Publications by authors named "Jie Ma"

1,727 Publications

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Spatial and temporal variation of heat islands in the main urban area of Zhengzhou under the two-way influence of urbanization and urban forestry.

PLoS One 2022 10;17(8):e0272626. Epub 2022 Aug 10.

School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, China.

Urban heat islands are major factors hindering the quality of present-day urban habitats. The ongoing acceleration of the worldwide urbanization process is leading to an exacerbation of the urban heat island effect; however, urban forestry can mitigate it. For a sustainable urban development, it is particularly important to evaluate the dual effect of both factors on the urban heat island phenomenon. In this study, we focused on Zhengzhou City (China), at the center of the Central Plains Forest City Cluster. The spatial and temporal evolutions of the local urban heat island and vegetation coverage were measured from Landsat 5 and Landsat 8 remote sensing images taken between 2006-2020 and the effects of urban construction and urban forestry on the urban heat island effect were evaluated. The results showed that, in the past 15 years, the high-temperature zone in the urban area of Zhengzhou City has gradually spread from its center to surrounding areas. Within the same period, the whole urban heat island has deteriorated and gradually improved: its area increased by 138.72 km2 between 2006-2014 and decreased by 135.66 km2 between 2014-2020. Notably, the development of vegetation coverage occurred consistently with the improvement of the heat island. A quantitative analysis of the relationship between urban construction, the urban forest, and the urban heat island has shown that factors like population density (representing urban construction), urban planning, and vegetation cover (representing the urban forest) all have an impact on the urban heat island. Based on the dynamic changes of the urban heat island in the urban area of Zhengzhou City between 2006-2020, we conclude that urban forest construction strategies are beginning to bear fruit. Overall, the findings of this study provide a theoretical basis for future urban construction and urban forest construction plans; moreover, they can support landscape pattern optimization and urban heat island mitigation.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0272626PLOS
August 2022

αSMA-Cre-mediated Ogt deletion leads to heart failure and vascular smooth muscle cell dysfunction in mice.

Biochem Biophys Res Commun 2022 Jul 31;625:31-37. Epub 2022 Jul 31.

School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan, China. Electronic address:

Dilated cardiomyopathy, a type of heart muscle disease defined by the presence of left ventricular dilatation and contractile dysfunction, is an important cause of sudden cardiac death and heart failure. O-GlcNAcylation is an important post-translational modification of proteins by the addition of O-GlcNAc moieties at serine or threonine residues. Several studies have shown that proper control of O-GlcNAcylation is required for maintaining physiological function of heart by using Ogt (O-GlcNAc transferase) cardiomyocyte-specific knockout mouse models. In this study, we generated a new mouse model (αSMA-Ogt KO) in which Ogt was deleted in both cardiomyocytes and smooth muscle cells by crossing Ogt floxed mice with αSMA-Cre mice. αSMA-Cre-mediated Ogt deletion in mice led to severe postnatal lethality; the survived mice were smaller than control mice, had dilated hearts, and showed observable signs of heart failure. Moreover, the αSMA-Ogt KO heart had more apoptotic cells and fibrosis. The arteries of αSMA-Ogt KO mice exhibited significantly reduced expression of contractile genes and a trend towards arterial stiffness. In conclusion, our data emphasize the importance of OGT in maintaining normal heart function and reveal a novel role of OGT in regulating arterial contractility.
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http://dx.doi.org/10.1016/j.bbrc.2022.07.106DOI Listing
July 2022

Correction: Cold atmospheric plasma induces GSDME-dependent pyroptotic signaling pathway via ROS generation in tumor cells.

Cell Death Dis 2022 Aug 8;13(8):690. Epub 2022 Aug 8.

Anhui Province Key Laboratory of Medical Physics and Technology/Center of Medical Physics and Technology, Hefei Institutes of Physical Sciences, Chinese Academy of Sciences, Hefei, Anhui, China.

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http://dx.doi.org/10.1038/s41419-022-05143-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359992PMC
August 2022

Reference intervals of 14 biochemical markers for children and adolescence in China: the PRINCE study.

Clin Chem Lab Med 2022 Aug 8. Epub 2022 Aug 8.

National Center for Children's Health, Beijing Children's Hospital, Capital Medical University, Beijing, P.R. China.

Objectives: The Pediatric Reference Intervals in China (PRINCE) was initiated to establish the reference intervals (RIs) of Chinese children, as well as to make it possible to compare the variability of biochemical markers among countries internationally.

Methods: Healthy participants, aged up to 20 years, from 11 provinces across China, were enrolled in PRINCE and according to a standard screening procedure, that included a questionnaire survey, physical examinations and laboratory tests. Fasting venous blood specimens were collected. All serum specimens were analyzed with Cobas C702 in the center laboratory, i.e. clinical laboratory of Beijing Children's Hospital, with certified qualification (ISO15189). The nonparametric method recommended by Clinical Laboratory Standards Institute guidelines, was used to calculate the age- and sex-specified RIs.

Results: Among the 15,150 participants enrolled, 12,352 children (6,093 males and 6,259 females) were included to calculate RIs. The RIs for total protein, albumin, globulin, calcium, phosphate, potassium, sodium, chlorine, alkaline phosphatase, γ-glutamyl transpeptadase, alanine aminotransferase, aspartate aminotransferase, creatinine and urea were established by age- or sex-partitions. Most biochemical markers displayed larger variability and higher dispersion during the periods between 28 days and 1 year old, and included 4-6 age partitions commonly during 1 to <20 years old. In addition, differences of RIs between sexes usually occurs around the initiation of puberty at 12-13 years old.

Conclusions: The age- and sex-specified RIs of 14 biochemical markers in PRINCE study can provide a solid reference, which will be transferred into relevant RIs for other clinical laboratory's platforms according to the CLSI guidelines.
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http://dx.doi.org/10.1515/cclm-2022-0299DOI Listing
August 2022

Electrochemical pretreatment enhancing co-fermentation of waste activated sludge and food waste into volatile fatty acids: Performance, microbial community dynamics and metabolism.

Bioresour Technol 2022 Aug 3;361:127736. Epub 2022 Aug 3.

School of Environmental Science and Engineering, Huazhong University of Science and Technology (HUST), Key Laboratory of Water and Wastewater Treatment (HUST), MOHURD, Wuhan 430074, China. Electronic address:

Waste activated sludge (WAS) has low biodegradability that restricts acidogenic fermentation (AF), thereby limiting the high-value volatile fatty acids (VFAs) production. This study investigated an alternative electrochemical pretreatment (EPT) approach that can facilitate AF of WAS and food waste (FW) and therefore enhance VFAs production. The results showed through introducing 50 % volatile solid basis of FW (containing massive chloride) into WAS, a 60-min EPT produced reactive chlorine species (RCS), which diffused into WAS-FW inner layers resulting in cell lysis, therefore significantly promoted and accelerated WAS-FW disintegration, contributing to more soluble and biodegradable dissolved organic matter (DOM). Then during the subsequent 15-day acidogenic co-fermentation (Co-AF), the residual RCS (approximate 5 mg Cl/L) also caused acidogenic bacteria (including Prevotella_7, Lactobacillus and Veillonella) gradually outcompeted methanogens due to their different tolerance to residual RCS. Consequently, the maximum VFAs yield of the WAS-FW Co-AF with EPT was 40.8 % higher than WAS-AF without EPT.
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http://dx.doi.org/10.1016/j.biortech.2022.127736DOI Listing
August 2022

Editorial: Effects of economic shocks on human behavior, mental life and the environment: Implications for the post-COVID-19 crisis era.

Front Psychol 2022 19;13:922875. Epub 2022 Jul 19.

Newcastle Business School, Northumbria University, Newcastle upon Tyne, United Kingdom.

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http://dx.doi.org/10.3389/fpsyg.2022.922875DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345198PMC
July 2022

Adsorption of di (2-ethylhexyl) phthalate (DEHP) to microplastics in seawater: a comparison between pristine and aged particles.

Bull Environ Contam Toxicol 2022 Aug 3. Epub 2022 Aug 3.

Marine Environmental Monitoring Center of Guangxi, 536000, Beihai, P R China.

Microplastics (MPs) are a widely distributed pollutant and have been attracting global attention. The increasing abundance of MPs in marine environments has raised concern about their adverse effects on marine organisms and influence on the fate of contaminants in seawater. In this study, we investigated the effects of natural aging on the adsorption of di (2-ethylhexyl) phthalate (DEHP), one of the most widely used phthalic acid esters (PAEs), in two types of MPs (polyethylene and polystyrene). Biofilm was observed on the surface of MPs after 3-month exposure in seawater. Atomic force microscopy revealed there were significant physical changes in the MPs after aging. Aging in coastal seawater for 3 months significantly reduced the MPs' surface roughness and adhesion, and increased the Young's modulus at the same time. Adsorption isotherms of DEHP indicated that aged MPs had stronger binding capacity of the organic contaminant than pristine MPs. Our data shed some light on the biogeochemical role of MPs in marine environments.
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http://dx.doi.org/10.1007/s00128-022-03570-yDOI Listing
August 2022

Genomic Profiling Reveals Novel Predictive Biomarkers for Chemo-Radiotherapy Efficacy and Thoracic Toxicity in Non-Small-Cell Lung Cancer.

Front Oncol 2022 14;12:928605. Epub 2022 Jul 14.

Department of Radiation Oncology, Shandong University Cancer Center, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.

Chemo-radiotherapy (CRT) remains the main treatment modality for non-small-cell lung cancer (NSCLC). However, its clinical efficacy is largely limited by individual variations in radio-sensitivity and radiotherapy-associated toxicity. There is an urgent need to identify genetic determinants that can explain patients' likelihood to develop recurrence and radiotherapy-associated toxicity following CRT. In this study, we performed comprehensive genomic profiling, using a 474-cancer- and radiotherapy-related gene panel, on pretreatment biopsy samples from patients with unresectable stage III NSCLCs who underwent definitive CRT. Patients' baseline clinical characteristics and genomic features, including tumor genetic, genomic and molecular pathway alterations, as well as single nucleotide polymorphisms (SNPs), were correlated with progression-free survival (PFS), overall survival (OS), and radiotherapy-associated pneumonitis and/or esophagitis development after CRT. A total of 122 patients were enrolled between 2014 and 2019, with 84 (69%) squamous cell carcinomas and 38 (31%) adenocarcinomas. Genetic analysis confirmed the association between the KEAP1-NRF2 pathway gene alterations and unfavorable survival outcome, and revealed alterations in family genes, , , and as potential novel and independent risk factors of poor post-CRT survival. Combined analysis of such alterations led to improved stratification of the risk populations. In addition, patients with activating mutations or any oncogenic driver mutations exhibited improved OS. On the other hand, we also identified genetic markers in relation to radiotherapy-associated thoracic toxicity. SNPs in the DNA repair-associated (rs3835) and (rs25487) were associated with an increased risk of high-grade esophagitis and pneumonitis respectively. (rs1801133) and (rs1800566) were additional risk alleles related to higher susceptibility to pneumonitis and esophagitis overall. Moreover, through their roles in genome integrity and replicative fidelity, somatic alterations in and might also serve as risk predictors of high-grade pneumonitis and esophagitis. Taken together, leveraging targeted next-generating sequencing, we identified a set of novel clinically applicable biomarkers that might enable prediction of survival outcomes and risk of radiotherapy-associated thoracic toxicities. Our findings highlight the value of pre-treatment genetic testing to better inform CRT outcomes and clinical actions in stage III unresectable NSCLCs.
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http://dx.doi.org/10.3389/fonc.2022.928605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329611PMC
July 2022

Current and Future Potential of Shellfish and Algae Mariculture Carbon Sinks in China.

Int J Environ Res Public Health 2022 Jul 21;19(14). Epub 2022 Jul 21.

College of Environment, Hohai University, Nanjing 210024, China.

Shellfish and algae mariculture make up an important part of the marine fishery carbon sink. Carbon sink research is necessary to ensure China achieves its goal of carbon neutrality. This study used the material quality assessment method to estimate the carbon sink capacity of shellfish and algae. Product value, carbon storage value, and oxygen release value were used to calculate the economic value of shellfish and algae carbon sequestration. The results showed that the annual average shellfish and algae carbon sink in China was 1.10 million tons from 2003 to 2019, of which shellfish accounted for 91.63%, wherein , , and were the main contributors. The annual average economic value of China's shellfish and algae carbon sequestration was USD 71,303.56 million, and the product value was the main contributor, accounting for 99.11%. The carbon sink conversion ratios of shellfish and algae were 8.37% and 5.20%, respectively, thus making shellfish the aquaculture species with the strongest carbon sink capacity and the greatest carbon sink potential. The estimated growth rate in the shellfish and algae removable carbon sink was 33,900 tons/year in China, but this trend was uncertain. The capacity for carbon sequestration and exchange by aquaculture can be improved by expanding breeding space, promoting multi-level comprehensive breeding modes, and marine artificial upwelling projects.
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http://dx.doi.org/10.3390/ijerph19148873DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322719PMC
July 2022

Integrating Activity-Guided Strategy and Fingerprint Analysis to Target Potent Cytotoxic Brefeldin A from a Fungal Library of the Medicinal Mangrove .

Mar Drugs 2022 Jun 29;20(7). Epub 2022 Jun 29.

Key Laboratory of Marine Drugs, The Ministry of Education of China, School of Medicine and Pharmacy, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.

Mangrove-associated fungi are rich sources of novel and bioactive compounds. A total of 102 fungal strains were isolated from the medicinal mangrove collected from the South China Sea. Eighty-four independent culturable isolates were identified using a combination of morphological characteristics and internal transcribed spacer (ITS) sequence analyses, of which thirty-seven strains were selected for phylogenetic analysis. The identified fungi belonged to 22 genera within seven taxonomic orders of one phyla, of which four genera , , , and were first isolated from mangroves. The cytotoxic activity of organic extracts from 55 identified fungi was evaluated against human lung cancer cell lines (A-549), human cervical carcinoma cell lines (HeLa), human hepatoma cells (HepG2), and human acute lymphoblastic leukemia cell lines (Jurkat). The crude extracts of 31 fungi (56.4%) displayed strong cytotoxicity at the concentration of 50 μg/mL. Furthermore, the fungus sp. (HS-N-27) still showed strong cytotoxic activity at the concentration of 25 µg/mL. Integrating cytotoxic activity-guided strategy and fingerprint analysis, a well-known natural Golgi-disruptor and Arf-GEFs inhibitor, brefeldin A, was isolated from the target active strain HS-N-27. It displayed potential activity against A549, HeLa and HepG2 cell lines with the IC values of 101.2, 171.9 and 239.1 nM, respectively. Therefore, combining activity-guided strategy with fingerprint analysis as a discovery tool will be implemented as a systematic strategy for quick discovery of active compounds.
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http://dx.doi.org/10.3390/md20070432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315649PMC
June 2022

Identification of a Prognostic Transcriptome Signature for Hepatocellular Carcinoma with Lymph Node Metastasis.

Oxid Med Cell Longev 2022 6;2022:7291406. Epub 2022 Jul 6.

Department of Radiation Oncology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.

Hepatocellular carcinoma (HCC) is one of the most aggressive malignant tumors, and the prognosis of HCC patients with lymph node metastasis (LNM) is poor. However, robust biomarkers for predicting the prognosis of HCC LNM are still lacking. This study used weighted gene coexpression network analysis of GSE28248 ( = 80) microarray data to identify gene modules associated with HCC LNM and validated in GSE40367 dataset ( = 18). The prognosis-related genes in the HCC LNM module were further screened based on the prognostic curves of 371 HCC samples from TCGA. We finally developed a prognostic signature, PSG-30, as a prognostic-related biomarker in HCC LNM. The HCC subtypes identified by PSG-30-based consensus clustering analysis showed significant differences in prognosis, clinicopathological stage, m6A modification, ferroptosis activation, and immune characteristics. In addition, was selected by regression model as an independent risk factor affecting the prognosis of HCC patients with LNM, and its expression was significantly positively correlated with tumor mutational burden and microsatellite instability in high-risk subtypes. Patients with high expression had a better prognosis in the immune checkpoint inhibitor-treated cohorts but had a poor prognosis in the HCC sorafenib-treated group. The association of high expression with LNM in breast cancer (BRCA) and cholangiocarcinoma and its prognostic effect in BRCA LNM cases suggest the value of at the pancancer level. In conclusion, PSG-30 can effectively identify HCC LNM population with poor prognosis, and high-risk patients with high expression may be more suitable for immunotherapy.
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http://dx.doi.org/10.1155/2022/7291406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279092PMC
July 2022

Glycolysis in Innate Immune Cells Contributes to Autoimmunity.

Front Immunol 2022 1;13:920029. Epub 2022 Jul 1.

Department of Rheumatology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China.

Autoimmune diseases (AIDs) refer to connective tissue inflammation caused by aberrant autoantibodies resulting from dysfunctional immune surveillance. Most of the current treatments for AIDs use non-selective immunosuppressive agents. Although these therapies successfully control the disease process, patients experience significant side effects, particularly an increased risk of infection. There is a great need to study the pathogenesis of AIDs to facilitate the development of selective inhibitors for inflammatory signaling to overcome the limitations of traditional therapies. Immune cells alter their predominant metabolic profile from mitochondrial respiration to glycolysis in AIDs. This metabolic reprogramming, known to occur in adaptive immune cells, i.e., B and T lymphocytes, is critical to the pathogenesis of connective tissue inflammation. At the cellular level, this metabolic switch involves multiple signaling molecules, including serine-threonine protein kinase, mammalian target of rapamycin, and phosphoinositide 3-kinase. Although glycolysis is less efficient than mitochondrial respiration in terms of ATP production, immune cells can promote disease progression by enhancing glycolysis to satisfy cellular functions. Recent studies have shown that active glycolytic metabolism may also account for the cellular physiology of innate immune cells in AIDs. However, the mechanism by which glycolysis affects innate immunity and participates in the pathogenesis of AIDs remains to be elucidated. Therefore, we reviewed the molecular mechanisms, including key enzymes, signaling pathways, and inflammatory factors, that could explain the relationship between glycolysis and the pro-inflammatory phenotype of innate immune cells such as neutrophils, macrophages, and dendritic cells. Additionally, we summarize the impact of glycolysis on the pathophysiological processes of AIDs, including systemic lupus erythematosus, rheumatoid arthritis, vasculitis, and ankylosing spondylitis, and discuss potential therapeutic targets. The discovery that immune cell metabolism characterized by glycolysis may regulate inflammation broadens the avenues for treating AIDs by modulating immune cell metabolism.
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http://dx.doi.org/10.3389/fimmu.2022.920029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284233PMC
July 2022

Editorial: Sex Determination and Developmental Mechanism of Crustacean and Shellfish.

Front Endocrinol (Lausanne) 2022 28;13:940144. Epub 2022 Jun 28.

Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.

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http://dx.doi.org/10.3389/fendo.2022.940144DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275810PMC
June 2022

A super pan-genomic landscape of rice.

Cell Res 2022 Jul 12. Epub 2022 Jul 12.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang, China.

Pan-genomes from large natural populations can capture genetic diversity and reveal genomic complexity. Using de novo long-read assembly, we generated a graph-based super pan-genome of rice consisting of a 251-accession panel comprising both cultivated and wild species of Asian and African rice. Our pan-genome reveals extensive structural variations (SVs) and gene presence/absence variations. Additionally, our pan-genome enables the accurate identification of nucleotide-binding leucine-rich repeat genes and characterization of their inter- and intraspecific diversity. Moreover, we uncovered grain weight-associated SVs which specify traits by affecting the expression of their nearby genes. We characterized genetic variants associated with submergence tolerance, seed shattering and plant architecture and found independent selection for a common set of genes that drove adaptation and domestication in Asian and African rice. This super pan-genome facilitates pinpointing of lineage-specific haplotypes for trait-associated genes and provides insights into the evolutionary events that have shaped the genomic architecture of various rice species.
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http://dx.doi.org/10.1038/s41422-022-00685-zDOI Listing
July 2022

Spatially resolved proteomic map shows that extracellular matrix regulates epidermal growth.

Nat Commun 2022 Jul 11;13(1):4012. Epub 2022 Jul 11.

Stem Cell and Regenerative Medicine Lab, State Key Laboratory of Complex Severe and Rare Diseases, Translational Medicine Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Human skin comprises stratified squamous epithelium and dermis with various stromal cells and the extracellular matrix (ECM). The basement membrane (BM), a thin layer at the top of the dermis, serves as a unique niche for determining the fate of epidermal stem cells (EpSCs) by transmitting physical and biochemical signals to establish epidermal cell polarity and maintain the hierarchical structure and function of skin tissue. However, how stem cell niches maintain tissue homeostasis and control wound healing by regulating the behavior of EpSCs is still not completely understood. In this study, a hierarchical skin proteome map is constructed using spatial quantitative proteomics combined with decellularization, laser capture microdissection, and mass spectrometry. The specific functions of different structures of normal native skin tissues or tissues with a dermatologic disease are analyzed in situ. Transforming growth factor-beta (TGFβ)-induced protein ig-h3 (TGFBI), an ECM glycoprotein, in the BM is identified that could enhance the growth and function of EpSCs and promote wound healing. Our results provide insights into the way in which ECM proteins facilitate the growth and function of EpSCs as part of an important niche. The results may benefit the clinical treatment of skin ulcers or diseases with refractory lesions that involve epidermal cell dysfunction and re-epithelialization block in the future.
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http://dx.doi.org/10.1038/s41467-022-31659-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273758PMC
July 2022

Pulmonary Microbial Composition in Sepsis-Induced Acute Respiratory Distress Syndrome.

Front Mol Biosci 2022 23;9:862570. Epub 2022 Jun 23.

Clinical Experimental Center, Jiangmen Key Laboratory of Clinical Biobanks and Translational Research, Jiangmen Central Hospital, Jiangmen, China.

Acute respiratory distress syndrome (ARDS) is an unresolved challenge in the field of respiratory and critical care, and the changes in the lung microbiome during the development of ARDS and their clinical diagnostic value remain unclear. This study aimed to explore the role of the lung microbiome in disease progression in patients with sepsis-induced ARDS and potential therapeutic targets. Patients with ARDS were divided into two groups according to the initial site of infection, intrapulmonary infection (ARDSp, 111 cases) and extrapulmonary infection (ARDSexp, 45 cases), and a total of 28 patients with mild pulmonary infections were enrolled as the control group. In this study, we sequenced the DNA in the bronchoalveolar lavage fluid collected from patients using metagenomic next-generation sequencing (mNGS) to analyze the changes in the lung microbiome in patients with different infectious site and prognosis and before and after antibiotic treatment. The Shannon-Wiener index indicated a statistically significant reduction in microbial diversity in the ARDSp group compared with the ARDSexp and control groups. The ARDSp group was characterized by a reduction in microbiome diversity, mainly in the normal microbes of the lung, whereas the ARDSexp group was characterized by an increase in microbiome diversity, mainly in conditionally pathogenic bacteria and intestinal microbes. Further analysis showed that an increase in is a potential risk factor for death in ARDSexp. An increase in , , , enteric microbes, or conditional pathogens may be risk factors for death in ARDSp. In contrast, may be a protective factor in ARDSp. Different initial sites of infection and prognoses are likely to affect the composition and diversity of the pulmonary microbiome in patients with septic ARDS. This study provides insights into disease development and exploration of potential therapeutic targets.
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http://dx.doi.org/10.3389/fmolb.2022.862570DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262094PMC
June 2022

Multiplex immunohistochemistry defines the tumor immune microenvironment and immunotherapeutic outcome in CLDN18.2-positive gastric cancer.

BMC Med 2022 07 11;20(1):223. Epub 2022 Jul 11.

Department of Gastrointestinal Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, China.

Background: The FAST study identified claudin-18 (CLDN18.2) as a promising novel therapeutic target for gastric cancer (GC). However, the tumor immune microenvironment and clinicopathological features of CLDN18.2-positive GC are unclear, making it difficult to develop and optimize CLDN18.2-targeted treatments.

Methods: This study included 80 GC patients, 60 of whom received anti-PD-1/PD-L1 treatment. CD4/CD8/CD20/CD66b/CD68/CD163/PD-1/PD-L1/TIM-3/LAG-3/FoxP3/CTLA-4/HLA-DR/STING, and CLDN18.2 were labeled using multiplex immunohistochemistry (m-IHC) to decipher the rate and spatial distribution of T cells, B cells, macrophages, and neutrophils in formalin-fixed, paraffin-embedded tumor tissues isolated from these patients. Tumor immune-microenvironmental features and patient survival stratified by CLDN18.2 expression were analyzed using two independent-sample t-tests and log-rank tests, respectively.

Results: We considered moderate-to-strong CLDN18.2 expression ≥ 40% of tumor cells as the cut-off for positivity. The proportion of CD8PD-1, CD8LAG-3, and CD8TIM-3 T cells was significantly higher in CLDN18.2-positive tumors than in negative tumors (0.039 vs. 0.026, P = 0.009; 0.050 vs.0.035, P = 0.024; 0.045 vs. 0.032, P = 0.038, respectively). In addition, the number of neutrophils (CD66b) was higher in the CLDN18.2-positive group than in the negative group (0.081 vs. 0.055, P = 0.031, respectively), while the rates of M1 (CD68CD163HLA-DR), M2 macrophages (CD68CD163HLA-DR), and B cells (CD20) were comparable between the CLDN18.2-positive and negative groups. The average numbers of CD8PD-1, CD8LAG-3, and CD8TIM-3T cells surrounding tumor cells within a 20-μm range were higher in CLDN18.2-positive tumors than in the CLDN18.2-negative tumors (0.16 vs. 0.09, P = 0.011; 0.20 vs. 0.12, P = 0.029; 0.18 vs. 0.12, P = 0.047, respectively). In addition, in the CLDN18.2-positive group, tumor cells surrounded by CD8PD-1, CD8LAG-3 T cells, or M1 macrophages within a 20-μm range accounted for a higher proportion of all tumor cells than those in the CLDN18.2-negative group (10.79% vs. 6.60%, P = 0.015; 12.68% vs. 8.70%, P = 0.049; 9.08% vs. 6.56%, P = 0.033, respectively). These findings suggest that CLDN18.2-positive GC harbors complex immune-microenvironmental features. Additionally, CLDN18.2-positive group had shorter OS and irOS than CLDN18.2-negative group (median OS: 23.33 vs.36.6 months, P < 0.001; median irOS: 10.03 vs. 20.13 months, P = 0.044, respectively).

Conclusions: CLDN18.2-positive GC displayed unique immune-microenvironmental characteristics, which is of great significance for the development of CLDN18.2-targeted therapies. However, the impact of CLDN18.2-related microenvironmental features on prognosis requires further investigation.
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http://dx.doi.org/10.1186/s12916-022-02421-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9272556PMC
July 2022

Antimicrobial Resistance and Molecular Characterization of Gene Cassettes from class 1 Integrons in Carbapenem-resistant Escherichia coli strains.

Microb Pathog 2022 Jul 6;170:105669. Epub 2022 Jul 6.

Clinical Laboratory of Weifang People's Hospital, 151 Guangwen Street, Weifang, Shandong Province, 261041, China. Electronic address:

Objective: To investigate the distribution of class 1 integrons and their variable regional molecular characteristics, as well as the diversity of promoter and drug sensitivity of CR-Eco (carbapenem-resistant E. coli) strains.

Method: A total of 117 CR-Eco strains, collected between 2012.01 and 2019.12, underwent fully automated bacterial identification and sensitization using VITEK-2 Compact and supplemented by K-B assay. PCR was employed to screen for class 1 integrase genes and integron variable regions, while the promoter type and variable region gene cassette characteristics were determined by sequencing analysis.

Results: The positive rate of the class 1 integron of the CR-Eco strains was 83.70% (92/117) herein. Moreover, class 1 integrase-positive strains exhibited statistically significant resistance to aztreonam, ceftazidime, ciprofloxacin, ceftriaxone, gentamicin, meropenem, and trimethoprim-sulfamethoxazole compared to integron-negative strains (P < 0.05). Variable regions were observed in 77 of the 92 class 1 integrase-positive strains. In addition, seven gene cassettes were detected, namely dfrA17-aadA5, aadA22, dfrA12-aadA2, dfrA12, dfrA17, dfrA27 and aadA. Finally, five types of class 1 integron variable region promoters were identified in those 77 strains, including PcW, PcH1, PcW, PcH1, and P2, which were detected in 48, 18, 8, 2, and 1 strains, respectively.

Conclusion: The primary integrator variable region gene cassettes of this class were dfrA and aadA. The integron-positive strains displayed simultaneous high resistance to multiple antimicrobial drugs. The integrator variable region promoters of the CR-Eco strains are primarily weak and can potentially form and spread drug resistance.
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http://dx.doi.org/10.1016/j.micpath.2022.105669DOI Listing
July 2022

Variation in the Main Health-Promoting Compounds and Antioxidant Activity of Different Edible Parts of Purple Flowering Stalks ( var. ) and Green Flowering Stalks ( var. ).

Plants (Basel) 2022 Jun 23;11(13). Epub 2022 Jun 23.

College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China.

Purple flowering stalks and green flowering stalks of are widely cultivated in the middle and upper reaches of the Yangtze River. Here, concentrations of the main health-promoting compounds and antioxidant capacity levels were characterized in different parts (leaves, peel, flesh, and inflorescences) of purple and green flowering stalks. There were significant differences in the concentrations of health-promoting compounds between the two variants; the concentrations of pigments, especially anthocyanidins, and gluconapin, were significantly higher in purple flowering stalks than in green flowering stalks, and the progoitrin content was significantly higher in green flowering stalks than in purple flowering stalks. The leaves were judged to be the most nutritional edible part because they had the highest concentrations of pigments, ascorbic acid, proanthocyanidins, flavonoids, and total phenolics. Antioxidant capacity was also highest in the leaves, and it was positively correlated with the concentration of health-promoting compounds. Purple flowering stalks and green flowering stalks were found to be rich in health-promoting compounds, especially glucosinolates. Overall, our findings indicate that consumption of the leaves and peel would provide the most health benefits. Some suggestions are provided regarding the processing and utilization of these edible components.
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http://dx.doi.org/10.3390/plants11131664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269110PMC
June 2022

MiR-487b suppressed inflammation and neuronal apoptosis in spinal cord injury by targeted Ifitm3.

Metab Brain Dis 2022 Jul 8. Epub 2022 Jul 8.

Department of Orthopedics, Changhai Hospital, Naval Medical University, 168 Changhai Road, Shanghai, 200433, People's Republic of China.

Spinal cord injury (SCI) was a serious nerve injury, which involves complex genetic changes. This paper was intended to investigate the function and mechanism of differentially expressed genes in SCI. The three datasets GSE92657, GSE93561 and GSE189070 of SCI from GEO database were used to identify differentially expressed genes (DEGs). We identified the common DEGs in the three datasets GSE92657, GSE93561 and GSE189070 of SCI from GEO database. Next, a protein-protein interaction (PPI) network of DEGs was constructed. Subsequently, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that DEGs were significantly enriched in immune response, inflammatory response. The expression level of immune-related genes (Arg1, Ccl12, Ccl2, Ifitm2, Ifitm3, and et al.) at different time points of SCI were analyzed in GSE189070 dataset. Next, differentially expressed miRNAs (DE-miRNAs) were identified in SCI compared with normal based on GSE158194 database. DE-miRNA and targeted immune-related genes were predicted by miRwalk, including miR-487b-5p targeted Ifitm3, miR-3072-5p targeted Ccl3, and et al. What's more, the miR-487b was identified and verified to be down-regulated in Lipopolysaccharide (LPS)-induced BV-2 cell model. Further, the miR-487b inhibited cell inflammation and apoptosis in LPS-induced BV2 cell by targeted Ifitm3. For the first time, our results revealed that miR-487b may play an important regulatory role in SCI by targeted Ifitm3 and provide further evidence for SCI research.
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http://dx.doi.org/10.1007/s11011-022-01015-3DOI Listing
July 2022

Risk of bias of prognostic models developed using machine learning: a systematic review in oncology.

Diagn Progn Res 2022 Jul 7;6(1):13. Epub 2022 Jul 7.

Centre for Statistics in Medicine, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, OX3 7LD, UK.

Background: Prognostic models are used widely in the oncology domain to guide medical decision-making. Little is known about the risk of bias of prognostic models developed using machine learning and the barriers to their clinical uptake in the oncology domain.

Methods: We conducted a systematic review and searched MEDLINE and EMBASE databases for oncology-related studies developing a prognostic model using machine learning methods published between 01/01/2019 and 05/09/2019. The primary outcome was risk of bias, judged using the Prediction model Risk Of Bias ASsessment Tool (PROBAST). We described risk of bias overall and for each domain, by development and validation analyses separately.

Results: We included 62 publications (48 development-only; 14 development with validation). 152 models were developed across all publications and 37 models were validated. 84% (95% CI: 77 to 89) of developed models and 51% (95% CI: 35 to 67) of validated models were at overall high risk of bias. Bias introduced in the analysis was the largest contributor to the overall risk of bias judgement for model development and validation. 123 (81%, 95% CI: 73.8 to 86.4) developed models and 19 (51%, 95% CI: 35.1 to 67.3) validated models were at high risk of bias due to their analysis, mostly due to shortcomings in the analysis including insufficient sample size and split-sample internal validation.

Conclusions: The quality of machine learning based prognostic models in the oncology domain is poor and most models have a high risk of bias, contraindicating their use in clinical practice. Adherence to better standards is urgently needed, with a focus on sample size estimation and analysis methods, to improve the quality of these models.
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http://dx.doi.org/10.1186/s41512-022-00126-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9261114PMC
July 2022

[Nephrotic Syndrome Caused by AA Amyloidosis Secondary to Unicentric Castleman's Disease:Report of One Case].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2022 Jun;44(3):540-544

Department of Nephrology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.

AA amyloidosis is a rare systemic complication caused commonly by chronic inflammatory arthritis,periodic fever disease,vasculitis,tumors,etc.Castleman's disease is an uncommon cause of AA amyloidosis.Here,we reported a case of unicentric Castleman's disease-induced AA amyloidosis with nephrotic syndrome as the main manifestation.The laboratory examination showed elevated levels of inflammatory indicators.We summarized the clinical manifestations,diagnosis,and therapy of this case,aiming to facilitate the management of patients with unknown reasons of renal amyloidosis.
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http://dx.doi.org/10.3881/j.issn.1000-503X.14104DOI Listing
June 2022

Correction to: Calculating glenoid bone loss based on glenoid height using ipsilateral three-dimensional computed tomography.

Knee Surg Sports Traumatol Arthrosc 2022 Jul 6. Epub 2022 Jul 6.

Division of Orthopaedic Surgery, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada.

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http://dx.doi.org/10.1007/s00167-022-07055-7DOI Listing
July 2022

Single-Cell Transcriptome Analysis Reveals Changes of Tumor Immune Microenvironment in Oral Squamous Cell Carcinoma After Chemotherapy.

Front Cell Dev Biol 2022 17;10:914120. Epub 2022 Jun 17.

Department of Oral Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Induction chemotherapy in oral squamous cell carcinoma is a controversial issue in clinical practice. To investigate the evolution of cancer cells and tumor microenvironment (TME) response to chemotherapy in oral squamous cell carcinoma, single-cell transcriptome analysis was performed in a post-chemotherapy squamous cell carcinoma located in oral cavity. The main cell types were identified based on gene expression patterns determined using dimensionality reduction and unsupervised cell clustering. Non-negative matrix factorization clustering of the gene expression of Cancer-associated fibroblasts (CAFs) and macrophages was performed. Kyoto Encyclopedia of Genes and Genomes pathway analyses and gene set enrichment analysis were performed to explore significant functional pathways. CellPhoneDB and NicheNet were used to detect the intercellular communication between cell types. CAFs were divided into "inflammatory CAFs," "antigen-presenting CAFs" and "myofibroblastic CAFs." Three classic subgroups of tumor-associated macrophages (TAMs) were detected, namely C1Q (+), FCN1 (+) and SPP1(+) TAMs. The inflammatory cytokine expression is elevated, and several molecular pathways, such as PI3K/Akt/mTORC1, TNF-α NFκB, TGF-β, IL-6/JAK2/STAT3 and CXCL12/CXCR4 axis associated with epithelial-mesenchymal transition were enriched in TME. Also, CD74-MIF/COPA/APP interactions were expressed in TME of oral squamous cell carcinoma after chemotherapy. The results revealed the characteristics of TME in post-chemotherapy oral squamous cell carcinoma at single-cell transcriptome level, providing new insights and clues for further investigation.
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http://dx.doi.org/10.3389/fcell.2022.914120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247458PMC
June 2022

Combining GWAS, Genome-Wide Domestication and a Transcriptomic Analysis Reveals the Loci and Natural Alleles of Salt Tolerance in Rice ( L.).

Front Plant Sci 2022 16;13:912637. Epub 2022 Jun 16.

State Key Laboratory for Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China.

Soil salinity poses a serious threat to the sustainable production of rice ( L.) throughout the world. Thus, the detection of loci and alleles responsible for salt tolerance is fundamental to accelerating the improvement of rice and producing the resilient varieties that will ensure future harvests. In this study, we collected a set of 191 mini-core rice populations from around the world, evaluated their salt tolerance based on plant growth and development phenotypes at the seedling stage, and divided a standard evaluation score (SES) of visual salt injury into five different grades. We used ∼3.82 million single nucleotide polymorphisms (SNPs) to identify 155 significant SNPs and 275 genes associated with salt sensitivity based on a genome-wide association study (GWAS) of SES. In particular, two candidate genes, and , were associated with salt tolerance, and was co-detected in the entire GWAS of all the panels and . Additionally, we investigated the transcriptional changes in cultivars 93-11 and PA64s under normal and salinity stress conditions and found 517 co-upregulated and 223 co-downregulated genes. These differentially expressed genes (DEGs) were highly enriched in "response to chemical" and "stress" based on the gene ontology enrichment analysis. Notably, 30 candidate genes that were associated with the salt tolerance analysis were obtained by integrating GWAS and transcriptomic DEG analyses, including 13 cloned genes that had no reports of tolerance to salt and 17 candidate genes whose functions were unknown. To further explore these genes and their alleles, we performed haplotype analysis, genome-wide domestication detection, and transcriptome analysis to breed improved varieties. This data and the genetic resources provided will be valuable for the development of salt tolerant rice varieties.
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http://dx.doi.org/10.3389/fpls.2022.912637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9248812PMC
June 2022

Effect of light polarization on plasmon-induced charge transfer.

J Chem Phys 2022 Jun;156(24):244704

Beijing Computational Science Research Center, Beijing 100193, China.

Plasmonic nanoclusters can strongly absorb light energy and generate hot carriers, which have great potentials in photovoltaic and photocatalytic applications. A vital step for those plasmonic applications is the charge transfer at the metal-semiconductor interface. The effect of the light polarization on the charge transfer has not been theoretically investigated so far. Here, we take the Ag-TiO system as a model system to study the polarization effect using time-dependent density functional theory simulations. We find that the charge transfer is sensitive to the light polarization, which has its origin in the polarization-dependent hot carrier distributions. For the linearly polarized light, it shows a sine-square dependence on the polar angle, indicating that the charge transfer response to the linear polarization can be decomposed into components perpendicular and parallel to the interface. We also find that there exists directional charge transfer with a circular light polarization. Our results demonstrate that the light polarization can significantly affect the charge transfer behavior and, thus, offer a new degree of freedom to manipulate the plasmonic applications.
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http://dx.doi.org/10.1063/5.0094444DOI Listing
June 2022

Evolved Biosensor with High Sensitivity and Specificity for Measuring Cadmium in Actual Environmental Samples.

Environ Sci Technol 2022 07 28;56(14):10062-10071. Epub 2022 Jun 28.

Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.

Bacterial biosensors have great potential in contaminant detection for sensitivity, specificity, cost-effectiveness, and easy operation. However, the existing cadmium-responsive bacterial biosensors cannot meet the real-world detection requirements due to lack of sensitivity, specificity, and anti-interference capability. This study aimed to develop a bacterial biosensor for detecting the total and extractable cadmium in actual environmental samples. We constructed the cadmium-responsive biosensor with the regulatory element (cadmium resistance transcriptional regulatory, CadR) and the reporting element (GFP) and improved its performance by directed evolution. The mutant libraries of biosensors were generated by error-prone PCR and screened by continuous five-round fluorescence-activated cell sorting (FACS), and a bacteria variant epCadR5 with higher performance was finally isolated. Biosensor fluorescence intensity was measured by a microplate reader, and results showed that the evolved cadmium-responsive bacterial biosensor was of high sensitivity and specificity in detecting trace cadmium, with a detection limit of 0.45 μg/L, which is 6.8 times more specific to cadmium than that of the wild-type. Furthermore, microscopic qualitative analysis results showed that the bacteria could produce fluorescence response in a cadmium-contaminated soil matrix, and quantitative analysis results showed that the values of cadmium from epCadR5 bacteria were close to that from inductively coupled plasma-mass spectrometry. These results suggest that the biosensor may have a broad application prospect in the detection of cadmium-contaminated soil and water.
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http://dx.doi.org/10.1021/acs.est.2c00627DOI Listing
July 2022

Characterization of LC-MS based urine metabolomics in healthy children and adults.

PeerJ 2022 22;10:e13545. Epub 2022 Jun 22.

Proteomics Research Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China.

Previous studies reported that sex and age could influence urine metabolomics, which should be considered in biomarker discovery. As a consequence, for the baseline of urine metabolomics characteristics, it becomes critical to avoid confounding effects in clinical cohort studies. In this study, we provided a comprehensive lifespan characterization of urine metabolomics in a cohort of 348 healthy children and 315 adults, aged 1 to 78 years, using liquid chromatography coupled with high resolution mass spectrometry. Our results suggest that sex-dependent urine metabolites are much greater in adults than in children. The pantothenate and CoA biosynthesis and alanine metabolism pathways were enriched in early life. Androgen and estrogen metabolism showed high activity during adolescence and youth stages. Pyrimidine metabolism was enriched in the geriatric stage. Based on the above analysis, metabolomic characteristics of each age stage were provided. This work could help us understand the baseline of urine metabolism characteristics and contribute to further studies of clinical disease biomarker discovery.
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http://dx.doi.org/10.7717/peerj.13545DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233480PMC
June 2022

Camellia ( Abel.) Seed Oil Regulating of Metabolic Phenotype and Alleviates Dyslipidemia in High Fat-Fed Mice through Serum Branch-Chain Amino Acids.

Nutrients 2022 Jun 10;14(12). Epub 2022 Jun 10.

Research Institute of Oil Tea Camellia, Hunan Academy of Forestry, Shao Shan South Road, No. 658, Changsha 410004, China.

Camellia () seed oil (CO) has been shown to effectively reduce the blood lipid level of its host due to its fatty acid content, but the specific molecular mechanism associated with the metabolic phenotype after digestion is not clear. Here, we further investigated the relationship between branched-chain amino acids (BCAA) and the metabolic phenotype that may exhibit the anti-dyslipidemia effect of CO on mice fed a high-fat diet for 30 day C57BL/6J male mice were allocated to three groups: the control group (Cont), the high-fat feed group (HFD), and a high-fat feed group with CO treatment (CO). A serum sample was collected to detect lipid biomarkers and BCAA concentration. Notably, Low-density lipoprotein (LDL), Total Cholesterol (TC), and Triglycerides (TG) showed a significant decrease, whereas High-density lipoprotein (HDL) increased in CO mice but not in the HFD group. The concentration of Isoleucine (Ile), leucine (Leu), and valine (Val) was similar between the Cont and CO groups compared with the HFD group, exhibiting an inhibition induced by CO in mice fed with a high-fat diet. A metabolic phenotype from serum examined by non-targeted metabolite analysis using UHPLC/MS showed most metabolites exhibited lipid and BCAA metabolism. The results indicated that CO treatment notably regulated the metabolism of arachidonic acid and steroid biosynthesis in response to HFD-induced dyslipidemia. In addition, the expression of PPARγ genes that correlated with the BCAA and serum lipid biomarkers were compared, and significant inhibition was noticed, which might lead to the potential exposure of the anti-dyslipidemia mechanism of CO in HFD-fed mice. In conclusion, the expression of PPARγ genes, serum lipid level, BCAA concentration, and the metabolic phenotype was significantly positive in correlation with a high-fat diet, whereas oral CO improved the biomarkers and metabolism of some specific serum metabolites in HFD-fed mice.
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http://dx.doi.org/10.3390/nu14122424DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228151PMC
June 2022

Efficacy of hematopoietic stem cell transplantation in the treatment of children with non-severe aplastic anemia.

Pediatr Transplant 2022 Jun 23:e14340. Epub 2022 Jun 23.

Hematology Center, Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics (Capital Medical University), Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.

Background: Non-severe aplastic anemia is more likely to develop into severe aplastic anemia, and there is no widely accepted treatment plan at present. Hematopoietic stem cell transplantation might be a new therapeutic strategy.

Methods: Retrospectively analyzed 32 patients with non-severe aplastic anemia who underwent hematopoietic stem cell transplantation from September 2007 to September 2020, and the 5-year estimated overall survival rate and the incidence of graft-versus-host disease were analyzed to evaluate the efficacy and safety of hematopoietic stem cell transplantation in the treatment of pediatric non-severe aplastic anemia.

Results: Thirty-two patients who underwent transplantation, 29 patients (90.6%) survived, 3 patients (9.4%) died. The incidence of acute graft-versus-host disease was 51.6% (16/31), including 15 cases (48.4%) of grade I-II and 1 case (3.2%) of grade III-IV. The incidence of chronic graft-versus-host disease was 38.7% (12/31). The 5-year overall survival rate was 91.8%.

Conclusions: Hematopoietic stem cell transplantation is a practicable, safe, and effective treatment option for non-severe aplastic anemia pediatric patients who are suitable for transplant.
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http://dx.doi.org/10.1111/petr.14340DOI Listing
June 2022
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