Publications by authors named "Jie Qin"

367 Publications

TMT-Based Quantitative Proteomic Analysis Reveals the Effect of Bone Marrow Derived Mesenchymal Stem Cell on Hair Follicle Regeneration.

Front Pharmacol 2021 14;12:658040. Epub 2021 Jun 14.

Department of Dermatology, The First Hospital of China Medical University, Shenyang, China.

Hair loss (HL) is a common chronic problem of poorly defined etiology. Herein, we explored the functionality of bone marrow-derived mesenchymal stem cell (BMSC) and conditioned medium (MSC-CM) as regulators of hair follicle proliferation and regeneration, and the mechanistic basis for such activity. BMSC were cultured and identified through the induction of multilineage differentiation and the use of a CCK-8 kit. The dorsal skin of mice was then injected with BMSC and MSC-CM, and the impact of these injections on hair cycle transition and hair follicle stem cell (HFSC) proliferation was then evaluated via hematoxylin and eosin (H&E) staining and immunofluorescent (IF) staining. We then conducted a tandem mass tags (TMT)-based quantitative proteomic analysis of control mice and mice treated with BMSC or MSC-CM to identify differentially expressed proteins (DEPs) associated with these treatments. Parallel reaction monitoring (PRM) was utilized as a means of verifying our proteomic analysis results. Herein, we found that BMSC and MSC-CM injection resulted in the transition of telogen hair follicles to anagen hair follicles, and we observed the enhanced proliferation of HFSCs positive for Krt15 and Sox9. Our TMT analyses identified 1,060 and 770 DEPs (fold change>1.2 or<0.83 and < 0.05) when comparing the BMSC vs. control and MSC-CM vs. control groups, respectively. Subsequent PRM validation of 14 selected DEPs confirmed these findings, and led to the identification of Stmn1, Ncapd2, Krt25, and Ctps1 as hub DEPs in a protein-protein interaction network. Together, these data suggest that BMSC and MSC-CM treatment can promote the proliferation of HFSCs, thereby facilitating hair follicle regeneration. Our proteomics analyses further indicate that Krt25, Cpm, Stmn1, and Mb may play central roles in hair follicle transition in this context and may represent viable clinical targets for the treatment of HL.
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http://dx.doi.org/10.3389/fphar.2021.658040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237093PMC
June 2021

LINGO-1 regulates Wnt5a signaling during neural stem and progenitor cell differentiation by modulating miR-15b-3p levels.

Stem Cell Res Ther 2021 06 29;12(1):372. Epub 2021 Jun 29.

Department of Rehabilitation Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

Background: Manipulation of neural stem and progenitor cells (NSPCs) is critical for the successful treatment of spinal cord injury (SCI) by NSPC transplantation, since their differentiation into neurons and oligodendrocytes can be inhibited by factors present in inflamed myelin. In this study, we examined the effects of LINGO-1 on spinal cord-derived NSPC (sp-NSPC) differentiation, the underlying mechanisms of action, and the functional recovery of mice after transplantation of manipulated cells.

Methods: sp-NSPCs were harvested from female adult C57/BL6 mice after SCI induced with an NYU impactor. These cells were infected with lentiviral vectors containing LINGO-1 shRNA sequence or a scrambled control and transplanted into SCI mice. Tuj-1- and GFAP-positive cells were assessed by immunofluorescence staining. Wnt5a, p-JNK, JNK, and β-catenin expression was determined by Western blot and RT-qPCR. miRNAs were sequenced to detect changes in miRNA expression. Motor function was evaluated 0-35 days post-surgery by means of the Basso Mouse Scale (BMS) and by the rotarod performance test.

Results: We discovered that LINGO-1 shRNA increased neuronal differentiation of sp-NSPCs while decreasing astrocyte differentiation. These effects were accompanied by elevated Wnt5a protein expression, but unexpectedly, no changes in Wnt5a mRNA levels. miRNA-sequence analysis demonstrated that miR-15b-3p was a downstream mediator of LINGO-1 which suppressed Wnt5a expression. Transplantation of LINGO-1 shRNA-treated sp-NSPCs into SCI mice promoted neural differentiation, wound compaction, and motor function recovery.

Conclusions: LINGO-1 shRNA promotes neural differentiation of sp-NSPCs and Wnt5a expression, probably by downregulating miR-15b-3p. Transplantation of LINGO-1 shRNA-treated NSPCs promotes recovery of motor function after SCI, highlighting its potential as a target for SCI treatment.
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http://dx.doi.org/10.1186/s13287-021-02452-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243903PMC
June 2021

Roles of Long Noncoding RNAs in Conferring Glioma Progression and Treatment.

Front Oncol 2021 11;11:688027. Epub 2021 Jun 11.

Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Accompanying the development of biomedicine, our knowledge of glioma, one of the most common primary intracranial carcinomas, is becoming more comprehensive. Unfortunately, patients with glioblastoma (GBM) still have a dismal prognosis and a high relapse rate, even with standard combination therapy, namely, surgical resection, postoperative radiotherapy and chemotherapy. The absence of validated biomarkers is responsible for the majority of these poor outcomes, and reliable therapeutic targets are indispensable for improving the prognosis of patients suffering from gliomas. Identification of both precise diagnostic and accurate prognostic markers and promising therapeutic targets has therefore attracted considerable attention from researchers. Encouragingly, accumulating evidence has demonstrated that long noncoding RNAs (lncRNAs) play important roles in the pathogenesis and oncogenesis of various categories of human tumors, including gliomas. Nevertheless, the underlying mechanisms by which lncRNAs regulate diverse biological behaviors of glioma cells, such as proliferation, invasion and migration, remain poorly understood. Consequently, this review builds on previous studies to further summarize the progress in the field of lncRNA regulation of gliomas over recent years and addresses the potential of lncRNAs as diagnostic and prognostic markers and therapeutic targets.
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http://dx.doi.org/10.3389/fonc.2021.688027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226164PMC
June 2021

Clinical characteristics of Kawasaki disease and concurrent pathogens during isolation in COVID-19 pandemic.

World J Pediatr 2021 06 23;17(3):263-271. Epub 2021 Jun 23.

Cardiology Department, Children's Hospital of Soochow University, Suzhou, 215025, China.

Background: The aim of this study is to explore the characteristics of Kawasaki disease (KD) and concurrent pathogens due to a stay-at-home isolation policy during coronavirus disease 2019 (COVID-19) epidemic.

Methods: All patients with KD admitted between February and April in 2015-2020, were classified into before (group 1, in 2015-2019) and after (group 2, in 2020) isolation groups. A total of 4742 patients [with KD (n = 98) and non-KD (n = 4644)] referred to Mycoplasma pneumoniae (MP) and virus detection were analyzed in 2020. Clinical characteristics, laboratory data, and 13 pathogens were analyzed retrospectively.

Results: Group 2 had a significantly increased incidence of KD (0.11%) with 107 patients compared to that of group 1 (0.03%) with 493 patients. The comparisons of oral mucosal change, strawberry tongue, desquamation of the fingertips, cervical lymphadenopathy and neutrophil percentage decreased in group 2 compared to group 1. The infection rate of MP increased significantly in group 2 (34.7%) compared to group 1 (19.3%), while the positive rate of viruses decreased significantly in group 2 (5.3%) compared to group 1 (14.3%). In 2020, the positive rate of MP infection increased significantly in patients with KD compared to the increase in patients with non-KD. The infection rate of MP for younger children aged less than 3 years old was higher in group 2 than in group 1.

Conclusion: Compared with the characteristics of KD from 2015 to 2019 years, the incidence of KD was increased in 2020 and was accompanied by a high incidence of MP infection, especially in younger children (less than 3 years old) during the isolation due to COVID-19 pandemic.
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http://dx.doi.org/10.1007/s12519-021-00431-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219783PMC
June 2021

High prevalence of osteoporosis in patients undergoing spine surgery in China.

BMC Geriatr 2021 06 13;21(1):361. Epub 2021 Jun 13.

Department of Spine Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Background: With the increase in life expectancy, a large number of patients with osteoporosis (OP) are undergoing spine surgery, which may adversely affect the surgical success rate. The prevalence of OP varies in different regions, and no data are available that represent the prevalence of OP among Chinese patients over 50 years of age who are undergoing spine surgery. It was the first multicenter study to assess OP in these patients. Aiming to obtain comprehensive data, this study combined bone mineral density (BMD) measurements and visual radiography assessment (VRA) to analyze the prevalence of OP in patients aged > 50 years who underwent spine surgery.

Methods: Data from 1,856 patients aged over 50 years undergoing spine surgery who resided in northern, central, and southern China were reviewed between 2018 and 2019. Based on the perioperative BMD and X-ray data, we calculated the prevalence of OP in this special population according to sex, age, and spine degenerative disease.

Results: A total of 1,245 patients (678 females and 567 males) were included in the study. The prevalence of OP diagnosed by BMD was 52.8 % in females and 18.7 % in males. When we combined with BMD and VRA, the prevalence of OP increased from 52.8 to 65.9 % in females and from 18.7 to 40.6 % in males. Although OP was more severe in females than in males, a significant difference in the rate of vertebral fracture (VF) was not observed between females and males with a normal BMD and osteopenia (females vs. males: aged 50-59 years, P = 0.977; 60-69 years, P = 0.302; >70 years, P = 0.172). Similarly, no significant difference in the vertebral fracture rate was observed within different age groups of patients with a normal BMD and osteopenia (females: P = 0.210; males, P = 0.895). The incidence of OP in patients with degenerative scoliosis was higher than that in the remaining patients (females: 63.6 % vs. 42.4 %, P = 0.018; males: 38.9 % vs. 13.8 %, P = 0.004).

Conclusions: A high prevalence of OP was identified in patients aged > 50 years undergoing spine surgery, especially in patients whose primary diagnosis was degenerative scoliosis. BMD and VRA evaluations should be included in the clinical routine for these patients prior to surgery.
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http://dx.doi.org/10.1186/s12877-021-02313-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201731PMC
June 2021

Revisiting stacking interactions in tetrathiafulvalene and selected derivatives using tight-binding quantum chemical calculations and local coupled-cluster method.

Acta Crystallogr B Struct Sci Cryst Eng Mater 2021 Jun 13;77(Pt 3):311-320. Epub 2021 May 13.

School of Life Sciences, Shandong University of Technology, 266 Xincun West Road, Zibo, Shandong 255049, People's Republic of China.

The engineering of supramolecular architectures needs accurate descriptions of the intermolecular interactions in crystal structures. Tetrathiafulvalene (TTF) is an effective building block used in the construction of promising functional materials. The parallel packing of the neutral TTF-TTF system was studied previously using the high-level quantum chemical method, advancing it as a valuable model system. The recently developed tight-binding quantum chemical method GFN2-xTB and local coupled-cluster method DLPNO-CCSD(T) were used to investigate the stacking interactions of TTF and selected derivatives deposited in the Cambridge Structural Database. Using the interaction energy of the TTF-TTF dimer calculated at the CCSD(T)/CBS level as the reference, the accuracies of the two methods are investigated. The energy decomposition analysis within the DLPNO-CCSD(T) framework reveals the importance of dispersion interaction in the TTF-related stacking systems. The dispersion interaction density plot vividly shows the magnitude and distribution of the dispersion interaction, providing a revealing insight into the stacking interactions in crystal structures. The results show that the GFN2-xTB and DLPNO-CCSD(T) methods could achieve accuracy at an affordable computational cost, which would be valuable in understanding the nature of parallel stacking in supramolecular systems.
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http://dx.doi.org/10.1107/S2052520621003085DOI Listing
June 2021

Ubiquitin-Specific Protease 14 (USP14) Aggravates Inflammatory Response and Apoptosis of Lung Epithelial Cells in Pneumonia by Modulating Poly (ADP-Ribose) Polymerase-1 (PARP-1).

Inflammation 2021 Jun 3. Epub 2021 Jun 3.

Department of Pediatrics, Yijishan Hospital, Wannan Medical College, No. 2, West Road of Zheshan, Jinghu District, Anhui, 241001, Wuhu, China.

Pneumonia is one of the common respiratory diseases in pediatrics. Ubiquitin-specific protease 14 (USP14) contributes the progress of inflammation-associated diseases. Poly (ADP-ribose) polymerase-1 (PARP-1) involves in the signal transduction of inflammatory pulmonary disease. This study aims to identify the precise function and elaborate the regulatory mechanism of USP14/PARP-1 in the injury of lung epithelial cells. Human lung epithelial BEAS-2B cells received lipopolysaccharide (LPS) (0, 1, 5, and 10 mg/L) treatment for 16 h, establishing in vitro pneumonia model. USP14 protein and mRNA levels in LPS-injured lung epithelial cells were separately assessed using western blot and RT-qPCR analysis. Lung epithelial cells were transfected with siRNA-USP14 or OV-USP14 to perform gain- or loss-of-function experiments. CCK-8 assay was applied to assess cell viability. TUNEL staining and western blot analysis were adopted to determine cell apoptosis. In addition, release of inflammatory cytokines and nitric oxide (NO) was detected using the commercial kits. Meanwhile, PARP-1 protein levels in LPS-injured lung epithelial cells were detected by performing western blot assay. Moreover, Co-IP assay was utilized for detection of the interaction between USP14 and PARP-1. The regulatory effects of PARP-1 on USP14 function in LPS-injured lung epithelial cells were also investigated. LPS dose-dependently reduced viability of lung epithelial cells and elevated USP14 protein. USP14 combined with PARP-1 and increased PARP-1 expression. USP14 elevation exacerbated inflammatory injury and boosted the apoptosis of LPS-injured lung epithelial cells, which was reversed upon downregulation of PARP-1. To sum up, USP14 promotion exacerbated inflammatory injury and boosted the apoptosis of LPS-injured lung epithelial cells by upregulating PARP-1 expression. These findings may represent a therapeutic target for clinical intervention in pneumonia.
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http://dx.doi.org/10.1007/s10753-021-01482-3DOI Listing
June 2021

An Indoor Localization System Using Residual Learning with Channel State Information.

Entropy (Basel) 2021 May 7;23(5). Epub 2021 May 7.

College of Electronic and Optical Engineering, Nanjing University of Posts and Telecommunications, Nanjing 210023, China.

With the increasing demand of location-based services, neural network (NN)-based intelligent indoor localization has attracted great interest due to its high localization accuracy. However, deep NNs are usually affected by degradation and gradient vanishing. To fill this gap, we propose a novel indoor localization system, including denoising NN and residual network (ResNet), to predict the location of moving object by the channel state information (CSI). In the ResNet, to prevent overfitting, we replace all the residual blocks by the stochastic residual blocks. Specially, we explore the long-range stochastic shortcut connection (LRSSC) to solve the degradation problem and gradient vanishing. To obtain a large receptive field without losing information, we leverage the dilated convolution at the rear of the ResNet. Experimental results are presented to confirm that our system outperforms state-of-the-art methods in a representative indoor environment.
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http://dx.doi.org/10.3390/e23050574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151952PMC
May 2021

Bioinformatics identification of hub genes and signaling pathways regulated by intravenous immunoglobulin treatment in acute Kawasaki disease.

Exp Ther Med 2021 Jul 19;22(1):784. Epub 2021 May 19.

Institute of Pediatric Research, Children's Hospital of Soochow University, Suzhou, Jiangsu 215025, P.R. China.

Kawasaki disease (KD) is an acute, self-limiting form of vasculitis commonly encountered in infants and young children. Intravenous immunoglobulin (IVIG) is the primary drug used for the treatment of KD, which may significantly reduce the occurrence of coronary artery lesions. However, the specific molecular profile changes of KD caused by IVIG treatment have remained elusive and require further research. The present study was designed to identify key genes, pathways and immune cells affected by IVIG treatment using multiple bioinformatics analysis methods. The results suggested that myeloid cells and neutrophils were affected by IVIG treatment. Kyoto Encyclopedia of Genes and Genomes pathway analysis identified that hematopoietic cell lineages and osteoclast differentiation may have an important role in the mechanism of action of IVIG treatment. Immune cell analysis indicated that the levels of monocytes, M1 macrophages, neutrophils and platelets were markedly changed in patients with KD after vs. prior to IVIG treatment. The key upregulated genes, including ZW10 interacting kinetochore protein, GINS complex subunit 1 and microRNA-30b-3p in whole blood cells of patients with KD following treatment with IVIG indicated that these IVIG-targeted molecules may have important roles in KD. In addition, these genes were further examined by literature review and indicated to be involved in cell proliferation, apoptosis and virus-related immune response in patients with KD. Therefore, the present results may provide novel insight into the mechanisms of action of IVIG treatment for KD.
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http://dx.doi.org/10.3892/etm.2021.10216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145699PMC
July 2021

Confocal Raman microspectral analysis and imaging of the drug response of osteosarcoma to cisplatin.

Anal Methods 2021 06;13(22):2527-2536

State Key Laboratory of Photon-Technology in Western China Energy, Institute of Photonics and Photon-Technology, Northwest University, #1 Xuefu Avenue, Guodu Education and Technology Industrial Zone Chang'an District, Xi'an, Shaanxi 710127, China.

Confocal Raman microspectral analysis and imaging were used to elucidate the drug response of osteosarcoma (OS) to cisplatin. Raman spectral data were obtained from OS cells that were untreated (UT group) and treated with 20 µM (20T group) and 40 µM (40T group) cisplatin for 24 hours. Statistical analysis of the changes in specific Raman signals was performed using a one-way ANOVA and multiple Tukey's honest significant difference (HSD) post hoc tests. Principal component analysis-linear discriminant analysis (PCA-LDA) was used to highlight the featured cellular drug responses based on the obtained spectral information. For spectral imaging analysis, k-means cluster analysis (KCA) was adopted to clarify the effect of cisplatin dose changes on the subcellular structure and its biochemical composition. The results suggest that the major biochemical changes induced by cisplatin in OS cells undergoing apoptosis are reduced protein and nucleic acid content. Through univariate analysis, the changes in the distribution of nucleic acids in OS cells induced by different doses of cisplatin were obtained. The combination of Raman spectroscopy and multivariate analysis shows that cisplatin mainly acts on the nucleus and causes changes in the secondary structure of proteins. These results indicate that Raman imaging technology has the potential to offer the basis of dose optimization for personalized cancer treatment by helping to understand in vitro cellular drug interactions.
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http://dx.doi.org/10.1039/d1ay00626fDOI Listing
June 2021

A Generalized Method for Binary Optimization: Convergence Analysis and Applications.

IEEE Trans Pattern Anal Mach Intell 2021 Apr 2;PP. Epub 2021 Apr 2.

Binary optimization problems (BOPs) arise naturally in many fields, such as information retrieval, computer vision, and machine learning. Most existing binary optimization methods either use continuous relaxation which can cause large quantization errors, or incorporate a highly specific algorithm that can only be used for particular loss functions. To overcome these difficulties, we propose a novel generalized optimization method, named Alternating Binary Matrix Optimization (ABMO), for solving BOPs. ABMO can handle BOPs with/without orthogonality or linear constraints for a large class of loss functions. ABMO involves rewriting the binary, orthogonality and linear constraints for BOPs as an intersection of two closed sets, then iteratively dividing the original problems into several small optimization problems that can be solved as closed forms. To provide a strict theoretical convergence analysis, we add a sufficiently small perturbation and translate the original problem to an approximated problem whose feasible set is continuous. We not only provide rigorous mathematical proof for the convergence to a stationary and feasible point, but also derive the convergence rate of the proposed algorithm. The promising results obtained from four binary optimization tasks validate the superiority and the generality of ABMO compared with the state-of-the-art methods.
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http://dx.doi.org/10.1109/TPAMI.2021.3070753DOI Listing
April 2021

Diagnosis of Retrolingual Obstruction during Drug-Induced Sleep Endoscopy versus Polysomnography with Nasopharyngeal Tube in Patients with Obstructive Sleep Apnea.

Ann Otol Rhinol Laryngol 2021 Mar 29:34894211005944. Epub 2021 Mar 29.

Department of Otolaryngology, General Hospital of Northern Theater Command, Shenyang, China.

Objective: To compare the retrolingual obstruction during drug-induced sleep endoscopy (DISE) with the retrolingual obstruction during polysomnography with nasopharyngeal tube (NPT-PSG).

Methods: A cross-sectional study of 77 consecutive patients with moderate and severe obstructive sleep apnea (OSA) was conducted. After 15 patients were excluded from the study for not completing DISE or NPT-PSG successfully, 62 patients were included in this study. Retrolingual sites of obstruction grade 2 determined by DISE according to the VOTE (velum, oropharynx lateral wall, tongue base, and epiglottis) classification were considered as retrolingual obstruction, while apnea-hypopnea index (AHI) ≥ 15 events/hour determined by NPT-PSG was considered as retrolingual obstruction. The extent of agreement between DISE and NPT-PSG findings was evaluated using unweighted Cohen's kappa test.

Results: The 62 study participants (11 moderate OSA, 51 severe OSA) had a mean (SD) age of 39.8 (9.9) years, and 58 (94%) were men. No statistically significant differences between included and excluded patients were observed in patient characteristics. The extent of agreement in retrolingual obstruction between DISE and NPT-PSG was 80.6% (Cohen  = 0.612; 95% CI, 0.415-0.807).

Conclusion: Retrolingual obstruction requiring treatment showed good agreement between DISE and NPT-PSG, suggesting that NPT-PSG may also be a reliable method to assess the retrolingual obstruction.
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http://dx.doi.org/10.1177/00034894211005944DOI Listing
March 2021

Development and Validation of Retinal Vasculature Nomogram in Suspected Angina Due to Coronary Artery Disease.

J Atheroscler Thromb 2021 Mar 19. Epub 2021 Mar 19.

Guangdong Eye Institute, Department of Ophthalmology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences.

Aims: To develop and validate a nomogram using retinal vasculature features and clinical variables to predict coronary artery disease (CAD) in patients with suspected angina.

Methods: The prediction model consisting of 795 participants was developed in a training set of 508 participants with suspected angina due to CAD, and data were collected from January 2018 to June 2019. The held-out validation was conducted with 287 consecutive patients from July 2019 to November 2019. All patients with suspected CAD received optical coherence tomography angiography (OCTA) examination before undergoing coronary CT angiography. LASSO regression model was used for data reduction and feature selection. Multivariable logistic regression analysis was used to develop the retinal vasculature model for predicting the probability of the presence of CAD.

Results: Three potential OCTA parameters including vessel density of the nasal and temporal perifovea in the superficial capillary plexus and vessel density of the inferior parafovea in the deep capillary plexus were further selected as independent retinal vasculature predictors. Model clinical electrocardiogram (ECG) OCTA (clinical variables+ECG+OCTA) was presented as the individual prediction nomogram, with good discrimination (AUC of 0.942 [95% CI, 0.923-0.961] and 0.897 [95% CI, 0.861-0.933] in the training and held-out validation sets, respectively) and good calibration. Decision curve analysis indicated the clinical applicability of this retinal vasculature nomogram.

Conclusions: The presented retinal vasculature nomogram based on individual probability can accurately identify the presence of CAD, which could improve patient selection and diagnostic yield of aggressive testing before determining a diagnosis.
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http://dx.doi.org/10.5551/jat.62059DOI Listing
March 2021

Nitrogen Deposition Reduces the Diversity and Abundance of Gene-Containing CO-Fixing Microorganisms in the Soil of the Steppe.

Front Microbiol 2021 2;12:570908. Epub 2021 Mar 2.

Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin, China.

CO fixation by autotrophic microbes has a significant effect on the carbon cycle in temperate grasslands. Nitrogen (N) deposition in soil has been steadily increasing for decades, which has consequences for soil microorganisms. However, the impact of this deposition on the diversity and abundance of CO-fixing soil microorganisms remains unclear in temperate grasslands. In the present study, the gene, a key gene in the Calvin-Benson-Bassham cycle that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase, was used to study CO-fixing microbes under different rates of N addition (0, 15, 30, 50, 100, and 150 kg N ha yr) in a 9-year field experiment in a temperate grassland. The results showed that N addition led to significant reductions in gene abundance and genetic diversity and altered gene community composition. High N addition enhanced the relative abundances of Acidiferrobacterales and Rhizobiales but reduced those of Burkholderiales and Rhodobacterales. Structural equation modeling further revealed that N addition primarily reduced genetic diversity by increasing the soil NO-N content and decreasing the soil pH. N addition indirectly reduced gene abundance, possibly by increasing the soil N/phosphorus (P) ratio and decreasing the soil pH. These findings suggest that N addition increases the soil available N and causes soil acidification, which may inhibit growth of CO-fixing microbes to some extent.
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http://dx.doi.org/10.3389/fmicb.2021.570908DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961154PMC
March 2021

Leukocyte proteomics coupled with serum metabolomics identifies novel biomarkers and abnormal amino acid metabolism in Kawasaki disease.

J Proteomics 2021 05 15;239:104183. Epub 2021 Mar 15.

Department of Cardiology, Children's Hospital of Soochow University, Suzhou 215025, China. Electronic address:

Kawasaki disease (KD) is a systemic vasculitis that can lead to severe cardiovascular complications, whereas the development and clinical usage of specific biomarkers might help diagnose KD and avoid certain complications. To this end, the molecular profiles of acute KD patients with coronary artery lesions (CAL) were first investigated through leukocyte proteomics and serum metabolomics assays. A total of 269 differentially abundant proteins and 35 differentially abundant metabolites with the top fold-changed levels were identified in acute KD patients compared to those in the healthy controls. Among them, several highly promising candidate marker proteins and metabolites indicative of KD progression were further analysed, such as the increased proteins ALPL, NAMPT, and S100P, as well as the decreased proteins C1QB and apolipoprotein family members. Moreover, metabolites, including succinic acid, dGMP, hyaluronic acid, L-tryptophan, propionylcarnitine, inosine, and phosphorylcholine, were found to be highly accurate at distinguishing between KD patients and healthy controls. Interestingly, the abnormal expression levels of a distinct set of proteins and metabolites in acute KD patients can be restored to normal levels upon intravenous immunoglobulin (IVIG) treatment. Overall, this work has revealed novel biomarkers and abnormal amino-acid metabolism as a prominent feature involved in KD patients with CAL. SIGNIFICANCE: KD is frequently concomitant with the development of life-threatening coronary vasculitis. Here, the profiles of leukocyte proteomics and serum metabolomics in acute KD patients with CALs were first investigated, and several hub molecules identified here could be used as supplemental biomarkers for KD diagnosis. Moreover, the metabolomic abnormalities especially the amino acids are particularly prominent in KD patients. Interestingly, the abnormal expression levels of a distinct set of proteins and metabolites in acute KD patients can be restored to normal levels upon IVIG treatment. Therefore, these findings might help understand the IVIG activities and also the underlying mechanisms of IVIG-resistant patients, thereby providing a new perspective for the exploration of mechanisms related to KD.
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http://dx.doi.org/10.1016/j.jprot.2021.104183DOI Listing
May 2021

The Added Value of Inflow-Based Vascular-Space-Occupancy and Diffusion-Weighted Imaging in Preoperative Grading of Gliomas.

Neurodegener Dis 2020 18;20(4):123-130. Epub 2021 Mar 18.

Department of Medical Imaging, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Objectives: The present study aimed to study whether combined inflow-based vascular-space-occupancy (iVASO) MR imaging (MRI) and diffusion-weighted imaging (DWI) improve the diagnostic accuracy in the preoperative grading of gliomas.

Methods: Fifty-one patients with histopathologically confirmed diffuse gliomas underwent preoperative structural MRI, iVASO, and DWI. We performed 2 qualitative consensus reviews: (1) structural MR images alone and (2) structural MR images with iVASO and DWI. Relative arteriolar cerebral blood volume (rCBVa) and minimum apparent diffusion coefficient (mADC) were compared between low-grade and high-grade gliomas. Receiver operating characteristic (ROC) curve analysis was performed to compare the tumor grading efficiency of rCBVa, mADC, and the combination of the two parameters.

Results: Two observers diagnosed accurate tumor grade in 40 of 51 (78.4%) patients in the first review and in 46 of 51 (90.2%) in the second review. Both rCBVa and mADC showed significant differences between low-grade and high-grade gliomas. ROC analysis gave a threshold value of 1.52 for rCBVa and 0.85 × 10-3 mm2/s for mADC to provide a sensitivity and specificity of 88.0 and 81.2% and 100.0 and 68.7%, respectively. The area under the ROC curve (AUC) was 0.87 and 0.85 for rCBVa and mADC, respectively. The combination of rCBVa and mADC values increased the AUC to 0.92.

Conclusion: The combined application of iVASO and DWI may improve the diagnostic accuracy of glioma grading.
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http://dx.doi.org/10.1159/000512545DOI Listing
March 2021

SICD: Novel Single-Access-Point Indoor Localization Based on CSI-MIMO with Dimensionality Reduction.

Sensors (Basel) 2021 Feb 13;21(4). Epub 2021 Feb 13.

College of Electronic and Optical Engineering, Nanjing University of Posts and Telecommunications, Nanjing 210023, China.

With the rise of location-based services and the rapidly growing requirements related to their applications, indoor localization based on channel state information-multiple-input multiple-output (CSI-MIMO) has become an important research topic. However, indoor localization based on CSI-MIMO has some disadvantages, including noise and high data dimensions. To overcome the above drawbacks, we proposed a novel method of indoor localization based on CSI-MIMO, named SICD. For SICD, a novel localization fingerprint was first designed which can reflect the time-frequency and space-frequency characteristics of CSI-MIMO under a single access point (AP). To reduce the redundancy in the data of CSI-MIMO amplitude, we developed a data dimensionality reduction algorithm. Moreover, by leveraging a log-normal distribution, we calculated the conditional probability of the naive Bayes classifier, which was used to predict the moving object's location. Compared with other state-of-the-art methods, the results of the experiment confirm that the SICD effectively improves localization accuracy.
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http://dx.doi.org/10.3390/s21041325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918435PMC
February 2021

Value of peripheral blood neutrophil-to-lymphocyte ratio for clinical diagnosis and prognosis of elderly patients with chronic heart failure and atrial fibrillation.

Cardiovasc J Afr 2021 Mar 2;32:1-4. Epub 2021 Mar 2.

Department of Laboratory, Xingtai Medical College, Xingtai 054000, Hebei Province, PR China. Email:

Aim: We aimed to explore the value of peripheral blood neutrophil-to-lymphocyte ratio (NLR) for the clinical diagnosis and prognosis of elderly patients with chronic heart failure (CHF) and atrial fibrillation (AF).

Methods: A total of 248 eligible patients were followed up for five years, and divided into major adverse cardiovascular event (MACE) and non-MACE groups. The independent predictive factors for MACE were explored by multivariate logistic regression analysis. Based on quartile of NLR, they were divided into groups A to D. The duration of MACE was analysed using Kaplan-Meier survival curves. The diagnostic value of NLR for MACE was evaluated by receiver operating characteristic curves.

Results: Higher age, low-density lipoprotein cholesterol and NLR, lower left ventricular ejection fraction, diabetes and NYHA heart function class III and IV were independent predictive factors for MACE. The incidence of MACE rose with increasing NLR. Groups A to D had significantly different rates of acute myocardial infarction, severe arrhythmia and cardiac death ( < 0.05). The average duration of MACE in groups A to D were 49.31, 45.27, 43.63 and 40.34 months, respectively.

Conclusions: The sensitivity and specificity of NLR for diagnosis of MACE were 72.39 and 86.18%, respectively. NLR was an independent predictive factor for MACE in these elderly patients with CHF and AF.
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http://dx.doi.org/10.5830/CVJA-2021-004DOI Listing
March 2021

Changes in physicochemical properties and microfauna community during vermicomposting of municipal sludge under different moisture conditions.

Environ Sci Pollut Res Int 2021 Jun 19;28(24):31539-31548. Epub 2021 Feb 19.

State Key Laboratory of Pollution Control and Source Reuse, Tongji University, Shanghai, 200092, China.

The present study aimed to explore the effect of a range of moisture content levels, including 65%, 72%, and 78%, on physicochemical properties and microfauna communities during vermicomposting of municipal sludge. As a result, death of perishable microfauna together with the degradation of organic matter was the dominant response in all groups in the early period of vermicomposting, while the effects of moisture content levels on various physiochemical parameters did not appear until the mid-later period. After the treatment with 78% moisture content, the content of mineral nitrogen was 1.186 g/kg in the sludge, with a 9.36 × 10 ind./g of microfauna quantity and 663.01 g of earthworm biomass. The values of these three measurements in 78% group were significantly higher than other two groups (p < 0.05), indicating that the effects of 78% moisture content were more pronounced for promoting nitrogen mineralization as well as microfauna and earthworms growth during vermicomposting. Specifically, testate amoebae were strongly associated with nitrification process, while nematodes were related to ammonification and phosphorus mineralization, of which testate amoebae had great potential of being bioindicators during vermicomposting of municipal sludge.
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http://dx.doi.org/10.1007/s11356-021-12846-5DOI Listing
June 2021

Semantics-Aware Spatial-Temporal Binaries for Cross-Modal Video Retrieval.

IEEE Trans Image Process 2021 18;30:2989-3004. Epub 2021 Feb 18.

With the current exponential growth of video-based social networks, video retrieval using natural language is receiving ever-increasing attention. Most existing approaches tackle this task by extracting individual frame-level spatial features to represent the whole video, while ignoring visual pattern consistencies and intrinsic temporal relationships across different frames. Furthermore, the semantic correspondence between natural language queries and person-centric actions in videos has not been fully explored. To address these problems, we propose a novel binary representation learning framework, named Semantics-aware Spatial-temporal Binaries ( [Formula: see text]Bin), which simultaneously considers spatial-temporal context and semantic relationships for cross-modal video retrieval. By exploiting the semantic relationships between two modalities, [Formula: see text]Bin can efficiently and effectively generate binary codes for both videos and texts. In addition, we adopt an iterative optimization scheme to learn deep encoding functions with attribute-guided stochastic training. We evaluate our model on three video datasets and the experimental results demonstrate that [Formula: see text]Bin outperforms the state-of-the-art methods in terms of various cross-modal video retrieval tasks.
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http://dx.doi.org/10.1109/TIP.2020.3048680DOI Listing
February 2021

Structural Diversity in Oxadiazole-Containing Silver Complexes Dependent on the Anions.

Acta Chim Slov 2020 Sep;67(3):822-829

Two coordination polymers, namely [Ag2(L)(SO3CF3)(H2O)](SO3CF3)•CH2Cl2 (1) and [Ag5(L)4(H2O)2](SbF6)5•5THF (2), were obtained by reacting oxadiazole-containing tri-armed ligand 1,3,5-tri(2-methylthio-1,3,4-oxadiazole-5yl) ben-zene (L) and silver salts in CH2Cl2/THF medium. The two complexes crystallized in the tetragonal space group I41/a and orthorhombic space group Fdd2, respectively. The Single-crystal X-ray diffraction revealed that the two complexes ex-hibit strikingly different 3D polymeric structures, which can be ascribed to the different counter anions. L in compound 1 acted as a hexa-dentate ligand, binding to two types of Ag+ atoms to form a 3D polymeric structure. L in compound 2acted as a hexa- and penta-dentate ligand, binding to three types of Ag+ atoms to form the 3D polymeric structure. The antibacterial activity of the complexes was also investigated.
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September 2020

Robot-assisted laparoscopic resection of a pelvic solitary fibrous tumor.

J Int Med Res 2021 Feb;49(2):300060520981479

Department of Urology, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China.

Solitary fibrous tumor (SFT) is a rare soft tissue neoplasm of mesenchymal origin. SFT is most commonly located in the thoracic cavity (in approximately 80% of cases), but can also develop rarely in the pelvis. A 47-year-old man presented to our hospital with a pelvic tumor that was discovered during a health checkup. We performed transperitoneal robotic resection of the pelvic tumor. Intraoperative blood loss and the console time were 100 mL and 2 hours 42 minutes, respectively, and no intraoperative or postoperative complications were recorded. Histologic analysis revealed a pelvic SFT with negative surgical margins. The patient was followed-up for 13 months with no evidence of tumor recurrence. To our knowledge, this is the first report of robot-assisted laparoscopic resection of a pelvic SFT.
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http://dx.doi.org/10.1177/0300060520981479DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7871082PMC
February 2021

Huge Free-Floating Thrombus in the Internal Carotid Artery Under Duplex Ultrasound Surveillance: A Case Report.

Neurologist 2020 Dec 30;26(1):22-23. Epub 2020 Dec 30.

Ultrasonography, Tianjin Huanhu Hospital, Tianjin, China.

Introduction: Carotid free-floating thrombus (FFT) is an unusual finding in acute ischemic stroke. Atherosclerosis is the most common etiology of FFT formation.

Case Report: Here we report a 42-year-old male patient admitted to our department with left temporal and parietal lobe ischemic stroke with normal magnetic resonance angiography. A huge FFT in the left internal carotid artery were found by duplex ultrasound. Acute thrombosis based on atherosclerotic plaque were considered as the reason of this embolization. The thrombus shrunk significantly under anticoagulation and antiplatelet treatment.

Conclusions: Evaluation of the intracranial vessel in the emergency is not enough and early carotid duplex ultrasound can help find of the FFT in time, which help to choose the early intervene by neurosurgeon. Early antithrombotic treatment can be a safe treatment option for reducing huge thrombus based on the nature of thrombus formation. Computed tomography angiography and high-resolution magnetic resonance imaging to certify the character of the plaque are recommended for plaque evaluation.
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http://dx.doi.org/10.1097/NRL.0000000000000296DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785710PMC
December 2020

Effect of liraglutide on endoplasmic reticulum stress in the renal tissue of type 2 diabetic rats.

World J Diabetes 2020 Dec;11(12):611-621

Department of Endocrinology, Shanxi Provincial People's Hospital, Taiyuan 030012, Shanxi Province, China.

Background: Liraglutide is a glucagon-like peptide 1 receptor agonist analog that has been found to have a therapeutic effect in diabetes. In addition to its ability to treat diabetes, liraglutide has beneficial effects on the cardiovascular system and kidney as well as other beneficial effects, but its specific mechanism is not clear. In this study, a rat model of type 2 diabetes was established by administration of a high-sugar, high-fat diet combined with low-dose streptozotocin (STZ) to observe the effect of liraglutide on the kidneys of type 2 diabetes rats and the possible underlying mechanisms.

Aim: To explore whether liraglutide has a protective effect on type 2 diabetic rat kidneys and the underlying mechanisms.

Methods: Eight-week-old male Sprague-Dawley rats were randomly divided into a control group, model group, low-dose liraglutide group, and high-dose liraglutide group. Control rats were fed a standard diet, while model group and intervention group rats were fed high-sugar, high-fat feed for 1 mo and then intraperitoneally injected with 40 mg/kg STZ to induce type 2 diabetes. The low-dose and high-dose intervention groups received 100 µg/kg and 200 µg/kg liraglutide, respectively, once daily by subcutaneous injection. The control and model groups were given an equivalent volume of physiological saline for 8 wk. Pathological changes in renal tissues were observed by hematoxylin and eosin staining and periodic acid-Schiff staining, and GRP78 and caspase-12 expression was detected by Western blot and reverse transcription-polymerase chain reaction (RT-PCR).

Results: Western blot analysis showed that GRP78 and caspase-12 protein expression in kidney tissue was significantly higher in model rats than in normal rats and lower in the liraglutide-treated groups than in the model group with a more significant decrease being observed in the high-dose group than in the low-dose group. RT-PCR showed that the mRNA expression of and was higher in model rats than in control rats and lower in the liraglutide-treated groups than in the model group, with the high-dose group exhibiting a more significant decrease than the low-dose group.

Conclusion: Liraglutide may delay the progression of diabetic nephropathy by reducing endoplasmic reticulum stress and protect the kidneys in a dose-dependent manner.
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http://dx.doi.org/10.4239/wjd.v11.i12.611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7754169PMC
December 2020

A clinical evaluation of noninvasive and contactless radiofrequency technique in the treatment of abdominal fat.

J Cosmet Dermatol 2020 Dec 23. Epub 2020 Dec 23.

The First Hospital of China Medical University, Shenyang, China.

Background: There is an increasing demand for fat reduction and body contouring procedures. Noninvasive radiofrequency devices have been used to tighten skin and treat cellulite, but there are few studies confirming their efficacy for abdominal fat reduction.

Objective: This study explored the effects of four noninvasive radiofrequency (RF) treatments on abdominal fat in Asian subjects, evaluating body weight, body mass index (BMI), and waist circumference.

Methods: In this study, 16 patients with abdominal obesity were treated four times with a noninvasive and contactless selective RF device (VANQUISH ME™, BTL Aesthetics). Treatments were 7 days apart and lasted 45 min each. The BMI and circumference of the upper, middle, and lower abdomen were measured at baseline and after each treatment.

Results: There were statistically significant reductions in BMI and abdominal circumference in all 16 patients (P < .05). Most patients only experienced a slight abdominal heat sensation and minimal body sweating during the treatment, and no adverse reactions were observed after the treatment.

Conclusion: The noninvasive and contactless selective RF technique was effective and safe in reducing fat, BMI, and abdominal circumference.
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http://dx.doi.org/10.1111/jocd.13892DOI Listing
December 2020

An accurate full-dimensional potential energy surface for the reaction OH + SO → H + SO.

Authors:
Jie Qin Jun Li

Phys Chem Chem Phys 2021 Jan;23(1):487-497

School of Chemistry and Chemical Engineering & Chongqing Key Laboratory of Theoretical and Computational Chemistry, Chongqing University, Chongqing 401331, China.

In this work, we report a full-dimensional accurate potential energy surface (PES-2020) for the reaction OH + SO → H + SO2, a prototype with deep complexes HOSO and HSO2. About 44 700 points are calculated at the level of UCCSD(T)-F12a/aug-cc-pVTZ and fitted by the permutation invariant polynomial-neural network (PIP-NN) approach. Particular attention is paid to the treatment of the electronic structure calculation so that the UCCSD(T)-F12a/aug-cc-pVTZ method can efficiently provide a reliable description for the ground electronic state of the title reaction. Comprehensive analyses and comparison show that the only available DMBE-PES is significantly different from the new PES-2020. Dynamics simulations on this new PES-2020 show that the reactivity decreases with the increase in collision energy. The isotropic product angular distributions remain within the studied collision energy range, 1-20 kcal mol-1, complying with the deep intermediates involved during the reaction process. The product energy partitioning is also analyzed. This accurate full-dimensional PES-2020 paves the way to fully understand the dynamics of the title reaction.
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http://dx.doi.org/10.1039/d0cp05206jDOI Listing
January 2021

Molecular Mechanisms of Glial Cells Related Signaling Pathways Involved in the Neuroinflammatory Response of Depression.

Mediators Inflamm 2020 10;2020:3497920. Epub 2020 Oct 10.

Department of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, Western University, London, Ontario, Canada.

Dysfunction of the glial cells, such as astrocytes and microglia, is one of the pathological features in many psychiatric disorders, including depression, which emphasizes that glial cells driving neuroinflammation is not only an important pathological change in depression but also a potential therapeutic target. In this review, we summarized a recent update about several signaling pathways in which glial cells may play their roles in depression through neuroinflammatory reactions. We focused on the basic knowledge of these signaling pathways by elaborating each of them. This review may provide an updated image about the recent advances on these signaling pathways that are essential parts of neuroinflammation involved in depression.
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http://dx.doi.org/10.1155/2020/3497920DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569467PMC
October 2020

Learning Multi-Attention Context Graph for Group-Based Re-Identification.

IEEE Trans Pattern Anal Mach Intell 2020 Oct 20;PP. Epub 2020 Oct 20.

Learning to re-identify a group of people across camera systems has important applications in video surveillance. However, most existing methods focus on person re-identification (re-id), ignoring the fact that people often walk in groups. In this work, we consider employing context information for group re-id. On the one hand, group re-id is more challenging than single person re-id, since it requires both a robust modeling of individual person and full awareness of global group structures. On the other hand, person re-id can be greatly enhanced by incorporating visual context, a task which we formulate as group-aware person re-id. In this paper, we propose a novel unified framework to simultaneously address the above tasks, i.e., group re-id and group-aware person re-id. Specifically, we construct a context graph to exploit dependencies among different people. A multi-level attention mechanism is developed to formulate both intra- and inter-group context, with an additional self-attention module for robust graph-level representations. Meanwhile, to facilitate the deployment of deep learning models on these tasks, we build a new group re-id dataset containing 3.8K images with 1.5K annotated groups. Extensive experiments on the novel dataset as well as three existing datasets clearly demonstrate the effectiveness of the proposed framework.
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http://dx.doi.org/10.1109/TPAMI.2020.3032542DOI Listing
October 2020

The Triplet Hydroxyl Radical Complex of Phosphorus Monoxide.

Angew Chem Int Ed Engl 2020 Dec 19;59(49):21949-21953. Epub 2020 Oct 19.

College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, China.

Phosphorus monoxide ( PO) is a key intermediate in phosphorus chemistry, and its association with the hydroxyl radical ( OH) to yield metaphosphorous acid (cis-HOPO) contributes to the chemiluminescence in the combustion of phosphines. When photolyzing cis-HOPO in an Ar-matrix at 2.8 K, the simplest dioxophosphorane HPO and an elusive hydroxyl radical complex (HRC) of PO form. This prototypical radical-radical complex reforms into cis-HOPO at above 12.0 K by overcoming a barrier of 0.28±0.02 kcal mol . The vibrational spectra of this HRC and its D- and O-isotopologues suggest a structure of OH⋅⋅⋅OP , for which a triplet spin multiplicity with a binding energy of -3.20 kcal mol has been computed at the UCCSD(T)-F12a/aug-cc-pVTZ level.
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http://dx.doi.org/10.1002/anie.202011512DOI Listing
December 2020

Aplasia cutis congenita with dystrophic epidermolysis bullosa: Bart syndrome.

Indian J Dermatol Venereol Leprol 2020 Sep 22. Epub 2020 Sep 22.

Department of Dermatology, No.1 Hospital of China Medical University, Shenyang, China.

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http://dx.doi.org/10.4103/ijdvl.IJDVL_1094_19DOI Listing
September 2020
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