Publications by authors named "Jie Han"

544 Publications

The role of the HIF-1α/ALYREF/PKM2 axis in glycolysis and tumorigenesis of bladder cancer.

Cancer Commun (Lond) 2021 May 15. Epub 2021 May 15.

Department of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210000, P. R. China.

Background: As a rate-limiting enzyme of glycolysis, pyruvate kinase muscle isozyme M2 (PKM2) participates in tumor metabolism and growth. The regulatory network of PKM2 in cancer is complex and has not been fully studied in bladder cancer. The 5-methylcytidine (m5C) modification in PKM2 mRNA might participate in the pathogenesis of bladder cancer and need to be further clarified. This study aimed to investigate the biological function and regulatory mechanism of PKM2 in bladder cancer.

Methods: The expression of PKM2 and Aly/REF export factor (ALYREF) was measured by Western blotting, qRT-PCR, and immunohistochemistry. The bioprocesses of bladder cancer cells were demonstrated by a series of experiments in vitro and in vivo. RNA immunoprecipitation, RNA-sequencing, and dual-luciferase reporter assays were conducted to explore the potential regulatory mechanisms of PKM2 in bladder cancer.

Results: In bladder cancer, we first demonstrated that ALYREF stabilized PKM2 mRNA and bound to its m5C sites in 3'-untranslated regions. Overexpression of ALYREF promoted bladder cancer cell proliferation by PKM2-mediated glycolysis. Furthermore, high expression of PKM2 and ALYREF predicted poor survival in bladder cancer patients. Finally, we found that hypoxia-inducible factor-1alpha (HIF-1α) indirectly up-regulated the expression of PKM2 by activating ALYREF in addition to activating its transcription directly.

Conclusions: The m5C modification in PKM2 mRNA in the HIF-1α/ALYREF/PKM2 axis may promote the glucose metabolism of bladder cancer, providing a new promising therapeutic target for bladder cancer.
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http://dx.doi.org/10.1002/cac2.12158DOI Listing
May 2021

Bioinformatics-Based Analysis of the lncRNA-miRNA-mRNA Network and TF Regulatory Network to Explore the Regulation Mechanism in Spinal Cord Ischemia/Reperfusion Injury.

Front Genet 2021 27;12:650180. Epub 2021 Apr 27.

Department of Anesthesiology, The First Hospital of China Medical University, Shenyang, China.

Background: Spinal cord ischemia/reperfusion injury (SCII) is a catastrophic complication involved with cardiovascular, spine, and thoracic surgeries and can lead to paraplegia. Nevertheless, the molecular mechanism of SCII remain ill-defined.

Methods: Expression profiling (GSE138966) data were obtained from GEO database. Then, differentially expressed (DE) lncRNAs and DEmRNAs were screened out with < 0.05, and | fold change| > 1.5. Aberrant miRNAs expression in SCII was obtained from PubMed. Functional enrichment analysis of overlapping DEmRNAs between predicted mRNAs in miRDB database and DEmRNAs obtained from GSE138966 was performed using cluster Profiler R package. The lncRNA-miRNA-mRNA competitive endogenous RNA (ceRNA) network was established in light of ceRNA theory. The key lncRNAs in the ceRNA network were identified by topological analysis. Subsequently, key lncRNAs related ceRNA-pathway network and transcription factors (TFs)-mRNAs network were constructed. Simultaneously, the expression levels of hub genes were measured via qRT-PCR.

Results: The results in this study indicated that 76 miRNAs, 1373 lncRNAs, and 4813 mRNAs were differentially expressed in SCII. A SCII-related ceRNA network was constructed with 154 ncRNAs, 139 mRNAs, and 51 miRNAs. According topological analysis, six lncRNAs (NONRATT019236.2, NONRATT009530.2, NONRATT026999.2, TCONS_00032391, NONRATT023112.2, and NONRATT021956.2) were selected to establish the ceRNA-pathway network, and then two candidate hub lncRNAs (NONRATT009530.2 and NONRATT026999.2) were identified. Subsequently, two lncRNA-miRNA-mRNA regulatory axes were identified. NONRATT026999.2 and NONRATT009530.2 might involve SCII via miR-20b-5p/Map3k8 axis based on the complex ceRNA network. SP1 and Hnf4a acting as important TFs might regulate Map3k8. Furthermore, qRT-PCR results showed that the NONRATT009530.2, NONRATT026999.2, Map3k8, Hfn4a, and SP1 were significantly upregulated in SCII of rats, while the miR-20b-5p was downregulated.

Conclusion: Our results offer a new insight to understand the ceRNA regulation mechanism in SCII and identify highlighted lncRNA-miRNA-mRNA axes and two key TFs as potential targets for prevention and treatment of SCII.
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http://dx.doi.org/10.3389/fgene.2021.650180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110913PMC
April 2021

Crown ether-pillararene hybrid macrocyclic systems.

Org Biomol Chem 2021 Apr;19(15):3287-3302

Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Energy), College of Chemistry, Nankai University, Tianjin 300071, China.

A combination of Nobel macrocycle-crown ether and star macrocycle-pillararenes together in organic synthesis and material science is significant in obtaining hybrid systems, with rigid/flexible structural architecture, induced planar chirality, a negative cooperative effect and multiple fused cyclic hosts. In this review, we will discuss the synthesis/preparation of crown ether-pillararene hybrid macrocyclic systems by covalent bonds, supramolecular interactions and mechanical bonds, leading to hybrid compounds, supramolecular assemblies and mechanically interlocked molecules. The practical applications of crown ether-containing pillararenes will also be discussed in diverse areas, such as molecular recognition via fused multiple macrocycles and ion channels as well as external stimuli-responsive smart materials. We also call the attention of related researchers towards academic and technical issues about topological structures and applied functions in this fresh new fused macrocyclic field.
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http://dx.doi.org/10.1039/d1ob00222hDOI Listing
April 2021

Antimicrobial photodynamic therapy with hypocrellin B against SARS-CoV-2 infection?

Photodiagnosis Photodyn Ther 2021 Apr 20;34:102297. Epub 2021 Apr 20.

Key Laboratory of Molecular Target and Clinical Pharmacology, State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences & Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, 511436, China. Electronic address:

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http://dx.doi.org/10.1016/j.pdpdt.2021.102297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8057054PMC
April 2021

Benzoate ester as a new species for supramolecular chiral assembly.

Soft Matter 2021 Apr 21. Epub 2021 Apr 21.

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, P. R. China.

In this work, a benzoate ester molecule, dodecamethylnonacosane-2,28-diyl dibenzoate (DMNDB), has been discovered as a new species that aggregates into chiral nano-assemblies. In the tetrahydrofuran (THF)/water system, the benzoate ester, DMNDB, could self-assemble into left-handed twisted nanowires, and the most suitable THF/water volume ratio to obtain uniform twisted nanowires was 3 : 7. The driving forces of assembly and the molecular packing type in assemblies for the twisted nanowires were explored, and a possible assembly mechanism was proposed to understand the generation of chiral assemblies. Interestingly, the left-handed nanowires could cross-link and immobilize the solvent in the isopropanol (iPrOH)/water (2 : 8) system to form chiral gels. When the iPrOH/water ratio was increased to 6 : 4, the left-handed nanowires as structural units were found to evolve to right-handed nanofibers. Accordingly, the intermolecular interactions and the molecular packing type also changed with the solvent ratio. What is more, the xerogel could be obtained by drying the gel and left-handed twisted nanowires could form in the THF/water system again, showing the recyclability of chiral nanoassemblies. Also, these DMNDB chiral nanostructures exhibited potential for application in enantioselective separation by co-assembling with tetra-aniline.
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http://dx.doi.org/10.1039/d1sm00188dDOI Listing
April 2021

Hydrogen sulfide restores cardioprotective effects of remote ischemic preconditioning in aged rats HIF-1α/Nrf2 signaling pathway.

Korean J Physiol Pharmacol 2021 May;25(3):239-249

Department of Cardiovascular, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.

The present study explored the therapeutic potential of hydrogen sulfide (HS) in restoring aging-induced loss of cardioprotective effect of remote ischemic preconditioning (RIPC) along with the involvement of signaling pathways. The left hind limb was subjected to four short cycles of ischemia and reperfusion (IR) in young and aged male rats to induce RIPC. The hearts were subjected to IR injury on the Langendorff apparatus after 24 h of RIPC. The measurement of lactate dehydrogenase, creatine kinase and cardiac troponin served to assess the myocardial injury. The levels of HS, cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), nuclear factor erythroid 2-related factor 2 (Nrf2), and hypoxia-inducible factor (HIF-1α) were also measured. There was a decrease in cardioprotection in RIPC-subjected old rats in comparison to young rats along with a reduction in the myocardial levels of HS, CBS, CSE, HIF-1α, and nuclear: cytoplasmic Nrf2 ratio. Supplementation with sodium hydrogen sulfide (NaHS, an HS donor) and l-cysteine (HS precursor) restored the cardioprotective actions of RIPC in old hearts. It increased the levels of HS, HIF-1α, and Nrf2 ratio without affecting CBS and CSE. YC-1 (HIF-1α antagonist) abolished the effects of NaHS and l-cysteine in RIPC-subjected old rats by decreasing the Nrf2 ratio and HIF-1α levels, without altering HS.The late phase of cardioprotection of RIPC involves an increase in the activity of HS biosynthetic enzymes, which increases the levels of HS to upregulate HIF-1α and Nrf2. HS has the potential to restore aging-induced loss of cardioprotective effects of RIPC by upregulating HIF-1α/Nrf2 signaling.
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http://dx.doi.org/10.4196/kjpp.2021.25.3.239DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050610PMC
May 2021

P300/CBP inhibition sensitizes mantle cell lymphoma to PI3Kδ inhibitor idelalisib.

Acta Pharmacol Sin 2021 Apr 13. Epub 2021 Apr 13.

Drug Discovery and Design Center, The Center for Chemical Biology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.

Mantle cell lymphoma (MCL) is a lymphoproliferative disorder lacking reliable therapies. PI3K pathway contributes to the pathogenesis of MCL, serving as a potential target. However, idelalisib, an FDA-approved drug targeting PI3Kδ, has shown intrinsic resistance in MCL treatment. Here we report that a p300/CBP inhibitor, A-485, could overcome resistance to idelalisib in MCL cells in vitro and in vivo. A-485 was discovered in a combinational drug screening from an epigenetic compound library containing 45 small molecule modulators. We found that A-485, the highly selective catalytic inhibitor of p300 and CBP, was the most potent compound that enhanced the sensitivity of MCL cell line Z-138 to idelalisib. Combination of A-485 and idelalisib remarkably decreased the viability of three MCL cell lines tested. Co-treatment with A-485 and idelalisib in Maver-1 and Z-138 MCL cell xenograft mice for 3 weeks dramatically suppressed the tumor growth by reversing the unsustained inhibition in PI3K downstream signaling. We further demonstrated that p300/CBP inhibition decreased histone acetylation at RTKs gene promoters and reduced transcriptional upregulation of RTKs, thereby inhibiting the downstream persistent activation of MAPK/ERK signaling, which also contributed to the pathogenesis of MCL. Therefore, additional inhibition of p300/CBP blocked MAPK/ERK signaling, which rendered maintaining activation to PI3K-mTOR downstream signals p-S6 and p-4E-BP1, thus leading to suppression of cell growth and tumor progression and eliminating the intrinsic resistance to idelalisib ultimately. Our results provide a promising combination therapy for MCL and highlight the potential use of epigenetic inhibitors targeting p300/CBP to reverse drug resistance in tumor.
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http://dx.doi.org/10.1038/s41401-021-00643-2DOI Listing
April 2021

Biocide-tolerance and antibiotic-resistance in community environments and risk of direct transfers to humans: Unintended consequences of community-wide surface disinfecting during COVID-19?

Environ Pollut 2021 Apr 3;283:117074. Epub 2021 Apr 3.

Department of Environmental Science and Engineering, College of Earth and Environmental Sciences, Lanzhou University, Lanzhou, 730000, PR China.

During the current pandemic, chemical disinfectants are ubiquitously and routinely used in community environments, especially on common touch surfaces in public settings, as a means of controlling the virus spread. An underappreciated risk in current regulatory guidelines and scholarly discussions, however, is that the persisting input of chemical disinfectants can exacerbate the growth of biocide-tolerant and antibiotic-resistant bacteria on those surfaces and allow their direct transfers to humans. For COVID-19, the most commonly used disinfecting agents are quaternary ammonium compounds, hydrogen peroxide, sodium hypochlorite, and ethanol, which account for two-thirds of the active ingredients in current EPA-approved disinfectant products for the novel coronavirus. Tolerance to each of these compounds, which can be either intrinsic or acquired, has been observed on various bacterial pathogens. Of those, mutations and horizontal gene transfer, upregulation of efflux pumps, membrane alteration, and biofilm formation are the common mechanisms conferring biocide tolerance in bacteria. Further, the linkage between disinfectant use and antibiotic resistance was suggested in laboratory and real-life settings. Evidence showed that substantial bacterial transfers to hands could effectuate from short contacts with surrounding surfaces and further from fingers to lips. While current literature on disinfectant-induced antimicrobial resistance predominantly focuses on municipal wastes and the natural environments, in reality the community and public settings are most severely impacted by intensive and regular chemical disinfecting during COVID-19 and, due to their proximity to humans, biocide-tolerant and antibiotic-resistant bacteria emerged in these environments may pose risks of direct transfers to humans, particularly in densely populated urban communities. Here we highlight these risk factors by reviewing the most pertinent and up-to-date evidence, and provide several feasible strategies to mitigate these risks in the scenario of a prolonging pandemic.
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http://dx.doi.org/10.1016/j.envpol.2021.117074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019131PMC
April 2021

Photodynamic therapy with curcumin for combating SARS-CoV-2.

Photodiagnosis Photodyn Ther 2021 Apr 6;34:102284. Epub 2021 Apr 6.

Key Laboratory of Molecular Target and Clinical Pharmacology, State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences & Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, 511436, China.

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http://dx.doi.org/10.1016/j.pdpdt.2021.102284DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022585PMC
April 2021

Smarter cures to combat COVID-19 and future pathogens: a review.

Environ Chem Lett 2021 Apr 2:1-13. Epub 2021 Apr 2.

CNRS, IRD, INRAE, Coll France, CEREGE, Aix-Marseille University, 13100 Aix en Provence, France.

Prevention is better than cure. A milestone of the anthropocene is the emergence of a series of epidemics and pandemics often characterized by the transmission of a pathogen from animals to human in the past two decades. In particular, the coronavirus disease 2019 (COVID-19) has made a profound impact on emergency responding and policy-making in a public health crisis. Classical solutions for controlling the virus, such as travel restrictions, lockdowns, repurposed drugs and vaccines, are socially unpopular and medically limited by the fast mutation and adaptation of the virus. This is exacerbated by microbial resistance to therapeutic drugs and the slowness of vaccine development. In other words, microbial pathogens are somehow 'smarter' and faster than us, thus calling for more intelligent cures to combat future pandemics. Here, we compare therapeutics for COVID-19 such as synthetic drugs, vaccines, antibodies and phages. We present the strength and limitations of antibiotic and antiviral drugs, vaccines, and antibody-based therapeutics. We describe smarter, cheaper and preventive cures such as bacteriophages, food medicine using probiotics and prebiotics, sports, healthy diet, music, yoga, Tai Chi, dance, reading, knitting, cooking and outdoor activities. Some of these preventive cures have been intuitively developed since thousands of years ago, as illustrated by the fascinating similarity of the Chinese characters for 'music' and 'herbal medicine.'
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http://dx.doi.org/10.1007/s10311-021-01224-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017513PMC
April 2021

Ultraefficiently Calming Cytokine Storm Using TiCT MXene.

Small Methods 2021 Jan 18:2001108. Epub 2021 Jan 18.

Faculty of Science University of Technology Sydney Sydney NSW 2007 Australia.

During the global outbreak of COVID-19 pandemic, "cytokine storm" conditions are regarded as the fatal step resulting in most mortality. Hemoperfusion is widely used to remove cytokines from the blood of severely ill patients to prevent uncontrolled inflammation induced by a cytokine storm. This article discoveres, for the first time, that 2D TiCT MXene sheet demonstrates an ultrahigh removal capability for typical cytokine interleukin-6. In particular, MXene shows a 13.4 times higher removal efficiency over traditional activated carbon absorbents. Molecular-level investigations reveal that MXene exhibits a strong chemisorption mechanism for immobilizing cytokine interleukin-6 molecules, which is different from activated carbon absorbents. MXene sheet also demonstrates excellent blood compatibility without any deleterious side influence on the composition of human blood. This work can open a new avenue to use MXene sheets as an ultraefficient hemoperfusion absorbent to eliminate the cytokine storm syndrome in treatment of severe COVID-19 patients.
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http://dx.doi.org/10.1002/smtd.202001108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995020PMC
January 2021

Evaluation of premature ventricular contractions originating from periprosthetic aortic valve regions.

J Cardiovasc Electrophysiol 2021 May 19;32(5):1519. Epub 2021 Mar 19.

Department of Cardiovascular Medicine, Mayo Clinic, Rochester, Minnesota, USA.

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http://dx.doi.org/10.1111/jce.14990DOI Listing
May 2021

Association of polymorphisms of innate immunity-related genes and tuberculosis susceptibility in Mongolian population.

Hum Immunol 2021 Apr 7;82(4):232-239. Epub 2021 Mar 7.

Institutes of Biomedical Sciences, Shanxi University, Taiyuan 030006, China. Electronic address:

Backgrounds: Genetic polymorphism of the toll-like receptor 2, 4 (TLR2, TLR4) and natural resistance-associated macrophage protein 1 (NRAMP1) genes may affect host immune response to Mycobacterium tuberculosis (Mtb) and lead to the variation of susceptibility to tuberculosis (TB) in humans. However, the association of single nucleotide polymorphisms (SNP) in these genes and the susceptibility to TB in Mongolian population has not been investigated.

Methods: We conducted a genetic association study including 197 Mongolian TB patients and 217 Mongolian healthy controls in Inner Mongolia, China. DNA of blood samples was extracted and genotyped for 5 SNPs in TLR4, 4 SNPs in TLR2 and 5 SNPs in NRAMP1 by next-generation sequencing. A logistic regression was performed and odds ratios (OR) with 95% confidence intervals (CI) were calculated to estimate the risk at TB by each SNP.

Results: The most significant locus associated with the susceptibility to TB was TLR4 rs11536889. The frequency for allele C of TLR4 rs11536889 was 16.0% in TB patients and 23.5% in healthy controls, respectively. Rs11536889 C/C genotype of TLR4 was significantly associated with the low susceptibility against TB compared to G/G genotype in the dominant model (OR 0.62, 95% CI 0.41-0.94).

Conclusions: The TLR4 rs11536889 polymorphisms might be an indicative of the low susceptibility to TB in Mongolian population, which provides valuable information for the generation of effective strategy or measurement against TB in Mongolian population.
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http://dx.doi.org/10.1016/j.humimm.2021.02.008DOI Listing
April 2021

Development of an ultrasensitive spectrophotometric method for carmine determination based on fluorescent carbon dots.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2021 May 8;38(5):731-740. Epub 2021 Mar 8.

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225001, PR China.

A high-efficiency spectrophotometric method based on nitrogen-doped fluorescent carbon dots (N-FCDs) was developed for the ultrasensitive determination of carmine (CRM) in foodstuffs. The N-FCDs were fabricated via a one-pot hydrothermal method with -phenylenediamine as the starting material. The detection principle was based on the fluorescence quenching effect of N-FCDs by CRM, where their interaction was due to the inner filter effect (IFE) and static quenching. A good linear relationship was established for CRM detection in a concentration range of 0.1-10.0 μM with a detection limit as low as 11.2 nM. The proposed method achieved satisfactory results for CRM determination in commercial food products with recoveries better than 98.6% and relative standard deviations (RSDs) less than 4.07%. The method established in this study was simple, ultrasensitive and reliable for rapid detecting CRM in a food matrix, which could be potentially used as a useful sensing agent for the analysis of additive food colourants.
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http://dx.doi.org/10.1080/19440049.2021.1889045DOI Listing
May 2021

Clinical significance of serum hepcidin in the diagnosis and treatment of patients with anemia of chronic disease: a meta-analysis.

Authors:
Jie Han Kai Wang

Biomarkers 2021 Jun 2;26(4):296-301. Epub 2021 Mar 2.

Department of Hepatology, Qilu Hospital of Shandong University and Hepatology Institute of Shandong University, Jinan, China.

Objective: To systematically evaluate the value of serum hepcidin in the diagnosis of Anaemia of Chronic Disease (ACD) in order to provide appropriate treatment.

Methods: Literature search was performed in PubMed, EMbase, Cochrane Library, CNKI, CBMdisc and CSPD till Jan, 2020. Studies using hepcidin assay for the diagnosis of ACD were included. Two researchers selected the literature according to the pre-defined inclusion and exclusion criteria. Meta-analysis was performed using Stata 15.0.

Results: A total of 10 studies were included, including 739 patients with 402 ACD patients. Heterogeneity test results suggest that there is no statistical heterogeneity between the included studies and Meta- analysis was performed using a fixed-effects model. Results showed that serum hepcidin levels in patients with ACD combined with SEN, SPE, PLR, NLR, and Diagnostic OR were 0.94 [95% CI (0.90, 0.96)], 0.85 [95% CI (0.81, 0.88)], 6.1 [95% CI (4.8, 7.6)], 0.08 [95% CI (0.05, 0.12)] and 81 [95% CI (47, 139)] respectively. The area under the SROC curve (AUC) value was 0.91.

Conclusion: Serum hepcidin assay is a valuable method to diagnose ACD in patients. However, due to the limitations of the quantity and quality of the research, the above conclusions need more research to verify.
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http://dx.doi.org/10.1080/1354750X.2021.1893812DOI Listing
June 2021

Efficacy and Safety of Qishen Yiqi Dripping Pill for Heart Failure With Preserved Ejection Fraction: A Systematic Review and Meta-Analysis.

Front Pharmacol 2020 9;11:626375. Epub 2021 Feb 9.

Department of Cardiology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.

The number of heart failure with preserved ejection fraction (HFpEF) patients is increasing year by year, yet all western medicines currently used for heart failure have been shown to be ineffective for HFpEF. Qishen Yiqi Dripping Pill is one of the commonly drugs for the treatment of heart failure in China. In recent years, some clinical studies found that it has curative effect on HFpEF. To evaluate the efficacy and safety of Qishen Yiqi Dripping Pill in treatment of HFpEF. Databases including CNKI, Wanfang, VIP, CBM, PubMed, Web of Science, The Cochrane Library and EMbase were searched from their inception to May 2020 to screen relevant randomized controlled trials. The "risk of bias" evaluation tool in the Cochrane Handbook was used to evaluate the quality of the included studies. RevMan 5.3 software was used for meta-analysis. Eight studies meeting the criteria were included, with a total of 895 patients. The results of meta-analysis showed that compared with western medicine alone, combination of western medicine and Qishen Yiqi Dripping Pill can further increase the quotient of early diastolic mitral inflow velocity and late diastolic mitral inflow velocity (E/A) in patients with HFpEF [mean difference (MD) = 0.20, 95% CI (0.14, 0.26), < 0.000 01], decrease the quotient of early diastolic mitral inflow velocity and mitral annular tissue velocity (E/e') [MD = -2.50, 95% CI (-3.18, -1.82), < 0.000 01], decrease brain natriuretic peptide (BNP) [MD = -151.83, 95% CI (-245.78, -57.89), = 0.002], increase cardiac function improvement rate [relative risk (RR) = 1.30, 95% CI (1.11, 1.52), = 0.001], and increase six-minutes walking distance (6-MWD) [MD = 64.75, 95% CI (22.65, 106.85), = 0.003]. Four studies reported the occurrence of adverse reactions, among which three studies reported no adverse reactions and one study reported three patients with mild adverse reactions in the intervention group. Current evidence suggests that Qishen Yiqi Dripping Pill may be effective in the treatment of HFpEF. However, due to the low quality of the included studies, lack of placebo control, large heterogeneity among different studies, and great possibility of publication bias, the results of our review should be evaluated with more prudence, more high-quality clinical studies are needed to verify the conclusion in the future. In addition, the safety of Qishen Yiqi Dripping Pill remains uncertain, further assessment is required in the future.
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http://dx.doi.org/10.3389/fphar.2020.626375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900630PMC
February 2021

Effect of Coronavirus Disease 2019 on Patients with Bladder Cancer in China.

Urol Int 2021 Feb 24:1-3. Epub 2021 Feb 24.

Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China,

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http://dx.doi.org/10.1159/000512895DOI Listing
February 2021

A simple paper-based colorimetric analytical device for rapid detection of Enterococcus faecalis under the stress of chlorophenols.

Talanta 2021 Apr 5;225:121966. Epub 2020 Dec 5.

Department of Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China; Department of Science, School of Science and Technology, The Open University of Hong Kong, Good Shepherd Street, Ho Man Tin, Hong Kong, China. Electronic address:

Bacteria detection and toxicity measurement are essential in many aspects. Becoming increasingly popular in recent years, paper-based analytical devices (PADs) have proven to be cost-effective, portable and eco-friendly with quantitative diagnostic results. In this work, by a straightforward soaking-drying method, a resazurin-deposited PAD has been developed for rapid bacteria detection and biotoxicity measurement. The colorimetric response on the PAD was generated from metabolic reduction of resazurin by Enterococcus faecalis, a facultative anaerobic bacterial strain. After recording and quantifying the colorimetric response with Hue value by a smartphone, the bioassay on PAD enables the detection of resazurin reduction kinetics difference among bacteria at various densities in 10 min. Thereby, the bioassay on PAD was applied to study the toxicity of two chlorophenols, i.e. pentachlorophenol (PCP) and 4-chlorophenol (4-CP), to E. faecalis. Compared to growth-based inhibition test, which takes 5 h, this assay shows higher efficiency, i.e. in 30 min, the biotoxicity difference between PCP and 4-CP can be identified.
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http://dx.doi.org/10.1016/j.talanta.2020.121966DOI Listing
April 2021

Correction to: Development of a highly sensitive fluorescence method for tartrazine determination in food matrices based on carbon dots.

Anal Bioanal Chem 2021 Mar 12;413(8):2275. Epub 2021 Feb 12.

College of Food Science and Engineering, Yangzhou University, Yangzhou, 225001, Jiangsu, China.

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http://dx.doi.org/10.1007/s00216-021-03217-7DOI Listing
March 2021

Safety and Efficacy of Bromodomain and Extra-Terminal Inhibitors for the Treatment of Hematological Malignancies and Solid Tumors: A Systematic Study of Clinical Trials.

Front Pharmacol 2020 26;11:621093. Epub 2021 Jan 26.

Laboratory for Stem Cell and Regenerative Medicine, Affiliated Hospital of Weifang Medical University, Weifang, China.

The upregulated expression of BET proteins is closely associated with the occurrence and development of hematological malignancies and solid tumors. Several BET inhibitors have been developed, and some have been in phase I/II of clinical trials. Here, the safety, efficacy, and pharmacodynamics of ten BET inhibitors currently in clinical trials were evaluated. We retrieved and reviewed published reports on the clinical trials of twelve BET inhibitors including AZD5153, ABBV-075, BMS-986158, CPI-0610, GSK525762, OTX-015, PLX51107, INCB054329, INCB057643, FT-1101, CC-90010, and ODM-207 for patients with hematological malignancies and solid tumors and summarized their published target genes. In the monotherapy of BET inhibitors, the most common and severe (grade ≥3) hematological adverse events (AEs) are thrombocytopenia, anemia, and neutropenia. The most common non-hematological syndromes are diarrhea, nausea, fatigue, dysgeusia, and decreased appetite, while the most severe AE is pneumonia. Additionally, of these BET inhibitors was between 0.5-6 h, but the range for varied significantly. According to published data, the rates of SD, PD, CR and PR were 27.4%, 37.6%, 3.5%, and 5.7%, respectively, which is not very satisfactory. In addition to BRD4, oncogene MYC is another common target gene of these BET inhibitors. Ninety-seven signaling pathways may be regulated by BET inhibitors. All BET inhibitors reviewed in our study exhibited exposure-dependent thrombocytopenia, which may limit their clinical application. Moreover, further efforts are necessary to explore the optimal dosing schemes and combinations to maximize the efficacy of BET inhibitors.
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http://dx.doi.org/10.3389/fphar.2020.621093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870522PMC
January 2021

Effective removal of ammonium nitrogen using titanate adsorbent: Capacity evaluation focusing on cation exchange.

Sci Total Environ 2021 Jun 27;771:144800. Epub 2021 Jan 27.

Department of Environmental Science and Engineering, State Key Laboratory of Multiphase Flow in Power Engineering, School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

Cation exchange is one of the dominant mechanisms in the adsorption of cationic ammonium nitrogen (NH) from water. In this study, we focus on the role of counter cations in cation exchange process of NH to enhance the adsorption capacity. Five amorphous titanates namely lithium titanate (LiT), sodium titanate (NaT), potassium titanate (KT), strontium titanate (SrT) and barium titanate (BaT) with different counter cation were facilely synthesized. The adsorption performance for NH by these samples is in the order of LiT> NaT > KT> > SrT > BaT. The maximum adsorption capacity of LiT calculated by Langmuir is as high as 50.31 mg·g. According to the experimental results and theoretical analysis, the electrostatic interaction between counter ions (cations in framework or eternal solution) and charged framework (fixed ions) is the main influence factor during cation exchange process in general. The cation valence and the hydrated ionic radius of the counter ions can inversely affect the ion exchange equilibrium and the affinity of counter ions to titanates. Therefore, a definition of a brief parameter, affinity coefficient K (relating to ion valence and distance between opposite charged ions), is introduced and used to explain the difference in adsorption performance of five titanates for NH. The conclusion about cation exchange and ions affinity may provide possible strategies for enhancement of cationic contaminant adsorption from water or wastewater.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144800DOI Listing
June 2021

Multi-Yolk-Shell MnO@Carbon Nanopomegranates with Internal Buffer Space as a Lithium Ion Battery Anode.

Langmuir 2021 Feb 3;37(6):2195-2204. Epub 2021 Feb 3.

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225002, P. R. China.

Multi-yolk-shell MnO@mesoporous carbon (MnO@m-carbon) nanopomegranates, featuring MnO nanoparticles within cavities of m-carbon with internal space between the MnO nanoparticle and a cavity carbon shell, were subtly constructed. Moreover, the buffer space was well controlled by means of regulating the size of the cavity in m-carbon or the content of MnO. The results of electrochemical measurements demonstrated that MnO(10)@m-carbon(22) nanopomegranates (MnO nanoparticle, 15 nm; cavity size, 22 nm) had the best cycling and rate performance for lithium ion storage. The pomegranate-like MnO@m-carbon nanostructures have shown several advantages for their excellent performance: the nanocavity in m-carbon can restrict the growth and agglomeration of MnO nanoparticles; the well-interconnected mesoporous carbon matrix provides a "highway" for electrons and lithium ion transport; the voids between the MnO nanoparticle and cavity shell can alleviate the volume expansion.
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http://dx.doi.org/10.1021/acs.langmuir.0c03523DOI Listing
February 2021

Biorepositories (biobanks) of human body fluids and materials as archives for tracing early infections of COVID-19.

Authors:
Shanshan He Jie Han

Environ Pollut 2021 Apr 18;274:116525. Epub 2021 Jan 18.

Department of Environmental Science and Engineering, Xi'an Jiaotong University, Xi'an, 710049, PR China. Electronic address:

Identifying the individuals and geographical regions witnessing early infections or outbreaks of SARS-CoV-2 and its variants is helpful for studying the early epidemiology or even the origin of the novel coronavirus. Here, we put forward a strategy that can potentially contribute to this goal. Human body fluids and biological materials collected before the COVID-19 pandemic may serve as archives for retrospective testing of early human infections before the recent outbreaks. These have been routinely donated, collected, and archived, creating biorepositories or "biobanks" for clinical or research purposes. SARS-CoV-2 genetic materials and its antibodies have been confirmed in various types of biological samples from COVID-19 patients, including blood, sperm, umbilical cord blood, lung, heart, kidney and so on, making these biological archives as candidates for detecting early COVID-19 infections. Unlike sewage-based epidemiology which only provides information on the geographical aspect, viruses identified in archived human biological samples provide direct links to individuals, from whom a wealth of personal information including their profession, hobbies and activities, travel history, and previous exposure to wildlife can all be retrieved. By analyzing the patterns and links in the behavior of those early infected individuals, it is possible to trace the origin of the virus, for instance, in certain wild animals or local environments.
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http://dx.doi.org/10.1016/j.envpol.2021.116525DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813484PMC
April 2021

Supramolecular brush polymers prepared from 1,3,4-oxadiazole and cyanobutoxy functionalised pillar[5]arene for detecting Cu.

Org Biomol Chem 2021 Feb;19(6):1287-1291

Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Energy), College of Chemistry, Nankai University, Tianjin 300071, China.

A supramolecular brush polymer Poly(P5-OXD) was constructed through the self-assembly of an A1/A2 disubstituted pillar[5]arene P5-OXD with a 1,3,4-oxadiazole unit and a cyanobutoxy group, exhibiting external stimuli responsiveness towards Cu2+ ions with an ON/OFF fluorescence signal output.
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http://dx.doi.org/10.1039/d0ob02587aDOI Listing
February 2021

N-, P-, and O-Tridoped Carbon Hollow Nanospheres with Openings in the Shell Surfaces: A Highly Efficient Electrocatalyst toward the ORR.

Langmuir 2021 Feb 27;37(5):2001-2010. Epub 2021 Jan 27.

School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002 Jiangsu, P.R. China.

Recently, carbon nanomaterials doped with nonmetallic atoms have been used as electrocatalysts involved in oxygen reduction reactions (ORRs) because of the lack of degradation and contamination problems caused by metal dissolution, low cost, sustainability, and multifunctionality. In this study, the metal-free N-, P-, O-tridoped carbon hollow nanospheres (N, P, O-Carbon) with openings in the shell surfaces have been developed, where poly(-phenylenediamine) hollow nanospheres with openings in the shell surfaces were chosen as a nitrogen-rich polymer, and then different phosphorus sources (such as NaHPO, HPO, and phytic acid (PA)) were introduced for heat treatment. When used as electrocatalysts, N, P, O-Carbon-PA showed the best ORR electroactivity with an onset potential ( of 0.98 V and the limit current density of 5.39 mA cm. The origin of high activity associated with heteroatom doping was elucidated by X-ray photoelectron spectroscopy and density functional theory. The results evidenced the high potential of N, P, O-Carbon as highly active nonmetal ORR electrocatalysts. It can be expected that the conclusions rendered herein will provide guidance for the reasonable design of other heteroatom-doped carbon for wider applications.
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http://dx.doi.org/10.1021/acs.langmuir.0c03620DOI Listing
February 2021

Risk factors for postoperative recurrent tricuspid regurgitation after concomitant tricuspid annuloplasty during left heart surgery and the association between tricuspid annular circumference and secondary tricuspid regurgitation.

BMC Cardiovasc Disord 2021 Jan 26;21(1):50. Epub 2021 Jan 26.

Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, No. 2 Anzhen Road, Chaoyang District, Beijing, 100029, China.

Background: To identify the association between tricuspid annular circumference and secondary tricuspid regurgitation and analyze the risk factors of recurrent tricuspid regurgitation after concomitant tricuspid annuloplasty during left heart surgery.

Methods: From October 2018 to June 2019, a total of 117 patients receiving concomitant tricuspid annuloplasty within left heart surgery were enrolled. Severity of tricuspid regurgitation was classified as 4 subtypes: normal, mild, moderate and severe. Perioperative data and mid-term outcome were collected. Tricuspid annular circumference (TAC) was measured under cardiac arrest during surgery procedure by cardioplegia. Optimal TAC and TAC index (TAC/body surface area, BSA) cutoffs of significant tricuspid annulus dilatation (moderate and severe) were obtained. Univariable and multivariable logistic regression analyses were performed to identify the risk factors of postoperative recurrent tricuspid regurgitation. The follow up period is 13-19 months (mean 15.5 ± 3.2 months).

Results: There was 1 patient was excluded who died after surgery. A total of 116 patients receiving tricuspid annuloplasty were included. Optimal cutoffs of significant tricuspid annulus dilatation were recommended (TAC 11.45 cm, Sensitivity 82.89%, Specificity 73.68%, AUC 0.915; TAC index 7.09 cm/m, Sensitivity 73.68%, Specificity 85%, AUC 0.825, respectively). Based on findings of multivariable logistic regression, it has been showed that TAC index and postoperative atrial fibrillation were the independent risk factors of recurrent regurgitation after surgery. Optimal TAC index cutoff to predict recurrent tricuspid regurgitation was 7.86 cm/m CONCLUSIONS: The severity of secondary tricuspid regurgitation is associated with the tricuspid annular circumference. The cut-offs of significant tricuspid regurgitation (more than moderate) were TAC 11.45 cm and TAC index 7.09 cm/m, respectively. Clinically, concomitant tricuspid annuloplasty is relative safe and effective. TAC index ≥ 7.86 cm/m and postoperative atrial fibrillation are the risk factors of recurrent significant tricuspid regurgitation after concomitant tricuspid annuloplasty during left heart surgery.
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http://dx.doi.org/10.1186/s12872-021-01870-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7836580PMC
January 2021

How human thermal plume influences near-human transport of respiratory droplets and airborne particles: a review.

Environ Chem Lett 2021 Jan 21:1-12. Epub 2021 Jan 21.

Department of Environmental Science and Engineering, Xi'an Jiaotong University, Xi'an, 710049 China.

With mounting evidence and notable cases of large clustered infections, airborne transmission via droplets and particles has been recently acknowledged as an effective mode of transmission for COVID-19. How droplets and aerosol particles disperse are being transported into the human breathing zone-the last few inches for airborne transmission to effectuate-remains a key question which has been widely overlooked. Human thermal plume refers to the constantly rising airflows around the boundary layer of human body due to persisting temperature gradients between the body surfaces and the ambient air. Ample evidence indicated that the thermal plume controls the dispersion and transport of aerosols in the human microenvironment. Given that in calm indoor environments most air inhaled by human comes from the boundary layer where thermal plume flows through constantly, the role of thermal plume needs to be scrutinized to predict the diffusion of droplets, aerosols and other airborne carriers of the novel coronavirus around the human body for prioritizing infection control strategies. Here, we assessed the potential influences of the thermal plume on the transmission of COVID-19 and other airborne pathogens by reviewing the most pertinent evidence and analyzing key variables in the formation of thermal plume in indoor environments, e.g., ambient temperature, human posture and type of clothing. Our reviewed evidence and data indicate that the human thermal plume should facilitate the airborne transmission of COVID-19 in enclosed spaces by elevating small droplets and airborne particles into the breathing zone from lower regions and ascending respiratory droplets from the sources into the upper atmosphere. By drawing attention to aerosol transport dynamics in the human microenvironment, these insights may be useful for understanding COVID-19 transmission in enclosed spaces, especially those intended for public use.
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http://dx.doi.org/10.1007/s10311-020-01178-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817963PMC
January 2021

Development of a highly sensitive fluorescence method for tartrazine determination in food matrices based on carbon dots.

Anal Bioanal Chem 2021 Feb 19;413(5):1485-1492. Epub 2021 Jan 19.

College of Food Science and Engineering, Yangzhou University, Yangzhou, 225001, Jiangsu, China.

In this work, an ultrasensitive sensing system based on fluorescent carbon dots (CDs) was developed for the tartrazine (Tar) determination. The CDs were prepared via a simple one-pot hydrothermal method with m-phenylenediamine as the only precursor. The physical and chemical properties were in detail characterized by transmission electron microscopy (TEM), MALDI-TOF MS, UV-vis absorption and photoluminescence (PL) spectroscopy, elemental analysis, and Fourier transform infrared spectroscopy (FTIR). Upon exposure to Tar, the fluorescence of CDs was efficiently quenched via the dynamic interaction between CDs and Tar as well as the inner filter effect (IFE). With this information, the CDs were proposed as a fluorescence probe for Tar detection. It was found that CDs had high sensitivity and selectivity for Tar sensing, and the linear relationship was observed in the range of 0.01-25.0 μM with the corresponding detection limit (3σ/k) of 12.4 nM, which is much more sensitive than any of the existed CD-based sensing platform. The investigated sensing system was finally utilized for Tar sensing in various food matrices with a high degree of accuracy. The spiked recoveries were in a range of 96.4-105.2%, and the relative standard deviations (RSDs) were lower than 4.13%. This work highlights the great application prospects of CDs for Tar sensing in a rapid, simple, and sensitive way.
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http://dx.doi.org/10.1007/s00216-020-03118-1DOI Listing
February 2021

Role of indoor aerosols for COVID-19 viral transmission: a review.

Environ Chem Lett 2021 Jan 13:1-18. Epub 2021 Jan 13.

Department of Environmental Science and Engineering, Xi'an Jiaotong University, Xi'an, 710049 People's Republic of China.

The relationship between outdoor atmospheric pollution by particulate matter and the morbidity and mortality of coronavirus disease 2019 (COVID-19) infections was recently disclosed, yet the role of indoor aerosols is poorly known . Since people spend most of their time indoor, indoor aerosols are closer to human occupants than outdoors, thus favoring airborne transmission of COVID-19. Therefore, here we review the characteristics of aerosol particles emitted from indoor sources, and how exposure to particles affects human respiratory infections and transport of airborne pathogens. We found that tobacco smoking, cooking, vacuum cleaning, laser printing, burning candles, mosquito coils and incenses generate large quantities of particles, mostly in the ultrafine range below 100 nm. These tiny particles stay airborne, are deposited in the deeper regions of human airways and are difficult to be removed by the respiratory system. As a consequence, adverse effects can be induced by inhaled aerosol particles via oxidative stress and inflammation. Early epidemiological evidence and animal studies have revealed the adverse effects of particle exposure in respiratory infections. In particular, inhaled particles can impair human respiratory systems and immune functions, and induce the upregulation of angiotensin-converting enzyme 2, thus inducing higher vulnerability to COVID-19 infection. Moreover, co-production of inflammation mediators by COVID-19 infection and particle exposure magnifies the cytokine storm and aggravates symptoms in patients. We also discuss the role of indoor aerosol particles as virus carriers. Although many hypotheses were proposed, there is still few knowledge on interactions between aerosol articles and virus-laden droplets or droplet nuclei.
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http://dx.doi.org/10.1007/s10311-020-01174-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7805572PMC
January 2021

How mangrove plants affect microplastic distribution in sediments of coastal wetlands: Case study in Shenzhen Bay, South China.

Sci Total Environ 2021 May 30;767:144695. Epub 2020 Dec 30.

College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China; Futian-CityU Mangrove R&D Centre, City University of Hong Kong Shenzhen Research Institute, Shenzhen, China. Electronic address:

Microplastic pollution is common in marine and coastal ecosystems, especially in mangrove wetlands. However, factors affecting the distribution of microplastics, such as plants, have not been sufficiently studied. We investigated the effect of different plant species on the distribution of sediment microplastics in two Nature Reserves in South China, viz. Futian Mangrove and Mai Po Mangrove. In Futian Mangrove, the abundance of total microplastics among three monospecific mangrove stands dominated by Sonneratia caseolaris, Kandelia obovata, and Sonneratia apetala was similar. The abundance of microplastics in the mudflat was similar to that in the forest interior, except for the fact that more fiber was found in the mudflat than in the interior of Sonneratia apetala. This suggested that the dense pneumatophores at the fringe prevented fibers from entering the mangrove forest. The significant positive dependence (p < 0.05) between the density of Sonneratia pneumatophores and the abundance of fibers highlighted the importance of pneumatophores. The abundance of total microplastics, predominantly in the form of fibers, in sediments at the forest fringe (2835 ± 713 items/kg d.w. and 2070 ± 224 items/kg d.w. in Futian and Mai Po, respectively) was higher than that in the forest interior and mudflat. There was no difference between the two latter locations in both mangroves, which demonstrated the significance of the fringe effect. This paper reports for the first time that the spatial distribution of microplastics in mangrove sediments was affected by plant species, which provides useful information for environmental processes of microplastics in coastal wetlands.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144695DOI Listing
May 2021