherosclerosis (AS) remain unclear. Herein, we investigated the effects and the potential mechanism of TF on AS progression in vivo and in vitro. ApoE mice were administrated with high fat diet (HFD) or HFD + TF (5 or 10 mg, i.g.) for 12 weeks. The results indicated that TF administration effectively decreases the serum lipid levels and the production of MDA in HFD-fed mice. Meanwhile, TF promotes the activities of antioxidant enzymes (SOD, CAT, and GSH-Px) and inhibits the formation of atherosclerotic plaque and the process of histological alterations in the aorta. In vitro, TF pretreatment could protect against cholesterol-induced oxidative injuries in HUVEC cells, decreasing the level of ROS and MDA, maintaining the activities of antioxidant enzymes. Further study revealed that TF upregulates Nrf2/HO-1 signaling pathway in vascular endothelial cells. Moreover, TF increases the level of microRNA-24 (miR-24), and miR-24 inhibition markedly compromises TF-induced Nrf2 activation and protective effects. In conclusion, the present study indicated that theaflavins may achieve the anti-atherosclerotic effect via activating miR-24-mediated Nrf2/HO-1 signaling pathway.
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http://dx.doi.org/10.1002/ptr.7064DOI Listing
June 2021
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