Publications by authors named "Jie Qiu"

256 Publications

Oxidative stress in the skin: impact and related protection.

Int J Cosmet Sci 2021 Jul 27. Epub 2021 Jul 27.

L'Oreal Research and Innovation, 550 Jin Yu Road, Pudong Shanghai, China, 201206.

Skin, our first interface to the external environment, is subjected to oxidative stress caused by a variety of factors such as solar ultraviolet, infrared, and visible light, environmental pollution, including ozone and particulate matters, and psychological stress. Excessive reactive species, including reactive oxygen species and reactive nitrogen species, exacerbate skin pigmentation and aging, which further lead to skin tone unevenness, pigmentary disorder, skin roughness, and wrinkles. Besides these, skin microbiota is also a very important factor ensuring the proper functions of skin. While environmental factors such as UV and pollutants impact skin microbiota compositions, skin dysbiosis results in various skin conditions. In this review, we summarize the generation of oxidative stress from exogenous and endogenous sources. We further introduce current knowledge on the possible roles of oxidative stress in skin pigmentation and aging, specifically with emphasis on oxidative stress and skin pigmentation. Meanwhile, we summarize the science and rationale of using three well-known antioxidants, namely vitamin C, resveratrol, and ferulic acid, in the treatment of hyperpigmentation. Finally, we discuss the strategy for preventing oxidative stress-induced skin pigmentation and aging.
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http://dx.doi.org/10.1111/ics.12728DOI Listing
July 2021

Four Biomarkers-Based Artificial Neural Network Model for Accurate Early Prediction of Bacteremia with Low-level Procalcitonin.

Ann Clin Lab Sci 2021 May;51(3):408-414

Department of Laboratory Medicine, Mindong Hospital of Ningde City, Fuan City, China.

Objective: Procalcitonin levels above 2.0 ng/mL are associated with a higher risk of severe sepsis. Bacteremia with procalcitonin levels lower than 2.0 ng/mL has not received much attention, and relevant prediction models are lacking. Herein, a panel of biomarkers was used to establish an early predictive model for bacteremia using machine learning approaches.

Methods: Retrospective evaluation of 487 non-bacteremia controls and 444 bacteremia patients with low-level procalcitonin was performed. Clinical data, including procalcitonin, interleukin-6, C-reactive protein, D-dimer, and lactic acid levels, as well as leukocyte, neutrophil, and platelet counts, were used to identify a panel of relevant variables to build a machine learning model.

Results: By comparing six prediction models, the performance of an artificial neural network (ANN) was found to be superior to that of other designed models, with a sensitivity of 0.82, a specificity of 0.85, and an accuracy rate of 83.5%. Furthermore, interleukin-6, procalcitonin, D-dimer, and lactic acid were found to be the most influential biomarkers with the potential to predict bacteremia.

Conclusion: The ANN model described herein holds outstanding predictive performance, with the potential to provide real-time, data-driven predictions of bacteremia.
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May 2021

Investigation of absolute dose calibration accuracy for TomoTherapy using real water.

J Appl Clin Med Phys 2021 Jun 31;22(6):139-145. Epub 2021 May 31.

Department of Radiation Medicine, University of Kentucky, Lexington, Kentucky, USA.

A systematic bias in TomoTherapy output calibration was reported by the Imaging and Radiation Oncology Core Houston (IROC-H) after analyzing intensity-modulated radiation therapy (IMRT) credentialing results from hundreds of TomoTherapy units. Multiple theories were developed to explain this observation. One theory was that the use of a solid water "cheese" phantom instead of real water in the calibration measurement was the culprit. A phantom filled with distilled water was built to investigate whether our TomoTherapy was miscalibrated due to the use of a solid water phantom. A miscalibration of -1.47% was detected on our TomoTherapy unit. It is found that despite following the vendor's updated recommendation on computed tomography (CT) number to density calibration, the cheese phantom was still mapped to a density of 1.028 g/cm , rather than the 1.01 g/cm value reported in literature. When the density of the cheese phantom was modified to 1.01 g/cm in the treatment planning system, the measurement also indicated that our TomoTherapy machine was miscalibrated by -1.52%, agreeing with the real water phantom findings. Our single-institution finding showed that the cheese phantom density assignment can introduce greater than 1% errors in the TomoTherapy absolute dose calibration. It is recommended that the absolute dose calibration for TomoTherapy be performed either in real water or in the cheese phantom with the density in TPS overridden as 1.01 g/cm .
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http://dx.doi.org/10.1002/acm2.13311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200510PMC
June 2021

Integrated Analysis of ceRNA Network Reveals Prognostic and Metastasis Associated Biomarkers in Breast Cancer.

Front Oncol 2021 13;11:670138. Epub 2021 May 13.

Department of Breast Surgery, Zhejiang Provincial People's Hospital, Hangzhou, China.

Background: Breast cancer is a malignancy and lethal tumor in women. Metastasis of breast cancer is one of the causes of poor prognosis. Increasing evidences have suggested that the competing endogenous RNAs (ceRNAs) were associated with the metastasis of breast cancer. Nonetheless, potential roles of ceRNAs in regulating the metastasis of breast cancer remain unclear.

Methods: The RNA expression (3 levels) and follow-up data of breast cancer and noncancerous tissue samples were downloaded from the Cancer Genome Atlas (TCGA). Differentially expressed and metastasis associated RNAs were identified for functional analysis and constructing the metastasis associated ceRNA network by comprehensively bioinformatic analysis. The Kaplan-Meier (K-M) survival curve was utilized to screen the prognostic RNAs in metastasis associated ceRNA network. Moreover, we further identified the metastasis associated biomarkers with operating characteristic (ROC) curve. Ultimately, the data of Cancer Cell Line Encyclopedia (CCLE, https://portals.broadinstitute.org/ccle) website were selected to obtained the reliable metastasis associated biomarkers.

Results: 1005 mRNAs, 22 miRNAs and 164 lncRNAs were screened as differentially expressed and metastasis associated RNAs. The results of GO function and KEGG pathway enrichment analysis showed that these RNAs are mainly associated with the metabolic processes and stress responses. Next, a metastasis associated ceRNA (including 104 mRNAs, 19 miRNAs, and 16 lncRNAs) network was established, and 12 RNAs were found to be related to the overall survival (OS) of patients. In addition, 3 RNAs (hsa-miR-105-5p, BCAR1, and PANX2) were identified to serve as reliable metastasis associated biomarkers. Eventually, the results of mechanism analysis suggested that BCAR1 might promote the metastasis of breast cancer by facilitating Rap 1 signaling pathway.

Conclusion: In the present research, we identified 3 RNAs (hsa-miR-105-5p, BCAR1 and PANX2) might associated with prognosis and metastasis of breast cancer, which might be provide a new perspective for metastasis of breast cancer and contributed to the treatment of breast cancer.
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http://dx.doi.org/10.3389/fonc.2021.670138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158160PMC
May 2021

Determining optimal gestational weight gain (GWG) in a northwest Chinese population: A CONSORT.

Medicine (Baltimore) 2021 May;100(21):e26080

Gansu Provincial Maternity and Child Care Hospital, Qilihe District, Lanzhou, Gansu, China.

Abstract: To determine optimal gestational weight gain (GWG) for the Chinese population.Live singleton deliveries at the largest maternal & childcare hospital in northwest China from 2010 to 2012 were analyzed retrospectively. Multivariable logistic regression analysis was conducted to determine the lowest aggregated risk of interested perinatal outcomes based on Chinese adult body mass index (BMI) categories.Eight thousand eight hundred seventy enrolled parturients were divided into 4 groups according to their prepregnancy BMI: underweight (21.31%, BMI < 18.5 kg/m2), normal weight (67.81%, 18.5 kg/m2 ≤ BMI < 24 kg/m2), overweight (8.99%, 24 kg/m2 ≤ BMI < 28 kg/m2 and obese (1.89%, BMI ≥ 28 kg/m2). The optimal GWG values for the above 4 groups were 16.7 kg (GWG range, 12.0-21.5), 14.5 kg (9.5-19.5), 11.5 kg (7.0-16.5), and 8.0 kg (5.0-13.0). The rates of inadequate, optimal and excessive GWG in present study were 6.14% (545), 62.34% (5529), and 31.52% (2796) respectively, which were significantly different from those of the 2009 Institute of Medicine recommendation (χ2 = 1416.05, Pinteraction < 0.0001).Wider optimal GWG ranges than those recommended by Institute of Medicine were found in our study, and our proposed criteria seems to be practical to the Chinese population.
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http://dx.doi.org/10.1097/MD.0000000000026080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154430PMC
May 2021

Magnolol attenuates inflammatory pain by inhibiting sodium currents in mouse dorsal root ganglion neurons.

Inflammopharmacology 2021 Jun 22;29(3):869-877. Epub 2021 May 22.

Key Laboratory of Medical Electrophysiology, Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research of Southwest, Medical University, Luzhou, 646000, China.

Voltage-gated sodium channels are currently recognized as one of the targets of analgesics. Magnolol (Mag), an active component isolated from Magnolia officinalis, has been reported to exhibit analgesic effects. The objective of this study was to investigate whether the analgesic effect of Mag was associated with blocking Na channels. Inflammatory pain was induced by the injection of carrageenan into the hind paw of mice. Mag was administered orally. Mechanical hyperanalgesia was evaluated by using von Frey filaments. Na currents and neuronal excitability in acutely isolated mouse dorsal root ganglion (DRG) neurons were recorded with the whole-cell patch clamp technique. Results showed that Mag (10 ~ 40 mg/kg) dose-dependently inhibited the paw edema and reduced mechanical pain in the inflammatory animal model. Injection of carrageenan significantly increased the amplitudes of TTX-sensitive and TTX-resistant Na currents. Compared with the carrageenan group, Mag inhibited the upregulation of two types of Na currents induced by carrageenan in a dose-dependent manner. Mag 40 mg/kg shifted the inactivation curves of two types of Na currents to hyperpolarization and returned to normal animal level without changing their activation curves. Mag 40 mg/kg significantly reduced the percentage of cells firing multiple spikes and inhibited the neuronal hyperexcitability induced by carrageenan. Our data suggest that the analgesic effect of Mag may be associated with a decreased neuronal excitability by blocking Na current.
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http://dx.doi.org/10.1007/s10787-021-00809-8DOI Listing
June 2021

miRNA-10a-5p inhibits cell metastasis in hepatocellular carcinoma via targeting SKA1.

Kaohsiung J Med Sci 2021 May 17. Epub 2021 May 17.

Medical School, Southeast University, Nanjing, China.

A variety of microRNAs (miRNAs) are involved in the occurrence and development of hepatocellular carcinoma (HCC). However, the role of miR-10a-5p in the progression of HCC remains unclear. Therefore, the purpose of this study was to determine the role of miR-10a-5p in the development of HCC and the possible molecular mechanism. miR-10a-5p expression in HCC tissues and plasma from patients was detected by quantitative real-time polymerase chain reaction. Migratory changes in HCC cells were detected after the overexpression of miR-10a-5p. Epithelial-mesenchymal transition (EMT)-related proteins were detected by Western blot. Finally, through luciferase assay and rescue experiments, the mechanism by which miR-10a-5p regulates its downstream gene, human spindle and kinetochore-associated complex subunit 1, SKA1 and the interaction between these molecules in the development of HCC were determined. The expression of miR-10a-5p was markedly downregulated in HCC tissues, cell lines, and plasma. The overexpression of miR-10a-5p significantly inhibited the migration, invasion, and EMT of HCC cells. Furthermore, SKA1 was shown to be a downstream gene of miR-10a-5p. SKA1 silencing had the same effect as miR-10a-5p overexpression in HCC. In particular, the overexpression of SKA1 reversed the inhibitory effects of miR-10a-5p in HCC. Taken together, low miR-10a-5p expression is associated with HCC progression. miR-10a-5p inhibits the malignant development of HCC by negatively regulating SKA1.
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http://dx.doi.org/10.1002/kjm2.12392DOI Listing
May 2021

Efficacy and safety of phytoestrogens in the treatment of perimenopausal and postmenopausal depressive disorders: A systematic review and meta-analysis.

Int J Clin Pract 2021 May 13:e14360. Epub 2021 May 13.

Key Laboratory of Evidence Based Medicine and Knowledge Translation of Gansu Province, Lanzhou, PR China.

Background: Depression is one of the most common and specific symptoms among menopausal women, leading to significant personal, family, and economic burdens. Some studies have shown that phytoestrogens can help relieve symptoms of depression.

Objectives: This systematic review and meta-analysis aim to assess the efficacy and safety of phytoestrogens in treating depression among menopausal women.

Methods: A comprehensive search for relevant studies published until November 25, 2020, was conducted in PubMed, the Cochrane Library, Chinese Biomedical Literature Database, Web of Science, and EMBASE. Statistical analyses were performed with R 4.0.3.and Review Manager 5.4.

Results: 2183 studies were identified and 10 studies with 15 independent reports were included, involving 1248 participants. The quality of the four studies was assessed as high risk, six studies were assessed as unclear. The analyses conducted according random effects model indicated the significant positive effect on depressive symptoms for postmenopausal women compared with the placebo (SMD = -0.62; 95% CI = -1.13 to -0.12; Q = 45.62, df = 14, P < .01; I  = 79%). The low dose phytoestrogens (25 mg/d ≤ dose ≤ 100 mg/d) have better effectiveness (SMD = -0.52; 95% CI = -0.85 to -0.20; I  = 79%, P< .01) than high dose (dose > 100 mg/d) and ultralow dose (0 < dose < 25 mg/d), but showed no statistical significance (Q = 0.81 df = 2, P = .67). Isoflavones had better effectiveness (SMD = -0.48; 95% CI = -0.75 to 0.21; I  = 75%, P < .01) than lignans of phytoestrogens (SMD = -0.22; 95% CI = -0.37 to 0.08; I  = 0%, P = .96). The duration of intervention affects the efficacy of phytoestrogens (β = -0.03; 95% CI: [-0.05, 0.00]; P = .045). The effectiveness varies in regions. The adverse reactions frequently reported were gastrointestinal symptoms and cold or upper respiratory tract infection.

Conclusions: Phytoestrogen can relieve depression symptoms among menopausal, especially for postmenopausal women who take low doses(25 mg/d ≤ dose ≤ 100 mg/d) of phytoestrogens for a long-term duration. Although mild adverse reactions have been reported, phytoestrogen could be considered as a complementary treatment for postmenopausal depression.
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http://dx.doi.org/10.1111/ijcp.14360DOI Listing
May 2021

Hydrogen sulfide improves ox‑LDL‑induced expression levels of Lp‑PLA in THP‑1 monocytes via the p38MAPK pathway.

Mol Med Rep 2021 05 24;23(5). Epub 2021 Mar 24.

Department of Cardiology Laboratory, First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.

Hydrogen sulfide (HS) exerts an anti‑atherosclerotic effect and decreases foam cell formation. Lipoprotein‑associated phospholipase A2 (Lp‑PLA) is a key factor involved in foam cell formation. However, the association between HS and Lp‑PLA expression levels with respect to foam cell formation has not yet been elucidated. The present study investigated whether HS can affect foam cell formation and potential signalling pathways via regulation of the expression and activity of Lp‑PLA. Using human monocytic THP‑1 cells as a model system, it was observed that oxidized low‑density lipoprotein (ox‑LDL) not only upregulates the expression level and activity of Lp‑PLA, it also downregulates the expression level and activity of Cystathionine γ lyase. Exogenous supplementation of HS decreased the expression and activity of Lp‑PLA induced by ox‑LDL. Moreover, ox‑LDL induced the expression level and activity of Lp‑PLA via activation of the p38MAPK signalling pathway. HS blocked the expression levels and activity of Lp‑PLA induced by ox‑LDL via inhibition of the p38MAPK signalling pathway. Furthermore, HS inhibited Lp‑PLA activity by blocking the p38MAPK signaling pathway and significantly decreased lipid accumulation in ox‑LDL‑induced macrophages, as detected by Oil Red O staining. The results of the present study indicated that HS inhibited ox‑LDL‑induced Lp‑PLA expression levels and activity by blocking the p38MAPK signalling pathway, thereby improving foam cell formation. These findings may provide novel insights into the role of HS intervention in the progression of atherosclerosis.
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http://dx.doi.org/10.3892/mmr.2021.11997DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974332PMC
May 2021

Effective optical smoothing scheme to suppress laser plasma instabilities by time-dependent polarization rotation via pulse chirping.

Opt Express 2021 Jan;29(2):1304-1319

In this paper, we propose a novel effective optical smoothing scheme to suppress laser plasma instabilities (LPIs) by time-dependent polarization rotation (TPR) on a picosecond timescale. The polarization rotation with time-dependent frequency is generated by the superposition of chirped light pulses with dynamic frequency shift and counter-rotating circular polarization. Compared to light without polarization rotation or pulse chirping, such superposed light with TPR has a broader spectrum and lower temporal coherence. Using the one-dimensional fluid laser-plasma-instability code (FLAME) and PIC simulation, TPR is demonstrated working well in suppressing parametric backscattering, which provides an effective approach to suppress LPIs. In the meantime, a significant improvement of irradiation uniformity of the chirped pulses is achieved by the introduction of proper spatial phase modulation and grating dispersion.
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http://dx.doi.org/10.1364/OE.405319DOI Listing
January 2021

Hyperglycemia and Chemoresistance in Breast Cancer: From Cellular Mechanisms to Treatment Response.

Front Oncol 2021 25;11:628359. Epub 2021 Feb 25.

Department of Breast Surgery, Zhejiang Provincial People's Hospital, Hangzhou, China.

Female breast cancer is a complex, multifactorial disease. Studies have shown that hyperglycemia is one of the most important contributing factors to increasing the risk of breast cancer that also has a major impact on the efficacy of chemotherapy. At the cellular level, hyperglycemia can promote the proliferation, invasion, and migration of breast cancer cells and can also induce anti-apoptotic responses to enhance the chemoresistance of tumors abnormal glucose metabolism. In this article, we focus on the latest progress in defining the mechanisms of chemotherapy resistance in hyperglycemic patients including the abnormal behaviors of cancer cells in the hyperglycemic microenvironment and the impact of abnormal glucose metabolism on key signaling pathways. To better understand the advantages and challenges of breast cancer treatments, we explore the causes of drug resistance in hyperglycemic patients that may help to better inform the development of effective treatments.
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http://dx.doi.org/10.3389/fonc.2021.628359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947364PMC
February 2021

Improvement on storage stability of iron-fortified soybean powder by microencapsulation.

J Food Sci 2021 Mar 10;86(3):996-1003. Epub 2021 Feb 10.

Ferguson (Wuhan) Biotechnology Co., Ltd., Wuhan, 430056, China.

Full-fat soybean powder was a more difficult-to-fortify food vehicle than cereal flour and powdered milk products because of a large quantity of unsaturated fatty acids, particularly when iron was necessary to be fortified. Minimizing oxidation of lipids was extremely valuable in the fortified-food industry. However, very limited data were available on the effect of microencapsulation of iron compounds on lipid oxidation in full-fat soybean powder. In our study, ferric pyrophosphate (FP) was microencapsulated by the emulsifying-gelation technique and its effect on the storage stability of Yingyangbao (YYB) was evaluated. The results showed that microencapsulated FP (MFP) was regularly spherical and uniformly distributed. MFP could significantly (P < 0.05) decrease the sensory score of rancid odor for YYB. The formation of lipid oxidation products such as carbonyl compounds, malondialdehyde, pentanal, and hexanal in YYB during the accelerated test was significantly retarded, improving oxidative stability and delaying the sensory deterioration. The E-nose analysis showed that YYB with MFP had significantly (P < 0.05) lower levels of response values on the specific sensors than YYB containing FP with or without ascorbyl palmitate. MFP could significantly (P < 0.05) improve the sensory and oxidative stability of iron-fortified full-fat soybean powder such as YYB.
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http://dx.doi.org/10.1111/1750-3841.15621DOI Listing
March 2021

A quantitative genomics map of rice provides genetic insights and guides breeding.

Nat Genet 2021 02 1;53(2):243-253. Epub 2021 Feb 1.

Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, China.

Extensive allelic variation in agronomically important genes serves as the basis of rice breeding. Here, we present a comprehensive map of rice quantitative trait nucleotides (QTNs) and inferred QTN effects based on eight genome-wide association study cohorts. Population genetic analyses revealed that domestication, local adaptation and heterosis are all associated with QTN allele frequency changes. A genome navigation system, RiceNavi, was developed for QTN pyramiding and breeding route optimization, and implemented in the improvement of a widely cultivated indica variety. This work presents an efficient platform that bridges ever-increasing genomic knowledge and diverse improvement needs in rice.
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http://dx.doi.org/10.1038/s41588-020-00769-9DOI Listing
February 2021

Modulations of Na1.8 and Na1.9 Channels in Monosodium Urate-Induced Gouty Arthritis in Mice.

Inflammation 2021 Aug 29;44(4):1405-1415. Epub 2021 Jan 29.

Department of Clinical Pharmacy, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing City, 210009, Jiangsu Province, China.

The aim of the present study was to observe the changes of TTX-R, Na1.8, and Na1.9 Na currents in MSU-induced gouty arthritis mice, and to explore the possibility of Na1.8 and Na1.9 channels as potential targets for gout pain treatment. Acute gouty arthritis was induced by monosodium urate (MSU) in mice. Swelling degree was evaluated by measuring the circumference of the ankle joint. Mechanical allodynia was assessed by applying the electronic von Frey. Na currents were recorded by patch-clamp techniques in acute isolated dorsal root ganglion (DRG) neurons. MSU treatment significantly increased the swelling degree of ankle joint and decreased the mechanical pain threshold. The amplitude of TTX-R Na current was significantly increased and reached its peak on the 4th day after injection of MSU. For TTX-R Na channel subunits, Na1.8 current density was significantly increased, but Na1.9 current density was markedly decreased after MSU treatment. MSU treatment shifted the steady-state activation curves of TTX-R Na channel, Na1.8 and Na1.9 channels, and the inactivation curves of TTX-R Na channel and Na1.8 channels to the depolarizing direction, but did not affect the inactivation curve of Na1.9 channel. Compared with the normal group, the recovery of Na1.8 channel was faster, while that of Na1.9 channel was slower. The recovery of TTX-R Na channel remained unchanged after MSU treatment. Additionally, MSU treatment increased DRG neurons excitability by reducing action potential threshold. Na1.8 channel, not Na1.9 channel, may be involved in MSU-induced gout pain by increasing nerve excitability.
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http://dx.doi.org/10.1007/s10753-021-01425-yDOI Listing
August 2021

Exosomal miR-365a-5p derived from HUC-MSCs regulates osteogenesis in GIONFH through the Hippo signaling pathway.

Mol Ther Nucleic Acids 2021 Mar 10;23:565-576. Epub 2020 Dec 10.

Department of Orthopedics, The Provincial Hospital Affiliated to Shandong University, Shandong 250014, China.

The pathogenesis of glucocorticoid (GC)-induced osteonecrosis of the femoral head (GIONFH) is still disputed, and abnormal bone metabolism caused by GCs may be an important factor. , Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) staining were used to evaluate cellular proliferation, and western blotting was used to investigate osteogenesis. , we used micro-computed tomography (micro-CT), H&E staining, Masson staining, and immunohistochemistry (IHC) analysis to evaluate the impact of exosomes. In addition, the mechanism by which exosomes regulate osteogenesis through the miR-365a-5p/Hippo signaling pathway was investigated using RNA sequencing (RNA-seq), luciferase reporter assays, fluorescence hybridization (FISH), and western blotting. The results of western blotting verified that the relevant genes in osteogenesis, including BMP2, Sp7, and Runx2, were upregulated. RNA-seq and qPCR of the exosome and Dex-treated exosome groups showed that miR-365a-5p was upregulated in the exosome group. Furthermore, we verified that miR-365a-5p promoted osteogenesis by targeting SAV1. Additional experiments revealed that exosomes prevented GIONFH in a rat model, as shown by micro-CT scanning and histological and IHC analysis. We concluded that exosomal miR-365a-5p was effective in promoting osteogenesis and preventing the development of GIONFH via activation of the Hippo signaling pathway in rats.
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http://dx.doi.org/10.1016/j.omtn.2020.12.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810916PMC
March 2021

External beam radiotherapy for prostate cancer: What are the current research trends and hotspots?

Cancer Med 2021 01 21;10(2):772-782. Epub 2021 Jan 21.

Department of Radiation Oncology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Background: The external beam radiotherapy (EBRT) applied for prostate cancer (PCa) has been one of the most important and hottest research fields over recent decades. This study aimed to explore the research hotspots of EBRT in PCa and help the researchers have a clear and intuitive reference basis for later researches.

Methods: The literature scientometric analysis related to "EBRT applied for PCa" was conducted via the Web of Science Core Collection from 2010 to 2019. The Microsoft Office Excel 2019 and CiteSpace V. 5.7.R1 software were introduced for visualizing and analyzing the data.

Results: A total of 7860 relevant papers were extracted and downloaded. A total of 7828 papers were extracted and analyzed after data cleansing by CiteSpace. The tendency of published papers was comprehensively increasing from 2010 to 2019. Among all 73 countries/regions, USA published the most papers, accounting for 39%, which was the most active contributor with most publications. Australia (Centrality: 0.18), England (Centrality: 0.12) were cooperating most cohesively with other countries. Univ Toronto was the most productive institute (229), while Harvard Univ (Centrality: 0.67) had extensive collaborations with other institutes. The International journal of Radiation Oncology Biology Physics had the largest number of publications and the highest number of co-citations. Briganti A had the largest volume of publications. D'Amico AV had the highest number of co-citations. Four latest and largest clusters were identified as oligometastases, salvage therapy (SRT), prostate-specific membrane antigen (PSMA), and hypofractionation. Thirteen references became strongest burst citations lasting until 2019. The studies of "oligometastases," "SRT," "PSMA," "hypofractionation," "postoperative radiotherapy," and "dose and fraction regimen changes" were prevailing in the recent years.

Conclusion: The "oligometastases," "SRT," "PSMA," "hypofractionation," "postoperative radiotherapy," and "dose and fraction regimen changes" may be the state-of-art research frontiers, and related studies will advance in this field over time.
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http://dx.doi.org/10.1002/cam4.3700DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877352PMC
January 2021

Cooking stoves and risk of birth defects in urban China.

Environ Res 2021 03 13;194:110731. Epub 2021 Jan 13.

National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address:

Introduction: Birth defects are a leading cause of infant death. Pregnant women spend a large amount of time indoors, and little research from population-based studies has investigated the association between indoor air pollution and birth defects. We aimed to examine whether using coal, biomass, or electromagnetic stoves for cooking is associated with risk of birth defects compared to using gas stoves.

Methods: A birth cohort study was conducted from 2010 to 2012 in Lanzhou, China. Cases (n = 264) were singleton births with birth defects, which were defined as abnormalities of structure or function, including metabolism, presented at birth based on the International Classification of Diseases (ICD)-10 codes. Controls (n = 9926) were defined as singleton live births without birth defects. Unconditional logistic regression models were employed to estimate the association adjusting for confounding variables.

Results: Compared to gas stoves for cooking, biomass (OR = 2.66, 95%CI: 1.38-5.13), and electromagnetic stove (OR = 1.90, 95%CI: 1.26-2.88) for cooking were associated with an increased risk of birth defects. The significant associations remained among non-congenital heart disease (CHD) defects but not CHDs.

Conclusions: Using biomass or electromagnetic stoves for cooking during pregnancy was associated with an increased risk of birth defects. Additional studies are warranted to confirm these novel findings. Studies with larger sample size or greater statistical power are also warranted to better estimate the associations for individual birth defects.
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http://dx.doi.org/10.1016/j.envres.2021.110731DOI Listing
March 2021

Genome-wide identification of agronomically important genes in outcrossing crops using OutcrossSeq.

Mol Plant 2021 04 8;14(4):556-570. Epub 2021 Jan 8.

Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China. Electronic address:

Many important crops (e.g., tuber, root, and tree crops) are cross-pollinating. For these crops, no inbred lines are available for genetic study and breeding because they are self-incompatible, clonally propagated, or have a long generation time, making the identification of agronomically important genes difficult, particularly in crops with a complex autopolyploid genome. In this study, we developed a method, OutcrossSeq, for mapping agronomically important loci in outcrossing crops based on whole-genome low-coverage resequencing of a large genetic population, and designed three computation algorithms in OutcrossSeq for different types of outcrossing populations. We applied OutcrossSeq to a tuberous root crop (sweet potato, autopolyploid), a tree crop (walnut tree, highly heterozygous diploid), and hybrid crops (double-cross populations) to generate high-density genotype maps for the outcrossing populations, which enable precise identification of genomic loci underlying important agronomic traits. Candidate causative genes at these loci were detected based on functional clues. Taken together, our results indicate that OutcrossSeq is a robust and powerful method for identifying agronomically important genes in heterozygous species, including polyploids, in a cost-efficient way. The OutcrossSeq software and its instruction manual are available for downloading at www.xhhuanglab.cn/tool/OutcrossSeq.html.
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http://dx.doi.org/10.1016/j.molp.2021.01.003DOI Listing
April 2021

Effectiveness of Gentle Human Touch for Pain Control During Examination for Retinopathy of Pre-maturity: A Randomized Controlled Trial.

Front Pediatr 2020 17;8:608378. Epub 2020 Dec 17.

Department of Neonates, Children's Hospital of Nanjing Medical University, Nanjing, China.

Retinopathy of pre-maturity (ROP) is a disorder of the retinal blood vessels in pre-term infants with low birth weight. It is a leading cause of blindness in children. During ROP screening, the use of mydriatic drops and eyelid openers causes pain and discomfort. Pain management strategies include medications and behavioral interventions. The objectives of this study was to investigate the effects of Gentle Human Touch on pain in pre-term infants undergoing screening for ROP. In this randomized controlled trial, 82 infants in the neonatal intensive care unit at Children's Hospital of Nanjing Medical University who met the ROP screening criteria were randomly assigned to experimental and control groups using the random number table. The infants in the experimental group continuously received Gentle Human Touch during screening, while those in the control group were screened according to the routine procedure. All neonates were administered local eye anesthesia before the screening. The degree of pain was assessed using the Pre-mature Infant Pain Profile score. A double-channel near-infrared spectroscopy device was used to monitor regional cerebral oxygen saturation (rScO), while oxygen saturation (SaO) and heart rate were measured using pulse oximetry. The Pre-mature Infant Pain Profile score was the primary outcome, while heart rate, SaO, and rScO were the secondary outcomes. The gestational age, corrected gestational age, birth weight, and Apgar score at examination and the basal heart rate, SaO, and rScO showed no significant intergroup differences ( > 0.05 for all). Both groups demonstrated significant decreases in SaO and rScO in response to the examination ( < 0.05 for all). During the examination, the Pre-mature Infant Pain Profile score (14.82 ± 3.22 vs. 9.29 ± 2.89, respectively; < 0.05) was significantly higher in the control group than in the experimental group, while rScO (57.61 ± 3.51 vs. 54.76 ± 4.54%, respectively; < 0.05) and SaO (91.89 ± 6.43 vs. 85.68 ± 8.31%; < 0.05) were significantly higher in the experimental group than in the control group. There was no significant difference in heart rate changes between the two groups before and after the examination (182.60 ± 3.50 vs. 170.80 ± 3.50 time/min; > 0.05). The findings of this study suggest that Gentle Human Touch can effectively alleviate pain during ROP screening in pre-mature infants. ISRCTN10976481, Registered 06 March 2020, Retrospectively registered.
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http://dx.doi.org/10.3389/fped.2020.608378DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793661PMC
December 2020

Paternal factors and adverse birth outcomes in Lanzhou, China.

BMC Pregnancy Childbirth 2021 Jan 6;21(1):19. Epub 2021 Jan 6.

Gynaecology department, Gansu Provincial Maternity and Child Care Hospital, Lanzhou, Gansu, China.

Background: Many maternal factors are known to be associated with adverse birth outcomes, but studies about paternal factors yielded inconsistent conclusions. The study was to assess whether paternal factors are associated with low birth weight (LBW), preterm birth (PTB), and small for gestational age (SGA).

Methods: A birth cohort study was conducted in 2010-2012 at the Gansu Provincial Maternity and Child Care Hospital, the largest maternity and childcare hospital in Lanzhou, China. Paternal age, ethnicity, educational level, height, weight, smoking, and drinking were collected. Birth outcomes and pregnancy complications were extracted from the medical records.

Results: During the study period, 10,121 participants were included; the overall prevalence of LBW, PTB, and SGA was 7.2, 9.9, and 7.8%, respectively. Paternal higher height (OR = 0.64 95%CI: 0.49, 0.83), higher weight (P for trend < 0.001), and higher BMI (P for trend < 0.001) could decrease the rate of LBW. Paternal higher education (OR = 0.55, 95%CI: 0.43, 0.71) and higher weight (P for trend < 0.001,) were associated with lower rate of PTB. Fathers who smoked more than 6 pack-years were associated with PTB (OR = 1.31, 95%CI: 1.07, 1.61). Paternal BMI > 23.9 kg/m (P for trend < 0.001,) and paternal education which above college (OR = 0.61, 95%CI: 0.50, 0.82) were associated with a lower rate of SGA.

Conclusion: Paternal low education is independently associated with PTB and SGA. Paternal heavy smoking is associated with PTB. Low paternal weight/BMI is independently associated with LBW, PTB, and SGA.
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http://dx.doi.org/10.1186/s12884-020-03492-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789361PMC
January 2021

The role of diagnostic magnetic resonance hysterosalpingography in the evaluation of fallopian tubal occlusion of female infertility: A meta-analysis.

Clin Imaging 2021 Apr 6;72:11-18. Epub 2020 Nov 6.

Evidence-based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu, China. Electronic address:

Objective: To evaluate the diagnostic performance of magnetic resonance hysterosalpingography for fallopian tubal occlusion in the context of female infertility when compared to the diagnostic performance of conditional X-ray hysterosalpingography.

Methods: PubMed, EMBASE, Web of Science, EBSCO, Cochrane Library database, Scopus were searched for studies in which magnetic resonance hysterosalpingography and X-ray hysterosalpingography were used as diagnostic tools for tubal occlusion assessment; databases were searched through April 2020. Two researchers conducted study inclusion assessment, data extraction, a systematic review, and pooled meta-analysis independently. Stata 15.1 software was used to analyze the pooled sensitivity, specificity, diagnostic odds ratio, positive and negative likelihood ratios, and the area under the summary receiver-operating characteristic curve of magnetic resonance hysterosalpingography.

Results: A total of five studies involving 101 patients and 198 fallopian tubes were finally included. Compared with the conditional X-ray hysterosalpingography (the imaging gold standard), the pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio and the area under the curve of magnetic resonance hysterosalpingography for tubal occlusion were 0.91 (95% CI: 0.48-0.99), 1.00 (95% CI: 0.87-1.00), 230.47 (95% CI: 6.79-7824.72), 0.09 (95% CI: 0.01-0.80), 2676.10 (95% CI: 61.35-120,000), and 1.00 (95% CI: 0.99-1.00), respectively. Subgroup analyses revealed that viscosity of contrast agent (P = 0.024) and test order (P = 0.036) affected the accuracy of MR-HSG to evaluate tubal occlusion.

Conclusions: Our meta-analysis indicated magnetic resonance hysterosalpingography may serve as an alternative for further evaluation of fallopian tubal occlusion of female infertility.
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http://dx.doi.org/10.1016/j.clinimag.2020.11.001DOI Listing
April 2021

Dosimetric comparison of tomotherapy and volumetric-modulated arc therapy for children with neuroblastoma.

Pediatr Investig 2020 Sep 27;4(3):186-191. Epub 2020 Sep 27.

Department of Radiotherapy Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing China.

Importance: Irradiation treatment for pediatric patients with neuroblastoma represents a major challenge due to the pediatric dose limits for critical structures and the necessity of sufficient dose coverage of the clinical target volume for local control.

Objective: To investigate dosimetric differences between tomotherapy (TOMO) and volumetric-modulated arc therapy (VMAT) as retroperitoneal radiotherapy for children with neuroblastoma.

Methods: Eight patients who received retroperitoneal radiotherapy for neuroblastoma were selected for comparison of TOMO and VMAT treatment plans. The D, D, D, D, D, and D of planning target volume (PTV), conformity index (CI), heterogeneity index (HI), and organs at risk (OARs) parameters were compared. Delivery machine unit (MU) and image-guide radiotherapy solution results were also compared.

Results: All patients received a cumulative dose of 19.5 Gy to the PTV. VMAT showed higher CI (0.93 ± 0.02), compared with TOMO (0.87 ± 0.03, 0.001). Notably, the average PTV HI was significantly better using TOMO (1.05 ± 0.01) than VMAT (1.08 ± 0.02, 0.003). Compared with VMAT, the D, D, and D all exhibited increases in TOMO; D variation was less than 1% in TOMO. The D for the spinal cord and D for the small intestine were better in TOMO in terms of OARs. However, TOMO had more MUs and required a longer delivery time.

Interpretation: Both planning techniques are capable of producing high- quality treatment plans. TOMO is superior for PTV coverage, but inferior for CI. TOMO requires extra treatment time; its cost is greater than the cost of VMAT.
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http://dx.doi.org/10.1002/ped4.12215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520106PMC
September 2020

An observational study on Ca supplementation and dietary intake during pregnancy on low birth weight and small for gestational age.

Public Health Nutr 2020 Nov 4:1-10. Epub 2020 Nov 4.

Department of Obstetrics and Gynecology, Gansu Provincial Maternity and Child Care Hospital, No.143, Qilihe North Street, Qilihe District, Lanzhou730050, Gansu Province, China.

Objective: To evaluate the effects of dietary Ca intake and Ca supplementation during pregnancy on low birth weight (LBW) and small for gestational age (SGA) infants.

Design: A birth cohort study was conducted in 2010-2012 at the Gansu Provincial Maternity and Child Care Hospital in Lanzhou, China.

Setting: A birth cohort study.

Participants: Totally, 9595 pregnant women who came to the hospital for delivery at 20 weeks of gestation or more, and who were 18 years of age or older.

Results: Compared with non-users, Ca supplement users had a reduced risk of LBW infants (OR = 0·77, 95 % CI: 0·63, 0·95) and a reduced risk of nulliparous women giving birth to LBW infants (OR = 0·75, 95 % CI: 0·58, 0·98) (P < 0·05). More specifically, both the use of Ca supplement before conception and during pregnancy (OR = 0·44, 95 % CI: 0·19, 0·99) and during pregnancy only (OR = 0·80, 95 % CI: 0·65, 0·99) had the main effect of reducing risk of nulliparous women giving birth to LBW infants (P < 0·05). There was no association between Ca supplementation and SGA (OR = 0·87, 95 % CI: 0·75, 1·01) (P > 0·05). However, higher dietary Ca intake during pregnancy decreases the risk of both LBW (quartile 2: OR = 0·72, 95 % CI: 0·55, 0·94; quartile 3: OR = 0·68, 95 % CI: 0·50, 0·62) and SGA infants (quartile 2: OR = 0·77, 95 % CI: 0·63, 0·95; quartile 3: OR = 0·71, 95 % CI: 0·57, 0·88, quartile 4: OR = 0·71, 95 % CI: 0·57, 0·88) (P < 0·05).

Conclusions: Ca supplementation and adequate dietary intake of Ca during pregnancy are associated with a decreased risk of LBW infants born to nulliparous women.
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http://dx.doi.org/10.1017/S1368980020004425DOI Listing
November 2020

Magnolol inhibits sodium currents in freshly isolated mouse dorsal root ganglion neurons.

Clin Exp Pharmacol Physiol 2021 03 27;48(3):347-354. Epub 2020 Oct 27.

Department of Clinical Pharmacy, China Pharmaceutical University, Nanjing, China.

The voltage-gated sodium channel (VGSC) currents in dorsal root ganglion (DRG) neurons contain mainly TTX-sensitive (TTX-S) and TTX-resistant (TTX-R) Na currents. Magnolol (Mag), a hydroxylated biphenyl compound isolated from the bark of Magnolia officinalis, has been well documented to exhibit analgesic effects, but its mechanism is not yet fully understood. The aim of the present study was to investigate whether the antinociceptive effects of Mag is through inhibition of Na currents. Na currents in freshly isolated mouse DRG neurons were recorded with the whole cell patch clamp technique. Results showed that Mag inhibited TTX-S and TTX-R Na currents in a concentration-dependent manner. The IC values for block of TTX-S and TTX-R Na currents were 9.4 and 7.0 μmol/L, respectively. Therefore, TTX-R Na current was more susceptible to Mag than TTX-S Na current. For TTX-S Na channel, 10 μmol/L Mag shifted the steady state inactivation curve toward more negative by 9.8 mV, without affecting the activation curve. For TTX-R Na channel, 7 μmol/L Mag shifted the steady state activation and inactivation curves toward more positive and negative potentials by 6.5 and 11.7 mV, respectively. In addition, Mag significantly postponed recovery of TTX-S and TTX-R Na currents from inactivation, and produced frequency dependent blocks of both subtypes of Na currents. These results suggest that the inhibitory effects of Mag on Na channels may contribute to its analgesic effect.
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http://dx.doi.org/10.1111/1440-1681.13422DOI Listing
March 2021

Development and validation of a deep learning algorithm for auto-delineation of clinical target volume and organs at risk in cervical cancer radiotherapy.

Radiother Oncol 2020 12 8;153:172-179. Epub 2020 Oct 8.

Department of Radiation Oncology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. Electronic address:

Purpose: The delineation of the clinical target volume (CTV) is a crucial, laborious and subjective step in cervical cancer radiotherapy. The aim of this study was to propose and evaluate a novel end-to-end convolutional neural network (CNN) for fully automatic and accurate CTV in cervical cancer.

Methods: A total of 237 computed tomography (CT) scans of patients with locally advanced cervical cancer were collected and evaluated. A novel 2.5D CNN network, called DpnUNet, was developed for CTV delineation and further applied for CTV and organ-at-risk (OAR) delineation simultaneously. Comprehensive comparisons and experiments were performed. The mean Dice similarity coefficient (DSC), 95th percentile Hausdorff distance (95HD) and subjective evaluation were used to assess the performance of this method.

Results: The mean DSC and 95HD values were 0.86 and 5.34 mm for the delineated CTVs. The clinical experts' subjective assessments showed that 90% of the predicted contours were acceptable for clinical usage. The mean DSC and 95HD values were 0.91 and 4.05 mm for bladder, 0.85 and 2.16 mm for bone marrow, 0.90 and 1.27 mm for left femoral head, 0.90 and 1.51 mm for right femoral head, 0.82 and 4.29 mm for rectum, 0.85 and 4.35 mm for bowel bag, 0.82 and 4.96 mm for spinal cord respectively. The average delineation time for one patient's CT images was within 15 seconds.

Conclusion: The experimental results demonstrate that the CTV and OARs delineated for cervical cancer by DpnUNet was in close agreement with the ground truth. DpnUNet could significantly reduce the radiation oncologists' contouring time.
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http://dx.doi.org/10.1016/j.radonc.2020.09.060DOI Listing
December 2020

Peptidome analysis of cerebrospinal fluid in neonates with hypoxic-ischemic brain damage.

Mol Brain 2020 10 2;13(1):133. Epub 2020 Oct 2.

Department of Newborn Infants, Children's Hospital of Nanjing Medical University, Nanjing, 210008, Jiangsu, China.

Hypoxic-ischemic brain injury (HIBD) causes neonatal death and serious neurological disability; however, there are currently no promising therapies for it excepting cooling. Therefore, in this study, we used peptidome analysis to identify differentially expressed peptides in cerebrospinal fluid (CSF) of neonates with HIBD or controls, which may give a foundation for finding new promising drugs of neonatal HIBD. CSF samples were collected from neonates with HIBD (n = 4) or controls (n = 4). ITRAQ LC-MS/MS was used to identify differentially expressed peptides between two groups. A total of 35 differentially expressed peptides from 25 precursor proteins were identified. The 2671.5 Da peptide (HSQFIGYPITLFVEKER), one of the down-regulated peptides in neonatal HIBD, is a fragment of heat shock protein 90-alpha (HSP90α/HSP90AA1). Results of bioinformatics analysis showed that HSP90α/HSP90AA1 was located in the protein-protein interaction (PPI) network hub and was involved in the NOD-LIKE receptor (NLR) signaling pathway. This peptide, we named it Hypoxic-Ischemic Brain Damage Associated Peptide (HIBDAP), is a hydrophilic peptide with high stability and has a long half-life of 3.5 h in mammalian reticulocytes. It was demonstrated that TAT-HIBDAP could successfully enter PC12 cells and further into the nucleus. After HIBDAP pretreatment and 6 h of OGD treatment, low concentrations of HIBDAP increased the survival rate of cells, except 40 μM had a toxic effect. Safe concentrations of HIBDAP reduced pyroptosis of PC12 cells under OGD, except 20 μM had no effect, by suppressing expressions of NLRP3, ASC and Caspase-1 except NLRP1. The results of our study identified the characterization and expression profiles of peptides in CSF of neonatal HIBD. Several meaningful peptides such as HIBDAP may play significant roles in neonatal HIBD and provide new therapeutic targets for neonatal HIBD.
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http://dx.doi.org/10.1186/s13041-020-00671-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7531121PMC
October 2020

Effects of Nasal Continuous Positive Airway Pressure on Cerebral Hemodynamics in Preterm Infants.

Front Pediatr 2020 21;8:487. Epub 2020 Aug 21.

Department of Newborn Infants, Children's Hospital of Nanjing Medical University, Nanjing, China.

To evaluate the effects of pressure levels on cerebral hemodynamics in premature infants receiving nasal continuous positive airway pressure (nCPAP) during the first 3 days of life. Forty-four preterm infants treated with nCPAP were divided into two groups: very preterm infants [gestational age 1 (GA1), GA < 32 weeks, = 24] and moderate/late preterm infants (GA2 group, GA 32-37 weeks, = 20). During monitoring, pressure levels were set at 4 → 6 → 8 → 4 cmHO, and cerebral hemodynamics was assessed by near-infrared spectroscopy (NIRS). Vital signs, peripheral oxygen saturation (SpO) and transcutaneous carbon dioxide pressure (TcPCO) were simultaneously recorded. Pressures of 4-8 cmHO had no significant influence on cerebral hemodynamics, TcPCO, SpO or other vital signs. The tissue oxygenation index (TOI), the difference between oxygenated hemoglobin (ΔHbO) and deoxygenated hemoglobin (ΔHHb) (ΔHbD), and cerebral blood volume (ΔCBV) were all significantly positively correlated with gestational and post-natal age, with fluctuations being greater in the GA1 group. ΔHbD and ΔCBV were also significantly positively correlated with TcPCO. No significant differences were observed in cerebral hemodynamics when the nCPAP pressure was set to 4-8 cmHO.
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http://dx.doi.org/10.3389/fped.2020.00487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472537PMC
August 2020

Alternatively Spliced Isoforms Play Opposing Roles in Anthocyanin Biosynthesis of L.

Front Plant Sci 2020 19;11:983. Epub 2020 Aug 19.

National Key Laboratory of Crop Genetic Improvement, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.

L. (rapeseed, oilseed rape, and canola) and varieties of its two diploid parents, and , display a large amount of variation in anthocyanin pigmentation of the leaf, stem, and fruit. Here, we demonstrate that , an ortholog of the anthocyanin activator that confers purple traits, positively regulates anthocyanin biosynthesis in leaves of . Sequencing of and transgenic analysis suggests that activation of this gene in purple rapeseed may result from a single nucleotide and/or 2bp insertion in its promoter region. gives rise to three splice variants, designated , and according to the length of the transcripts. While encodes a full-length R2R3-MYB, both and encode truncated proteins that lack both a partial R3 repeat and the complete C terminal domain, and so are unable to interact with the bHLH protein AtTT8. Although expression of either or in green rapeseed does not result in purple leaves, both genes do modify genome-wide gene expression, with a strong repression of anthocyanin-related genes. We have demonstrated that regulates anthocyanin accumulation in leaves of and propose a potential mechanism for modulation of anthocyanin biosynthesis by alternative splicing.
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http://dx.doi.org/10.3389/fpls.2020.00983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466728PMC
August 2020
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