Publications by authors named "Jie Yang"

3,550 Publications

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Metal-Organic Gel Derived N-Doped Granular Carbon: Remarkable Toluene Uptake and Rapid Regeneration.

ACS Appl Mater Interfaces 2021 Apr 12. Epub 2021 Apr 12.

State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, P. R. China.

Porous carbon materials with chemical and thermal stability and high porosity have been widely used for volatile organic compound (VOC) purification. Designing granular carbon with remarkable adsorption capacity and rapid regeneration is of great significance for the capture of VOCs from high humidity air. Herein, a series of N-doped granular carbons were synthesized by direct carbonization of metal-organic gel (MOG). The N-doped granular carbons (C700 and C700K) feature high surface area, hierarchical pore, and abundant N,O multifunctional groups. The toluene adsorption capacity of C700K is highly improved (9.0 mmol/g toluene at / = 0.1) in comparison with MOG (4.81 mmol/g toluene at / = 0.1). The toluene breakthrough time of C700K is over 4 times longer than that of MOG at wet conditions (60% RH, 298 K), also much longer than that of widely used carbon materials, zeolites, and representative MOFs, including BPL activated carbon, coconut shell activated carbon, carbosieve, ZSM-5, and MIL-101(Cr). Furthermore, the N-doped granular carbons also exhibit excellent hydrophobicity and can be regenerated rapidly. The internal pore channel and desorption kinetics reveal that the effective diffusion length plays a critical role in the regeneration rate.
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http://dx.doi.org/10.1021/acsami.1c01524DOI Listing
April 2021

Nutritional status and risk of contrast-associated acute kidney injury in elderly patients undergoing percutaneous coronary intervention.

Clin Exp Nephrol 2021 Apr 12. Epub 2021 Apr 12.

Department of Geriatric Medicine, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fujian Provincial Institute of Clinical Geriatrics, Fujian Key Laboratory of Geriatrics, Fujian Provincial Center for Geriatrics, Fuzhou, 350001, Fujian, China.

Background: This study aimed to investigate the connection between malnutrition evaluated by the Controlling Nutritional Status (CONUT) score and the risk of contrast-associated acute kidney injury (CA-AKI) in elderly patients who underwent percutaneous coronary intervention (PCI).

Methods: A total of 1308 patients aged over 75 years undergoing PCI was included. Based on the CONUT score, patients were assigned to normal (0-1), mild malnutrition (2-4), moderate-severe malnutrition group (≥ 5). The primary outcome was CA-AKI (an absolute increase in ≥ 0.3 mg/dL or ≥ 50% relative serum creatinine increase 48 h after contrast medium exposure).

Results: Overall, the incidence of CA-AKI in normal, mild, moderate-severe malnutrition group was 10.8%, 11.0%, and 27.2%, respectively (p < 0.01). Compared with moderate-severe malnutrition group, the normal group and the mild malnutrition group showed significant lower risk of CA-AKI in models adjusting for risk factors for CA-AKI and variables in univariate analysis (odds ratio [OR] = 0.48, 95% confidence interval [CI]: 0.26-0.89, p = 0.02; OR = 0.46, 95%CI: 0.26-0.82, p = 0.009, respectively). Furthermore, the relationship were consistent across the subgroups classified by risk factors for CA-AKI except anemia. The risk of CA-AKI related with CONUT score was stronger in patients with anemia. (overall interaction p by CONUT score = 0.012).

Conclusion: Moderate-severe malnutrition is associated with higher risk of CA-AKI in elderly patients undergoing PCI.
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http://dx.doi.org/10.1007/s10157-021-02061-4DOI Listing
April 2021

Visible-Light-Induced Intermolecular Oxyimination of Alkenes.

Org Lett 2021 Apr 12. Epub 2021 Apr 12.

Gansu International Scientific and Technological Cooperation Base of Water-Retention Chemical Functional Materials; Key Laboratory of Eco-Environment-Related Polymer Materials Ministry of Education; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, Gansu 730070, China.

An intermolecular vicinal O-N difunctionalization reaction of olefins with oxime esters through energy transfer catalysis has been developed.
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http://dx.doi.org/10.1021/acs.orglett.1c01064DOI Listing
April 2021

Gastric metastasis from pancreatic cancer characterized by mucosal erosion: a case report and literature review.

J Int Med Res 2021 Apr;49(4):3000605211003759

Department of Gastroenterology, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, Jiangsu, China.

Pancreatic cancer with gastrointestinal tract metastasis is a fairly rare occurrence, and gastric metastasis in such cases has been seldom reported. We herein present a case of gastric involvement secondary to pancreatic cancer in a 74-year-old woman in whom the metastatic lesion only presented as mucosal erosion in the stomach. The patient had a 1-month history of progressive right upper quadrant pain before admission. Computed tomography and endoscopic examinations revealed a solid and hypo-enhancing mass in the head of the pancreas. The patient underwent conventional upper endoscopy before pancreatic biopsy, and mucosal erosion was observed in the gastric pylorus. We obtained gastric and pancreatic biopsies by gastroscopy and endoscopic ultrasound-guided fine needle aspiration, respectively. Pathologically, the biopsies taken from the area of gastric erosion showed poorly differentiated invasive adenocarcinoma that was morphologically consistent with the pancreatic specimens. Moreover, the gastric section showed tumor thrombi within the vessels. Hence, the suspected diagnosis was unresectable pancreatic cancer with gastric metastasis. The patient immediately underwent two courses of chemotherapy, but her condition rapidly deteriorated and she died 2 months later.
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http://dx.doi.org/10.1177/03000605211003759DOI Listing
April 2021

Association between pyrethroid exposure and cardiovascular disease: A national population-based cross-sectional study in the US.

Environ Int 2021 Apr 9;153:106545. Epub 2021 Apr 9.

Department of Epidemiology and Biostatistics, Ministry of Education & Ministry of Environmental Protection Key Laboratory of Environment and Health, and State Key Laboratory of Environmental Health (incubation), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China; The George Institute for Global Health, University of New South Wales, Sydney, New South Wales, Australia; Division of Epidemiology, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA. Electronic address:

Objective: Pyrethroids-containing products are widely used as commercial and household insecticides. While animal studies and clinical case reports have shown acute cardiovascular outcomes of pyrethroids exposure, little has been known on the effect of chronic pyrethroid exposure on cardiovascular disease (CVD). We aimed to examine the associations between chronic pyrethroid exposure and CVD in the US adults.

Methods: Cross-sectional data from the National Health and Nutrition Examination Survey 1999-2002 and 2007-2012 were analyzed. The exposure to pyrethroids was determined as the urinary level of 3-phenoxybenzoic acid (3-PBA), and CVD was ascertained based on self-reported physician diagnoses. Multivariable logistic regression models were fitted to evaluate associations of pyrethroid exposure with CVD, coronary heart disease (CHD), and stroke.

Results: Included were 6,471 participants with a mean age of 44.77 years (standard error, 0.39) for final analyses. The weighted prevalence of CVD, CHD, and stroke was 6.85%, 4.57% and 2.27%, respectively. With adjustments for major confounders, participants in the highest tertile of urinary 3-PBA had higher odds of CVD (odds ratio, 1.58; 95% confidence interval: 1.12, 2.23) and CHD (OR, 1.75; 95% CI: 1.17, 2.61) compared to those in the lowest tertile. There were linear associations for CVD (P for trend = 0.04) and CHD (P for trend = 0.02). However, no significant association was noted for stroke (1.29; 0.78, 2.16) in the main analyses.

Conclusions: 3-PBA was adversely associated with CVD and CHD in the US adults. Our findings highlight potential cardiovascular risk of chronic exposure to pyrethroids, and should be validated in large prospective studies in different populations in future.
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http://dx.doi.org/10.1016/j.envint.2021.106545DOI Listing
April 2021

Preparation and stability mechanisms of double emulsions stabilized by gelatinized native starch.

Carbohydr Polym 2021 Jun 16;262:117926. Epub 2021 Mar 16.

Key Laboratory of Synergetic and Biological Colloids, Ministry of Education, Wuxi, 214122, Jiangsu Province, China; Qingdao Special Food Research Institute, Qingdao, 266109, Shandong Province, China; School of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu Province, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, 214122, Jiangsu Province, China. Electronic address:

Double emulsions are promising carrier systems for foods, pharmaceuticals, and cosmetics. However, their limited stability hinders their practical applications. We used gelatinized starch to develop stable double emulsions as carrier materials. The oil/water/water (O/W/W) double emulsions were formed by 5 wt% native corn starch, while oil/water/oil (O/W/O) double emulsions were formed by 7 wt% native corn starch and high-amylose starch with 60 % and 75 % amylose contents investigated by optical microscopy. Furthermore, the storage stability of double emulsions was revealed by droplet size distribution, microstructure, backscattering, rheological profiles, and low-field nuclear magnetic resonance (LF-NMR) imaging. Results confirmed that the O/W/O double emulsions stabilized by 7 wt% native corn starch had a smaller mean droplet size (11.400 ± 0.424 μm) and excellent storage stability (14 days) than O/W/W and O/W/O double emulsions prepared with high-amylose starch. Such unique double emulsions prepared with gelatinized native corn starch are good candidates of carrier materials.
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http://dx.doi.org/10.1016/j.carbpol.2021.117926DOI Listing
June 2021

GILT in tumor cells improves T cell-mediated anti-tumor immune surveillance.

Immunol Lett 2021 Apr 7. Epub 2021 Apr 7.

Department of Biochemistry and Molecular Biology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China; Key Laboratory of Immune Microenvironment and Disease, Ministry of Education, Key Laboratory of Cellular and Molecular Immunology in Tianjin, Excellent Talent Project, Tianjin Medical University, Tianjin, China. Electronic address:

The lysosomal thiol reductase GILT catalyzes the reduction of disulfide bonds of protein antigens, facilitating antigen-presenting cells (APCs) to present antigen to T cells. However, whether GILT expression in tumor cells can be associated with improved T cell-mediated anti-tumor responses remains unknown. Here, we identify that GILT is able to facilitate anti-tumor immune surveillance via promoting MHC class I mediated-antigen presentation in colon carcinoma. By using mice model bearing colon tumors, we find that GILT inhibites tumor growth in vivo with more leucocytes infiltration but has no effect on tumor cell development in vitro in terms of proliferation, cell cycle and migration. Furthermore, by using transgenic OT-I mice, we recognize the tumor-expressing OVA peptide, a surrogate tumor antigen, we find that GILT is capable of enhancing MHC class I mediated antigen presentation and improving specific CD8 T cell anti-tumor responses in murine colon carcinoma. These findings propose the boost of GILT-MHC-I axis in tumors as a viable option for immune system against cancer.
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http://dx.doi.org/10.1016/j.imlet.2021.04.001DOI Listing
April 2021

CCR8 marks highly suppressive Treg cells within tumours but is dispensable for their accumulation and suppressive function.

Immunology 2021 Apr 10. Epub 2021 Apr 10.

Department of Pathology, University of Cambridge, Tennis Court Road, CB2 1QP, Cambridge, UK.

CD4 regulatory T (Treg) cells, dependent upon the transcription factor Foxp3, contribute to tumour immunosuppression but are also required for immune homeostasis. There is interest in developing therapies that selectively target the immunosuppressive function of Treg cells within tumours without disrupting their systemic anti-inflammatory function. High levels of expression of Chemokine (C-C motif) receptor 8 (CCR8) discriminate Treg cells within tumours from those found in systemic lymphoid tissues. It has recently been proposed that disruption of CCR8 function using blocking anti-CCR8 antibodies results in reduced accumulation of Treg cells within tumours and disruption of their immunosuppressive function. Here, using Ccr8 mice, we show that CCR8 function is not required for Treg cell accumulation or immunosuppression in the context of syngeneic MC38 colorectal adenocarcinoma and B16 melanoma tumours. We observed high levels of CCR8 expression on tumour-infiltrating Treg cells which was abolished in Ccr8 mice. High levels of CCR8 marked cells with high levels of suppressive function. However, whereas systemic ablation of Treg cells resulted in strikingly diminished tumour burden, growth of subcutaneously implanted tumours was unaffected by systemic CCR8 loss. Consistently, we observed minimal impact of systemic CCR8 ablation on the frequency, phenotype and function of tumour-infiltrating Treg cells and conventional T (Tconv) function. These findings suggest that CCR8 is not required for Treg cell accumulation and immunosuppressive function within tumours and that depletion of CCR8 Treg cells rather than blockade of CCR8 function is a more promising avenue for selective immunotherapy.
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http://dx.doi.org/10.1111/imm.13337DOI Listing
April 2021

Cigarette smoking and all-cause mortality in rural Chinese male adults: 15-year follow-up of the Anqing cohort study.

BMC Public Health 2021 Apr 9;21(1):696. Epub 2021 Apr 9.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.

Background: According to the Global Burden of Disease Study 2017, smoking is one of the leading four risk factors contributing to deaths in China. We aimed to evaluate the associations of smoking with all-cause mortality in a Chinese rural population.

Methods: Male participants over age 45 (n = 5367) from a large familial aggregation study in rural China, were included in the current analyses. A total of 528 former smokers and 3849 current smokers accounted for 10 and 71.7% of the cohort, respectively. Generalized Estimating Equations were used to evaluate the association between baseline smoking status and mortality, adjusting for pertinent covariates.

Results: There were 579 recorded deaths during the 15-year follow-up. Current smokers (odds ratio [OR],1.60; 95% CI,1.23-2.08) had higher all-cause mortality risks than nonsmokers. Relative to nonsmokers, current smokers of more than 40 pack-years ([OR],1.85; 95% CI,1.33-2.56) had a higher all-cause mortality risk. Compared to nonsmokers, current smokers who started smoking before age 20 ([OR],1.91; 95% CI,1.43-2.54) had a higher all-cause mortality risk, and former smokers in the lower pack-year group who quit after age 41 (median) ([OR],3.19; 95% CI,1.83-5.56) also had a higher risk of death after adjustment. Furthermore, former smokers who were also former drinkers had the highest significant risk of mortality than never smokers or drinkers. (P for interaction = 0.034).

Conclusions: This study provides evidence that current smokers and former smokers have a higher mortality risk than nonsmokers and would benefit from cessation at a younger age.
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http://dx.doi.org/10.1186/s12889-021-10691-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034075PMC
April 2021

PES inhibits human-inducible Hsp70 by covalent targeting of cysteine residues in the substrate-binding domain.

J Biol Chem 2020 Dec 24;296:100210. Epub 2020 Dec 24.

National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China; University of the Chinese Academy of Sciences, Beijing, China. Electronic address:

Hsp70 proteins are a family of ancient and conserved chaperones. They play important roles in vital cellular processes, such as protein quality control and the stress response. Hsp70 proteins are a potential drug target for treatment of disease, particularly cancer. PES (2-phenylethynesulfonamide or pifithrin-μ) has been reported to be an inhibitor of Hsp70. However, the mechanism of PES inhibition is still unclear. In this study we found that PES can undergo a Michael addition reaction with Cys-574 and Cys-603 in the SBDα of human HspA1A (hHsp70), resulting in covalent attachment of a PES molecule to each Cys residue. We previously showed that glutathionylation of Cys-574 and Cys-603 affects the structure and function of hHsp70. In this study, PES modification showed similar structural and functional effects on hHsp70 to glutathionylation. Further, we found that susceptibility to PES modification is influenced by changes in the conformational dynamics of the SBDα, such as are induced by interaction with adjacent domains, allosteric changes, and mutations. This study provides new avenues for development of covalent inhibitors of hHsp70.
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http://dx.doi.org/10.1074/jbc.RA120.015440DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7948744PMC
December 2020

Lipids and organic acids in three gut locations affect feed efficiency of commercial pigs as revealed by LC-MS-based metabolomics.

Sci Rep 2021 Apr 8;11(1):7746. Epub 2021 Apr 8.

College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangdong, 510642, China.

Feed efficiency (FE) is an important economic indicator in pig production. Improving the FE of commercial pigs is an important strategy for minimizing pig production costs and providing sustainability to the pig industry. In this study, nontargeted LC-MS metabolomics was performed on the contents of the three intestine segments (ileum, cecum and colon) of high-FE and low-FE pigs to explore the effects of small-molecule metabolites in pig intestine on pig FE. A total of 225 Duroc × (Landrace × Yorkshire) pigs in the 30-100 kg stage were sorted based on FE, and 20 pigs with extreme phenotypes were selected, with 10 in each group. A total of 749 metabolites were identified, of which 15, 38 and 11 differed between high-FE and low-FE pigs in ileum, cecum and colon, respectively. These candidate biomarkers mainly comprised lipids and organic acids, which could partially explain the FE difference between the two groups. Among the identified differential metabolites, the lipids are mainly involved in combatting inflammation and oxidation in the ileum and cecum and in bile acid metabolism and vitamin D absorption in the cecum. A difference in organic acids was mainly observed in the hindgut, which is involved in the metabolism of amino acids and fatty acids. This comprehensive study provides new insight into the biochemical mechanisms associated with pig FE.
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http://dx.doi.org/10.1038/s41598-021-87322-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032704PMC
April 2021

Fangchinoline suppresses conjunctival melanoma by directly binding FUBP2 and inhibiting the homologous recombination pathway.

Cell Death Dis 2021 Apr 7;12(4):380. Epub 2021 Apr 7.

State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.

Conjunctival melanoma (CM) is a rare and fatal ocular tumour with poor prognosis. There is an urgent need of effective therapeutic drugs against CM. Here, we reported the discovery of a novel potential therapeutic target for CM. Through phenotypic screening of our in-house library, fangchinoline was discovered to significantly inhibit the growth of CM cells including CM-AS16, CRMM1, CRMM2 and CM2005.1. Further mechanistic experiments indicated that fangchinoline suppressed the homologous recombination (HR)-directed DNA repair by binding with far upstream element binding protein 2 (FUBP2) and downregulating the expression of HR factors BRCA1 and RAD51. In vitro and in vivo antitumour experiments revealed that fangchinoline increased the efficacy of cisplatin by blocking HR factors and reduced the drug dose and toxicity. In conclusion, our work provides a promising therapeutic strategy for the treatment of CM that is worthy of extensive preclinical investigation.
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http://dx.doi.org/10.1038/s41419-021-03653-4DOI Listing
April 2021

Meta-analysis of alcohol consumption and venous thromboembolism.

J Public Health (Oxf) 2021 Apr 7. Epub 2021 Apr 7.

West China School of Nursing/West China Hospital, Sichuan University, Chengdu 610041, China.

Background: The associations of alcohol consumption and venous thromboembolism (VTE) have been investigated widely, but the conclusions were inconsistent.

Objective: To summarize the relationship of alcohol consumption and VTE.

Methods: This study has been registered in PROSPERO (ID: CRD42020164567). We searched the PubMed, Embase, Web of Science and the Cochrane Library databases from inception to September 2019 and reviewed the reference list of relevant articles to identify studies assessing the association between alcohol consumption and risk of VTE.

Results: Fourteen cohorts and four case-control studies were included in the meta-analysis. Compared with non-drinkers, the risk of VTE was decreased (RR: 0.93; 95% confidence interval [CI] 0.88-0.99) for alcohol drinkers. The pooled RRs of VTE were 0.91 (95% CI 0.84-0.99) for low to moderate alcohol intake (0.1-14.0 drinks/week) and 0.91 (95% CI 0.78-1.06) for high alcohol intake (>14.0 drinks/week) compared with non-drinker. Subgroup analysis showed liquor intake might slightly increase the risk of VTE (1.01; 95% CI 0.85-1.21) although the difference was not significant.

Conclusions: Alcohol consumption in low to moderate was associated with a lower risk of VTE. However, precautions are needed when providing personal drinking advice considering the potential harm of alcohol. Further studies are warranted to determine whether moderate alcohol consumption has a causal role in VTE.
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http://dx.doi.org/10.1093/pubmed/fdab045DOI Listing
April 2021

A preliminary world checklist of fern-mining insects.

Biodivers Data J 2021 25;9:e62839. Epub 2021 Mar 25.

Leafminer Group, School of Life Sciences, Gannan Normal University, Ganzhou, China Leafminer Group, School of Life Sciences, Gannan Normal University Ganzhou China.

Compared to the leaf-miners and stem-miners on flowering plants, the miners on ferns (including both Lycopodiophyta and Polypodiophyta in the broad sense) are less known. Knowledge of miners and their host plants is essential to fully understand plant-insect interactions. Although there are many scattered records on fern miners, a worldwide checklist has not been reported. We provide a preliminary checklist of fern-mining insects and their host plants worldwide. Altogether, we found records for 128 species and 18 families of fern miners, mainly that feed on Dennstaedtiaceae, Equisetaceae, Polypodiaceae and Aspleniaceae. Fern miners belonged to four orders: Diptera (51 species; 39.8%), Coleoptera (33 species; 25.8%), Lepidoptera (28 species; 21.9%) and Hymenoptera (16 species; 12.5%). They are primarily known from the Palaearctic Region, Nearctic Region, Neotropical Region and Oriental Region.
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http://dx.doi.org/10.3897/BDJ.9.e62839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016819PMC
March 2021

Very early neuroimages of sulfite oxidase deficiency mimicing severe hypoxic ischemic encephalopathy in a neonate.

Pediatr Neonatol 2021 Mar 18. Epub 2021 Mar 18.

Department of Neonatology, Guangdong Women and Children Hospital, Guang Zhou Medical University, Guangzhou, Guangdong, 510010, China. Electronic address:

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http://dx.doi.org/10.1016/j.pedneo.2021.03.005DOI Listing
March 2021

Construction and immunological evaluation of hepatitis B virus core virus-like particles containing multiple antigenic peptides of respiratory syncytial virus.

Virus Res 2021 Apr 2;298:198410. Epub 2021 Apr 2.

State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address:

Respiratory syncytial virus (RSV) infection causes severe disease in the lower respiratory tract of infants and young children. Currently, no licensed vaccine is available. In this study, we generated the chimeric virus-like particles (tHBc/F, tHBc/F/M2 and tHBc/F/M2/tG VLPs) containing multiple antigenic peptides of RSV proteins based on a truncated hepatitis B virus core carrier (tHBc). We investigated the immune protection against RSV infection induced by these VLPs in a mouse model. Immunization with the VLPs elicited RSV-specific IgG and neutralizing antibody production and conferred protection against RSV infection in vivo. Compared with UV-RSV or tHBc/F/M2/tG VLPs, the tHBc/F and tHBc/F/M2 VLPs induced significantly decreased Th2 cytokines (IL-4, IL-5) and increased Th1 cytokines (IFN-γ, TNF-α, IL-2) as well as increased IgG2a/IgG1 ratios. tHBc/F and tHBc/F/M2 VLPs also elicited an increased regulatory T (Treg) cell frequency and IL-10 secretion in the lungs of vaccinated mice, thereby relieving pulmonary pathology upon subsequent RSV infection. Our results demonstrate that the VLPs containing antigenic peptides of F protein combined with a CTL epitope of M2 may represent a promising RSV subunit vaccine candidate.
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http://dx.doi.org/10.1016/j.virusres.2021.198410DOI Listing
April 2021

Influences of advanced glycosylation end products on the inner blood-retinal barrier in a co-culture cell model .

Open Life Sci 2020 24;15(1):619-628. Epub 2020 Aug 24.

Department of Ophthalmology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, Sichuan Province, People's Republic of China.

Advanced glycosylation end products (AGEs) are harmful factors that can damage the inner blood-retinal barrier (iBRB). Rat retinal microvascular endothelial cells (RMECs) were isolated and cultured, and identified by anti-CD31 and von Willebrand factor polyclonal antibodies. Similarly, rat retinal Müller glial cells (RMGCs) were identified by H&E staining and with antibodies of glial fibrillary acidic protein and glutamine synthetase. The transepithelial electrical resistance (TEER) value was measured with a Millicell electrical resistance system to observe the leakage of the barrier. Transwell cell plates for co-culturing RMECs with RMGCs were used to construct an iBRB model, which was then tested with the addition of AGEs at final concentrations of 50 and 100 mg/L for 24, 48, and 72 h. AGEs in the iBRB model constructed by RMEC and RMGC co-culture led to the imbalance of the vascular endothelial growth factor (VEGF) and pigment epithelial derivative factor (PEDF), and the permeability of the RMEC layer increased because the TEER decreased in a dose- and time-dependent manner. AGEs increased VEGF but lowered PEDF in a dose- and time-dependent manner. The intervention with AGEs led to the change of the transendothelial resistance of the RMEC layer likely caused by the increased ratio of VEGF/PEDF.
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http://dx.doi.org/10.1515/biol-2020-0067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747502PMC
August 2020

Sex-Dependent Association Between Early Morning Ambulatory Blood Pressure Variations and Acute Mountain Sickness.

Front Physiol 2021 18;12:649211. Epub 2021 Mar 18.

Institute of Cardiovascular Diseases of PLA, The Second Affiliated Hospital, Third Military Medical University (Army Medical University), Chongqing, China.

Background: Acute high altitude (HA) exposure elicits blood pressure (BP) responses in most subjects, and some of them suffer from acute mountain sickness (AMS). However, a 24-h ambulatory BP (ABP) change and the correlation with the occurrence of AMS in different sexes are still unclear.

Objectives: This prospective study aimed to investigate HA induced BP responses in males and females and the relationship between AMS and 24-h ABP.

Methods: Forty-six subjects were matched according to demographic parameters by propensity score matching with a ratio of 1:1. All the subjects were monitored by a 24-h ABP device; the measurement was one period of 24 h BP. 2018 Lake Louise questionnaire was used to evaluate AMS.

Results: Both the incidence of AMS (14 [60.9%] vs. 5 [21.7%], = 0.007) and headache (18 [78.3%] vs. 8 [34.8%], = 0.003) were higher in females than in males. All subjects showed an elevated BP in the early morning [morning systolic BP (SBP), 114.72 ± 13.57 vs. 120.67 ± 11.10, = 0.013]. The elevation of morning SBP variation was more significant in females than in males (11.95 ± 13.19 vs. -0.05 ± 14.49, = 0.005), and a higher morning BP surge increase (4.69 ± 18.09 vs. -9.66 ± 16.96, = 0.005) was observed after acute HA exposure in the female group. The increase of morning SBP was associated with AMS occurrence ( = 0.662, < 0.001) and AMS score ( = 0.664, = 0.001). Among the AMS symptoms, we further revealed that the incidence ( = 0.786, < 0.001) and the severity of headache ( = 0.864, < 0.001) are closely correlated to morning SBP.

Conclusions: Our study demonstrates that females are more likely to suffer from AMS than males. AMS is closely associated with elevated BP in the early morning period, which may be correlated to higher headache incidence in subjects with higher morning SBP.
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http://dx.doi.org/10.3389/fphys.2021.649211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012890PMC
March 2021

CD47-mediated DTIC-loaded chitosan oligosaccharide-grafted nGO for synergistic chemo-photothermal therapy against malignant melanoma.

Mater Sci Eng C Mater Biol Appl 2021 Apr 5;123:112014. Epub 2021 Mar 5.

College of Chemistry and Material Science, Jinan University, Guangzhou, Guangdong 510632, PR China. Electronic address:

Nano-graphene oxide (nGO), an effective drug nanocarrier, is used for simultaneous photothermal therapy (PTT) and near-infrared fluorescence imaging. Dacarbazine (DTIC) is used in the treatment of melanoma with limited clinical efficacy. PTT shows promise in the treatment of skin cancer. Herein, chitosan oligosaccharide (COS)-grafted nGO was further modified with CD47 antibody, and loaded DTIC was prepared using a versatile nanoplatform (nGO-COS-CD47/DTIC) for the treatment of melanoma as a synergistic targeted chemo-photothermal therapy. The in vitro results demonstrated that nGO-COS-CD47/DTIC nanocarriers have excellent biocompatibility, photothermal conversion efficiency, high targeting efficiency, fast drug release under NIR irradiation, and tumor cell killing efficiency. Notably, nGO-COS-CD47/DTIC plus NIR irradiation significantly promoted early cell apoptosis through the mitochondrial apoptosis pathway and exhibited a significant joint function of antitumor efficacy. The demonstrated nGO-COS-CD47/DTIC can provide a highly efficient malignant melanoma therapy using this multifunctional intelligent nanoplatform.
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http://dx.doi.org/10.1016/j.msec.2021.112014DOI Listing
April 2021

Genome Mining, Heterologous Expression, Antibacterial and Antioxidant Activities of Lipoamides and Amicoumacins from Compost-Associated fmb60.

Molecules 2021 Mar 26;26(7). Epub 2021 Mar 26.

College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.

fmb60, which has broad-spectrum antimicrobial activities, was isolated from plant straw compost. A hybrid NRPS/PKS cluster was screened from the genome. Sixteen secondary metabolites produced by the gene cluster were isolated and identified using LC-HRMS and NMR. Three lipoamides D-F (-) and two amicoumacin derivatives, amicoumacins D, E (, ), were identified, and are reported here for the first time. Lipoamides D-F exhibited strong antibacterial activities against harmful foodborne bacteria, with the MIC ranging from 6.25 to 25 µg/mL. Amicoumacin E scavenged 38.8% of ABTS radicals at 1 mg/mL. Direct cloning and heterologous expression of the NRPS/PKS and gene cluster identified its importance for the biosynthesis of amicoumacins. This study demonstrated that there is a high potential for biocontrol utilization of fmb60, and genome mining for clusters of secondary metabolites of fmb60 has revealed a greater biosynthetic potential for the production of novel natural products than previously anticipated.
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http://dx.doi.org/10.3390/molecules26071892DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036425PMC
March 2021