Publications by authors named "Jie Yang"

4,435 Publications

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Exposure to Benzo(a)pyrene damages mitochondrial function via suppressing mitochondrial melatonin receptors in ovarian corpus luteum during early pregnancy.

Chem Biol Interact 2022 Aug 5:110085. Epub 2022 Aug 5.

Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Chongqing, 400016, PR China; Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address:

Benzo(a)pyrene (BaP) is a well-known environmental endocrine pollutant, which has ovarian toxicity in mammals. Ovarian corpus luteum (CL), as the main source of progesterone synthesis in early pregnant female, requires a large number of mitochondria for energy supply. We previously demonstrated that BaP and its metabolite benzo(a)pyren-7, 8-dihydrodiol-9, 10-epoxide (BPDE) inhibited the ovarian melatonin receptors (MTRs) expression and decreased the levels of estrogen and progesterone during early pregnancy in mice. Emerging researches show that MTRs also exist on mitochondrial membrane and participate in the regulation of mitochondrial function. However, the relationship between BaP, MTRs on mitochondrial membrane and mitochondrial function remains unknown. Consequently, this study focuses on the effect and potential mechanism of BaP on ovarian luteal mitochondrial function during early pregnancy. We found that BaP and its metabolite BPDE decreased MTRs in early pregnant CL and luteinized KGN cells, especially in mitochondria. Furthermore, BaP or BPDE up-regulated the expression of SIRT3, Mfn2 and Drp-1, damaged mitochondrial morphology and decreased the MMP and the ATP levels, thereby causing mitochondrial dysfunction. Notably, activation of the MTRs on mitochondrial membrane by MTRs agonist ramelteon partially alleviated BPDE-induced up-regulation of SIRT3, Mfn2 and Drp-1, reduced mitochondrial fragmentation and enhanced the MMP and the ATP levels, thus restoring the expression of steroid rate-limiting enzymes. Together, these findings firstly proved that BaP and BPDE down-regulate MTRs on mitochondrial membrane, and further injure mitochondrial function in early pregnant rats' CL, which provides a new insight for understanding the exact mechanism of the BaP-induced ovarian toxicity.
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http://dx.doi.org/10.1016/j.cbi.2022.110085DOI Listing
August 2022

Three water-soluble acylhydrazone tetranuclear transition metal complexes: Crystal structures, DNA/BSA interactions and cytotoxicity studies.

J Inorg Biochem 2022 Jul 26;236:111941. Epub 2022 Jul 26.

College of Chemistry and Chemical Engineering, Xi'an University of Science and Technology, Xi'an 710054, PR China.

2-acetylpyridine-4-chloropyridine-2‑carbonyl hydrazone (CHClNO, HL) and its three water-soluble tetranuclear complexes [Cu(NO)(L)]·(NO) (1), [Co(NO)(HO)(CHOH)(L)]·(NO) (2) and [Zn(NO)(HO)(CHOH)(L)]·(NO) (3) were synthesized and characterized showing that 1-3 were all tetranuclear complexes. The interactions of HL, 1-3 with calf thymus DNA (CT-DNA) and bovine serum albumin (BSA) were explored using ultraviolet-visible (UV-Vis) titration, fluorescence spectroscopy, microcalorimetry and molecular docking techniques. The UV-Vis spectroscopy measurements showed that complexes 1-3 could strongly bind to CT-DNA by the intercalation mode, while HL interacted with CT-DNA through groove binding. From the fluorescence spectroscopy results, the interaction between HL, 1-3 and BSA was a static quenching procedure, in which complexes 1-3 had two binding sites near Trp residues of BSA while HL only had one. The microcalorimetric studies revealed that the interactions of HL and 1-3 to CT-DNA/BSA were all endothermic and the duration of each interaction was all less than 30 min. The in silico molecular docking illustrated intermolecular interactions of 1-3 binding with DNA/BSA included hydrogen bond, halogen bond, hydrophobic and electrostatic interactions. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay indicated that complex 1 possessed better cytotoxicity against HeLa, A549, MCF7 and HCT-116 than cisplatin and could be used as an alternative anticancer drug.
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http://dx.doi.org/10.1016/j.jinorgbio.2022.111941DOI Listing
July 2022

CRISPR-Cas in Acinetobacter baumannii Contributes to Antibiotic Susceptibility by Targeting Endogenous .

Microbiol Spectr 2022 Aug 8:e0082922. Epub 2022 Aug 8.

Department of Microbiology, Institute of Translational Medicine, Medical College, Yangzhou Universitygrid.268415.c, Yangzhou, PR China.

Acinetobacter baumannii is a well-known human opportunistic pathogen in nosocomial infections, and the emergence of multidrug-resistant Acinetobacter baumannii has become a complex problem for clinical anti-infective treatments. The ways this organism obtains multidrug resistance phenotype include horizontal gene transfer and other mechanisms, such as altered targets, decreased permeability, increased enzyme production, overexpression of efflux pumps, metabolic changes, and biofilm formation. A CRISPR-Cas system generally consists of a CRISPR array and one or more operons of genes, which can restrict horizontal gene transfer in bacteria. Nevertheless, it is unclear how CRISPR-Cas systems regulate antibiotic resistance in Acinetobacter baumannii. Thus, we sought to assess how CRISPR-Cas affects biofilm formation, membrane permeability, efflux pump, reactive oxygen species, and quorum sensing to clarify further the mechanism of CRISPR-Cas regulation of Acinetobacter baumannii antibiotic resistance. In the clinical isolate AB43, which has a complete I-Fb CRISPR-Cas system, we discovered that the Cas3 nuclease of this type I-F CRISPR-Cas system regulates Acinetobacter baumannii quorum sensing and has a unique function in changing drug resistance. As a result of quorum sensing, synthase is reduced, allowing efflux pumps to decrease, biofilm formation to become weaker, reactive oxygen species to generate, and drug resistance to decrease in response to CRISPR-Cas activity. These observations suggest that the CRISPR-Cas system targeting endogenous may boost bacterial antibiotic sensitivity. CRISPR-Cas systems are vital for genome editing, bacterial virulence, and antibiotic resistance. How CRISPR-Cas systems regulate antibiotic resistance in Acinetobacter baumannii is almost wholly unknown. In this study, we reveal that the quorum sensing regulator mRNA was a primary target of the I-Fb CRISPR-Cas system and the cleavage activity of Cas3 was the most critical factor in regulating mRNA degradation. These results advance our understanding of how CRISPR-Cas systems inhibit drug resistance. However, the mechanism of endogenous targeting of by CRISPR-Cas needs to be further explored.
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http://dx.doi.org/10.1128/spectrum.00829-22DOI Listing
August 2022

[Effect of acupuncture on brain functional connectivity strength in patients with primary dysmenorrhea].

Zhongguo Zhen Jiu 2022 Aug;42(8):863-70

College of Acupuncture-Moxibustion and Tuina, Chengdu University of TCM, Chengdu 610075, Sichuan Province, China; Clinical Research Center for Acupuncture and Moxibustion in Sichuan Province, Chengdu 610075.

Objective: To investigate the effect of acupuncture on the brain functional activities of the patients with primary dysmenorrhea based on the resting-state functional magnetic resonance imaging (rs-fMRI), and to provide visual evidence for the central mechanism of acupuncture in treatment of primary dysmenorrhea.

Methods: Forty-two patients of primary dysmenorrhea were enrolled and randomly divided into an observation group (21 cases, 1 case dropped off) and a control group (21 cases, 2 cases dropped off, 3 cases withdrawal). In the observation group, acupuncture was exerted at Sanyinjiao (SP 6) and Guanyuan (CV 4), started 5-7 days before menstrual flow, once a day till menstrual onset, for a total of 3 menstrual cycles. No intervention was applied in the control group. The scores of visual analogue scale (VAS) and Cox menstrual symptom scale (CMSS) were observed in both groups before and after treatment. Based on rs-fMRI, the data of resting-state functional magnetic resonance were collected from two groups before and after treatment. Combined with functional connectivity strength (FCS) and functional connectivity (FC) analysis, the differences of brain regions before and after treatment were compared between the two groups and the correlation was analyzed between their functional connectivity changes and the improvements in VAS and CMSS scores of the patients in the observation group.

Results: In the observation group, the scores of VAS and CMSS were all decreased after treatment (<0.05), while the scores related to the symptom time in CMSS was reduced in comparison with that before treatment in the control group (<0.05). The score reducing ranges of VAS and CMSS in the observation group were larger than the control group (<0.05). Compared before treatment, FCS of the right middle cingulate cortex and the left cuneus was increased, while FCS of the left inferior parietal lobule was decreased after treatment in the observation group. In the control group, FCS of the left orbital frontal cortex was increased after treatment. Compared with the control group, FCS of the left anterior insula was increased in the observation group after treatment. FC analysis was performed using the left anterior insula as the seed point. In comparison with the control group, FC of the left anterior insula was increased either with the inferior temporal gyrus or with the right hippocampus; and was decreased either with the middle occipital lobe or with the right dorsolateral prefrontal cortex in the observation group after treatment. In the observation group, FC between the left anterior insula and the right hippocampus was positively correlated with the improvements in symptom severity ( =0.385, <0.05) and symptom time ( =0.510, <0.05) of CMSS, and FC between the right dorsolateral prefrontal cortex and the left anterior insula was negatively correlated with the improvement in symptom severity of CMSS after treatment ( =-0.373, <0.05).

Conclusion: The anterior insula may be the key brain region in treatment of primary dysmenorrhea with acupuncture. Acupuncture may relieve dysmenorrhea and the related symptoms through strengthening the functional connectivity of anterior insula-limbic system and anterior insula-control network.
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http://dx.doi.org/10.13703/j.0255-2930.20210826-k0004DOI Listing
August 2022

Altered Regional Homogeneity in Patients With Diabetic Erectile Dysfunction: A Resting-State fMRI Study.

Front Endocrinol (Lausanne) 2022 22;13:817523. Epub 2022 Jul 22.

Department of Andrology, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.

Introduction: Erectile dysfunction (ED) is a common complication of Type-2 Diabetes Mellitus (T2DM) for male patients and it is considered to be associated with different causes including hyperglicemia-induced vascular endothelial cell damages. However, the possible central neural mechanisms shared by these two diseases remain unclear. This study aimed to explore the changes of brain activity and their relationships with the clinical characteristics in patients with diabetic ED.

Methods: The data of resting-state functional magnetic resonance imaging were acquired in 31 T2DM patients with ED (DM-ED) and 31 matched healthy controls (HCs). The whole-brain regional homogeneity (ReHo) values were calculated and compared between groups. In addition, correlation analysis was performed to evaluate the relationships between brain regions with altered ReHo values and clinical characteristics in the patient group.

Results: The DM-ED group exhibited increased ReHo values in the right middle frontal gyrus (orbital part) and decreased ReHo values in the left superior frontal gyrus (dorsolateral), paracentral lobule, precuneus and bilateral supplementary motor area when compared with the HCs group. Moreover, significantly negative correlations were found between ReHo values of the left superior frontal gyrus (dorsolateral) and IIEF-5 scores, as well as the level of HbA1c in the DM-ED group.

Conclusion: The altered spontaneous brain activity in cognitive-related regions revealed by ReHo values might provide new insights into the neurological pathophysiology underlying DM-ED and serve as potential neuroimaging biomarkers for detecting and evaluating ED in diabetes patients.
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http://dx.doi.org/10.3389/fendo.2022.817523DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355575PMC
July 2022

Dexamethasone-loaded zeolitic imidazolate frameworks nanocomposite hydrogel with antibacterial and anti-inflammatory effects for periodontitis treatment.

Mater Today Bio 2022 Dec 20;16:100360. Epub 2022 Jul 20.

Department of Stomatology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University, 200025, Shanghai, China.

Periodontitis is a bacterial-induced, chronic inflammatory disease characterized by progressive destruction of tooth-supporting structures. Pathogenic bacteria residing in deep periodontal pockets after traditional manual debridement can still lead to local inflammatory microenvironment, which remains a challenging problem and an urgent need for better therapeutic strategies. Here, we integrated the advantages of metal-organic frameworks (MOFs) and hydrogels to prepare an injectable nanocomposite hydrogel by incorporating dexamethasone-loaded zeolitic imidazolate frameworks-8 (DZIF) nanoparticles into the photocrosslinking matrix of methacrylic polyphosphoester (PPEMA) and methacrylic gelatin (GelMA). The injectable hydrogel could be easily injected into deep periodontal pockets, achieving high local concentrations without leading to antibiotic resistance. The nanocomposite hydrogel had high antibacterial activity and constructs with stable microenvironments maintain cell viability, proliferation, spreading, as well as osteogenesis, and down-regulated inflammatory genes expression . When evaluated on an experimental periodontitis rat model, micro-computed tomography and histological analyses showed that the nanocomposite hydrogel effectively reduced periodontal inflammation and attenuated inflammation-induced bone loss in a rat model of periodontitis. These findings suggest that the nanocomposite hydrogel might be a promising therapeutic candidate for treating periodontal disease.
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http://dx.doi.org/10.1016/j.mtbio.2022.100360DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352959PMC
December 2022

The Effects of Airway Pressure Release Ventilation on Pulmonary Permeability in Severe Acute Respiratory Distress Syndrome Pig Models.

Front Physiol 2022 22;13:927507. Epub 2022 Jul 22.

Department of Critical Care Medicine, West China Hospital of Sichuan University, Chengdu, China.

The aim of the study was to compare the effects of APRV and LTV ventilation on pulmonary permeability in severe ARDS. Mini Bama adult pigs were randomized into the APRV group ( = 5) and LTV group ( = 5). A severe ARDS animal model was induced by the whole lung saline lavage. Pigs were ventilated and monitored continuously for 48 h. Compared with the LTV group, CStat was significantly better ( < 0.05), and the PaO/FiO ratio showed a trend to be higher throughout the period of the experiment in the APRV group. The extravascular lung water index and pulmonary vascular permeability index showed a trend to be lower in the APRV group. APRV also significantly mitigates lung histopathologic injury determined by the lung histopathological injury score ( < 0.05) and gross pathological changes of lung tissues. The protein contents of occludin ( < 0.05), claudin-5 ( < 0.05), E-cadherin ( < 0.05), and VE-cadherin ( < 0.05) in the middle lobe of the right lung were higher in the APRV group than in the LTV group; among them, the contents of occludin ( < 0.05) and E-cadherin ( < 0.05) of the whole lung were higher in the APRV group. Transmission electron microscopy showed that alveolar-capillary barrier damage was more severe in the middle lobe of lungs in the LTV group. In comparison with LTV, APRV could preserve the alveolar-capillary barrier architecture, mitigate lung histopathologic injury, increase the expression of cell junction protein, improve respiratory system compliance, and showed a trend to reduce extravascular lung water and improve oxygenation. These findings indicated that APRV might lead to more profound beneficial effects on the integrity of the alveolar-capillary barrier architecture and on the expression of biomarkers related to pulmonary permeability.
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http://dx.doi.org/10.3389/fphys.2022.927507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354663PMC
July 2022

Highly efficient Hg removal via a competitive strategy using a Co-based metal organic framework ZIF-67.

J Environ Sci (China) 2022 Sep 14;119:33-43. Epub 2022 Jan 14.

School of Resources and Environmental Engineering, State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai 200237, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address:

The stronger coordination ability of mercury ions with organic ligands than the metal ions in metal organic framework (MOFs) provides an accessible way to separate mercury ions from solution using specific MOFs. In this study, a Co-based MOF (ZIF-67, Co(mIM)) was synthesized. It did not introduce specific functional groups, such as -SH and -NH, into its structure through complicated steps. It separate Hg from wastewater with a new strategy, which utilized the stronger coordination ability of Hg with the nitrogen atom on the imidazole ring of the organic ligand than the Co ions. Hg replaced Co nodes from ZIF-67 and formed a more stable precipitate with mIM. The experimental results showed that this new strategy was efficient. ZIF-67 exhibited Hg adsorption capacity of 1740 mg/g, much higher than the known MOFs sorbents. mIMs is the reaction center and ZIF-67 can improve its utilization. The sample color faded from purple to white due to the loss of cobalt ion. It is a great feature of ZIF-67 that allows users to judge whether the sorbent is deactivated intuitively. ZIF-67 can be sustainable recycled by adding organic ligands to the solution after treatment due to its simple synthesis method at room temperature. It's a high-efficient and sustainable sorbent for Hg separation from wastewater.
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http://dx.doi.org/10.1016/j.jes.2021.08.032DOI Listing
September 2022

Characteristics and molecular identification of glyceraldehyde-3-phosphate dehydrogenases in poplar.

Int J Biol Macromol 2022 Aug 3;219:185-198. Epub 2022 Aug 3.

Key Laboratory of Landscape Plant Genetics and Breeding, School of Life Sciences, Nantong University, Nantong, China. Electronic address:

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), an essential enzyme of the glycolysis metabolic pathway, plays a vital role in carbon metabolism, plant development, and stress resistance. As a kind of woody plant, poplars are widely cultivated for afforestation. Although the whole genome data of poplars have been published, little information is known about the GAPDH family of genes in poplar. This study performed a genome-wide identification of the poplar GAPDH family, and 13 determined PtGAPDH genes were identified from poplar genome. Phylogenetic tree showed that the PtGAPDH members were divided into PtGAPA/B, PtGAPC, PtGAPCp, and PtGAPN groups. A total of 13 PtGAPDH genes were distributed on eight chromosomes, 13 gene pairs belonging to segmented replication events were detected in poplar, and 23 collinearity gene pairs were determined between poplar and willow. The PtGAPDHcis-acting elements associated with growth and development as well as stress resistance revealed that PtGAPDHs might be involved in these processes. The phosphoglycerate kinase (PGK) and triose-phosphate isomerase (TPI) were predicted as the putative interaction proteins of PtGAPDHs. Gene ontology (GO) analysis showed that PtGAPDHs play a crucial role in the oxidation and reduction processes. PtGAPDH expression levels were induced by NaCl and PEG treatments, which implied that PtGAPDHs might be involved in stress response. Overexpression of PtGAPC1 significantly changed the contents of lipid and carbohydrate metabolites, which indicated that PtGAPC1 plays an essential role in metabolic regulation. This study highlights the characterizations and profiles of PtGAPDHs and reveals that PtGAPC1 is involved in the loop of lipid and carbohydrate metabolisms.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.08.001DOI Listing
August 2022

Prediction of recurrent spontaneous abortion using evolutionary machine learning with joint self-adaptive sime mould algorithm.

Comput Biol Med 2022 Jul 26;148:105885. Epub 2022 Jul 26.

Department of Obstetrics and Gynecology, The Third Clinical Institute Affiliated to Wenzhou Medical University, Wenzhou People's Hospital, Wenzhou 325000, China. Electronic address:

Recurrent spontaneous abortion (RSA) is a frequent abnormal pregnancy with long-term psychological repercussions that disrupt the peace of the whole family. In the diagnosis and treatment of RSA worsened by thyroid disorders, recurrent spontaneous abortion is also a significant obstacle. The pathogenesis and possible treatment methods for RSA are yet unclear. Using clinical information, vitamin D and thyroid function measurements from normal pregnant women with RSA, we attempt to build a framework for conducting an effective analysis for RSA in this research. The framework is presented by combining the joint self-adaptive sime mould algorithm (JASMA) with the common kernel learning support vector machine with maximum-margin hyperplane theory, abbreviated as JASMA-SVM. The JASMA has a complete set of adaptive parameter change methods, which improves the algorithm's global search and optimization capabilities and guarantees that it speeds convergence and departs from the local optimum. On CEC 2014 benchmarks, the property of JASMA is validated, and then it is utilized to concurrently optimize parameters and select optimal features for SVM on RSA data from VitD, thyroid hormone levels, and thyroid autoantibodies. The statistical results demonstrate that the proposed JASMA-SVM can be treated as a potential tool for RSA with accuracy of 92.998%, MCC of 0.92425, sensitivity of 93.286%, specificity of 93.064%.
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http://dx.doi.org/10.1016/j.compbiomed.2022.105885DOI Listing
July 2022

Comparative evaluation of production performances of cloned pigs derived from superior Duroc boars.

Anim Reprod Sci 2022 Jul 28;244:107049. Epub 2022 Jul 28.

National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. Electronic address:

Since pig was successfully cloned in 2000, somatic cell nuclear transfer (SCNT) became a promising technique in preserving and expanding the genetics of superior boars. Assessing the safety, growth performance, and reproductive performance of cloned pigs and their progeny is critical for their wide application. In this study, three superior Duroc boars were used to construct 61,736 SCNT-cloned embryos. The semen quality and reproductive performance of the cloned Duroc pigs and the growth performances of their progeny were evaluated. Results showed that the cloned pigs derived from superior boars produced semen with normal quality and exhibited similar reproductive performance as the donor boars, whose progenies showed greater growth performance than those derived from non-cloned pigs under the same feed condition. The results shed light on the application of cloning technology in the conservation and expansion of the genetic resources of Duroc pigs.
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http://dx.doi.org/10.1016/j.anireprosci.2022.107049DOI Listing
July 2022

Delta-like 4-Derived Notch Signals Differentially Regulate Thymic Generation of Skin-Homing CCR10NK1.1 Innate Lymphoid Cells at Neonatal and Adult Stages.

J Immunol 2022 Aug 3. Epub 2022 Aug 3.

Department of Microbiology, Immunology and Molecular Genetics, University of Texas Health Science Center San Antonio, San Antonio, TX;

The thymus is a primary lymphoid organ for T cell development. Increasing evidence found that the thymus is also an important site for development of innate lymphoid cells (ILCs). ILCs generated in thymi acquire unique homing properties that direct their localization into barrier tissues such as the skin and intestine, where they help local homeostasis. Mechanisms underlying the developmental programming of unique tissue-homing properties of ILCs are poorly understood. We report in this article that thymic stroma-derived Notch signaling is differentially involved in thymic generation of a population of NK1.1 group 1 ILCs (ILC1s) with the CCR10 skin-homing property in adult and neonatal mice. We found that thymic generation of CCR10NK1.1 ILC1s is increased in T cell-deficient mice at adult, but not neonatal, stages, supporting the notion that a large number of developing T cells interfere with signals required for generation of CCR10NK1.1 ILC1s. In an in vitro differentiation assay, increasing Notch signals promotes generation of CCR10NK1.1 ILC1s from hematopoietic progenitors. Knockout of the Notch ligand Delta-like 4 in thymic stroma impairs generation of CCR10NK1.1 ILC1s in adult thymi, but development of CCR10NK1.1 ILC1s in neonatal thymi is less dependent on Delta-like 4-derived Notch signals. Mechanistically, the Notch signaling is required for proper expression of the IL-7R CD127 on thymic NK1.1 ILC1s, and deficiency of CD127 also impairs thymic generation of CCR10NK1.1 ILC1s at adult, but not perinatal, stages. Our findings advanced understanding of regulatory mechanisms of thymic innate lymphocyte development.
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http://dx.doi.org/10.4049/jimmunol.2100870DOI Listing
August 2022

Drivers of spatial and seasonal variations of CO and CH fluxes at the sediment water interface in a shallow eutrophic lake.

Water Res 2022 Jul 28;222:118916. Epub 2022 Jul 28.

Inner Mongolia Key Laboratory of River and Lake Ecology, School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China; Department of Geography, National University of Singapore, 117570, Singapore.

Shallow eutrophic lakes contribute disproportional to the emissions of CO and CH from inland waters. The processes that contribute to these fluxes, their environmental controls, and anthropogenic influences, however, are poorly constrained. Here, we studied the spatial variability and seasonal dynamics of CO and CH fluxes across the sediment-water interface, and their relationships to porewater nutrient concentrations in Lake Ulansuhai, a shallow eutrophic lake located in a semi-arid region in Northern China. The mean concentrations of CO and CH in porewater were 877.8 ± 31.0 µmol L and 689.2 ± 45.0 µmol L, which were more than 50 and 20 times higher than those in the water column, respectively. The sediment was always a source of both gases for the water column. Porewater CO and CH concentrations and diffusive fluxes across the sediment-water interface showed significant temporal and spatial variations with mean diffusive fluxes of 887.3 ±124.7 µmol m d and 607.1 ± 68.0 µmol m d for CO and CH, respectively. The temporal and spatial variations of CO and CH concentrations in porewater were associated with corresponding variations in dissolved organic carbon and dissolved nitrogen species. Temperature and dissolved organic carbon in surface porewater were the most important drivers of temporal variations in diffusive fluxes, whereas dissolved organic carbon and nitrogen were the main drivers of their spatial variations. Diffusive fluxes generally increased with increasing dissolved organic carbon and nitrogen in the porewater from the inflow to the outflow region of the lake. The estimated fluxes of both gases at the sediment-water interface were one order of magnitude lower than the emissions at the water surface, which were measured in a companion study. This indicates that diffusive fluxes across the sediment-water interface were not the main pathway for CO and CH emissions to the atmosphere. To improve the mechanistic understanding and predictability of greenhouse gas emissions from shallow lakes, future studies should aim to close the apparent gap in the CO and CH budget by combining improved flux measurement techniques with process-based modeling.
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http://dx.doi.org/10.1016/j.watres.2022.118916DOI Listing
July 2022

Triboelectric Patch Based on Maxwell Displacement Current for Human Energy Harvesting and Eye Movement Monitoring.

ACS Nano 2022 Aug 3. Epub 2022 Aug 3.

Research Center for Bioengineering and Sensing Technology, Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, Beijing Municipal Key Laboratory of New Energy Materials and Technologies, University of Science and Technology Beijing, Beijing 100083, China.

The forthcoming wearable health care devices garner considerable attention because of their potential for monitoring, treatment, and protection applications. Herein, a self-powered triboelectric patch was developed using polytetrafluoroethylene rubbed with nylon fabric. The triboelectric patch can maintain a stable electrostatic field, due to the excess electrification on the surface of the triboelectric layer. The designed triboelectric nanogenerator (TENG) output watt density can reach about 485 mW/m with added resistance of 11 kΩ. Additionally, the performance of the triboelectric patch allowed eye movement monitoring. The maximum voltage could reach 80 V at the vertical distance of 20 mm between the frictional layer and collector. The triboelectric patch not only can power a digital watch for potential wearable applications but also can be integrated to monitor eye movements during sleep. This work proposed a mechanism for human movement energy harvesting, which may be used for self-powered smart wearable health equipment and Maxwell displacement current wireless sensors.
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http://dx.doi.org/10.1021/acsnano.2c01199DOI Listing
August 2022

The influence of unexpected early termination of intravenous rt-PA treatment on clinical outcome in acute ischemic stroke patients.

Acta Neurol Belg 2022 Aug 2. Epub 2022 Aug 2.

Department of Neurology, School of Medicine, Tongji University, East Hospital, No. 150 Jimo Road, Shanghai, 200120, People's Republic of China.

Introduction: This study aimed to explore the impact of unexpected early termination during intravenous thrombolysis on clinical prognosis in patients with acute ischemic stroke (AIS).

Methods: Patients who received intravenous thrombolysis were divided into an early termination group and a normal treatment group. The causes of unexpected termination were analyzed, and the prognosis was compared between the groups.

Results: The main causes of early termination of thrombolytic therapy included subjective wishes of family members (11.8%, 4) and persistently elevated blood pressure (14.7%, 5). The effective rate of thrombolytic therapy in the early termination group was significantly lower than that in the normal treatment group (P < 0.05). The rate of early neurological deterioration in the early termination group was significantly higher than that in the normal treatment group (P < 0.05). There was no significant difference in the incidence of symptomatic intracranial hemorrhage after thrombolysis between the two groups (P > 0.05). The average mRS score of the early termination group was significantly higher than that of the normal treatment group (P < 0.05). Multivariate analysis indicated that early termination of thrombolytic therapy and cumulative dosage of rt-PA before termination were the main factors affecting the 3-month prognosis.

Conclusion: Subjective wishes of family members and persistently elevated blood pressure may be the main causes of early termination of thrombolysis, and the 3-month prognosis of patients could be adversely affected by early termination of thrombolytic therapy and cumulative dosage of rt-PA. Certain measures, such as popularizing thrombolytic health education and optimizing blood pressure management before and during thrombolysis, may be helpful for the normal operation of intravenous thrombolysis.
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http://dx.doi.org/10.1007/s13760-022-02042-8DOI Listing
August 2022

An Intelligent Nanomachine Guided by DNAzyme Logic System for Precise Chemodynamic Therapy.

Angew Chem Int Ed Engl 2022 Aug 1. Epub 2022 Aug 1.

Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, 5625 Renmin Street, 130022, Changchun, CHINA.

The intelligent nanomachine usually has a control center to carry out self-regulation. Unfortunately, most of the nanomaterials for chemodynamic therapy (CDT) do not have such a control center to sense and process the chemical or biological signals, which greatly weakens the selectivity and efficiency of CDT. To address this problem, here an intelligent nanomachine was constructed with a DNAzyme logic gate as the control center, and metal organic framework as the actuator. The well-designed nanomachine showed an enhanced killing effect on cancer cells but posed no harm to normal cells, acquiring better selectivity than clinical chemotherapy drugs (doxorubicin and cisplatin). To the best of our knowledge, this is the first reported cell-specific CDT by the guidance of DNAzyme logic gate. Our work highlights the great potential of DNAzymes in intelligent response networks, and extends the implementation of nanomachines in precision medicine.
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http://dx.doi.org/10.1002/anie.202204291DOI Listing
August 2022

Five-Year Outcomes After Endovascular Treatment for Large Vessel Occlusion Stroke.

Front Neurosci 2022 13;16:920731. Epub 2022 Jul 13.

Department of Neurology, The Third People's Hospital of Zigong, Zigong, China.

Background: The long-term outcomes of acute large vessel occlusion (LVO) in anterior circulation treated by endovascular treatment (EVT) remains to be determined. The aim of this study was to assess the 5-year outcomes of patients with LVO who underwent EVT.

Methods: This study was an observational, nationwide registry of consecutive patients with acute LVO who received EVT in 28 comprehensive stroke centers in China. The primary outcome was the proportion of favorable outcome [modified Rankin Scale score (mRS) 0-2] at 5 years. Secondary outcomes included proportions of patients with excellent outcome (mRS 0-1), all-cause mortality and risk of stroke recurrence at 5 years.

Results: A total of 807 patients were included into the study and had 90-day follow-up data, 657 patients had 5-year follow-up data. At 90 days, 218 patients (27.0%) had an excellent outcome, 349 patients (43.2%) had a favorable functional outcome. 199 patients (24.7%) died. At 5 years, 190 patients (28.9%) had an excellent outcome, 261 patients (39.7%) had a favorable functional outcome, 317 patients (48.2%) died and 129 (28.2%) had stroke recurrence. Because of missing 5-year follow-up data, among available 269 patients who achieved functional independence at 90 days, 208 (77.3%) maintained favorable outcome, 19 (7.1%) had disability (mRS 3-5) and 42 (15.6%) died at 5 years. Furthermore, among available 189 patients with mRS 3-5 at 90 days, 53 (28.0%) patients achieved favorable functional outcome, 60 (31.7%) patients maintained unfavorable functional outcome and 76 (40.2%) patients died within 5 years. Multivariate analyses identified that younger age [odds ratio (OR): 0.96; 95% CI, 0.93-0.99; = 0.009], lower mRS at 90 days (OR: 0.15; 95% CI, 0.10-0.23; < 0.001) and absence of stroke recurrence (OR: 0.001; 95% CI, 0.000-0.006; < 0.001) were significantly associated with favorable outcome at 5 years. Advanced age (OR: 1.06, 95% CI, 1.04-1.08; < 0.001), higher mRS at 90 days (OR: 0.84; 95% CI, 0.73-0.98; = 0.021) and atrial fibrillation (OR: 1.63; 95% CI, 1.02-2.60; = 0.04) were independent factors for stroke recurrence.

Conclusion: Our results indicated that the beneficial effect of EVT in patients with acute LVO can be sustained during the course of at least 5 years. Reducing the risk of stroke recurrence by anticoagulation for atrial fibrillation may be a crucial strategy to improve long-term outcome.
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http://dx.doi.org/10.3389/fnins.2022.920731DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326078PMC
July 2022

Transcriptome and Metabolome Analyses Reveal the Involvement of Multiple Pathways in Flowering Intensity in Mango.

Front Plant Sci 2022 14;13:933923. Epub 2022 Jul 14.

Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, China.

Mango ( L.) is famous for its sweet flavor and aroma. China is one of the major mango-producing countries. Mango is known for variations in flowering intensity that impacts fruit yield and farmers' profitability. In the present study, transcriptome and metabolome analyses of three cultivars with different flowering intensities were performed to preliminarily elucidate their regulatory mechanisms. The transcriptome profiling identified 36,242 genes. The major observation was the differential expression patterns of 334 flowering-related genes among the three mango varieties. The metabolome profiling detected 1,023 metabolites that were grouped into 11 compound classes. Our results show that the interplay of the FLOWERING LOCUS T and CONSTANS together with their upstream/downstream regulators/repressors modulate flowering robustness. We found that both gibberellins and auxins are associated with the flowering intensities of studied mango varieties. Finally, we discuss the roles of sugar biosynthesis and ambient temperature pathways in mango flowering. Overall, this study presents multiple pathways that can be manipulated in mango trees regarding flowering robustness.
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http://dx.doi.org/10.3389/fpls.2022.933923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330041PMC
July 2022

Genome-wide survey of open chromatin regions in two swallowtail butterflies Papilio machaon and P. bianor.

Arch Insect Biochem Physiol 2022 Jul 31:e21952. Epub 2022 Jul 31.

State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.

Papilio machaon was assigned as the type species for all butterflies by Linnaeus and P. bianor is a congener but exhibits a great difference in morphology (especially larva and adult color pattern) and larval host plants from P. machaon. Thus, they are the ideal models to investigate genetic mechanisms underlying morphology and plasticity between congeners. The reference genomes of both species were dissected in our previous studies, but little is known about their regulatory genome and the epigenetic regulation of gene expression throughout developmental stages. Here, we profiled the chromatin accessibility and gene expression of three developmental stages (the 4th instar larva [L4], the 5th instar larva [L5], and pupa [P]) using transposase accessible chromatin sequencing (ATAC-seq) and RNA-seq. Results showed that many accessible chromatin peaks were identified at three developmental stages (peak number, P. machaon: 44,977 [L4], 36,919 [L5], 47,147 [P]; P. bianor: 20,341 [L4], 44,668 [L5], 62,249 [P]). Moreover, the number of differentially accessible peaks and differentially expressed genes between larval stages of each butterfly species are significantly fewer than that between larval and pupal stages, suggesting a higher similarity within larvae and a significant difference between larvae and pupae. This study added the annotated information of chromatin accessibility genome-wide of the two papilionid species and will promote the investigation of gene regulation in butterfly evolution.
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http://dx.doi.org/10.1002/arch.21952DOI Listing
July 2022

Spatial-temporal evolution of ESV and its response to land use change in the Yellow River Basin, China.

Sci Rep 2022 Jul 30;12(1):13103. Epub 2022 Jul 30.

College of Pratacultural Science, Gansu Agricultural University, Lanzhou, 730070, China.

The value of ecosystem services, as well as their temporal and spatial characteristics, can be used to help areas develop focused and localized sustainable ecological management plans. Thus, this study conducted in the Yellow River Basin (YRB) of China, analyzed the ecosystem service value (ESV) and its spatial-temporal variation characteristics. This study used the equivalent factor and geospatial exploration methods, introduced the elasticity coefficient, and explored the response of ESV change to land-use change, based on the land use cover data from 1990 to 2020. The results showed that from 1990 to 2020, YRB ecosystem service value showed an overall increasing trend, mainly because the ecological construction project increased forest and grasslands in this region. In the past 30 years, spatial characteristics of ESV in YRB was relatively stable. The high-value areas were mainly distributed in the upper Yellow River Basin, while the low-value areas were mainly distributed in the lower Yellow River Basin, as the cold and hot spots were reduced. The ESV barycenter coordinates showed the direction of the transfer trajectory, which is first to southwest, northeast, and then to southwest. From 2000 to 2010, YRB land-use change had greater impact on ESV. Since 2010, the disturbance of ecosystem services by land-use change has decreased. Consequently, the elastic index of the upstream and Loess Plateau regions were significantly higher than that of other regions, and the impact of land-use change on ecosystem services was more obvious, due to improved large-scale ecological construction projects implementation. Conclusively, this study recommends the use of comprehensive spatial-temporal assessment of ESV for sustainable development and ecological protection in the YRB.
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http://dx.doi.org/10.1038/s41598-022-17464-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338978PMC
July 2022

DNA methylation-based epigenetic signatures predict somatic genomic alterations in gliomas.

Nat Commun 2022 Jul 29;13(1):4410. Epub 2022 Jul 29.

Department of Radiation Oncology, NYU Grossman School of Medicine, New York, NY, USA.

Molecular classification has improved diagnosis and treatment for patients with malignant gliomas. However, classification has relied on individual assays that are both costly and slow, leading to frequent delays in treatment. Here, we propose the use of DNA methylation, as an emerging clinical diagnostic platform, to classify gliomas based on major genomic alterations and provide insight into subtype characteristics. We show that using machine learning models, DNA methylation signatures can accurately predict somatic alterations and show improvement over existing classifiers. The established Unified Diagnostic Pipeline (UniD) we develop is rapid and cost-effective for genomic alterations and gene expression subtypes diagnostic at early clinical phase and improves over individual assays currently in clinical use. The significant relationship between genetic alteration and epigenetic signature indicates broad applicability of our approach to other malignancies.
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http://dx.doi.org/10.1038/s41467-022-31827-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338285PMC
July 2022

Sexual Behavior Transition and Acute and Early HIV Infection Among Men Who Have Sex With Men: Evidence from an Open Cohort in China, 2011-2019.

Arch Sex Behav 2022 Jul 29. Epub 2022 Jul 29.

Epidemiology and Biostatistics Institute, School of Public Health, Tianjin Medical University, No. 22 Qixiangtai Road, Heping District, Tianjin, 300070, People's Republic of China.

The present study examined how sexual behaviors transit over time among men who have sex with men (MSM) and whether these transitions were associated with acute and early HIV (AEH) infection. An open cohort study was conducted using snowball sampling to recruit MSM via physical venues and Internet venues from 2011 to 2019, in China. Cox models were used to estimate the correlates of risk factors and AEH infection. We used Latent Transition Analysis (LTA) to describe behavioral profile and explore latent behavioral "trans-phenotypes," then examined the effect between different "trans-phenotypes" and AEH infection risk and explored the effect of characteristics as possible predictors of sexual behavior transition. Of 6502 MSM, a total of 1974 individuals with negative human immunodeficiency virus (HIV) tests at baseline and at least 2 visit records were included in the final analysis from 2011 to 2019. During an average 1.84 years of follow-up, 64 AEH infections were documented. In the multivariable Cox model, the association between condom use in last anal sex (HR: 0.095, 0.038-0.205), fewer sexual partners (HR: 0.375, 0.205-0.712), low frequency of condom use (HR: 3.592, 1.186-11.272), and AEH acquisition were found. The percentage of MSM with "maintain/develop safety-profile," "consistent risky-profile," and "safety-profile to risky-profile" were 52.48, 40.17, and 7.35%, respectively. Compared with "maintain\develop safety-profile," "consistent or develop risky-profile" had an increasing likelihood of AEH infection. Meanwhile, male sex workers (MSWs) or older MSM were more likely to consist or transit in "risky-profile." Having middle education is a risk factor to transit in "risky-profile" for MSM with "safety-profile" at baseline. In addition, MSM who accept health services in the past year engaged in higher proportion of developing safety-profile. Approximately half of MSM maintain or develop risky behavior with the increasing likelihood of AEH acquisition in China, which suggested that targeted and intensive interventions should be prioritized to maintain safety-profile. Clinical Trial Number: ChiCTR2000039500.
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http://dx.doi.org/10.1007/s10508-022-02320-9DOI Listing
July 2022

The LIM Protein AJUBA is a Potential Oncogenic Target and Prognostic Marker in Human Cancer Pan-Cancer Analysis.

Front Cell Dev Biol 2022 11;10:921897. Epub 2022 Jul 11.

School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, China.

The LIM (Lin-11, Isl1, MEC-3) domain protein AJUBA is involved in multiple biological functions, and its aberrant expression is related to the occurrence and progression of various cancers. However, there are no analytical studies on AJUBA in pan-cancer. We performed a comprehensive pan-cancer analysis and explored the potential oncogenic roles of AJUBA, including gene expression, genetic mutation, protein phosphorylation, clinical diagnostic biomarker, prognosis, and AJUBA-related immune infiltration based on The Cancer Genome Atlas and Genotype-Tissue Expression databases. The results revealed that the expression of AJUBA highly correlated with poor clinical outcomes in patients with different types of cancer. Meanwhile, AJUBA expression was positively correlated with cancer-associated fibroblasts in many human cancers, such as breast invasive carcinoma, colon adenocarcinoma, brain lower-grade glioma, lung adenocarcinoma (LUAD), and ovarian serous cystadenocarcinoma (OV). Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that AJUBA is mainly involved in protein serine/threonine kinase activity, cell-cell junction, covalent chromatin modification, and Hippo signaling pathway. The pan-cancer study reveals the oncogenic roles of AJUBA and provides a comprehensive understanding of the molecular biological genetic information of AJUBA in various tumors.
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http://dx.doi.org/10.3389/fcell.2022.921897DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309301PMC
July 2022

Topological Interaction among Molecular Cluster Assemblies Affords Tunable Viscoelasticity.

J Phys Chem Lett 2022 Aug 27;13(30):7009-7015. Epub 2022 Jul 27.

South China Advanced Institute for Soft Matter Science and Technology, Guangdong Provincial Key Laboratory of Functional and Intelligent Hybrid Materials and Devices, South China University of Technology, Guangzhou 510640, China.

In the assemblies of subnanoscale polyhedral oligomeric silsesquioxane, topological interaction makes the dominant contribution to their viscoelasticity with broad tunability. The assembly molecules are designed with dumbbell, triangular, and tetrahedral shapes, and they demonstrate an intrinsic glassy feature with neither long-range ordering nor supramolecular assembly formation in their bulk. Their viscoelasticity can be broadly tuned through the tailoring of molecular topologies, while the trimer and tetramer assemblies afford elastic moduli comparable to those of rubbers (∼0.5 MPa) even 80 K above their glass transition temperatures. Molecular dynamics studies reveal the topological constraints resulting from the topology-disrupted cooperative dynamics among the cluster assemblies, and this finally leads to the typical caging dynamics of the structural units and the elasticity of the bulk materials. Further broadband dielectric spectroscopy studies uncover the unique hierarchical relaxation dynamics, inspiring the strategy for the decoupling of mechanical strengths and toughness for the design of impact resistant materials.
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http://dx.doi.org/10.1021/acs.jpclett.2c01817DOI Listing
August 2022

Avian IRF1 and IRF7 Play Overlapping and Distinct Roles in Regulating IFN-Dependent and -Independent Antiviral Responses to Duck Tembusu Virus Infection.

Viruses 2022 07 9;14(7). Epub 2022 Jul 9.

College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

Avian interferon regulatory factors 1 and 7 (IRF1 and IRF7) play important roles in the host's innate immunity against viral infection. Our previous study revealed that duck tembusu virus (DTMUV) infection of chicken fibroblasts (DF1) and duck embryo fibroblasts (DEFs) induced the expression of a variety of IFN-stimulated genes (ISGs), including VIPERIN, IFIT5, CMPK2, IRF1, and IRF7. IRF1 was further shown to play a significant role in regulating the up-expression of VIPERIN, IFIT5, and CMPK2 and inhibiting DTMUV replication. In this study, we confirm, through overexpression and knockout approaches, that both IRF1 and IRF7 inhibit DTMUV replication, mainly via regulation of type I IFN expression, as well as the induction of IRF1, VIPERIN, IFIT5, CMPK2, and MX1. In addition, IRF1 directly promoted the expression of VIPERIN and CMPK2 in an IFN-independent manner when IRF7 and type I IFN signaling were undermined. We also found that non-structural protein 2B (NS2B) of DTMUV was able to inhibit the induction of IFN-β mRNA triggered by Newcastle disease virus (NDV) infection or poly(I:C) treatment, revealing a strategy employed by DTMUV to evade host's immunosurveillance. This study demonstrates that avian IRF7 and IRF1 play distinct roles in the regulation of type I IFN response during DTMUV infection.
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http://dx.doi.org/10.3390/v14071506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315619PMC
July 2022

Calibration of Radar RCS Measurement Errors by Observing the Luneburg Lens Onboard the LEO Satellite.

Sensors (Basel) 2022 Jul 20;22(14). Epub 2022 Jul 20.

State Key Laboratory of Astronautic Dynamics, Xi'an Satellite Control Center, Xi'an 710043, China.

Accurate radar RCS measurements are critical to the feature recognition of spatial targets. A calibration method for radar RCS measurement errors is proposed for the first time in the context of special target tracking by observing the Luneburg Lens onboard the LEO satellite. The Luneburg Lens has favorable RCS scattering properties for the radar microwave. Thus, the laboratory RCS measurements of the Luneburg Lens, with some fixed incident frequency and with different incident orientations for the radar microwave, will be implemented in order to build a database. The incident orientation for the radar microwave in the satellite body frame will be calculated by taking advantage of the precise orbit parameters, with errors only at the magnitude of several centimeters and within the actual satellite attitude parameters. According to the incident orientation, the referenced RCS measurements can be effectively obtained by the bilinear interpolation in the database. The errors of actual RCS measurements can thus be calibrated by comparing the referenced and the actual RCS measurements. In the RCS measurement experiment, which lasts less than 400 s, the actual RCS measurement errors of the Luneburg Lens are nearly less than 0 dBsm, which indicates that the RCS measurement errors of the spatial targets can be effectively calculated by the proposed calibration method. After the elaborated calibration, the RCS measurements of the spatial targets can be accurately obtained by radar tracking.
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http://dx.doi.org/10.3390/s22145421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325169PMC
July 2022

An Analytical Model for Cure-Induced Deformation of Composite Laminates.

Polymers (Basel) 2022 Jul 17;14(14). Epub 2022 Jul 17.

School of Mechanics and Aerospace Engineering, Southwest Jiaotong University, Chengdu 610031, China.

Curing deformation prediction plays an important role in guiding the tools, curing process design, etc. Analytical methods can provide a rapid prediction and in-depth understanding of the curing deformation mechanism. In this paper, an analytical model is presented to study the cure-induced deformation of composite laminates. Based on the classical laminate theory, the thermal stress and deformation of composites during the curing process are calculated by considering the evolution of the mechanical properties of resin. Additionally, the coupling stiffness of the laminate is taken into consideration in the analytical model. An interface layer between the tool and the part is developed to simulate the variation of the tool-part interaction with the degree of resin cure. The maximum curing deformations and deformation profiles of different lay-up composite parts predicted by the proposed model are compared with the results of the finite element method and previous literature reports. Then, a comprehensive parametric study is carried out to investigate the influence of curing cycle, geometry, tool thermal expansion, and resin characteristics on the curing deformation of composite parts. The results reveal that geometry has a significant influence on the curing deformation of composite parts, but for dimensionally determined parts, curing deformation is mainly attributable to their own anisotropy in macro and micro aspects, as well as the stretching effect of the tool on the part. The percentage contribution of different factors to curing deformation composites with different lay-ups and geometries is also discussed.
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http://dx.doi.org/10.3390/polym14142903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318655PMC
July 2022

An Analytical Model for Cure-Induced Deformation of Composite Laminates.

Polymers (Basel) 2022 Jul 17;14(14). Epub 2022 Jul 17.

School of Mechanics and Aerospace Engineering, Southwest Jiaotong University, Chengdu 610031, China.

Curing deformation prediction plays an important role in guiding the tools, curing process design, etc. Analytical methods can provide a rapid prediction and in-depth understanding of the curing deformation mechanism. In this paper, an analytical model is presented to study the cure-induced deformation of composite laminates. Based on the classical laminate theory, the thermal stress and deformation of composites during the curing process are calculated by considering the evolution of the mechanical properties of resin. Additionally, the coupling stiffness of the laminate is taken into consideration in the analytical model. An interface layer between the tool and the part is developed to simulate the variation of the tool-part interaction with the degree of resin cure. The maximum curing deformations and deformation profiles of different lay-up composite parts predicted by the proposed model are compared with the results of the finite element method and previous literature reports. Then, a comprehensive parametric study is carried out to investigate the influence of curing cycle, geometry, tool thermal expansion, and resin characteristics on the curing deformation of composite parts. The results reveal that geometry has a significant influence on the curing deformation of composite parts, but for dimensionally determined parts, curing deformation is mainly attributable to their own anisotropy in macro and micro aspects, as well as the stretching effect of the tool on the part. The percentage contribution of different factors to curing deformation composites with different lay-ups and geometries is also discussed.
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http://dx.doi.org/10.3390/polym14142903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318655PMC
July 2022

Interaction of Companilactobacillus crustorum MN047-derived bacteriocins with gut microbiota.

Food Chem 2022 Jul 19;396:133730. Epub 2022 Jul 19.

College of Food Science and Engineering, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China. Electronic address:

Companilactobacillus crustorum MN047-derived bacteriocins (CCDB) have inhibitory effects on the growth of pathogens. In this study, a pectin/zein beads delivery system was used to investigate the effects of CCDB on the dextran sulfate sodium-induced colitis in mice. The focus was given on aspects linked with the gut microbiota, intestinal epithelial barrier, oxidative stress, and inflammation. Results suggested that CCDB alleviated the pathological symptoms of colitis, including increased disease activity index and shortened colon length. CCDB strengthened the gut barrier by increasing goblet cells and promoting the expressions of MUC2 and tight junctions-related proteins. CCDB decreased oxidative mediators and increased antioxidant mediators in serum or colon tissue. Furthermore, CCDB reduced harmful bacteria and enriched beneficial bacteria, which further decreased serum LPS and increased fecal butyric acid. In addition, CCDB inhibited the overexpressions of proinflammatory cytokines, chemokines, and pathogens/LPS-activated TLR4/NF-κB pathway. Therefore, CCDB is a potential dietary supplement to relieve colitis.
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http://dx.doi.org/10.1016/j.foodchem.2022.133730DOI Listing
July 2022
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