Publications by authors named "Jie Lu"

1,077 Publications

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Fenofibrate Exerts Antitumor Effects in Colon Cancer via Regulation of DNMT1 and CDKN2A.

PPAR Res 2021 16;2021:6663782. Epub 2021 Apr 16.

Department of Gastroenterology, Shanghai Tenth People's Hospital Affiliated to Tongji University, Tongji University, School of Medicine, Shanghai 200072, China.

Peroxisome proliferator-activated receptor alpha (PPARA) is the molecular target of fibrates commonly used to treat dyslipidemia and diabetes. Recently, the potential role of PPARA in other pathological conditions, such as cancers, has been recognized. Here, using bioinformatics analysis, we found that PPARA was expressed at relatively low levels in pancancers, and Kaplan-Meier analyses revealed that high PPARA protein expression was correlated with better survival of patients with colon cancer. experiments showed that fenofibrate regulated cell cycle distribution, promoted apoptosis, and suppressed cell proliferation and epithelial mesenchymal transition by activating PPARA. PPARA activation inhibited DNMT1 activity and abolished methylation-mediated CDKN2A repression. Downregulation of cyclin-CDK complexes led to the restoration of CDKN2A, which caused cell cycle arrest in the G1 phase via regulation of the CDKN2A/RB/E2F pathway. Finally, we demonstrated that fenofibrate administration inhibited tumor growth and DNMT1 activity . The PPARA agonist, fenofibrate, might serve as an applicable agent for epigenetic therapy of colon cancer patients.
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http://dx.doi.org/10.1155/2021/6663782DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075693PMC
April 2021

OSU-03012 Disrupts Akt Signaling and Prevents Endometrial Carcinoma Progression in vitro and in vivo.

Drug Des Devel Ther 2021 30;15:1797-1810. Epub 2021 Apr 30.

Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, People's Republic of China.

Purpose: OSU-03012 is a celecoxib derivative lacking cyclooxygenase-2 inhibitory activity and a potent PDK1 inhibitor which has been shown to inhibit tumor growth in various ways. However, the role of OSU-03012 in endometrial carcinoma (EC) in which the PI3K/Akt signaling pathway highly activated has not been studied. Here, we determined the potency of OSU-03012 in suppressing EC progression in vitro and in vivo, and studied the underlined mechanisms.

Methods: The human EC Ishikawa and HEC-1A cells were used as the in vitro models. CCK8 assay and flow cytometry were conducted to evaluate cell proliferation, cell cycle progression, and apoptosis. The metastatic ability was evaluated using the transwell migration assay. The Ishikawa xenograft tumor model was used to study the inhibitory effects of OSU-03012 on EC growth in vivo. Western blot analysis was performed to evaluate expressions of the cell cycle and apoptosis associated proteins.

Results: OSU-03012 could inhibit the progression of EC both in vitro and in vivo by disrupting Akt signaling. It reduced the metastatic ability of EC, led to G2/M cell cycle arrest and induced apoptosis via the mitochondrial apoptosis pathway.

Conclusion: Our data indicated that OSU-03012 could inhibit the progression of EC in vitro and in vivo. It can potentially be used as the targeted drug for the treatment of EC by inhibiting Akt signaling.
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http://dx.doi.org/10.2147/DDDT.S304128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096345PMC
April 2021

Stretchable dual cross-linked silicon elastomer with a superhydrophobic surface and fast triple self-healing ability at room temperature.

Soft Matter 2021 May;17(17):4643-4652

State Key Laboratory of Environmental-friendly Energy Materials, Southwest University of Science and Technology, Mianyang 621010, China.

Stretchable elastomers with superhydrophobic surfaces have potential applications in wearable electronics. However, various types of damage inevitably occur on these elastomers in actual application, resulting in the deterioration of the superhydrophobic properties. In this work, superhydrophobic elastomers (HB-imine-BZn-PDMS), was fabricated by employing a dual-layered structure. The bottom layer was a silicon elastomer (imine-BZn-PDMS) with an imine/coordination dual cross-linked structure and room temperature self-healing efficiency of 94%. The top layer was imine-BZn-PDMS/silica nanocomposites to provide superhydrophobic properties. The HB-imine-BZn-PDMS elastomer exhibited fast triple self-healing ability at room temperature toward surface oxidation/decomposition, ruptures, or pinholes, and high durability under abrasion and stretching. The dual dynamic bonds of imine-BZn-PDMS enabled fast recovery of superhydrophobicity in 20 min at room temperature via bond exchange, after generating pinholes across the elastomer. Following surface chemical damage, the HB-imine-BZn-PDMS elastomer also exhibited fast (40 min) room-temperature self-healing ability, which is superior to that of most current self-healing superhydrophobic materials.
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http://dx.doi.org/10.1039/d0sm02175jDOI Listing
May 2021

Association of low-level environmental exposure to cadmium and lead with gout flare using a cohort study design.

Chemosphere 2021 Apr 27;280:130648. Epub 2021 Apr 27.

Institute of Metabolic Diseases, Qingdao University, Qingdao, 266071, China; Shandong Provincial Key Laboratory of Metabolic Diseases and Qingdao Key Laboratory of Gout, the Affiliated Hospital of Qingdao University, China; Medical Research Center, The Affiliated Hospital of Qingdao University, China; Department of Endocrinology and Metabolism, The Affiliated Hospital of Qingdao University, China. Electronic address:

Cadmium (Cd) and lead (Pb) are toxic heavy metals with endocrine-disrupting properties. We investigated the associations of low-level environmental exposure to Cd/Pb and gout status (intercritical gout, gout flare and combined gout) in a cohort study. We measured by ICP-MS the levels of Cd and Pb in blood (Cd-B and Pb-B) and urine (Cd-U and Pb-U) from 408 participants with blood and 346 participants with urine samples recruited from a hospital gout clinic. The median levels of Cd-B and Pb-B (in μg/L) in the gout flare group were 0.87 (range 0.41-2.49) and 31.54 (25.38-41.46), respectively, and the median levels of Cd-U and Pb-U in the gout flare group were 1.05 (0.69-1.91) and 3.86 (3.49-4.44), respectively. These medians were significantly higher than those in the control or intercritical groups (P < 0.05). For Cd-B in tertile 2 (T2) and Cd-U in tertile 3, Cd levels were significantly associated with gout flare status compared to the reference tertile 1 (OR = 4.3, P = 0.041 and OR = 25.1, P = 0.002, respectively) after adjustment under Model 3. For Pb-U, the risk of gout flare status was significantly higher in T2 (OR = 51.0, P = 0.002) compared to the T1 under Model 3. Our results show that median levels of Cd-B, Pb-B, Cd-U and Pb-U in the gout flare group were significantly higher than participants without gout or with gout but in the intercritical period. We provide evidence that the risk of gout flare status is associated with increased Cd levels, and that blood and urine levels of Cd are a risk factor for gout flare status.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130648DOI Listing
April 2021

Neutralizing monoclonal antibodies present new prospects to treat SARS-CoV-2 infections.

Front Med 2021 Apr 28. Epub 2021 Apr 28.

Department of Infectious Diseases, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.

The coronavirus disease 2019 (COVID-19) has caused global public health and economic crises. Thus, new therapeutic strategies and effective vaccines are urgently needed to cope with this severe pandemic. The development of a broadly neutralizing antibody against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is one of the attractive treatment strategies for COVID-19. Currently, the receptor-binding domain (RBD) of the spike (S) protein is the main target of neutralizing antibodies when SARS-CoV-2 enters human cells through an interaction between the S protein and the angiotensin-converting enzyme 2 expressed on various human cells. A single monoclonal antibody (mAb) treatment is prone to selective pressure due to increased possibility of targeted epitope mutation, leading to viral escape. In addition, the antibody-dependent enhancement effect is a potential risk of enhancing the viral infection. These risks can be reduced using multiple mAbs that target nonoverlapping epitopes. Thus, a cocktail therapy combining two or more antibodies that recognize different regions of the viral surface may be the most effective therapeutic strategy.
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http://dx.doi.org/10.1007/s11684-021-0847-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079842PMC
April 2021

Editorial for "Cerebrovascular Reactivity Timing and Stroke Risk in Patients With Intracranial Stenosis".

Authors:
Jie Lu

J Magn Reson Imaging 2021 Apr 20. Epub 2021 Apr 20.

Department of Radiology and Nuclear Medicine, Xuanwu Hospital, Capital Medical University, Beijing, China.

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http://dx.doi.org/10.1002/jmri.27647DOI Listing
April 2021

Sex modifies APOE ε4 dose effect on brain tau deposition in cognitively impaired individuals.

Brain 2021 Apr 20. Epub 2021 Apr 20.

Mallinckrodt Institute of Radiology, Washington University in St. Louis School of Medicine, St. Louis, MO, USA.

Recent studies in cognitively unimpaired elderly individuals suggest that the APOE ε4 allele exerts a dosage-dependent effect on brain tau deposition. The aim of this study was to investigate sex differences in APOE ε4 gene dosage effects on brain tau deposition in cognitively impaired individuals using quantitative 18F-flortaucipir PET. Preprocessed 18F-flortaucipir tau PET images, T1-weighted structural MRI images, demographic information, global cortical amyloid-β burden measured by 18F-florbetapir PET, CSF total tau and phosphorylated tau measurements were obtained from the Alzheimer's Disease Neuroimaging Initiative database. Two hundred and sixty-eight cognitively impaired individuals with 146 APOE ε4 non-carriers and 122 carriers (85 heterozygotes and 37 homozygotes) were included in the study. An iterative reblurred Van Cittert iteration partial volume correction method was applied to all downloaded PET images. MRI images were used for PET spatial normalization. Twelve regional standardized uptake value ratios relative to the cerebellum were computed in standard space. APOE ε4 dosage by sex interaction effect on 18F-flortaucipir standardized uptake value ratios was assessed using generalized linear models and sex-stratified analysis. We observed a significant APOE ε4 dosage by sex interaction effect on tau deposition in the lateral temporal, posterior cingulate, medial temporal, inferior temporal, entorhinal cortex, amygdala, parahippocampal gyrus regions after adjusting for age and education level (P < 0.05). The medial temporal, entorhinal cortex, amygdala and parahippocampal gyrus regions retained a significant APOE ε4 dosage by sex interaction effect on tau deposition after adjusting for global cortical amyloid-β (P < 0.05). In sex-stratified analysis, there was no significant difference in tau deposition between female homozygotes and heterozygotes (P > 0.05). In contrast, male homozygotes standardized uptake value ratios were significantly greater than heterozygotes or non-carriers throughout all twelve regions of interest (P < 0.05). Female heterozygotes exhibited significantly increased tau deposition compared to male heterozygotes in the orbitofrontal, posterior cingulate, lateral temporal, inferior temporal, entorhinal cortex, amygdala and parahippocampal gyrus (P < 0.05). Results from voxelwise analysis were similar to the ones obtained from regions of interest analysis. Our findings suggest that an APOE ε4 dosage effect on brain region-specific tau deposition exists in males, but not females. These results have important clinical implications towards developing sex and genotype-guided therapeutics in Alzheimer's disease and uncovers a potential explanation underlying differential apolipoprotein E ε4-associated Alzheimer's risk in males and females.
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http://dx.doi.org/10.1093/brain/awab160DOI Listing
April 2021

Controllable Microporous Framework Isomerism within Continuous Mesoporous Channels: Hierarchically Porous Structure for Capture of Bulky Molecules.

Inorg Chem 2021 May 19;60(9):6633-6640. Epub 2021 Apr 19.

State Key Laboratory of Materials-Oriented Chemical Engineering, Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Chemical Engineering, Nanjing Tech University, Nanjing 211816, China.

To date, some attempts have been made to synthesize hierarchically porous metal-organic frameworks (HPMOFs), and in most cases, mesopores are formed in microporous frameworks. However, mass transfer and diffusion are still limited in such HPMOFs since micropores connect mesopores and mesopores are noncontinuous. Here, we fabricate a new hierarchical structure through the formation of microporous MOFs within continuous mesoporous channels. Confined space in the as-prepared mesoporous silica-containing template was used to prepare well-dispersed metal precursor of ZnO. The strategy of ligand vapor-induced crystallization was then designed to construct MOFs inside mesoporous channels, in which vapored ligand at elevated temperature diffuses and reacts with metal precursor. Our results indicate that framework isomerism is controllable by adjusting the crystallization conditions. In comparison to their microporous and mesoporous counterparts, the hierarchically porous materials show obviously enhanced adsorption performance on a series of bulky molecules including dye, enzyme, and metal-organic polyhedron.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00438DOI Listing
May 2021

Preparation and biological evaluation of radioiodine-labeled triphenylphosphine derivatives as mitochondrial targeting probes.

J Labelled Comp Radiopharm 2021 Apr 18. Epub 2021 Apr 18.

Key Laboratory of Radiopharmaceuticals, Beijing Normal University, Ministry of Education, Beijing, China.

The positive-charged lipophilic triphenylphosphonium cations (TPPs ) have been served as mitochondrial targeting vehicles for the delivery of various probes. In this study, we developed a new method for the preparation of radioiodine-labeled TPPs . Four I-labeled TPPs , [ I] 9-[ I] 12, were prepared from the corresponding triphenylphosphine phenylborate precursors of B 5-B 8 via an optimized copper-catalyzed one-step procedure in high radiochemical yield (>95%). After radio-HPLC purification, the final products could be obtained with high specific activity. Their physicochemical properties, in vitro cellular uptake, and ex vivo mice biodistribution were investigated. The results suggested the I-labeled TPPs were lipophilic and could specifically accumulate in the mitochondrial-rich myocardial cells through the mitochondrial membrane potential.
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http://dx.doi.org/10.1002/jlcr.3910DOI Listing
April 2021

Expansion and Molecular Characterization of AP2/ERF Gene Family in Wheat ( L.).

Front Genet 2021 31;12:632155. Epub 2021 Mar 31.

College of Agronomy, Anhui Agricultural University, Hefei, China.

The AP2/ERF is a large protein family of transcription factors, playing an important role in signal transduction, plant growth, development, and response to various stresses. AP2/ERF super-family is identified and functionalized in a different plant but no comprehensive and systematic analysis in wheat ( L.) has been reported. However, a genome-wide and functional analysis was performed and identified 322 TaAP2/ERF putative genes from the wheat genome. According to the phylogenetic and structural analysis, TaAP2/ERF genes were divided into 12 subfamilies (Ia, Ib, Ic, IIa, IIb, IIc, IIIa, IIIb, IIIc, IVa, IVb, and IVc). Furthermore, conserved motifs and introns/exons analysis revealed may lead to functional divergence within clades. -Acting analysis indicated that many elements were involved in stress-related and plant development. Chromosomal location showed that 320 AP2/ERF genes were distributed among 21 chromosomes and 2 genes were present in a scaffold. Interspecies microsynteny analysis revealed that maximum orthologous between , rice followed by wheat. Segment duplication events have contributed to the expansion of the AP2/ERF family and made this family larger than rice and . Additionally, AP2/ERF genes were differentially expressed in wheat seedlings under the stress treatments of heat, salt, and drought, and expression profiles were verified by qRT-PCR. Remarkably, the RNA-seq data exposed that AP2/ERF gene family might play a vital role in stress-related. Taken together, our findings provided useful and helpful information to understand the molecular mechanism and evolution of the AP2/ERF gene family in wheat.
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http://dx.doi.org/10.3389/fgene.2021.632155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044323PMC
March 2021

Gender Differences in Hemocyte Immune Parameters of Hong Kong Oyster During Immune Stress.

Front Immunol 2021 31;12:659469. Epub 2021 Mar 31.

Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.

Gender differences in individual immune responses to external stimuli have been elucidated in many invertebrates. However, it is unclear if gender differences do exist in the Hong Kong oyster , one of the most valuable marine species cultivated along the coast of South China. To clarify this, we stimulated post-spawning adult with and lipopolysaccharide (LPS). Gender-based differences in some essential functional parameters of hemocytes were studied flow cytometry. Obvious gender-, subpopulation-, and immune-specific alterations were found in the hemocyte immune parameters of . Three hemocyte subpopulations were identified: granulocytes, semi-granulocytes, and agranulocytes. Granulocytes, the chief phagocytes and major producers of esterase, reactive oxygen species, and nitric oxide, were the main immunocompetent hemocytes. Immune parameter alterations were notable in the accumulation of granulocyte esterase activities, lysosomal masses, nitric oxide levels, and granulocyte numbers in male oysters. These results suggest that post-spawning-phase male oysters possess a more powerful immune response than females. Gender and subpopulation differences in bivalve immune parameters should be considered in the future analysis of immune parameters when studying the impact of pathogenic or environmental factors.
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http://dx.doi.org/10.3389/fimmu.2021.659469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044396PMC
March 2021

Metabolically healthy obesity increases the prevalence of stroke in adults aged 40 years or older: Result from the China National Stroke Screening survey.

Prev Med 2021 Apr 18;148:106551. Epub 2021 Apr 18.

Department of Epidemiology and Health Statistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan Province, China. Electronic address:

Debate over the cardio-cerebrovascular risk associated with metabolically healthy obesity (MHO) continues. In this study we investigated the association of MHO with the risk of stroke among 221,114 individuals aged 40 years or older based on data from the China National Stroke Screening and Prevention Project (CNSSPP), a nationally representative cross-sectional study, during 2014 to 2015. Different metabolic health and obesity phenotypes were defined according to the Adult Treatment Panel III (ATP III) criteria, where obesity was defined as a body mass index (BMI) ≥28 kg/m. Logistic regression models were used to estimate the odds ratios (ORs) and 95% confidence intervals (CIs) for stroke risk associated with different metabolically healthy phenotypes. BMI was used to estimate the mediation effect for metabolic abnormalities to stroke. Compared with the metabolically healthy non-obesity (MHNO) group, individuals with MHO (adjusted OR: 1.21, 95% CI: 1.10,1.33), metabolically unhealthy non-obesity (MUNO) (adjusted OR:1.41, 95% CI: 1.36,1.46), or metabolically unhealthy obesity (MUO) (adjusted OR: 1.70, 95% CI: 1.61,1.80) were found to have an increased risk of stroke. The findings were confirmed robustly by various sensitivity analyses and subgroup analyses. Furthermore, obesity and metabolic abnormalities had an additive interaction for stroke risk with an attributable proportion (AP) of 14.0% in females. BMI played a partial mediating role with the proportion of the effect (PE) at 11.1% in the relationship between metabolic abnormalities and stroke. This study strengthens the evidence that management and interventions in the MHO population may contribute to the primary prevention of stroke.
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http://dx.doi.org/10.1016/j.ypmed.2021.106551DOI Listing
April 2021

Crossed cerebellar diaschisis: risk factors and prognostic value in focal cortical dysplasia by F-FDG PET/CT.

Ann Nucl Med 2021 Apr 11. Epub 2021 Apr 11.

Department of Nuclear Medicine, Xuanwu Hospital, Capital Medical University, No. 45 Changchun Street, Xicheng District, Beijing, 100053, China.

Purpose: Crossed cerebellar diaschisis (CCD) has been widely studied in hemispheric stroke but is less characterized in epilepsy. In this study, we used F-FDG PET/CT to investigate the risk factors for CCD and its prognostic value for intractable epilepsy caused by focal cortical dysplasia (FCD).

Methods: One hundred medically intractable epilepsy patients pathologically diagnosed with FCD postoperatively were included and classified into two groups: CCD+ and CCD-. PET/CT images were analyzed qualitatively by visual assessment and semi-quantitatively using the absolute asymmetry index (|AI|). Clinical factors, including age, sex, body mass index (BMI), age at seizure onset, epilepsy duration, seizure type, seizure frequency, electroencephalography (EEG) and brain magnetic resonance imaging (MRI), were retrospectively assessed from medical records. Follow-up outcomes were evaluated according to the Engel classification at 3, 6, 12, 24 and 36 months postoperatively.

Results: Of the 100 patients, 77 (77.0%) were classified as CCD-, and 23 (23.0%) were classified as CCD + . CCD+ patients had a higher number of lobes involved on PET (3.61 ± 2.16 vs 2.26 ± 1.01, P < 0.001) than CCD- patients. CCD- patients showed more negative MRI results than CCD + patients (P = 0.02). At 12 months postoperatively, 70.7% (29/41) of CCD- patients and 30.8% (4/13) of CCD + patients presented a favourable prognosis (P = 0.02). Significant differences in the average |AI| values in the posterior frontal and anterior temporal lobes were found between CCD+ and CCD- patients (P < 0.05).

Conclusion: The number of lobes involved on PET, structural anomalies on MRI, the |AI| values in the posterior frontal and anterior temporal lobes may be predisposing factors for CCD. CCD occurrence may help predict the prognosis of FCD patients at 12 months postoperatively.
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http://dx.doi.org/10.1007/s12149-021-01613-8DOI Listing
April 2021

Multi-Source Contribution Learning for Domain Adaptation.

IEEE Trans Neural Netw Learn Syst 2021 Apr 9;PP. Epub 2021 Apr 9.

Transfer learning becomes an attractive technology to tackle a task from a target domain by leveraging previously acquired knowledge from a similar domain (source domain). Many existing transfer learning methods focus on learning one discriminator with single-source domain. Sometimes, knowledge from single-source domain might not be enough for predicting the target task. Thus, multiple source domains carrying richer transferable information are considered to complete the target task. Although there are some previous studies dealing with multi-source domain adaptation, these methods commonly combine source predictions by averaging source performances. Different source domains contain different transferable information; they may contribute differently to a target domain compared with each other. Hence, the source contribution should be taken into account when predicting a target task. In this article, we propose a novel multi-source contribution learning method for domain adaptation (MSCLDA). As proposed, the similarities and diversities of domains are learned simultaneously by extracting multi-view features. One view represents common features (similarities) among all domains. Other views represent different characteristics (diversities) in a target domain; each characteristic is expressed by features extracted in a source domain. Then multi-level distribution matching is employed to improve the transferability of latent features, aiming to reduce misclassification of boundary samples by maximizing discrepancy between different classes and minimizing discrepancy between the same classes. Concurrently, when completing a target task by combining source predictions, instead of averaging source predictions or weighting sources using normalized similarities, the original weights learned by normalizing similarities between source and target domains are adjusted using pseudo target labels to increase the disparities of weight values, which is desired to improve the performance of the final target predictor if the predictions of sources exist significant difference. Experiments on real-world visual data sets demonstrate the superiorities of our proposed method.
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http://dx.doi.org/10.1109/TNNLS.2021.3069982DOI Listing
April 2021

A Segment-Based Drift Adaptation Method for Data Streams.

IEEE Trans Neural Netw Learn Syst 2021 Apr 9;PP. Epub 2021 Apr 9.

In concept drift adaptation, we aim to design a blind or an informed strategy to update our best predictor for future data at each time point. However, existing informed drift adaptation methods need to wait for an entire batch of data to detect drift and then update the predictor (if drift is detected), which causes adaptation delay. To overcome the adaptation delay, we propose a sequentially updated statistic, called drift-gradient to quantify the increase of distributional discrepancy when every new instance arrives. Based on drift-gradient, a segment-based drift adaptation (SEGA) method is developed to online update our best predictor. Drift-gradient is defined on a segment in the training set. It can precisely quantify the increase of distributional discrepancy between the old segment and the newest segment when only one new instance is available at each time point. A lower value of drift-gradient on the old segment represents that the distribution of the new instance is closer to the distribution of the old segment. Based on the drift-gradient, SEGA retrains our best predictors with the segments that have the minimum drift-gradient when every new instance arrives. SEGA has been validated by extensive experiments on both synthetic and real-world, classification and regression data streams. The experimental results show that SEGA outperforms competitive blind and informed drift adaptation methods.
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http://dx.doi.org/10.1109/TNNLS.2021.3062062DOI Listing
April 2021

Nigral Iron Deposition Is Associated With Levodopa-Induced Dyskinesia in Parkinson's Disease.

Front Neurosci 2021 22;15:647168. Epub 2021 Mar 22.

Department of Radiology and Nuclear Medicine, Xuanwu Hospital, Capital Medical University, Beijing, China.

Objective: To investigate iron deposition in the substantia nigra (SN) of Parkinson's disease (PD) patients associated with levodopa-induced dyskinesia (LID).

Methods: Seventeen PD patients with LID, 17 PD patients without LID, and 16 healthy controls were recruited for this study. The mean QSM values of the whole, left, and right SN were compared among the three groups. A multivariate logistic regression model was constructed to determine the factors associated with increased risk of LID. The receiver operating characteristic curve of the QSM value of SN in discriminating PD with and without LID was evaluated.

Results: The mean QSM values of the whole and right SN in the PD with LID were higher than those in the PD without LID ( = 0.03, = 0.03). Multivariate logistic regression analysis revealed that the QSM value of whole, left, or right SN was a predictor of the development of LID ( = 0.03, = 0.04, and = 0.04). The predictive accuracy of LID in adding the QSM value of the whole, left, and right SN to LID-related clinical risk factors was 70.6, 64.7, and 67.6%, respectively. The QSM cutoff values between PD with and without LID of the whole, left, and right SN were 148.3, 165.4, and 152.7 ppb, respectively.

Conclusion: This study provides the evidence of higher iron deposition in the SN of PD patients with LID than those without LID, suggesting that the QSM value of the SN may be a potential early diagnostic neuroimaging biomarker for LID.
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http://dx.doi.org/10.3389/fnins.2021.647168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019898PMC
March 2021

Forensic features and genetic legacy of the Baloch population of Pakistan and the Hazara population across Durand line revealed by Y-chromosomal STRs.

Int J Legal Med 2021 Apr 5. Epub 2021 Apr 5.

Department of Human Anatomy, School of Basic Medicine, China Medical University, Shenyang, Liaoning, 110122, People's Republic of China.

The Hazara population across Durand line has experienced extensive interaction with Central Asian and East Asian populations. Hazara individuals have typical Mongolian facial appearances and they called themselves descendants of Genghis Khan's army. The people who speak the Balochi language are called Baloch. Previously, a worldwide analysis of Y-chromosomal haplotype diversity for rapidly mutating (RM) Y-STRs and with PowerPlex Y23 System (Promega Corporation Madison, USA) kit was created with collaborative efforts, but Baloch and Hazara population from Pakistan and Hazara population from Afghanistan were missing. In the current study, Yfiler Plus PCR Amplification Kit loci were examined in 260 unrelated Hazara individuals from Afghanistan, 153 Hazara individuals, and 111 Balochi individuals from Baluchistan Pakistan. For the Hazara population from Afghanistan and Pakistan overall, 380 different haplotypes were observed on these 27 Y-STR loci, gene diversities ranged from 0.51288 (DYS389I) to 0.9257 (DYF387S1), and haplotype diversity was 0.9992. For the Baloch population, every individual was unique at 27 Y-STR loci; gene diversity ranged from 0.5718 (DYS460) to 0.9371(DYF387S1). Twelve haplotypes were shared between 178 individuals, while only two haplotypes among these twelve were shared between 87 individuals in Hazara populations. Rst and Fst pairwise genetic distance analyses, multidimensional scaling plot, neighbor-joining tree, linear discriminatory analysis, and median-joining network were performed, which shed light on the history of Hazara and Baloch populations. The results of our study showed that the Yfiler Plus PCR Amplification Kit marker set provided substantially stronger discriminatory power in the Baloch population of Pakistan and the Hazara population across the Durand line.
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http://dx.doi.org/10.1007/s00414-021-02591-2DOI Listing
April 2021

Immunogenomic Profiling Demonstrate AC003092.1 as an Immune-Related eRNA in Glioblastoma Multiforme.

Front Genet 2021 18;12:633812. Epub 2021 Mar 18.

Department of Neurosurgery/Neuro-oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer, Guangzhou, China.

Enhancer RNAs, a type of long non-coding RNAs (lncRNAs), play a critical role in the occurrence and development of glioma. RNA-seq data from 161 glioblastoma multiforme (GBM) samples were acquired from The Cancer Genome Atlas database. Then, 70 eRNAs were identified as prognosis-related genes, which had significant relations with overall survival (log-rank test, < 0.05). AC003092.1 was demonstrated as an immune-related eRNA by functional enrichment analysis. We divided samples into two groups based on AC003092.1 expression: AC003092.1 High (AC003092.1_H) and AC003092.1 Low (AC003092.1_L) and systematically analyzed the influence of AC003092.1 on the immune microenvironment by single-sample gene-set enrichment analysis and CIBERSORTx. We quantified AC003092.1 and TFPI2 levels in 11 high-grade gliomas, 5 low-grade gliomas, and 7 GBM cell lines. Our study indicates that AC003092.1 is related to glioma-immunosuppressive microenvironment, and these results offer innovative sights into GBM immune therapy.
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http://dx.doi.org/10.3389/fgene.2021.633812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012670PMC
March 2021

Trade-Off and Projecting Effects of Land Use Change on Ecosystem Services under Different Policies Scenarios: A Case Study in Central China.

Int J Environ Res Public Health 2021 03 29;18(7). Epub 2021 Mar 29.

College of Resources and Environmental Sciences, Henan Agricultural University, Zhengzhou 450002, China.

Predicting the spatio-temporal evolution characteristics and trade-off/synergy relationships of ecosystem service value (ESV) under different policy scenarios is of great significance for realizing regional sustainable development. This study established a framework and used the geographical simulation and optimization systems-future land use simulation (GeoSOS-FLUS) model and bivariate local autocorrelation analysis to stimulate and predict the impact of land use change on the ESV of Anyang City from 1995 to 2025. We also explored the trade-offs and synergy among ecosystem services under three policy scenarios (natural evolution, cultivated land protection, and ecological protection) in 2025. Results show that (1) the land use change in Anyang from 1995 to 2025 was significant, and the degree of land use change under the cultivated land and ecological protection scenarios was more moderate than that under the natural evolution scenario; (2) The total ESV decreased between 1995 and 2015, amounting to losses of 1126 million yuan, and the decline from 2015 to 2025 under the natural evolution scenario was more significant than those under the cultivated land protection and ecological protection scenarios; and (3) an obvious synergy was observed between various ecosystem services in Anyang City under different scenarios in 2025, and the most significant synergy was observed under the natural evolution scenario. In terms of spatial distribution, the agglomeration of "high-high" synergy in the west and "low-low" synergy in the central region was significant. Local areas showed "high-low" and "low-high" trade-off relationships scattered between their built land and woodland or cultivated land. The proposed framework can provide certain scientific support for regulating land use and ecosystem services in rapidly urbanized areas.
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http://dx.doi.org/10.3390/ijerph18073552DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036688PMC
March 2021

Engineered Producing Palmitoylethanolamide (PEA) Prevents Colitis in Mice.

Int J Mol Sci 2021 Mar 14;22(6). Epub 2021 Mar 14.

Department of Physiology and Pharmacology "V. Erspamer", Sapienza University of Rome, 00185 Rome, Italy.

Palmitoylethanolamide (PEA) is an -acylethanolamide produced on-demand by the enzyme -acylphosphatidylethanolamine-preferring phospholipase D (NAPE-PLD). Being a key member of the larger family of bioactive autacoid local injury antagonist amides (ALIAmides), PEA significantly improves the clinical and histopathological stigmata in models of ulcerative colitis (UC). Despite its safety profile, high PEA doses are required in vivo to exert its therapeutic activity; therefore, PEA has been tested only in animals or human biopsy samples, to date. To overcome these limitations, we developed an NAPE-PLD-expressing (pNAPE-LP), able to produce PEA under the boost of ultra-low palmitate supply, and investigated its therapeutic potential in a murine model of UC. The coadministration of pNAPE-LP and palmitate led to a time-dependent release of PEA, resulting in a significant amelioration of the clinical and histological damage score, with a significantly reduced neutrophil infiltration, lower expression and release of pro-inflammatory cytokines and oxidative stress markers, and a markedly improved epithelial barrier integrity. We concluded that pNAPE-LP with ultra-low palmitate supply stands as a new method to increase the in situ intestinal delivery of PEA and as a new therapeutic able of controlling intestinal inflammation in inflammatory bowel disease.
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http://dx.doi.org/10.3390/ijms22062945DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999950PMC
March 2021

Exploring the Relationship between Gray and White Matter in Healthy Adults: A Hybrid Research of Cortical Reconstruction and Tractography.

Biomed Res Int 2021 11;2021:6628506. Epub 2021 Mar 11.

Department of Radiology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.

The gray matter (GM) and white matter (WM) are structurally and functionally related in the human brain. Among the numerous neuroimaging studies, yet only a few have investigated these two structures in the same sample. So, there is limited and inconsistent information about how they are correlated in the brain of healthy adults. In this study, we combined cortical reconstruction with diffusion spectrum imaging (DSI) tractography to investigate the relationship between cortical morphology and microstructural properties of major WM tracts in 163 healthy young adults. The results showed that cortical thickness (CTh) was positively correlated with the coherent tract-wise fractional anisotropy (FA) value, and the correlation was stronger in the dorsal areas than in the ventral areas. For other diffusion parameters, CTh was positively correlated with axial diffusivity (AD) of coherent fibers in the frontal areas and negatively correlated with radial diffusivity (RD) of coherent fibers in the dorsal areas. These findings suggest that the correlation between GM and WM is inhomogeneity and could be interpreted with different mechanisms in different brain regions. We hope our research could provide new insights into the studies of diseases in which the GM and WM are both affected.
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http://dx.doi.org/10.1155/2021/6628506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7979294PMC
March 2021

Association of Cycling with Risk of All-Cause and Cardiovascular Disease Mortality: A Systematic Review and Dose-Response Meta-analysis of Prospective Cohort Studies.

Sports Med 2021 Mar 28. Epub 2021 Mar 28.

Department of Epidemiology and Health Statistics, College of Public Health, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, Henan, People's Republic of China.

Background: Cycling has been suggested to be related to risk of all-cause and cardiovascular disease (CVD) mortality. However, a quantitative comprehensive assessment of the dose-response association of cycling with risk of all-cause and CVD mortality has not been reported. We performed a meta-analysis of cohort studies assessing the risk of all-cause and CVD mortality with cycling.

Methods: PubMed and Embase databases were searched for relevant articles published up to December 13, 2019. Random-effects models were used to estimate the summary relative risk (RR) of all-cause and CVD mortality with cycling. Restricted cubic splines were used to evaluate the dose-response association.

Results: We included 9 articles (17 studies) with 478,847 participants and 27,860 cases (22,415 from all-cause mortality and 5445 from CVD mortality) in the meta-analysis. Risk of all-cause mortality was reduced 23% with the highest versus lowest cycling level [RR 0.77, 95% confidence interval (CI) 0.67-0.88], and CVD mortality was reduced 24% (RR 0.76, 95% CI 0.65-0.89). We found a linear association between cycling and all-cause mortality (P = 0.208); the risk was reduced by 9% (RR 0.91, 95% CI 0.86-0.96) with each five metabolic equivalent of task (MET)-h/week increase in cycling. We found an approximately U-shaped association between cycling and CVD mortality (P = 0.034), with the lowest risk at approximately 15 MET-h/week of cycling.

Conclusions: Our findings based on quantitative data suggest that any level of cycling is better than none for all-cause mortality. However, for CVD mortality, one must choose an appropriate level of cycling, with an approximate optimum of 15 MET-h/week (equal to 130 min/week at 6.8 MET).
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http://dx.doi.org/10.1007/s40279-021-01452-7DOI Listing
March 2021

The effect of training intensity on implicit learning rates in schizophrenia.

Sci Rep 2021 Mar 22;11(1):6511. Epub 2021 Mar 22.

Cognition Imaging Schizophrenia Lab, Department of Psychosis Studies, Institute of Psychiatry Psychology and Neuroscience, King's College London, London, UK.

Cognitive impairments in learning and memory are core symptoms of schizophrenia, associated with reduced self-reported quality of life. The most effective treatment of cognitive impairments is drill and practice cognitive training. Still, to date no study has investigated the effect of varying the frequency of training on cognitive outcomes. Here we utilized a verbal memory based language learning task, tapping into implicit cognitive processes, to investigate the role of training intensity on learning rates in individuals with schizophrenia. Data from 47 participants across two studies was utilized, one with a daily training regimen over 5 days and the other with a more intensive schedule of 5 sessions delivered over 2 days. The primary outcome measure was the change in implicit learning performance across five sessions, quantified with the Matthews Correlation Coefficient (MCC). Participants in the daily training group showed improved performance compared to the intensive group only at session 4. This is the first study to show that implicit learning rates are influenced by training intensity, with daily sessions outperforming a more intensive regimen; a period of consolidation overnight may be necessary to optimize cognitive training for individuals with schizophrenia.
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http://dx.doi.org/10.1038/s41598-021-85686-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985318PMC
March 2021

Association of FTO gene methylation with incident type 2 diabetes mellitus: A nested case-control study.

Gene 2021 Jun 20;786:145585. Epub 2021 Mar 20.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan, People's Republic of China. Electronic address:

Objectives: This study aimed to investigate the association of FTO methylation level with type 2 diabetes mellitus (T2DM) in a nested case-control study.

Methods: This nested case-control study included 287 pairs of T2DM cases and controls identified from a rural Chinese cohort study with a 6-year follow-up. Controls were matched to the cases on a 1:1 basis by age, sex, ethnicity, marital status, and residence. Conditional multivariate logistic regression models were used to calculate odds ratios (ORs) and 95% confidence intervals (CIs) for the association of cytosine guanine (CpG) locus and tag-single nucleotide polymorphisms (Tag-SNPs) with T2DM. Spearman correlation analysis was used to evaluate the association between FTO methylation and possible risk factors for T2DM in the control group.

Results: The methylation level on the CpG9 site significantly differs between cases and controls, with a significant association between the CpG9 site methylation and probability of T2DM: OR 2.19 (95%CI: 1.31-3.65) after adjusting for potential confounders. The Tag-SNPs (rs72803657, rs1558902, rs17817449, rs11076023) were not associated with T2DM. Further, FTO methylation was associated with some risk factors for T2DM.

Conclusions: A CpG locus of FTO was positively associated with T2DM, but SNPs were not. FTO methylation were also associated with some T2DM risk factors. Further study with a large sample size and data on metabolic product are needed to confirm the association.
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http://dx.doi.org/10.1016/j.gene.2021.145585DOI Listing
June 2021

Effect of Acupuncture Stimulation of Hegu (LI4) and Taichong (LR3) on the Resting-State Networks in Alzheimer's Disease: Beyond the Default Mode Network.

Neural Plast 2021 8;2021:8876873. Epub 2021 Mar 8.

Department of Radiology, Aerospace Center Hospital, Beijing 100049, China.

It was reported that acupuncture could treat Alzheimer's disease (AD) with the potential mechanisms remaining unclear. The aim of the study is to explore the effect of the combination stimulus of Hegu (LI4) and Taichong (LR3) on the resting-state brain networks in AD, beyond the default network (DMN). Twenty-eight subjects including 14 AD patients and 14 healthy controls (HCs) matched by age, gender, and educational level were recruited in this study. After the baseline resting-state MRI scans, the manual acupuncture stimulation was performed for 3 minutes, and then, another 10 minutes of resting-state fMRI scans was acquired. In addition to the DMN, five other resting-state networks were identified by independent component analysis (ICA), including left frontal parietal network (lFPN), right frontal parietal network (rFPN), visual network (VN), sensorimotor network (SMN), and auditory network (AN). And the impaired connectivity in the lFPN, rFPN, SMN, and VN was found in AD patients compared with those in HCs. After acupuncture, significantly decreased connectivity in the right middle frontal gyrus (MFG) of rFPN ( = 0.007) was identified in AD patients. However, reduced connectivity in the right inferior frontal gyrus (IFG) ( = 0.047) and left superior frontal gyrus (SFG) ( = 0.041) of lFPN and some regions of the SMN (the left inferior parietal lobula ( = 0.004), left postcentral gyrus (PoCG) ( = 0.001), right PoCG ( = 0.032), and right MFG ( = 0.010)) and the right MOG of VN ( = 0.003) was indicated in HCs. In addition, after controlling for the effect of acupuncture on HCs, the functional connectivity of the right cerebellum crus I, left IFG, and left angular gyrus (AG) of lFPN showed to be decreased, while the left MFG of IFPN and the right lingual gyrus of VN increased in AD patients. These findings might have some reference values for the interpretation of the combination stimulus of Hegu (LI4) and Taichong (LR3) in AD patients, which could deepen our understanding of the potential mechanisms of acupuncture on AD.
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http://dx.doi.org/10.1155/2021/8876873DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960059PMC
March 2021

Immunotherapy for recurrent glioblastoma: practical insights and challenging prospects.

Cell Death Dis 2021 Mar 19;12(4):299. Epub 2021 Mar 19.

Departmenlt of Oncology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei Province, China.

Glioblastoma (GB) is the most common high-grade intracranial malignant tumor with highly malignant biological behavior and a high recurrence rate. Although anti-PD-1/PD-L1 antibodies have achieved significant survival benefits in several kinds of solid tumors, the phase III clinical trial Checkmate 143 demonstrated that nivolumab, which targets PD-1, did not achieve survival benefits compared with bevacizumab in recurrent glioblastoma (rGB) patients. Nevertheless, neoadjuvant anti-PD-1 therapy followed by surgery and adjuvant anti-PD-1 therapy could effectively activate local and systemic immune responses and significantly improve the OS of rGB patients. Furthermore, several studies have also confirmed the progress made in applying tumor-specific peptide vaccination or chimeric antigen receptor-T (CAR-T) cell therapy to treat rGB patients, and successes with antibodies targeting other inhibitory checkpoints or costimulatory molecules have also been reported. These successes inspired us to explore candidate combination treatments based on anti-PD-1/PD-L1 antibodies. However, effective predictive biomarkers for clinical efficacy are urgently needed to avoid economic waste and treatment delay. Attempts to prolong the CAR-T cell lifespan and increase T cell infiltration through engineering techniques are addressing the challenge of strengthening T cell function. In this review, we describe the immunosuppressive molecular characteristics of rGB; clinical trials exploring anti-PD-1/PD-L1 therapy, tumor-specific peptide vaccination, and CAR-T cell therapy; candidate combination strategies; and issues related to strengthening T cell function.
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http://dx.doi.org/10.1038/s41419-021-03568-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7979733PMC
March 2021

Impact of fenofibrate therapy on serum uric acid concentrations: a review and meta-analysis.

Endocr J 2021 Mar 16. Epub 2021 Mar 16.

Department of Endocrinology and Metabolism, the Affiliated Hospital of Qingdao University, Qingdao, China.

Fenofibrate is a marketed fibric acid derivative for lipid-lowering in patients with lipid disorders. Numerous studies have proven fenofibrate had a certain effect on serum uric acid, here we conducted this study to quantitatively assess the effect of fenofibrate intervention in modulating serum uric acid concentration and the influence on serum creatinine. The PubMed, Embase and Cochrane were systematically searched for randomized controlled trials update to January, 2020. Primary endpoints focused on serum uric acid concentration and serum creatinine concentration. The pooled effects were calculated as weighted mean difference (WMD) by a random-effects model. Finally, 9 studies representing 487 patients were included in the meta-analysis. The meta-analysis demonstrated that fenofibrate significantly reduced serum uric acid levels (WMD -1.32 mg/dL, 95%CI -1.61 to -1.03, p < 0.001) and an elevated level in serum creatinine (WMD 0.09 mg/dL, 95%CI 0.02 to 0.15, p < 0.001) following fenofibrate therapy compared with placebo. The present study provided strong evidence that fenofibrate intervention exerted a significant reduction on serum uric acid and a mild increase on serum creatinine. Meta-analysis suggested that there were no significant association between the serum uric acid lowering effect with either dose or treatment duration. Overall, our meta-analysis ascertained that fenofibrate have potential therapeutic effects in patients with lipid metabolic abnormalities but with mid nephrotoxicity. There is strong evidence to provide future direction of practical application and clinical researches of fenofibrate.
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http://dx.doi.org/10.1507/endocrj.EJ20-0808DOI Listing
March 2021

Elevated triglyceride-glucose index predicts risk of incident ischaemic stroke: The Rural Chinese Cohort Study.

Diabetes Metab 2021 Mar 12:101246. Epub 2021 Mar 12.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan, People's Republic of China; Department of Biostatistics and Epidemiology, School of Public Health, Shenzhen University Health Science Center, Shenzhen, Guangdong, People's Republic of China. Electronic address:

Aim: As the association between insulin resistance and ischaemic stroke is conflicting, our study aimed to examine the association between triglyceride-glucose (TyG), a surrogate marker of insulin resistance, and incident ischaemic stroke, and also to further assess the potential effect of modification by several known risk factors of stroke.

Methods: The Rural Chinese Cohort Study enrolled 11,777 participants, aged ≥ 40 years, who were free of stroke and cardiovascular disease at baseline during 2007-2008, and who were then followed during 2013-2014. TyG was determined using the following formula: Ln[fasting triglycerides (mg/dL) × fasting glucose (mg/dL)/2]. The relative risk (RR) and 95% confidence interval (CI) of incident ischaemic stroke associated with TyG were estimated using modified Poisson regression models.

Results: During a median follow-up duration of 6 years, 677 new ischaemic stroke cases were identified. After multivariate adjustment, RR (95% CI) values for ischaemic stroke were 1.33 (1.01-1.75), 1.57 (1.17-2.10) and 1.95 (1.34-2.82) in TyG quartile (Q) 2, 3 and 4 groups, respectively, compared with Q1. A significant interaction between TyG index and age for risk of ischaemic stroke (P < 0.001) was also observed. However, no significant interaction was found between TyG index and other potential risk factors of risk for ischaemic stroke, although there were significant positive associations with female, non-smoker, non-drinker, low or moderate physical activity, non-obese and non-type 2 diabetes mellitus groups.

Conclusion: Elevated TyG index is an independent predictor of ischaemic stroke in the general population, and insulin resistance may be positively associated with future stroke risk.
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http://dx.doi.org/10.1016/j.diabet.2021.101246DOI Listing
March 2021

Linc00641 promotes the progression of gastric carcinoma by modulating the miR-429/Notch-1 axis.

Aging (Albany NY) 2021 03 10;13(6):8497-8509. Epub 2021 Mar 10.

Department of Gastrointestinal Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, PR China.

Linc00641 plays different roles in various types of human cancers. However, the function of linc00641 and its underlying mechanism of action in gastric cancer have not been fully elucidated. Therefore, the aim of our current study was to explore the molecular mechanism of linc00641 in gastric cancer. MTT assays, flow cytometry, wound healing assays, and Transwell invasion assays were utilized to measure cell viability, apoptosis, migration and invasion, respectively. Western blotting and RT-PCR assays were carried out to explore the mechanism of linc00641 in gastric cancer cells. We found that silencing linc00641 suppressed the viability and stimulated the apoptosis of gastric cancer cells, while linc00641 overexpression had the opposite effects. Moreover, linc00641 sponged the expression of miR-429 and subsequently upregulated Notch-1 expression in gastric cancer cells. We concluded that linc00641 promoted the malignant progression of gastric cancer by modulating the miR-429/Notch-1 axis.
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http://dx.doi.org/10.18632/aging.202661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034904PMC
March 2021

Lateralizing the affected side of hippocampal sclerosis with quantitative high angular resolution diffusion scalars: a preliminary approach validated by diffusion spectrum imaging.

Ann Transl Med 2021 Feb;9(4):297

Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

Background: Conflicts in regarding the lateralization of the seizure onset for mesial temporal lobe epilepsy (MTLE) are frequently encountered during presurgical evaluation. As a more elaborate, quantified protocol, indices of diffusion spectrum imaging (DSI) might be sensitive to evaluate the seizure involvement. However, the accuracy was less revealed. Herein, we determined the lateralizing value of the DSI indices among MTLE patients.

Methods: Eleven MTLE patients were enrolled together with 11 matched health contrasts. All the participants underwent a DSI scan and with reconstruction of the diffusion scalar, including quantitative anisotropy (QA), isotropic (ISO), and track density imaging (TDI) values. Statistics of these indices were applied to identify the differences between the healthy and ipsilateral sides, and those between the patients and the controls, with special attention to areas of the crura of fornix (FORX), the parahippocampal radiation of the cingulum (PHCR), the hippocampus (HP), parahippocampus (PHC), amygdala (AM) and entorhinal cortex (EC).

Results: Regarding lateralization, TDI of the FORX and the PHCR reached an AUC value of 0.95 and 0.93, respectively (P<0.05), and QA, ISO, TDI of the PHCR, as well as TDI of the FORX were statistically significant amongst the laterals of the patients (P<0.05). Also, the QA of the PHCR were statistically different in the patients' ipsilateral side relative to the contrasts (P<0.017). The diffusion level on different grey matter structures were significantly decreased including HP, AM and EC in GQI space (P<0.017).

Conclusions: The quantitative diffusion scalars of the DSI, especially TDI of the FORX and the PHCR, are sensitive indices to define the ipsilateral side for MTLE patients. For preliminary exploration, the use of quantitative DSI scalars may help to improve the seizure outcome by increasing the accuracy of localization and lateralization for MTLE.
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http://dx.doi.org/10.21037/atm-20-5719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944293PMC
February 2021