Publications by authors named "Jie Liu"

5,366 Publications

  • Page 1 of 1

Alcohol Consumption and Stroke Risk in Men: A Population-Based Cohort Study in Rural Tianjin, China.

Neuroepidemiology 2021 Jun 15:1-9. Epub 2021 Jun 15.

Department of Neurology, Tianjin Medical University General Hospital, Tianjin, China.

Background: Although the protective effects of alcohol consumption against future cardiovascular disease have been published, the effects of alcohol on stroke risk remain controversial.

Method: We assessed the effects of alcohol consumption on stroke risk in a poorly educated, low-income population in rural China. Between 1991 and 2018, a population-based cohort study was conducted in rural Tianjin, China, to examine stroke risk. All registered stroke events were clinically verified using available computed tomography or MRI scans. The stroke risk was analyzed, according to the extent of alcohol consumption, using Cox regression analyses.

Results: We identified 352 incident stroke events among male participants during the study period. The stroke incidences (per 100,000 person-years) were 965.3 overall, 575.9 for ischemic stroke events, 208.4 for hemorrhagic stroke events, and 181.0 for undefined stroke events. Overall, alcohol consumption provided a 32% reduction in the total stroke risk. Low-dose alcohol consumption (≤12 g/day) showed a negative association with total (hazard ratio [HR], 0.64; 95% confidence interval [CI], 0.46-0.88; p = 0.008) and ischemic (HR, 0.66; 95% CI, 0.44-0.98; p = 0.039) strokes. Alcohol consumption was not significantly associated with hemorrhagic strokes. After age stratification, alcohol consumption was protective against total and ischemic strokes in men aged ≥55 years old, with the risk of each stroke type decreasing by 46 and 49%, respectively. Low-dose alcohol consumption was inversely associated with both total and ischemic stroke risks, with the risks decreasing by 56 and 65%, respectively. Alcohol consumption was not significantly associated with strokes among men aged <55 years old.

Conclusions: These findings suggest that low-dose alcohol consumption may decrease the risk of ischemic strokes among men. Even so, the adverse effects of alcohol on the liver and pancreas cannot be ignored. Additionally, the effects of alcohol consumption on stroke risk vary with age, protecting against ischemic and total strokes among males ≥55 years old. Nevertheless, recommending light drinking and its potential health benefits should not be generalized to men of all ages.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1159/000515036DOI Listing
June 2021

Kidney outcomes using a sustained ≥40% decline in eGFR: A meta-analysis of SGLT2 inhibitor trials.

Clin Cardiol 2021 Jun 15. Epub 2021 Jun 15.

Cardiovascular Division, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

Background: A recent meta-analysis of sodium-glucose cotransporter 2 (SGLT2) inhibitor outcome trials reported that SGLT2 inhibitors were associated with reduction in the risk of adverse composite kidney outcomes, with moderate heterogeneity across the trials; however, the endpoints were defined differently across the trials.

Hypothesis: The apparent heterogeneity of the meta-analysis of kidney composite outcomes of SGLT2 inhibitor trials will be substantially reduced by using a consistent assessment of sustained ≥40% decline in eGFR/chronic kidney dialysis/transplantation/renal death across trials.

Methods: We performed a meta-analysis of kidney composite outcomes from the four SGLT2 cardiovascular outcome trial programs conducted in general type 2 diabetes mellitus populations, which included, as a surrogate of progression to kidney failure, a sustained ≥40% decline in eGFR along with kidney replacement therapy and kidney death. The trials assessed were VERTIS CV (NCT01986881), CANVAS Program (NCT01032629 and NCT01989754), DECLARE-TIMI 58 (NCT01730534), and EMPA-REG OUTCOME (NCT01131676).

Results: Data from the trials comprised 42 516 individual participants; overall, 998 composite kidney events occurred. SGLT2 inhibition was associated with a significant reduction in the kidney composite endpoint (HR 0.58 [95% CI 0.51-0.65]) and with a highly consistent effect across the trials (Q statistic p = .64; I  = 0.0%).

Conclusions: Our meta-analysis highlights the value of using similarly defined endpoints across trials and supports the finding of consistent protection against kidney disease progression with SGLT2 inhibitors as a class in patients with type 2 diabetes mellitus who either have established atherosclerotic cardiovascular disease or are at high cardiovascular risk with multiple cardiovascular risk factors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/clc.23665DOI Listing
June 2021

Effect of Perinatal Vitamin D Deficiency on Lung Mesenchymal Stem Cell Differentiation and Injury Repair Potential.

Am J Respir Cell Mol Biol 2021 Jun 14. Epub 2021 Jun 14.

The Lundquist Institute, 117316, Pediatrics, Torrance, California, United States;

Stem cells, including resident lung mesenchymal stem cells (LMSCs), are critically important for injury repair. Compelling evidence links perinatal vitamin D (VD) deficiency to reactive airway disease; however, the effects of perinatal VD deficiency on LMSC function is unknown. We tested the hypothesis that perinatal VD deficiency alters LMSC proliferation, differentiation, and function, leading to an enhanced myogenic phenotype. We also determined whether LMSCs' effects on alveolar type II (ATII) cell function are paracrine-mediated. Using an established rat model of perinatal VD deficiency, we studied the effects of 4 dietary regimens (0, 250, 500, or 1000 IU/kg cholecalciferol-supplemented groups). At postnatal day 21, LMSCs were isolated and, at passage 3, cell proliferation and differentiation (under basal and adipogenic induction conditions) were determined. LMSC paracrine effects on ATII cell proliferation and differentiation were determined by culturing ATII cells in LMSC-conditioned media from different experimental groups. Using flow-cytometry, >95% of cells were CD45-ve, >90% CD90+ve, >58% CD105+ve, and >64% Stro-1+ve, indicating their stem cell phenotype. Compared to the VD-supplemented groups, LMSCs from the VD-deficient group demonstrated suppressed PPARγ, but enhanced Wnt signaling, under basal and adipogenic induction conditions. LMSC from 250 and 500 VD groups effectively blocked the effects of perinatal VD deficiency. LMSC-conditioned media from the VD-deficient group inhibited ATII cell proliferation and differentiation compared to those from the 250 and 500 VD-supplemented groups. These data support the concept that perinatal VD deficiency alters LMSC proliferation and differentiation, potentially contributing to increased respiratory morbidity seen in children born to VD-deficient mothers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1165/rcmb.2020-0183OCDOI Listing
June 2021

Visualizing the Growth and Division of Rat Gut Bacteria by D-Amino Acid-Based Labeling and FISH Staining.

Front Mol Biosci 2021 28;8:681938. Epub 2021 May 28.

Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Rat is a widely used mammalian model for gut microbiota research. However, due to the difficulties of individual culture of many of the gut bacteria, much information about the microbial behaviors in the rat gut remains largely unknown. Here, to characterize the growth and division of rat gut bacteria, we apply a chemical strategy that integrates the use of sequential tagging with D-amino acid-based metabolic probes (STAMP) with fluorescence hybridization (FISH) to rat gut microbiota. Following sequential gavages of two different fluorescent D-amino acid probes to rats, the resulting dually labeled gut bacteria provides chronological information of their cell wall synthesis. After taxonomical labeling with FISH probes, most of which are newly designed in this study, we successfully identify the growth patterns of 15 bacterial species, including two that have not been cultured separately in the laboratory. Furthermore, using our labeling protocol, we record cells growing at different growth stages of a complete cell division cycle, which offers a new scope for understanding basic microbial activities in the gut of mammalian hosts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmolb.2021.681938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193097PMC
May 2021

Multiple Lesions Insertion: boosting diabetic retinopathy screening through Poisson editing.

Biomed Opt Express 2021 May 16;12(5):2773-2789. Epub 2021 Apr 16.

Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.

Deep neural networks have made incredible progress in many computer vision tasks, owing to access to a great amount of data. However, collecting ground truth for large medical image datasets is extremely inconvenient and difficult to implement in practical applications, due to high professional requirements. Synthesizing can generate meaningful supplement samples to enlarge the insufficient medical image dataset. In this study, we propose a new data augmentation method, Multiple Lesions Insertion (MLI), to simulate new diabetic retinopathy (DR) fundus images based on the healthy fundus images that insert real lesions, such as exudates, hemorrhages, microaneurysms templates, into new healthy fundus images with Poisson editing. The synthetic fundus images can be generated according to the clinical rules, i.e., in different DR grading fundus images, the number of exudates, hemorrhages, microaneurysms are different. The generated DR fundus images by our MLI method are realistic with the real texture features and rich details, without black spots, artifacts, and discontinuities. We first demonstrate the feasibility of this method in a DR computer-aided diagnosis (CAD) system, which judges whether the patient has transferred treatment or not. Our results indicate that the MLI method outperforms most of the traditional augmentation methods, i.e, oversampling, under-sampling, cropping, rotation, and adding other real sample methods in the DR screening task.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/BOE.420776DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176793PMC
May 2021

Asian rainbow option pricing formulas of uncertain stock model.

Soft comput 2021 Jun 7:1-25. Epub 2021 Jun 7.

College of Mathematics and Statistics, Huanggang Normal University, Huanggang, 438000 China.

Asian rainbow option is option on the minimum or the maximum of several average prices. In modern financial market, Asian rainbow option is an effective instrument for asset allocation and risk management. The investor with Asian rainbow option enjoys an entitlement to select a max or min from multiple assets with an exercise price at maturity date. The investor has to defray fee to acquire this right, which raises the option pricing issue. This paper mainly explores the pricing of Asian rainbow option in the uncertain financial environment, in which the underlying assets prices are treated as uncertain processes. Here, the pricing formulas of Asian rainbow option are derived under the condition that stock prices obey uncertain differential equations driven by independent Liu processes. Furthermore, some numerical examples are designed to compute the prices of these options.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00500-021-05922-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183340PMC
June 2021

BnA1.CER4 and BnC1.CER4 are redundantly involved in branched primary alcohols in the cuticle wax of Brassica napus.

Theor Appl Genet 2021 Jun 12. Epub 2021 Jun 12.

National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement, Huazhong Agricultural University, Wuhan, 430070, China.

Key Message: The mutations BnA1.CER4 and BnC1.CER4 produce disordered wax crystals types and alter the composition of epidermal wax, causing increased cuticular permeability and sclerotium resistance. The aerial surfaces of land plants are coated with a cuticle, comprised of cutin and wax, which is a hydrophobic barrier for preventing uncontrolled water loss and environmental damage. However, the mechanisms by which cuticle components are formed are still unknown in Brassica napus L. and were therefore assessed here. BnA1.CER4 and BnC1.CER4, encoding fatty acyl-coenzyme A reductases localizing to the endoplasmic reticulum and highly expressed in leaves, were identified and functionally characterized. Expression of BnA1.CER4 and BnC1.CER4 cDNA in yeast (Saccharomyces cerevisiae) induced the accumulation of primary alcohols with chain lengths of 26 carbons. The mutant line Nilla glossy2 exhibited reduced wax crystal types, and wax composition analysis showed that the levels of branched primary alcohols were decreased, whereas those of the other branched components were increased. Further analysis showed that the mutant had reduced water retention but enhanced resistance to Sclerotinia sclerotiorum. Collectively, our study reports that BnA1.CER4 and BnC1.CER4 are fatty acyl-coenzyme A reductase genes in B. napus with a preference for branched substrates that participate in the biosynthesis of anteiso-primary alcohols.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00122-021-03879-yDOI Listing
June 2021

Inverse identification of pollution source release information for surface river chemical spills using a hybrid optimization model.

J Environ Manage 2021 Jun 11;294:113022. Epub 2021 Jun 11.

School of Software, Harbin Institute of Technology, Harbin, 150090, China. Electronic address:

To evaluate the liability of the spilled contaminant and to design comprehensive emergency response schemes, it is essential to estimate the contaminant source characteristic and identify where, when and how much the spilled contaminant is injected into a surface river. In this study, an effective pollution source inverse method is developed to reconstruct the release history of the injection location, time, and quantity, and provide appropriate emergency response schemes for dealing with surface river environmental pollution. The pollution source inverse method IGSAA is developed by an integration of genetic algorithm (IGA) and simulated annealing algorithm (SAA) in order to guarantee both the global searching ability and convergence speed. The pollution source inverse method IGSAA is then applied to a hypothetical study, comparing with the traditional GA-based and SAA-based methods, to verify the accuracy and efficiency of the contaminant source inverse, and to a trace study of Truckee River in west America to identify the contaminant source release history and characteristic under different scenarios. The pollution source inversion results can help decision-makers (DMs) to identify the contaminant source characteristics of a chemical spill, and carry out emergency disposal scheme for an emergency rescue in a quick response, and enhance the supervision and management ability for a real surface river system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jenvman.2021.113022DOI Listing
June 2021

Detection of the level of DNMT1 based on self-assembled probe signal amplification technique in plasma.

Spectrochim Acta A Mol Biomol Spectrosc 2021 May 25;261:120020. Epub 2021 May 25.

College of Public Health, Zhengzhou University, Zhengzhou 450001, China. Electronic address:

DNA (cytosine-5)-methyltransferase1 (DNMT1) is the most abundant DNA methyltransferase in somatic cells, and it plays an important role in the initiation, occurrence, and rehabilitation of tumors. Herein, we developed a novel strategy for the detection of the level of DNMT1 in human plasma using the self-assembled nucleic acid probe signal amplification technology. In this method, the DNMT1 monoclonal antibody (McAb) was immobilized on carboxyl magnetic beads to form immunomagnetic beads and then captured DNMT1 specifically. After that, DNMT1 polyclonal antibody (PcAb) and biotinylated sheep anti-rabbit IgG (sheep anti rabbit IgG-Biotin) were sequentially added into the system to react with DNMT1 and form biotinylated double antibody sandwich immunomagnetic beads. In the presence of the bridging medium streptavidin, the biotinylated double antibody sandwich immunomagnetic beads would form a complex with biotinylated poly-fluorescein (Biotin-poly FAM), and the fluorescence intensity of the complex was proportional to the concentration of DNMT1. Immunomagnetic beads can capture the target DNMT1 in the sample, and Biotin-poly FAM can realize signal amplification. Using these strategies, we got a linear range of the system for DNMT1 level detection was from 2 nmol/L to 200 nmol/L, and the limit of detection (LOD) was 0.05 nmol/L. The method was successfully applied for the determination of DNMT1 in human plasma with the recovery of 101.3-106.0%. Therefore, this method has the potential for the detection of DNMT1 level in clinical diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2021.120020DOI Listing
May 2021

Dapagliflozin: a sodium-glucose cotransporter 2 inhibitor, attenuates angiotensin II-induced cardiac fibrotic remodeling by regulating TGFβ1/Smad signaling.

Cardiovasc Diabetol 2021 06 11;20(1):121. Epub 2021 Jun 11.

Cardiovascular Department, The First Affiliated Hospital, Fujian Medical University, Fujian Institute of Hypertension, 20 Chazhong Road, Fuzhou, 350005, China.

Background: Cardiac remodeling is one of the major risk factors for heart failure. In patients with type 2 diabetes, sodium-glucose cotransporter 2 (SGLT2) inhibitors reduce the risk of the first hospitalization for heart failure, possibly through glucose-independent mechanisms in part, but the underlying mechanisms remain largely unknown. This study aimed to shed light on the efficacy of dapagliflozin in reducing cardiac remodeling and potential mechanisms.

Methods: Sprague-Dawley (SD) rats, induced by chronic infusion of Angiotensin II (Ang II) at a dose of 520 ng/kg per minute for 4 weeks with ALZET® mini-osmotic pumps, were treated with either SGLT2 inhibitor dapagliflozin (DAPA) or vehicle alone. Echocardiography was performed to determine cardiac structure and function. Cardiac fibroblasts (CFs) were treated with Ang II (1 μM) with or without the indicated concentration (0.5, 1, 10 μM) of DAPA. The protein levels of collagen and TGF-β1/Smad signaling were measured along with body weight, and blood biochemical indexes.

Results: DAPA pretreatment resulted in the amelioration of left ventricular dysfunction in Ang II-infused SD rats without affecting blood glucose and blood pressure. Myocardial hypertrophy, fibrosis and increased collagen synthesis caused by Ang II infusion were significantly inhibited by DAPA pretreatment. In vitro, DAPA inhibit the Ang II-induced collagen production of CFs. Immunoblot with heart tissue homogenates from chronic Ang II-infused rats revealed that DAPA inhibited the activation of TGF-β1/Smads signaling.

Conclusion: DAPA ameliorates Ang II-induced cardiac remodeling by regulating the TGF-β1/Smad signaling in a non-glucose-lowering dependent manner.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12933-021-01312-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196449PMC
June 2021

and Serve as Potential Diagnostic Biomarkers of Acute Myocardial Infarction Based on Integrated Bioinformatics Analyses.

DNA Cell Biol 2021 Jun 10. Epub 2021 Jun 10.

Department of Cardiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China.

This study aimed to explore the potential diagnostic biomarkers and mechanisms underlying acute myocardial infarction (AMI). We downloaded four datasets (GSE19339, GSE48060, GSE66360, and GSE97320) from the Gene Expression Omnibus database and combined them as an integrated dataset. A total of 153 differentially expressed genes (DEGs) were analyzed by the linear models for microarray analysis (LIMMA) package. Weighted gene co-expression network analysis was used to screen for the significant gene modules. The intersection of DEGs and genes in the most significant module was termed "common genes" (CGs). CGs were mainly enriched in "inflammatory response," "neutrophil chemotaxis," and "IL-17 signaling pathway" through functional enrichment analyses. Subsequently, 15 genes were identified as the hub genes in the protein-protein interaction network. The Fc fragment of IgE receptor Ig () and prostaglandin-endoperoxide synthase 2 () showed significantly increased expression in AMI patients and mice at the 12-h time point in our experiments. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of and . The area under ROC curve of and was 77.6% and 80.7%, respectively. Moreover, the micro (mi)RNA-messenger (m)RNA network was also visualized; the results showed that miRNA-143, miRNA-144, and miRNA-26 could target in AMI progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/dna.2020.6447DOI Listing
June 2021

Lung Ultrasound Score as a Predictor of Mortality in Patients With COVID-19.

Front Cardiovasc Med 2021 25;8:633539. Epub 2021 May 25.

Department of Ultrasound Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Lung injury is a common condition among hospitalized patients with coronavirus disease 2019 (COVID-19). However, whether lung ultrasound (LUS) score predicts all-cause mortality in patients with COVID-19 is unknown. The aim of the present study was to explore the predictive value of lung ultrasound score for mortality in patients with COVID-19. Patients with COVID-19 who underwent lung ultrasound were prospectively enrolled from three hospitals in Wuhan, China between February 2020 and March 2020. Demographic, clinical, and laboratory data were collected from digital patient records. Lung ultrasound scores were analyzed offline by two observers. Primary outcome was in-hospital mortality. Of the 402 patients, 318 (79.1%) had abnormal lung ultrasound. Compared with survivors ( = 360), non-survivors ( = 42) presented with more B2 lines, pleural line abnormalities, pulmonary consolidation, and pleural effusion (all < 0.05). Moreover, non-survivors had higher global and anterolateral lung ultrasound score than survivors. In the receiver operating characteristic analysis, areas under the curve were 0.936 and 0.913 for global and anterolateral lung ultrasound score, respectively. A cutoff value of 15 for global lung ultrasound score had a sensitivity of 92.9% and specificity of 85.3%, and 9 for anterolateral score had a sensitivity of 88.1% and specificity of 83.3% for prediction of death. Kaplan-Meier analysis showed that both global and anterolateral scores were strong predictors of death (both < 0.001). Multivariate Cox regression analysis showed that global lung ultrasound score was an independent predictor (hazard ratio, 1.08; 95% confidence interval, 1.01-1.16; = 0.03) of death together with age, male sex, C-reactive protein, and creatine kinase-myocardial band. Lung ultrasound score as a semiquantitative tool can be easily measured by bedside lung ultrasound. It is a powerful predictor of in-hospital mortality and may play a crucial role in risk stratification of patients with COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcvm.2021.633539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185027PMC
May 2021

Infection of Two Heterologous Mycoviruses Reduces the Virulence of , a Fungal Agent of Apple Valsa Canker Disease.

Front Microbiol 2021 25;12:659210. Epub 2021 May 25.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Xianyang, China.

Mycovirus infection has been widely shown to attenuate the virulence of phytopathogenic fungi. is an agriculturally important fungus that causes Valsa canker disease in apple trees. In this study, two unrelated mycoviruses [ 1 (CHV1, genus , and single-stranded RNA) and Mycoreovirus 1 (MyRV1, genus , double-stranded RNA)] that originated from (chestnut blight fungus) were singly or doubly introduced into via protoplast fusion. CHV1 and MyRV1 stably infected and caused a reduction in fungal vegetative growth and virulence. Co-infection of both viruses further reduced the virulence of but compromised the stability of CHV1 infection and horizontal transmission through hyphal anastomosis. Infections of MyRV1 and, to a lesser extent, CHV1 up-regulated the transcript expression of RNA silencing-related genes in . The accumulation of CHV1 (but not MyRV1) was elevated by the knockdown of , a key gene of the RNA silencing pathway. Similarly, the accumulation of CHV1 and the efficiency of the horizontal transmission of CHV1 during co-infection was restored by the knockdown of . Thus, CHV1 and MyRV1 are potential biological control agents for apple Valsa canker disease, but co-infection of both viruses has a negative effect on CHV1 infection in due to the activation of antiviral RNA silencing by MyRV1 infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.659210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186502PMC
May 2021

Diffusionless transformation of soft cubic superstructure from amorphous to simple cubic and body-centered cubic phases.

Nat Commun 2021 Jun 9;12(1):3477. Epub 2021 Jun 9.

CAS Key Laboratory of Bio-inspired Materials and Interfaces Sciences, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, China.

In a narrow temperature window in going from the isotropic to highly chiral orders, cholesteric liquid crystals exhibit so-called blue phases, consisting of different morphologies of long, space-filling double twisted cylinders. Those of cubic spatial symmetry have attracted considerable attention in recent years as templates for soft photonic materials. The latter often requires the creation of monodomains of predefined orientation and size, but their engineering is complicated by a lack of comprehensive understanding of how blue phases nucleate and transform into each other at a submicrometer length scale. In this work, we accomplish this by intercepting nucleation processes at intermediate stages with fast cross-linking of a stabilizing polymer matrix. We reveal using transmission electron microscopy, synchrotron small-angle X-ray diffraction, and angle-resolved microspectroscopy that the grid of double-twisted cylinders undergoes highly coordinated, diffusionless transformations. In light of our findings, the implementation of several applications is discussed, such as temperature-switchable QR codes, micro-area lasing, and fabrication of blue phase liquid crystals with large domain sizes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-23631-wDOI Listing
June 2021

Ionic Carbazole-Based Water-Soluble Two-Photon Photoinitiator and the Fabrication of Biocompatible 3D Hydrogel Scaffold.

ACS Appl Mater Interfaces 2021 Jun 9. Epub 2021 Jun 9.

Laboratory of Organic NanoPhotonics and CAS Key Laboratory of Bio-Inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, No. 29 Zhongguancun East Road, Beijing 100190, P. R. China.

Two-photon polymerization of a three-dimensional (3D) hydrogel structure has been widely applied in biological tissue engineering. For improving the biocompatibility of hydrogel structures, a new kind of ionic carbazole water-soluble photoinitiator was prepared to realize the fabrication of a 3D hydrogel structure in aqueous phase. 3,6-Bis[2-(1-methyl-pyridinium)vinyl]-9-methyl-carbazole diiodide (BMVMC) and cucurbit[7]uril (CB7) have been employed to generate a complex with better water solubility by host-guest interactions. The binding ratio of the complex was demonstrated to be 1:1 through the characterization of isothermal titration calorimetry (ITC). The two-photon absorption (TPA) cross section of the complex increases to 2500 GM compared with the 750 GM of the BMVMC molecule. Then, an aqueous-phase photoresist was obtained using the CB7/BMVMC complex as the photoinitiator and poly(ethylene glycol) diacrylate (PEGda) as the hydrogel monomer. Two-photon fabrication capability in aqueous phase has been studied using the as-prepared photoresist. A low laser threshold of 3.7 mW as well as a high resolution of 180 nm are achieved. Benefiting from the fluorescence properties of the photoinitiator, we can achieve the confocal fluorescence images without any assistance of fluorescent probes. Subsequently, a 3D engineered hydrogel scaffold microstructure was fabricated by the two-photon polymerization technology, whose biocompatibility was demonstrated by culturing the structure with living cells of L929. The BMVMC-CB7 complex and the as-prepared photoresist are demonstrated to have good biocompatibility, which is prospective for further application in tissue engineering.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c02227DOI Listing
June 2021

Low-intensity pulsed ultrasound promotes osteoarthritic cartilage regeneration by BMSC-derived exosomes via modulating the NF-κB signaling pathway.

Int Immunopharmacol 2021 Jun 5;97:107824. Epub 2021 Jun 5.

Department of Rehabilitation Medicine, Shunde Hospital of Southern Medical University, Foshan 528000, China; Department of Rehabilitation Medicine, Nanfang Hospital of Southern Medical University, Guangzhou 510000, China. Electronic address:

Osteoarthritis is the most common disabling joint disease throughout the world, and the effect of therapy on its course is still unsatisfactory in clinical practice. Recent studies have shown that mesenchymal stem cell (MSC)-derived exosomes can promote cartilage repair and regeneration in osteoarthritis, indicating that these exosomes could be a novel and promising strategy for treating osteoarthritis. This study investigated whether low-intensity pulsed ultrasound (LIPUS) enhances the effects of bone marrow MSC (BMSC)-derived exosomes on cartilage regeneration in osteoarthritis and examined the underlying mechanism. Our results revealed that BMSC-derived exosomes display the typical morphological features of exosomes. LIPUS-mediated BMSC-derived exosomes promoted cartilage regeneration, increased chondrocyte proliferation and extracellular matrix synthesis, suppressed inflammation, and inhibited the interleukin (IL)-1β-induced activation of the nuclear factor kappa B (NF-κB) pathway. In brief, LIPUS enhances the promoting effects of BMSC-derived exosomes on osteoarthritic cartilage regeneration, mainly by strengthening the inhibition of inflammation and further enhancing chondrocyte proliferation and cartilage matrix synthesis. The underlying mechanism could be related to the inhibition of the IL-1β-induced activation of the NF-κB pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.intimp.2021.107824DOI Listing
June 2021

The role of pulmonary mast cells activation and degranulation in the process of increased pulmonary artery pressure.

Gen Physiol Biophys 2021 May;40(3):183-195

Research Center for High Altitude Medicine, Key Laboratory for High Altitude Medicine, Ministry of Education, Qinghai University, Xining, China.

Hypoxia exposure often cause the increases of pulmonary arterial pressure (PAP). Studies reported that mast cells (MCs) participate in pulmonary vascular remodeling and promote the formation of chronic pulmonary hypertension. Current studies mainly focus on the change of MCs under chronic hypoxia, but few studies on the regulatory role and mechanism of MCs under acute hypoxia. Therefore, present study investigated the dynamic change of MCs in lung tissues under acute hypoxia and the role of MCs activation in the increasement of PAP. In our study, we established an experimental rat model of acute hypobaric hypoxia using a hypobaric chamber (simulated altitude of 7,000 m) and pretreated with MCs degranulation inhibitor sodium cromoglycate (SCG) to study the MCs changes under acute hypoxic exposure. We found that acute hypobaric hypoxia contributed to the increased quantity, activity, and degranulation of MCs and SCG pretreatment showed attenuated PAP elevation under acute hypoxia. Our findings implied that there is a possible mechanism of acute hypoxia cause rapid recruitment of MCs, activation, and explosive degranulation to release Tryptase, Chymase, IL-6, His, 5-HT, and Ang II, which further contributed to pulmonary microvascular contraction and increase in PAP. This work extends the knowledge about MCs, providing a potential profile of MCs as an alternative treatment for high-altitude pulmonary edema (HAPE)-related increased pulmonary artery pressure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4149/gpb_2021007DOI Listing
May 2021

[Design of Pulse Signal Acquisition System of Adaptive Wide Dynamic Range Based on ADS125H02].

Zhongguo Yi Liao Qi Xie Za Zhi 2021 Jun;45(3):284-287

School of Biomedical Engineering, Shenzhen University, Shenzhen, 518060.

In order to solve the problem of wide input range of photocurrent, aiming at the shortage of existing pulse signal acquisition system, an adaptive pulse signal acquisition system based on ADS125H02 is designed, which has high collection accuracy, low noise, anti-interference, wide input range, high integration, etc. The measurement range of the system can reach ±10 V, and the accuracy is 0.009 μV. Experimental tests show that the design of the system can extract weak pulse signals, and has good practicality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3969/j.issn.1671-7104.2021.03.011DOI Listing
June 2021

Development of a high-throughput device for liquid-phase microextraction coupled with gas chromatography-mass spectrometry for the determination of benzene derivatives in environmental water.

Anal Methods 2021 Jun 7. Epub 2021 Jun 7.

College of Chemistry and Materials Science, Northwest University, Xi'an 710127, China.

A device coupled with an automatic gas chromatography injection sampler for high-throughput liquid-liquid microextraction has been proposed in this study. This study's main objective was to devise a trap vial directly placed in an automatic liquid sampler (ALS) to collect and inject the extractant without a transfer step. Sixteen groups of microextraction procedures were performed simultaneously to increase the overall efficiency. The experimental parameters of the extraction method were optimized using a central composite design. Under the optimal conditions, the benzene derivatives' calibration curve was linear in the range of 0.1-200 μg L-1. The limit of detection (LOD) ranged from 0.016 to 0.16 μg L-1, and the limit of quantitation (LOQ) ranged from 0.046 to 0.50 μg L-1. The enrichment factors (EFs) ranged from 254 to 1094. Intraday and interday precision were found to be in the range of 2.2-6.2% (n = 3) and 4.0-6.3% (n = 9), respectively. The relative recovery of the four aqueous samples ranged from 69% to 121%. These experimental results indicated that the proposed device is reliable. Furthermore, it is simple, convenient and high-throughput.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1ay00038aDOI Listing
June 2021

GTPBP2 positively regulates the invasion, migration and proliferation of non-small cell lung cancer.

J Cancer 2021 5;12(13):3819-3826. Epub 2021 May 5.

Department of Pathology, The Fourth Hospital Affiliated to China Medical University, Shenyang, Liaoning. 110032 People's Republic of China.

Lung cancer is one of the most common malignant tumors in the world, and the mortality rate ranks first among various malignant tumors. GTP-binding proteins (guanosine 5'-triphosphate-binding proteins, GTPBPs) are a type of protein with signal transduction function, have GTP hydrolase activity, and play an important role in cell signal transmission, cytoskeletal regulation, protein synthesis and other activities. GTPBP2 is one of the members of the G protein superfamily. Research on GTPBP2 is currently focused on human genetics, and its research in tumors has not been reported. First, Western blot and quantitative real-time PCR were used to analyze the expression differences of 12 cases of GTPBP2 in human NSCLC fresh cancer tissues and adjacent tissues. Then we selected 112 cases of NSCLC cancer tissues and 65 adjacent tissues for immunohistochemistry experiments to analyze the relationships between the expression of GTPBP2 and clinical pathological parameters and prognosis, we found that GTPBP2 is highly expressed in NSCLC cancer tissues, and the high expression of GTPBP2 is related to pTNM stage and lymph node metastasis. In addition, after GTPBP2 knockdown, GTPBP2 can promote the proliferation and invasion of NSCLC cell lines by up-regulating RhoC and MMP-9, and up-regulate cyclinD1, CDK4 and c-myc, and down-regulate P27 to promote the invasion of NSCLC cell lines. In addition, GTPBP2 negatively regulates Axin to promote β-catenin expression, thereby activating Wnt/β-catenin signaling, and promoting the occurrence of NSCLC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/jca.48340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176249PMC
May 2021

Synthesis, SAR study, and bioactivity evaluation of a series of Quinoline-Indole-Schiff base derivatives: Compound 10E as a new Nur77 exporter and autophagic death inducer.

Bioorg Chem 2021 May 23;113:105008. Epub 2021 May 23.

School of Pharmaceutical Sciences and School of Public Health, Xiamen University, Xiamen 361102, China. Electronic address:

We previously reported 5-((8-methoxy-2-methylquinolin-4-yl)amino)-1H-indole- 2-carbohydrazide derivatives as new Nur77 modulators. In this study, we explored whether the 8-methoxy-2-methylquinoline moiety and bicyclic aromatic rings at the N'-methylene position were critical for their antitumor activity against hepatocellular carcinoma (HCC). For this purpose, a small library of 5-substituted 1H-indole-2-carbohydrazide derivatives was designed and synthesized. We found that the 8-methoxy-2-methylquinoline moiety was a fundamental structure for its biological function, while the introduction of the bicyclic aromatic ring into the N'-methylene greatly improved its anti-tumor effect. We found that the representative compound 10E had a high affinity to Nur77. The K values were in the low micromolar (2.25-4.10 μM), which were coincident with its IC values against the tumor cell lines (IC < 3.78 μM). Compound 10E could induce autophagic cell death of liver cancer cells by targeting Nur77 to mitochondria while knocking down Nur77 greatly impaired anti-tumor effect. These findings provide an insight into the structure-activity relation of Quinoline-Indole-Schiff base derivatives and further demonstrate that antitumor agents targeting Nur77 may be considered as a promising strategy for HCC therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bioorg.2021.105008DOI Listing
May 2021

Tuning Surface Energy of Zn Anodes via Sn Heteroatom Doping Enabled by a Codeposition for Ultralong Life Span Dendrite-Free Aqueous Zn-Ion Batteries.

ACS Appl Mater Interfaces 2021 Jun 3. Epub 2021 Jun 3.

State Key Laboratory Base of Eco-chemical Engineering, Taishan Scholar Advantage and Characteristic Discipline Team of Eco-chemical Process and Technology, Qingdao University of Science and Technology, Qingdao 266042, China.

Aqueous Zn-ion batteries (AZBs) have been considered as one of the most promising large-scale energy storage systems, owing to the advantages of raw material abundance, low cost, and eco-friendliness. However, the severe growth of Zn dendrites leads to poor stability and low Coulombic efficiency of AZBs. Herein, to effectively inhibit the growth of Zn dendrites, a new strategy has been proposed, i.e., tuning the surface energy of the Zn anode. This strategy can be achieved by in situ doping of Sn heteroatoms in the lattice of metallic Zn via codeposition of Sn and Zn with a small amount of the SnCl electrolyte additive. Density functional theory calculations have suggested that Sn heteroatom doping can sharply decrease the surface free energy of the Zn anode. As a consequence, driven by the locally strong electric field, metallic Sn tends to deposit at the tips of the Zn anode, thus decreases the surface energy and growth of Zn at the tips, resulting in a dendrite-free Zn anode. The positive effect of the SnCl additive has been demonstrated in both the Zn∥Zn symmetric battery and the Zn/LFP and Zn/HATN full cell. This novel strategy can light a new way to suppress Zn dendrites for long life span Zn-ion batteries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c06002DOI Listing
June 2021

Cobalt-related interstitial lung disease or hard metal lung disease: A case series of Chinese workers.

Toxicol Ind Health 2021 May;37(5):280-288

Department of Occupational Medicine and Toxicology, Clinical Center forInterstitial Lung Diseases, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.

Hard metal lung disease (HMLD) is rarely diagnosed and is caused by the occupational inhalation of hard metal dust, mainly cobalt. The diagnosis of HMLD is based on a thorough occupational dust exposure combined with clinical-radiological-histological findings. We present a series of four Chinese workers who had occupational exposure to cobalt acid lithium or cobalt and tungsten dust. Four patients all complained of intermittent cough, chest tightness, or shortness of breath on exertion. High-resolution computed tomography scans presented bilateral ground-glass attenuation, consolidations, and/or reticular opacities with diffuse small nodules. Histologic findings showed that interstitial inflammation and fibrotic lesions distributed peribronchioles. The infiltrations by macrophages as well as visible multinucleated giant cells indicated giant cell interstitial pneumonia (GIP). Cobalt was detectable in the lung tissues of two patients measured by inductively-coupled plasma mass spectrometry. The first patient was diagnosed with cobalt-related interstitial lung disease (ILD), while the others were HMLD. GIP is the classic pathology of cobalt-related ILD or HMLD. One of the patients showed spontaneous remission after the cessation of exposure, while the other three recovered within 6-32 weeks after avoiding occupational exposure and using corticosteroids. At follow-up, all four patients showed no recurrence. A multidisciplinary diagnostic panel including occupational cobalt exposure evaluation is beneficial to recognize cobalt-related ILD or HMLD and to indicate the necessity of prevention.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/07482337211000989DOI Listing
May 2021

Healable Lithium Alloy Anode with Ultrahigh Capacity.

Nano Lett 2021 Jun 2. Epub 2021 Jun 2.

Soochow Institute for Energy and Materials Innovations, College of Energy, Key Laboratory of Advanced Carbon Materials and Wearable Energy Technologies of Jiangsu Province, Soochow University, Suzhou 215006, People's Republic of China.

Effective recycling of spent Li metal anodes is an urgent need for energy/resource conservation and environmental protection, making Li metal batteries more affordable and sustainable. For the first time, we explore a unique sustainable healable lithium alloy anode inspired by the intrinsic healing ability of liquid metal. This lithium alloy anode can transform back to the liquid state through Li-completed extraction, and then the structure degradation generated during operation could be healed. Therefore, an ultralong cycle life of more than 1300 times can be successfully realized under harsh conditions of 5 mA h cm capacitance by a process of two healing behaviors. This design improves the sustainable utilization of Li metal to a great extent, bringing about unexpected effects in the field of lithium-based anodes even at an unprecedentedly high discharge current density (up to 25 mA cm) and capacity (up to 50 mA h cm).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.nanolett.1c00608DOI Listing
June 2021

Times New Roman',serif;">Characterization of a novel CYP1A2 knockout rat model constructed by CRISPR/Cas9.

Drug Metab Dispos 2021 Jun 1. Epub 2021 Jun 1.

East China Normal University, China

Cytochrome P450 1A2 (CYP1A2) as one of the most important CYP isoforms is involved in the biotransformation of many important endogenous and exogenous substances. CYP1A2 plays an important role in the development of many diseases because it is involved in the biotransformation of precancerous substances and poisons. Although the generation of Cyp1a2 knockout (KO) mouse model has been reported, there are still no relevant rat models for the study of CYP1A2-mediated pharmacokinetics and diseases. In this report, CYP1A2 KO rat model was established successfully by using CRISPR/Cas9 without any detectable off-target effect. Compared with wild-type rats, this model showed a loss of CYP1A2 protein expression in the liver. The results of pharmacokinetics in vivo and incubation in vitro of specific substrates of CYP1A2 confirmed the lack of function of CYP1A2 in KO rats. In further studies of potential compensatory effects, we found that CYP1A1 was significantly up-regulated, and CYP2E1, CYP3A2 and LXRβ were down-regulated in KO rats. In addition, CYP1A2 KO rats exhibited a significant increase in serum cholesterol and free testosterone, accompanied by mild liver damage and lipid deposition, suggesting CYP1A2 deficiency affects lipid metabolism and liver function in rats to some extent. In summary, we successfully constructed the CYP1A2 KO rat model, which provides a useful tool for studying the metabolic function and physiological function of CYP1A2. Human CYP1A2 not only metabolizes clinical drugs and pollutants, but also mediates the biotransformation of endogenous substances, and plays an important role in the development of many diseases. However, there are no relevant CYP1A2 rat models for the research of pharmacokinetics and diseases. This study successfully established CYP1A2 knockout rat model by using CRISPR/Cas9. This rat model provides a powerful tool to study the function of CYP1A2 in drug metabolism and diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1124/dmd.121.000403DOI Listing
June 2021

Magnesium Isoglycyrrhizinate Reduces Hepatic Lipotoxicity through Regulating Metabolic Abnormalities.

Int J Mol Sci 2021 May 30;22(11). Epub 2021 May 30.

Key Lab of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China.

The excessive accumulation of lipids in hepatocytes induces a type of cytotoxicity called hepatic lipotoxicity, which is a fundamental contributor to liver metabolic diseases (such as NAFLD). Magnesium isoglycyrrhizinate (MGIG), a magnesium salt of the stereoisomer of natural glycyrrhizic acid, is widely used as a safe and effective liver protectant. However, the mechanism by which MGIG protects against NAFLD remains unknown. Based on the significant correlation between NAFLD and the reprogramming of liver metabolism, we aimed to explore the beneficial effects of MGIG from a metabolic viewpoint in this paper. We treated HepaRG cells with palmitic acid (PA, a saturated fatty acid of C16:0) to induce lipotoxicity and then evaluated the antagonistic effect of MGIG on lipotoxicity by investigating the cell survival rate, DNA proliferation rate, organelle damage, and endoplasmic reticulum stress (ERS). Metabolomics, lipidomics, and isotope tracing were used to investigate changes in the metabolite profile, lipid profile, and lipid flux in HepaRG cells under different intervention conditions. The results showed that MGIG can indeed protect hepatocytes against PA-induced cytotoxicity and ERS. In response to the metabolic abnormality of lipotoxicity, MGIG curtailed the metabolic activation of lipids induced by PA. The content of total lipids and saturated lipids containing C16:0 chains increased significantly after PA stimulation and then decreased significantly or even returned to normal levels after MGIG intervention. Lipidomic data show that glycerides and glycerophospholipids were the two most affected lipids. For excessive lipid accumulation in hepatocytes, MGIG can downregulate the expression of the metabolic enzymes (GPATs and DAGTs) involved in triglyceride biosynthesis. In conclusion, MGIG has a positive regulatory effect on the metabolic disorders that occur in hepatocytes under lipotoxicity, and the main mechanisms of this effect are in lipid metabolism, including reducing the total lipid content, reducing lipid saturation, inhibiting glyceride and glycerophospholipid metabolism, and downregulating the expression of metabolic enzymes in lipid synthesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms22115884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198484PMC
May 2021

Ferroptosis Suppressive Genes Correlate with Immunosuppression in Glioblastoma.

World Neurosurg 2021 May 29. Epub 2021 May 29.

Department of Neurosurgery, The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Zhujiang Hospital, Southern Medical University, Guangzhou, China. Electronic address:

Background: Glioblastoma (GBM) is the most lethal primary tumor in the central nervous system. Ferroptosis is a type of programmed iron-dependent cell death. In the present study, we aimed to identify prognostic ferroptosis-related genes and their role in tumor immunity.

Methods: We used differential and survival analysis and The Cancer Genome Atlas (TCGA) GBM RNA sequencing data. We also used systematic bioinformatic methods.

Results: Using differential and survival analysis, we found that a ferroptosis suppressor was predominant within ferroptosis-related genes in TCGA GBM RNA sequencing data. By integrating TCGA and gene expression omnibus GBM cohorts, 12 dysregulated ferroptosis suppressors were detected. Among the suppressors, CD44, heat shock protein family B (small) member 1 (HSPB1), and solute carrier family 40 member 1 (SLC40A1) were relevant to overall survival. Using systematic bioinformatic methods, we observed that ferroptosis suppressor expression correlated with immunosuppression, which could be attributed to T-cell exhaustion and cytotoxic T-lymphocyte evasion. Finally, we observed that a potential ferroptosis-inducing drug, acetaminophen, interacted with CD44, HSPB1, and SLC40A1.

Conclusions: The ferroptosis suppressors CD44, HSPB1, and SLC40A1 were significantly associated with prognosis in GBM and correlated with immunosuppression (i.e., T-cell exhaustion and cytotoxic T-lymphocyte evasion). Acetaminophen might have an antitumor function in GBM by regulating CD44, HSPB1, and SLC40A1 to induce ferroptosis. Our results are expected to be of great significance in developing new immunotherapy strategies for GBM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.wneu.2021.05.098DOI Listing
May 2021

miR-506-loaded gelatin nanospheres target PENK and inactivate the ERK/Fos signaling pathway to suppress triple-negative breast cancer aggressiveness.

Mol Carcinog 2021 Jun 1. Epub 2021 Jun 1.

Institute for Special Environmental Biophysics, Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, China.

Triple-negative breast cancer (TNBC) is the most malignant subtype of breast cancer. Some microRNAs (miRNAs) were abnormally expressed in TNBC, and they are closely related to the occurrence and progression of TNBC. Here, we found that miR-506 was significantly downregulated in TNBC and relatively lower miR-506 expression predicted a poorer prognosis. Moreover, we found that miR-506 could inhibit MDA-MB-231 cell viability, colony formation, migration, and invasion, and suppress the ERK/Fos oncogenic signaling pathway through upregulating its direct target protein proenkephalin (PENK). Therefore, miR-506 was proposed as a nucleic acid drug for TNBC therapy. However, miRNA is unstable in vivo, which limiting its application as a therapeutic drug via conventional oral or injected therapies. Here, a gelatin nanosphere (GN) delivery system was applied for the first time to load exogenous miRNA. Exogenous miR-506 mimic was loaded on GNs and injected into the in situ TNBC animal model, and the miR-506 could achieve sustained and controlled release. The results confirmed that overexpression of miR-506 and PENK in vivo through loading on GNs inhibited in situ triple-negative breast tumor growth and metastasis significantly in the xenograft model. Moreover, we indicated that the ERK/Fos signaling pathway was intensively inactivated after overexpression of miR-506 and PENK both in vitro and in vivo, which was further validated by the ERK1/2-specific inhibitor SCH772984. In conclusion, this study demonstrates that miR-506-loaded GNs have great potential in anti-TNBC aggressiveness therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/mc.23310DOI Listing
June 2021

Dissecting the genome of star fruit (Averrhoa carambola L.).

Hortic Res 2020 Jun 1;7(1):94. Epub 2020 Jun 1.

State Key Laboratory of Agricultural Genomics, China National GeneBank, BGI-Shenzhen, 518120, Shenzhen, China.

Averrhoa carambola is commonly known as star fruit because of its peculiar shape, and its fruit is a rich source of minerals and vitamins. It is also used in traditional medicines in countries such as India, China, the Philippines, and Brazil for treating various ailments, including fever, diarrhea, vomiting, and skin disease. Here, we present the first draft genome of the Oxalidaceae family, with an assembled genome size of 470.51 Mb. In total, 24,726 protein-coding genes were identified, and 16,490 genes were annotated using various well-known databases. The phylogenomic analysis confirmed the evolutionary position of the Oxalidaceae family. Based on the gene functional annotations, we also identified enzymes that may be involved in important nutritional pathways in the star fruit genome. Overall, the data from this first sequenced genome in the Oxalidaceae family provide an essential resource for nutritional, medicinal, and cultivational studies of the economically important star-fruit plant.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41438-020-0306-4DOI Listing
June 2020

Laparoscopic vs. Open Repair Surgery for the Treatment of Communicating Hydrocele in Children: A Retrospective Study From a Single Center.

Front Surg 2021 12;8:671301. Epub 2021 May 12.

Department of Pediatric Surgery, Yijishan Hospital of Wannan Medical College, Wannan Medical College, Wuhu, China.

This study evaluated the outcomes of laparoscopic repair (LR) and open repair (OR) surgery for communicating hydrocele in children. We collected the clinical data and follow-up data of all boys (<14 years) who underwent communicating hydrocele surgery in the pediatric surgery department at Yijishan Hospital of Wannan Medical College from January 2017 to December 2018 and retrospectively analyzed the data. In this study, 155 patients were retrospectively enrolled, including 90 patients in the OR group and 65 patients in the LR group. There were significant differences in operation time and the recurrence of hydrocele between the two groups. The persistence of scrotal swelling in the LR group was significantly lower than that in the OR group. There was no significant difference in postoperative hospitalization time or incision infection rate between the two groups. In conclusion, this study shows that laparoscopic treatment of children with communicating hydrocele has the advantages of a hidden incision, a shortened operation time, and a reduced postoperative recurrence rate and can be used as the preferred surgical method. However, laparoscopic treatment should be selected according to the specific condition of each child and cannot completely replace traditional open surgery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fsurg.2021.671301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149793PMC
May 2021