Publications by authors named "Jie Li"

5,898 Publications

  • Page 1 of 1

Effect of Sleep-Disordered Breathing During Rapid Eye Movement Sleep and Non-Rapid Eye Movement sleep on Acute Ischemic Stroke.

J Stroke Cerebrovasc Dis 2021 Jun 12;30(8):105913. Epub 2021 Jun 12.

Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China; Department of Neurology, Taicang Affiliated Hospital of Soochow University, The first People's Hospital of Taicang, Taicang 215400, China; Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience, Soochow University, Suzhou 215123, China. Electronic address:

Objectives: Sleep-disordered breathing adversely impacts stroke outcomes. We investigated whether sleep-disordered breathing during rapid eye movement sleep and non-rapid eye movement sleep differentially influenced stroke outcomes.

Materials And Methods: Acute ischemic stroke patients who finished polysomnography within 14 days of stroke onset from April 2010 to August 2018 were reviewed. Patients were divided into four groups according to apnea-hypopnea index during rapid eye movement sleep and non-rapid eye movement sleep. The modified Rankin Scale was used to evaluate short-term outcome. During January and April 2019, another follow-up was performed for long-term outcomes, including stroke-specific quality-of-life scale, modified Rankin Scale, stroke recurrence and death.

Results: Of 140 patients reviewed, 109 were finally recruited. Although patients with sleep-disordered breathing during non-rapid eye movement sleep only and with sleep-disordered breathing during both rapid eye movement sleep and non-rapid eye movement sleep had higher apnea-hypopnea indices and more disrupted sleep structures, short-term and long-term outcomes did not significantly different between four groups. In Logistic regression analysis, apnea-hypopnea index (p = 0.013, OR 1.023, 95%CI 1.005-1.042) was found independently associated with short-term outcome. Rapid eye movement sleep latency (p = 0.045, OR 0.994, 95%CI 0.987-1.000) was found independently associated with quality of life. Apnea-hypopnea indices during rapid eye movement sleep or non-rapid eye movement sleep were not significantly associated with short-term or long-term outcomes.

Conclusions: Apnea-hypopnea index is an independent risk factor of short-term outcome of acute ischemic stroke while sleep-disordered breathing during rapid eye movement sleep and non-rapid eye movement sleep do not affect stroke outcomes differently.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2021.105913DOI Listing
June 2021

C and L band room-temperature continuous-wave InP-based microdisk lasers grown on silicon.

Opt Lett 2021 Jun;46(12):2836-2839

Quantum-dot (QD) and quantum-dash (QDash) have been shown to be promising gain materials for lasers directly grown on Si due to their better tolerance to crystal defects and thermal stability. Here we report optically pumped InP-based InAs QDash microdisk lasers (MDLs) directly grown on on-axis (001) Si. To the best of our knowledge, this is the first demonstration of room-temperature continuous-wave lasing of a QDash MDL on Si in the C band and L band. To the best of our knowledge, the lowest threshold of around 400 µW and highest operation temperature of 323 K have been achieved. An analysis of experimental results shows that the dominant lasing wavelength of MDLs varies with the thickness and diameter of the MDLs. Our demonstration shows potential application of MDLs for multi-channel operation in densely integrated Si-photonics.
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http://dx.doi.org/10.1364/OL.420106DOI Listing
June 2021

The Use of Aerosolized Medications in Adult Intensive Care Unit Patients: A Prospective, Multicenter, Observational, Cohort Study.

J Aerosol Med Pulm Drug Deliv 2021 Jun 14. Epub 2021 Jun 14.

Department of Respiratory and Critical Care Medicine, West China Medical Center, Sichuan University, Chengdu, China.

Only limited data are available on the real-life clinical utilization of aerosolized medications in intensive care unit (ICU) patients. Exploring the utilization of aerosolized medications in the ICU may contribute to develop appropriate education and improve the quality of aerosol therapy. A 2-week, prospective, multicenter, observational, cohort study was conducted to record how the aerosolized medications were utilized in the Chinese ICUs, including indications, medications used in solo or combination, dosage, and side-effects in adult patients. A total of 1006 patients from 28 ICUs were enrolled, of which 389 (38.7%) received aerosol therapy. The most common indications for aerosol therapy were difficulty in secretion management (23.1%) and chronic obstructive pulmonary disease exacerbation (18.5%). The combination of inhaled corticosteroids and short-acting muscarinic antagonist was the most commonly used medication (19.5%, 76/389). Ninety-two percent (358/389) of the patients did not have any side effects during aerosol therapy. More patients in the group with mechanical ventilation received bronchodilators than spontaneous breathing patients (81.3% vs. 55.5%,  < 0.001), and more patients who breathed spontaneously through a tracheostomy received mucus-regulating agents than other patients (70% vs. 37.9%,  = 0.004). In mainland China, more than one-third of adult ICU patients received aerosol therapy. Medications utilized during aerosol therapy were variable in patients with different respiratory support. To promote appropriate use of aerosolized medications, high-quality randomized, controlled trials and clinical guidance on aerosolized medication indications and dosing are needed to improve clinical outcomes.
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http://dx.doi.org/10.1089/jamp.2021.0004DOI Listing
June 2021

Photoinduced Reversible Semicrystalline-to-Amorphous State Transitions of Stereoregular Azopolyesters.

Angew Chem Int Ed Engl 2021 Jun 15. Epub 2021 Jun 15.

Dalian University of Technology, State Key Laboratory of Fine Chemicals, CHINA.

Precise control over both the chirality and sequence of a synthetic polymer is critical for manipulating its material performance. Herein, we report the synthesis of a variety of isotactic azobenzene-based polyesters (azopolyesters) with main-chain chirality via highly enantioselective resolution copolymerization of racemic azobenzene-containing epoxides with cyclic anhydrides, using an enantiopure binuclear catalyst system. All the prepared polyesters bearing trans -azobenzene moieties were found to be typical semicrystalline materials with melting temperatures in the range of 153 to 231 °C, while the corresponding isotactic cis -azopolyesters were amorphous. The azobenzene groups in the copolymers exhibited reversible trans -to- cis and cis -to- trans photoisomerization, upon irradiation with 365 and 450 nm light, respectively. These observations demonstrate that the crystallinity of isotactic azopolyesters can be manipulated via photoinduced reversible isomerization. In addition, mixing isotactic trans -polyesters with different enantiomeric configurations in a 1:1 mass ratio afforded crystalline stereocomplexes, for which the crystalline behaviors differed significantly from those of the component enantiomer. Also, photoinduced reversible transitions between semicrystalline and amorphous states were observed in various stereocomplexes of isotactic trans -azopolyesters, being similar to the isotactic azopolyesters themselves.
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http://dx.doi.org/10.1002/anie.202104750DOI Listing
June 2021

ginal Article Unintended Consequences of Expanding Electronic Pathology Reporting: The Inverse Relationship Between Data Completeness and Data Quality.

J Registry Manag 2020 ;47(3):122-126

Background: In 2016, the New Jersey State Cancer Registry (NJSCR) began expanding electronic laboratory reporting. As a result, the number of electronic pathology reports (EPRs) submitted to NJSCR increased markedly from 2015 to 2017. EPRs are more likely to contain incomplete or missing race than North American Association of Central Cancer Registry (NAACCR) abstracts from hospitals and physician offices. NJSCR staff conduct follow-back for additional information for laboratory-only cases, but response rates are poor, the process is lengthy, and laboratory reports often do not include physician information.

Purpose: To assess the impact of increased EPR on the quality of race data.

Methods: NJSCR data sets created 24 months after the end of the diagnosis year-with data that were more than 98% complete-were used to calculate the percent of EPR-only cases by primary site and the percent of cases with unknown race. We calculated the relative risk of unknown race by site, compared to all sites, and used Spearman's ρ to assess the correlation between EPR-only cases and unknown race.

Results: While the percent of cases with unknown race was within the standards for NAACCR Gold Certification (3%), it varied by cancer site. Sites less likely to be reported by hospitals had higher rates of unknown race in the 24-month data set: prostate, leukemia, melanoma, bladder. After follow-back and death clearance activities, ≥36 months after the diagnosis year, the percent of cases with unknown race is reduced, although the impact varies by cancer site.

Conclusion: Race-specific incidence rates for certain cancer types may be artificially depressed in the 24-month data set due to the unavailability of race for the increasing number of laboratory-only cases. While follow-back activities help to improve the collection of race data over time, these new values are not available until a revised data set is released. The higher proportion of unknown or other race in the 24-month data set impacts the accuracy of reporting the burden and trends of cancer by race. In addition, cases with unknown race may be ineligible for inclusion in cancer surveillance research studies.
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June 2021

Accurate prediction of chemical shifts for aqueous protein structure on "Real World" data.

Chem Sci 2020 Mar 3;11(12):3180-3191. Epub 2020 Mar 3.

Pitzer Center for Theoretical Chemistry, University of California Berkeley CA 94720 USA

Here we report a new machine learning algorithm for protein chemical shift prediction that outperforms existing chemical shift calculators on realistic data that is not heavily curated, nor eliminates test predictions . Our UCBShift predictor implements two modules: a transfer prediction module that employs both sequence and structural alignment to select reference candidates for experimental chemical shift replication, and a redesigned machine learning module based on random forest regression which utilizes more, and more carefully curated, feature extracted data. When combined together, this new predictor achieves state-of-the-art accuracy for predicting chemical shifts on a randomly selected dataset without careful curation, with root-mean-square errors of 0.31 ppm for amide hydrogens, 0.19 ppm for Hα, 0.84 ppm for C', 0.81 ppm for Cα, 1.00 ppm for Cβ, and 1.81 ppm for N. When similar sequences or structurally related proteins are available, UCBShift shows superior native state selection from misfolded decoy sets compared to SPARTA+ and SHIFTX2, and even without homology we exceed current prediction accuracy of all other popular chemical shift predictors.
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http://dx.doi.org/10.1039/c9sc06561jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152569PMC
March 2020

A dual band spin-selective transmission metasurface and its wavefront manipulation.

Nanoscale 2021 Jun 15. Epub 2021 Jun 15.

Key Laboratory of Opto-Electronics Information Technology (Tianjin University), Ministry of Education, School of Precision Instruments and Opto-Electronics Engineering, Tianjin University, Tianjin, 300072, China.

Chiral metasurfaces which can achieve different optical responses for left-handed and right-handed circularly polarized (CP) light have been proposed. Most of the research studies on chiral metasurfaces focus on improving circular dichroism (CD) and realizing dynamic manipulation of the chiro-optical response. However, there have only been a few reports on the multi-band chiro-optical response. Here, we propose an all-silicon chiral meta-atom which can realize spin-selective transmission in a dual band. In addition, a terahertz metasurface with spin-selective transmission through phase arrangement is designed by using chiral meta-atoms satisfying a gradient geometric phase. Under left-hand circularly polarized (LCP) incidence, the metasurface generates a focused right-hand circularly polarized (RCP) beam which is focused at a distance of 4.8 mm from the exit surface of the metasurface. Our work broadens the concept of metasurface design and may attract more researchers' attention on the applications of chiral metasurfaces.
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http://dx.doi.org/10.1039/d1nr02624kDOI Listing
June 2021

A simplified repair method for adult Ebstein's anomaly.

J Card Surg 2021 Jun 14. Epub 2021 Jun 14.

Department of Cardiovascular and Thoracic Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu, China.

Ebstein's anomaly (EA) is a rare but difficult to manage congenital heart disease with a wide spectrum of clinical manifestations. We present a simplified repair method which combines the plication of the atrialized right ventricle, tricuspid leaflet repair and ring annuloplasty. This method is suitable for older adult EA patients with progressive right heart dysfunction symptoms. Compared with complex repair methods (such as Cone reconstruction) this simplified repair method can reduce surgical risk, and achieve mild or less tricuspid regurgitation with acceptable long-term effects compared with prosthetic valve replacement.
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http://dx.doi.org/10.1111/jocs.15726DOI Listing
June 2021

A traceless linker for aliphatic amines that rapidly and quantitatively fragments after reduction.

Chem Sci 2020 Aug 12;11(33):8973-8980. Epub 2020 Aug 12.

Department of Bioengineering, University of California Berkeley University Avenue Berkeley CA 94720 USA

Reduction sensitive linkers (RSLs) have the potential to transform the field of drug delivery due to their ease of use and selective cleavage in intracellular environments. However, despite their compelling attributes, developing reduction sensitive self-immolative linkers for aliphatic amines has been challenging due to their poor leaving group ability and high p values. Here a traceless self-immolative linker composed of a dithiol-ethyl carbonate connected to a benzyl carbamate (DEC) is presented, which can modify aliphatic amines and release them rapidly and quantitatively after disulfide reduction. DEC was able to reversibly modify the lysine residues on CRISPR-Cas9 with either PEG, the cell penetrating peptide Arg, or donor DNA, and generated Cas9 conjugates with significantly improved biological properties. In particular, Cas9-DEC-PEG was able to diffuse through brain tissue significantly better than unmodified Cas9, making it a more suitable candidate for genome editing in animals. Furthermore, conjugation of Arg to Cas9 with DEC was able to generate a self-delivering Cas9 RNP that could edit cells without transfection reagents. Finally, conjugation of donor DNA to Cas9 with DEC increased the homology directed DNA repair (HDR) rate of the Cas9 RNP by 50% in HEK 293T cell line. We anticipate that DEC will have numerous applications in biotechnology, given the ubiquitous presence of aliphatic amines on small molecule and protein therapeutics.
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http://dx.doi.org/10.1039/d0sc00929fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163433PMC
August 2020

Deep learning applications in automatic segmentation and reconstruction in CT-based cervix brachytherapy.

J Contemp Brachytherapy 2021 Jun 13;13(3):325-330. Epub 2021 May 13.

Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Radiation Oncology Key Laboratory of Sichuan Province, Chengdu, China.

Purpose: Motivated by recent advances in deep learning, the purpose of this study was to investigate a deep learning method in automatic segment and reconstruct applicators in computed tomography (CT) images for cervix brachytherapy treatment planning.

Material And Methods: U-Net model was developed for applicator segmentation in CT images. Sixty cervical cancer patients with Fletcher applicator were divided into training data and validation data according to ratio of 50 : 10, and another 10 patients with Fletcher applicator were employed to test the model. Dice similarity coefficient (DSC) and 95 percentile Hausdorff distance (HD95) were used to evaluate the model. Segmented applicator coordinates were calculated and applied into RT structure file. Tip error and shaft error of applicators were evaluated. Dosimetric differences between manual reconstruction and deep learning-based reconstruction were compared.

Results: The averaged overall 10 test patients' DSC, HD95, and reconstruction time were 0.89, 1.66 mm, and 17.12 s, respectively. The average tip error was 0.80 mm, and the average shaft error was less than 0.50 mm. The dosimetric differences between manual reconstruction and automatic reconstruction were 0.29% for high-risk clinical target volume (HR-CTV) D, and less than 2.64% for organs at risk D at a scenario of doubled maximum shaft error.

Conclusions: We proposed a deep learning-based reconstruction method to localize Fletcher applicator in three-dimensional CT images. The achieved accuracy and efficiency confirmed our method as clinically attractive. It paves the way for the automation of brachytherapy treatment planning.
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http://dx.doi.org/10.5114/jcb.2021.106118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170523PMC
June 2021

Prevalence of Thyroid Nodules in China: A Health Examination Cohort-Based Study.

Front Endocrinol (Lausanne) 2021 26;12:676144. Epub 2021 May 26.

Department of Endocrine and Breast Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: Thyroid nodules are a common clinical problem and some are potentially cancerous; however, little is known about the prevalence of thyroid nodules in China. The objective of this study was to investigate the prevalence of thyroid nodules in a healthy Chinese population.

Methods: We reviewed electronic medical records of 13,178,313 participants from 30 provinces and regions who received health examinations and underwent thyroid ultrasound at Meinian Onehealth Healthcare in 2017. Among them, 6,192,357 were excluded based on predefined criteria. All thyroid nodules were diagnosed by ultrasonography, and standardized protocols were adopted for data collection, quality control, and data management.

Results: A total of 6,985,956 participants (mean age: 42.1 ± 13.1 years) were included in this study. The overall prevalence of thyroid nodules was 36.9% (95% CI, 35.7%-38.1%; age- and sex-standardized prevalence 38.0% [95% CI, 37.0%-39.1%]). The prevalence of thyroid nodules in females (44.7% [95% CI, 43.4%-45.9%], age-standardized prevalence: 45.2% [95% CI, 44.1%-46.4%]) was significantly higher than that in males (29.9% [95% CI, 28.8%-31.0%], age-standardized prevalence 31.2% [95% CI, 30.1%-32.2%]; < 0.001). The prevalence of thyroid nodules decreased from <18 to 25 years, while increased with age over 25 years old. The top three provinces with the highest prevalence of thyroid nodules were Jilin (47.6%), Liaoning (44.8%), and Shandong (43.9%), whereas Guizhou (23.9%), Chongqing (26.2%), and Shaanxi (26.4%) had the lowest prevalence. Females had more than 10% higher rates of thyroid nodules than males in all included provinces and regions, except for Tianjin (8.0%). Based on the geographical regions of China, the northeast had the highest prevalence (46.8% [95% CI, 44.1%-49.2%]), whereas northwest had the lowest prevalence (28.9% [95% CI, 26.9%-31.6%]. Based on multivariable logistic regression analysis, factors including age, gender, body mass index, systolic blood pressure, diastolic blood pressure, uric acid, fasting blood glucose, triglycerides, high-density lipoproteins, and low-density lipoproteins were significantly associated with the presence of thyroid nodules.

Conclusion: This study provides the first nationwide analysis of the prevalence of thyroid nodules in China. Our results showed that the prevalence of thyroid nodules was high in health screening Chinese people with regional-specific patterns.
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http://dx.doi.org/10.3389/fendo.2021.676144DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188053PMC
May 2021

An electrographic AV optimization for the maximum integrative atrioventricular and ventricular resynchronization in CRT.

BMC Cardiovasc Disord 2021 Jun 10;21(1):288. Epub 2021 Jun 10.

Department of Cardiology, The First Affiliated Hospital of Sun Yat-Sen University, No. 58, Zhongshan 2nd Rd, Guangzhou, 510080, Guangdong, People's Republic of China.

Background: Atrioventricular (AV) delay could affect AV and ventricular synchrony in cardiac resynchronization therapy (CRT). Strategies to optimize AV delay according to optimal AV synchrony (AV) or ventricular synchrony (AV) would potentially be discordant. This study aimed to explore a new AV delay optimization algorithm guided by electrograms to obtain the maximum integrative effects of AV and ventricular resynchronization (opt-AV).

Methods: Forty-nine patients with CRT were enrolled. AV was measured through the Ritter method. AV was obtained by yielding the narrowest QRS. The opt-AV was considered to be AV or AV when their difference was < 20 ms, and to be the AV delay with the maximal aortic velocity-time integral between AV and AV when their difference was > 20 ms.

Results: The results showed that sensing/pacing AV (SAV/PAV) were correlated with atrial activation time (P/P) (P < 0.05). Sensing/pacing AV (SAV/PAV) was correlated with the intrinsic AV conduction time (As-Vs/Ap-Vs) (P < 0.01). The percentages of patients with more than 20 ms differences between SAV/PAV and SAV/PAV were 62.9% and 57.1%, respectively. Among them, opt-AV was linearly correlated with SAV/PAV and SAV/PAV The sensing opt-AV (opt-SAV) = 0.1 × SAV + 0.4 × SAV + 70 ms (R = 0.665, P < 0.01) and the pacing opt-AV (opt-PAV) = 0.25 × PAV + 0.5 × PAV + 30 ms (R = 0.560, P < 0.01).

Conclusion: The SAV/PAV and SAV/PAV were correlated with the atrial activation time and the intrinsic AV conduction interval respectively. Almost half of the patients had a > 20 ms difference between SAV/PAV and SAV/PAV. The opt-AV could be estimated based on electrogram parameters.
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http://dx.doi.org/10.1186/s12872-021-02096-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193898PMC
June 2021

Dual Inorganic Sacrificial Template Synthesis of Hierarchically Porous Carbon with Specific N Sites for Efficient Oxygen Reduction.

ACS Appl Mater Interfaces 2021 Jun 11. Epub 2021 Jun 11.

School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, Hunan, China.

It is still a challenge to achieve efficiently controlled preparation of functional oxygen reduction reaction (ORR) carbon electrocatalysts with multi-preferred structures (hierarchically porous networks and specific carbon-nitrogen bonds) from carbohydrate-containing small molecules via simple one-step pyrolysis. Based on the step-by-step spontaneous gas-foaming strategy, we successfully prepare 3D hierarchically porous networks with tunable N sites (N/N ≈ 1:1) by pyrolyzing diverse carbohydrates (glucose, maltose, and cyclodextrin) using nonmetal-metal dual inorganic sacrificial templates. In situ evaporation templates can simplify the procedure of the experiments and avoid the active site loss compared with traditional hard templates. Crucially, dual inorganic sacrificial templates can induce abundant defects and microscopic pore structures (the specific surface area increased from 922.403 to 1898.792 m·g) and tunable N sites compared with single nonmetal sacrificial templates. The regulatory mechanism of dual inorganic templates on N sites (N/N ≈ 1:1) is independent of the polymeric state of carbohydrate precursors or even the carbonization condition of the pyrolysis process. A series of carbon materials prepared by this strategy all have ORR-preferred structures and exhibit low ORR overpotentials compared with Pt/C. For instance, the Zn-air battery with βCD-DSC-950-1 exhibits an open-circuit potential of 1.51 V and a peak power density of 180.89 mW·cm, higher than those of Pt/C (1.47 V, 174.94 mW·cm). In general, the conversion of carbohydrate-containing small molecules to functional carbon materials provides a new strategy for the development of carbonaceous electrocatalysts.
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http://dx.doi.org/10.1021/acsami.1c04942DOI Listing
June 2021

A versatile nanoagent for multimodal imaging-guided photothermal and anti-inflammatory combination cancer therapy.

Biomater Sci 2021 Jun 10. Epub 2021 Jun 10.

Department of Ultrasonography, The Third Affiliated Hospital of Southern Medical University, Academy of Orthopedics, Guangzhou 510515, Guangdong Province, P. R. China.

Photothermal therapy (PTT) has drawn great attention in cancer treatment because of its minimal invasiveness and high spatiotemporal selectivity, but it still encounters severe obstacles like heat-resistance, metastasis and recurrence. A key reason for the treatment failure is the highly inflammatory tumor microenvironment caused by hyperthermia. A simultaneous anti-inflammatory therapy alongside the PTT has great potential for overcoming the drawbacks of PTT; however, it has been less reported and further study is urgently needed. In addition, as many inorganic photothermal agents have no inherent imaging capability, diagnostic strategies should be introduced to help identify cancerous lesions and find the best treatment time period for PTT. Herein, we developed a versatile theranostic nanoagent (named T-lipos-CPAuNCs) for synergistic multimodal imaging-guided photothermal/anti-inflammatory cancer therapy. Perfluorohexane (PFH) loaded AuNCs and the anti-inflammatory drug celecoxib were encapsulated into the tumor-targeting cyclic Arg-Gly-Asp (cRGD) peptide modified liposomes to form T-lipos-CPAuNCs. The T-lipos-CPAuNCs accumulated in the tumor tissue and selectively targeted the cancer cells, and converted photo to thermal energy under near-infrared (NIR) laser irradiation to kill the cancer cells by PTT. The high temperature further accelerated the release of celecoxib to exert an anti-inflammatory effect, while on the other hand led to liquid to gas phase transition of PFH to facilitate ultrasound (US) imaging. The T-lipos-CPAuNCs also exhibited photoacoustic (PA) imaging capability. In vitro and in vivo experiments established that under the guidance of multimodal imaging, T-lipos-CPAuNCs significantly suppressed the tumor growth by PTT and prevented tumor metastasis with non-apparent tumor inflammation. The developed theranostic nanosystem (T-lipos-CPAuNCs) shows great potential for PA/US multimodal imaging guided photothermal/anti-inflammatory combination cancer therapy.
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http://dx.doi.org/10.1039/d1bm00576fDOI Listing
June 2021

Clinicopathological features and lymph node and distant metastasis patterns in patients with gastroenteropancreatic mixed neuroendocrine-non-neuroendocrine neoplasm.

Cancer Med 2021 Jun 10. Epub 2021 Jun 10.

Department of Gastrointestinal Oncology, Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, Peking University Cancer Hospital and Institute, Beijing, China.

Objective: Owing to its rarity and heterogeneity, the biological behavior and optimal therapeutic management of mixed neuroendocrine-non-neuroendocrine neoplasm (MiNEN) have not been established. Herein, we aimed to evaluate the clinicopathological characteristics and metastatic patterns of MiNEN.

Methods: Continuous clinicopathological data of MiNEN patients treated at our hospital were retrospectively collected and analyzed.

Results: This study had enrolled 169 patients since January 2010 to January 2020. Pathological components were assessed in 129 patients with MiNEN (76.3%), and a focal (non-)neuroendocrine component was observed in 40 patients (23.7%; <30% of the tumor). Among the enrolled patients, 80 underwent surgical removal of the primary tumor and lymph nodes (LNs), and 34 with distant metastasis underwent biopsy of both primary tumor and metastatic lesions. In patients with LN metastasis, 68.8% (55/80) exhibited a pure component of either neuroendocrine (NE) or adenocarcinoma/squamous carcinoma (AS) in metastatic LNs, while 20% (16/80) showed different components in different LNs, and only 11.2% (9/80) exhibited both NE and AS components in the same LN. In patients with distant metastases, 26.5% (9/34) possessed coexisting NE and AS components in the distant metastases, 70.6% (24/34) were regarded as a pure NE component, and 2.9% (1/34) were comprised of a pure AS component.

Conclusion: Lymph node and distant metastases exhibited distinct metastatic patterns in patients with MiNEN. The major pathological component in regional LNs may have influenced the proportion of the two components within the primary tumor, but distant metastases were dominated by the NE component.
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http://dx.doi.org/10.1002/cam4.4031DOI Listing
June 2021

Differences in clinical characteristics and liver injury between suspected and confirmed COVID-19 patients in Jingzhou, Hubei Province of China.

Medicine (Baltimore) 2021 May;100(19):e25913

Department of Gastroenterology, First Hospital of Yangtze University.

Abstract: To evaluate the clinical characteristics and liver injury in coronavirus disease 2019 (COVID-19) patients, and analyze the differences between suspected and confirmed COVID-19 patients, this retrospective study was performed on 157 COVID-19 patients and 93 suspected patients who were ultimately excluded from COVID-19 (control patients). Differences in clinical characteristics and liver injury between suspected and confirmed COVID-19 patients were analyzed. Age, male sex, fever, chest tightness and dyspnea were related to the severity of COVID-19. C-reactive protein (CRP) and D-dimer may be predictors of the severity of COVID-19. Computed tomography (CT) played an important role in the screening of COVID-19 and the evaluation of disease severity. Multiple factors may cause liver injury in COVID-19 patients. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) may be more likely to cause liver injury than common respiratory infectious diseases. Age, temperature (T), white blood cell (WBC), lymphocytes (LY), hematocrit (HCT), CRP, and finger pulse oxygen saturation (SpO2) may correlate with liver function impairment and may predict the occurrence and severity of liver function impairment. Some therapeutic drugs (like glucocorticoid) may be involved in the liver function impairment of COVID-19 patients. Most liver function indices improved significantly after active treatment. Although COVID-19 and other common respiratory infectious diseases share some clinical characteristics, COVID-19 has its own characteristics.
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http://dx.doi.org/10.1097/MD.0000000000025913DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133163PMC
May 2021

Early Decline of Neuron-Specific Enolase during Neuroblastoma Chemotherapy is a Predictive Factor of Clinical Outcome.

Pediatr Hematol Oncol 2021 Jun 9:1-13. Epub 2021 Jun 9.

Department of Pediatric Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.

High risk neuroblastoma (HR-NB) remains one of the most difficult-to-treat pediatric cancers. However, although current risk-stratification is based on multiple pretreatment criteria, HR-NB remains a significant heterogeneity. We examined 60 patients with HR-NB for a median follow-up time of 28 months. We examined the serum neuronspecific enolase (NSE) levels of each chemo cycle, using the survival receiver operating characteristic (survivalROC) method to assess the prognostic power of NSE levels at variant chemo points. We demonstrated that serum NSE was associated with systemic tumor burden. NSE after the third chemo cycle (C3) (C3NSE) was significantly higher in patients who eventually showed cancer relapse or progression. C3NSE had independent prognostic significance for event-free survival (EFS) but not for overall survival (OS) in multivariate cox analysis. SurvivalROC prompted that the C3NSE is a prognostic marker of HR-NB, which had good discrimination for 2- and 3-year EFS with AUC 0.734 and 0.729, respectively. However, its prognositc value for 2- and 3- year OS declined progressively. C3 is the optimal point to predict EFS. Patients whose C3 serum NSE remain at higher level need to undergo more intensive treatment as early as possible to resist recurrence.
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http://dx.doi.org/10.1080/08880018.2021.1894277DOI Listing
June 2021

Seasonal Variations in the Characteristics of Microbial Community Structure and Diversity in Atmospheric Particulate Matter from Clean Days and Smoggy Days in Beijing.

Microb Ecol 2021 Jun 8. Epub 2021 Jun 8.

School of Environment, Beijing Normal University, Beijing, 100875, China.

Microorganisms are an important part of atmospheric particulate matter and are closely related to human health. In this paper, the variations in the characteristics of the chemical components and bacterial communities in PM and PM grouped according to season, pollution degree, particle size, and winter heating stage were studied. The influence of environmental factors on community structure was also analyzed. The results showed that seasonal variations were significant. NO contributed the most to the formation of particulate matter in spring and winter, while SO contributed the most in summer and autumn. The community structures in summer and autumn were similar, while the community structure in spring was significantly different. The dominant phyla were similar among seasons, but their proportions were different. The dominant genera were no-rank_c_Cyanobacteria, Acidovorax, Escherichia-Shigella and Sphingomonas in spring; Massilia, Bacillus, Acinetobacter, Rhodococcus, and Brevibacillus in summer and autumn; and Rhodococcus in winter. The atmospheric microorganisms in Beijing mainly came from soil, water, and plants. The few pathogens detected were mainly affected by the microbial source on the sampling day, regardless of pollution level. RDA (redundancy analysis) showed that the bacterial community was positively correlated with the concentration of particulate matter and that the wind speed in spring was positively correlated with NO levels, NH levels, temperature, and relative humidity in summer and autumn, but there was no clear consistency among winter samples. This study comprehensively analyzed the variations in the characteristics of the airborne bacterial community in Beijing over one year and provided a reference for understanding the source, mechanism, and assessment of the health effects of different air qualities.
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http://dx.doi.org/10.1007/s00248-021-01764-1DOI Listing
June 2021

Resolving the graft ischemia-reperfusion injury during liver transplantation at the single cell resolution.

Cell Death Dis 2021 Jun 8;12(6):589. Epub 2021 Jun 8.

Organ Transplant Center, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510080, P. R. China.

Ischemia-reperfusion injury (IRI) remains the major reason for impaired donor graft function and increased mortality post-liver transplantation. The mechanism of IRI involves multiple pathophysiological processes and numerous types of cells. However, a systematic and comprehensive single-cell transcriptional profile of intrahepatic cells during liver transplantation is still unclear. We performed a single-cell transcriptome analysis of 14,313 cells from liver tissues collected from pre-procurement, at the end of preservation and 2 h post-reperfusion. We made detailed annotations of mononuclear phagocyte, endothelial cell, NK/T, B and plasma cell clusters, and we described the dynamic changes of the transcriptome of these clusters during IRI and the interaction between mononuclear phagocyte clusters and other cell clusters. In addition, we found that TNFAIP3 interacting protein 3 (TNIP3), specifically and highly expressed in Kupffer cell clusters post-reperfusion, may have a protective effect on IRI. In summary, our study provides the first dynamic transcriptome map of intrahepatic cell clusters during liver transplantation at single-cell resolution.
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http://dx.doi.org/10.1038/s41419-021-03878-3DOI Listing
June 2021

Clinical Phenotype and Mutation Spectrum of Alzheimer's Disease with Causative Genetic Mutation in a Chinese Cohort.

Curr Alzheimer Res 2021 Jun 8. Epub 2021 Jun 8.

Department of Neurology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences/ PekingUnion Medical College, Beijing, 100730, China.

Background: Alzheimer's disease with a causative genetic mutation (AD-CGM) is an un- common form, characterized by a heterogeneous clinical phenotype and variations in the genotype of racial groups affected.

Objective: We aimed to systemically describe the phenotype variance and mutation spectrum in the large sample size of the Peking Union Medical College Hospital (PUMCH) cohort, Beijing, China.

Method: Next-generation sequencing (NGS) was carried out in 1108 patients diagnosed with dementia. A total of 40 Han Chinese patients with three AD gene mutations were enrolled. A systemic review of all the patients was performed, including clinical history, neurocognitive assessment, brain magnetic resonance imaging, and cerebrospinal fluid (CSF) biomarkers.

Results: We studied the following gene mutation variants: 12 AβPP, 13 PSEN1, and 9 PSEN2, and 23 among them were novel. Most of them were early-onset, but PSEN1 mutation carriers had the youngest onset age. The commonest symptoms were similar to those of AD, including an amnestic syndrome, followed by psychiatric symptoms and movement disorder. On MRI, parietal and posterior temporal atrophy was prominent in PSEN1 and PSEN2 mutation carriers, while AβPP mutation carriers had more vascular changes. The CSF biomarkers profile was indistinguishable from sporadic AD.

Conclusion: We identified a small group of AD-CGM subjects representing 3.6% among more than 1000 demented patients in the PUMCH cohort. These subjects usually presented with early-onset de- mentia and exhibited significant clinical and genetic heterogeneity. Identification required complete screening of genetic mutations using NGS. Although family history was usually present, we found non-familial cases of all three genetic mutations.Recent Advances in Anti-Infective Drug Discovery.
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http://dx.doi.org/10.2174/1567205018666210608120339DOI Listing
June 2021

The Inhibitory Effect of Emotional Conflict Control on Memory Retrieval.

Neuroscience 2021 Jun 5;468:29-42. Epub 2021 Jun 5.

Department of Rehabilitation, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China. Electronic address:

Evidence is mounting that emotional conflict is mainly resolved by the rostral anterior cingulate inhibiting the processing of emotional distractors. However, this theory has not been verified from the perspective of memory retrieval. This experiment aimed to explore the offline effect of emotional conflict processing on memory retrieval. We adopted a modified encoding-retrieval paradigm to explore this issue. Participants' electroencephalography (EEG) signal were also collected. A face-word Stroop task was used to create the congruency factor. In addition, an old/new judgment task was used to evaluate the recognition performance. During the retrieval phase, the response time of the incongruent condition was longer and the recognition accuracy was lower compared with congruent and neutral conditions in the behavioral data. For event-related potentials (ERP), we detected two well-established old/new effects related to memory retrieval under both neutral and emotional conditions: the frontal negativity (FN400) related to familiarity-driven recognition and the late posterior negativity (LPN) related to reconstructive processing or evaluation of retrieval outcomes. More importantly, the old/new effects were missing for incongruent condition during the early stage of FN400 (300-400 ms). Besides, for LPN (700-900 ms), the old/new effects of the incongruent condition are greater than the congruent condition. The results prove that the encoding phase's emotional congruency factor has a regulatory effect on the retrieval phase's early familiarity processing and evaluation of retrieval outcomes. Our data confirm the inhibitory effect of emotional conflict control on memory retrieval and support the emotional conflict control mechanism found in previous studies.
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http://dx.doi.org/10.1016/j.neuroscience.2021.05.032DOI Listing
June 2021

Sanguisorba parviflora (Maxim.) Takeda alleviates cyclophosphamide-induced leukopenia by regulating haematopoietic cell-specific protein 1-associated protein X-1 gene expression.

J Clin Pharm Ther 2021 Jun 8. Epub 2021 Jun 8.

School of Pharmacy, Mudanjiang Medical University, Mudanjiang, China.

What Is Known And Objective: We have previously shown that the saponins of Sanguisorba parviflora (Maxim.) Takeda (Sp. T) relieved cyclophosphamide-induced myelosuppression in leukopenic mice. Haematopoietic cell-specific protein 1-associated protein X-1 (HAX-1) participated in the survival of neutrophils through the regulation of mitochondrial function. The aim of the present study was to comprehensively identify the role of HAX-1 in the mechanism of leukopenia alleviation by Sp. T.

Methods: HAX-1 gene and protein expression levels in peripheral blood neutrophils were examined using real-time quantitative reverse transcription-polymerase chain reaction, western blot and immunohistochemical assays. Neutrophil apoptosis was measured using flow cytometry. Mitochondrial function was determined via assessments of the reactive oxygen species (ROS) generation and mitochondrial membrane potential (ΔΨm) integrity levels.

Results And Discussion: The HAX-1 gene expression level in the peripheral blood neutrophils was significantly lower in patients with leukopenia than in healthy donors. The saponins of Sp. T induced HAX-1 expression and promoted myeloid progenitor cell (mEB8-ER cell) viability. HAX-1 overexpression reduced the production of ROS and maintained ΔΨm integrity. Cyclophosphamide-induced mitochondrial dysfunction and apoptosis could be abrogated by treatment with Sp. T or metformin.

What Is New And Conclusion: Our data suggest a mechanism through which Sp. T protects against chemotherapy-induced leukopenia by regulating HAX-1 gene expression in a mitochondrial-dependent manner.
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http://dx.doi.org/10.1111/jcpt.13468DOI Listing
June 2021

A novel indel within the bovine gene is associated with ovary length.

Anim Biotechnol 2021 Jun 7:1-7. Epub 2021 Jun 7.

College of Veterinary Medicine, Northwest A&F University, Yangling, China.

The ovary can generate oocytes and secrete female hormones and thus is of great significance to animal fertility. In turn, the functioning of this organ has an effect on the profit margins of the livestock breeding industry. As the development-regulating gene and target gene of miR-202, might play an important role in ovarian growth. Therefore, we hypothesized that is related to ovarian traits owing to the regulation of gonad-specific miR-202. To further investigate the connection between bovine and ovarian development, we analyzed data from 408 samples. After genotyping and analyzing three selected loci, we found that two out of the three loci (L1 and L5) were polymorphic, of which the minimum allelic frequencies were 0.417 (L1) and 0.094 (L5). Moreover, one novel indel L1 of was associated with ovarian length ( < 0.05). More specifically, individuals with II and ID genotypes have longer ovaries than those with the DD genotype. Our work shows that can be selected as a testing marker gene for animal fertility. Our findings contribute to improving the prospects of the cattle industry and the wider use of genetic techniques in breeding.
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http://dx.doi.org/10.1080/10495398.2021.1929272DOI Listing
June 2021

[A case of tuberous sclerosis complex due to a novel splicing variant of TSC2 gene].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Jun;38(6):553-556

Center for Reproductive Medicine Research, Shandong University; National Research Center for Assisted Reproductive Technology and Reproductive Genetics; Key Laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, Shandong 250001, China.

Objective: To explore the genetic basis for a patient with tuberous sclerosis complex.

Methods: Genomic DNA was extracted from peripheral blood samples from members of his family and 100 unrelated healthy controls. The proband was subjected to next-generation sequencing, and candidate variant was confirmed by multiple ligation-dependent probe amplification (MLPA) and Sanger sequencing. Reverse transcription-PCR (RT-PCR) was carried out to determine the relative mRNA expression in the proband.

Results: The patient was found to harbor a c.2355+1G>C splicing variant of the TSC2 gene. Sequencing of cDNA confirmed that 62 bases have been inserted into the 3' end of exon 21, which has caused a frameshift producing a truncated protein.

Conclusion: The novel splicing variant c.2355+1G>C of the TSC2 gene probably underlay the TSC in the proband. Above finding has expanded the variant spectrum of TSC2 and provided a basis for preimplantation genetic testing and/or prenatal diagnosis.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200211-00072DOI Listing
June 2021

Selenite Induces Cell Cycle Arrest and Apoptosis Reactive Oxygen Species-Dependent Inhibition of the AKT/mTOR Pathway in Thyroid Cancer.

Front Oncol 2021 21;11:668424. Epub 2021 May 21.

Department of Endocrinology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Thyroid cancer is the most common endocrine malignancy, and its incidence has increased in the past decades. Selenium has been shown to have therapeutic effects against several tumors. However, its role in thyroid cancer and its underlying molecular mechanism remains to be explored. In the present study, we demonstrated that sodium selenite significantly decreased cell viability and induced G0/G1 cell cycle arrest and apoptosis in thyroid cancer cells in a dose-dependent manner. Transcriptomics revealed that sodium selenite induced intracellular reactive oxygen species (ROS) by promoting oxidative phosphorylation. Increased intracellular ROS levels inhibited the AKT/mTOR signaling pathway and upregulated EIF4EBP3. Intracellular ROS inhibition by N-acetylcysteine (NAC) ameliorated the cellular effects of sodium selenite. The findings were reproduced in xenograft thyroid tumor models. Our data demonstrated that sodium selenite exhibits strong anticancer effects against thyroid cancer cells, which involved ROS-mediated inhibition of the AKT/mTOR pathway. This suggests that sodium selenite may serve as a therapeutic option for advanced thyroid cancer.
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http://dx.doi.org/10.3389/fonc.2021.668424DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176115PMC
May 2021

High Expression of and at the Resection Margin Makes Lung Cancer Survivors Susceptible to SARS-CoV-2 With Unfavorable Prognosis.

Front Oncol 2021 21;11:644575. Epub 2021 May 21.

Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Background: Coronavirus disease 2019 (COVID-19) has rapidly spread worldwide. Systematic analysis of lung cancer survivors at molecular and clinical levels is warranted to understand the disease course and clinical characteristics.

Methods: A single-center, retrospective cohort study was conducted in 65 patients with COVID-19 from Wuhan Huoshenshan Hospital, of which 13 patients were diagnosed with lung cancer. The study was conducted from February 4 to April 11, 2020.

Results: During the course of treatment, lung cancer survivors infected with severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) had shorter median time from symptom onset to hospitalization ( = 0.016) and longer clinical symptom remission time ( = 0.020) than non-cancer individuals. No differences were observed among indicators such as time from symptom onset to hospitalization and symptom remission time between medium-term and short-term survivors. The expression of ( = 0.013) and (0.001) was elevated in lung cancer survivors as compared with that in non-cancer individuals.

Conclusions: and levels were higher at resection margins of lung cancer survivors than those in normal tissues of non-cancerous individuals and may serve as factors responsible for the high susceptibility to COVID-19 among lung cancer survivors. Lung cancer patients diagnosed with COVID-19, including medium-term survivors, have worse outcomes than the general population.
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http://dx.doi.org/10.3389/fonc.2021.644575DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176089PMC
May 2021

pH-Responsive Au(i)-disulfide nanoparticles with tunable aggregation-induced emission for monitoring intragastric acidity.

Chem Sci 2020 Apr 24;11(25):6472-6478. Epub 2020 Apr 24.

Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Department of Chemistry, The Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong China

Aggregation-induced emission (AIE)-featuring Au(i) complexes are superior probes for physiological environment monitoring in living organisms owing to their excellent biocompatibility and efficient luminescent properties. However, the intrinsic obstacle of poor water stability and lack of response to biological stimuli greatly restrict their practical application in biological systems. Herein, water-stable and pH-responsive Au(i)-disulfide nanoparticles (NPs) with AIE characteristics were designed. The NPs were prepared by integrating a pH-responsive moiety, cysteine (Cys), into Au(i)-thiolate (SR) complexes, and the Au(i)-SR-Cys structure was formed through disulfide bonds. Hydrophilic Cys was located on the outer layer of the NPs, endowing the spherical NPs with high stability and remarkable monodispersity in water. In addition, Cys endowed the NPs with pH-responsive characteristics. These unique advantages enable them to hold great promise as luminescent probes to monitor intragastric acidity in an acid suppression therapy. To the best of our knowledge, this work is the first example of luminescent Au(i) materials to monitor physiological changes.
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http://dx.doi.org/10.1039/d0sc01843kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159400PMC
April 2020

Xiang Study: an association of breastmilk composition with maternal body mass index and infant growth during the first 3 month of life.

Nutr Res Pract 2021 Jun 6;15(3):367-381. Epub 2020 Oct 6.

H&H Group, Global Research and Technology Center, Guangzhou 510700, China.

Background/objectives: This study aimed to establish a mother and child cohort in the Chinese population, and investigate human breastmilk (HBM) composition and its relationship with maternal body mass index (BMI) and infant growth during the first 3 mon of life.

Subjects/methods: A total of 101 Chinese mother and infant pairs were included in this prospective cohort. Alterations in the milk macronutrients of Chinese mothers at 1 mon (T1), 2 mon (T2), and 3 mon (T3) lactation were analyzed. HBM fatty acid (FA) profiles were measured by gas chromatography (GC), and HBM proteomic profiling was achieved by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS).

Results: During the first 3 mon of lactation ( < 0.05), significant decreases were determined in the levels of total energy, fat, protein, and osteopontin (OPN), as well as ratios of long-chain saturated FA (including C16:0, C22:0 and C24:0), monounsaturated FA (including C16:1), and n-6 poly unsaturated FA (PUFA) (including C20:3n-6 and C20:4n-6, and n-6/n-3). Conversely, butyrate, C6:0 and n-3 PUFA C18:3n-3 (α-linolenic acid, ALA) were significantly increased during the first 3 mon ( < 0.05). HBM proteomic analyses distinguished compositional protein differences over time ( = 0.001). Personalized mother-infant analyses demonstrated that HBM from high BMI mothers presented increased total energy, fat, protein and OPN, and increased content of n-6 PUFA (including C18:3n-6, C20:3n-6 and n-6/n-3 ratio) as compared with low BMI mothers ( < 0.05). Furthermore, BMI of the mothers positively correlated with the head circumference (HC) of infants as well as the specific n-6 PUFA C20:3n-6 over the 3 time points examined. Infant HC was negatively associated with C18:0.

Conclusion: This study provides additional evidence to the Chinese HBM database, and further knowledge of FA function. It also helps to establish future maternal strategies that support the healthy growth and development of Chinese infants.
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http://dx.doi.org/10.4162/nrp.2021.15.3.367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155227PMC
June 2021

Isolation and Molecular Identification of the Native Microflora on Fruiting Bodies and Modeling the Growth of Dominant Microbiota ().

Front Microbiol 2021 21;12:664874. Epub 2021 May 21.

College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, China.

The objectives of this study were to isolate and identify the dominant microorganism in fruiting bodies (FVFB) and to develop kinetic models for describing its growth. The native microflora community on FVFB was isolated and identified using morphological examination and high-throughput sequencing analysis. FVFB presented complex microbial communities with dominant microorganisms being Irradiated FVFB were inoculated with the isolated strain of and cultivated at various temperatures (4, 10, 16, 20, 25, 32, and 37°C). Three primary models, namely the Huang, Baranyi and Roberts, and reparameterized Gompertz models, and three secondary models, namely the Huang square-root, Ratkowsky square-root, and Arrhenius-type models, were developed and evaluated. With the lowest values of mean square error (MSE, 0.023-0.161) and root mean square error (RMSE, 0.152-0.401) values, the reparameterized Gompertz model was more suitable to describe the growth of on FVFB than both Huang and Baranyi and Roberts models. The Ratkowsky square-root model provided more accurate estimation for the effect of temperature on the specific growth rate of . The minimum growth temperature predicted by the Ratkowsky square-root model was -7.1°C. The kinetic models developed in this study could be used to evaluate the growth behavior of on FVFB and estimate the shelf-life of FVFB.
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http://dx.doi.org/10.3389/fmicb.2021.664874DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176924PMC
May 2021

The Post-verbal Effect of Negators in Mongolian Contradictory Negations Provides Support for the Fusion Model.

Front Psychol 2021 20;12:603075. Epub 2021 May 20.

School of Psychology, Inner Mongolia Normal University, Hohhot, China.

There are two contending models regarding the processing of negation: the and the . Most previous studies have centered on negation in languages such as English and Mandarin, where negators are positioned before predicates. Mongolian, quite uniquely, is a language whose negators are post-verbal, making them natural replicas of the schema-plus-tag model. The present study aims to investigate the representation process of Mongolian contradictory negative sentences to shed light on the debate between the models, meanwhile verifying the post-verbal effect of negators. A series of experiments using the supports the fusion model: (i) Mongolian contradictory negative sentences were processed by representing the actual conditions rather than the negated state of affairs at 250 ISI (interstimulus interval of 250 ms), and (ii) despite the fact that a was measured at 250 ISI when Mongolian and Mandarin negative sentences were compared, Mongolian-Mandarin bilinguals adopted the same representational strategy for contradictory negation in both languages.
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http://dx.doi.org/10.3389/fpsyg.2021.603075DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173153PMC
May 2021