Publications by authors named "Jie Yu"

1,538 Publications

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The in-situ effect of HS on the decomposition of natural rubber and catalyst activity.

Chemosphere 2021 Jun 18;283:131252. Epub 2021 Jun 18.

State Key Laboratory of Coal Combustion, Huazhong University of Science and Technology, 430074, Wuhan, Hubei, China.

In this work, the pyrolysis of natural rubber (NR) under N and HS atmosphere was performed to illustrate the possible effect of HS on NR decomposition with and without catalysts. A molecular dynamics simulation based on reactive force field (ReaxFF) was also conducted to understand the interaction mechanism between HS and intermediates from NR decomposition. Furthermore, the catalytic decomposition of NR under HS atmosphere and the adsorption characteristics of HS by zeolites alone were also carried out to investigate the effect of catalysts on sulfur behavior and the reversed effect of HS on catalyst activity. This work revealed that the introduction of HS can influence the yields of pyrolytic oil and gas, as well as composition of the oil. Combining experimental and simulation studies, HS can interact with intermediates from NR decomposition forming sulfur-containing substances in pyrolytic oil. The HS adsorption experiments by various catalysts revealed that catalysts can chemically adsorb HS. The introduction of Zn can promote the adsorption ability by reacting with sulfur-containing substances to generate ZnS, with the desulfurization effect following the order of 3Zn/ZSM5 > ZSM5 > 3ZnO/ZSM5.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131252DOI Listing
June 2021

Black Tea Quality is Highly Affected during Processing by its Leaf Surface Microbiome.

J Agric Food Chem 2021 Jun 21. Epub 2021 Jun 21.

State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China.

Microbiomes can greatly affect the quality of fermented food and beverages, including tea. In this study, microbial populations were characterized during black and green tea manufacturing, revealing that tea processing steps can drive both the bacterial and fungal community structure. Tea leaves were found to mostly harbor Proteobacteria, Bacteriodetes, Firmicutes, and Actinobacteria among bacteria and Ascomycetes among fungi. During processing, tea microbial populations changed especially between sterilized and unsterilized samples. The surface sterilization of fresh leaves before processing can remove many microbes, especially the bacteria of the genera and , indicating that these are mostly phylloplane microbes on tea leaves. The surface sterilization removed most fungi, except the . We also observed a fluctuation in the content of several tea quality-related metabolites during processing. Caffeine and theanine were found in the same quantities in green tea with or without leaf surface sterilization. However, the sterilization process dramatically decreased the content of total catechins and theanine in black tea, indicating that microbes on the surface of tea leaf may be involved in maintaining the formation of these important metabolites during black tea processing.
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http://dx.doi.org/10.1021/acs.jafc.1c01607DOI Listing
June 2021

Aligned Millineedle Arrays for Solar Power Seawater Desalination with Site-Specific Salt Formation.

Small 2021 Jun 21:e2101487. Epub 2021 Jun 21.

Center of Super-Diamond and Advanced Films (COSDAF) and Department of Chemistry, City University of Hong Kong, Hong Kong, SAR, 999077, P. R. China.

As a sustainable and clean water production technology, solar thermal water evaporation has been extensively studied in the past few years. One challenge is that upon operation, salt would form on surface of the solar absorbers leading to inefficient water supply and light absorption and thus much reduced water vaporization rate. To address this problem, a simple solar evaporator based on an array of aligned millineedles for efficient solar water evaporation and controlled site-specific salt formation is demonstrated. The maximum solar evaporation rate achieved is 2.94 kg m h under one Sun irradiation in brine of high salinity (25 wt% NaCl), achieving energy conversion efficiency of 94.5% simultaneously. More importantly, the spontaneously site-specific salt formation on the tips of millineedles endows this solar evaporator with salt harvesting capacity. Rationally separating the clean water and salt from brine by condensation and gravity assistance, this tip-preferential crystallization solar evaporator is not affected by the salt clogging compared with conventional 2D solar evaporators. This study provides new insights on the design of solar evaporators and advances their applications in sustainable seawater desalination and wastewater management.
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http://dx.doi.org/10.1002/smll.202101487DOI Listing
June 2021

Comparative proteomic analysis of seminal plasma proteins in relation to freezability of Dezhou donkey semen.

Anim Reprod Sci 2021 Jun 11;231:106794. Epub 2021 Jun 11.

National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., No. 78, E-jiao Street, Dong'e Country, Shandong Province, 252201, China. Electronic address:

Variation in donkey sperm freezability (capacity to withstand freeze-thawing) between ejaculates is a limitation for sperm cryopreservation. Seminal plasma proteins are essential for sperm function and also related to individual differences in sperm freezability. A Tandem Mass Tag (TMT) peptide labeling combine with a LC-MS/MS approach was conducted to quantitatively identify the seminal plasma proteins differentially abundant in ejaculates with optimal freezability characteristics (GFE) compared with those with suboptimal freezability characteristics (PFE). A total of 866 proteins were identified, and 99 ejaculates were in larger abundance in GFE samples. Differentially abundant proteins (DAPs) were subjected to intensive bioinformatic analysis. The majority of DAPs were involved in metabolic processes, oxidation-reduction processes and biological regulation. Results from functional protein analysis suggested that proteins functioned in oxidoreductase activity and acid phosphatase activity. This is the first report where there were analyses of the proteome of seminal plasma from donkey ejaculates with different freezability and to identify candidate proteins that could be used to explore the molecular mechanism related to donkey sperm cryotolerance. In this study, there also was elucidation of biomarkers for the early identification and selection of donkeys with optimal semen freezability.
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http://dx.doi.org/10.1016/j.anireprosci.2021.106794DOI Listing
June 2021

Effect of Cedrus deodara extract on the physiochemical and sensory properties of salted meat and its action mechanism.

J Food Sci 2021 Jun 19. Epub 2021 Jun 19.

Antioxidant Polyphenols Team, Department of Food Engineering, Sichuan University, Chengdu, PR China.

The effect of pine needle extract from Cedrus deodara (PNE) on the quality of salted meat was reported, and its action mechanism was further investigated. With the treatment of PNE, the physicochemical properties of salted meat were improved. The peroxide value decreased from 16.18 to 6.78 mmol O /kg, while the thiobarbituric acid value decreased from 0.79 to 0.40 mg MDA/kg. Moreover, the salted meat with PNE also had the better texture, color, and volatile compositions. The 0.2% PNE group showed the highest ΔE value (63.16 ± 0.56), hardness (813.5 ± 48.7 g), and volatility (45.86 ± 0.39), while the control group showed the lowest ΔE value (43.92 ± 2.13), hardness (515.8 ± 17.3 g) and volatility (29.97 ± 0.56). In addition, with the analysis of fluorescence and circular dichroism spectroscopy, the spatial structures of myofibrillar protein (MP) in salted meat were obviously changed by PNE. Meanwhile, methylconiferin, 1-O-feruloyl-β-D-glucose, nortrachelogenin, secoxyloganin, 1-O-(4-coumaroyl)-β-D-glucose and pelargonidin-3-O-glucoside were identified from PNE. Furthermore, according to the analysis of molecular docking, hydrogen bond, hydrophobic force, and electrostatic force were obtained as the main molecular forces between MP and the phenolic compounds of PNE, while arginine, glutamic acid, and glycine residues were the main binding sites. All results suggested that PNE might be a potential candidate to improve the quality of salted meat in the food industry. PRACTICAL APPLICATION: The quality deterioration of meat may not only affect its further processing and consumption but also may lead to some food safety problems. In present study, PNE exhibited the fine capability to inhibit the oxidation of meat, while it could ameliorate the texture, color, and physicochemical properties of meat due to its tightly interaction with myofibrillar protein. All result suggested that PNE could be potentially utilized to improve the quality of meat in food industry.
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http://dx.doi.org/10.1111/1750-3841.15801DOI Listing
June 2021

Effect of infrared ray roasting on oxidation stability and flavor of virgin rapeseed oils.

J Food Sci 2021 Jun 19. Epub 2021 Jun 19.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.

Effects of infrared ray roasting (IRR) on the oxidation stability and flavors of virgin rapeseed oil (VROs) at 110-170°C were investigated and compared with traditional roller roasting (TRR). Results showed that IRR samples showed lower acid and peroxides values, higher oxidation stability index, and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity than TRR ones. IRR samples displayed better thermal expansion of rapeseed for internal fragmentation from microstructures, which facilitated the release of tocophenols (652.63-748.78 mg/kg) and 4-vinylsyringol (7.54-678.19 mg/kg), compared with TRR ones with tocophenols (652.63-689.28 mg/kg) and 4-vinylsyringol (7.54-524.18 mg/kg) contributing to better oxidation stability. Moreover, important volatile compounds, including pyrazines, isothiocyanates, nitriles and aldehydes, were formed quantitatively more in IRR than TRR samples, which was attributed to better heat transfer efficiency and internal fragmentation promoting complex reactions inside rapeseed. Therefore, IRR has more positive roasting effects on VROs than TRR. PRACTICAL APPLICATION: Virgin rapeseed oil is a massively consumed flavor vegetable oil, but the traditional high-temperature roller seed roasting process can cause serious quality problems. Our work applied a novel roasting technology, infrared ray roasting to rapeseed pretreatment. The results show that this new type of roasting technology is more efficient and stable and has important applications in the production of virgin rapeseed oil with better oxidative stability and flavor.
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http://dx.doi.org/10.1111/1750-3841.15792DOI Listing
June 2021

Design method for an off-axis reflective anamorphic optical system with aberration balance and constraint control.

Appl Opt 2021 Jun;60(16):4557-4566

This paper proposes a design method for an off-axis reflective anamorphic optical system (ORAOS). This method first applies vector aberration theory to establish a mathematical model to balance the aberration of an ORAOS. It then builds the error function of structural parameters and constraints through spatial ray tracing and grouping design. Next, it introduces automatic adjustment of weight factors for dynamic balance of aberrations and constraints. A particle swarm simulated annealing algorithm is used to iteratively calculate the initial structure of the ORAOS. Finally, we use an extreme ultraviolet (EUV) lithographic projection objective with off-axis six-reflective anamorphic mirrors (${\beta _x} = 1/4,{\beta _y} = 1/8$) as an example to verify the effectiveness of this method. We obtain an EUV lithographic anamorphic objective with a numerical aperture of 0.55 and a root mean square wavefront error better than $1/30\lambda$ (${\hat{\text I}}\gg\, = 13.5\;{\rm nm}$).
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http://dx.doi.org/10.1364/AO.427713DOI Listing
June 2021

[Clinical features and prognosis of pediatric acute megakaryocytic leukemia].

Zhongguo Dang Dai Er Ke Za Zhi 2021 Jun;23(6):613-620

Department of Hematology and Oncology, Children's Hospital of Chongqing Medical University/National Clinical Research Center for Child Health and Disorders/Ministry of Education Key Laboratory of Child Development and Disorders/Chongqing Key Laboratory of Pediatrics, Chongqing 401122, China.

Objective: To study the clinical features and prognosis of children with acute megakaryocytic leukemia (AMKL) and the clinical effect of acute myeloid leukemia 03 (AML03) regimen for the treatment of pediatric AMKL.

Methods: The clinical data were collected from 47 children with AMKL who were diagnosed from May 2011 to December 2019. The treatment outcomes and prognostic factors were analyzed. The Kaplan-Meier method and the log-rank test were used for survival analysis.

Results: Among the 47 children with AMKL, 22 with non-Down syndrome-AMKL were treated by the AML03 regimen, with a median follow-up time of 11.4 months. For the 22 non-Down syndrome-AMKL patients, the remission rate of bone marrow cytology was 85% and the negative rate of minimal residual disease (MRD) was 79% after induction Ⅱ, with a 2-year overall survival (OS) rate of (50±13)% and a 2-year event-free survival (EFS) rate of (40±12)%. The group with positive immunophenotypic marker CD56 had significantly lower 2-year EFS and OS rates than the group with negative CD56 ( < 0.05). The group without remission of bone marrow cytology after induction Ⅱ had significantly lower 2-year EFS and OS rates than the group with remission ( < 0.05). The group with positive MRD after induction Ⅱ had a significantly lower 2-year EFS rate than the group with negative MRD ( < 0.05). There was no significant difference in 2-year OS and EFS rates between the patients with transplantation and those without transplantation ( > 0.05).

Conclusions: Children with AMKL tend to have a low remission rate and a poor prognosis. Positive immunophenotypic marker CD56, bone marrow cytology during early treatment response, and MRD results are important factors influencing the prognosis. Allogeneic hematopoietic stem cell transplantation has no significant effect on the prognosis of AMKL.
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June 2021

Effects of seated lumbar rotation manipulation in treating degenerative lumbar instability: a protocol for a randomized controlled trial.

Trials 2021 Jun 14;22(1):398. Epub 2021 Jun 14.

Department of Spine, Wangjing Hospital of China Academy of Chinese Medical Sciences, Beijing, 100102, China.

Background: Degenerative lumbar instability (DLI) is a common disease that causes low back pain (LBP) in clinic. It is difficult to completely recover from DLI, and it occurs repeatedly, which seriously affects the quality of life of patients. The epidemiological survey showed that 20-30% of low back pain was related to lumbar instability. Increasing evidence shows that seated lumbar rotation manipulation can effectively improve the clinical symptoms of patients with low back pain. The primary aim of this clinical trial is to observe the intervention effect of seated lumbar rotation manipulation on DLI patients.

Method/design: A total of 60 participants with DLI will be recruited and randomly allocated into the seated lumbar rotation manipulation group (the intervention group) or lumbar traction in supine position group (the control group) in this prospective, outcome assessor-blind, two-arm randomized controlled clinical trial. The treatment of the two groups lasted for 3 weeks, and the manipulation of the intervention group would be carried out once every other day, three times a week, a total of 9 times; the control group would be given lumbar traction once a day, five times a week, a total of 15 times. JOA (Japanese Orthopaedic Association) and VAS (Visual Analogue Scales) scores will be recorded as the primary outcomes before the treatment and at the 1st, 3rd, 5th, 8th, 10th, 12th, 15th, 17th, and 19th days after treatment and follow-up visit at the first, third, and sixth months. JOA efficacy evaluation standard will be used to evaluate the overall efficacy as the secondary outcomes.

Discussion: The results of this prospective, randomized controlled trial will provide a clinical evidence for the treatment of DLI with seated lumbar rotation manipulation.

Trial Registration: Chinese Clinical Trial Registry ChiCTR2000032017 . Registered on 18 April 2020, Prospective registration.
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http://dx.doi.org/10.1186/s13063-021-05350-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204577PMC
June 2021

Efficacy Evaluation of 3D Navigational Template for Salter Osteotomy of DDH in Children.

Authors:
Jie Yu Qiang Shi

Biomed Res Int 2021 22;2021:8832617. Epub 2021 May 22.

Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.

Background: The aim of this study is to retrospectively evaluate the efficacy of 3D navigational template for Salter osteotomy of DDH in children.

Methods: Thirty-two consecutive patients with DDH who underwent Salter osteotomy were evaluated between July 2014 and August 2017, and they were divided into the conventional group ( = 16) and navigation template group ( = 16) according to different surgical methods. The corrective acetabular degrees, radiation exposure, and operation time were compared between the two groups.

Results: No nerve palsy or redislocation was reported in the navigation template group. Compared with the conventional group, the navigation template group had the advantages of more accurate acetabular degrees, less radiation exposure, and shorter operation time ( < 0.05). Meanwhile, the navigation template group achieved a better surgical outcome than the conventional group (McKay, = 0.0293; Severin, = 0.0949).

Conclusions: The 3D navigational template for Salter osteotomy of DDH is simple and effective, which could be an alternative approach to improve the Salter osteotomy accuracy and optimize the efficacy.
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http://dx.doi.org/10.1155/2021/8832617DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166498PMC
May 2021

Mechanisms of Neoantigen-Targeted Induction of Pyroptosis and Ferroptosis: From Basic Research to Clinical Applications.

Front Oncol 2021 27;11:685377. Epub 2021 May 27.

School of Basic Medicine Sciences, Weifang Medical University, Weifang, China.

Neoantigens are tumor-specific antigens (TSAs) that are only expressed in tumor cells. They are ideal targets enabling T cells to recognize tumor cells and stimulate a potent antitumor immune response. Pyroptosis and ferroptosis are newly discovered types of programmed cell death (PCD) that are different from apoptosis, cell necrosis, and autophagy. Studies of ferroptosis and pyroptosis of cancer cells are increasing, and strategies to modify the tumor microenvironment (TME) through ferroptosis to inhibit the occurrence and development of cancer, improve prognosis, and increase the survival rate are popular research topics. In addition, adoptive T cell therapy (ACT), including chimeric antigen receptor T cell (CAR-T) technology and T cell receptor engineered T cell (TCR-T) technology, and checkpoint blocking tumor immunotherapies (such as anti-PD- 1 and anti-PD-L1 agents), tumor vaccines and other therapeutic technologies that rely on tumor neoantigens are rapidly being developed. In this article, the relationship between neoantigens and pyroptosis and ferroptosis as well as the clinical role of neoantigens is reviewed.
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http://dx.doi.org/10.3389/fonc.2021.685377DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191503PMC
May 2021

Influence of Rapid Urbanization on Thyroid Autoimmune Disease in China.

Int J Endocrinol 2021 2;2021:9967712. Epub 2021 Jun 2.

Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: The prevalence of autoimmune thyroid diseases (AITDs), especially Hashimoto's thyroiditis (HT), has increased dramatically in China. Moreover, China is experiencing the largest scale of urbanization in the world. We intended to explore the relationship between rapid urbanization and HT.

Methods: A total of 2946 subjects in Zhejiang Shangyu (SY) ( = 1546) and Jiangsu Nanjing (NJ) ( = 1400) were enrolled in this study. Serum TPOAb, TGAb, and thyrotropin (TSH) were measured, and ultrasonography of the thyroid was performed in all subjects. DNA was extracted from all subjects, and four SNPs were selected for genotyping. Generalized multifactor dimensionality reduction (GMDR) was used to screen the best interaction between genetic factors and environment factors.

Results: TPOAb and TGAb concentrations were higher in NJ than in SY (34.60 vs. 14.00 IU/ml and 21.05 vs. 7.50 IU/ml). People in NJ also had higher TPOAb and TGAb positivity rates than those in SY (7.8% vs. 12.7% and 8.7% vs. 16.3%). Logistic regression analysis indicated that rapid urbanization was an independent risk factor for TPOAb (OR = 1.473) and TGAb (OR = 1.689). Genotype TT in rs11675434 was associated with an increased risk of TPOAb positivity both in SY (OR = 2.955) and in NJ (OR = 1.819). GMDR analysis showed a two-locus model (SNP2 × urbanization) and a three-locus model (SNP2 × SNP3 × urbanization), which had testing accuracies of 56.88% and 57.25%, respectively ( values were 0.001 and 0.001).

Conclusion: Rapid urbanization influences the incidence of TPOAb and TGAb positivity. We should pay more attention to thyroid autoimmune disease in areas of China experiencing rapid urbanization.
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http://dx.doi.org/10.1155/2021/9967712DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189768PMC
June 2021

Critical analysis of peptidoglycan structure of Lactobacillus acidophilus for phthalate removal.

Chemosphere 2021 Jun 3;282:130982. Epub 2021 Jun 3.

School of Biological Science & Biotechnology, Beijing Forestry University, 100083 Beijing, China. Electronic address:

Our previous studies have shown that lactic acid bacteria (LABs) can bind and remove di-n-butyl phthalate (DBP), diethyl phthalate, and dioctyl phthalate; three ubiquitous environmental phthalate contaminants. In this study, Lactobacillus acidophilus NCFM was chosen to study the DBP binding mechanism. We found that the three-dimensional structure of the bacterial cell wall, including the carbohydrates and proteins, was essential for DBP adsorption. Peptidoglycan was the main binding component in the cell wall (80.71%), and binding sites exposed to DBP were C-N, N-H, O-H, and C-O bonds. Molecular dynamic (MD) studies demonstrated that hydrophobic interaction plays an important role in DBP adsorption, the chemical sites that influenced the binding in the peptidoglycan model were O2, O3>N1, N2, N3>O1, O4, and the form of adsorption force included hydrogen bonding force, electrostatic force, and van der Waals forces. These theoretical data from the MD simulation were consistent with the experimental results in terms of the ability of this bacterium to bind DBP, so the MD simulation proposed a new way to investigate the mechanisms of phthalate binding to LABs.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130982DOI Listing
June 2021

Evaluation of eyestrain with vertical electrooculogram.

Comput Methods Programs Biomed 2021 May 25;208:106171. Epub 2021 May 25.

Ophthalmology Department, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.

Background And Objective: Eyestrain has been increasingly severe in our lives and works as the progress of computers and smartphones. Evaluating eyestrain helps to prevent and relieve eyestrain. Our study aimed to evaluate eyestrain by analyzing vertical electrooculogram (VEOG).

Methods: 21 young subjects were asked to watch a video on the computer for a totally 120 minutes each, during which the VEOG signal was acquired using only three electrodes, and the questionnaire was answered every 30 minutes. The VEOG signal was divided into four 30-minute phases, from which VEOG signal power probability (VEOGSPP) features and blink features were extracted. The blink features include the changes of blink number (BN), group blinks number (GBN) and ratio (GBR), mean blink amplitude (Mean_BA) and duration (Mean_BD), mean blink duration at 50% (Mean_BD50), mean closing duration (Mean_CD) and opening duration (Mean_OD), mean opening duration at early 50% (Mean_ODE50) and late 50% (Mean_ODL50), mean blink maximum rising slope (Mean_BMRS) and falling slope (Mean_BMFS).

Results: The results showed that the VEOGSPP in the high-frequency band (0.8-6.3Hz), BN, GBN, and GBR significantly increased while the VEOGSPP in the low-frequency band (0.1-0.4Hz), Mean_BA, Mean_OD, and Mean_ODL50 significantly decreased with eyestrain (P<0.05).

Conclusions: In conclusion, eyestrain induced by watching videos for a long time could be well evaluated by analyzing the VEOG signal.
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http://dx.doi.org/10.1016/j.cmpb.2021.106171DOI Listing
May 2021

Subchronic nonylphenol exposure induced anxiety-like behavior and decreased expressions of regulators of synaptic plasticity in rats.

Chemosphere 2021 Jun 1;282:130994. Epub 2021 Jun 1.

School of Public Health, Zunyi Medical University, Zunyi, Guizhou, 563000, PR China. Electronic address:

Studies have shown that there were associations between endocrine disrupting chemicals (EDCs) and anxiety. Nonylphenol (NP) is an EDC with weak estrogen activity. This study aimed to clarify whether subchronic exposure of NP at environmental concentrations induces anxiety-like behavior, and effects of NP on the regulators (NMDAR2B, PSD-95, Synapsin1) expressions of synaptic plasticity in vivo and in vitro experiments. In vivo, 40 male SD rats were randomly divided into 4 groups (each with 10 rats): low dose (0.4 mg/kg/day, L-NP), middle-dose (4 mg/kg/day, M - NP), high-dose (40 mg/kg/day, H-NP) and corn oil (Control) groups. In vitro, HT22 cells were divided into a control group (Control), NP group (NP, 20 μM), glutamine acid receptor inhibitor group (MK-801, 10 μM) and MK-801 + NP group. The concentration of NP in the hippocampus rised with the increase of NP exposure concentration in the treatment groups (F = 7.542, P = 0.001). Compared with the control group, the residence time in the dark box after NP exposure had extended (F = 117.927, P < 0.01). The duration (F = 112.054, P < 0.01) and the number of times (F = 13.514, P < 0.01) to enter the closed arm in the NP exposure group significantly increased. There were more neurons degeneration and nuclear shrinkage in the M - and H- NP groups, while the average number of shrinked neurons increased with the increasing dose of NP exposure. The protein expressions of PSD-95 (F = 97.723, P < 0.01), Synapsin1 (F = 41.797, P < 0.01) and NMDAR2B (F = 3.440, P = 0.036) in the NP group were lower than those of the control. Simultaneously, the expressions of PSD-95, Synapsin1 and NMDAR2B in the hippocampus were down-regulated; the mRNA expression of PSD-95 (F = 19.950, P < 0.01), Synapsin1 (F = 3.498, P = 0.035) and NMDAR2B (F = 9.293, P < 0.01) genes in the hippocampus decreased in the M - and H-NP groups. In vitro, the trend of the fluorescence intensity expressed by PSD-95 (F = 2.606, P = 0.124) and Synapsin1 (F = 20.573, P < 0.01) among the groups was: MK-801 + NP group < MK-801 < NP group. The protein expressions of PSD-95 (F = 5.699, P = 0.022), Synapsin1 (F = 10.820, P = 0.003) and NMDAR2B (F = 6.041, P = 0.019) were down-regulated. These results suggested that subchronic exposure to environmental concentrations of NP induced anxiety, and reduced the protein and/or mRNA expressions of regulators of synaptic plasticity (PSD-95, Synapsin1, NMDAR2B).
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http://dx.doi.org/10.1016/j.chemosphere.2021.130994DOI Listing
June 2021

Downregulation of miR-892b inhibits the progression of osteoarthritis via targeting cyclin D1 and cyclin D2.

Exp Cell Res 2021 Jun 5;405(2):112683. Epub 2021 Jun 5.

Department of Orthopedic Surgery, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan, 450003, China. Electronic address:

Backgrounds: Osteoarthritis (OA) is an orthopedic inflammatory disease which can cause functional disability and chronic pain. MiRNAs are known to play important roles in OA. To identify the targets for the treatment of OA, bioinformatics analysis was performed to explore differentially expressed miRNAs between OA and normal samples.

Methods: Bioinformatics analysis was conducted to identify differentially expressed miRNAs. To mimic OA in vitro, primary chondrocytes were stimulated with IL-1β. Meanwhile, flow cytometry was performed to detect the cell apoptosis and cycle distribution. In addition, protein and mRNA expressions were detected by Western blot and RT-qPCR, respectively. Finally, in vivo model of OA was constructed to investigate the function of miR-892b in OA.

Results: The data indicated that miR-892b was identified to be upregulated in OA samples. Additionally, miR-892b antagomir markedly reversed IL-1β-induced growth decline of chondrocytes via inhibiting the apoptosis. IL-1β notably elevated the expressions of MMP1 and MMP13 and downregulated the level of Aggrecan in chondrocytes, while miR-892b antagomir reversed these phenomena. Meanwhile, cyclin D1 and cyclin D2 were the direct targets of miR-892b. In addition, IL-1β-induced G1 phase arrest in chondrocytes was partially abolished by of miR-892b antagomir. In vivo study indicated miR-892b antagomir could significantly alleviate the symptom of OA in a rat model.

Conclusion: MiR-892b antagomir inhibits the progression of OA via targeting Cyclin D1 and Cyclin D2. Thus, our finding might supply a novel target for OA treatment.
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http://dx.doi.org/10.1016/j.yexcr.2021.112683DOI Listing
June 2021

1,25-Dihydroxyvitamin D inhibits porcine epidemic diarrhea virus replication by regulating cell cycle resumption in IPEC-J2 porcine epithelial cells.

Microb Pathog 2021 Jun 7;158:105017. Epub 2021 Jun 7.

Institute of Animal Nutrition, Sichuan Agricultural University, NO. 46 Xinkang Road, Yucheng District, Yaan, Sichuan, 625014, People's Republic of China. Electronic address:

Porcine epidemic diarrhea virus (PEDV) infection causes heavy economic losses in the pig industry. Currently, the lack of effective treatments prompts new antiviral researches. We have shown that 25-hydroxyvitamin D supplementation alleviated PEDV infection in weaned pigs before. However, it is not clear whether vitamin D inhibits PEDV replication. In this study, 1,25-dihydroxyvitamin D (1,25(OH)D) inhibited PEDV induced mitochondria damage and cell apoptosis. In addition, 1,25(OH)D treatment decreased PEDV nucleocapsid gene and protein levels in IPEC-J2 cells. Transcriptomic data showed that PEDV infection altered the expression of 5316 genes (2498 up, 2818 down) in IPEC-J2 cells. The differentially expressed genes were mainly involved in cell cycle process, ribonucleoprotein complex biogenesis, mitotic nuclear division, and other biological processes. Then we examined the effects of PEDV infection on cell cycle progression in IPEC-J2 cells, and the results showed that PEDV induced G0/G1 phase arrest. G0/G1-phase arrest was also conducive to PEDV replication. However, 1,25(OH)D treatment decreased G0/G1 phase percentage induced by PEDV. Cyclin D and cyclin E mRNA expression were also increased by 1,25(OH)D supplementation upon PEDV infection. Moreover, the regulation of 1,25(OH)D on cell cycle progression was abrogated by ERK1/2 inhibitor, as well as the mRNA expression of cyclin D. The inhibition of 1,25(OH)D on PEDV replication was also eliminated by ERK1/2 inhibitor. Taken together, these results demonstrated that 1,25(OH)D supplementation inhibited PEDV replication, and the anti-virus effect of 1,25(OH)D was mediated in part by regulating cell cycle progression through ERK1/2 signaling pathway.
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http://dx.doi.org/10.1016/j.micpath.2021.105017DOI Listing
June 2021

Fermented Diet Liquid Feeding Improves Growth Performance and Intestinal Function of Pigs.

Animals (Basel) 2021 May 19;11(5). Epub 2021 May 19.

Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China.

Accumulating evidences demonstrate that fermented feed and liquid feeding exerted a great beneficial influence on growth performance and health in the pig industry. This experiment was conducted to evaluate the effects of fermented liquid feeding on the growth performance and intestinal function of pigs. Two hundred and eighty-eight 27-day-old weaned piglets (8.21 ± 0.27 kg) were randomly allocated to a control group (basal diet (CON)), an antibiotic group (basal diet supplemented with antibiotics (AB)) and a fermented liquid feeding group (basal diet with fermented liquid feeding (FLF)), with 6 replicates per treatment and 16 weaned piglets per replicate. The experiment lasted for 160 days. Fresh fecal samples were collected to evaluate the apparent total tract digestibility (ATTD) of nutrients from the last 4 days of each stage. The results are shown as follows: (1) Compared with the CON group, in the whole stage, the FLF diet significantly increased the final body weight (BW) and ADG of pigs ( < 0.05), and had a tendency to increase ADFI ( = 0.086), but had no effect on F/G. (2) The ATTD of dry matter (DM), crude protein (CP), ether extract (EE), crude ash (CA), crude fiber (CF), gross energy (GE), calcium (Ca) and total phosphorus (TP) in the FLF group was significantly elevated compared with those of the CON group at 8-20 kg stage ( < 0.05). Meanwhile, the ATTD of EE in the FLF group was significantly increased compared with that of the CON group at the 50-75 kg and 100-125 kg stages ( < 0.05), and the ATTD of Ca was higher than that of CON group at the 100-125 kg stage ( < 0.05). (3) Compared with that of the CON group, the level of serum leptin in the FLF group had a tendency to decrease ( = 0.054), the level of serum ghrelin in the FLF group was significantly elevated ( < 0.05) and the level of serum peptide YY in the FLF group was significantly decreased ( < 0.05). (4) The abundance of in cecal and colonic digesta was observably enhanced in FLF group. Meanwhile, the abundance of in cecal and colonic digesta were dramatically reduced in the FLF group compared with that in the CON and AB groups ( < 0.05). (5) The levels of acetic acid in colonic digesta were significantly increased in the FLF group ( < 0.05), and an increasing trend was observed in total VFA in colonic digesta compared with CON ( < 0.1). The levels of acetic acid in colonic digesta were significantly promoted in the FLF group compared with that of the AB group ( < 0.05). In conclusion, these results indicate that fermented liquid feeding improved the growth performance of pigs, which might be associated with gastrointestinal hormone and intestinal functions.
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http://dx.doi.org/10.3390/ani11051452DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158733PMC
May 2021

Procyanidin B2 induces porcine skeletal slow-twitch myofiber gene expression by AMP-activated protein kinase signaling pathway.

Anim Biotechnol 2021 Jun 1:1-10. Epub 2021 Jun 1.

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, P. R. China.

In this study, our aim is to investigate the effect of dimer procyanidin B2 [epicatechin-(4β-8)-epicatechin] (PB2) on porcine skeletal myofiber gene expression . Our data showed PB2 promoted the protein expression of slow myosin heavy chain (MyHC) in porcine myotubes, concomitant with the increases in mRNA levels of , and . We also found PB2 activated AMPK signaling in porcine myotubes. NRF1 and CaMKKβ that are two important upstream factors of AMPK, and Sirt1 and PGC-1α that are two major downstream factors of AMPK, were also up-regulated by PB2. The mechanism study showed the effect of PB2 on slow-twitch myofiber gene expression was abolished by AMPK inhibitor compound C or by AMPKα1 siRNA. Together, we found PB2 induced porcine skeletal slow-twitch myofiber gene expression by AMPK signaling pathway.
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http://dx.doi.org/10.1080/10495398.2021.1927065DOI Listing
June 2021

Selective dispersion of neutral nanoplates and the interfacial structure of copolymers based on coarse-grained molecular dynamics simulations.

Soft Matter 2021 May 28. Epub 2021 May 28.

College of Materials and Metallurgy, Guizhou University, Guiyang 550025, China. and National Engineering Research Center for Compounding and Modification of Polymer Materials, Guiyang 550058, China.

The selective dispersion of neutral nanoplates (NNP) and the control of the interfacial structure of copolymers are challenging. In this work, we employ coarse-grained molecular dynamics (CGMD) to investigate the dispersion of NNP and the interfacial structure. The introduction of NNP significantly changes the interfacial structure and formation mechanism of diblock copolymers (DBCP), which is related to the matrix phase, distribution, composition, and length of two different chain segments (A and B) in AmBn-DBCP. The phase-weak groups that have a poor interaction with NNP will stack easily, whereas the stacking degree for the phase-rich groups that have a strong interaction with NNP decreases due to the addition of NNP. The interaction between two phases will be enhanced, which is favorable for the formation of a random network structure. Due to the strong interaction of the phase-rich groups with NNP, the NNP change the accumulation types of phase-weak groups and enhances the combination of two chain segments in favor of the formation of a cylindrical micelle-like structure. The transmission electron microscopy (TEM) images show that layered double hydroxide (LDH) orientationally distributes in the acrylic acid chain segments in ethylene acrylic acid (EAA) random copolymers, which is in agreement with the theoretical simulation results. This proves that the selective dispersion of LDH in copolymers affects their interfacial structure.
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http://dx.doi.org/10.1039/d1sm00352fDOI Listing
May 2021

Percutaneous Microwave Ablation Versus Open Surgical Resection for Colorectal Cancer Liver Metastasis.

Front Oncol 2021 11;11:638165. Epub 2021 May 11.

People's Liberation Army General Hospital, Beijing, China.

Purpose: To compare the therapeutic outcomes between open surgical resection (OSR) and percutaneous microwave ablation (PMWA) for colorectal liver metastasis (CRLM) ≤3 cm.

Methods: In this retrospective study, 200 consecutive patients with 306 CRLMs were reviewed. Overall survival (OS), disease-free survival (DFS), local tumour progression (LTP), intrahepatic distant recurrence, and extrahepatic metastasis were analysed to compare the therapeutic efficacy. Cox proportional hazards regression analysis was used to identify the prognostic factors for OS and DFS. Major complications and postoperative hospital stay were also assessed.

Result: The 1-, 3-, and 5-year OS rates were 91.6%, 64.1%, and 46.3%, respectively, in the PMWA group and 89.7%, 62.4% and 44.7%, respectively, in the OSR group (P=0.839). The 1-, 3-, and 5-year DFS rates were 61.9%, 44.8%, and 41.3%, respectively, in the PMWA group and 58.1%, 24.4%, and 18.3%, respectively, in the OSR group (P =0.066). The two groups had comparable 5-year cumulative rates of intrahepatic distant recurrence (P=0.627) and extrahepatic metastasis (P=0.884). The 5-year cumulative LTP rate was lower in the OSR group than in the PMWA group (P=0.023). The rate of major complications was higher in the OSR group than in the PMWA group (P =0.025), and the length of hospital stay after treatment was shorter in the PMWA group (P<0.001).

Conclusion: There were no significant differences in OS or DFS between the two groups. PMWA was associated with increased LTP, fewer postoperative days and fewer major complications.
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http://dx.doi.org/10.3389/fonc.2021.638165DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144705PMC
May 2021

Hospital-based case control study and animal study on the relationship between nonylphenol exposure and depression.

PeerJ 2021 18;9:e11384. Epub 2021 May 18.

School of Public Health, Zunyi Medical University, Zunyi, Guizhou, PR China.

Objectives: The aim of this work is to explore the association between chronic exposure to nonylphenol (NP), a representative environmental endocrine disruptor (EED), and the risk of depression and its potential mechanism.

Methods: A hospital-based case control study was conducted from August to December 2018. Forty-one patients with confirmed depression and 47 healthy volunteers were recruited. In vitro, 20 rats were randomly divided into the control group (corn oil) and NP exposure group (=10 per group), which were gavaged at a dose of 4 mg/kg w/day for 180 days.

Results: The depressed patient group had higher Zung Self-Rating Depression Scale (SDS) (<0.001) and Self-Rating Anxiety Scale (SAS) (<0.001) scores than the healthy group. The serum NP level (=0.009) in the depressed group was higher than that in the healthy group, and the BDNF level (=0.001) was lower. The serum levels of monoamine neurotransmitters dopamine (DA) (=0.070), epinephrine (E) (=0.001), and noradrenaline (NE) (=0.000) were lower than those in the healthy group. In the sucrose preference test, the sucrose preference time for the exposure group of rats was lower than that of the control group (<0.001). In the forced swim test, a longer resting time was measured for the exposure group of rats as compared to the control group (<0.05). The level of NP (<0.001) in the brain tissue of the NP exposure group was higher than that in the control group, and the serum level of brain-derived neurotrophic factor (BDNF) (=0.004) was lower. Histopathological examination of the brain biopsies illustrated that the neuronal cells and nuclei in the hippocampus of the exposed group exhibited slight shrinkage.

Conclusion: Chronic exposure to NP at environmental doses will result in the accumulation of NP in the brain and blood, and induction of depression, which might be associated with the alterations in the expression levels of BDNF and monoamine neurotransmitters.
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http://dx.doi.org/10.7717/peerj.11384DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139269PMC
May 2021

Symptomatic aseptic necrosis of benign thyroid lesions after microwave ablation: risk factors and clinical significance.

Int J Hyperthermia 2021 ;38(1):815-822

Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing, China.

Background: Symptomatic aseptic necrosis (SAN) followed by nodule rupture is a kind of severe complications after thermal ablation for benign thyroid nodules (BTN). No studies are available to evaluate its pathologic process, clinical manifestations, risk factors and effectiveness of therapies after microwave ablation (MWA).

Methods: From 2012 to 2019, 398 patients who received MWA for BTN were retrospectively reviewed. Clinical data included baseline patient characteristics, imaging features (internal vascularity and the proportion of the solid component), ablation power and time, complications and prognosis were collected and documented.

Results: Ten patients (2.51%) experienced post-MWA SAN, eight patients with nodule rupture and the other two without. The mean time from MWA to SAN symptom was 8.6 days and to rupture was 16.3 days. The initial symptoms of SAN patients were neck bulging, swelling and discomfort. Patients would go through nodule rupture once the nodule contents extended into the extrathyroidal area with the discontinuity of the anterior thyroid capsule, and fistula formed unavoidably in this condition. Incision drainage was effective for rupture and early treatment of non-steroidal anti-inflammatory drug might cure the early-stage SAN. Multivariate analysis showed sex (OR = 0.13; 95% CI: 0.03, 0.61; =.03) was the risk factor leading to SAN and males were more vulnerable to SAN.

Conclusion: SAN after MWA came earlier and initially illustrated as neck bulging, swelling and discomfort. Early detection and early treatment might prevent the rupture of nodules. Once the breakdown of thyroid capsule occurred, rupture of ablated nodules out of skin was unavoidable and invasive procedures might be the most effective treatment.
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http://dx.doi.org/10.1080/02656736.2021.1930203DOI Listing
January 2021

3DCANN: A Spatio-Temporal Convolution Attention Neural Network for EEG Emotion Recognition.

IEEE J Biomed Health Inform 2021 May 25;PP. Epub 2021 May 25.

Since electroencephalogram (EEG) signals can truly reflect human emotional state, emotion recognition based on EEG has turned into a critical branch in the field of artificial intelligence. Aiming at the disparity of EEG signals in various emotional states, we propose a new deep learning model named three-dimension convolution attention neural network (3DCANN) for EEG emotion recognition in this paper. The 3DCANN model is composed of spatio-temporal feature extraction module and EEG channel attention weight learning module, which can extract the dynamic relation well among multi-channel EEG signals and the internal spatial relation of multi-channel EEG signals during continuous time period. In this model, the spatio-temporal features are fused with the weights of dual attention learning, and the fused features are input into softmax classifier for emotion classification. In addition, we utilize SJTU Emotion EEG Dataset (SEED) to appraise the feasibility and effectiveness of the proposed algorithm. Finally, experimental results display that the 3DCANN method has superior performance over the state-of-the-art models in EEG emotion recognition.
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http://dx.doi.org/10.1109/JBHI.2021.3083525DOI Listing
May 2021

A CDC7 inhibitor sensitizes DNA-damaging chemotherapies by suppressing homologous recombination repair to delay DNA damage recovery.

Sci Adv 2021 May 21;7(21). Epub 2021 May 21.

Oncology Drug Discovery Unit, Takeda Pharmaceutical Company Limited, Fujisawa, Japan.

Cell division cycle 7 (CDC7), a serine/threonine kinase, plays important roles in DNA replication. We developed a highly specific CDC7 inhibitor, TAK-931, as a clinical cancer therapeutic agent. This study aimed to identify the potential combination partners of TAK-931 for guiding its clinical development strategies. Unbiased high-throughput chemical screening revealed that the highest synergistic antiproliferative effects observed were the combinations of DNA-damaging agents with TAK-931. Functional phosphoproteomic analysis demonstrated that TAK-931 suppressed homologous recombination repair activity, delayed recovery from double-strand breaks, and led to accumulation of DNA damages in the combination. Whole-genome small interfering RNA library screening identified sensitivity-modulating molecules, which propose the experimentally predicted target cancer types for the combination, including pancreatic, esophageal, ovarian, and breast cancers. The efficacy of combination therapy in these cancer types was preclinically confirmed in the corresponding primary-derived xenograft models. Thus, our findings would be helpful to guide the future clinical strategies for TAK-931.
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http://dx.doi.org/10.1126/sciadv.abf0197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139593PMC
May 2021

Telomere dynamics across the early life course: Findings from a longitudinal study in children.

Psychoneuroendocrinology 2021 Jul 14;129:105270. Epub 2021 May 14.

Columbia Center for Children's Environmental Health, Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, NY 10032, USA; Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, NY 10032, USA.

Telomeres are protective caps on chromosome ends that shorten with each cell division. Telomere length (TL) predicts the onset of cellular senescence and correlates with longevity and age-related disease risk. Previous research suggests that adults display fixed ranking and tracking of TL by age 20 years, supporting the importance of TL at birth and attrition during childhood. However, longitudinal research examining telomere dynamics during early life is sparse. Here, we used monochrome multiplex quantitative polymerase chain reaction to measure relative TL in leukocytes isolated from cord blood and child blood collected at ages 3, 5, 7, and 9 years among 224 minority children enrolled in a New York City-based birth cohort. We also measured maternal TL at delivery in a subset of 197 participants with a biobanked blood sample. TL decreased most rapidly in the first years of life (birth to 3 years), followed by a period of maintenance into the pre-puberty period. Mothers with longer telomeres gave birth to newborns with longer telomeres that remained longer across childhood, suggesting that the fixed ranking and tracking of TL observed among adults may extend to early childhood or even the prenatal period with a potential transgenerational basis. We did not find significant sex differences in the pattern of child TL change across development. These findings emphasize the need to understand factors and mechanisms that determine TL during early childhood.
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http://dx.doi.org/10.1016/j.psyneuen.2021.105270DOI Listing
July 2021

Cytogenetic and mutational analysis and outcome assessment of a cohort of 284 children with de novo acute myeloid leukemia reveal complex karyotype as an adverse risk factor for inferior survival.

Mol Cytogenet 2021 May 19;14(1):27. Epub 2021 May 19.

Department of Pathology, School of Medicine, University of Colorado Anschutz Medical Campus, 12705 E. Montview Boulevard, Suite 400, Aurora, CO, 80045, USA.

Background: Acute myeloid leukemia (AML) is rare in children. Although complex karyotype (CK) defined as ≥ 3 cytogenetic abnormalities is an adverse risk factor in adult AML, its prognostic impact on childhood AML remains to be determined.

Results: We studied the prevalence, cytogenetic and mutational features, and outcome impact of CK in a cohort of 284 Chinese children with de novo AML. Thirty-four (12.0%) children met the criteria for CK-AML with atypical CK being more frequent than typical CK featured with -5/5q-, -7/7q-, and/or 17p aberration. Mutational prevalence was low and co-occurrence mutants were uncommon. Children with CK-AML showed shorter overall survival (OS) (5-year OS: 26.7 ± 10.6% vs. 37.5 ± 8.6%, p = 0.053) and event-free survival (EFS) (5-year EFS: 26.7 ± 10.6% vs. 38.8 ± 8.6%, p = 0.039) compared with those with intermediate-risk genetics. Typical CK tended to correlate with a decreased OS than atypical CK (5-year OS: 0 vs. 33 ± 12.7%.; p = 0.084), and CK with ≥ 5 cytogenetic aberrations was associated with an inferior survival compared with CK with ≤ 4 aberrations (5-year OS: 13.6 ± 11.7% vs. 50.0 ± 18.6%; p = 0.040; 5-year EFS: 13.6 ± 11.7% vs. 50.0 ± 18.6%; p = 0.048).

Conclusion: Our results demonstrate CK as an adverse risk factor for reduced survival in childhood AML. Our findings shed light on the cytogenetic and mutational profile of childhood CK-AML and would inform refinement of risk stratification in childhood AML to improve outcomes.
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http://dx.doi.org/10.1186/s13039-021-00547-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136172PMC
May 2021

Infusion of short chain fatty acids in the ileum improves the carcass traits, meat quality and lipid metabolism of growing pigs.

Anim Nutr 2021 Mar 22;7(1):94-100. Epub 2020 Oct 22.

Institute of Animal Nutrition, Sichuan Agricultural University, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Key Laboratory of Animal Disease-resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Disease-resistant Nutrition of Sichuan Province, Ya'an, 625014, China.

Short chain fatty acids (SCFA) are the main products of indigestible carbohydrates undergoing bacterial fermentation in the hindgut, which are related to some physiological functions. This study was designed to investigate the effects of SCFA infusion by ileum on the carcass traits, meat quality and lipid metabolism of growing pigs. In a 28-day study, 24 growing barrows fitted with a T-cannula in distal ileum were divided into 4 treatments: 1) Control, 2) antibiotics (AB), 3) AB + 300 mL of SCFA1 solution (ABS1), 4) AB + 300 mL of SCFA2 solution (ABS2). The concentrations of acetate, propionate and butyrate in SCFA1 solution were respectively 61.84, 18.62 and 12.55 mmol/L, and in SCFA2 were respectively 40.08, 15.41 and 9.78 mmol/L. The results showed that the SCFA infusion increased the average daily feed intake and average daily gain of pigs ( 0.05). Meanwhile, the SCFA treatments increased longissimus dorsi area ( 0.05) and carcass weight ( = 0.058), decreased the drip loss of longissimus dorsi ( = 0.059), and reduced serum concentrations of triglyceride, total cholesterol and urea nitrogen ( 0.05). Besides, the SCFA administration inhibited the mRNA expressions of fatty acid synthase () and acetyl-CoA carboxylase in longissimus dorsi ( 0.05), the mRNA expression of in the liver ( 0.05), and the mRNA expression of hormone-sensitive lipase in abdominal fat ( 0.05). Short chain fatty acid infusion also enhanced the mRNA expression of carnitine palmitoyltransferase-1α in the liver ( 0.05), the mRNA expressions of peroxisome proliferator activated receptor gamma and lipoprotein lipase in abdominal fat ( 0.05), and the mRNA expressions of free fatty acid receptor 2, glucose-6-phosphatase and phosphoenolpyruvate carboxykinase 1 in the colon ( 0.05). These results suggested that SCFA administration in the ileum could improve the carcass traits and meat quality of growing pigs, which was possibly due to the fact that SCFA modulated lipid metabolism.
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http://dx.doi.org/10.1016/j.aninu.2020.05.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110845PMC
March 2021

Metal-Free Direct C-H Functionalization of Quinoxalin-2(1)-Ones to Produce 3-Vinylated Quinoxalin-2(1)-Ones in the Presence of Alkenes.

Front Chem 2021 30;9:672051. Epub 2021 Apr 30.

School of Pharmacy, Weifang Medical University, Weifang, China.

A novel and efficient - vinylation reaction with quinoxalin-2(1)-one as the substrate, in the presence of alkenes, under metal-free conditions, is reported herein. The reaction leads to the formation of new carbon-carbon bonds that exhibit moderate to good reactivities. The vinylation of quinoxalin-2(1)-ones, in the presence of alkenes, is an attractive process that can be potentially utilized to produce biologically active 3-vinylated quinoxalin-2(1)-ones.
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http://dx.doi.org/10.3389/fchem.2021.672051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119786PMC
April 2021

L-Proline: An Effective Agent for Frozen and Post-thawed Donkey Semen Storage.

J Equine Vet Sci 2021 Jun 3;101:103393. Epub 2021 Feb 3.

National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China. Electronic address:

The aim of this study was to evaluate the effects of L-proline on the extender quality of frozen and post-thawed jackass semen. Jackass (n = 6) semen samples were collected and cryopreserved in gradient concentrations (0-80 mM) of L-proline in extenders; post-thawed semen samples were cultured in L-proline medium for 10 hours at 37°C. For cryopreservation experiment I, the motile parameters, mitochondrial membrane potential (MMP), and plasma membrane, acrosome, and chromatin structure integrities of post-thawed semen were assessed. For culture experiment II, additional ROS contents were analyzed after incubation. For the fertility trial, jennies (n = 135) were divided into group I (30 mM L-proline in cryopreservation extender), group II (40 mM L-proline in culture medium), and the control. Pregnancy was diagnosed using an ultrasound scanner 30 days after ovulation. The results of experiment I showed that, motile parameters and acrosome and chromatin structure integrities of groups I and 40 mM were significantly higher than the control (P < .05). MMP of group I was significantly higher than the control and 40 mM groups (P < .05). In experiment II, after 4 hours of incubation, motile parameters, MMP, and DNA integrity in group II were significantly higher than the control (P < .05). Additionally, 40 and 80 mM L-proline in culture medium significantly reduced ROS accumulation after 4 and 10 hours of incubation (P < .05). Pregnancy rates of the control and groups I and II were 28.85%, 40%, and 36.84%, respectively. In conclusion, the extenders containing 30 to 40 mM L-proline improved both qualities of frozen and post-thawed semen, and it will be a beneficial agent for donkey frozen spermatozoa or post-thawed semen storage.
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http://dx.doi.org/10.1016/j.jevs.2021.103393DOI Listing
June 2021