Publications by authors named "Jie Pan"

693 Publications

Genomic insights into versatile lifestyle of three new bacterial candidate phyla.

Sci China Life Sci 2022 Jan 13. Epub 2022 Jan 13.

Archaeal Biology Center, Institute for Advanced Study, Shenzhen University, Shenzhen, 518060, China.

Metagenomic explorations of the Earth's biosphere enable the discovery of previously unknown bacterial lineages of phylogenetic and ecological significance. Here, we retrieved 11 metagenomic-assembled genomes (MAGs) affiliated to three new monophyletic bacterial lineages from the seawater of the Yap Trench. Phylogenomic analysis revealed that each lineage is a new bacterial candidate phylum, subsequently named Candidatus Qinglongiota, Candidatus Heilongiota, and Candidatus Canglongiota. Metabolic reconstruction of genomes from the three phyla suggested that they adopt a versatile lifestyle, with the potential to utilize various types of sugars, proteins, and/or short-chain fatty acids through anaerobic pathways. This was further confirmed by a global distribution map of the three phyla, indicating a preference for oxygen-limited or particle-attached niches, such as anoxic sedimentary environments. Of note, Candidatus Canglongiota genomes harbor genes for the complete Wood- Ljungdahl pathway and sulfate reduction that are similar to those identified in some sulfate-reducing bacteria. Evolutionary analysis indicated that gene gain and loss events, and horizontal gene transfer (HGT) play important roles in shaping the genomic and metabolic features of the three new phyla. This study presents the genomic insight into the ecology, metabolism, and evolution of three new phyla, which broadens the phylum-level diversity within the domain Bacteria.
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http://dx.doi.org/10.1007/s11427-021-2037-xDOI Listing
January 2022

Enhanced Spontaneous Antibacterial Activity of δ-MnO by Alkali Metals Doping.

Front Bioeng Biotechnol 2021 4;9:788574. Epub 2022 Jan 4.

College of Science, Donghua University, Shanghai, China.

Recently, the widespread use of antibiotics is becoming a serious worldwide public health challenge, which causes antimicrobial resistance and the occurrence of superbugs. In this context, MnO has been proposed as an alternative approach to achieve target antibacterial properties on mutans (S. mutans). This requires a further understanding on how to control and optimize antibacterial properties in these systems. We address this challenge by synthesizing δ-MnO nanoflowers doped by magnesium (Mg), sodium (Na), and potassium (K) ions, thus displaying different bandgaps, to evaluate the effect of doping on the bacterial viability of S. mutans. All these samples demonstrated antibacterial activity from the spontaneous generation of reactive oxygen species (ROS) without external illumination, where doped MnO can provide free electrons to induce the production of ROS, resulting in the antibacterial activity. Furthermore, it was observed that δ-MnO with narrower bandgap displayed a superior ability to inhibit bacteria. The enhancement is mainly attributed to the higher doping levels, which provided more free electrons to generate ROS for antibacterial effects. Moreover, we found that δ-MnO was attractive for applications, because it could nearly be degraded into Mn ions completely following the gradual addition of vitamin C. We believe that our results may provide meaningful insights for the design of inorganic antibacterial nanomaterials.
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http://dx.doi.org/10.3389/fbioe.2021.788574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764136PMC
January 2022

Genome-resolved evidence for functionally redundant communities and novel nitrogen fixers in the deyin-1 hydrothermal field, Mid-Atlantic Ridge.

Microbiome 2022 Jan 19;10(1). Epub 2022 Jan 19.

Archaeal Biology Center, Institute for Advanced Study, Shenzhen University, Shenzhen, Guangdong, People's Republic of China.

Background: Deep-sea hydrothermal vents represent unique ecosystems that redefine our understanding of the limits of life. They are widely distributed in deep oceans and typically form along mid-ocean ridges. To date, the hydrothermal systems in the Mid-Atlantic Ridge south of 14°S remain barely explored, limiting our understanding of the microbial community in this distinct ecosystem. The Deyin-1 is a newly discovered hydrothermal field in this area. By applying the metagenomic analysis, we aim at gaining much knowledge of the biodiversity and functional capability of microbial community inhabiting this field.

Results: In the current study, 219 metagenomic assembled genomes (MAGs) were reconstructed, unveiling a diverse and variable community dominated by Bacteroidetes, Nitrospirae, Alpha-, Delta-, and Gammaproteobacteria in the active and inactive chimney samples as well as hydrothermal oxide samples. Most of these major taxa were potentially capable of using reduced sulfur and hydrogen as primary energy sources. Many members within the major taxa exhibited potentials of metabolic plasticity by possessing multiple energy metabolic pathways. Among these samples, different bacteria were found to be the major players of the same metabolic pathways, further supporting the variable and functionally redundant community in situ. In addition, a high proportion of MAGs harbored the genes of carbon fixation and extracellular carbohydrate-active enzymes, suggesting that both heterotrophic and autotrophic strategies could be essential for their survival. Notably, for the first time, the genus Candidatus Magnetobacterium was shown to potentially fix nitrogen, indicating its important role in the nitrogen cycle of inactive chimneys. Moreover, the metabolic plasticity of microbes, diverse and variable community composition, and functional redundancy of microbial communities may represent the adaptation strategies to the geochemically complex and fluctuating environmental conditions in deep-sea hydrothermal fields.

Conclusions: This represents the first assembled-genome-based investigation into the microbial community and metabolism of a hydrothermal field in the Mid-Atlantic Ridge south of 14°S. The findings revealed that a high proportion of microbes could benefit from simultaneous use of heterotrophic and autotrophic strategies in situ. It also presented novel members of potential diazotrophs and highlighted the metabolic plasticity and functional redundancy across deep-sea hydrothermal systems. Video abstract.
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http://dx.doi.org/10.1186/s40168-021-01202-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8767757PMC
January 2022

Parenchymal Mass Loss During Partial Nephrectomy: Role of Devascularized Parenchymal Mass and Excised Parenchymal Mass and Impact on Functional Preservation.

Clin Genitourin Cancer 2021 Dec 16. Epub 2021 Dec 16.

Department of Urology, Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Clinical Research Center for Urinary Diseases, Guangzhou, China. Electronic address:

This study included 93 patients with renal masses who underwent standard partial nephrectomy or tumor enucleation. After surgery, parenchymal mass loss caused by devascularization resulted in more damage to renal function than excised parenchymal mass loss. Surgeons should seek better techniques to decrease devascularization during reconstruction.

Introduction: To evaluate the importance of devascularized parenchymal mass(DPM) and excised parenchymal mass(EPM) in functional preservation after standard partial nephrectomy(SPN).

Patients And Methods: Forty-one patients who underwent pure tumor enucleation(TE) and 52 patients who underwent SPN with necessary data were included. As no EPM was lost in TE, the TE samples were used to estimate the degree of volume shrinkage that occurred when the measurements were performed in vivo with blood flow versus ex vivo without, and the shrinkage ratio was calculated as specimen volume divided by tumor volume in vivo. In SPN, the specimen volume comprised tumor volume plus EPM. The EPM was calculated as specimen volume divided by shrinkage ratio minus tumor volume in vivo. The DPM was defined as total ipsilateral parenchymal mass loss minus EPM. T tests, χ test, and Mann-Whitney U tests were employed to compare clinical characteristics. Multivariate analysis was used to identify variables that correlated with glomerular filtration rate(GFR) preservation.

Results: The mean sizes of devascularized and excised parenchymal masses were 13.6 cm and 5.2 cm (P = .01), which accounted for 7.8% and 3.4% of preoperative ipsilateral parenchymal mass (P = .03) in SPN, respectively. The shrinkage ratio was 0.71 and correlation coefficient was 0.965. After stepwise regression, DPM, and preoperative GFR were significantly associated with global GFR preservation.

Conclusion: The DPM comprises most of parenchymal mass loss after SPN and plays a more important role than EPM on functional outcomes. Surgeons should pay more attention to reducing devascularization during partial nephrectomy.
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http://dx.doi.org/10.1016/j.clgc.2021.12.007DOI Listing
December 2021

Predictive value of nomogram based on Kyoto classification of gastritis to diagnosis of gastric cancer.

Scand J Gastroenterol 2022 Jan 7:1-7. Epub 2022 Jan 7.

Department of Gastroenterology, Wenzhou Central Hospital, Wenzhou, China.

Background And Aim: It is important to predict the risk of gastric cancer (GC) for endoscopists because early detection of GC determines the selection of the best treatment strategy and the prognosis of patients. The study aimed to evaluate the utility of a predictive nomogram based on the Kyoto classification of gastritis for GC.

Methods: It was a retrospective study that included 2639 patients who received esophagogastroduodenoscopy and serum pepsinogen (PG) assay from January 2019 to November 2019 at the Endoscopy Center of the Department of Gastroenterology, Wenzhou Central Hospital. Routine biopsy was conducted to determine the benign and malignant lesions pathologically. All cases were randomly divided into the training set (70%) and the validation set (30%) by using the bootstrap method. A nomogram was formulated according to multivariate analysis of the training set. The predictive accuracy and discriminative ability of the nomogram were assessed by concordance index (C-index), area under the curve (AUC) of receiver operating characteristic curve (ROC) as well as calibration curve and were validated by the validation set.

Results: Among all patients enrolled, 102 of 2636 cases showed LGIN, HGIN and gastric cancer pathology results, whereas the rest cases showed benign pathological results. Multivariate analysis indicated that age, sex, PG I/II ratio and Kyoto classification scores were independent predictive variables for GC. The C-index of the nomogram of the training set was 0.79 (95% CI: 0.74 to 0.84) and the AUC of ROC is 0.79. The calibration curve of the nomogram demonstrated an optimal agreement between predicted probability and observed probability of the risk of GC. The C-index was 0.86 (95% CI: 0.79 to 0.94) with a calibration curve of better concurrence in the validation set.

Conclusion: The nomogram formulated was proven to be of high predictive value for GC.
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http://dx.doi.org/10.1080/00365521.2021.2023626DOI Listing
January 2022

Relationship between the time point of left atrial size change and the outcomes of radiofrequency catheter ablation.

J Interv Card Electrophysiol 2022 Jan 7. Epub 2022 Jan 7.

Department of Cardiology, The First Affiliated Hospital of Shaoxing University, 312000, Shaoxing, Zhejiang, People's Republic of China.

Purpose: The change in the left atrial anteroposterior diameter (LAD) after radiofrequency catheter ablation (RFCA) for atrial fibrillation (AF) has become an independent factor in predicting the postoperative curative effect; however, whether the specific time point of this change is related to the postoperative curative effect is unclear. The purpose of this study was to explore the correlation between the specific time point of LAD change and the recurrence of AF 1 year after RFCA.

Methods: Patients with AF who underwent RFCA in our hospital from July 2016 to May 2020 were enrolled in the study. The patients were divided into four groups according to the time point when the LAD decreased by 10% after RFCA: group A, first month after RFCA; group B, second month after RFCA; group C, third month after RFCA; group D, unchanged or changed > 3 months after RFCA.

Results: In the multivariable Cox analyses, the duration of AF, LAD, and persistent AF were independent risk factors for the recurrence of AF. The recurrence rate of AF 1 year after RFCA were significantly lower in groups A (odds ratio [OR], 0.160; 95% confidence interval [CI]: 0.073-0.352; P < 0.001) and C (OR, 0.388; 95% CI: 0.156-0.963; P = 0.041) than in the control group.

Conclusions: Reduction of LAD within 3 months after operation predicts the success rate of RFCA, with reduction within 1 month after operation having a higher success rate. This indicates the necessity of treatment in patients with AF who do not experience an early reduction in the LAD after RFCA.
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http://dx.doi.org/10.1007/s10840-021-01097-6DOI Listing
January 2022

Oral administration of a whole glucan particle (WGP)-based therapeutic cancer vaccine targeting macrophages inhibits tumor growth.

Cancer Immunol Immunother 2022 Jan 4. Epub 2022 Jan 4.

State Key Laboratory of Genetic Engineering, Department of Pharmaceutical Science, School of Life Science, Fudan University, Shanghai, 200433, China.

Although therapeutic cancer vaccines have been gaining substantial ground, the development of cancer vaccines is impeded because of the undegradability of delivery systems, ineffective delivery of tumor antigens and weak immunogenicity of adjuvants. Here, we made use of a whole glucan particle (WGP) to encapsulate ovalbumin (OVA), thereby formulating a novel cancer vaccine. Results from in vitro experiments showed that WGP-OVA not only induced the activation of bone marrow-derived macrophages (BMDMs) including driving M0 BMDM polarization to the M1 phenotype, upregulating the costimulatory molecules and inducing the generation of cytokines, but also facilitated antigen presentation. After oral administration of the WGP-OVA formulation to mice with OVA-expressing tumors, these particles can increase tumor-infiltrating OVA-specific CD8 CTLs and repolarize tumor-associated macrophages (TAMs) toward M1-like phenotype, which led to delayed tumor progression. These findings revealed that WGP could serve as both an antigen delivery system and an adjuvant system for promising cancer vaccines.
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http://dx.doi.org/10.1007/s00262-021-03136-7DOI Listing
January 2022

Evaluation of Urea-Based Inhibitors of the Dopamine Transporter Using the Experimental Autoimmune Encephalomyelitis Model of Multiple Sclerosis.

ACS Chem Neurosci 2022 01 3;13(2):217-228. Epub 2022 Jan 3.

Department of Pharmaceutical Sciences, Jerry H. Hodge School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, Texas 79106, United States.

The dopaminergic system is involved in the regulation of immune responses in various homeostatic and disease conditions. For conditions such as Parkinson's disease and multiple sclerosis (MS), pharmacological modulation of dopamine (DA) system activity is thought to have therapeutic relevance, providing the basis for using dopaminergic agents as a treatment of relevant states. In particular, it was proposed that restoration of DA levels may inhibit neuroinflammation. We have recently reported a new class of dopamine transporter (DAT) inhibitors with high selectivity to the DAT over other G-protein coupled receptors tested. Here, we continue their evaluation as monoamine transporter inhibitors. Furthermore, we show that the urea-like DAT inhibitor (compound ) has statistically significant anti-inflammatory effects and attenuates motor deficits and pain behaviors in the experimental autoimmune encephalomyelitis model mimicking clinical signs of MS. To the best of our knowledge, this is the first study reporting the beneficial effects of DAT inhibitor-based treatment in animals with induced autoimmune encephalomyelitis, and the observed results provide additional support to the model of DA-related neuroinflammation.
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http://dx.doi.org/10.1021/acschemneuro.1c00647DOI Listing
January 2022

Acoustical intensity probe based on a polyvinylidene fluoride bimorph.

J Acoust Soc Am 2021 Dec;150(6):4083

Department of Mechanical Engineering, University of Western Australia, Crawley, WA 6009, Australia.

This study investigates complex acoustical intensity using a polyvinylidene fluoride (PVDF) bimorph. Analytical models of the open-circuit voltage outputs of an infinite-strip-shaped PVDF bimorph cantilever in an underwater sound field are developed. Results show that the sound pressure generates the sum of the outputs, while the particle velocity normal to the PVDF surface generates the difference. The sensitivities of the pressure- and velocity-generated voltage responses with respect to an incident plane sound field demonstrate uniform directivity in a low-frequency range, which is suitable for acoustical intensity determination. The higher velocity sensitivity confirms the advantage of using a PVDF bimorph as a velocity sensor, owing to its light weight and flexibility. An algorithm for determining the complex acoustical intensity normal to the surface is proposed by utilizing those voltage responses and the probe gain calibrated with a given angle of incident sound. This algorithm allows accurate determination of sound intensity of a plane wave field, where the reactive part of intensity is absent. However, a small error may exist when the reactive intensity is large and active intensity is small. This small discrepancy arises from the inherent variation in the phase directivity of the gains, which decrease with frequency.
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http://dx.doi.org/10.1121/10.0007483DOI Listing
December 2021

Aidi injection altered the activity of CYP2D4, CYP1A2, CYP2C19, CYP3A2, CYP2E1 and CYP2C11 in normal and diethylnitrosamine-induced hepatocellular carcinoma in rats.

J Ethnopharmacol 2022 Mar 21;286:114930. Epub 2021 Dec 21.

The Affiliated Hospital of Guizhou Medical University, 28(#) Guiyi Road, Guiyang, 550004, Guizhou, China; School of Pharmacy, Guizhou Medical University, No.9, Beijing Road, Yunyan District, Guiyang, 550004, China. Electronic address:

Ethnopharmacological Relevance: Aidi injection (ADI), a traditional chinese medicine preparation, is widely used in combination with chemotherapy for the treatment of various malignant tumors, such as hepatocellular carcinoma (HCC). Studies have shown that changes in cytochrome P450 (CYP450) activity in disease states would affect the metabolism of drugs in vivo, especially liver diseases. However, the changes of Aidi injection on the activities of CYP2D4, CYP1A2, CYP2C19, CYP3A2, CYP2E1 and CYP2C11 in normal and HCC states are still unknown.

Aim Of The Study: The cocktail probe drugs method was used to investigate the effects of ADI on the activity of CYP2D4, CYP1A2, CYP2C19, CYP3A2, CYP2E1 and CYP2C11 in normal and HCC rats.

Materials And Methods: The HCC rats was induced by diethylnitrosamine (DEN). Then, both normal and HCC rats were randomly divided into 2 groups (n = 6). They were given saline or ADI (10 mL/kg/d, i.p) for 2 weeks, respectively. On the fifteenth day, cocktail probe mixing solution, including metoprolol (10 mg/kg), caffeine (1.0 mg/kg), omeprazole (2.0 mg/kg), midazolam (2.0 mg/kg), chlorzoxazone (4.0 mg/kg) and tolbutamide (0.5 mg/kg), was injected into tail vein of all rats in each group. The blood sample was obtained at specified time. After the protein is precipitated, six probe drugs are analyzed by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS).

Results: Compared with control group, the activity of CYP3A2 and CYP2E1 was significantly lower in the ADI group. Compared with the model group, the activities of CYP1A2, CYP3A2, CYP2E1, and CYP2C11 enzymes in the ADI model group were significantly reduced. Additionally, the activity of CYP2D4, CYP1A2, CYP2C19, CYP3A2, CYP2E1 and CYP2C11 enzymes in model group was significantly lower than control group.

Conclusions: ADI can inhibit a lot of CYP450 enzyme, so it may reduce the dosage of chemotherapeutic drugs to reach the required plasma concentration of chemotherapeutic drugs, which is of great significance for the combination of anti-tumor chemotherapeutic drugs and is worthy of further in-depth study and clinical attention.
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http://dx.doi.org/10.1016/j.jep.2021.114930DOI Listing
March 2022

Record-High Superconductivity in Transition Metal Dichalcogenides Emerged in Compressed 2H-TaS.

Adv Mater 2021 Dec 22:e2103168. Epub 2021 Dec 22.

State Key Laboratory of Superhard Materials, Jilin University, Changchun, 130012, China.

Pressure has always been an effective method for uncovering novel phenomena and properties in condensed matter physics. Here, we carried out an electrical transport study on 2H-TaS up to ∼208 GPa, and found an unexpected superconducting state (SC-II) emerging around 86.1 GPa with an initial critical temperature (T ) of 9.6 K. As pressure increases, the T enhances rapidly and reaches the maximum of 16.4 K at 157.4 GPa, which sets a new record for transition metal dichalcogenides (TMDs). The original superconducting state (SC-I) was found to be re-enhanced above 100 GPa after the recession around 10 GPa, and coexists with SC-II to the highest pressure applied in this work. In situ high-pressure X-ray diffraction and Hall effect measurements reveal that the occurrence of SC-II is accompanied by a structural modification and a concurrent enhancement of hole carrier density. The new high-T superconducting state that emerged in 2H-TaS can be attributed to the electronic states change near the Fermi surface owing to the pressure-induced interlayer modulation. It is the first time finding this remarkable superconducting state in TMDs, which not only brings a new broad of perspective on layered materials but also expands the field of pressure-modified superconductivity. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202103168DOI Listing
December 2021

Protective effects on ovalbumin-induced mouse asthma models and qualitative and quantitative analysis of multiple compounds in Gerberae Piloselloidis Herba.

J Sep Sci 2021 Dec 22. Epub 2021 Dec 22.

State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University, Guiyang, P. R. China.

Gerberae Piloselloidis Herba is widely used to treat cough and asthma in China. However, its effects on allergic asthma as related to its chemical compositions have not been fully elucidated, and there is a scarcity of methods to determine multi-component contents for quality control. In this study, protective effects of Gerberae Piloselloidis Herba on ovalbumin-induced asthma models were investigated, while qualitative and quantitative analyses of multiple constituents in Gerberae Piloselloidis Herba were conducted by using an ultrahigh-performance liquid chromatography-Q Exactive hybrid quadrupole-orbitrap high-resolution accurate mass spectrometry and an ultrahigh-performance liquid chromatography-photodiode array detection. The results showed that Gerberae Piloselloidis Herba could significantly mitigate asthma symptoms, reduce eosinophils counts in the bronchoalveolar lavage fluid, as well as decrease IgE, IL-5, and IL-13 concentration, and inflammatory cellular infiltration in lung tissues. A total of 51 compounds were tentatively identified, in which the content of 10 representative compounds was determined in 24 batches of Gerberae Piloselloidis Herba by using an ultrahigh-performance liquid chromatography method with good linearity, precision, repeatability, accuracy, and stability. This research presents a comprehensive strategy combining biological activity evaluation with chemical profiling, providing a useful and comprehensive reference for further application and quality control of Gerberae Piloselloidis Herba.
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http://dx.doi.org/10.1002/jssc.202100392DOI Listing
December 2021

The Bioinformatics-Based Analysis Identifies 7 Immune-Related Genes as Prognostic Biomarkers for Colon Cancer.

Front Oncol 2021 26;11:726701. Epub 2021 Nov 26.

Department of Medical Oncology, Fujian Medical University Union Hospital, Fuzhou, China.

Colon cancer poses a great threat to human health. Currently, there is no effective treatment for colon cancer due to its complex causative factors. Immunotherapy has now become a new method for tumor treatment. In this study, 487 DEGs were screened from The Cancer Genome Atlas (TCGA) database and ImmPort database, and GeneOntology (GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was performed. Hierarchical clustering of all samples revealed a significant correlation between colon cancer and immunity. The weighted gene co-expression network analysis (WGCNA) algorithm was used to identify key gene modules associated with immunity in colon cancer, here, module grey60 showed the highest correlation. A protein-protein interaction (PPI) network was constructed using the STRING database to screen hub genes, and subsequently, 7 immune-related genes the most closely associated with colon cancer were identified by differential expression in cancer and paracancer. Finally, a risk prediction model was developed using least absolute shrinkage and selection operator (LASSO) COX analysis, and the accuracy of the model was validated by GSE14333. This study determined that IRF4 and TNFRSF17 were immune-related genes in colon cancer, providing immune-related prognostic biomarkers for colon cancer.
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http://dx.doi.org/10.3389/fonc.2021.726701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8663025PMC
November 2021

Clinical features and prognostic factors of systemic sclerosis in Guangxi, China: Retrospective, single-center study of long-term survival in 470 patients.

Int J Rheum Dis 2021 Dec 10. Epub 2021 Dec 10.

The Department of Rheumatology, First Affiliated Hospital, Guangxi Medical University, Nanning, China.

Objective: Systemic sclerosis (SSc) is an autoimmune disease the prevalence of which varies among populations. We analyzed SSc patients from Guangxi to improve the clinical understanding of this disease.

Methods: Data of 470 SSc patients admitted to our institution from October 1,2012 to January 1,2019 were examined. The characteristics of these patients were analyzed using Kaplan-Meier survival analysis. Cox proportional-hazard regression was used to identify prognostic factors.

Results: The average age was 50.44 ± 12.31 years, 285 patients (60.6%) were women, 2.1% had pneumoconiosis, 58.2% had pulmonary interstitial disease (ILD), 18.7% had pulmonary hypertension (PH), and 3.6% had renal crisis. These patients had diffuse cutaneous systemic sclerosis (dcSSc, 70.2%) or limited cutaneous systemic sclerosis (29.7%), and PH and renal crisis were more common in the dcSSc group. Patients 50 years old or more had greater prevalences of ILD, PH, and musculoskeletal damage, greater positivity of laboratory biomarkers, and increased mortality (all P < .05). Seventy-four patients (15.7%) died. The non-survivors were older, had longer disease duration, had higher prevalences of ILD, restrictive ventilation dysfunction, PH, and renal crisis, and had higher levels of creatine kinase myocardial band (CK-MB), C-reactive protein, and immunoglobin A (all P < .05). Renal crisis, PH, and high CK-MB were independent risk factors for death.

Conclusions: Pneumoconiosis was more common in SSc patients than the general population from this region. Our patients had a 10-year cumulative survival rate of 74.9%, higher than reported for patients from the US. Renal crisis, PH, and high CK-MB level were independent risk factors for death.
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http://dx.doi.org/10.1111/1756-185X.14261DOI Listing
December 2021

Sensitivity of an infinite-strip-shaped polyvinylidene fluoride film in an underwater plane sound field.

J Acoust Soc Am 2021 Nov;150(5):3343

Department of Mechanical Engineering, University of Western Australia, Crawley, WA 6009, Australia.

The sensitivity of an infinite-strip-shaped polyvinylidene fluoride (PVDF) film in an underwater plane sound field is analyzed in this paper. The high-frequency sensitivity is characterized by the resonances of the symmetrical in-plane stress modes with traction-free boundary conditions at the ends of the film and by the anti-resonances due to the superposition of the stress components. The low-frequency sensitivity exhibits a simple hydrostatic response. The directional property of the in-plane stress component, generated by the incident sound across the thickness, has two contributions. The first is from the aperture and dipolar functions of the incident sound pressure over the PVDF surfaces and at the two ends of the film. The second is from an amplitude modulation by an angle-dependent gain. The directional property of the in-plane stress component by the sound pressure at the two ends of the film is only controlled by the dipolar function, and that of the stress component in the thickness direction is only determined by the aperture function. The illustration of the frequency and directional features of the PVDF film may advance understanding of the mechanisms involved in generating the voltage output of PVDF by an incident sound field.
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http://dx.doi.org/10.1121/10.0006778DOI Listing
November 2021

Modulating the structure and photochromic performance of hybrid metal chlorides with nonphotochromic 1,10-phenanthroline and its derivative.

Dalton Trans 2021 Dec 14;50(48):18089-18096. Epub 2021 Dec 14.

College of Chemistry and Chemical Engineering, Qingdao University, Shandong 266071, P. R. China.

Hybrid photochromic materias (HPMs), especially crystalline HPMs (CHPMs), have been widely investigated due to their feasibility in maintaining the advantages of each constituent and genearating captivating photomodulated functionality. Metal-organic complexes (MOCs), as promising candidates for fabricating CHPMs, have attracted the interest of researchers. The molecular predesign of ligands plays a crucial role in yielding MOC-based CHPMs with tunable photochromic functionality. Hitherto, a great majority of CHPMs are driven by photosensitive ligands. However, the complicated synthesis and high cost of photosensitive ligands obviously prevent the macro-synthesis and future application of these CHPMs. Thus, it is indispensable to explore novel branches of CHPMs. Herein, we report a series of photochromic solid materials bearing modulated photochromic properties by hybridizing metal chlorides with a nonphotosensitive coplanar dipyridine unit 1,10-phenanthroline (phen) and its derivative 5-chloro-1,10-phenanthroline (5-Cl-phen). The resulting hybrids, [ZnCl(phen)] (1), [CdCl(phen)] (2), [PbCl(phen)] (3), [ZnCl(HO)(5-Cl-phen)]Cl·2HO (4), [CdCl(5-Cl-phen)] (5) and [PbCl(5-Cl-phen)] (6), exhibit distinct structures from the isolated molecular complexes (1 and 4) to the hybrid chain (2, 3, 5 and 6) because of the distinct coordination mode of central metal ions and chloride ions. After photo-irradiation with a Xe-lamp, all complexes, as expected, exhibited apparent color change because of the photoinduced electron transfer (ET) between coordinated chloride ions (Cl) as electron donors (EDs) and the coordinated coplanar phen and 5-Cl-phen species as electron acceptors (EAs). More importantly, the photochromic performance of the title complexes could be modulated by phen and 5-Cl-phen. This study provides a general and facile way for modulating the structure and photochromic performance of hybrid metal chlorides with phen or phen-based derivatives under the synergy of crystalline engineering strategy and ET mechanism.
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http://dx.doi.org/10.1039/d1dt02899eDOI Listing
December 2021

Expert consensus on thermal ablation therapy of pulmonary subsolid nodules (2021 Edition).

J Cancer Res Ther 2021 Nov;17(5):1141-1156

Department of Thoracic Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China.

The Expert Consensus reviews current literatures and provides clinical practice guidelines for thermal ablation of pulmonary subsolid nodules or ground-glass nodule (GGN). The main contents include the following: (1) clinical evaluation of GGN; (2) procedures, indications, contraindications, outcomes evaluation, and related complications of thermal ablation for GGN; and (3) future development directions.
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http://dx.doi.org/10.4103/jcrt.jcrt_1485_21DOI Listing
November 2021

Effects of desensitizing dentifrices on dentin tubule occlusion and resistance to erosive challenges.

BMC Oral Health 2021 11 30;21(1):610. Epub 2021 Nov 30.

University of Rochester Eastman Institute for Oral Health, 625 Elmwood Ave, Rochester, NY, 14620, USA.

Background: Many studies have demonstrated efficacy of casein phosphopeptide (CPP) containing products for dentin tubule occlusion for treatment of dentin sensitivity, but their effectiveness under dynamic erosive challenges remains to be elucidated. The purpose of the present study was to investigate the effectiveness of a desensitizing dentifrice containing CPP in occluding dentin tubules and resisting erosive challenges in comparison to that containing polyvinyl methyl ether/maleic acid (PVM/MA) copolymers.

Methods: A total of 33 dentin discs were prepared from coronal sections of human third molars and divided into 3 groups: a toothpaste containing CPP; a toothpaste containing PVM/MA and submicron silica; and a regular toothpaste (Controls). A soft-bristle toothbrush was used to brush the dentin discs with the dentifrices for 45 strokes in 30 s at a force of approximately 200 g. The brushing cycle was repeated after immersion of the dentin discs in artificial saliva overnight. The dentin discs were then challenged in orange juice for 10 min in an incubator rocking at 120 rpm. Three fields were randomly selected on each dentin disk surface to assess dentin tubule occlusions after each brushing cycle and after orange juice challenge with a 3D laser scanning microscope. Specimen cross sections were examined with a scanning electron microscope equipped with energy dispersive spectroscopy (SEM/EDS).

Results: After the first and second cycles of brushing, dentin tubules were occluded on average by 56.3% and 85.7% in CPP group, 66.2% and 88.1% in PVM/MA group, and 0.0 and 13.0% in the controls, respectively. There were no statistically significant differences in dentin tubule occlusions between the CPP and PVM/MA groups after two cycles of brushing (p>0.05). After dynamic erosive challenges with orange juice, 20.3% of the dentin tubules in the CPP group, 79.1% in the PVM/MA group and none in the control remained occluded (P<0.05). SEM/EDS imaging showed that dentin tubules were blocked with plugs containing dentifrice substances in CPP and PVM/MA groups after treatments, but none in the controls.

Conclusions: Desensitizing dentifrices containing CPP or PVM/MA could effectively occlude dentin tubules after two cycles of brushing. PVM/MA in combination with submicron silicon dioxide exhibited stronger resistance to dynamic erosive challenges by acidic beverages. Inorganic fillers that can enter dentin tubules and resist erosive challenges may be key for desensitizing dentifrices.
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http://dx.doi.org/10.1186/s12903-021-01977-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8638163PMC
November 2021

Activatable Second Near-Infrared Fluorescent Probes: A New Accurate Diagnosis Strategy for Diseases.

Biosensors (Basel) 2021 Nov 2;11(11). Epub 2021 Nov 2.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Center for Molecular Imaging, Translational Medicine School of Public Health, Xiamen University, Xiamen 361102, China.

Recently, second near-infrared (NIR-II) fluorescent imaging has been widely applied in biomedical diagnosis, due to its high spatiotemporal resolution and deep tissue penetration. In contrast to the "always on" NIR-II fluorescent probes, the activatable NIR-II fluorescent probes have specific targeting to biological tissues, showing a higher imaging signal-to-background ratio and a lower detection limit. Therefore, it is of great significance to utilize disease-associated endogenous stimuli (such as pH values, enzyme existence, hypoxia condition and so on) to activate the NIR-II probes and achieve switchable fluorescent signals for specific deep bioimaging. This review introduces recent strategies and mechanisms for activatable NIR-II fluorescent probes and their applications in biosensing and bioimaging. Moreover, the potential challenges and perspectives of activatable NIR-II fluorescent probes are also discussed.
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http://dx.doi.org/10.3390/bios11110436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615551PMC
November 2021

Preparation and surface-enhanced Raman scattering properties of GO/Ag/TaO composite substrates.

Opt Express 2021 Oct;29(21):34552-34564

The composite substrate composed of precious metal, semiconductor and graphene has not only high sensitivity and uniform Raman signal but also stable chemical properties, which is one of the important topics in the field of surface-enhanced Raman scattering (SERS). In this paper, a sandwich SERS substrate based on tantalum oxide (TaO) is designed and fabricated. The substrate has high sensitivity, stable performance and high quantification capability. The composite substrate can achieve a high sensitivity Raman detection of crystal violet (CV) with a detection limit of 10 M and an enhancement factor of 1.5 × 10. This is the result of the synergistic effect of electromagnetic enhancement and chemical enhancement, in which the chemical enhancement is the cooperative charge transfer in the system composed of probe molecules, silver nanoparticles (AgNPs) and TaO, and the electromagnetic enhancement comes from the strong local surface plasmon resonance between the adjacent AgNPs. After exposing the composite substrate to the air for one month, the Raman signal did not weaken, indicating that the performance of the composite substrate is stable. In addition, there is an excellent linear relationship between the intensity of Raman characteristic peak and the concentration of probe molecules, which proves that the composite substrate has high quantification capability. In practical application, the composite SERS substrate can be used to detect harmful malachite green quickly and sensitively and has a broad application prospect in the field of food safety and chemical analysis.
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http://dx.doi.org/10.1364/OE.435662DOI Listing
October 2021

A Retrospective Study of Oral Emergency Services During COVID-19.

Int Dent J 2021 Sep 28. Epub 2021 Sep 28.

Department of Oral Emergency, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing, P.R. China. Electronic address:

Objectives: This study was performed to examine changes in the number of patient visits and types of oral services in an oral emergency department from the beginning to the control stage of the coronavirus disease 2019 (COVID-19) outbreak in Beijing.

Methods: The numbers of daily oral emergency visits from January 20 to March 24, 2020, at a dental university hospital in Beijing and daily newly confirmed COVID-19 cases in Beijing during the same period were collected and analysed. All oral emergency patient information (including sex, age, and oral diagnosis) was also collected and analysed. Patients with incomplete medical data were excluded.

Results: In total, 12,416 patients were included in this study. The number of daily emergency visits was negatively correlated with the number of newly confirmed local COVID-19 cases in Beijing (P < .001). The number of daily emergency visits during the COVID-19 stable period in Beijing was greater than that during the outbreak period (P < .001). Compared to those in the COVID-19 outbreak period, the percentages of females, children and adolescents, patients with acute toothache, and patients with nonurgent cases were higher in the stable period, and the numbers of patients with toothache, trauma, infection, and nonemergency conditions increased in the COVID-19 stable period (P < .001).

Conclusions: COVID-19 significantly influenced the number of patient visits and the percentages of patients with oral emergency situations in the oral emergency department. There were obvious differences in treatment seeking for oral emergencies between the COVID-19 periods in Beijing. There was an inverse relationship between daily oral emergency visits and daily confirmed COVID-19 cases in Beijing.
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http://dx.doi.org/10.1016/j.identj.2021.09.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8483898PMC
September 2021

The stress granule protein G3BP1 promotes pre-condensation of cGAS to allow rapid responses to DNA.

EMBO Rep 2022 Jan 15;23(1):e53166. Epub 2021 Nov 15.

State Key Laboratory of Proteomics, National Center of Biomedical Analysis, Beijing, China.

Cyclic GMP-AMP synthase (cGAS) functions as a key sensor for microbial invasion and cellular damage by detecting emerging cytosolic DNA. Here, we report that GTPase-activating protein-(SH3 domain)-binding protein 1 (G3BP1) primes cGAS for its prompt activation by engaging cGAS in a primary liquid-phase condensation state. Using high-resolution microscopy, we show that in resting cells, cGAS exhibits particle-like morphological characteristics, which are markedly weakened when G3BP1 is deleted. Upon DNA challenge, the pre-condensed cGAS undergoes liquid-liquid phase separation (LLPS) more efficiently. Importantly, G3BP1 deficiency or its inhibition dramatically diminishes DNA-induced LLPS and the subsequent activation of cGAS. Interestingly, RNA, previously reported to form condensates with cGAS, does not activate cGAS. Accordingly, we find that DNA - but not RNA - treatment leads to the dissociation of G3BP1 from cGAS. Taken together, our study shows that the primary condensation state of cGAS is critical for its rapid response to DNA.
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http://dx.doi.org/10.15252/embr.202153166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8728604PMC
January 2022

Upregulated Expression of Cancer-Derived Immunoglobulin G Is Associated With Progression in Glioma.

Front Oncol 2021 25;11:758856. Epub 2021 Oct 25.

Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Objective: Gliomas are the most aggressive intracranial tumors accounting for the vast majority of brain tumors with very poor prognosis and overall survival (OS). Cancer-derived immunoglobulin G (cancer-IgG) has been found to be widely expressed in several malignancies such as breast cancer, colorectal cancer, and lung cancer. Cancer-IgG could promote tumorigenesis and progression. However, its role in glioma has not been revealed yet.

Methods: We mined open databases including the Chinese Glioma Genome Atlas (CGGA), The Cancer Genome Atlas (TCGA), and the Gene Expression Omnibus (GEO) to study the role of , which encodes cancer-IgG in glioma. Examination of the differential expression of was carried out in the GEO and TCGA databases. Furthermore, its expression in different molecular subtypes was analyzed. Stratified analysis was performed with clinical features. Subsequently, immune infiltration analysis was conducted using single-sample gene set enrichment analysis (ssGSEA). GSEA was performed to reveal the mechanisms of . Lastly, immunohistochemistry was processed to validate our findings.

Results: In this study, we found that the expression of was higher in glioma and molecular subtypes with poor prognosis. The overall survival of patients with a high expression of was worse in the stratified analysis. Immune infiltration analysis indicated that the expression level of was positively correlated with the stromal score, ESTIMATE score, and immune score and negatively correlated with tumor purity. Results from the GSEA and DAVID demonstrated that may function in phagosome, antigen processing and presentation, extracellular matrix structural constituent, antigen binding, and collagen-containing extracellular matrix. Finally, immunohistochemistry assay validated our findings that patients with a high expression of cancer-IgG had poor OS and disease-free survival (DFS).

Conclusion: Cancer-IgG is a promising biomarker of diagnosis and treatment for patients with glioma.
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http://dx.doi.org/10.3389/fonc.2021.758856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8574069PMC
October 2021

Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction.

Mol Ther Nucleic Acids 2021 Dec 7;26:970-986. Epub 2021 Sep 7.

Greater Bay Biomedical Innocenter, Shenzhen Bay Laboratory, Shenzhen, Guangdong Province, China.

Blood-brain barrier (BBB) dysfunction is associated with an accumulation of neurotoxic molecules and increased infiltration of peripheral cells within the brain parenchyma. Accruing evidence suggests that microglia and astrocytes play a crucial role in the recovery of BBB integrity and the corralling of infiltrating cells into clusters after brain damage, but the mechanisms involved remain unclear. Intriguingly, the results of flow cytometry and immunofluorescence analyses have shown that BBB permeability to peripheral cells is substantially enhanced during normal aging at 12 months in mice. Thus, we used the SMART-seq2 method to perform RNA sequencing of microglia and astrocytes at five time points before and immediately after the BBB permeability change. Our comprehensive analyses revealed that microglia are characterized by marked alterations in the negative regulation of protein phosphorylation and phagocytic vesicles, whereas astrocytes show elevated enzyme or peptidase-inhibitor activity in the recovery of BBB function. Moreover, we identified a cassette of key genes that might ameliorate the insults of pathophysiological events in aging and neurodegenerative disease.
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http://dx.doi.org/10.1016/j.omtn.2021.08.030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8561003PMC
December 2021

[Mechanism of Liangfu Dropping Pills in treatment of gastrointestinal diseases based on plasma pharmacochemistry and network pharmacology].

Zhongguo Zhong Yao Za Zhi 2021 Oct;46(20):5393-5402

State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine (Ministry of Education), Guizhou Medical University Guiyang 550004, China.

To study the active chemical components and mechanism of Liangfu Dropping Pills in treatment of gastrointestinal diseases. The UHPLC-Q-TOF-MS method was employed to analyze the components of Liangfu Dropping Pills in plasma. The protein targets of the absorbed compounds were predicted in the TCMSP database and the SwissTargetPrediction database. The targets associated with gastrointestinal diseases were collected from OMIM, CTD, GeneCards, and DrugBank. The common target genes between components and diseases were screened out for the building of protein-protein interaction(PPI) network in the STRING database. Metascape was used to carry out gene ontology(GO) and Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment analysis. Cytoscape was employed to construct the PPI network diagram and absorbed component-target network diagram. The molecular docking between the components absorbed in blood and potential key targets was performed by AutoDock vina 4.2.6 to screen and verify the main active components and targets. Twelve chemcial components were identified in Liangfu Dropping Pills, in which four components were absorbed in blood, including galangin, rhamnocitrin, galangin 3-methyl ether, and α-cyperone. These components acted on 189 common targets which were mainly involved in the cell responses to nitrogen compounds, organic cyclic compounds, and hormones, and enriched in the PI3 K-Akt signaling pathway, Foxo signaling pathway, and IL-17 signaling pathway. Molecular docking results showed that the four components had strong affinity with core targets. The material basis of Liangfu Dropping Pills treating gastrointestinal diseases may be galangin, rhamnocitrin, galangin 3-methyl ether, and α-cyperone. This study provides a theoretical basis for further development and application of Liangfu Dripping Pills.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210705.201DOI Listing
October 2021

Dynamic SARS-CoV-2-specific B-cell and T-cell responses following immunization with an inactivated COVID-19 vaccine.

Clin Microbiol Infect 2021 Oct 26. Epub 2021 Oct 26.

Department of Laboratory Medicine, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, Jiangsu, China. Electronic address:

Objective: The dynamic adaptive immune responses elicited by the inactivated virus vaccine CoronaVac remain elusive.

Methods: In a prospective cohort of 100 healthcare professionals naïve for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) who received two doses of CoronaVac, we analysed SARS-CoV-2-specific humoral and cellular responses at four different timepoints, including before vaccination (T1), 2 weeks after the first dose (T2), 2 weeks after the booster dose (T3), and 8-10 weeks after the booster dose (T4). SARS-CoV-2-specific antibodies, serum neutralizing activities, peripheral B cells, CD4 and CD8 T cells and their memory subsets were simultaneously measured in this cohort.

Results: SARS-CoV-2 spike-specific IgG responses reached a peak (geometric mean titre (GMT) 54827, 30969-97065) after two doses and rapidly declined (GMT 502, 212-1190) at T4, whereas suboptimal IgA responses were detected (GMT 5, 2-9). Spike-specific circulating B cells (0.60%, 0.46-0.73% of total B cells) and memory B cells (1.18%, 0.92-1.44% of total memory B cells) were effectively induced at T3 and sustained over time (0.33%, 0.23-0.43%; 0.87%, 0.05-1.67%, respectively). SARS-CoV-2-specific circulating CD4 T cells (0.57%, 0.47-0.66%) and CD8 T cells (1.29%, 1.04-1.54%) were detected at T3. At T4, 0.78% (0.43-1.20%) of memory CD4+ T cells and 0.68% (0.29-1.30%) of memory CD8+ T cells were identified as SARS-CoV-2-specific, while 0.62% (0.51-0.75%) of CD4 T cells and 0.47% (0.38-0.58%) of CD8 T cells were SARS-CoV-2-specific terminally differentiated effector memory cells. Furthermore, age and interval between doses affected the magnitude of CoronaVac-induced immune responses. SARS-CoV-2 memory CD4 T cells were strongly associated with both receptor binding domain (RBD)-specific memory B cells (r 0.87, p <0.0001) and SARS-CoV-2-specific memory CD8 T cells (r 0.48, p <0.0001).

Conclusions: CoronaVac induced robust circulating and memory B cell and T cell responses. Our study offers new insight into the underlying immunobiology of inactivated virus vaccines in humans and may have implications for vaccine strategies in the future.
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http://dx.doi.org/10.1016/j.cmi.2021.10.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8547974PMC
October 2021

Dabigatran plasma concentration indicated the risk of patients with non-valvular atrial fibrillation.

Heart Vessels 2021 Oct 25. Epub 2021 Oct 25.

Department of Pharmacy, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, Jiangsu, China.

This study aimed to evaluate the variability of dabigatran plasma concentration and the association with clinical events in Chinese patients treated with dabigatran etexilate (DE) for non-valvular atrial fibrillation (NVAF). The steady-state concentration of dabigatran (the active metabolite of DE) was determined at trough and peak. The effect of dabigatran concentration variability and related factors on clinical outcomes were explored. Data from 86 patients receiving a fixed dose of 110 mg showed that dabigatran trough concentration varied remarkably. Age, BMI and history of heart failure were identified as important covariates for dabigatran trough concentration. Dabigatran trough concentration (P = 0.002) and history of hypertension (P = 0.012) scores were identified as key factors for predicting the risk of bleeding events. Dabigatran trough concentration, affected by Age, BMI and history of heart failure, may serve as an independent risk factor for bleeding events in Chinese patients treated with DE for NVAF.
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http://dx.doi.org/10.1007/s00380-021-01974-0DOI Listing
October 2021

Advanced Molecular Imaging (MRI/MRS/H NMR) for Metabolic Information in Young Adults with Health Risk Obesity.

Life (Basel) 2021 Oct 1;11(10). Epub 2021 Oct 1.

Center of Radiation Research and Medical Imaging, Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.

Background: Obesity or being overweight is a medical condition of abnormal body fat accumulation which is associated with a higher risk of developing metabolic syndrome. The distinct body fat depots on specific parts of the anatomy have unique metabolic properties and different types of regional excessive fat distribution can be a disease hazard. The aim of this study was to identify the metabolome and molecular imaging phenotypes among a young adult population.

Methods: The amount and distribution of fat and lipid metabolites profile in the abdomen, liver, and calf muscles of 46 normal weight, 17 overweight, and 13 obese participants were acquired using MRI and MR spectroscopy (MRS), respectively. The serum metabolic profile was obtained using proton NMR spectroscopy. NMR spectra were integrated into seven integration regions, which reflect relative metabolites.

Results: A significant metabolic disorder symptom appeared in the overweight and obese group, and increased lipid deposition occurred in the abdomen, hepatocytes, and muscles that were statistically significant. Overall, the visceral fat depots had a marked influence on dyslipidemia biomarkers, blood triglyceride (r = 0.592, < 0.001), and high-density lipoprotein cholesterol (r = -0.484, < 0.001). Intrahepatocellular lipid was associated with diabetes predictors for hemoglobin (HbA1c%; r = 0.379, < 0.001) and for fasting blood sugar (r = 0.333, < 0.05). The lipid signals in serum triglyceride and glucose signals gave similar correspondence to biochemical lipid profiles.

Conclusions: This study proves the association between alteration in metabolome in young adults, which is the key population for early prevention of obesity and metabolic syndrome. This study suggests that dyslipidemia prevalence is influenced mainly by the visceral fat depot, and liver fat depot is a key determinant for glucose metabolism and hyperglycemia. Moreover, noninvasive advanced molecular imaging completely elucidated the impact of fat distribution on the anthropometric and laboratory parameters, especially indices of the metabolic syndrome biomarkers in young adults.
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http://dx.doi.org/10.3390/life11101035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541404PMC
October 2021

Sequence-Based Prediction of Plant Protein-Protein Interactions by Combining Discrete Sine Transformation With Rotation Forest.

Evol Bioinform Online 2021 12;17:11769343211050067. Epub 2021 Oct 12.

College of Information Engineering, Xijing University, Xi'an, China.

Protein-protein interactions (PPIs) in plants are essential for understanding the regulation of biological processes. Although high-throughput technologies have been widely used to identify PPIs, they are usually laborious, expensive, and suffer from high false-positive rates. Therefore, it is imperative to develop novel computational approaches as a supplement tool to detect PPIs in plants. In this work, we presented a method, namely DST-RoF, to identify PPIs in plants by combining an ensemble learning classifier-Rotation Forest (RoF) with discrete sine transformation (DST). Specifically, plant protein sequence is firstly converted into Position-Specific Scoring Matrix (PSSM). Then, the discrete sine transformation was employed to extract effective features for obtaining the evolutionary information of proteins. Finally, these optimal features were fed into the RoF classifier for training and prediction. When performed on the plant datasets Arabidopsis, Rice, and Maize, DST-RoF yielded high prediction accuracy of 82.95%, 88.82%, and 93.70%, respectively. To further evaluate the prediction ability of our approach, we compared it with 4 state-of-the-art classifiers and 3 different feature extraction methods. Comprehensive experimental results anticipated that our method is feasible and robust for predicting potential plant-protein interacted pairs.
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http://dx.doi.org/10.1177/11769343211050067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521741PMC
October 2021
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