Publications by authors named "Jie Liu"

6,233 Publications

  • Page 1 of 1

Pocahemiketone A, a Sesquiterpenoid Possessing a Spirocyclic Skeleton with a Hemiketal Endoperoxide Unit, Alleviates Aβ-Induced Pyroptosis and Oxidative Stress in SH-SY5Y Cells.

Org Lett 2022 Jun 24. Epub 2022 Jun 24.

Pocahemiketone A, a novel sesquiterpenoid possessing a unique spirocyclic skeleton with a hemiketal endoperoxide unit, was isolated from the essential oil of . Its structure was determined by spectroscopic methods and single-crystal X-ray diffraction analyses. Pocahemiketone A exhibits a significant neuroprotective effect against Aβ-induced damage in SH-SY5Y cells by inhibiting NLRP3 inflammasome-mediated pyroptosis and oxidative stress. These results indicate that pocahemiketone A has great potential for use in the treatment of Alzheimer's disease.
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http://dx.doi.org/10.1021/acs.orglett.2c01587DOI Listing
June 2022

A temperature-responsive selenium nanohydrogel for strawberry grey mould management.

J Mater Chem B 2022 Jun 24. Epub 2022 Jun 24.

Department of Chemistry, College of Chemistry and Materials Science, Jinan University, Guangzhou, 511436, China.

Grey mould is a fungal disease caused by (), which can cause serious damage to a variety of crops. Herein, we developed iprodione (Ipr) reagent-loaded mesoporous selenium nanoparticles (MSe NPs), combined them with low-melting agarose (LA), and obtained a temperature-responsive selenium particle nanogel ([email protected]@LA NPs) using a simple method. Importantly, [email protected]@LA could capture and quickly be softened to realize the controlled release of Ipr, and effectively inhibit and kill . Plate-based antibacterial tests showed that the colony area of the [email protected]@LA NPs was 4.27 cm, which was much smaller than that of the control (25 cm). In addition, the [email protected]@LA NPs showed good biocompatibility, and they could improve the photosynthetic efficiency of plants and promote plant growth. Measurement of the fluorescence parameters showed that the maximum photochemical efficiency (/) of the plant leaves of the inoculated group () is 0.58, but the / value of the [email protected]@LA group is higher than 0.8. In particular, [email protected]@LA NPs could prolong the storage time of strawberries, thereby preserving their freshness. Overall, [email protected]@LA NPs exhibit excellent effects in terms of controlling strawberry gray mould and prolonging the fruit storage time, and this is expected to become a promising strategy for developing intelligent pesticide formulations.
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http://dx.doi.org/10.1039/d2tb00345gDOI Listing
June 2022

A Video Article: The Laparoscopic Uterine Artery Occlusion in Combination with Myomectomy for Uterine Myoma.

Gynecol Minim Invasive Ther 2022 Apr-Jun;11(2):114-115. Epub 2022 May 4.

Department of Gynecology and Obstetrics, Tenth People's Hospital Affiliated to Tongji University, Shanghai, China.

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http://dx.doi.org/10.4103/GMIT.GMIT_10_21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9212172PMC
May 2022

Immunogenicity of Varicella Zoster Virus DNA Vaccines Encoding Glycoprotein E and Immediate Early Protein 63 in Mice.

Viruses 2022 Jun 2;14(6). Epub 2022 Jun 2.

National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China.

Herpes zoster (HZ) is caused by the reactivation of latent varicella-zoster virus (VZV) from the sensory ganglia due to aging or immunosuppression. Glycoprotein E (gE) is a widely used vaccine antigen for specific humoral and cellular immune responses. Immediate early protein 63 (IE63) is expressed during latency, suggesting that it is a potential antigen against HZ reactivation. In this study, HZ DNA vaccines encoding gE, IE63, IE63-2A-gE (where 2A is a self-cleaving sequence), or IE63-linker-gE were developed and investigated for immunogenicity in mice. The results showed that each HZ DNA vaccine induced VZV-specific antibody production. The neutralizing antibody titer elicited by IE63-2A-gE was comparable to that elicited by gE or live attenuated HZ vaccine (LAV). IE63-2A-gE-induced gE or IE63-specific INF-γ T cell frequencies in splenocytes were comparable to those of LAV. Furthermore, IE63-2A-gE, gE, or IE63 led to a significant increase in IFN-γ (IE63 stimulation) and IL-2 (gE stimulation) secretion compared to LAV, showing a Th1-biased immune response. Moreover, IE63-2A-gE and gE induced cytotoxic activity of CD8 T cells compared to that of LAV. This study elucidates that the IE63-2A-gE DNA vaccine can induce both humoral and cell-mediated immune responses, which provides a candidate for the development of an HZ vaccine.
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http://dx.doi.org/10.3390/v14061214DOI Listing
June 2022

Properties of Basalt Fiber Core Rods and Their Application in Composite Cross Arms of a Power Distribution Network.

Polymers (Basel) 2022 Jun 16;14(12). Epub 2022 Jun 16.

Key Laboratory of Physical and Chemical Analysis for Electric Power of Hainan Province, Hairuihou Street No. 23, Haikou 570100, China.

As basalt fiber has better mechanical properties and stability than glass fiber, cross arms made of continuous basalt-fiber-reinforced epoxy matrix composites are capable of meeting the mechanical requirements in the event of typhoons and broken lines in coastal areas, mountainous areas and other special areas. In this paper, continuous basalt-fiber-reinforced epoxy matrix composites were used to fabricate the core rods and composite cross arms. The results verified that basalt fiber composite cross arms can meet the strict requirements of transmission lines in terms of quality and reliability. In addition to high electrical insulation performance, the flexural modulus and the flexural strength of basalt fiber core rods are 1.8 and 1.06 times those of glass fiber core rods, respectively. Basalt fiber core rods were found to be much better load-bearing components compared to glass fiber core rods. However, the leakage current and the result of scanning electron microscopy (SEM) analysis reveal that the interface bonding strength between basalt fibers and the matrix resin is weak. A 3D reconstruction of micro-CT indicates that the volume of pores inside basalt fiber core rods accounts for 0.0048% of the total volume, which is greater than the 0.0042% of glass fiber rods. Therefore, improving the interface bond between basalt fibers and the resin can further improve the properties of basalt fiber core rods.
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http://dx.doi.org/10.3390/polym14122443DOI Listing
June 2022

Blending Modification of Alicyclic Resin and Bisphenol A Epoxy Resin to Enhance Salt Aging Resistance for Composite Core Rods.

Polymers (Basel) 2022 Jun 13;14(12). Epub 2022 Jun 13.

Hebei Provincial Key Laboratory of Power Transmission Equipment Security Defence, North China Electric Power University, Yonghua North Street No. 619, Baoding 071003, China.

In order to promote the application of composite insulators in coastal areas with high temperature, high humidity and high salt, it is of great importance to develop matrix resin with salt corrosion resistance for composite core rods. In this study, bisphenol A epoxy resin was modified by blending with alicyclic epoxy resin (2021P). Three different proportions of 2021P/DGEBA blend resins (0% 2021P/DGEBA, 10% 2021P/DGEBA and 20% 2021P/DGEBA) were prepared, and the high salt medium corrosion test was carried out. The physicochemical (FTIR, DMA, TGA) and electrical properties (dielectric loss, leakage current and breakdown field strength) of the blend resin before and after aging were tested and analyzed, and the optimal blend proportion was determined. The results showed that after salt aging, the Tg of 0% 2021P/DGEBA decreased to 122.99 °C, while the Tg of 10% 2021P/DGEBA reached 134.89 °C; The leakage current of 0% 2021P/DGEBA increased to 48.994 μA, while that of 10% 2021P/DGEBA only increased to 44.549 μA; The breakdown field strength of 0% 2021P/DGEBA dropped to 40.36 kv/mm, while that of 10% 2021P/DGEBA only dropped to 43.63 kv/mm. The introduction of 2021P enhanced the salt corrosion resistance of the blend resin, which could hinder the penetration, diffusion and erosion of external media (such as Na, Cl, HO, etc.) to the matrix resin. The comprehensive properties of 10% 2021P/DGEBA blend system reached the best, which was better than other blending resins, showing great application potential.
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http://dx.doi.org/10.3390/polym14122394DOI Listing
June 2022

New insights into humic acid-boosted conversion of bisphenol A by laccase-activated co-polyreaction: Kinetics, products, and phytotoxicity.

J Hazard Mater 2022 Aug 3;436:129269. Epub 2022 Jun 3.

College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.

How humic acid (HA) modifies bisphenol A (BPA) conversion in exoenzyme-activated polyreaction is poorly understood. Herein, the influencing mechanism of HA on laccase-induced BPA self-polymerization was investigated, and the phytotoxicity of the produced BPA self/co-polymers was assessed for the first time. HA prominently boosted BPA elimination, and the rate constants of BPA conversion augmented from 0.61 to 1.43 h as HA level raised from 0 to 50 mg·L. It is because the generated BPA-HA co-polymers promptly lowered the yields of long-chain BPA self-oligomers, consequently maintaining laccase activity through opening enzymatic substrate-binding pockets. Notably, a few BPA monomers were re-released from the loosely bound self-polymers and co-polymers, and the releasing amounts respectively were 13.9 - 22.4% and 0.3 - 0.5% at pH 2 - 11. Formation of self/co-polymers was greatly conducive to avoiding BPA biotoxicity. Compared with BPA self-polymers, the phytotoxicity of BPA co-polymers to germinated radish (Raphanus sativus L.) seeds was lower due to these covalently bound products were more complex and stable. It follows that laccase-mediated co-polymerization played a significant role in BPA conversion, contaminant detoxification, and carbon sequestration. These findings are not only beneficial to clarifying exoenzyme-activated the generation mechanism of BPA co-polymers in water, but to reusing these supramolecular aggregates in crop growth.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129269DOI Listing
August 2022

Surgesture: a novel instrument based on surgical actions for objective skill assessment.

Surg Endosc 2022 Jun 23. Epub 2022 Jun 23.

Department of Pancreatic Surgery, West China Hospital of Sichuan University, Chengdu, China.

Background: Due to varied surgical skills and the lack of an efficient rating system, we developed Surgesture based on elementary functional surgical gestures performed by surgeons, which could serve as objective metrics to evaluate surgical performance in laparoscopic cholecystectomy (LC).

Methods: We defined 14 LC basic Surgestures. Four surgeons annotated Surgestures among LC videos performed by experts and novices. The counts, durations, average action time, and dissection/exposure ratio (D/E ratio) of LC Surgestures were compared. The phase of mobilizing hepatocystic triangle (MHT) was extracted for skill assessment by three professors using a modified Global Operative Assessment of Laparoscopic Skills (mGOALS).

Results: The novice operation time was significantly longer than the expert operation time (58.12 ± 19.23 min vs. 26.66 ± 8.00 min, P < 0.001), particularly during MHT phase. Novices had significantly more Surgestures than experts in both hands (P < 0.05). The left hand and inefficient Surgesture of novices were dramatically more than those of experts (P < 0.05). The experts demonstrated a significantly higher D/E ratio of duration than novices (0.79 ± 0.37 vs. 2.84 ± 1.98, P < 0.001). The counts and time pattern map of LC Surgestures during MHT demonstrated that novices tended to complete LC with more types of Surgestures and spent more time exposing the surgical scene. The performance metrics of LC Surgesture had significant but weak associations with each aspect of mGOALS.

Conclusion: The newly constructed Surgestures could serve as accessible and quantifiable metrics for demonstrating the operative pattern and distinguishing surgeons with various skills. The association between Surgestures and Global Rating Scale laid the foundation for establishing a bridge to automated objective surgical skill evaluation.
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http://dx.doi.org/10.1007/s00464-022-09108-xDOI Listing
June 2022

miR-143-Mediated Responses to Betaine Supplement Repress Lipogenesis and Hepatic Gluconeogenesis by Targeting MAT1a and MAPK11.

J Agric Food Chem 2022 Jun 23. Epub 2022 Jun 23.

Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou, 510642 China.

The liver as the central organ is responsible for lipogenesis, gluconeogenesis and one-carbon metabolism. Methyl donors (e.g., betaine) modulate metabolic homeostasis and gene regulation through one-carbon metabolism. MiR-143 regulates DNA methylation by targeting DNMT3A, thereby suggesting that this miRNA participates in one-carbon metabolic pathways. However, the effect and mechanism that regulate glucose and lipid metabolism via the methyl group metabolism pathway remain elusive. In this study, we found that a betaine supplement and miR-143 KO significantly promoted lipolysis and glucose utilization and repressed lipogenesis and gluconeogenesis through enhancing energy consumption and thermogenesis, repressing GPNMB and targeting MAPK11, respectively. We further explored the relationship between miR-143 and a methyl donor (betaine) and the miR-143-mediated responses to the betaine supplement regulating the mechanism of the glucose and lipid metabolism. The results showed that betaine significantly down-regulated the expression of miR-143 that subsequently increased SAM levels in the liver by targeting MAT1a. In brief, the regulations of glucose and lipid metabolism are related to the miR-143-regulation of one-carbon units, and the relationship between betaine and miR-143 in the methionine cycle is a typical yin-yang type of regulation. Thus, betaine and miR-143 function together as key regulators and biomarkers for preventing and diagnosing metabolic diseases such as fatty liver disease, obesity, and diabetes.
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http://dx.doi.org/10.1021/acs.jafc.2c02940DOI Listing
June 2022

Gene Expression Microarray Data Identify Hub Genes Involved in Osteoarthritis.

Front Genet 2022 6;13:870590. Epub 2022 Jun 6.

Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, China.

The present study was performed to explore the underlying molecular mechanisms and screen hub genes of osteoarthritis (OA) bioinformatics analysis. In total, twenty-five OA synovial tissue samples and 25 normal synovial tissue samples were derived from three datasets, namely, GSE55457, GSE55235, and GSE1919, and were used to identify the differentially expressed genes (DEGs) of OA by R language. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of DEGs were conducted using the Database for Annotation, Visualization, and Integrated Discovery (DAVID). A Venn diagram was built to show the potential hub genes identified in all three datasets. The STRING database was used for constructing the protein-protein interaction (PPI) networks and submodules of DEGs. We identified 507 upregulated and 620 downregulated genes. Upregulated DEGs were significantly involved in immune response, MHC class II receptor activity, and presented in the extracellular region, while downregulated DEGs were mainly enriched in response to organic substances, extracellular region parts, and cadmium ion binding. Results of KEGG analysis indicated that the upregulated DEGs mainly existed in cell adhesion molecules (CAMs), while downregulated DEGs were significantly involved in the MAPK signaling pathway. A total of eighteen intersection genes were identified across the three datasets. These include Nell-1, ATF3, RhoB, STC1, and VEGFA. In addition, 10 hub genes including CXCL12, CXCL8, CCL20, and CCL4 were found in the PPI network and module construction. Identification of DEGs and hub genes associated with OA may be helpful for revealing the molecular mechanisms of OA and further promotes the development of relevant biomarkers and drug targets.
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http://dx.doi.org/10.3389/fgene.2022.870590DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207392PMC
June 2022

Sex-Related Differences in the Incidence and Development of Carotid Plaques in a Low-Income Chinese Population-A Prospective Cohort Study.

Int J Womens Health 2022 16;14:787-795. Epub 2022 Jun 16.

Center of Clinical Epidemiology & Evidence-Based Medicine, Tianjin Jizhou People's Hospital, Tianjin, 301900, People's Republic of China.

Purpose: More than 150 million people are estimated to have been examined for the presence of carotid plaques (CPs) in China; a sex-related imbalance in the prevalence exists. However, the relationship between sex and the incidence of CP development is unclear, especially in low-income areas of China. Hence, this study aimed to identify the sex differences in CP development and CP burden in both sexes in this population.

Methods: The study population included individuals aged ≥45 years in a rural area of Tianjin, China. Carotid ultrasonography was performed in the 2014 and 2019 cohorts, and information on carotid ultrasonography, including the formation and number of CPs, was collected twice. Logistic analyses were used to investigate the predictors of CP formation and numbers of plaques.

Results: A total of 1479 participants were analyzed. The incidence of CP was 20.3% and 29.0% in women and men, respectively. In women, high low-density lipoprotein cholesterol (LDL-C) levels was independent predictors of CP formation (RR: 1.217, 95%CI: 1.010, 1.461; P=0.039). For men, the corresponding predictors were hypertension, alcohol consumption, and low high-density lipoprotein cholesterol (HDL-C) levels (all P<0.05); none of the examined factors were associated with plaque numbers.

Conclusion: In the study population, men had a higher incidence of plaque than women. Predictors of CP are different in men and women. LDL-C control is critical for moderating atherosclerosis in women; in men, managing blood pressure, stopping alcohol consumption, and controlling HDL-C levels are important.
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http://dx.doi.org/10.2147/IJWH.S365242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208630PMC
June 2022

Adding 5-day decitabine to the conditioning regimen for haploidentical bone marrow transplantation in aplastic anaemia patients results in satisfactory clinical outcomes.

Bone Marrow Transplant 2022 Jun 22. Epub 2022 Jun 22.

Department of Hematology, First Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, PR China.

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http://dx.doi.org/10.1038/s41409-022-01729-zDOI Listing
June 2022

Plasma apelin and vascular endothelial growth factor levels in preterm infants: relationship to neonatal respiratory distress syndrome.

J Matern Fetal Neonatal Med 2022 Jun 22:1-8. Epub 2022 Jun 22.

Department of Pediatrics, Peking University People's Hospital, Beijing, China.

Aim: The study aimed to determine the association between cord plasma levels of apelin and vascular endothelial growth factor (VEGF) with respiratory distress syndrome (RDS) in preterm infants.

Methods: This case-control study included 120 preterm infants admitted to the neonatal intensive care unit of our hospital between January 2019 and January 2020. The infants were divided into RDS ( = 60) and non-RDS groups ( = 60). The cord plasma apelin and VEGF levels, perinatal characteristics, and neonatal complications were compared between the two groups.

Results: The plasma apelin levels in the RDS group were significantly higher than in the non-RDS group (158.9 ± 24.8 vs. 125.2 ± 18.2 pg/mL, respectively), whereas VEGF levels in the non-RDS group were significantly higher than in the RDS group (187.4 ± 28.5 vs. 245.1 ± 44.8 pg/mL, respectively) (both  < .001). Infants with more severe RDS had higher plasma apelin levels and lower plasma VEGF levels. In the receiver operating characteristic curve analysis for the prediction of RDS, a cutoff of 148.4 pg/mL for apelin level yielded a sensitivity of 63.3% and a specificity of 95.0%, whereas a cutoff of 214.2 pg/mL for VEGF level showed a sensitivity of 86.7% and a specificity of 75.0%. Apelin levels were negatively correlated with VEGF levels in infants with RDS ( = 0.84,  < .001).

Conclusion: Differences in cord plasma apelin and VEGF levels may aid in the early diagnosis and treatment of RDS in preterm infants.
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http://dx.doi.org/10.1080/14767058.2022.2089554DOI Listing
June 2022

Privacy protection generalization with adversarial fusion.

Math Biosci Eng 2022 May;19(7):7314-7336

Beijing Friendship Hospital, Beijing 100050, China.

Several biometric privacy-enhancing techniques have been appraised to protect face image privacy. However, a face privacy protection algorithm is usually designed for a specific face recognition algorithm. When the structure or threshold of the face recognition algorithm is fine-tuned, the protection algorithm may be invalid. It will cause the network bloated and make the image distortion target multiple FRAs through the existing technology simultaneously. To address this problem, a fusion technology is developed to cope with the changeable face recognition algorithms via an image perturbation method. The image perturbation is performed by using a GAN-improved algorithm including generator, nozzles and validator, referred to as the Adversarial Fusion algorithm. A nozzle structure is proposed to replace the discriminator. Paralleling multiple face recognition algorithms on the nozzle can improve the compatibility of the generated image. Next, a validator is added to the training network, which takes part in the inverse back coupling of the generator. This component can make the generated graphics have no impact on human vision. Furthermore, the group hunting theory is quoted to make the network stable and up to 4.8 times faster than other models in training. The experimental results show that the Adversarial Fusion algorithm can not only change the image feature distribution by over 42% but also deal with at least 5 commercial face recognition algorithms at the same time.
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http://dx.doi.org/10.3934/mbe.2022345DOI Listing
May 2022

Design, synthesis, antibacterial activity evaluation and molecular docking study of pleuromutilin derivatives bearing amide side chains.

Chem Biol Drug Des 2022 Jun 22. Epub 2022 Jun 22.

Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.

A seize of pleuromutilin derivatives containing amide side chains were designed and synthesized as potential antibiotics against Methicillin-resistant Staphylococcus aureus (MRSA). Among all target compounds (compounds 11-30), compound 25 was found to have the strongest antibacterial activity against MRSA (MIC = 0.5μg/mL). The result of the time-kill curves indicated that compound 25 could repress the growth of MRSA in vitro obviously (-3.72 log CFU/mL reduction). Furthermore, molecular docking studies demonstrated that compound 25 was localized in the binding pocket of 50S ribosomal subunit (ΔGb = -8.99 kcal/mol). Besides, compound 25 displayed low cytotoxicity to RAW 264.7 cells. The results suggested that compound 25 might be further developed into novel antimicrobial agent against MRSA.
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http://dx.doi.org/10.1111/cbdd.14106DOI Listing
June 2022

Constant light in early life induces fear-related behavior in chickens with suppressed melatonin secretion and disrupted hippocampal expression of clock- and BDNF-associated genes.

J Anim Sci Biotechnol 2022 Jun 21;13(1):67. Epub 2022 Jun 21.

MOE Joint International Research Laboratory of Animal Health & Food Safety, Institute of Immunology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

Background: Light management plays an important role in the growth and behavior of broiler chickens. Constant light in early post hatch stage has been a common practice in broiler industry for improving growth performance, while whether and how constant light in early life affects the behavior of broiler chickens is rarely reported.

Results: In this study, newly hatched chicks were kept in either constant (24 L:0 D, LL) or (12 L:12 D, LD) photoperiod for 7 d and then maintained in 12 L:12 D thereafter until 21 days of age. Constant light increased the average daily feed intake but not the body weight, which led to higher feed conversion ratio. Chickens in LL group exhibited fear-related behaviors, which was associated with higher corticosterone, lower melatonin and 5-HT levels. Concurrently, constant light exposure increased the mRNA expression of clock-related genes and suppressed the expression of antioxidative genes in the hippocampus. Moreover, brain derived neurotrophic factor/extracellular signal-regulated kinase (BDNF/ERK) pathway was suppressed in the hippocampus of chickens exposed to constant light in the first week post hatching.

Conclusions: These findings indicate that constant light exposure in early life suppress melatonin secretion and disrupts hippocampal expression of genes involved in circadian clock and BDNF/ERK pathway, thereby contributing to fear-related behaviors in the chicken.
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http://dx.doi.org/10.1186/s40104-022-00720-4DOI Listing
June 2022

Positive interaction between GPER and β-alanine in the dorsal root ganglion uncovers potential mechanisms: mediating continuous neuronal sensitization and neuroinflammation responses in neuropathic pain.

J Neuroinflammation 2022 Jun 21;19(1):164. Epub 2022 Jun 21.

Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Background: The pathogenesis of neuropathic pain and the reasons for the prolonged unhealing remain unknown. Increasing evidence suggests that sex oestrogen differences play a role in pain sensitivity, but few studies have focused on the oestrogen receptor which may be an important molecular component contributing to peripheral pain transduction. We aimed to investigate the impact of oestrogen receptors on the nociceptive neuronal response in the dorsal root ganglion (DRG) and spinal dorsal horn using a spared nerve injury (SNI) rat model of chronic pain.

Methods: We intrathecally (i.t.) administered a class of oestrogen receptor antagonists and agonists intrathecal (i.t.) administrated to male rats with SNI or normal rats to identify the main receptor. Moreover, we assessed genes identified through genomic metabolic analysis to determine the key metabolism point and elucidate potential mechanisms mediating continuous neuronal sensitization and neuroinflammatory responses in neuropathic pain. The excitability of DRG neurons was detected using the patch-clamp technique. Primary culture was used to extract microglia and DRG neurons, and siRNA transfection was used to silence receptor protein expression. Immunofluorescence, Western blotting, RT-PCR and behavioural testing were used to assess the expression, cellular distribution, and actions of the main receptor and its related signalling molecules.

Results: Increasing the expression and function of G protein-coupled oestrogen receptor (GPER), but not oestrogen receptor-α (ERα) and oestrogen receptor-β (ERβ), in the DRG neuron and microglia, but not the dorsal spinal cord, contributed to SNI-induced neuronal sensitization. Inhibiting GPER expression in the DRG alleviated SNI-induced pain behaviours and neuroinflammation by simultaneously downregulating iNOS, IL-1β and IL-6 expression and restoring GABAα2 expression. Additionally, the positive interaction between GPER and β-alanine and subsequent β-alanine accumulation enhances pain sensation and promotes chronic pain development.

Conclusion: GPER activation in the DRG induces a positive association between β-alanine with iNOS, IL-1β and IL-6 expression and represses GABAα2 involved in post-SNI neuropathic pain development. Blocking GPER and eliminating β-alanine in the DRG neurons and microglia may prevent neuropathic pain development.
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http://dx.doi.org/10.1186/s12974-022-02524-9DOI Listing
June 2022

[Research advances in shrub dendroecology].

Ying Yong Sheng Tai Xue Bao 2022 Jun;33(6):1699-1708

State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

The ecological value of shrub-ring data has received more and more attention. The tree-ring data of shrub species have been increasingly used to reveal growth dynamics of regional shrub vegetation and its sensitivity to climate change. Up to now, nearly 70 species of shrubs have been used in the studies of shrub dendrochronology, which considerably broadened the traditional tree-ring research network, enriched the research scope and object of dendrochronology, and certainly with great significance in revealing the characteristics of regional climate fluctuation and annual dynamics of structure, function and service for shrubland ecosystems. In this study, we systematically collected dendrochronological studies based on shrub species during the 1996-2021, and reviewed research progress in four main subfields (physiology, climatology, ecology and hydrology) in dendrochronology for shrub species. The characteristics of shrub growth and xylem anatomy under different environmental stresses were expounded. The main limiting factors for shrub growth in different climatic conditions and the history of regional climate fluctuations based on shrub-ring data were revealed. The individual growth and population dynamics of shrub species driven by climate and the changes in ecosystems caused by non-climatic factors were assessed, and the reconstructions of regional hydrological histories were compiled using tree-ring data of shrub species. Under the context of global warming, dendrochronological studies of shrub species in China should pay more attention to the responses of shrub species radial growth to drought stress under different moisture conditions in the semi-arid and arid regions and the transforming feature of distribution pattern and climate response sensitivity for shrub species under the background of climate change.
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http://dx.doi.org/10.13287/j.1001-9332.202206.006DOI Listing
June 2022

[Temporal and spatial variations of net primary productivity (NPP) and its climate driving effect in the Qinghai-Tibet Plateau, China from 2000 to 2020].

Ying Yong Sheng Tai Xue Bao 2022 Jun;33(6):1533-1538

Joint Laboratory of Eco-Meteorology, Chinese Academy of Meteorological Sciences and Zhengzhou University, Zhengzhou 450001, China.

Qinghai-Tibet Plateau is a "climate change laboratory" for China and the world. Driven by climate change, net primary productivity (NPP) in the Qinghai-Tibet Plateau has significant variations. Using the data of normalized difference vegetation index, digital elevation, annual precipitation, and annual temperature, we explored the temporal and spatial variation characteristics of NPP and its correlation with climate factors on the Qinghai Tibet Plateau from 2000 to 2020. The results showed that NPP of the Qinghai-Tibet Plateau increased significantly from 2000 to 2020, with an increase rate of 1.67 g C·m·a. The NPP was significantly positively correlated with temperature and precipitation. The climate trend of warming and wetting was an important driving force to promote the significant increase of NPP. The increases of NPP would continue if the climate become warmer and wetter.
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http://dx.doi.org/10.13287/j.1001-9332.202206.025DOI Listing
June 2022

Sugar transporter TaSTP3 activation by TaWRKY19/61/82 enhances stripe rust susceptibility in wheat.

New Phytol 2022 Jun 21. Epub 2022 Jun 21.

State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

Sugar efflux from host plants is essential for pathogen survival and proliferation. Sugar transporter-mediated redistribution of host sugar contributes to the outcomes of plant-pathogen interactions. However, few studies have focused on how sugar translocation is strategically manipulated during host colonization. To elucidate this question, the wheat sugar transport protein (STP) TaSTP3 responding to Puccinia striiformis f. sp. tritici (Pst) infection was characterized for sugar transport properties in Saccharomyces cerevisiae and potential role during Pst infection by RNA interference and overexpression in wheat. In addition, the transcription factors regulating TaSTP3 expression was further determined. Our results showed that TaSTP3 is localized to the plasma membrane and functions as a sugar transporter of hexose and sucrose. TaSTP3 confers enhanced wheat susceptibility to Pst and overexpression of TaSTP3 resulted in increased sucrose accumulation and transcriptional suppression of defense-related genes. Furthermore, TaWRKY19, TaWRKY61 and TaWRKY82 were identified as positive transcriptional regulators of TaSTP3 expression. Our findings reveal that Pst-induced sugar transporter TaSTP3 is transcriptionally activated by TaWRKY19/61/82 and faci litates wheat susceptibility to stripe rust possibly through elevated sucrose concentration, and suggest TaSTP3 as a strong target for engineering wheat resistance to stripe rust.
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http://dx.doi.org/10.1111/nph.18331DOI Listing
June 2022

LncRNA HIF1A-AS2 modulated by HPV16 E6 regulates apoptosis of cervical cancer cells via P53/caspase9/caspase3 axis.

Cell Signal 2022 Jun 18:110390. Epub 2022 Jun 18.

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China. Electronic address:

Background: Plentiful evidence proves that lncRNAs play a crucial role in tumor development. However, the function and mechanism that were mediated by lncRNA HIF1A-AS2 in cervical cancer remain unclear.

Methods: The lncRNA HIF1A-AS2 was identified via high-throughput microarray analysis of three HPV 16-positive cervical squamous cell carcinoma (CSCC) samples and three HPV-negative normal controls. The expression of HIF1A-AS2 was detected by qRT-PCR in clinical tissues and cancer cells. In vitro and in vivo assays were performed through downregulation or upregulation of HIF1A-AS2. The possible mechanisms of HIF1A-AS2 in cervical cancer cells were explored by western blot, flow cytometric analysis and rescue assays.

Results: HIF1A-AS2 was significantly increased in cervical cancer tissue, and in the HPV- positive cervical cancer cells. Further investigation showed that the inhibition of HIF1A-AS2 suppressed cell proliferation, migration, invasion, and induced apoptosis, while up-regulation of HIF1A-AS2 revealed opposite results. In terms of mechanism, we found that HIF1A-AS2 was mediated by HPV16 E6 and regulated cell apoptosis via P53/caspase 9/caspase 3 axis.

Conclusion: Our findings demonstrate that HIF1A-AS2 functions as a carcinogenic lncRNA that promotes tumor development, and serves as a candidate prognostic factor, which may contribute to the treatment of cervical cancer.
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http://dx.doi.org/10.1016/j.cellsig.2022.110390DOI Listing
June 2022

Impaired regulation of MMP2/16-MLCK3 by miR-146a-5p increased susceptibility to myocardial ischemic injury in aging mice.

Cardiovasc Res 2022 Jun 21. Epub 2022 Jun 21.

Guangdong Key Laboratory of Genome Stability and Human Disease Prevention, Department of Pathophysiology, Shenzhen University Health Science Center, Shenzhen, China.

Aims: Aging impairs cardiac function and increases susceptibility to myocardial ischemic injury. Cardiac myosin light chain kinase (MLCK3) phosphorylates cardiac myosin regulatory light chain (MLC2), controlling sarcomere organization and cardiomyocyte contraction. Dysregulation of MLCK3 and phosphorylated MLC2 (p-MLC2) contributes to heart failure after myocardial infarction (MI). We aimed at exploring how the MLCK3-p-MLC2 axis changes in aging hearts post MI and at investigating the underlying regulatory mechanisms.

Methods And Results: We generated adult (3 months) and aged (30 months) MI mouse models to compare their cardiac performance, and then detected MLCK3 expression and MLC2 activity. Aging increased the size of MI-induced infarctions and promoted cardiac contractile dysfunction. Furthermore, MLCK3 expression and MLC2 activity increased in adult hearts after MI, but not in aged hearts. miR-146a was found consistently increased in adult and aged hearts post-MI. Mechanistic analyses performed in vitro demonstrated that miR-146a-5p downregulated matrix metalloprotease (MMP)2/16 expression in cardiomyocytes. This downregulation in turn increased MLCK3 expression and MLC2 activity. However, miR-146a-5p failed to regulate the MMP2/16-MLCK3-p-MLC2 axis in senescent cardiomyocytes or in cardiac miR-146a conditional knockout mice, with the latter experiencing an exacerbated deterioration of cardiac function post-MI.

Conclusion: These results suggest that increase of MLCK3 and p-MLC2 contents through decreasing MMP2/16 by miR-146a-5p represents a compensatory mechanism that can protect cardiac contractile function after MI. Aging impairs this miR-146a-5p-regulated MMP2/16-MLCK3-p-MLC2 contractile axis, leading to compromised contractile function and increased susceptibility to heart failure.
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http://dx.doi.org/10.1093/cvr/cvac104DOI Listing
June 2022

Isolating the net effect of multiple government interventions with an extended Susceptible-Exposed-Infectious-Recovered (SEIR) framework: empirical evidence from the second wave of COVID-19 pandemic in China.

BMJ Open 2022 Jun 20;12(6):e060996. Epub 2022 Jun 20.

School of Civil Engineering, Northeast Forestry University, Harbin, China

Objective: By using a data-driven statistical approach, we isolated the net effect of multiple government interventions that were simultaneously implemented during the second wave of COVID-19 pandemic in China.

Design, Data Sources And Eligibility Criteria: We gathered epidemiological data and government interventions data of nine cities with local outbreaks during the second wave of COVID-19 pandemic in China. We employed the Susceptible-Exposed-Infectious-Recovered (SEIR) framework model to analyse the different pathways of transmission between cities with government interventions implementation and those without. We introduced new components to the standard SEIR model and investigated five themes of government interventions against COVID-19 pandemic.

Data Extraction And Synthesis: We extracted information including study objective, design, methods, main findings and implications. These were tabulated and a narrative synthesis was undertaken given the diverse research designs, methods and implications.

Results: Supported by extensive empirical validation, our results indicated that the net effect of some specific government interventions (including masks, environmental cleaning and disinfection, tracing, tracking and 14-day centralised quarantining close contacts) had been significantly underestimated in the previous investigation. We also identified important moderators and mediators for the effect of certain government interventions, such as closure of shopping mall and restaurant in the medium-risk level areas, etc. Linking the COVID-19 epidemiological dynamics with the implementation timing of government interventions, we detected that the earlier implementation of some specific government interventions (including targeted partial lockdown, tracing, tracking and 14-day centralised quarantining close contacts) achieved the strongest and most timely effect on controlling COVID-19, especially at the early period of local outbreak.

Conclusions: These findings provide important scientific information for decisions regarding which and when government interventions should be implemented to fight against COVID-19 in China and beyond. The proposed analytical framework is useful for policy-making in future endemic and pandemic as well.
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http://dx.doi.org/10.1136/bmjopen-2022-060996DOI Listing
June 2022

Phylogenomics and evolutionary history of Oreocnide (Urticaceae) shed light on recent geological and climatic events in SE Asia.

Mol Phylogenet Evol 2022 Jun 17:107555. Epub 2022 Jun 17.

Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China. Electronic address:

Climate change and geological events have long been known to shape biodiversity, implying that these can likewise be viewed from a biological perspective. To study whether plants can shed light on this, and how they responded to climate change there, we examined Oreocnide, a genus widely distributed in SE Asia. Based on broad geographic sampling with genomic data, we employed an integrative approach of phylogenomics, molecular dating, historical biogeography, and ecological analyses. We found that Oreocnide originated in mainland East Asia and began to diversify ∼6.06 Ma, probably in response to a distinct geographic and climatic transition in East Asia at around that time, implying that the last important geological change in mainland SE Asia might be 1 Ma older than previously suggested. Around four immigration events to the islands of Malesia followed, indicating that immigration from the mainland could be an underestimated factor in the assembly of biotic communities in the region. Two detected increases of diversification rate occurred 3.13 and 1.19 Ma, which strongly implicated climatic rather than geological changes as likely drivers of diversification, with candidates being the Pliocene intensification of the East Asian monsoons, and Pleistocene climate and sea level fluctuations. Distribution modelling indicated that Pleistocene sea level and climate fluctuations were inferred to enable inter-island dispersal followed by allopatric separation, underpinning radiation in the genus. Overall, our study, based on multiple lines of evidence, linked plant diversification to the most recent climatic and geological events in SE Asia. We highlight the importance of immigration in the assembly and diversification of the SE Asian flora, and underscore the utility of plant clades, as independent lines of evidence, for reconstructing recent climatic and geological events in the SE Asian region.
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http://dx.doi.org/10.1016/j.ympev.2022.107555DOI Listing
June 2022

Application of 3D Simulation Software in Chemotherapy and Hepatoblastoma Surgery in Children.

Front Surg 2022 1;9:908381. Epub 2022 Jun 1.

Department of Pediatric Surgery, Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, China.

Purpose: This study aims to explore the clinical value of a computer-assisted surgery system (Hisense CAS) in hepatoblastoma (HB) surgery in children after neoadjuvant chemotherapy.

Patients And Methods: The clinical medical records of children with HB treated after neoadjuvant chemotherapy at the Affiliated Hospital of Qingdao University from January 2016 to January 2019 were analyzed retrospectively.

Results: A total of 21 children were enrolled in this study, including 13 boys and 8 girls. All cases successfully underwent three-dimensional (3D) reconstruction of the liver and tumor using Hisense CAS, simulated hepatectomy, and hepatectomy according to the preoperative operation plan. There were twelve cases of right hemihepatectomy, four cases of right trefoil hepatectomy, one case of left lobe hepatectomy, and three cases of middle lobe hepatectomy, and one case of V and VI segment hepatectomy. All children recovered well after the operation. The follow-up ranged from 5 months to 3 years. One child died of systemic metastasis 8 months after the operation. One child received one course of chemotherapy after the operation. Due to the serious reaction to the chemotherapy, the family refused further treatment and follow-up. The remaining 19 children had no complications or recurrence.

Conclusion: Hisense CAS can clearly and intuitively display the position and shape of the HB before and after chemotherapy and its relationship with the intrahepatic pipeline system and accurately evaluate the changes in tumor volume and the distance between important blood vessels, which is conducive to the operator selecting the best operation opportunity, timely formulating the best operation plan and implementing individualized and accurate liver tumor resection.
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http://dx.doi.org/10.3389/fsurg.2022.908381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200229PMC
June 2022

Using machine learning to aid treatment decision and risk assessment for severe three-vessel coronary artery disease.

J Geriatr Cardiol 2022 May;19(5):367-376

State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Three-vessel disease (TVD) with a SYNergy between PCI with TAXus and cardiac surgery (SYNTAX) score of ≥ 23 is one of the most severe types of coronary artery disease. We aimed to take advantage of machine learning to help in decision-making and prognostic evaluation in such patients.

Methods: We analyzed 3786 patients who had TVD with a SYNTAX score of ≥ 23, had no history of previous revascularization, and underwent either coronary artery bypass grafting (CABG) or percutaneous coronary intervention (PCI) after enrollment. The patients were randomly assigned to a training group and testing group. The C4.5 decision tree algorithm was applied in the training group, and all-cause death after a median follow-up of 6.6 years was regarded as the class label.

Results: The decision tree algorithm selected age and left ventricular end-diastolic diameter (LVEDD) as splitting features and divided the patients into three subgroups: subgroup 1 (age of ≤ 67 years and LVEDD of ≤ 53 mm), subgroup 2 (age of ≤ 67 years and LVEDD of > 53 mm), and subgroup 3 (age of > 67 years). PCI conferred a patient survival benefit over CABG in subgroup 2. There was no significant difference in the risk of all-cause death between PCI and CABG in subgroup 1 and subgroup 3 in both the training data and testing data. Among the total study population, the multivariable analysis revealed significant differences in the risk of all-cause death among patients in three subgroups.

Conclusions: The combination of age and LVEDD identified by machine learning can contribute to decision-making and risk assessment of death in patients with severe TVD. The present results suggest that PCI is a better choice for young patients with severe TVD characterized by left ventricular dilation.
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http://dx.doi.org/10.11909/j.issn.1671-5411.2022.05.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170909PMC
May 2022

Study on the Expression of -1,3-N-acetylglucosaminyltransferase 3 in Gastric Cancer and the Mechanism Promoting Gastric Cancer Progression Based on the Extraction Method of Nanomagnetic Beads.

J Biomed Nanotechnol 2022 Mar;18(3):677-692

Department of Oncology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, PR China.

The oncogenic role of -1,3-N-acetylglucosaminyltransferase 3 (B3GNT3) in several cancers is well documented. However, the expression, function, and mechanism of B3GNT3 in gastric cancer (GC) remain to be investigated. Here, we extracted RNA using the nanomagnetic bead method and investigated B3GNT3 expression in GC and its mechanism for promoting malignant progression of GC using bioinformatics, quantitative reverse transcription-polymerase chain reaction (qPCR), and western blot (WB). The results showed that the upregulation of B3GNT3 expression was positively related to original T phase, lymph node metastasis, and TNM stage but negatively related to GC prognosis. Meanwhile, the knockdown of the B3GNT3 gene significantly suppressed the growth and infiltration of GC cells. In addition, B3GNT3 promoted the malignant progression of GC cells by upregulating EphA2 transcription and subsequently activating the PI3K/AKT pathway. This work reveals for the first time the upregulation and protumor role of B3GNT3 in GC and highlights the potential clinical applications of B3GNT3/EphA2/AKT signaling in GC diagnosis, treatment, and prognosis prediction.
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http://dx.doi.org/10.1166/jbn.2022.3296DOI Listing
March 2022

Unprocessed wheat γ-gliadin reduces gluten accumulation associated with the endoplasmic reticulum stress and elevated cell death.

New Phytol 2022 Jun 17. Epub 2022 Jun 17.

Frontiers Science Center for Molecular Design Breeding and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China.

Along with increasing demands for high yields, elite processing quality, and improved nutrient value in wheat, concerns have emerged around the effects of gluten in wheat-based foods on human health. However, knowledge about the mechanisms regulating gluten accumulation is still largely unexplored. We report the identification and characterization of a wheat low gluten protein 1 (lgp1) mutant that shows extremely low levels of gliadins and glutenins. The lgp1 mutation in a single γ-gliadin gene causes defective signal peptide cleavage, resulting in the accumulation of excessive amount of unprocessed γ-gliadin and a reduced level of gluten, which alters the endoplasmic reticulum (ER) structure, forms the autophagosome-like structures, leads to the delivery of seed storage proteins to extracellular space and a reduction in starch biosynthesis. Physiologically, these effects trigger the ER stress and cell death. This study unravels a unique mechanism that unprocessed γ-gliadin reduces gluten accumulation associated with the ER stress and elevated cell death in wheat. Moreover, the reduced gluten level in the lgp1 mutant makes it a good candidate for specific diets addressed to patients with diabetes or kidney diseases.
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http://dx.doi.org/10.1111/nph.18316DOI Listing
June 2022

Correction to: Trends and associated factors in the uptake of HIV testing among female sex workers in Sino-Vietnam border areas in Guangxi, China: a cross-sectional study.

BMC Infect Dis 2022 Jun 15;22(1):551. Epub 2022 Jun 15.

Guangxi Key Laboratory of AIDS Prevention and Treatment, School of Public Health, Guangxi Medical University, Nanning, 530021, Guangxi, China.

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http://dx.doi.org/10.1186/s12879-022-07521-0DOI Listing
June 2022

Organic donor-acceptor heterojunctions for high performance circularly polarized light detection.

Nat Commun 2022 Jun 15;13(1):3454. Epub 2022 Jun 15.

Beijing National Laboratory for Molecular Sciences, Institute of Chemistry Chinese Academy of Sciences, 100190, Beijing, China.

Development of highly efficient and stable lateral organic circularly polarized light photodetector is a fundamental prerequisite for realization of circularly polarized light integrated applications. However, chiral semiconductors with helical structure are usually found with intrinsically low field-effect mobilities, which becomes a bottleneck for high-performance and multi-wavelength circularly polarized light detection. To address this problem, here we demonstrate a novel strategy to fabricate multi-wavelength circularly polarized light photodetector based on the donor-acceptor heterojunction, where efficient exciton separation enables chiral acceptor layer to provide differentiated concentration of holes to the channel of organic field-effect transistors. Benefitting from the low defect density at the semiconductor/dielectric interface, the photodetectors exhibit excellent stability, enabling current roll-off of about 3-4% over 500 cycles. The photocurrent dissymmetry value and responsivity for circularly polarized light photodetector in air are 0.24 and 0.28 A W, respectively. We further demonstrate circularly polarized light communication based on a real-time circularly polarized light detector by decoding the light signal. As the proof-of-concept, the results hold the promise of large-scale circularly polarized light integrated photonic applications.
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http://dx.doi.org/10.1038/s41467-022-31186-7DOI Listing
June 2022
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