Publications by authors named "Jie Wei"

752 Publications

High RSK4 expression constitutes a predictor of poor prognosis for patients with clear cell renal carcinoma.

Pathol Res Pract 2021 Oct 2;227:153642. Epub 2021 Oct 2.

State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital and School of Basic Medicine, Air Force Medical University, Xi'an, Shaan Xi Province, China. Electronic address:

Background: This research focuses on exploring RSK4 protein expression within Clear Cell Renal Cell Carcinoma (ccRCC), based on these investigations on level of expressions coupled with the relevance to clinicopathologic features and clinical outcomes.

Methods: The expression of RSK4 in 48 ccRCC and 20 hydronephrosis samples were under the detection of immunohistochemistry; besides, its relevance to the combination of clinicopathologic features with prognosis was committed in virtue of statistical approaches.

Results: The 48 ccRCC samples included 36 (75%, 36/48) positive for RSK4, while the positive rate in hydronephrosis samples were 5 (25%, 5/20). Statistical analysis showed that RSK4 in ccRCC samples express higher expression the hydronephrosis samples (P < 0.05). Furthermore, the expression of RSK4 in ccRCC samples weren't correlated with ages and genders (P > 0.05), while WHO/ISUP nucleolar grade harboured relevance to low survival rate (P = 0.018). Molecular researches demonstrated that over-expression of RSK4 was able to upgrade the proliferation capability of ccRCC cell lines.

Conclusions: According to the expression pattern and molecular systems featured RSK4 in ccRCCs, it performed the function of a latent independent prognostic factor performing the function of a newly built latent therapeutic aim oriented with the patients undergoing RCC. Moreover, the specific mechanism of action is expected to be revealed in the future research.
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http://dx.doi.org/10.1016/j.prp.2021.153642DOI Listing
October 2021

High-Throughput Detection of Multiple Contaminants Based on Portable Photoelectrochromic Sensor Chip.

Anal Chem 2021 Oct 13. Epub 2021 Oct 13.

School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, P. R. China.

With the increasing concerns about the environment and food safety, it is necessary to develop portable, low-cost, and high-throughput biosensors for the simultaneous detection of multiple contaminates. However, traditional photoelectrochemical (PEC) biosensors lack the ability of multiplexed assays due to the inherent mechanism limitation. Also, specialized instruments are necessary for most PEC biosensors. In this work, a portable high-throughput sensor chip has been successfully developed. By introducing electrochromic materials, the detection is based on color change instead of electric signals, which reduces the limitation of instruments. This designed sensor chip is composed of three parallel sensing channels fabricated by laser etching. Each channel is modified with TiO/3D--CN composites with excellent PEC activity and electrochromic material Prussian blue (PB). Under light illumination, photoinduced electrons generated by TiO/3D--CN are injected into PB, and blue PB is reduced to colorless Prussian white. Three organic contaminates, ochratoxin A, lincomycin, and edifenphos, can be simultaneously detected because the binding of these molecules with aptamers affects the electron transfer and the corresponding color changes. This portable and high-throughput sensor chip provides a convenient choice for multiplexed assays with good sensitivity and accuracy.
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http://dx.doi.org/10.1021/acs.analchem.1c03868DOI Listing
October 2021

Integrating Band Engineering and the Flexoelectric Effect Induced by a Composition Gradient for High Photocurrent Density in Bismuth Ferrite Films.

ACS Appl Mater Interfaces 2021 Oct 13. Epub 2021 Oct 13.

Institute for Superconducting and Electronic Materials (ISEM), University of Wollongong, Innovation Campus, Squires Way, North Wollongong, NSW 2500, Australia.

Photovoltaic energy as one of the important alternatives to traditional fossil fuels has always been a research hot spot in the field of renewable and clean solar energy. Very recently, the anomalous ferroelectric photovoltaic effect in multiferroic bismuth ferrite (BiFeO) has attracted much attention due to the above-bandgap photovoltage and switchable photocurrent. However, its photocurrent density mostly in the magnitudes of μA/cm resulted in a poor power conversion efficiency, which severely hampered its practical application as a photovoltaic device. In this case, a novel approach was designed to improve the photocurrent density of BiFeO through the cooperative effect of the gradient distribution of oxygen vacancies and consequently induced the flexoelectric effect realized in the (La, Co) gradient-doped BiFeO multilayers. Subsequent results and analysis indicated that the photocurrent density of the gradient-doped multilayer BiFeO sample was nearly 3 times as much as that of the conventional doped single-layer sample. Furthermore, a possible mechanism was proposed herein to demonstrate roles of band engineering and the flexoelectric effect on the photovoltaic performance of the gradient-doped BiFeO film.
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http://dx.doi.org/10.1021/acsami.1c13305DOI Listing
October 2021

Distinct roles of astroglia and neurons in synaptic plasticity and memory.

Mol Psychiatry 2021 Oct 12. Epub 2021 Oct 12.

State Key Laboratory of Organ Failure Research, Key Laboratory of Mental Health of the Ministry of Education, Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence, Guangdong Province Key Laboratory of Psychiatric Disorders, Collaborative Innovation Center for Brain Science, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China.

Long-term potentiation (LTP) in the hippocampus is the most studied form of synaptic plasticity. Temporal integration of synaptic inputs is essential in synaptic plasticity and is assumed to be achieved through Ca signaling in neurons and astroglia. However, whether these two cell types play different roles in LTP remain unknown. Here, we found that through the integration of synaptic inputs, astrocyte inositol triphosphate (IP) receptor type 2 (IPR2)-dependent Ca signaling was critical for late-phase LTP (L-LTP) but not early-phase LTP (E-LTP). Moreover, this process was mediated by astrocyte-derived brain-derived neurotrophic factor (BDNF). In contrast, neuron-derived BDNF was critical for both E-LTP and L-LTP. Importantly, the dynamic differences in BDNF secretion play a role in modulating distinct forms of LTP. Moreover, astrocyte- and neuron-derived BDNF exhibited different roles in memory. These observations enriched our knowledge of LTP and memory at the cellular level and implied distinct roles of astrocytes and neurons in information integration.
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http://dx.doi.org/10.1038/s41380-021-01332-6DOI Listing
October 2021

Letter Regarding Repeated-Measures ANOVA.

Authors:
Jie Wei Dalang Fang

J Clin Oncol 2021 Oct 12:JCO2101173. Epub 2021 Oct 12.

Jie Wei, MSc, Department of Hematology, People's Hospital of Baise, Baise, Guangxi, China, Department of Hematology, The Southwest Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China; and Dalang Fang, MSc, Department of Breast and Thyroid Surgery, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China.

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http://dx.doi.org/10.1200/JCO.21.01173DOI Listing
October 2021

Letter by Wei and Fang Regarding Article, "Blood Pressure Effects of Sodium Reduction: Dose-Response Meta-Analysis of Experimental Studies".

Authors:
Jie Wei Dalang Fang

Circulation 2021 Oct 11;144(15):e236. Epub 2021 Oct 11.

Department of Breast and Thyroid Surgery, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China (D.F.).

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http://dx.doi.org/10.1161/CIRCULATIONAHA.121.055647DOI Listing
October 2021

A human infection case with avian-origin H10N3 influenza virus.

Quant Imaging Med Surg 2021 Oct;11(10):4508-4510

Department of Radiology, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing, China.

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http://dx.doi.org/10.21037/qims-21-592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408795PMC
October 2021

Commentary: Chemokines in Prediabetes and Type 2 Diabetes: A Meta-Analysis.

Authors:
Jie Wei

Front Immunol 2021 16;12:729702. Epub 2021 Sep 16.

Department of Hematology, People's Hospital of Baise, Baise, China.

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http://dx.doi.org/10.3389/fimmu.2021.729702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8483574PMC
September 2021

Analgesic Effects of Two Types of Spinal Manipulation in Acute Lumbar Radiculopathy Model Rats.

Chin J Integr Med 2021 Sep 29. Epub 2021 Sep 29.

Spine Center of Manipulative Orthopedics, Air Force Medical Center, Beijing, 100142, China.

Objective: To compare the analgesic effects of two types of spinal manipulation (SM) in acute lumbar radiculopathy (ALR) model rats induced by self-transplantation of autologous nucleus pulposus (ANP), and clarify the therapeutic mechanism.

Methods: Totally 108 male Sprague-Dawley rats were randomly divided into 6 groups by a random number table (18 rats in each group), including a blank group with no interference, a sham operation group with a surgery by making a local soft tissue incision on the left side of L5-6 vertebral segment, a model group with ALR of L5 extraforaminal nerve by ANP self-transplantation without other interference, a sham manipulation (SMA) group with simulating physical rotation, as well as a mobilization (MOB) group with simulating low-velocity and variable-amplitude rotation and a manipulation (MAN) group with simulating high-velocity and low-amplitude rotation. The interventions in SMA, MOB, and MAN groups started 1 day after modeling followed by another 5 treatments at days 3, 5, 8, 10 and 12. Rats in the other 3 groups did not receive any special intervention. Behavioral pain tests of 50% mechanical pain withdrawal threshold (50% PWT) and paw withdrawal latency (PWL) were conducted 1 day before operation followed by another 10 tests on days 1-7, 10, 12 and 14. Immunohistochemical expression of nitric oxide synthase (NOS) was investigated on days 5 and 12 after operation.

Results: After 3 experimental SM interventions, 50% PWT and PWL were higher in the MAN group than the SMA group on days 6 and 7, and higher on days 10, 12 and 14 postoperatively (P<0.05 or P<0.01), while the same indices were significantly higher in the MOB group than MAN group on days 1-4 (P<0.05 or P<0.01). The expression of NOS was lower in the MAN and MOB groups than SMA group on day 12 postoperatively (P<0.01).

Conclusions: Both manipulation and mobilization produced better results than sham interference in relieving pain by reducing neuroinflammation possibly. At the early period, compared with manipulation, mobilization presented less sensitive response to pain until later visit. SM may inhibit the overexpression of NOS, thereby alleviating severe radiculopathy.
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http://dx.doi.org/10.1007/s11655-021-3276-yDOI Listing
September 2021

RGD-targeted redox responsive nano micelle: co-loading docetaxel and indocyanine green to treat the tumor.

Drug Deliv 2021 Dec;28(1):2024-2032

School of Pharmacy, Nanjing Tech University, Nanjing, China.

Cancer, also known as a malignant tumor, has developed into a type of disease with the highest fatality rate, seriously threatening the lives and health of people. Chemotherapy is one of the most important methods for the treatment of cancer. However, chemotherapy drugs have some problems, such as low solubility and lack of targeting, which severely limit their clinical applications. To solve these problems, we designed a block copolymer that has a disulfide bond response. The polymer uses RGD peptide (arginine-glycine-aspartic acid) as the active targeting group, PEG (polyethylene glycol) as the hydrophilic end, and PCL (polycaprolactone) as the hydrophobic end. Then we utilized the amphiphilic polymer as a carrier to simultaneously deliver DOC (docetaxel) and ICG (indocyanine green), to realize the combined application of chemotherapy and photothermal therapy. The antitumor efficacy and histology analysis showed that the DOC/ICG-loaded micelle exhibited higher antitumor activity. The drug delivery system improved the solubility of DOC and the stability of ICG, realized NIR-guided photothermal therapy, and achieved an ideal therapeutic effect.
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http://dx.doi.org/10.1080/10717544.2021.1977425DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477929PMC
December 2021

Letters to the Editor.

Authors:
Jie Wei

Menopause 2021 Sep 20. Epub 2021 Sep 20.

Department of Hematology, People's Hospital of Baise Zhuang Autonomous Region, Baise, China.

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http://dx.doi.org/10.1097/GME.0000000000001860DOI Listing
September 2021

A novel fibrinogen γ-chain frameshift mutation, p. Cys365Phefs*41, causing hypofibrinogenemia with bleeding phenotype in a Chinese family.

Ann Transl Med 2021 Aug;9(16):1308

Clinical Laboratory, The Affiliated Shunde Hospital of Jinan University, Foshan, China.

Background: Congenital hypofibrinogenemia is a rare bleeding disease that is classified as the quantitative deficient type. In the present study, investigated the relationship between the genotype and phenotype in a family with hypofibrinogenemia.

Methods: The proband was aware of a predisposition to bleeding. Functional analysis was performed for her all family members, including coagulation function tests, thrombus molecular markers, thromboelastography, scanning electron microscopy, DNA sequencing, and high-performance liquid chromatography-mass spectrometry (HPLC-MS). Pathogenicity analysis and protein modeling of mutant amino acids were also performed.

Results: A novel heterozygous mutation in c.1094delG was detected in FGG exon 8, which resulted in p. Cys365Phefs*41 (containing the signal peptide) in the proband and her mother, who showed a corresponding decrease in fibrinogen function and levels. Thromboelastography indicated that the strength of their blood clots decreased and they had an increased risk of bleeding. The proband fibrin network structure was looser than healthy controls, with large pores in the network, which increased the permeability of lytic enzymes. Results of HPLC-MS showed a lack of mutant peptide chain expression in their plasma, indicating that the family had congenital hypofibrinogenemia, with a clinical phenotype that is related to the degree of fibrinogen deficiency. The mutation truncated the γ-peptide chain and destroyed the functional structure of fibrinogen, including the γ352Cys-γ365Cys disulfide bond. The truncated peptide chains may also lead to nonsense-mediated decay.

Conclusions: The mutation induced a structural change at the carboxyl-terminal of the fibrinogen molecule, leading to fibrinogen secretion dysfunction.
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http://dx.doi.org/10.21037/atm-21-3207DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422118PMC
August 2021

N6-methyladenosine (m6A) regulatory gene divides hepatocellular carcinoma into three subtypes.

J Gastrointest Oncol 2021 Aug;12(4):1860-1872

Department of Hepatobiliary Surgery, the First Affiliated Hospital of Guangxi Medical University, Nannning, China.

Background: The N6-methyladenosine (m6A) plays an important role in epigenetic modification and tumor progression, but the modulations of m6A in hepatocellular carcinoma (HCC) have not been determined while the relationship between m6A regulation and immune cell infiltration remains unclear.

Methods: This study investigated the modification patterns of m6A by analyzing HCC samples from The Cancer Genome Atlas (TCGA) database and the Gene Expression Omnibus (GEO) dataset, and performed molecular typing based on the characteristics of immune cell infiltration. The m6Ascore was also constructed to quantify m6A modifications and predict the immunotherapy response and prognosis of HCC patients.

Results: Of the 364 samples, 31 (8.52%) were genetically altered in the m6A regulatory gene, with the highest frequency of mutations in , , and . Three distinct molecular subtypes of m6A were identified in 590 HCC samples, which were associated with different immune cell infiltrates: immunodepletion type, immune activation type, and immune immunity type. According to the construction of the m6Ascore system in the m6A genotype, HCC patients could be divided into high and low groups. The m6A modified pattern, characterized by immune immunity and immune failure, showed a lower score and a better prognosis. However, the immune-activated type of m6A had a higher score and a poorer prognosis. Further analysis showed that the m6Ascore was correlated with tumor mutation burden (TMB), and the higher the TMB, the worse the prognosis. m6Ascore was also correlated with the expression of cytotoxic T-lymphocyte-associated protein 4 (CTAL-4), and the higher the score, the higher the expression of HCC in patients.

Conclusions: HCC has a unique m6A modification pattern, and 3 different m6A subtypes help to classify HCC, provide knowledge of drug regimens for immunotherapy, and can be used to predict treatment response and prognosis.
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http://dx.doi.org/10.21037/jgo-21-378DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421920PMC
August 2021

Controlling the ligands of CdZnTe quantum dots to design a super simple ratiometric fluorescence nanosensor for silver ion detection.

Analyst 2021 Sep 13;146(18):5747-5755. Epub 2021 Sep 13.

School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.

A super simple ratiometric fluorescence nanosensor has been fabricated by controlling the ligands of CdZnTe quantum dots (QDs), allowing the sensitive and visual detection of silver ions (Ag). The green-emitting L-cysteine-protected CdZnTe QDs (Lcys-CdZnTe QDs) had a specific response to Ag and were used as the reporting probe, while the red-emitting -acetyl-L-cysteine-protected CdZnTe QDs (NAC-CdZnTe QDs) showed no obvious response to all tested metal ions and were selected as the reference probe. Simply mixing them without any encapsulated synthesis ultimately produced a time-saving, low-cost detection method, allowing the sensitive and visual detection of Ag in samples. The proposed nanosensor exhibited a linear range of 0.5-4.0 μM along with a detection limit of 0.17 μM, and has been successfully applied in real tap water and lake water samples. This nanosensor also showed obvious color changes in the detection process and has potential in visual semi-quantitative detection. Our approach may provide a general and feasible strategy for designing ratiometric fluorescence nanosensors, which will attract a wide range of interest in sensing-related fields.
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http://dx.doi.org/10.1039/d1an01200bDOI Listing
September 2021

Tantalum oxide submicro-particles into microporous coating on polyimide possessing antibacterial property and inducing cellular response for orthopedic application.

J Mech Behav Biomed Mater 2021 Aug 26;124:104800. Epub 2021 Aug 26.

Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, 200237, China. Electronic address:

Surface characteristics of the biomaterials have significant effects on response of osteoblast and formation of new bone tissue. In this study, to improve the bio-performance of polyimide (PI) as an implantable material for bone substitute, concentrated sulfuric acid suspension with tantalum (V) oxide (vTO) submicro-particles of 10w% (PIST10) and 15w% (PIST15) was utilized to modify PI surface. After sulfonation, microporous coatings including vTO particles were created on PI (PIST10 and PIST15) while microporous coating without vTO particles was also created on PI (PIS). Results showed that surface roughness, hydrophilicity and protein adsorption of PIST15 was remarkably higher than PIST10 and PIS. Furthermore, after soaking into simulated body fluid (SBF), no apatite mineralization on PIS was found, while PIST15 with high vTO content exhibited better apatite mineralization compared with PIST10. Moreover, PIS showed low antibacterial property, while PIST15 with high vTO content revealed better antibacterial property compared with PIST10. In addition, cellular response (such as adhesion, proliferation and alkaline phosphatase activity) of bone marrow stromal cells (BMSC) of rat to PIST15 was higher than PIST10 and PIS. In conclusion, the microporous coating of PIST15 including vTO submicro-particles possessed good antibacterial property and bioactivity, which significantly promoted the responses of BMSC. Therefore, PIST15 has potential application prospects for bone substitute.
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http://dx.doi.org/10.1016/j.jmbbm.2021.104800DOI Listing
August 2021

Novel Anti-Interference Strategy for a Self-Powered Sensor: Mediator-Free and Biospecific Photocathode Interface.

Anal Chem 2021 09 10;93(37):12690-12697. Epub 2021 Sep 10.

Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.

As a new electrochemical sensing concept, a self-powered sensor shows a good application prospect in the field of analysis. However, it is still a great challenge to improve the anti-interference capability of sensors through reasonable design. In this study, we investigated the difference between the single photoanode and photocathode self-powered sensor and combined the advantages of these two aspects to fabricate a mediator-free self-powered aptasensor based on the dual-photoelectrode system, which combined the biological events from the photocathode. The biological events occurred at the photocathode could avoid the interference caused by the generated hole oxidation of reducing small molecules in the real sample on the photoanode surface, which was helpful to enhance the anti-interference capability of the sensor. Moreover, due to the sufficient Fermi level differentiation between two photoelectrodes, the redox mediator was not necessary. This could avoid the redox reaction caused by the introduction of extra electron donors or electron acceptors occurring before the photoelectrical behavior, thus improving the accuracy of the sensor. According to the influence of the generated biological conjugate on the external circuit, electron transmission between interfaces, and the obstruction of visible light irradiation, the sensitive and accurate detection of the analytical model was achieved. This work provided a proof-of-concept for the establishment of a mediator-free dual-photoelectrode self-powered sensing platform with high sensitivity and strong anti-interference performance.
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http://dx.doi.org/10.1021/acs.analchem.1c02555DOI Listing
September 2021

TOPK: A new predictor of the therapeutic response to neoadjuvant chemotherapy and prognosis in triple-negative breast cancer.

Pathol Res Pract 2021 Oct 28;226:153603. Epub 2021 Aug 28.

State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital and School of Basic Medicine, Air Force Medical University, Xi'an, China. Electronic address:

Background: Triple-negative breast cancer (TNBC) has a high probability of relapse and poor overall survival. Neoadjuvant chemotherapy (NACT) is currently a routine treatment strategy for TNBC, but some patients do not respond well. T-LAK cell-originated protein kinase (TOPK) is highly expressed in breast cancer cells and contributes to cancer cell proliferation. The present study aimed to investigate the correlation of TOPK expression with NACT treatment response and prognosis in TNBC.

Methods: We collected 66 pairs of TNBC samples before and after NACT with docetaxel+ epirubicin+ cyclophosphamide (TEC). The Miller-Payne (MP) system was used to assess the therapeutic response to NACT in TNBC patients.

Results: Immunohistochemistry analysis showed that TNBC patients with high TOPK expression before NACT had a poor treatment response and a poor prognosis. The expression of TOPK after NACT was significantly higher than that before NACT in patients with MP grade 1-3. In contrast, patients with MP grade 4-5 had significantly lower TOPK expression after NACT than before NACT, and the expression change in Ki-67 in patients with MP grade 4-5 exhibited the same trend. Survival analysis revealed that patients with TNBC accompanied by elevated TOPK expression before NACT had a worse prognosis than those with lower TOPK expression.

Conclusion: TOPK may be a novel predictor for the therapeutic response to NACT and prognosis for patients with TNBC.
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http://dx.doi.org/10.1016/j.prp.2021.153603DOI Listing
October 2021

The Dual Role of Bridging Phenylene in an Extended Bipyridine System for High-Voltage and Stable Two-Electron Storage in Redox Flow Batteries.

ACS Appl Mater Interfaces 2021 Sep 9;13(37):44174-44183. Epub 2021 Sep 9.

MOE Key Laboratory of Mesoscopic Chemistry, MOE Key Laboratory of High Performance Polymer Materials and Technology, Jiangsu Key Laboratory of Advanced Organic Materials, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Aqueous organic redox flow batteries (AORFBs) are regarded as a promising solution for grid-scale and sustainable energy storage, but some long-standing problems such as low energy density and cycling stability should be resolved. Herein, a highly soluble bipyridine modified with a bridging phenylene group and two quaternary ammonium terminals, namely, , was synthesized and used as an ultralow-potential and two-electron storage anolyte for AORFBs. The phenylene group, which is linked but not coplanar with the two pyridinium redox centers, can thus prevent their communication and result in an exceptionally low redox potential (-0.77 V vs standard hydrogen electrode, 2e). Moreover, the introduction of a phenylene group can warrant a certain degree of large π-conjugation effects and mitigate the intramolecular Coulombic repulsion between the two positively charged pyridinium centers, thus helping to enhance the electrochemical stability. When paired with 4-trimethylammonium-TEMPO as the catholyte, enabled an exceptionally high cell voltage up to 1.71 V. The AORFB delivers outstanding battery performances, specifically, ∼89% energy efficiency, ∼100% Coulombic efficiency, and ∼99.94% capacity retention per cycle during a long-term cycling process. The two overlapped single-electron reductions of from the initial cationic form to the monoradical form and then to the quinoid form during the charging process were clearly verified by a series of spectroscopic techniques, including no-deuterium nuclear magnetic resonance and electron paramagnetic resonance. This work presents a significant improvement for the construction of high-voltage AORFBs by virtue of the designability, diversity, and tunability of multiredox organic molecules.
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http://dx.doi.org/10.1021/acsami.1c09019DOI Listing
September 2021

The active fragments of ghrelin cross the blood-brain barrier and enter the brain to produce antinociceptive effects after systemic administration.

Can J Physiol Pharmacol 2021 Oct 7;99(10):1057-1068. Epub 2021 Sep 7.

Department of Physiology, Medical College of Nanchang University, Bayi Road 461, Nanchang, Jiangxi, 330006, China.

G (1-5)-NH, G (1-7)-NH, and G (1-9) are the active fragments of ghrelin. The aim of this study was to investigate the antinociceptive effects, their ability to cross the blood-brain barrier, and the receptor mechanism(s) of these fragments using the tail withdrawal test in male Kunming mice. The antinociceptive effects of these fragments (2, 6, 20, and 60 nmol/mouse) were tested at 5, 10, 20, 30, 40, 50, and 60 min after intravenous (i.v.) injection. These fragments induced dose- and time-related antinociceptive effects relative to saline. Using the near infrared fluorescence imaging experiments, our results showed that these fragments could cross the brain-blood barrier and enter the brain. The antinociceptive effects of these fragments were completely antagonized by naloxone (intracerebroventricular, i.c.v.); however, naloxone methiodide (intraperitoneal, i.p.), which is the peripheral restricted opioid receptor antagonist, did not antagonize these antinociceptive effects. Furthermore, the GHS-R1α antagonist [D-Lys]-GHRP-6 (i.c.v.) completely antagonized these antinociceptive effects, too. These results suggested that these fragments induced antinociceptive effects through central opioid receptors and GHS-R1α. In conclusion, our studies indicated that these active fragments of ghrelin could cross the brain-blood barrier and enter the brain and induce antinociceptive effects through central opioid receptors and GHS-R1α after intravenous injection.
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http://dx.doi.org/10.1139/cjpp-2020-0668DOI Listing
October 2021

The Role of Gut Microbiota in Tumor Immunotherapy.

J Immunol Res 2021 26;2021:5061570. Epub 2021 Aug 26.

Department of Critical Care Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.

Tumor immunotherapy is the fourth therapy after surgery, chemotherapy, and radiotherapy. It has made great breakthroughs in the treatment of some epithelial tumors and hematological tumors. However, its adverse reactions are common or even more serious, and the response rate in some solid tumors is not satisfactory. With the maturity of genomics and metabolomics technologies, the effect of intestinal microbiota in tumor development and treatment has gradually been recognized. The microbiota may affect tumor immunity by regulating the host immune system and tumor microenvironment. Some bacteria help fight tumors by activating immunity, while some bacteria mediate immunosuppression to help cancer cells escape from the immune system. More and more studies have revealed that the effects and complications of tumor immunotherapy are related to the composition of the gut microbiota. The composition of the intestinal microbiota that is sensitive to treatment or prone to adverse reactions has certain characteristics. These characteristics may be used as biomarkers to predict the prognosis of immunotherapy and may also be developed as "immune potentiators" to assist immunotherapy. Some clinical and preclinical studies have proved that microbial intervention, including microbial transplantation, can improve the sensitivity of immunotherapy or reduce adverse reactions to a certain extent. With the development of gene editing technology and nanotechnology, the design and development of engineered bacteria that contribute to immunotherapy has become a new research hotspot. Based on the relationship between the intestinal microbiota and immunotherapy, the correct mining of microbial information and the development of reasonable and feasible microbial intervention methods are expected to optimize tumor immunotherapy to a large extent and bring new breakthroughs in tumor treatment.
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http://dx.doi.org/10.1155/2021/5061570DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413023PMC
August 2021

Evolution of aerosol chemistry in Beijing under strong influence of anthropogenic pollutants: Composition, sources, and secondary formation of fine particulate nitrated aromatic compounds.

Environ Res 2021 Sep 1;204(Pt A):111982. Epub 2021 Sep 1.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China. Electronic address:

Nitrated aromatic compounds (NACs) constitute a key segment of brown carbon (BrC), thereby contributing to the light-absorbing characteristics of aerosols in the atmosphere. However, until recently, there is a scarcity of research on their generation in the urban environment. The current study is based upon an extensive field study of NACs from fine particle samples obtained at an urban location in Beijing in the spring and summer of 2017, which was characterized by both high anthropogenic volatile organic compounds (VOCs) and high-NO dominated conditions. The mean total concentration of the nine NACs was 8.58 ng m in spring and 8.54 ng m in summer. In the spring, the most abundant NACs were 4-nitrophenol (33.7%) and 4-nitrocatechol (19.3%), while in the summer, the most abundant NACs were 4-nitroguaiacol (34.9%) and 2, 4-dinitrophenol (23%). Atmospheric NACs were primarily produced from coal combustion (52%) and biomass burning (32%) in spring, and originated from the secondary formation (37%) and traffic (35%) in summer. NO could promote the formation of NACs with a significant effect on their compositions, especially for nitrophenols and nitrocatechols. It can also affect the formation of nitrated aerosols and their existing form. Inorganic nitrates were increased to conversion in the daytime when NO concentrations were higher than 30 ppb, but the corresponding oxidation products shifted to primarily organic ones at night. The transition was VOC-sensitive regimes for NAC formation, and nitration of toluene was a more important pathway during the campaign in Beijing.
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http://dx.doi.org/10.1016/j.envres.2021.111982DOI Listing
September 2021

The Regulation of Prototype Foamy Virus 5'Long Terminal Repeats and Internal Promoter by Endogenous Transcription Factors.

Intervirology 2021 Aug 26:1-12. Epub 2021 Aug 26.

College of Life Sciences, Shaanxi Normal University, Xi'an, China.

Background: For foamy virus, the transactivator of spumaretrovirus (Tas) could bind directly to target DNA sequences termed as Tas responsive elements and trigger the viral internal promoter (IP) and long terminal repeat (LTR) promoters. The cellular endogenous factors also play an important role in viral gene expressions. We hypothesized that except the viral transcription factor Tas, the cellular endogenous factors also affect the viral gene expression.

Methods: The full length of the prototype foamy virus (PFV) genome (U21247) was used to predict the potential binding sites of the transcription factors by online software JASPAR (http://jaspar.genereg.net) and Softberry (http://linux1.softberry.com/berry.phtml?topic=index&group=programs&subgroup=promoter). The Dual-Luciferase® Reporter Assay System (Promega, USA) was used to confirm the relative luciferase activities of the test groups. The different representative activating agents or inhibitors of each canonical signal pathway were used to identify the impact of these pathways on PFV 5'LTR and IP promoters.

Results: The results showed different cellular endogenous factors might have respective effects on PFV 5'LTR and IP. It is worth mentioning that activator protein-1 and BCL2-associated athanogene 3, 2 kinds of vital proteins associated with NF-κB and PKC pathways, could activate the basal activity of 5'LTR and IP promoters but inhibit the Tas-regulated activity of both promoters. Furthermore, PFV Tas was identified to trigger the transcription of the NF-κB promoter.

Conclusion: NF-κB had a negative effect on PFV 5'LTR and IP promoter activities, the PKC pathway might upregulate 5'LTR and IP promoter activities, and the JNK and NF-AT signal pathway could increase the Tas-regulated promoter activity of PFV 5'LTR. This study sheds light on the interaction between PFV and the host cell and may help utilize the viral promoters in retroviral vectors designed for gene transfer experiments.
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http://dx.doi.org/10.1159/000517539DOI Listing
August 2021

Screening a novel signature and predicting the immune landscape of metastatic osteosarcoma in children via immune-related lncRNAs.

Transl Pediatr 2021 Jul;10(7):1851-1866

Department of Traumatology, Baise People's Hospital, Baise, China.

Background: The immune microenvironment plays an essential role in osteosarcoma (OSs); however, differences in immune-related long non-coding ribonucleic acids (irlncRNAs) in children with localized OSs and metastatic OSs have not yet been investigated.

Methods: The clinical data and the transcriptome of OSs were obtained from the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database, and the immune-related genes were derived from the imported dataset. The correlations between immune-related genes and lncRNAs were examined. Next, the differential expressions of the irlncRNA pairs (IRLPs) in localized OSs and distant metastatic OSs were analyzed, and a prognostic model was constructed based on the significant differentially expressed IRLPs. We also analyzed the association between the IRLPs' signature risk score and the infiltration of the immune cells. Finally, we investigated the correlation between risk score and drug resistance.

Results: Thirty upregulated and 22 downregulated lncRNAs were identified in the localized and metastatic OSs samples. Univariate and multivariate cox regression analyses were undertaken to select 6 lncRNA pairs to establish the prognostic signature, the model was valuable in predicting OSs prognosis. Further, the expression of the finally selected irlncRNAs indicated that VPS9D1-AS1 (P=0.031), AP003086.2 (P=0.041), AL031847.1 (P=0.008), AL020997.3 (P=0.020), AC011444.1 (P=0.025), and AC006449.2 (P=0.003) were significantly upregulated in metastasis patients, but USP27X-AS1 (P=0.046), AL008721.2 (P=0.005), AC002091.1 (P=0.033), and AL118558.4 (P=0.049) were significantly overexpressed in localized patients. The overexpression of AC002091.1 (P=0.038) and AL118558.4 (P=0.004) resulted in better overall survival, but the upregulation of AC011444.1 (P=0.045), AL031847.1 (P=0.020), VPS9D1-AS1 (P=0.039), and AC006449.2 (0.006) led to a poor outcome. Differences in immune cell infiltration indicated that metastatic patients and localized have significant difference of 4 (CD4) T cells (P=0.006), monocytes (P=0.029), activated mast cells (P=0.018), and neutrophils (P=0.026), and a high abundance of activated dendritic cells (P=0.010) and activated mast cells (P=0.049) resulted in poor prognosis. Patients in the high-risk-score group were resistant to axitinib, but sensitive to dasatinib, bortezomib, and cisplatin.

Conclusions: In the present study, IRLPs were used to construct a novel and practical model for predicting the prognosis of localized and metastatic OSs in children.
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http://dx.doi.org/10.21037/tp-21-226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349967PMC
July 2021

Novel dual-emissive fluorescent immunoassay for synchronous monitoring of okadaic acid and saxitoxin in shellfish.

Food Chem 2022 Jan 13;368:130856. Epub 2021 Aug 13.

Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8520, Japan.

In this work, we present a novel dual-emissive fluoroimmunoassay for synchronous monitoring of okadaic acid (OA) and saxitoxin (STX) using multicolor fluorescent labels composed of sulfur, phosphorous co-doped graphene quantum dots (S, P-GQDs), and ovalbumin (OVA)-coated gold nanoparticles (OVA-AuNPs). The novel OVA-AuNPs were prepared by the reduction of chloroauric acid under alkaline conditions using OVA as a reducing agent. Both S, P-GQDs and OVA-AuNPs exhibit bright fluorescence, more importantly, a large emission wavelength difference (Δλ = 156 nm) under an excitation of 400 nm and relatively independent fluorescence behavior, which are essential to realizing the dual-signal marks in a directly mixing system. Using a competitive fluorescence-linked immunosorbent assay (cFLISA) format, the dual-emissive cFLISA was successfully utilized to measure OA and STX contents in Alectryonella plicatula (commonly named as fingerprint oyster) and the detection results were in good agreement with the commercial enzyme-linked immunosorbent assay (ELISA) kits.
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http://dx.doi.org/10.1016/j.foodchem.2021.130856DOI Listing
January 2022

D-dimer level for ruling out peripherally inserted central catheter-associated upper extremity deep vein thrombosis and superficial vein thrombosis.

Nurs Open 2021 Aug 16. Epub 2021 Aug 16.

Institute for Rational and Safe Medication Practices, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China.

Aims: To examine the effectiveness of D-dimer values to be used as an independent diagnostic marker for excluding peripherally inserted central catheter-associated upper extremity deep vein thrombosis and superficial vein thrombosis.

Design: This was a retrospective case-cohort study.

Methods: Records were reviewed for 281 patients who underwent peripherally inserted central catheter insertion between 1 October 2017 and 1 October 2019. According to the modified Wells score after peripherally inserted central catheter insertion, the patients who had low vein thrombosis risk underwent a D-dimer test and colour Doppler ultrasound.

Results: Among 281 patients, 180 patients (64%, 95% CI: 58.2%-69.4%) had negative D-dimer results and 39 of 180 patients had vein thrombosis despite having a negative D-dimer result, resulting in a failure rate of 21.7% (95% CI: 16.3%-28.3%). The negative predictive value of peripherally inserted central catheter-associated vein thrombosis in the cancer group (80.0%, 95% CI: 73.2%-85.4%) was higher than that of the non-cancer group (60.0%, 95% CI: 35.7%-80.2%). The negative predictive value of peripherally inserted central catheter-associated deep venous thrombosis (84.9%, 95% CI: 78.7%-89.6%) was lower than that of the PICC-associated superficial venous thrombosis (91.0%, 95% CI: 85.4%-94.6%).

Conclusion: The D-dimer levels maybe should not be used as a diagnostic index to rule out peripherally inserted central catheter-associated upper extremity vein thrombosis.
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http://dx.doi.org/10.1002/nop2.998DOI Listing
August 2021

Mutant APC promotes tumor immune evasion via PD-L1 in colorectal cancer.

Oncogene 2021 Oct 12;40(41):5984-5992. Epub 2021 Aug 12.

Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, USA.

PD-L1 expression is elevated in various human cancers, including colorectal cancer. High levels of PD-L1 expressed on tumor epithelial cells are one of the potential mechanisms by which tumor cells become resistant to immune attack. However, PD-L1 regulation in tumor cells is not fully understood. Here we demonstrate that mutations in the adenomatous polyposis coli (APC) gene lead to colonic epithelial cell resistance to CD8 T cell cytotoxicity by induction of PD-L1 expression. Mechanistically, this occurs as a result of the β-catenin/TCF4 complex binding to the PD-L1 promoter, leading to increased transcription. Our findings not only reveal a novel mechanism by which APC mutations induce tumor immune evasion via an immune checkpoint pathway but also pave the way for developing β-catenin or TCF4 inhibitors as possible new options for immune checkpoint inhibition.
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http://dx.doi.org/10.1038/s41388-021-01972-6DOI Listing
October 2021

Identifying predictors of response to oral non-steroidal anti-inflammatory drugs and paracetamol in osteoarthritis: a hypothesis-driven protocol for an OA Trial Bank individual participant data meta-analysis.

BMJ Open 2021 08 11;11(8):e048652. Epub 2021 Aug 11.

Academic Rheumatology, Clinical Sciences Building, University of Nottingham, City Hospital, Nottingham, UK.

Introduction: Symptomatic treatments for osteoarthritis (OA) provide only small-to-moderate efficacy over placebo in randomised controlled trials (RCTs). Treatment guidelines therefore have emphasised the need to identify predictors of treatment response through subgroup and multiple regression analysis. Individual participant data (IPD) meta-analysis is recommended as an efficient approach for this purpose. To our knowledge, this has not been undertaken for oral non-steroidal anti-inflammatory drugs (NSAIDs), including paracetamol, in OA. In this IPD meta-analysis, we aim to identify RCTs with specific mechanistic features related to OA pain, such as joint inflammation. We hypothesise that NSAIDs may work better for participants with joint inflammation, whereas paracetamol may not.

Methods And Analysis: A comprehensive literature search will be conducted on the databases of Web of Science, Embase, Medline, CINAHL, AMED and the Cochrane Library from 1 January 1998 to 1 December 2020. All RCTs related to oral NSAIDs or paracetamol including placebo-controlled trials in people with OA that have evaluated pain-related peripheral risk factors (eg, clinically detected knee effusion, synovial hypertrophy or effusion on imaging, knee morning stiffness, elevated serum C-reactive protein (CRP) level) and/or central pain risk factors (eg, pain elsewhere, depression, anxiety, sleep disturbance) will be retrieved. The outcome will be change in pain from baseline. Change in function and patient global assessment will also be included as outcomes if available. Investigators of all eligible trials will be contacted for IPD. Multilevel regression models will be used to identify predictors for the specific (active-placebo) and the overall treatment effect (change from baseline in active group).

Ethics And Dissemination: No identifiable data will be included in this study and no formal ethics approval is required as no new data collection will be processed. Results of this hypothesis-driven IPD meta-analysis will be disseminated through conference presentations and publication in peer-reviewed journals.

Prospero Registration Number: CRD42020165098.
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http://dx.doi.org/10.1136/bmjopen-2021-048652DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359469PMC
August 2021

Letter regarding 'The global prevalence of apical periodontitis: A systematic review and meta-analysis'.

Authors:
Jie Wei Dalang Fang

Int Endod J 2021 09;54(9):1677

Department of Breast and Thyroid Surgery, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.

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http://dx.doi.org/10.1111/iej.13590DOI Listing
September 2021

Tough and Multifunctional Composite Film Actuators Based on Cellulose Nanofibers toward Smart Wearables.

ACS Appl Mater Interfaces 2021 Aug 9;13(32):38700-38711. Epub 2021 Aug 9.

Beijing Engineering Research Center of Cellulose and Its Derivatives, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, P. R. China.

Although humidity-responsive actuators serve as a promising candidate in smart wearables, artificial muscles, and biomimetic devices, most of them derived from synthetic polymers could not simultaneously achieve multifunctional properties. In this work, a cellulose nanofiber (CNF)-based film actuator with high mechanical properties, excellent Joule heating, and antibacterial capability is successfully constructed by integrating with TiCT (MXene) and tannic acid (TA) via a vacuum-assisted filtration approach. Owing to the unique nacrelike structure and strong hydrogen bonds, the tensile strength and toughness of the composite film could reach 275.4 MPa and 10.2 MJ·m, respectively. Importantly, the hydrophilic nature of CNFs and alterable interlayer spacing of MXene nanosheets endow the composite film with sensitive humidity response and extraordinary stability (1000 cycles). With the assistance of MXene nanosheets and TA, the composite film could not only present outstanding Joule heating but also possess remarkable antibacterial properties against both Gram-negative and Gram-positive . Benefiting from the above merits, the proof-of-concept smart garment is assembled by the as-prepared film and is capable of regulating humidity and temperature.
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http://dx.doi.org/10.1021/acsami.1c09653DOI Listing
August 2021

Clinical Significance of Decreased GPX1 Expression in Patients with Acute Myeloid Leukaemia (Non-M3).

J Coll Physicians Surg Pak 2021 Aug;31(8):941-946

Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Objective: To determine the expression levels of GPX1, SOCS5 and IL7 and their clinical significance in patients with acute myeloid leukaemia (AML).

Study Design: A case-control study.

Place And Duration Of Study: Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China, from January 2013 to November 2020.

Methodology: Based on the bioinformatics analysis, the expression levels of GPX1, SOCS5 and IL7 in the bone marrow of 64 AML patients (non-M3) and 32 healthy individuals were evaluated by real-time PCR. Correlation between GPX1 expression and the clinical characteristics, response to induced chemotherapy, and survival time of AML patients were analysed as the outcome measure.

Results: GPX1 was significantly downregulated in AML patients, which helped in distinguishing AML patients from normal controls. The area under the curve (AUC) of the receiver operator characteristic (ROC) was 0.741 (p <0.001). Additionally, GPX1 expression was correlated with gender (r = -0.250, p = 0.045), FAB classification (r = -0.332,  p = 0.004), and chemotherapy response (r = 0.366, p = 0.003). AML patients with high GPX1 expression levels had a lower rate of remission (p = 0.021) and poor long-term survival (p = 0.036) than those with low GPX1 expression levels.

Conclusion: Low GPX1 expression in AML patients may be closely associated with the pathogenesis and chemoresistance of AML. Key Words: Acute myeloid leukaemia, Clinical outcome, Gene expression, GPX1.
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http://dx.doi.org/10.29271/jcpsp.2021.08.941DOI Listing
August 2021
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