Publications by authors named "Jie Shen"

1,455 Publications

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Low-Dosage Bevacizumab Treatment: Effect on Radiation Necrosis After Gamma Knife Radiosurgery for Brain Metastases.

Front Surg 2021 3;8:720506. Epub 2021 Sep 3.

Department of Neurosurgery, College of Medicine, The First Affiliated Hospital, Zhejiang University, Hangzhou, China.

Cerebral radiation necrosis (RN), a complication of Gamma Knife radiosurgery, is difficult to treat, although bevacizumab seems to be effective. However, clinical data pertaining to bevacizumab treatment for RN are scarce, and its high price is problematic. This study explored the effectiveness of low-dose bevacizumab for RN caused by Gamma Knife. We retrospectively analyzed 22 patients who suffered cerebral RN post-Gamma Knife, and received bevacizumab treatment because of the poor efficacy of glucocorticoids. Low-dose bevacizumab (3 mg/kg) was administered for two cycles at 2-week intervals. T1- and T2-enhanced magnetic resonance imaging (MRI) images were examined for changes in RN status. We also monitored the dose of glucocorticoid, Karnofsky Performance Status (KPS) score, and adverse drug reactions. The mean volume of RN lesions decreased by 45% on T1-weighted images with contrast enhancement, and by 74% on T2-weighted images. All patients discontinued the use of glucocorticoids. According to the KPS scores, all patients showed an improvement in their symptoms and neurological function. No side effects were observed. Low-dosage bevacizumab at a dose of 3 mg/kg every 2 weeks is effective for treating cerebral RN after Gamma knife for brain metastases.
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http://dx.doi.org/10.3389/fsurg.2021.720506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447901PMC
September 2021

MiR-1236-3p Inhibits the Proliferation, Invasion, and Migration of Colon Cancer Cells and Hinders Epithelial-Mesenchymal Transition by Targeting DCLK3.

Front Oncol 2021 3;11:688882. Epub 2021 Sep 3.

Department of Colorectal Surgery, Ningbo Medical Center Lihuili Hospital, Ningbo City, China.

Background: Dysregulated microRNAs (miRNAs) are common in human cancer and are involved in the proliferation, promotion, and metastasis of tumor cells. Therefore, this study aimed to evaluate the expression and biological function of miR-1236-3p in colon cancer.

Methods: This study screened the miRNA in normal and colon cancer tissues through array analysis. In addition, quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) analysis was performed to validate the expression of miR-1236-3p in normal and tumor tissues from colon cancer patients and cancer cell lines. Online predicting algorithms and luciferase reporter assays were also employed to confirm Doublecortin Like Kinase 3 (DCLK3) was the target for miR-1236-3p. Moreover, the impact of miR-1236-3p on the progression of colon cancer was evaluated and . Western blotting and qRT-PCR were also performed to investigate the interactions between miR-1236-3p and DCLK3.

Results: MiR-1236-3p was significantly downregulated in colon cancer tissues and its expression was associated with the TNM stage and metastasis of colon. In addition, the and experiments showed that miR-1236-3p significantly promoted cancer cell apoptosis and inhibited the proliferation, invasion, and migration of cancer cells. The results also showed that miR-1236-3p hindered Epithelial-mesenchymal Transition (EMT) by targeting DCLK3. Moreover, the expression of DCLK3 mediated the effects of miR-1236-3p on the progression of cancer.

Conclusions: MiR-1236-3p functions as a tumor suppressor in colon cancer by targeting DCLK3 and is therefore a promising therapeutic target for colon cancer.
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http://dx.doi.org/10.3389/fonc.2021.688882DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446622PMC
September 2021

Moxibustion improves ovarian function based on the regulation of the androgen balance.

Exp Ther Med 2021 Nov 30;22(5):1230. Epub 2021 Aug 30.

Traditional Chinese Medicine Department, The Affiliated Hospital of Xuzhou Medical University, Xuzhou Medical University, Xuzhou, Jiangsu 221006, P.R. China.

The effect of androgens on follicular development and female reproduction has become an active research topic. Moxibustion is a Traditional Chinese Medicine therapy that has been reported to be able to prevent and treat numerous ovary-related problems. However, studies on the effect of moxibustion for diminished ovarian reserve (DOR) on androgen balance are still lacking. The present study aimed to assess the efficacy of moxibustion intervention prior to disease onset and at the early stage of disease in a rat model of DOR and explore the mechanisms of its effect on ovarian function. A total of 32 rats were randomly divided into four groups: Blank group, Model group (a drug-induced model of DOR), Moxibustion group 1 and Moxibustion group 2. Moxibustion was performed on the BL23 and RN4 acupoints of female rats daily for a total of 20 days (once a day, five times a week for a total of 4 weeks). The two moxibustion groups were established with different intervention times: One group was subjected to pre-disease intervention and the other group to early-disease intervention. The ovarian function was evaluated by detecting anti-Mullerian hormone (AMH), follicle-stimulating hormone (FSH), estradiol (E2), testosterone (T), dehydroepiandrosterone (DHEA), dihydrotestosterone (DHT) and androgen receptor (AR) levels in the serum or the ovary samples. To further investigate the downstream regulatory factors for AR after moxibustion treatment for pre-disease or early-disease intervention, FSH receptor (FSHR) and microRNA (miR)-125b expression in ovaries were also analyzed. The results indicated that AMH and DHT levels were reduced in the model group compared with those in the blank group, while FSH, T and DHEA levels were increased. AMH and DHT levels were increased in Moxibustion group 1 compared with those in the model group, while FSH, T and DHEA levels were reduced. There was no difference in E2 levels between Moxibustion group 1 and the model group. Compared with that in the model group, the AR content in the ovary was increased in Moxibustion group 1. There was no difference in FSHR mRNA in the ovaries between Moxibustion group 1 and the model group. miR-125b levels were significantly increased in Moxibustion group 1 as compared with those in the model group. Furthermore, AMH and DHT levels were increased in Moxibustion group 2 compared with those in the model group, while FSH, T and DHEA levels were reduced. E2 levels were significantly decreased in Moxibustion group 2 compared with those in the model group. The relative mRNA expression of AR, FSHR and miR-125b was decreased following establishment of the model. Compared with that in the model group, the AR content in the ovary was increased in Moxibustion group 2. In comparison with the blank and model groups, the FSHR content in the ovary of Moxibustion group 2 was significantly increased. miR-125b levels were not obviously altered in Moxibustion group 2 as compared with those in the model group. In addition, there was no significant difference in AMH, FSH, T and DHEA levels between the two moxibustion groups. E2 and DHT levels were higher in Moxibustion group 1 than in Moxibustion group 2. There was no difference in AR mRNA expression between the two moxibustion groups. FSHR mRNA levels were lower in Moxibustion group 1 than in Moxibustion group 2, while miR-125b mRNA levels were higher in Moxibustion group 1 than in Moxibustion group 2. In conclusion, the present study suggested that moxibustion intervention prior to disease onset and at the early disease stage was able to improve ovarian function via modulation of the AR-mediated stable equilibrium of androgens. However, the effects and mechanisms of moxibustion intervention for pre-disease and early-disease intervention of DOR appear to be different. The appropriate duration of treatment and the time-effect relationship require to be further studied.
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http://dx.doi.org/10.3892/etm.2021.10664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438671PMC
November 2021

Influence of particle-fluid density ratio on the dynamics of finite-size particles in homogeneous isotropic turbulent flows.

Phys Rev E 2021 Aug;104(2-2):025109

Key Laboratory of High Efficiency and Clean Mechanical Manufacture, Ministry of Education, School of Mechanical Engineering, Shandong University, Jinan 250061, China and National Demonstration Center for Experimental Mechanical Engineering Education, Shandong University, Jinan 250061, China.

In this paper, direct numerical simulations of particle-laden homogeneous isotropic turbulence are performed using lattice Boltzmann method incorporating interpolated bounce-back scheme. Four different particle-fluid density ratios are considered to explore how particles with different particle-fluid density ratios respond to the turbulence. Overall particle dynamics in the homogeneous isotropic turbulence such as the Lagrangian statistics of single particle and the preferential concentration of particles are investigated. Results show that particle acceleration and angular acceleration are more intermittent than velocity and angular velocity for finite-size particles with different particle-fluid density ratios. The preferential concentration of particles is investigated using radial distribution function and Voronoï tessellation, and the preferential concentration is more profound for particles with two intermediate particle-fluid density ratios. The Voronoï analysis indicates that the distribution of Voronoï cells satisfy the log-normal distribution better than the gamma distribution. The mechanism of preferential concentration is analyzed using the sweep-stick mechanism and drift mechanism. Results show that although a higher probability of having particles located near the sticky points is found, the sticky mechanism is very weak for large density ratios. The particle clustering is then found to be better qualitatively described by the drift mechanism.
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http://dx.doi.org/10.1103/PhysRevE.104.025109DOI Listing
August 2021

Paris saponin VII, a Hippo pathway activator, induces autophagy and exhibits therapeutic potential against human breast cancer cells.

Acta Pharmacol Sin 2021 Sep 14. Epub 2021 Sep 14.

Laboratory of Molecular Target Therapy of Cancer, Institute of Basic Medical Sciences, Hubei University of Medicine, Shiyan, 442000, China.

Dysregulation of the Hippo signaling pathway seen in many types of cancer is usually associated with a poor prognosis. Paris saponin VII (PSVII) is a steroid saponin isolated from traditional Chinese herbs with therapeutic action against various human cancers. In this study we investigated the effects of PSVII on human breast cancer (BC) cells and its anticancer mechanisms. We showed that PSVII concentration-dependently inhibited the proliferation of MDA-MB-231, MDA-MB-436 and MCF-7 BC cell lines with IC values of 3.16, 3.45, and 2.86 μM, respectively, and suppressed their colony formation. PSVII (1.2-1.8 μM) induced caspase-dependent apoptosis in the BC cell lines. PSVII treatment also induced autophagy and promoted autophagic flux in the BC cell lines. PSVII treatment decreased the expression and nuclear translocation of Yes-associated protein (YAP), a downstream transcriptional effector in the Hippo signaling pathway; overexpression of YAP markedly attenuated PSVII-induced autophagy. PSVII-induced, YAP-mediated autophagy was associated with increased active form of LATS1, an upstream effector of YAP. The activation of LATS1 was involved the participation of multiple proteins (including MST2, MOB1, and LATS1 itself) in an MST2-dependent sequential activation cascade. We further revealed that PSVII promoted the binding of LATS1 with MST2 and MOB1, and activated LATS1 in the BC cell lines. Molecular docking showed that PSVII directly bound to the MST2-MOB1-LATS1 ternary complex. Microscale thermophoresis analysis and drug affinity responsive targeting stability assay confirmed the high affinity between PSVII and the MST2-MOB1-LATS1 ternary complex. In mice bearing MDA-MB-231 cell xenograft, administration of PSVII (1.5 mg/kg, ip, 4 times/week, for 4 weeks) significantly suppressed the tumor growth with increased pLATS1, LC3-II and Beclin 1 levels and decreased YAP, p62 and Ki67 levels in the tumor tissue. Overall, this study demonstrates that PSVII is a novel and direct Hippo activator that has great potential in the treatment of BC.
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http://dx.doi.org/10.1038/s41401-021-00755-9DOI Listing
September 2021

Locking Compression Plate as an External Fixator for the Treatment of Tibia Infected Bone Defects.

Z Orthop Unfall 2021 Sep 8. Epub 2021 Sep 8.

Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing, China.

Purpose: This study was designed to observe the medium-term efficacy of an induced membrane technique combined with a locking compression plate as an external fixator for the treatment of tibia infected bone defects.

Methods: Patients with a tibial infection were admitted to our department between January 2013 and November 2014. All patients were treated with the induced membrane technique. In the first stage, polymethyl methacrylate (PMMA) cement was implanted in the defects after debridement and then fixed with a locking compression plate (LCP) as an external fixator. In the second stage, bone grafts were implanted to rebuild the defects. The external plates were replaced with nails in 57 patients (internal group), and the remaining 30 patients were not exchanged with fixation (external group). The infection control rate, bone union rate, and complications of the two groups were compared.

Results: Eighty-seven patients were enrolled in this study, and all patients had a minimum follow-up of 5 years (average 62.8 months) after grafting. Eighty-three patients (95.4%) achieved bone union, and the average union time was 6.77 months. Five patients (5.7%) experienced recurrence of infection. Complications included pin tract infection, fixation loosening, deformity connection, and limitation of joint range of motion (ROM). No significant differences in the infection control rate or bone defect union rate were noted between the two groups. The overall rate of complications in the external group was 50%, which was greater than that noted in the internal group (21.1%).

Conclusions: Locking compression plates are external fixators with smaller sizes that are easier to operate than conventional annular fixators or assembled external fixators. The use of locking compression plates in combination with the induced membrane technique in the treatment of tibia infected bone defects can achieve good clinical efficacy after medium-term follow-up.
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http://dx.doi.org/10.1055/a-1545-5363DOI Listing
September 2021

Application of Nanoparticle-Mediated RNAi for Efficient Gene Silencing and Pest Control on Soybean Aphids.

Authors:
Shuo Yan Jie Shen

Methods Mol Biol 2022 ;2360:307-315

Department of Plant Biosecurity and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, People's Republic of China.

The application of the RNA interference (RNAi) mechanism promotes the development of novel approaches toward sustainable crop protection. Compared with traditional double-stranded (ds)RNA delivery systems, nanoparticles offer great advantages in delivering dsRNA to improve RNAi efficiency, thus promoting the development and practice of RNAi-based pest management strategies. Here, we described a transdermal dsRNA delivery system with a nanosized star polycation, and presented a method to improve RNAi efficiency to increase the control effect against aphids. Insect gene functional analysis and pest management can be achieved by this method.
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http://dx.doi.org/10.1007/978-1-0716-1633-8_22DOI Listing
January 2022

TNF-α-Induced miR-21-3p Promotes Intestinal Barrier Dysfunction by Inhibiting MTDH Expression.

Front Pharmacol 2021 12;12:722283. Epub 2021 Aug 12.

Department of Emergency and Critical Care Medicine, Jinshan Hospital, Fudan University, Shanghai, China.

Intestinal barrier dysfunction is characterized by increased intestinal permeability to lumen endotoxin, showing remarkable predisposition to immune enteropathy, and colorectal cancer tumor necrosis factor (TNF)-α is associated with this pathological process, while the mechanism remains unknown. In this study, different doses of TNF-α were used for Caco-2 cell treatment. We discovered that miR-21-3p expression was obviously increased by TNF-α in a dose-dependent manner. Further study demonstrated that TNF-α could upregulate miR-21-3p expression through the NF-κB signaling pathway. Then, TargetScan and miRWalk miRNA-mRNA interaction prediction online tools were introduced, and metadherin (MTDH) was screened out as a potential target of miR-21-3p. We subsequently found that miR-21-3p could directly target the 3'-untranslated region (UTR) of MTDH mRNA and inhibit its expression. Furthermore, it was demonstrated that miR-21-3p could regulate the Wnt signaling pathway by targeting MTDH mRNA, suggesting the effect of miR-21-3p/MTDH/Wnt axis on intestinal barrier dysfunction. Our findings provide a novel potential biomarker and therapeutic target for intestinal barrier dysfunction and related diseases.
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http://dx.doi.org/10.3389/fphar.2021.722283DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415152PMC
August 2021

Human gut microbiome impacts skeletal muscle mass via gut microbial synthesis of the short-chain fatty acid butyrate among healthy menopausal women.

J Cachexia Sarcopenia Muscle 2021 Sep 1. Epub 2021 Sep 1.

Center for System Biology, Data Sciences, and Reproductive Health, School of Basic Medical Science, Central South University, Changsha, Hunan, China.

Background: Increasing evidence suggests that human gut microbiome plays an important role in variation of skeletal muscle mass (SMM). However, specific causal mechanistic relationship of human gut microbiome with SMM remains largely unresolved. Understanding the causal mechanistic relationship may provide a basis for novel interventions for loss of SMM. This study investigated whether human gut microbiome has a causal effect on SMM among Chinese community-dwelling healthy menopausal women.

Methods: Estimated SMM was derived from whole-body dual-energy X-ray absorptiometry. We performed integrated analyses on whole-genome sequencing, shotgun metagenomic sequencing, and serum short-chain fatty acids (SCFAs), as well as available host SMM measurements among community-dwelling healthy menopausal women (N = 482). We combined the results with summary statistics from genome-wide association analyses for human gut microbiome (N = 952) and SMM traits (N = 28 330). As a prerequisite for causality, we used a computational protocol that was proposed to measure correlations among gut metagenome, metabolome, and the host trait to investigate the relationship between human gut microbiome and SMM. Causal inference methods were applied to assess the potential causal effects of gut microbial features on SMM, through one-sample and two-sample Mendelian randomization (MR) analyses, respectively.

Results: In metagenomic association analyses, the increased capacity for gut microbial synthesis of the SCFA butyrate was significantly associated with serum butyrate levels [Spearman correlation coefficient (SCC) = 0.13, P = 0.02] and skeletal muscle index (SCC = 0.084, P = 0.002). Of interest was the finding that two main butyrate-producing bacterial species were both positively associated with the increased capacity for gut microbial synthesis of butyrate [Faecalibacterium prausnitzii (SCC = 0.25, P = 6.6 × 10 ) and Butyricimonas virosa (SCC = 0.15, P = 0.001)] and for skeletal muscle index [F. prausnitzii (SCC = 0.16, P = 6.2 × 10 ) and B. virosa (SCC = 0.17, P = 2.4 × 10 )]. One-sample MR results showed a causal effect between gut microbial synthesis of the SCFA butyrate and appendicular lean mass (β = 0.04, 95% confidence interval 0.029 to 0.051, P = 0.003). Two-sample MR results further confirmed the causal effect between gut microbial synthesis of the SCFA butyrate and appendicular lean mass (β = 0.06, 95% confidence interval 0 to 0.13, P = 0.06).

Conclusions: Our results may help the future development of novel intervention approaches for preventing or alleviating loss of SMM.
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http://dx.doi.org/10.1002/jcsm.12788DOI Listing
September 2021

Single-cell transcriptome analysis of diffuse large B cells in cerebrospinal fluid of central nervous system lymphoma.

iScience 2021 Sep 11;24(9):102972. Epub 2021 Aug 11.

Department of Clinical Laboratory, Huashan Hospital, Fudan University, Shanghai, China.

Diffuse large B cells in the cerebrospinal fluid (CSF-DLBCs) have offered great promise for the diagnostics and therapeutics of central nervous system lymphoma (CNSL) leptomeningeal involvement. To explore the phenotypic states of CSF-DLBCs, we analyzed the transcriptomes of more than one thousand CSF-DLBCs from six patients with CNSL diffuse large B-cell lymphoma (DLBCL) using Smart-seq2 single-cell RNA sequencing. CSF-DLBCs were defined based on abundant expression of B-cell markers, the active cell proliferation and energy metabolism properties, and immunoglobulin light chain restriction. We identified inherent heterogeneity of CSF-DLBCs, which were mainly manifested in cell cycle state, cancer-testis antigen expression, and classification based on single-cell germinal center B-cell signature. In addition, the 16 upregulated genes in CSF-DLBCs compared to various normal B cells showed great possibility in the homing effect of the CNS-DLBCL for the leptomeninges. Our results will provide insight into the mechanism research and diagnostic direction of CNSL-DLBCL leptomeningeal involvement.
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http://dx.doi.org/10.1016/j.isci.2021.102972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387906PMC
September 2021

An Avascular Niche Created by Axitinib-Loaded PCL/Collagen Nanofibrous Membrane Stabilized Subcutaneous Chondrogenesis of Mesenchymal Stromal Cells.

Adv Sci (Weinh) 2021 Aug 28:e2100351. Epub 2021 Aug 28.

Department of Pediatric Cardiology, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, No.1678 Dongfang Road, Shanghai, 200127, P. R. China.

Engineered cartilage derived from mesenchymal stromal cells (MSCs) always fails to maintain the cartilaginous phenotype in the subcutaneous environment due to the ossification tendency. Vascular invasion is a prerequisite for endochondral ossification during the development of long bone. As an oral antitumor medicine, Inlyta (axitinib) possesses pronounced antiangiogenic activity, owing to the inactivation of the vascular endothelial growth factor (VEGF) signaling pathway. In this study, axitinib-loaded poly(ε-caprolactone) (PCL)/collagen nanofibrous membranes are fabricated by electrospinning for the first time. Rabbit-derived MSCs-engineered cartilage is encapsulated in the axitinib-loaded nanofibrous membrane and subcutaneously implanted into nude mice. The sustained and localized release of axitinib successfully inhibits vascular invasion, stabilizes cartilaginous phenotype, and helps cartilage maturation. RNA sequence further reveals that axitinib creates an avascular, hypoxic, and low immune response niche. Timp1 is remarkably upregulated in this niche, which probably plays a functional role in inhibiting the activity of matrix metalloproteinases and stabilizing the engineered cartilage. This study provides a novel strategy for stable subcutaneous chondrogenesis of mesenchymal stromal cells, which is also suitable for other medical applications, such as arthritis treatment, local treatment of tumors, and regeneration of other avascular tissues (cornea and tendon).
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http://dx.doi.org/10.1002/advs.202100351DOI Listing
August 2021

Sustained oxygenation accelerates diabetic wound healing by promoting epithelialization and angiogenesis and decreasing inflammation.

Sci Adv 2021 Aug 27;7(35). Epub 2021 Aug 27.

Department of Mechanical Engineering and Materials Science, Washington University in St. Louis, St. Louis, MO 63130, USA.

Nonhealing diabetic wounds are common complications for diabetic patients. Because chronic hypoxia prominently delays wound healing, sustained oxygenation to alleviate hypoxia is hypothesized to promote diabetic wound healing. However, sustained oxygenation cannot be achieved by current clinical approaches, including hyperbaric oxygen therapy. Here, we present a sustained oxygenation system consisting of oxygen-release microspheres and a reactive oxygen species (ROS)-scavenging hydrogel. The hydrogel captures the naturally elevated ROS in diabetic wounds, which may be further elevated by the oxygen released from the administered microspheres. The sustained release of oxygen augmented the survival and migration of keratinocytes and dermal fibroblasts, promoted angiogenic growth factor expression and angiogenesis in diabetic wounds, and decreased the proinflammatory cytokine expression. These effects significantly increased the wound closure rate. Our findings demonstrate that sustained oxygenation alone, without using drugs, can heal diabetic wounds.
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http://dx.doi.org/10.1126/sciadv.abj0153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397271PMC
August 2021

Unilateral Subfrontal Approach for Giant Tuberculum Sellae Meningioma: Single Center Experience and Review of the Literature.

Front Oncol 2021 9;11:708235. Epub 2021 Aug 9.

Department of Neurosurgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Microsurgical Transcranial approach (mTCA) is the primary choice for the resection of giant Tuberculum Sellae Meningiomas (TSM). The objective of this study is to explore surgical details of unilateral subfrontal approach.

Methods: Ten patients with giant TSM treated by unilateral subfrontal approach were included from January 2018 to June 2021. Demographic characteristics, surgical data, post-procedure complications and outcomes of patients have been descriptive analyzed, combined with systematic literature review to explore the surgical details and the prognosis of unilateral subfrontal approach.

Results: Ten patients include six male and four females, age range from 35 to 77 years, duration of visual impairment from 1 to 12 months, were all performed unilateral subfrontal approach. Nine patients achieved radical resection (Simpson grades I-II) through post-operative imaging confirmation, and Simpson IV resection was performed in the remaining one due to cavernous sinus invasion. The postoperative visual acuity was improved or maintained in 8 patients. Visual acuity decreased in 2 cases, including 1 case of optic nerve atrophy and the other case of optic canal not opening. Five cases with frontal sinus opened were repaired during the operation and there was no postoperative cerebrospinal fluid leakage or intracranial infection. One patient suffered from postoperative anosmia, one patient developed left limb weakness, but their symptoms have improved in the follow-up.

Conclusion: Summarize the experience of our center and previous literature, unilateral forehead bottom craniotomy is a feasible surgical approach for giant tuberculum sellae meningioma. Intraoperative application of EC glue and pedicled fascia flap to repair the frontal sinus can prevent complications associated with frontal sinus opening. Optic canal unroofing has huge advantage in visual improvement.
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http://dx.doi.org/10.3389/fonc.2021.708235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381050PMC
August 2021

Ionic Functionalization of Multivariate Covalent Organic Frameworks to Achieve an Exceptionally High Iodine-Capture Capacity.

Angew Chem Int Ed Engl 2021 Aug 24. Epub 2021 Aug 24.

Advanced Membranes and Porous Materials (AMPM) Center, Physical Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.

Adsorption-based iodine (I ) capture has great potential for the treatment of radioactive nuclear waste. In this study, we apply a "multivariate" synthetic strategy to construct ionic covalent organic frameworks (iCOFs) with a large surface area, high pore volume, and abundant binding sites for I capture. The optimized material iCOF-AB-50 exhibits a static I uptake capacity of 10.21 g g at 75 °C and a dynamic uptake capacity of 2.79 g g at ≈400 ppm I and 25 °C, far exceeding the performances of previously reported adsorbents under similar conditions. iCOF-AB-50 also exhibits fast adsorption kinetics, good moisture tolerance, and full reusability. The promoting effect of ionic groups on I adsorption has been elucidated by experimentally identifying the iodine species adsorbed at different sites and calculating their binding energies. This work demonstrates the essential role of balancing the textural properties and binding sites of the adsorbent in achieving a high I capture performance.
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http://dx.doi.org/10.1002/anie.202108522DOI Listing
August 2021

Deletion of Glut1 in early postnatal cartilage reprograms chondrocytes toward enhanced glutamine oxidation.

Bone Res 2021 Aug 23;9(1):38. Epub 2021 Aug 23.

Department of Orthopaedic Surgery, School of Medicine, Washington University, St. Louis, MO, USA.

Glucose metabolism is fundamental for the functions of all tissues, including cartilage. Despite the emerging evidence related to glucose metabolism in the regulation of prenatal cartilage development, little is known about the role of glucose metabolism and its biochemical basis in postnatal cartilage growth and homeostasis. We show here that genetic deletion of the glucose transporter Glut1 in postnatal cartilage impairs cell proliferation and matrix production in growth plate (GPs) but paradoxically increases cartilage remnants in the metaphysis, resulting in shortening of long bones. On the other hand, articular cartilage (AC) with Glut1 deficiency presents diminished cellularity and loss of proteoglycans, which ultimately progress to cartilage fibrosis. Moreover, predisposition to Glut1 deficiency severely exacerbates injury-induced osteoarthritis. Regardless of the disparities in glucose metabolism between GP and AC chondrocytes under normal conditions, both types of chondrocytes demonstrate metabolic plasticity to enhance glutamine utilization and oxidation in the absence of glucose availability. However, uncontrolled glutamine flux causes collagen overmodification, thus affecting extracellular matrix remodeling in both cartilage compartments. These results uncover the pivotal and distinct roles of Glut1-mediated glucose metabolism in two of the postnatal cartilage compartments and link some cartilage abnormalities to altered glucose/glutamine metabolism.
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http://dx.doi.org/10.1038/s41413-021-00153-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382841PMC
August 2021

Fine Structure and Olfactory Reception of the Labial Palps of .

Front Physiol 2021 3;12:680697. Epub 2021 Aug 3.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

The olfactory system of insects is essential in many crucial behaviors, such as host seeking, mate recognition, and locating oviposition sites. Lepidopteran moths possess two main olfactory organs, including antennae and labial palps. Compared to antennae, the labial palps are relatively specific and worthy of further investigation due to the labial-palp pit organ (LPO), which contains a large number of sensilla located on the tip segment. The fall armyworm, , is a worldwide lepidopteran pest, which can damage more than 350 plants and cause significant economic losses. In this study, we surveyed the structure of the labial palps and LPO of using a super-high magnification lens zoom 3D microscope. Then, the distribution and fine structure of sensilla located in the LPO of were investigated using scanning electron microscopy. Subsequently, the electrophysiological responses of labial palps to CO and 29 plant volatiles were recorded by using electrolabialpalpography. Our results showed the fine structure of labial palps, the LPO, and the sensilla located in the LPO of . Moreover, we demonstrated that the labial palps are olfactory organs that respond to both CO and other volatile compounds. Our work established a foundation for further study of the roles of labial palps in insect olfactory related behaviors. Further investigations on the function of labial palps and their biological roles together with CO and volatile compound responses in are necessary, as they may provide better insect behavioral regulators for controlling this pest.
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http://dx.doi.org/10.3389/fphys.2021.680697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369160PMC
August 2021

Antibiotic cement plate composite structure internal fixation after debridement of bone infection.

Sci Rep 2021 Aug 19;11(1):16921. Epub 2021 Aug 19.

Department of Orthopaedics, First Affiliated Hospital, Third Military Medical University (Army Medical University), Gaotanyan No. 30, Chongqing, 400038, The People's Republic of China.

An internal fixation composite structure of antibiotic cement plates was created. The aim of this study was to analyse the infection control effect of this structure when applied to treat a bone infection. We retrospectively analysed patients with bone infection admitted to our hospital between January 2013 and June 2019. After debridement, an antibiotic cement plate composite structure was used to fill and stabilize the defects. The treatment effect was evaluated at six months after surgery, and the infection control rate, factors associated with the recurrence of infection, and complications were analysed. If the patients had bone defects, the defect was repaired after infection control, and the infection control rate of all of the patients was re-evaluated at 12 months after surgery. A total of 548 patients were treated with this technique, including 418 men and 130 women. The infection sites included 309 tibias, 207 femurs, 16 radii and ulnae, 13 humeri, and 3 clavicles. After at least 6 months of follow-up, 92 patients (16.79%) had an infection recurrence and needed further treatment. The recurrence rate of the tibia was higher than that of the femur (P = 0.025). Eighty-nine out of 92 patients who relapsed underwent a second debridement with the same method, and the infection control rate after the second debridement was 94.71%. Complications included 8 cases of epidermal necrosis around the incision, 6 cases of internal fixation failure, and 30 cases of lower limb swelling. By the follow-up time of 12 months, another 6 patients had experienced recurrence of infection, and 4 cases were controlled after debridement. Finally, among all 548 cases, 7 patients remained persistently infected, and 6 underwent amputation. The infection control rate was 97.6% at the 1-year follow-up. The clinical efficacy of this new antibiotic cement plate composite structure for internal fixation after debridement of bone infection is stable and reliable.
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http://dx.doi.org/10.1038/s41598-021-96522-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377006PMC
August 2021

[Establishment of a field visual detection method for genetically modified maize 'Shuangkang'12-5 by fluorescence RPA].

Yi Chuan 2021 Aug;43(8):802-812

tate Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

The genetically modified (GM) maize 'Shuangkang'12-5 has good insect resistance and herbicide tolerance, which is one of the first series of GM maizes obtained a safety certificate in China, and it has broad application prospect in the future. This study established an on-site rapid detection method for GM 'Shuangkang'12-5 based on recombinase polymerase amplification (RPA) technology, which primes and probe were designed according to the specific flank sequence. Then the best combination of primers and probe was obtained through a screeing process. The amplification results of fluorescence RPA can be directly visualized under blue light. The results showed that the visual detection system of GM 'Shuangkang'12-5 with high specificity, and the detection sensitivity of the method could reached 10 copies. Further research found that the RPA amplification system had a wide range of temperature (34℃-46℃). According to this property, the common self-heating warm pastes on the market were used replace the traditional heating instruments to stimulate the RPA.The results showed that the self-heating warm paste meets the temperature requirement of the RPA system. Finally, we combined the self-heating warm pastes with the RPA visual detection system to conduct on-site detection of GM 'Shuangkang'12-5, and compared the results with the detection results of qPCR. The detection showed that the results of on-site visual detection method established in this study were consistent with the detection results of the qPCR. Moreover, the visual detection method was more shorter in time and the final detection result was clear and easy to distinguish. The rapid on-site visual detection method for GM 'Shuangkang' 12-5 established in this study has high specificity, high sensitivity and convenience. It not only meets the needs of on-site rapid detection of GM 'Shuangkang'12-5, but also provides highlight for the development of other on-site rapid detection methods.
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http://dx.doi.org/10.16288/j.yczz.21-110DOI Listing
August 2021

Characteristics, treatment patterns, and glycemic control of older type 2 diabetes mellitus patients in China.

Chin Med J (Engl) 2021 Aug 17. Epub 2021 Aug 17.

Department of Endocrinology and Metabolism, Peking University People's Hospital, Beijing 100044, China Department of Medical Affairs, Boehringer Ingelheim (China) Investment Co., Ltd., Shanghai 200040, China Department of Biostatistics & Data Sciences Asia, Boehringer Ingelheim (China) Investment Co., Ltd., Shanghai 200040, China Department of Global Epidemiology, Boehringer Ingelheim (China) Investment Co., Ltd., Beijing 100027, China Boehringer Ingelheim International GmbH, Binger Strasse 173, 55216 Ingelheim am Rhein, Germany.

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http://dx.doi.org/10.1097/CM9.0000000000001674DOI Listing
August 2021

Using a stable isotope-labeled internal standard for liquid chromatography-tandem mass spectrometry quantitation of meloxicam in human plasma.

Biomed Chromatogr 2021 Jul 19:e5217. Epub 2021 Jul 19.

Anhui Provincial Center for Drug Clinical Evaluation, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui, People's Republic of China.

A sensitive and highly efficient LC-ESI-MS/MS method using a stable isotope-labeled internal standard (SIL IS) to detect meloxicam in human plasma was developed and validated. Sample preparation used only 50 μL human plasma with one-step methanol protein precipitation. A gradient mobile phase system was adopted for chromatographic separation on a Poroshell 120 SB-C column (2.1 × 50 mm, 2.7 μm). Positive ion pattern was chosen for quantification under multiple reaction monitoring. Ion pairs were [M + H] m/z 352.1 → 115.1 for meloxicam and [M + H] m/z 355.1 → 187.1 for meloxicam-d3 (SIL IS). Total run time was 4.0 min. Standard curve was linear over a concentration range from 8.00 to 1600 ng mL . This method was fully validated to evaluate its performance, including specificity, carryover, sensitivity, linearity, accuracy, precision, recovery, matrix effects, stability, dilution reliability and incurred sample reanalysis, which provided a reliable basis for pharmacokinetic studies of meloxicam in 28 healthy Chinese volunteers. After a single-dose oral administration of 7.5 mg meloxicam, the main pharmacokinetic parameters were as follows: C , 814.79 ± 201.37 ng mL ; T , 4.54 ± 1.42 h; AUC , 24,572.04 ± 5766.93 ng·h mL ; AUC , 25,810.89 ± 6796.60 ng·h mL and t , 21.11 ± 5.35 h.
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http://dx.doi.org/10.1002/bmc.5217DOI Listing
July 2021

Effects of Chinese herbal medicines on lifespan in Drosophila.

Exp Gerontol 2021 Aug 13;154:111514. Epub 2021 Aug 13.

College of Life Information Science & Instrument Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.

Food ingredients have shown beneficial effect in delaying aging and extend lifespan. There are Chinese herbal medicines in the category of "homology of medicine and food". In order to find out whether these herbal medicines can act as food component to slow aging, this study selected 12 Chinese herbal medicines containing strong antioxidant components, Canarium album, Amomum villosum, Elsholtzia splendens, Foeniculum vulgare, Fructus hordei germinatus, stir-baked Fructus hordei germinatus, Lilium brownie, Citrus medica, Sophora japonica, Myristica fragrans, Herba houttuyniae, Carthamus tinctoriu, and examined the effects on lifespan using Drosophila melanogaster as the model organism. Our results show that the extracts of the 12 Chinese herbal medicines have various effects on longevity. Some reduced the lifespan in both sexes. Some only shortened the lifespan in one sex. Some have no significant effect in both sexes. There are two herbal medicine extended lifespan, but only in females. The present results suggest that herbal medicines may provide potential candidates for anti-aging ingredients.
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http://dx.doi.org/10.1016/j.exger.2021.111514DOI Listing
August 2021

Group membership moderates the process of making trust judgments based on facial cues.

J Soc Psychol 2021 Aug 17:1-12. Epub 2021 Aug 17.

Department of Psychology, Ningbo University, Ningbo, Zhejiang Province, China.

Trust is a foundation of interpersonal communication. Faces have a significant impact on trust judgments, and separate research demonstrates that group membership also influences trust judgments. However, it remains unclear whether and how group membership moderates the effect of face trustworthiness on trust judgments and investment decisions. In the present research, two experiments were conducted to explore the moderating effect of group membership (i.e., in-group vs. out-group) on perceptions of facial trustworthiness and trust judgments. Results showed that participants invested significantly more money on trials with trustworthy faces than trials with untrustworthy faces. Additionally, there was a significant interaction between group membership and facial trustworthiness; the investment difference between trustworthy faces and untrustworthy faces was greater for trials with in-group member faces than out-group member faces. These findings indicate that top-down and bottom-up cues jointly influence behavioral decisions.
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http://dx.doi.org/10.1080/00224545.2021.1939249DOI Listing
August 2021

Challenges for graduate students' mentors during COVID-19 pandemic.

Biochem Mol Biol Educ 2021 Aug 14. Epub 2021 Aug 14.

College of Life Information Science and Instrument Engineering, Hangzhou Dianzi University, Hangzhou, China.

The outbreak of COVID-19 pandemic results in the suspension of universities' academic laboratory activities. This brought a difficult time for graduate students majored in molecular biology and their mentors, since the student's thesis progress relies on the data from wet experiments in the lab. Even facing the challenges during the pandemic, mentors could still play an active role. This COVID-19 crisis requires mentors to have more thinking, innovation, and sharing in guiding graduate students.
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http://dx.doi.org/10.1002/bmb.21568DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426749PMC
August 2021

Shadow-wall lithography of ballistic superconductor-semiconductor quantum devices.

Nat Commun 2021 Aug 13;12(1):4914. Epub 2021 Aug 13.

QuTech and Kavli Institute of Nanoscience, Delft University of Technology, Delft, The Netherlands.

The realization of hybrid superconductor-semiconductor quantum devices, in particular a topological qubit, calls for advanced techniques to readily and reproducibly engineer induced superconductivity in semiconductor nanowires. Here, we introduce an on-chip fabrication paradigm based on shadow walls that offers substantial advances in device quality and reproducibility. It allows for the implementation of hybrid quantum devices and ultimately topological qubits while eliminating fabrication steps such as lithography and etching. This is critical to preserve the integrity and homogeneity of the fragile hybrid interfaces. The approach simplifies the reproducible fabrication of devices with a hard induced superconducting gap and ballistic normal-/superconductor junctions. Large gate-tunable supercurrents and high-order multiple Andreev reflections manifest the exceptional coherence of the resulting nanowire Josephson junctions. Our approach enables the realization of 3-terminal devices, where zero-bias conductance peaks emerge in a magnetic field concurrently at both boundaries of the one-dimensional hybrids.
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http://dx.doi.org/10.1038/s41467-021-25100-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363628PMC
August 2021

Synergy of surface sodium and hydroxyl on NaTiHO nanotubes accelerating the Pt-dominated ambient HCHO oxidation.

J Hazard Mater 2021 Jul 31;421:126769. Epub 2021 Jul 31.

Department of Materials Engineering, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada.

Surface hydroxyl is widely perceived as conducive to HCHO degradation. Here, a kind of sodium titanate with interlayered hydroxyls (NaTiHO) was prepared to study the action conditions of surface hydroxyls in HCHO oxidation. The nanotubes mainly exposing (001) and nanobelts mainly exposing (100) are synthesized as the two morphologies of NaTiHO. We found the (001) facet is much more favored to HCHO adsorption via HRTEM and XPS analysis. The DFT calculations prove that the synergy of surface hydroxyl and Na atom is perfect for HCHO chemisorption. By this means NaTiHO nanotubes can partially oxidize HCHO into formate and release very few CO, measured by in situ DRIFTS. Dominated by Pt nanoparticles, the complete oxidation of HCHO can be performed on NaTiHO nanotubes with enhanced early reaction speed. Rather than simple surface hydroxyl, the effective synergy of hydroxyl and positive ion is proposed as an advantage for HCHO oxidation.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126769DOI Listing
July 2021

High and Economical Nattokinase Production with Acetoin as a Useful Byproduct from Soybean Milk and Glucose.

Probiotics Antimicrob Proteins 2021 Aug 13. Epub 2021 Aug 13.

Center for Bioengineering and Biotechnology, College of Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, China.

Nattokinase (NK) is a potent fibrinolytic enzyme with wide pharmaceutical and nutraceutical applications. Safe and high NK-yielding strains are urgently needed. In this study, the best strain NDF was isolated from one of the 11 natto samples and then identified as Bacillus subtilis. The effects of carbon and nitrogen sources on NK production were investigated, and glucose and soybean milk were finally selected as the optimal carbon and nitrogen sources, respectively. Acetoin, a valuable compound with versatile usages, was detected as the main byproduct of carbon overflow. In a 6-L fermenter, NK and acetoin reached their peak concentrations simultaneously (10,220 IU/mL and 25.9 g/L, respectively) at 25 h in a culture medium containing 180 g/L of soybean milk and 105 g/L of glucose. The NK product was verified by sequencing of the aprN gene and SDS-PAGE analysis. Only very limited kinds of proteins were found in the supernatant of the fermentation broth, and NK was one of the main bands. This study has developed an economical and high NK production method with acetoin as a useful byproduct.
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http://dx.doi.org/10.1007/s12602-021-09831-2DOI Listing
August 2021

A Study on Seizure Detection of EEG Signals Represented in 2D.

Sensors (Basel) 2021 Jul 29;21(15). Epub 2021 Jul 29.

School of Computer Science and Technology, Hangzhou Dianzi University, Hangzhou 310018, China.

A seizure is a neurological disorder caused by abnormal neuronal discharges in the brain, which severely reduces the quality of life of patients and often endangers their lives. Automatic seizure detection is an important research area in the treatment of seizure and is a prerequisite for seizure intervention. Deep learning has been widely used for automatic detection of seizures, and many related research works decomposed the electroencephalogram (EEG) raw signal with a time window to obtain EEG signal slices, then performed feature extraction on the slices, and represented the obtained features as input data for neural networks. There are various methods for EEG signal decomposition, feature extraction, and representation, and most of the studies have been based on fixed hardware resources for the design of the scheme, which reduces the adaptability of the scheme in different application scenarios and makes it difficult to optimize the algorithms in the scheme. To address the above issues, this paper proposes a deep learning-based model for seizure detection, mainly characterized by the two-dimensional representation of EEG features and the scalability of neural networks. The model modularizes the main steps of seizure detection and improves the adaptability of the model to different hardware resource constraints, in order to increase the convenience of the algorithm optimization or the replacement of each module. The proposed model consists of five parts, and the model was tested using two epilepsy datasets separately. The experimental results showed that the proposed model has strong generality and good classification accuracy for seizure detection.
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http://dx.doi.org/10.3390/s21155145DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348755PMC
July 2021

Association between body mass index, its change and cognitive impairment among Chinese older adults: a community-based, 9-year prospective cohort study.

Eur J Epidemiol 2021 Aug 9. Epub 2021 Aug 9.

School of Public Health, Zhejiang University School of Medicine, Hangzhou, 310058, Zhejiang, China.

To examine the association of baseline body mass index (BMI) and BMI change with cognitive impairment among older adults in China. The study included data from the Chinese Longitudinal Healthy Longevity Study, a national community-based prospective cohort study from 2002 to 2018. Baseline BMI and BMI change were available for 12,027 adults aged older than 65 years. Cognitive impairment was defined as Chinese version of the Mini Mental State Examination score lower than 18. Multivariable Cox proportional hazard model was used. Among 12,027 participants (mean age was 81.23 years old and 47.48% were male), the proportion of underweight, normal, overweight and obese at baseline was 33.87%, 51.39%, 11.39% and 3.34%, respectively. During an average of 5.9 years' follow-up, 3086 participants (4.35 per 100 person-years) with incident cognitive impairment were identified. Compared with normal weight group, adjusted hazard ratio (AHR) for cognitive impairment was 0.86 (95% CI 0.75-0.99) among overweight group, whereas corresponding AHR was 1.02 (95% CI 0.94-1.10) in underweight and 1.01 (95% CI 0.80-1.28) in obese participants. Large weight loss (< -10%) was significantly associated with an increased risk of cognitive impairment (AHR, 1.42, 95% CI 1.29-1.56), compared to stable weight status group (-5% ~ 5%). In the restricted cubic spline models, BMI change showed a reverse J-shaped association with cognitive impairment. BMI-defined overweight, but not obesity, was associated with a lower risk of cognitive impairment among elderly Chinese adults, while large weight loss was associated with an increased risk. These findings are consistent with weight loss in the prodromal phase of dementia.
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http://dx.doi.org/10.1007/s10654-021-00792-yDOI Listing
August 2021

Metabolite Profiling Based on UPLC-Q-TOF-MS/MS and the Biological Evaluation of Medicinal Plants of Chinese Dichocarpum (Ranunculaceae).

Chem Biodivers 2021 Aug 5:e2100432. Epub 2021 Aug 5.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100193, P. R. China.

The genus Dichocarpum is endemic to East Asia, and many species have been used to treat various diseases. However, phytochemical researches of this genus have been limited to date. In the present study, a metabolomic approach based on UPLC-Q-TOF-MS/MS was used to explore the phytochemical profiles of 10 Chinese Dichocarpum species, and cannabinoid receptor (CB1/CB2) agonistic activities evaluation of these plants was performed. A total of 128 features were putatively annotated, belonging to alkaloids, flavonoids, triterpenes saponins, phenolic acids, and others. Semi-quantitative statistics demonstrated that alkaloids and flavonoids were widely distributed, with the former the most abundant, whereas triterpenes saponins were mainly distributed in D. fargesii and D. wuchuanense. The phylogenetic results obtained from DNA sequencing assigned the 10 species to three groups. Further results of in silico annotation revealed three chemical families and helped determine the characteristic features of the three groups. In addition, the plant extracts of nine species from this genus showed agonistic activity on CB2 receptors. This comprehensive analysis revealed the chemotype distribution and pharmacophylogenetic relationship, to provide clues for the prospective resource utilization of the medicinal plants from the genus Dichocarpum.
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http://dx.doi.org/10.1002/cbdv.202100432DOI Listing
August 2021
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