Publications by authors named "Jie Tang"

1,189 Publications

  • Page 1 of 1

Weekly versus triweekly cisplatin-based concurrent chemoradiotherapy for nasopharyngeal carcinoma: a systematic review and pooled analysis.

J Cancer 2021 28;12(20):6209-6215. Epub 2021 Aug 28.

Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Weekly and triweekly cisplatin-based concurrent chemoradiotherapy (CCRT) have been used in the treatment of nasopharyngeal carcinoma (NPC). This study aimed to compare the benefits and risks between the two treatments. We systematically searched electronic databases for prospective and retrospective clinical studies of NPC patients who received weekly compared with triweekly cisplatin-based CCRT. The primary endpoints comprised overall, failure-free, distant metastasis-free, and locoregional recurrence-free survivals (OS, FFS, DMFS, and LRFS). Secondary endpoints were toxicities. Six studies were included in the systematic review, of which four with 1515 NPC patients were eligible for further pooled analysis. There were no significant differences between weekly and triweekly groups in terms of 5-year OS (odds ratio [OR] 0.95, 95% confidence interval [CI] 0.51-1.79), FFS (OR 1.09, 95% CI 0.67-1.76), DMFS (OR 1.25, 95% CI 0.54-2.92), and LRFS (OR 0.83, 95% CI 0.55-1.25). For grade ≥ 3 toxicities, the weekly group had higher risks of anemia (risk ratio [RR] 2.96, 95% CI 1.12-7.81) and thrombocytopenia (RR 2.75, 95% CI 1.54-4.90), but a lower incidence of vomiting (RR 0.34, 95% CI 0.18-0.63) versus the triweekly group. Both weekly and triweekly schedules could be recommended to NPC patients during CCRT. Additionally, hematologic adverse events in weekly strategy and non-hematologic adverse events in triweekly strategy are of higher concern.
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http://dx.doi.org/10.7150/jca.62188DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425212PMC
August 2021

The mitogenome of common snipe, Linnaeus, 1758 and evolutionary implications for the family Scolopacidae.

Mitochondrial DNA B Resour 2021 9;6(10):2886-2889. Epub 2021 Sep 9.

Shaanxi Institute of Zoology, Xi'an, China.

The mitochondrial genome (mitogenome) of Linnaeus, 1758 was determined by the high-throughput data. The assembled mitogenome was 16,919 bp in length, with a 58.7% A + T content and GC skew of -0.3850. Among 13 PCGs, an unusual start codon (GTG) was identified for the gene, and incomplete stop codons (T-) were found in the , and genes. The function of a cytosine insertion at site 174 in the gene and its phylogenetic significance are worthy of further scrutiny. In the control region (), thirteen 15-bp simple sequence repeats were found in . Phylogenetic analysis indicated that was clustered at the basal position of the clade and that the monophyly of was also recovered. The mitogenome data of provides useful resources for further studying the evolution of Scolopacidae.
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http://dx.doi.org/10.1080/23802359.2021.1972870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8439222PMC
September 2021

Decreased nocturnal heart rate variability and potentially related brain regions in arteriosclerotic cerebral small vessel disease.

BMC Neurol 2021 Sep 16;21(1):361. Epub 2021 Sep 16.

Department of Neurology, North Huashan Hospital, Fudan University, No.108 Lu Xiang Road, Shanghai, 201900, China.

Background: To assess heart rate variability (HRV) among patients with arteriosclerotic cerebral small vessel disease (CSVD) by comparing with control subjects, and to determine whether HRV parameters were related to structural alterations in brain regions involved in autonomic regulation among CSVD patients.

Methods: We consecutively recruited subjects aged between 50 and 80 years who visited the Stroke Prevention Clinic of our hospital and have completed brain magnetic resonance imaging examination from September 1, 2018 to August 31, 2019. Polysomnography and synchronous analyses of HRV were then performed in all participants. Multivariable binary logistic regression was used to identify the relationship between HRV parameters and CSVD. Participants were invited to further undergo three-dimensional brain volume scan, and the voxel based morphometry (VBM) analysis was used to identify gray matter atrophy.

Results: Among 109 participants enrolled in this study, 63 were assigned to the arteriosclerotic CSVD group and 46 to the control group. Lower standard deviation of normal-to-normal intervals (SDNN, OR = 0.943, 95% CI 0.903 to 0.985, P = 0.009) and higher ratio of low to high frequency power (LF/HF, OR = 4.372, 95% CI 1.033 to 18.508, P = 0.045) during the sleep period were associated with CSVD, independent of traditional cerebrovascular risk factors and sleep disordered breathing. A number of 24 CSVD patients and 21 controls further underwent three-dimensional brain volume scan and VBM analysis. Based on VBM results, SDNN during the awake time (β = 0.544, 95% CI 0.211 to 0.877, P = 0.001) and the sleep period (β = 0.532, 95% CI 0.202 to 0.862, P = 0.001) were both positively related with gray matter volume within the right inferior frontal gyrus only among CSVD patients.

Conclusions: Decreased nocturnal HRV is associated with arteriosclerotic CSVD independent of traditional cerebrovascular risk factors and sleep disordered breathing. The structural atrophy of some brain regions associated with cardiac autonomic regulation sheds light on the potential relationship.

Trial Registration: Trial registration number: ChiCTR1800017902 . Date of registration: 20 Aug 2018.
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http://dx.doi.org/10.1186/s12883-021-02388-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447504PMC
September 2021

Extracellular Vesicles: Emerging Therapeutics in Cutaneous Lesions.

Int J Nanomedicine 2021 7;16:6183-6202. Epub 2021 Sep 7.

Department of Dermatology, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China.

Extracellular vesicles (EVs), as nanoscale membranous vesicles containing DNAs, RNAs, lipids and proteins, have emerged as promising diagnostic and therapeutic agents for skin diseases. Here, we summarize the basic physiology of the skin and the biological characteristic of EVs. Further, we describe the applications of EVs in the treatment of dermatological conditions such as skin infection, inflammatory skin diseases, skin repair and rejuvenation and skin cancer. In particular, plant-derived EVs and clinical trials are discussed. In addition, challenges and perspectives related to the preclinical and clinical applications of EVs are highlighted.
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http://dx.doi.org/10.2147/IJN.S322356DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434831PMC
September 2021

Single-cell exome sequencing reveals multiple subclones in metastatic colorectal carcinoma.

Genome Med 2021 Sep 10;13(1):148. Epub 2021 Sep 10.

National Frontier Center of Disease Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, No. 17, Section 3, Renmin South Road, Chengdu, 610041, Sichuan, China.

Background: Colorectal cancer (CRC) is a major cancer type whose mechanism of metastasis remains elusive.

Methods: In this study, we characterised the evolutionary pattern of metastatic CRC (mCRC) by analysing bulk and single-cell exome sequencing data of primary and metastatic tumours from 7 CRC patients with liver metastases. Here, 7 CRC patients were analysed by bulk whole-exome sequencing (WES); 4 of these were also analysed using single-cell sequencing.

Results: Despite low genomic divergence between paired primary and metastatic cancers in the bulk data, single-cell WES (scWES) data revealed rare mutations and defined two separate cell populations, indicative of the diverse evolutionary trajectories between primary and metastatic tumour cells. We further identified 24 metastatic cell-specific-mutated genes and validated their functions in cell migration capacity.

Conclusions: In summary, scWES revealed rare mutations that failed to be detected by bulk WES. These rare mutations better define the distinct genomic profiles of primary and metastatic tumour cell clones.
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http://dx.doi.org/10.1186/s13073-021-00962-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434739PMC
September 2021

Autophagy during drought: function, regulation, and potential application.

Plant J 2021 Sep 1. Epub 2021 Sep 1.

Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, 50011, USA.

Drought is a major challenge for agricultural production since it causes substantial yield reduction and economic loss. Autophagy is a subcellular degradation and recycling pathway that functions in plant development and responses to many stresses, including drought. In this review, we summarize the current understanding of the function of autophagy and how autophagy is upregulated during drought stress. Autophagy helps plants to survive drought stress, and the mechanistic basis for this is beginning to be elucidated. Autophagy can selectively degrade aquaporins to adjust water permeability, and also degrades excess heme and damaged proteins to reduce their toxicity. In addition, autophagy can degrade regulators or components of hormone signaling pathways to promote stress responses. During drought recovery, autophagy degrades drought-induced proteins to reset the cell status. Autophagy is activated by multiple mechanisms during drought stress. Several transcription factors are induced by drought to upregulate autophagy-related gene expression, and autophagy is also regulated post-translationally through protein modification and stability. Based on these observations, manipulation of autophagy activity may be a promising approach for conferring drought tolerance in plants.
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http://dx.doi.org/10.1111/tpj.15481DOI Listing
September 2021

Anatomical Study of Ethmoidal Foramina and Optic Canal in Endoscopic Trans-Nasal Approach to Anterior Skull Base.

J Craniofac Surg 2021 Aug 27. Epub 2021 Aug 27.

Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing Department of Neurosurgery, People's Hospital of Rizhao, Rizhao, China.

Background: Surgery to anterior skull base is challenging since complex structures are involved. Injury of olfactory fila and optic nerve may result in postoperative complications. In our study, the authors aim to use computer topographic image to provide a comprehensive anatomical information of anterior skull base and set up a new classification of optic canal (OC) in ethmoid sinus by the degree of bony defect, so as to help surgeons in endoscopic transnasal approach to anterior skull base.

Method: Computer topographic angiography images of 112 individuals were reviewed, the measurement was performed on coronal, sagittal, and axis planes after the multiplanar reformation. Nostril and mid-sagittal lines were used to locate the anterior, middle, and posterior part of ethmoidal foramina and orbital opening of OC. Further, the authors classified OC in ethmoidal sinus into 4 types by the degree of the bony defect.

Result: Distance between nostril to anterior, middle, and posterior part of ethmoidal foramina and OC are 6.23 ± 0.21, 6.62 ± 0.26, 7.29 ± 0.25, and 7.76 ± 0.41 mm, respectively. Angle between line from nostril to ethmoidal foramina and horizontal plane are 47.50° ± 1.03°, 41.67° ± 1.33°, 37.20° ± 1.34°, respectively. For the 4 types of OC, the percentage is 15.6%, 11.6%, 31.3%, and 41.5%, respectively.

Conclusions: Our findings provide anatomical information of ethmoidal foramina and OC during endoscopic transnasal approach to anterior skull base, on the basis of some fixed anatomical landmarks. So as to enhance the surgical safety of this procedure and aid in the choice of the appropriate endoscopic equipment for the procedure.
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http://dx.doi.org/10.1097/SCS.0000000000008094DOI Listing
August 2021

Debottlenecking Thermophilic Cyanobacteria Cultivation and Harvesting through the Application of Inner-Light Photobioreactor and Chitosan.

Plants (Basel) 2021 Jul 27;10(8). Epub 2021 Jul 27.

School of Environment and Energy, Peking University Shenzhen Graduate School, 2199 Lishui Rd., Shenzhen 518055, China.

Thermophilic cyanobacteria are a low-carbon environmental resource with high potential thanks to their innate temperature tolerance and thermostable pigment, phycocyanin, which enhances light utilisation efficiency and generates a high-value product. However, large-scale cultivation and harvesting have always been bottlenecks in unicellular cyanobacteria cultivation due to their micrometric size. In this study, a 40-litre inner-light photobioreactor (PBR) was designed for scaled-up cultivation of E542. By analysing light transmission and attenuation in the PBR and describing it via mathematical models, the supply of light energy to the reactor was optimised. It was found that the hyperbolic model describes the light attenuation characteristics of the cyanobacterial culture more accurately than the Lambert-Beer model. The internal illumination mode was applied for strain cultivation and showed a two-fold better growth rate and four-fold higher biomass concentration than the same strain grown in an externally illuminated photobioreactor. Finally, the downstream harvesting process was explored. A mixture of chitosan solutions was used as a flocculant to facilitate biomass collection. The effect of the following parameters on biomass harvesting was analysed: solution concentration, flocculation time and flocculant concentration. The analysis revealed that a 4 mg L chitosan solution is optimal for harvesting the strain. The proposed solutions can improve large-scale cyanobacterial biomass cultivation and processing.
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http://dx.doi.org/10.3390/plants10081540DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400073PMC
July 2021

The Fibronectin-ILT3 Interaction Functions as a Stromal Checkpoint that Suppresses Myeloid Cells.

Cancer Immunol Res 2021 Aug 23. Epub 2021 Aug 23.

NGM Biopharmaceuticals, South San Francisco, California.

Suppressive myeloid cells inhibit antitumor immunity by preventing T-cell responses. Immunoglobulin-like transcript 3 (ILT3; also known as LILRB4) is highly expressed on tumor-associated myeloid cells and promotes their suppressive phenotype. However, the ligand that engages ILT3 within the tumor microenvironment and renders tumor-associated myeloid cells suppressive is unknown. Using a screening approach, we identified fibronectin as a functional ligand for ILT3. The interaction of fibronectin with ILT3 polarized myeloid cells toward a suppressive state, and these effects were reversed with an ILT3-specific antibody that blocked the interaction of ILT3 with fibronectin. Furthermore, treatment of human tumor explants with anti-ILT3 reprogrammed tumor-associated myeloid cells toward a stimulatory phenotype. Thus, the ILT3-fibronectin interaction represents a "stromal checkpoint" through which the extracellular matrix actively suppresses myeloid cells. By blocking this interaction, tumor-associated myeloid cells may acquire a stimulatory phenotype, potentially resulting in increased antitumor T-cell responses.
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http://dx.doi.org/10.1158/2326-6066.CIR-21-0240DOI Listing
August 2021

The doping engineering and crystal structure of rod-like AuAg nanoclusters.

J Chem Phys 2021 Aug;155(7):074301

Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Alloy nanoclusters protected by ligands were widely studied due to the synergistic effect of metal atoms, and they exhibit enhanced properties in different fields, such as bio-imaging and catalysis. Herein, we obtained AuAg(PPh)Cl nanoclusters via one-step simple synthesis. The atomically precise crystal structure was determined by x-ray crystallography. It is found that the rod-like AuAg nanoclusters were composed of two AuAg icosahedrons via sharing the same Ag atom. Two Au atoms occupy the center of icosahedrons, and the other six Au atoms are all at the neck sites. Four kinds of Cl-Ag connecting modes were observed in AuAg nanoclusters. Moreover, the ultraviolet-visible absorption spectrum shows that the prominent absorption peaks of AuAg nanoclusters are at ∼395 and 483 nm. This work provides a feasible strategy to synthesize alloy nanoclusters with precise composition via doping engineering.
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http://dx.doi.org/10.1063/5.0060292DOI Listing
August 2021

Polysaccharides Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice.

Front Physiol 2021 3;12:669331. Epub 2021 Aug 3.

National-Local Joint Engineering Laboratory of Edible and Medicinal Fungi, Agricultural Resources and Environment Institute, Sichuan Academy of Agricultural Science, Chengdu, China.

This study aimed to investigate the effects of polysaccharides (MIPs) on carbon tetrachloride (CCl)-induced hepatic damage in mice. A total of 144 female mice were randomly assigned to four treatment groups, namely, control, CCl, low-dose MIP (LMIP) group, and high-dose MIP (HMIP) group. After the 10-day experiment, serum and liver were sampled for biochemical and metabolomic analyses. The HMIPs markedly decreased the liver weight under CCl intoxication. Furthermore, the significantly elevated concentrations of five serum biochemical parameters, including alanine aminotransferase, aspartate aminotransferase, triglyceride, total cholesterol, and total bile acid under CCl treatment were subverted by MIP administration in a dose-dependent manner. Moreover, MIPs relieved the increased hepatic malonaldehyde and protein carbonyl content and the decreased superoxide dismutase and catalase contents caused by CCl intoxication. There was also a dose-dependent decrease in the CCl-induced inflammatory indices, such as the levels of interleukin-1, interleukin-6, tumor necrosis factor-alpha, and myeloperoxidase, with MIP administration. Subsequent ultra-high performance liquid chromatography-tandem mass spectrometry-based serum metabolomics identified nine metabolites between the control and CCl groups and 10 metabolites between the HMIP and CCl groups, including some critical metabolites involved in flavonoid biosynthesis, amino acid metabolism, energy metabolism, and toxicant degradation. These novel findings indicate that MIPs may be of therapeutic value in alleviating the oxidative stress and inflammation caused by CCl. Liquid chromatography-mass spectrometry-based metabolomics provides a valuable opportunity for identifying potential biomarkers and elucidating the protective mechanisms of medicinal mushrooms against hepatic oxidative injury.
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http://dx.doi.org/10.3389/fphys.2021.669331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369260PMC
August 2021

Regulation of adrenergic, serotonin and dopamine receptors to inhibit diabetic retinopathy: monotherapies versus combination therapies.

Mol Pharmacol 2021 Aug 15. Epub 2021 Aug 15.

Gavin Herbert Eye Institute, Dept. of Ophthalmology, University of California, Irvine, United States.

We compared monotherapies and combinations of therapies that regulate G-protein coupled receptors (GPCR) with respect to their abilities to inhibit early stages of diabetic retinopathy (DR) in streptozotocin-diabetic mice. Metoprolol (MTP; 0.04-1.0 mg/kgbw/day), bromocriptine (BRM; 0.01-0.1 mg/kgbw/day), doxazosin (DOX; 0.01-1.0 mg/kgbw/day) or tamsulosin (TAM; 0.05-0.25 mg/kgbw/day) were injected individually daily for 2 months in dose-response studies to assess their effects on the diabetes-induced increases in retinal superoxide and leukocyte-mediated cytotoxicity against vascular endothelial cells (ECs), both of which abnormalities have been implicated in the development of DR. Each of the individual drugs inhibited the diabetes-induced increase in retinal superoxide at the higher concentrations tested, but the inhibition was lost at lower doses. To determine if combination therapies had superior effects over individual drugs, we intentionally selected for each drug a low dose that had little or no effect on the diabetes-induced retinal superoxide for use separately or in combinations in 8-month studies of retinal function, vascular permeability and capillary degeneration in diabetes. At the low doses used, combinations of the drugs generally were more effective than individual drugs, but the low dose MTP alone totally inhibited diabetes-induced reduction in a vision task, BRM or DOX alone totally inhibited the vascular permeability defect, and DOX alone totally inhibited diabetes-induced degeneration of retinal capillaries. Although low-dose MTP, BRM, DOX or TAM individually had beneficial effects on some endpoints, combination of the therapies better inhibited the spectrum of DR lesions evaluated. The pathogenesis of early stages of diabetic retinopathy remain incompletely understood, but multiple different cell-types are known to be involved in the pathogenic process. We have compared the effects of monotherapies to those of combinations of drugs that regulate G-protein coupled receptors (GPCR) signaling pathways with respect to their relative abilities to inhibit the development of early diabetic retinopathy.
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http://dx.doi.org/10.1124/molpharm.121.000278DOI Listing
August 2021

Cochlear hair cells of echolocating bats are immune to intense noise.

J Genet Genomics 2021 Jul 6. Epub 2021 Jul 6.

State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223 Yunnan, China; Center for Excellence in Animal Evolution and Genetics, Chinese Academy of Sciences, Kunming, Yunnan 650223, China. Electronic address:

Exposure to intense noise can damage cochlear hair cells, leading to hearing loss in mammals. To avoid this constraint, most mammals have evolved in relatively quiet environments. Echolocating bats, however, are naturally exposed to continuous intense sounds from their own and neighboring sonar emissions for maintaining sonar directionality and range. Here, we propose the presence of intense noise resistance in cochlear hair cells of echolocating bats against noise-induced hearing loss (NIHL). To test this hypothesis, we performed noise exposure experiments for laboratory mice, one nonecholocating bat species, and five echolocating bat species. Contrary to nonecholocating fruit bats and mice, the hearing and the cochlear hair cells of echolocating bats remained unimpaired after continuous intense noise exposure. The comparative analyses of cochleae transcriptomic data showed that several genes protecting cochlear hair cells from intense sounds were overexpressed in echolocating bats. Particularly, the experimental examinations revealed that ISL1 overexpression significantly improved the survival of cochlear hair cells. Our findings support the existence of protective effects in cochlear hair cells of echolocating bats against intense noises, which provides new insight into understanding the relationship between cochlear hair cells and intense noises, and preventing or ameliorating NIHL in mammals.
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http://dx.doi.org/10.1016/j.jgg.2021.06.007DOI Listing
July 2021

Enantioselective synthesis of chiral 3-alkyl-3-nitro-4-chromanones via chiral thiourea-catalysed intramolecular Michael-type cyclization.

Org Biomol Chem 2021 Sep 13;19(34):7403-7408. Epub 2021 Aug 13.

Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, School of Chemistry and Molecular Engineering, East China Normal University, 3663 North Zhongshan Rd., Shanghai, 200062, China.

Herein we report an enantioselective method for the rapid construction of chiral 3-nitro-4-chromanones via a chiral thiourea-catalyzed intramolecular Michael-type cyclization reaction. With this method, a series of 3,3-disubstituted-3-nitro-4-chromanones bearing contiguous C2/C3 stereocenters were obtained with high diastereoselectivities and good to excellent enantioselectivities. In vitro biological evaluations indicated that the chiral amide derivative of the product showed more potent antitumor activities than both the racemic and the corresponding enantiomers, showcasing the high influence of enantioselective methodology development toward medicinal studies.
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http://dx.doi.org/10.1039/d1ob01296gDOI Listing
September 2021

Immediate detachment of microneedles by interfacial fracture for sustained delivery of a contraceptive hormone in the skin.

J Control Release 2021 Sep 8;337:676-685. Epub 2021 Aug 8.

School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA. Electronic address:

Despite their high efficacy and safety, long-acting contraceptive methods are underutilized among women in some settings because they usually require injection or implantation by healthcare personnel. Here, we report a self-administrable microneedle (MN) patch for the rapid administration of a sustained-release contraceptive hormone delivery system into the skin that increases the simplicity and reliability of the MN delivery. We developed an immediate microneedle detachment system using a porous patch backing that has sufficient strength during MN insertion into skin under compression, but enables immediate detachment (< 1 s) of the MNs due to fracture at the MN - backing interface upon patch removal under tension from the skin surface. After patch application, the removed patch produced no biohazardous sharps waste, and was designed to achieve long-acting contraception by formulating poly(lactic-co-glycolic acid) MNs to slowly release the contraceptive hormone levonorgestrel for up to 1 month. Our combined strategy using immediate MN detachment in the skin and sustained drug delivery from the MNs could facilitate greater access to long-acting contraception by providing a simple and convenient option for self-administered, long-acting contraception.
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http://dx.doi.org/10.1016/j.jconrel.2021.08.012DOI Listing
September 2021

Preliminary investigation of microplastics in the production process of sea salt sourced from the Bohai Sea, China, using an optimised and consistent approach.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2021 Aug 9:1-14. Epub 2021 Aug 9.

School of Light Industry, Beijing Technology and Business University, Beijing, China.

In this study, we present a preliminary investigation of the characteristic changes in microplastic during the production process of sea salt in China. Five representative samples, including seawater, saturated brine, crude salt, coarse salt, and refined salt were successively collected from the production process of sea salt sourced from the Bohai Sea in China. The pretreatment method of microplastics was optimised and evaluated by the recovery rates of particle references. Microplastic characteristics in the five samples were investigated using consistent pretreatment, microscopic, and spectroscopic analyses. Results indicated that microplastics were present in every stage of sea salt production. During the "journey" of microplastics in salt production from seawater-to-seawater evaporation, to salt crystallisation, and external force processing, the abundance of microplastics with size ranging from 18 μm to 4936 μm increased substantially from 23 ± 8 to 413 ± 61 particles kg, while the proportion of larger microplastics (≥1000 μm) decreased from 33% to 9%. The results illustrate the effect of the production process on microplastic contamination of sea salt.
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http://dx.doi.org/10.1080/19440049.2021.1956691DOI Listing
August 2021

Preparation, characterization, and / evaluation of dexamethasone/poly(ε-caprolactone)-based electrode coatings for cochlear implants.

Drug Deliv 2021 Dec;28(1):1673-1684

Department of Otolaryngology Head & Neck Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

With dexamethasone as the model drug and polycaprolactone (PCL) as the carrier material, a drug delivery coating for cochlear electrodes was prepared, to control cochlear fibrosis caused by cochlear implantation. A dexamethasone/poly (ε-caprolactone)-based electrode coating was prepared using the impregnation coating method. Preparation parameters were optimized, yielding 1 impregnation instance, impregnation time of 10 s, and PCL concentration of 10%. The coating was characterized using scanning electron microscopy, a universal machine, high-performance liquid chromatography, and CCK-8. The surface was porous and uniformly thick (average thickness, 48.67 µm)-with good flexibility, long-term slow drug release, and optimal drug concentration-and was biologically safe. The experimental results show that PCL is an ideal controlled-release material for dexamethasone as a drug carrier coating for cochlear implants.
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http://dx.doi.org/10.1080/10717544.2021.1960927DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8344245PMC
December 2021

Particles and corrected particles of LDL and non-HDL are stronger predicters of coronary lesion in postmenopausal women.

BMC Cardiovasc Disord 2021 08 2;21(1):369. Epub 2021 Aug 2.

Department of Thoracic and Cardiovascular Surgery, Special Medical Center of Chinese People's Armed Police Force, 220 Chenglin Road, Tianjin, 300162, China.

Background: The optimum lipid indexes, predicting the coronary lesion in postmenopausal women are not clear.

Objective: To evaluate the optimum lipid predicter for coronary lesion in routine and advanced lipid tests.

Method: 300 postmenopausal women were enrolled and assigned into coronary heart disease (CHD) Group (242), and non-CHD Group (58). Routine and advanced lipid indexes were measured with standard laboratory test and nuclear magnetic resonance (NMR) spectroscopy. The correlation and predictivities for CHD of routine and advanced lipid indexes were performed with Logistic regression, Spearman correlation analysis and receiver operating characteristic (ROC).

Results: Age (hazard ratio (HR) 2.58, 95% confidence interval (CI) 1.08-5.86, P = 0.03), apolipoprotein B (ApoB) (HR 1.35, 95% CI 1.15-1.59, P < 0.001), corrected particles of low-density lipoprotein (LDL-p-corr) (HR 1.05, 95% CI 1.03-1.06, P < 0.001) and corrected particles of non-high-density lipoprotein (non-HDL-p-corr) (HR 1.02, 95% CI 1.01-1.03, P < 0.001) were the risk factors of CHD. LDL cholesterol (LDL-C), LDL-p, LDL-p-corr, HDL cholesterol (HDL-C), non-HDL cholesterol (non-HDL-C), non-HDL-p and non-HDL-p-corr were in linear correlation with Gensini score. Advanced lipid indexes LDL-p (area under curve (AUC) = 0.750, P = 0.02), LDL-p-corr (AUC = 0.759, P = 0.02), non-HDL-p (AUC = 0.693, P = 0.03) and non-HDL-p-corr (AUC = 0.699, P = 0.03) were more predictive for CHD than the routine ones (LDL-C and non-HDL-C).

Conclusion: In postmenopausal women, age, ApoB, LDL-p-corr and non-HDL-p-corr were risk factors of CHD. Compared with traditional lipid items, LDL-p, LDL-p-corr, non-HDL-p and non-HDL-p-corr may be better lipid indexes for CHD in postmenopausal women.
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http://dx.doi.org/10.1186/s12872-021-02189-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327454PMC
August 2021

Commensal bacteria and fungi differentially regulate tumor responses to radiation therapy.

Cancer Cell 2021 Sep 29;39(9):1202-1213.e6. Epub 2021 Jul 29.

Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; F. Widjaja Foundation Inflammatory Bowel and Immunobiology Research Institute, Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.

Studies suggest that the efficacy of cancer chemotherapy and immunotherapy is influenced by intestinal bacteria. However, the influence of the microbiome on radiation therapy is not as well understood, and the microbiome comprises more than bacteria. Here, we find that intestinal fungi regulate antitumor immune responses following radiation in mouse models of breast cancer and melanoma and that fungi and bacteria have opposite influences on these responses. Antibiotic-mediated depletion or gnotobiotic exclusion of fungi enhances responsiveness to radiation, whereas antibiotic-mediated depletion of bacteria reduces responsiveness and is associated with overgrowth of commensal fungi. Further, elevated intratumoral expression of Dectin-1, a primary innate sensor of fungi, is negatively associated with survival in patients with breast cancer and is required for the effects of commensal fungi in mouse models of radiation therapy.
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http://dx.doi.org/10.1016/j.ccell.2021.07.002DOI Listing
September 2021

The / Quorum Sensing System Effects on Pathogenicity in .

Front Microbiol 2021 12;12:679241. Epub 2021 Jul 12.

Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medicine, Jilin University, Changchun, China.

is a Gram-negative pathogen that has emerged as one of the most troublesome pathogens for healthcare institutions globally. Bacterial quorum sensing (QS) is a process of cell-to-cell communication that relies on the production, secretion, and detection of autoinducer (AI) signals to share information about cell density and regulate gene expression accordingly. The molecular and genetic bases of virulence remains poorly understood. Therefore, the contribution of the / QS system to growth characteristics, morphology, biofilm formation, resistance, motility, and virulence of was studied in detail. RNA sequencing (RNA-seq) analysis indicated that genes involved in various aspects of energy production and conversion; valine, leucine, and isoleucine degradation; and lipid transport and metabolism are associated with bacterial pathogenicity. Our work provides a new insight into the / QS system effects on pathogenicity in . We propose that targeting the acyl homoserine lactone (AHL) synthase enzyme could provide an effective strategy for attenuating virulence. On the contrary, interdicting the AI synthase receptor elicits unpredictable consequences, which may lead to enhanced bacterial virulence.
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http://dx.doi.org/10.3389/fmicb.2021.679241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312687PMC
July 2021

Extraction of crown width based on the method of optimized watershed with multi-scale markers.

Ying Yong Sheng Tai Xue Bao 2021 Jul;32(7):2449-2457

Research Center of Forestry Remote Sensing & Information Engineering, Central South University of Forestry and Technology, Changsha 410004, China.

In view of the limitation of scale on the spatial structure of ground objects and the problem that traditional watershed segmentation tends to produce crown over-segmentation, we proposed a segmentation method of crown based on the optimized watershed with multi-scale markers, with the base in Mingyue Village of Changsha County as the research object. Firstly, the high-resolution unmanned aerial vehicle (UAV) was used to collect images. The image features were analyzed to construct the classification system of , and the distribution area of was extracted. After being extracted by multi-scale region iterative growth, the crown markers were applied to the multi-threshold scale watershed transformation. Combined with Johnson index, the optimal scale of crown marker growth and watershed threshold was used to realize the accurate identification of individual trees. The results showed that the relative error between the method of optimized watershed with multi-scale markers and the visual interpretation of the reference value of tree-crown was 9.4% for the separation of individual trees. The overall identification accuracy of each tree was 89.4%, which was 34.8% higher than that of the traditional watershed segmentation method. The optimal iterative growth scale obtained by Johnson index was 20, while the thre-shold scale of watershed segmentation was 85. Compared with the results of different scale combinations, the crown extraction accuracy under the optimal scale was the highest (=0.75). The method of optimized watershed with multi-scale markers could accurately separate crown. Applying this method to UAV image crown segmentation could effectively improve the efficiency of economic forest investigation.
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http://dx.doi.org/10.13287/j.1001-9332.202107.010DOI Listing
July 2021

Natural compounds protect the skin from airborne particulate matter by attenuating oxidative stress.

Biomed Pharmacother 2021 Jun 1;138:111534. Epub 2021 Apr 1.

Department of Dermatology, West China Hospital, Sichuan University, No. 37, Guo Xue Xiang, Chengdu, Sichuan 610041, PR China; Cosmetics Safety and Efficacy Evaluation Center, West China Hospital, Sichuan University, No. 5, Gong Xing Road, Chengdu, Sichuan 610041, PR China; Sichuan Engineering Technology Research Center of Cosmetic, Chengdu, Sichuan 610041, PR China. Electronic address:

Particulate matter (PM) is a common indirect indicator of air pollution and threatens public health upon prolonged exposure, leading to oxidative stress, increasing the risk of develop respiratory and cardiovascular, as well as several autoimmune diseases and cancer. Nowadays, as a first line defense against PM, skin health attracted much attention. Our review summarized the skin damage mechanism induced by PM, including damage skin barrier directly, reactive oxygen species (ROS) accumulation, autophagy, and two canonical signaling pathways. Furthermore, ROS and oxidative stress have been considered pathogenesis centers, with essential skin damage roles. Extracts from plants and natural compounds which present high antioxidant capacity could be used to treat or protect against air pollution-related skin damage. We conclude the extracts reported in recent studies with protective effects on PM-mediated skin damage. Besides, the mechanism of extracts' positive effects has been revealed partially.
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http://dx.doi.org/10.1016/j.biopha.2021.111534DOI Listing
June 2021

CAM-DR: Mechanisms, Roles and Clinical Application in Tumors.

Front Cell Dev Biol 2021 6;9:698047. Epub 2021 Jul 6.

Medical School, Nantong University, Nantong, China.

Despite the continuous improvement of various therapeutic techniques, the overall prognosis of tumors has been significantly improved, but malignant tumors in the middle and advanced stages still cannot be completely cured. It is now evident that cell adhesion-mediated resistance (CAM-DR) limits the success of cancer therapies and is a great obstacle to overcome in the clinic. The interactions between tumor cells and extracellular matrix (ECM) molecules or adjacent cells may play a significant role in initiating the intracellular signaling pathways that are associated with cell proliferation, survival upon binding to their ligands. Recent studies illustrate that these adhesion-related factors may contribute to the survival of cancer cells after chemotherapeutic therapy, advantageous to resistant cells to proliferate and develop multiple mechanisms of drug resistance. In this review, we focus on the molecular basis of these interactions and the main signal transduction pathways that are involved in the enhancement of the cancer cells' survival. Furthermore, therapies targeting interactions between cancer cells and their environment to enhance drug response or prevent the emergence of drug resistance will also be discussed.
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http://dx.doi.org/10.3389/fcell.2021.698047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290360PMC
July 2021

Ferroptosis-Strengthened Metabolic and Inflammatory Regulation of Tumor-Associated Macrophages Provokes Potent Tumoricidal Activities.

Nano Lett 2021 08 22;21(15):6471-6479. Epub 2021 Jul 22.

School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, People's Republic of China.

Modulation of tumor-associated macrophages (TAMs) holds promise for cancer treatment, mainly relying on M1 signaling activation and pro-inflammatory promotion. Nevertheless, the antitumor activity is often limited by the anti-inflammatory factors in the tumor microenvironment. Moreover, the metabolic function of TAMs is also critical to tumor progression. However, there are a few strategies that can simultaneously regulate both inflammatory and metabolic functions to achieve safe and potent antitumor activation of TAMs. Herein, we demonstrate that an iron-based metal organic framework nanoparticle and a ferroptosis-inducing agent synergistically induce mitochondrial alternation in TAMs, resulting in a radical metabolic switch from mitochondrial oxidative phosphorylation to glycolysis, which is resistant to anti-inflammatory stimuli challenge. The ferroptosis stress strengthened by the nanoformulation also drives multiple pro-inflammatory signaling pathways, enabling macrophage activation with potent tumoricidal activities. The ferroptosis-strengthened macrophage regulation strategy present in this study paves the way for TAM-centered antitumoral treatment to overcome the limitations of conventional methods.
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http://dx.doi.org/10.1021/acs.nanolett.1c01401DOI Listing
August 2021

Ultrasound-guided implantation of radioactive I seed in radioiodine refractory differentiated thyroid carcinoma.

BMC Cancer 2021 Jul 20;21(1):834. Epub 2021 Jul 20.

Department of Ultrasound, The First Medical Center, Chinese PLA General Hospital, Beijing, China.

Background: Treatment for radioiodine refractory differentiated thyroid carcinoma (RR-DTC) is challenging. The purpose of this study was to assess the efficacy and safety of ultrasound-guided implantation of radioactive I-seed in radioiodine refractory differentiated thyroid carcinoma.

Methods: Thirty-six cervical metastatic lymph nodes (CMLNs) diagnosed with RR-DTC from 18 patients were enrolled in this retrospective study. US and contrast-enhanced ultrasound (CEUS) examinations were performed before implantation. Follow-up comprised US, CEUS, thyroglobulin (Tg) level and routine hematology at 1-3, 6, 9 and 12 months and every 6 months thereafter. The volumes of the nodules were compared before implantation and at each follow-up point. The volume reduction rate (VRR) of nodules was also recorded.

Results: The median volume of the nodules was 523 mm (148, 2010mm) initially, which decreased significantly to 53mm (0, 286mm) (P < 0.01) at the follow-up point of 24 months with a median VRR as 95% (86,100%). During the follow-up period (the range was 24-50 months), 25 (69%) nodules had VRR greater than 90%, of which 12 (33%) nodules had VVR ≈ 100% with unclear structures and only I seed images were visible in the US. At the last follow-up visit, the serum Tg level decreased from 57.0 (8.6, 114.8) ng/ml to 4.9 (0.7, 50.3) ng/ml, (P < 0.01).

Conclusion: US-guided I seed implantation is safety and efficacy in treating RR- DTC. It could be an effective supplement for the comprehensive treatment of thyroid cancer.
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http://dx.doi.org/10.1186/s12885-021-08500-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290620PMC
July 2021

Whole-exome sequencing analysis to identify novel potential pathogenetic mutations in fetuses with abnormal brain structure.

Ann Transl Med 2021 May;9(9):807

Department of Ultrasound, First Medical Center of Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.

Background: Genetic mutations in genes related to the production, migration, or differentiation of cortical neurons can result in malformations of cortical development (MCDs). However, a large number of MCD-related pathogenetic mutations remain unknown. This study aimed to investigate the genetic cause of MCDs and to identify the new MCD-associated mutations through whole-exome sequencing (WES) in fetuses with abnormal brain structure.

Methods: Cord venous blood samples were collected from 11 fetuses with MCDs. Whole-genome DNA was extracted from the blood, and WES was performed. Single nucleotide substitutions, insertions, and deletions were detected by bioinformatics analysis. Genetic mutations in genes associated with MCD were identified.

Results: A total of 1035 genes with high-impact genetic variants in at least 1 fetus were identified. The results of gene ontology enrichment analysis were consistent with those of previous studies and also indicated new potential MCD-related pathogenetic genetic mutations. Genes with high-impact mutations in multiple fetuses, such as and C-terminal binding protein 2 (), were more likely to be the genes affecting normal brain development.

Conclusions: This study has characterized variations in fetuses with MCDs and identified potential genetic mutations causing MCDs. Our findings extend the mutation spectrum of MCDs and provide a promising source for the identification of MCD-related pathogenetic mutations.
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http://dx.doi.org/10.21037/atm-21-1477DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246198PMC
May 2021

[Value of Contrast-enhanced Ultrasound Parameters in Evaluating K-W Nodule Formation in Diabetic Nephropathy].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2021 Jun;43(3):314-321

Department of Ultrasound, the First Medical Center of Chinese PLA General Hospital,Beijing 100853,China.

Objective To discuss the value of contrast-enhanced ultrasound(CEUS)parameters in evaluating the formation of Kimmelstiel-Wilson(K-W)nodules in diabetic nephropathy(DN).Methods Sixty-two patients pathologically diagnosed with DN and undergoing CEUS in the First Medical Center of Chinese PLA General Hospital from March 2017 to January 2020 were assigned into two groups according to whether K-W nodules were formed.The cortical CEUS parameters and the ratios of cortical to medullary CEUS parameters were compared between the two groups.Results The 62 patients included 19 patients without K-W nodules(group A)and 43 patients with K-W nodules(group B).The median rise time(=209,=0.013)and fall time(=197,=0.007)in group B were significantly longer than those in group A.The median wash-in rate(WiR)(=228,=0.031)and wash-out rate(WoR)(=229,=0.032)in group B were significantly lower than those in group A.The median peak enhancement(PE)1/PE2(=224,=0.026),WiR1/WiR2(=235,=0.041),and WoR1/WoR2(=230,=0.043)ratios in group B were significantly lower than those in group A.The median FT1/FT2 ratio in group B was significantly higher than that in group A(=227,=0.038).Conclusion CEUS parameters can be used to quantitatively evaluate renal cortical microperfusion in DN patients with K-W nodules.
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http://dx.doi.org/10.3881/j.issn.1000-503X.13821DOI Listing
June 2021

Synthesis and Optical Properties of Unique PtAg Nanoclusters with Mixed Exterior Motif Structures.

Inorg Chem 2021 Jul 8;60(14):10167-10172. Epub 2021 Jul 8.

Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

The atomic arrangement of metal nanoclusters plays a significant role in the structure-property correlation. Herein, we present a novel PtAg(SR)(PPh) nanocluster with a unique structure, different from two reported PtAg nanoclusters. The nanocluster was prepared via one-pot synthesis and solvent extraction. It has a centered icosahedral PtAg kernel and an open shell composed of three Ag(SR)(PPh) staple motifs and a unique trefoil-like Ag(SR) motif. The three kinds of PtAg nanoclusters have the same kernel but different shell configurations. The fine-tuning of structures is necessary and significant for the investigation of the relationship of structures and properties. The different UV-vis absorption spectra indicate that the optical properties of three PtAg nanoclusters mainly depend on the exterior shell configuration and the metal-ligand interface. This work provides insights toward growth mechanism and the structure-property correlation of metal nanoclusters.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00359DOI Listing
July 2021

Money gained through suffering is less valuable: Pain reduces the sensitivity to outcome magnitude in monetary decision making.

Soc Neurosci 2021 Jul 15:1-9. Epub 2021 Jul 15.

Magnetic Resonance Imaging Center, Center for Brain Disorders and Cognitive Neuroscience, Shenzhen University, Shenzhen, China.

Enduring pain would change individuals' behavioral preferences and neural responses in multiple decision-making tasks. Yet few studies have investigated how money's casual association with painful experience would modify people's decisions with it. It is an important and common social situation. The present study investigated how money's association with pain influences the way people make monetary decisions. Participants gambled with money that they earned in four different ways: enduring pain (Pain), randomly assigned (Random), non-painful effort task (Effort), and observing negative images (NO). Results revealed two different patterns. In the Random and Pain conditions, participants were not sensitive to the gambling risk such that they more randomly chose high- and low-risk options; the differences in FNR amplitude triggered by high- and low-risk choices were comparable on the neural level. In contrast, in the Effort and NO conditions, participants showed higher sensitivity to the magnitude and larger differences in FNR amplitudes between high- and low-risk choices. These findings suggested that pain cannot increase the subjective value of monetary gain like other non-painful efforts can do and monetary rewards may not be the optimal way to compensate for the physical suffering or loss in society.
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http://dx.doi.org/10.1080/17470919.2021.1953135DOI Listing
July 2021

FeTPPCl/FeCl Co-Catalyzed One-Pot Green Synthesis of α-Diaryl-β-alkynol Derivatives via Propargylic Carbocation Chemistry.

J Org Chem 2021 Jul 6;86(14):9306-9316. Epub 2021 Jul 6.

Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, School of Chemistry and Chemical Engineering, East China Normal University, 3663 North Zhongshan Road, Shanghai 200062, China.

A green and highly efficient one-pot method for α-diaryl-β-alkynol derivatives in water at room temperature was developed using the cocatalysis of a Lewis acid and meso-tetraphenylporphyrin iron(III) chloride (FeTPPCl). The unprecedented transformation was promoted by a modulation of the charge properties of propargylic carbocation chemistry and the use of an in situ-generated oxonium ylide as a matching nucleophile. The reaction was performed in water at room temperature with a highly step-economic manipulation in good to excellent yields and with a broad substrate scope. Water also acts as the third reactant for the one-pot transformation. Notably, the FeTPPCl catalyst can be directly reused four times with a slight discount in yields.
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http://dx.doi.org/10.1021/acs.joc.1c00474DOI Listing
July 2021
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