Publications by authors named "Jie Fu"

489 Publications

Antimicrobial Peptides in Gut Health: A Review.

Front Nutr 2021 30;8:751010. Epub 2021 Sep 30.

Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou, China.

Animal antimicrobial peptides (AMPs), known as broad-spectrum and high-efficiency antibacterial activity, are important effector molecules in innate immune system. AMPs not only have antimicrobial, antiviral and antitumor effects but also exhibit important effects , such as anti-inflammatory response, recruiting immune cells, promoting epithelial damage repair, and promoting phagocytosis of bacteria. However, research on the application of AMPs is incomplete and controversial. This review mainly introduces the classification of AMPs, biological functions, as well as the mechanisms of action, expression rules, and nutrition regulation from three perspectives, aiming to provide important information for the application of AMPs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnut.2021.751010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8514777PMC
September 2021

Encapsulating Fe O Nanotubes into Carbon-Coated Co S Nanocages Derived from a MOFs-Directed Strategy for Efficient Oxygen Evolution Reactions and Li-Ions Storage.

Small 2021 Oct 15:e2103178. Epub 2021 Oct 15.

School of Environmental and Chemical Engineering, Shanghai University, 99 Shangda Road, Shanghai, 200444, P. R. China.

The development of high-efficiency, robust, and available electrode materials for oxygen evolution reaction (OER) and lithium-ion batteries (LIBs) is critical for clean and sustainable energy system but remains challenging. Herein, a unique yolk-shell structure of Fe O [email protected] Co S [email protected] is rationally prepared. In a prearranged sequence, the fabrication of Fe O nanotubes is followed by coating of zeolitic imidazolate framework (ZIF-67) layer, chemical etching of ZIF-67 by thioacetamide, and eventual annealing treatment. Benefiting from the hollow structures of Fe O nanotubes and Co S nanocages, the conductivity of carbon coating and the synergy effects between different components, the titled sample possesses abundant accessible active sites, favorable electron transfer rate, and exceptional reaction kinetics in the electrocatalysis. As a result, excellent electrocatalytic activity for alkaline OER is achieved, which delivers a low overpotential of 205 mV at the current density of 10 mA cm along with the Tafel slope of 55 mV dec . Moreover, this material exhibits excellent high-rate capability and excellent cycle life when employed as anode material of LIBs. This work provides a novel approach for the design and the construction of multifunctional electrode materials for energy conversion and storage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202103178DOI Listing
October 2021

A self-healing catalyst for electrocatalytic and photoelectrochemical oxygen evolution in highly alkaline conditions.

Nat Commun 2021 Oct 13;12(1):5980. Epub 2021 Oct 13.

Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, 610054, Chengdu, China.

While self-healing is considered a promising strategy to achieve long-term stability for oxygen evolution reaction (OER) catalysts, this strategy remains a challenge for OER catalysts working in highly alkaline conditions. The self-healing of the OER-active nickel iron layered double hydroxides (NiFe-LDH) has not been successful due to irreversible leaching of Fe catalytic centers. Here, we investigate the introduction of cobalt (Co) into the NiFe-LDH as a promoter for in situ Fe redeposition. An active borate-intercalated NiCoFe-LDH catalyst is synthesized using electrodeposition and shows no degradation after OER tests at 10 mA cm at pH 14 for 1000 h, demonstrating its self-healing ability under harsh OER conditions. Importantly, the presence of both ferrous ions and borate ions in the electrolyte is found to be crucial to the catalyst's self-healing. Furthermore, the implementation of this catalyst in photoelectrochemical devices is demonstrated with an integrated silicon photoanode. The self-healing mechanism leads to a self-limiting catalyst thickness, which is ideal for integration with photoelectrodes since redeposition is not accompanied by increased parasitic light absorption.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-26281-0DOI Listing
October 2021

A case study on TBM cutterhead temperature monitoring and mud cake formation discrimination method.

Sci Rep 2021 Oct 7;11(1):19983. Epub 2021 Oct 7.

College of Mechanical and Electrical Engineering, Central South University, Changsha, 410083, Hunan, People's Republic of China.

The mud cake is easily formed during the tunnel boring machine (TBM) excavation in clay soils or rocks containing clay minerals. Mud cake will lead to soil disturbance of tunnel face, clogging cutterhead and even affect the construction efficiency and personnel safety. In this study, a discrimination method of mud cake formation based on cutterhead temperature was proposed. An online monitoring system was designed and installed on the slurry balance TBM. The results show that: (a) the cutterhead temperature data can be reliably detected and transmitted by the system; (b) in a tunneling cycle, the temperature at some positions of the cutterhead will increase first and then decrease; (c) during the field test, the temperature variation is around 2.5 °C under the normal condition, but the temperature variation will increase more than 50 °C due to the mud cake or geological change; (d) compared with the cooling rate, mud cake formation can be accurately discriminated.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-99439-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497619PMC
October 2021

Occurrence, profiles, and ecotoxicity of poly- and perfluoroalkyl substances and their alternatives in global apex predators: A critical review.

J Environ Sci (China) 2021 Nov 4;109:219-236. Epub 2021 May 4.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China; School of Environment, Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences, Hangzhou 310000, China; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances, Institute of Environment and Health, Jianghan University, Wuhan 430010, China. Electronic address:

Certain poly- and perfluoroalkyl substances (PFASs) exhibit significant bioaccumulation/biomagnification behaviors in ecosystems. PFASs, such as perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), perfluorohexanesulfonic acid (PFHxS) and related precursors, have elicited attention from both public and national regulatory agencies, which has resulted in worldwide restrictions on their production and use. Apex predators occupy the top trophic positions in ecosystems and are most affected by the biomagnification behavior of PFASs. Meanwhile, the long lifespans of apex predators also lead to the high body burden of PFASs. The high body burden of PFASs might be linked to adverse health effects and even pose a potential threat to their reproduction. As seen in previous reviews of PFASs, knowledge is lacking between the current stage of the PFAS body burden and related effects in apex predators. This review summarized PFAS occurrence in global apex predators, including information on the geographic distribution, levels, profiles, and tissue distribution, and discussed the trophic transfer and ecotoxicity of PFASs. In the case where legacy PFASs were restricted under international convention, the occurrence of novel PFASs, such as 6:2 chlorinated polyfluorinated ether sulfonate (6:2 Cl-PFESA) and perfluoroethylcyclohexane sulfonate (PFECHS), in apex predators arose as an emerging issue. Future studies should develop an effective analytical method and focus on the toxicity and trophic transfer behavior of novel PFASs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jes.2021.03.036DOI Listing
November 2021

The Role of m6A Ribonucleic Acid Modification in the Occurrence of Atherosclerosis.

Front Genet 2021 16;12:733871. Epub 2021 Sep 16.

School of Basic Medicine Sciences, Weifang Medical University, Weifang, China.

The N6-methyladenosine (m6A) modification is the most abundant epitranscriptomic modification in eukaryotic messenger RNA (mRNA). The m6A modification process is jointly regulated by various enzymes and proteins, such as methyltransferases, demethylases and related m6A-binding proteins. The process is dynamic and reversible, and it plays an essential role in mRNA metabolism and various biological activities. Recently, an increasing number of researchers have confirmed that the onset and development of many diseases are closely associated with the molecular biological mechanism of m6A RNA methylation. This study focuses on the relationship between m6A RNA modification and atherosclerosis (AS). It thoroughly summarizes the mechanisms and processes of m6A RNA modification in AS-related cells and the relationships between m6A RNA modification and AS risk factors, and it provides a reference for exploring new targets for the early diagnosis and treatment of AS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2021.733871DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481608PMC
September 2021

Comprehensive analysis of abnormal expression, prognostic value and oncogenic role of the hub gene FN1 in pancreatic ductal adenocarcinoma bioinformatic analysis and experiments.

PeerJ 2021 6;9:e12141. Epub 2021 Sep 6.

Department of General Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.

Background: Pancreatic ductal adenocarcinoma (PDAC) is one of the most commonly diagnosed cancers with a poor prognosis worldwide. Although the treatment of PDAC has made great progress in recent years, the therapeutic effects are still unsatisfactory. Methods. In this study, we identified differentially expressed genes (DEGs) between PDAC and normal pancreatic tissues based on four Gene Expression Omnibus (GEO) datasets (GSE15471, GSE16515, GSE28735 and GSE71729). A protein-protein interaction (PPI) network was established to evaluate the relationship between the DEGs and to screen hub genes. The expression levels of the hub genes were further validated through the Gene Expression Profiling Interactive Analysis (GEPIA), ONCOMINE and Human Protein Atlas (HPA) databases, as well as the validation GEO dataset GSE62452. Additionally, the prognostic values of the hub genes were evaluated by Kaplan-Meier plotter and the validation GEO dataset GSE62452. Finally, the mechanistic roles of the most remarkable hub genes in PDAC were examined through experiments.

Results: We identified the following nine hub genes by performing an integrated bioinformatics analysis: COL1A1, COL1A2, FN1, ITGA2, KRT19, LCN2, MMP9, MUC1 and VCAN. All of the hub genes were significantly upregulated in PDAC tissues compared with normal pancreatic tissues. Two hub genes (FN1 and ITGA2) were associated with poor overall survival (OS) rates in PDAC patients. Finally, experiments indicated that FN1 plays vital roles in PDAC cell proliferation, colony formation, apoptosis and the cell cycle.

Conclusions: In summary, we identified two hub genes that are associated with the expression and prognosis of PDAC. The oncogenic role of FN1 in PDAC was first illustrated by performing an integrated bioinformatic analysis and experiments. Our results provide a fundamental contribution for further research aimed finding novel therapeutic targets for overcoming PDAC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.12141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428264PMC
September 2021

Molecular Analyses of Petroleum Hydrocarbon Change and Transformation during Petroleum Weathering by Multiple Techniques.

ACS Omega 2021 Sep 31;6(36):23222-23232. Epub 2021 Aug 31.

School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

Various analytical techniques are used to study the weathering process of four crude oils, i.e., Iranian light crude oil, Daqing crude oil, Shengli crude oil, and Tahe crude oil. The molecular composition and structural information of -alkanes, polycyclic aromatic hydrocarbons (PAHs), and heteroatom compounds were characterized by gas chromatography-flame ionization detector (GC-FID), gas chromatography-mass spectrometry (GC-MS), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), respectively. The results showed that the weathering loss of -alkanes was related to the molecular weight, and the low-molecular-weight (LMW) -alkanes were more volatile. The loss degree of LMW naphthalene and alkylation homologues in PAHs was also higher. With the increase in the alkylation degree, the weathering resistance ability of PAHs was enhanced. In the negative-ion ESI FT-ICR MS mode, a total of 16 classes of compounds were detected for neutral nitrogen compounds and acidic compounds in the four crude oils. With the increase in weathering time, the relative abundances of NO, NO2, and O3S compounds gradually increased. In particular, the NO and NO2 compounds with different condensation degrees increased significantly. These results indicated that in addition to the volatilization of hydrocarbon compounds, nitrogen compounds were also oxidized to a certain extent during the weathering process. The provided information would enrich the understanding of the short-term weathering process of petroleum hydrocarbons.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.1c02846DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8444223PMC
September 2021

Up-regulation of GLP-1R improved the dysfunction of late EPCs under hyperglycemia by regulating SIRT1 expression.

Mol Cell Endocrinol 2021 Sep 10;538:111455. Epub 2021 Sep 10.

Department of Cardiology, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, Hubei, China. Electronic address:

The dysfunction of endothelial progenitor cells (EPCs) is closely associated with diabetic vascular complications. Both glucagonlike peptide-1 receptor (GLP-1R) and silent information regulator 1 (SIRT1) can control systemic glucose homeostasis and protect endothelial cells against hyperglycemia-induced oxidative stress. In this study, we mainly assessed the role played by SIRT1 and GLP-1R and their relationship in regulating the function of late EPCs under hyperglycemia stimulation. Human peripheral blood mononuclear cells (PBMCs) were cultured in EGM-2 medium and induced to differentiate into EPCs and 25 mM glucose was used to stimulate EPCs to obtain a hyperglycemia condition. Subsequently, the expression and location of GLP-1R and SIRT1 in EPCs were detected. After GLP-1R or SIRT1 knockdown, or the treatment by GLP-1R agonist and/or SIRT1 agonist/inhibitor, the effects of SIRT1 and GLP-1R and their relationship in regulating the function of late EPCs under hyperglycemia stimulation was studied by detecting the apoptosis, migration, adhesion and angiogenicity abilities of EPCs. Results demonstrated that, in high-glucose stimulated EPCs, the expression of GLP-1R and SIRT1 was down-regulated. The knockdown of either GLP-1R or SIRT1 could increase EPCs apoptosis and weaken the migration, adhesion and angiogenicity abilities of EPCs. In addition, the improvement effects of Exendin-4 or GLP-1R over-expression on EPCs dysfunction could be weakened to some degree under SIRT1 knockdown. In conclusion, both GLP-1R and SIRT1 expression played important roles in regulating EPCs dysfunction under hyperglycemia and the up-regulation of GLP-1R improved the dysfunction of late EPCs by regulating SIRT1 expression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.mce.2021.111455DOI Listing
September 2021

Microwave pyrolysis of oily sludge under different control modes.

J Hazard Mater 2021 08 15;416:125887. Epub 2021 Apr 15.

Tianjin Chemical Research & Design Institute, China National Offshore Oil Corporation, Tianjin 300131, China.

The effects of temperature and power on product distribution and characteristics of oily sludge (OS) pyrolysis were investigated in a microwave reactor. The maximum oil yield was 72.55 wt% at 550 °C and 71.47 wt% at 800 W, respectively. X-ray photoelectron spectroscopy (XPS) indicated that C-C and C-O were the main forms of carbon in OS char (OC). The sulfur (S) content in OC increased as the temperature/power rose, implying that S might exist in the form of inorganics or OC had S retention ability. In temperature control mode, the changes of functional groups on OC surface were more sensitive. The maximum hydrocarbon content in oil was 14.56% at 350 °C and 13.40% at 900 W, respectively. The contents of oxygenated compounds and heterocycles in oil from temperature control mode were higher. The CO yield increased with increasing temperature/power, reaching the maximum of 9.60 wt% at 650 °C and 7.75 wt% at 900 W, respectively. Compared with power control mode, it seemed that more heavy metals (HMs) were retained in OC in temperature control mode. The E of HMs were at the clean level and RI indicated the HMs in OC had a low environmental risk.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2021.125887DOI Listing
August 2021

Rosiglitazone Alleviates Mechanical Allodynia of Rats with Bone Cancer Pain through the Activation of PPAR- to Inhibit the NF-B/NLRP3 Inflammatory Axis in Spinal Cord Neurons.

PPAR Res 2021 25;2021:6086265. Epub 2021 Aug 25.

The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University/The Second School of Medicine, Wenzhou Medical University, Wenzhou, China.

Bone cancer pain (BCP) is a serious clinical problem that affects the quality of life of cancer patients. However, the current treatment methods for this condition are still unsatisfactory. This study investigated whether intrathecal injection of rosiglitazone modulates the noxious behaviors associated with BCP, and the possible mechanisms related to this effect were explored. We found that rosiglitazone treatment relieved bone cancer-induced mechanical hyperalgesia in a dose-dependent manner, promoted the expression of peroxisome proliferator-activated receptor- (PPAR-) in spinal cord neurons, and inhibited the activation of the nuclear factor-kappa B (NF-B)/nod-like receptor protein 3 (NLRP3) inflammatory axis induced by BCP. However, concurrent administration of the PPAR- antagonist GW9662 reversed these effects. The results show that rosiglitazone inhibits the NF-B/NLRP3 inflammation axis by activating PPAR- in spinal neurons, thereby alleviating BCP. Therefore, the PPAR-/NF-B/NLRP3 signaling pathway may be a potential target for the treatment of BCP in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/6086265DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413064PMC
August 2021

Electroacupuncture Attenuates Cancer-Induced Bone Pain via NF-κB/CXCL12 Signaling in Midbrain Periaqueductal Gray.

ACS Chem Neurosci 2021 09 30;12(18):3323-3334. Epub 2021 Aug 30.

Department of Anesthesiology and Pain Research Center, The Affiliated Hospital of Jiaxing University, 1882 Zhonghuan South Road, Jiaxing 314001, China.

Electroacupuncture (EA) is effective in various chronic pains. NF-κB and CXCL12 modulate the formation of chronic pain. Herein, we hypothesized that EA alleviates cancer-induced bone pain (CIBP) through NF-κB/CXCL12 axis in midbrain periaqueductal gray (PAG), which participates in "top-down" pain modulatory circuits. In order to filter the optimum EA frequency for CIBP treatment, 2, 100, or 2/100 Hz EA was set up. In addition, ipsilateral, contralateral, and bilateral EA groups were established to affirm the optimal EA scheme. Bilateral 2/100 Hz EA was considered as the optimal therapeutic scheme and was applied in a subsequent experiment. Western blotting along with immunofluorescence illustrated that CIBP induces a rapid and substantial increase in CXCL12 protein level and NF-κB phosphorylation in vlPAG from day 6 to day 12. Anti-CXCL12 neutralizing antibody and pAAV-U6-shRNA(CXCL12)-CMV-EGFP-WPRE in vlPAG remarkably improved the mechanical pain threshold of the hind paw in CIBP model relative to the control. EA inhibited the upregulation of pNF-κB and CXCL12 in vlPAG of CIBP. The recombinant CXCL12 and pAAV-CMV-CXCL12-EF1a-EGFP-3Xflag-WPRE reversed the abirritation of EA in the CIBP rat model. NF-κB phosphorylation mediated-CXCL12 expression contributed to CIBP allodynia, whereas EA suppressed NF-κB phosphorylation in CIBP. According to the above evidence, we conclude that bilateral 2/100 Hz EA is an optimal therapeutic scheme for CIBP. The abirritation mechanism of EA might reduce the expression of CXCL12 by inhibiting the activation of NF-κB, which might lead to the restraint of descending facilitation of CIBP.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acschemneuro.1c00224DOI Listing
September 2021

An Automatic Deep Learning-Based Workflow for Glioblastoma Survival Prediction Using Preoperative Multimodal MR Images: A Feasibility Study.

Adv Radiat Oncol 2021 Sep-Oct;6(5):100746. Epub 2021 Jul 1.

Department of Radiation Oncology, Cedars-Sinai Medical Center, Los Angeles, California.

Purpose: Most radiomic studies use the features extracted from the manually drawn tumor contours for classification or survival prediction. However, large interobserver segmentation variations lead to inconsistent features and hence introduce more challenges in constructing robust prediction models. Here, we proposed an automatic workflow for glioblastoma (GBM) survival prediction based on multimodal magnetic resonance (MR) images.

Methods And Materials: Two hundred eighty-five patients with glioma (210 GBM, 75 low-grade glioma) were included. One hundred sixty-three of the patients with GBM had overall survival data. Every patient had 4 preoperative MR images and manually drawn tumor contours. A 3-dimensional convolutional neural network, VGG-Seg, was trained and validated using 122 patients with glioma for automatic GBM segmentation. The trained VGG-Seg was applied to the remaining 163 patients with GBM to generate their autosegmented tumor contours. The handcrafted and deep learning (DL)-based radiomic features were extracted from the autosegmented contours using explicitly designed algorithms and a pretrained convolutional neural network, respectively. One hundred sixty-three patients with GBM were randomly split into training (n = 122) and testing (n = 41) sets for survival analysis. Cox regression models were trained to construct the handcrafted and DL-based signatures. The prognostic powers of the 2 signatures were evaluated and compared.

Results: The VGG-Seg achieved a mean Dice coefficient of 0.86 across 163 patients with GBM for GBM segmentation. The handcrafted signature achieved a C-index of 0.64 (95% confidence interval, 0.55-0.73), whereas the DL-based signature achieved a C-index of 0.67 (95% confidence interval, 0.57-0.77). Unlike the handcrafted signature, the DL-based signature successfully stratified testing patients into 2 prognostically distinct groups.

Conclusions: The VGG-Seg generated accurate GBM contours from 4 MR images. The DL-based signature achieved a numerically higher C-index than the handcrafted signature and significant patient stratification. The proposed automatic workflow demonstrated the potential of improving patient stratification and survival prediction in patients with GBM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.adro.2021.100746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377554PMC
July 2021

Encapsulation of CuO nanoparticles within silicalite-1 as a regenerative catalyst for transfer hydrogenation of furfural.

iScience 2021 Aug 17;24(8):102884. Epub 2021 Jul 17.

Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.

Catalytic transfer hydrogenation (CTH) of biomass-derived furfural (FAL) to furfuryl alcohol is recognized as one of the most versatile techniques for biomass valorization. However, the irreversible sintering of metal sites under the high-temperature reaction or during the coke removal regeneration process poses a serious concern. Herein, we present a silicalite-1-confined ultrasmall CuO structure ([email protected]) and then compared its catalytic efficiency against conventional surface-supported CuO structure (CuO/silicalite-1) toward CTF of FAL with alcohols. Characterization results revealed that CuO nanoparticles encapsulated within the silicalite-1 matrix are ∼1.3 nm in size in [email protected], exhibiting better dispersion as compared to that in the CuO/silicalite-1. The [email protected], as a result, exhibited nearly 100-fold higher Cu-mass-based activity than the CuO/silicalite-1 counterpart. More importantly, the activity of the [email protected] catalyst can be regenerated via facile calcination to remove the surface-bound carbon deposits, unlike the CuO/silicalite-1 that suffered severe deactivation after use and cannot be effectively regenerated.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.isci.2021.102884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355926PMC
August 2021

Sulforaphane alleviates hyperalgesia and enhances analgesic potency of morphine in rats with cancer-induced bone pain.

Eur J Pharmacol 2021 Oct 8;909:174412. Epub 2021 Aug 8.

Department of Anesthesiology and Pain Research Center, Affiliated Hospital of Jiaxing University & The First Hospital of Jiaxing, Jiaxing, China. Electronic address:

Due to the efficacy and tolerability of the available drugs, the current treatment for cancer-induced bone pain (CIBP) is not considered ideal, and new drugs are required for better treatment results. This study investigated whether intrathecal injection of sulforaphane (SFN) can modulates the noxious behavior associated with CIBP and enhances the analgesic effects of morphine and the possible mechanisms related to these effects were investigated. Walker256 breast cancer cells were injected into the bone marrow cavity of rats to establish the CIBP model. When CIBP rats began to exhibit painful behavior (CIBP 6 days), SFN was injected intrathecally for 7 days. The results showed that SFN alleviated the painful behavioral hypersensitivity caused by cancer, accompanied by nuclear factor, erythroid 2 like 2 (Nrf2), Haem oxygenase 1 (HO-1) activation, nuclear factor kappa B (NF-κB) inhibition and inflammation-related factors (tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-β), interleukin-6 (IL-6), and inducible nitric oxide synthase (iNOS) reduction. In addition, SFN treatment inhibited the proliferation of Walker 256 cells in a dose-dependent manner, promoted mu-opioid receptor (MOR) expression in SH-SY5Y cells and enhanced the antihyperalgesic effects of morphine on CIBP rats by restoring the downregulation of MOR expression in the spinal cord. Interestingly, the antihyperalgesic effects of SFN were partially blocked by opioid receptor antagonists. This study showed that SFN combined with morphine might be a new way to treat CIBP.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejphar.2021.174412DOI Listing
October 2021

Effects of Sodium Valproate Monotherapy on Blood Liver Enzyme Levels in Patients with Epilepsy: A Meta-Analysis.

Horm Metab Res 2021 Jul 19;53(7):425-434. Epub 2021 Jul 19.

Department of Neurosurgery, the Affiliated Hospital of Southwest Medical University, Luzhou, China.

We conducted this meta-analysis to assess the effects of sodium valproate (VPA) monotherapy on blood liver enzymes in patients with epilepsy. PubMed, Web of Science, EBSCO, Cochrane Library, Wanfang, China national knowledge infrastructure databases were searched. Nine studies were included. Results showed: (1) The overall SMD for blood AST, ALT, and GGT levels of VPA monotherapy group versus control group were 0.70 (95% CI=0.31 to 1.09, Z=3.52, p=0.0004), 0.47 (95% CI=- 0.01 to 0.95, Z=1.91, p=0.06), 0.44 (95% CI=0.29 to 0.60, Z=5.55, p<0.00001), respectively. (2) In subgroup meta-analysis, increased blood AST and GGT levels were observed in epileptic minors (AST: total SMD=0.85, 95% CI=0.40 to 1.30, Z=3.69, p=0.0002; GGT: total SMD=0.46, 95% CI=0.29 to 0.63, Z=5.25, p<0.00001). Elevated blood ALT level was observed in Asian patients receiving VPA monotherapy (total SMD=0.70, 95% CI=0.51 to 0.90, Z=7.01, p<0.00001), and the early stage of VPA monotherapy (total SMD=0.93, 95% CI=0.57 to 1.29, Z=5.09, p<0.00001). Overall, our results indicated that blood AST and GGT were significantly increased in epileptic minors receiving VPA monotherapy. The elevation of blood ALT was observed in Asian patients and the early stage of VPA monotherapy. However, due to the small number of included studies, our results should be considered with caution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1055/a-1517-6550DOI Listing
July 2021

Petroleum oil and products recovery from oily sludge: Characterization and analysis of pyrolysis products.

Environ Res 2021 Jul 16;202:111675. Epub 2021 Jul 16.

Beijing Advanced Innovation Center for Soft Matter Science and Engineering, State Key Laboratory of Organic-Inorganic Composites, College of Chemical Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.

Oily sludge (OS) has attracted special interest because of its hazardous nature and high potential as an energy resource. This study investigated the oil recovery from OS by thermal cracking and catalytic pyrolysis. The oil yield increased when the temperature exceeded 450 °C and reached a maximum (76.84 wt%) at 750 °C. Catalysts significantly improved the quality of oil produced during catalytic pyrolysis. Aromatic hydrocarbons were dominant (10.01-52.69%) in pyrolysis oil (PO) from OS catalytic pyrolysis, and the catalysts significantly reduced the presence of oxygen heterocycles. In addition, KOH and CaO reduced the I (D-band peak intensity)/I (G-band peak intensity) of OS char (OC) and increased the degree of graphitization. Owing to its higher iodine adsorption value and methylene blue (MB) adsorption value, OC exhibits potential as an adsorbent. The environmental assessment and potential applications of OC, along with possible reaction mechanisms and kinetic characteristics, are also discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envres.2021.111675DOI Listing
July 2021

Microwave-assisted pyrolysis of oily sludge from offshore oilfield for recovery of high-quality products.

J Hazard Mater 2021 Oct 3;420:126578. Epub 2021 Jul 3.

Tianjin Chemical Research & Design Institute, China National Offshore Oil Corporation, Tianjin 300131, China.

Microwave pyrolysis of oily sludge (OS) was investigated in this study. In this case, the highest oil yield (85.93 wt%) was achieved at 500 °C. The molar ratio of H/C was lower for OS char (OC) at higher pyrolysis temperatures, indicating good stability of OC owing to high degree of carbonization and aromaticity. Then, iodine adsorption value of OC reached maximum (531.2 mg/g) at 750 °C. While methylene blue (MB) uptake slightly increased with temperature and reached maximum (384.08 mg/g) at 850 °C. In order to improve the quality of pyrolysis products, different catalysts were employed in OS pyrolysis. The maximum content (64.31%) of aromatic hydrocarbon was found in PO500-10β. In addition, β-zeolite also reduced oxygenates content in oil, beneficial for stability of oil products. The gas products from catalytic pyrolysis were abundant in CO and CH, and KOH achieved the highest CO (5.9 wt%), CH (16.9 wt%) and H (2.4 wt%) yields. Finally, a reaction mechanism pathway for OS pyrolysis was proposed to show the production routes of gas, liquid, and solid products.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2021.126578DOI Listing
October 2021

Long-Range Transport, Trophic Transfer, and Ecological Risks of Organophosphate Esters in Remote Areas.

Environ Sci Technol 2021 08 15;55(15):10192-10209. Epub 2021 Jul 15.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

Organophosphate esters (OPEs) have been a focus in the field of environmental science due to their large volume production, wide range of applications, ubiquitous occurrence, potential bioaccumulation, and worrisome ecological and health risks. Varied physicochemical properties among OPE analogues represent an outstanding scientific challenge in studying the environmental fate of OPEs in recent years. There is an increasing number of studies focusing on the long-range transport, trophic transfer, and ecological risks of OPEs. Therefore, it is necessary to conclude the OPE pollution status on a global scale, especially in the remote areas with vulnerable and fragile ecosystems. The present review links together the source, fate, and environmental behavior of OPEs in remote areas, integrates the occurrence and profile data, summarizes their bioaccumulation, trophic transfer, and ecological risks, and finally points out the predominant pollution burden of OPEs among organic pollutants in remote areas. Given the relatively high contamination level and bioaccumulation/biomagnification behavior of OPEs, in combination with the sensitivity of endemic species in remote areas, more attention should be paid to the potential ecological risks of OPEs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.0c08822DOI Listing
August 2021

Spinal Nrf2 translocation may inhibit neuronal NF-κB activation and alleviate allodynia in a rat model of bone cancer pain.

J Neurochem 2021 Sep 31;158(5):1110-1130. Epub 2021 Jul 31.

Department of Anesthesiology and Pain Research Center, The First Hospital of Jiaxing or The Affiliated Hospital of Jiaxing University, Jiaxing, China.

Bone cancer pain (BCP) is a clinical pathology that urgently needs to be solved, but research on the mechanism of BCP has so far achieved limited success. Nuclear factor erythroid 2 (NFE2)-related factor 2 (Nrf2) has been shown to be involved in pain, but its involvement in BCP and the specific mechanism have yet to be examined. This study aimed to test the hypothesis that BCP induces the transfer of Nrf2 from the cytoplasm to the nucleus and further promotes nuclear transcription to activate heme oxygenase-1 (HO-1) and inhibit the activation of nuclear factor-kappa B (NF-κB) signalling, ultimately regulating the neuroinflammatory response. Von-Frey was used for behavioural analysis in rats with BCP, whereas western blotting, real-time quantitative PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect molecular expression changes, and immunofluorescence was used to detect cellular localization. We demonstrated that BCP induced increased Nrf2 nuclear protein expression with decreased cytoplasmic protein expression in the spinal cord. Further increases in Nrf2 nuclear protein expression can alleviate hyperalgesia and activate HO-1 to inhibit the expression of NF-κB nuclear protein and inflammatory factors. Strikingly, intrathecal administration of the corresponding siRNA reversed the above effects. In addition, the results of double immune labelling revealed that Nrf2 and NF-κB were coexpressed in spinal cord neurons of rats with BCP. In summary, these findings suggest that the entry of Nrf2 into the nucleus promotes the expression of HO-1, inhibiting activation of the NF-κB signalling pathway, reducing neuroinflammation and ultimately exerting an anti-nociceptive effect.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jnc.15468DOI Listing
September 2021

Influences of petroleum hydrocarbon pyrene on the formation, stability and antibacterial activity of natural Au nanoparticles.

Sci Total Environ 2021 Nov 3;795:148813. Epub 2021 Jul 3.

School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address:

The effect of pyrene on the formation of naturally Au nanoparticles (AuNPs) in the presence of humic acid (HA) under UV irradiation is described. TEM, EDS, FTIR and XPS were carried out to prove the formation of AuNPs and display their morphologies and formation mechanism. There are little differences between size, morphology and function groups of surface coated materials of AuNPs formed with and without pyrene. With the presence of HA, pyrene showed an inhibiting effect on the reduction of Au ion via competition for O, thereby decreasing the production of AuNPs. However, AuNPs formed by HA-pyrene showed higher stability than AuNPs formed by HA with the sedimentation rates of 4.13% and 13.68% respectively after 30-d standing. As for the antibacterial activities against Staphylococcus aureus and Escherichia coli, AuNPs formed by HA-pyrene were more toxic than AuNPs formed by HA. Meanwhile, changes of environmental factors such as temperature, pH and ionic strength exhibited similar influence trend on the formation of AuNPs in the presence and absence of pyrene. The results suggest that the typical petroleum hydrocarbon pyrene contained in spilled oil could influence the formation, fate and ecotoxicity of AuNPs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.148813DOI Listing
November 2021

[Perfluoroalkyl and polyfluoroalkyl substances in eggs: analytical methods and their application as pollutant bioindicator].

Se Pu 2021 Feb;39(2):184-196

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

Perfluoroalkyl and polyfluoroalkylated substances (PFASs) are environmentally persistent and biomagnified along food chains. They have been widely detected globally, even in the human body, and their potential toxicity has attracted great attention. Eggs are the origin of new life of ovipara and are rich in nutrients, thus they serve as one of the main protein sources for humans. Therefore, the level of pollutants in eggs can affect the reproduction of ovipara, and it is also related to human health by food intake. In recent years, poultry egg samples have been widely used in the assessment of biological and ecological pollution as a non-invasive biota matrix. At the same time, recent studies have used eggs to evaluate the developmental toxicity and associated health risks based on the pollutant levels in egg samples. In this study, the methods of sample pretreatment and instrumental detection of PFASs for egg samples are summarized. In addition, the application of eggs as a pollutants bioindicator of PFASs contamination has been discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3724/SP.J.1123.2020.09023DOI Listing
February 2021

Biotransformation of Timosaponin BII into Seven Characteristic Metabolites by the Gut Microbiota.

Molecules 2021 Jun 24;26(13). Epub 2021 Jun 24.

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, China.

Timosaponin BII is one of the most abundant Anemarrhena saponins and is in a phase II clinical trial for the treatment of dementia. However, the pharmacological activity of timosaponin BII does not match its low bioavailability. In this study, we aimed to determine the effects of gut microbiota on timosaponin BII metabolism. We found that intestinal flora had a strong metabolic effect on timosaponin BII by HPLC-MS/MS. At the same time, seven potential metabolites (M1-M7) produced by rat intestinal flora were identified using HPLC/MS-Q-TOF. Among them, three structures identified are reported in gut microbiota for the first time. A comparison of rat liver homogenate and a rat liver microsome incubation system revealed that the metabolic behavior of timosaponin BII was unique to the gut microbiota system. Finally, a quantitative method for the three representative metabolites was established by HPLC-MS/MS, and the temporal relationship among the metabolites was initially clarified. In summary, it is suggested that the metabolic characteristics of gut microbiota may be an important indicator of the pharmacological activity of timosaponin BII, which can be applied to guide its application and clinical use in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules26133861DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270264PMC
June 2021

Large-scale phenotypic drug screen identifies neuroprotectants in zebrafish and mouse models of retinitis pigmentosa.

Elife 2021 06 29;10. Epub 2021 Jun 29.

Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University, Baltimore, United States.

Retinitis pigmentosa (RP) and associated inherited retinal diseases (IRDs) are caused by rod photoreceptor degeneration, necessitating therapeutics promoting rod photoreceptor survival. To address this, we tested compounds for neuroprotective effects in multiple zebrafish and mouse RP models, reasoning drugs effective across species and/or independent of disease mutation may translate better clinically. We first performed a large-scale phenotypic drug screen for compounds promoting rod cell survival in a larval zebrafish model of inducible RP. We tested 2934 compounds, mostly human-approved drugs, across six concentrations, resulting in 113 compounds being identified as hits. Secondary tests of 42 high-priority hits confirmed eleven lead candidates. Leads were then evaluated in a series of mouse RP models in an effort to identify compounds effective across species and RP models, that is, potential pan-disease therapeutics. Nine of 11 leads exhibited neuroprotective effects in mouse primary photoreceptor cultures, and three promoted photoreceptor survival in mouse retinal explants. Both shared and complementary mechanisms of action were implicated across leads. Shared target tests implicated -dependent cell death in our zebrafish RP model. Complementation tests revealed enhanced and additive/synergistic neuroprotective effects of paired drug combinations in mouse photoreceptor cultures and zebrafish, respectively. These results highlight the value of cross-species/multi-model phenotypic drug discovery and suggest combinatorial drug therapies may provide enhanced therapeutic benefits for RP patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7554/eLife.57245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425951PMC
June 2021

Cloning and functional characterization of three flavonoid O-glucosyltransferase genes from the liverworts Marchantia emarginata and Marchantia paleacea.

Plant Physiol Biochem 2021 Sep 16;166:495-504. Epub 2021 Jun 16.

Key Laboratory of Chemical Biology of Natural Products, Ministry of Education, School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, China. Electronic address:

Flavonoid glycosides are important plant secondary metabolites with broad pharmacological activities. Flavonoid glycosides are generated from aglycones, in reactions catalyzed by typical uridine diphosphate-dependent glycosyltransferases (UGTs). Liverworts produce various types of flavonoid glycosides; however, only two UGTs have been characterized from liverworts to date. Here, we isolated three genes encoding UGTs (MeUGT1, MeUGT2, and MpalUGT1) from the liverwort species Marchantia emarginata and Marchantia paleacea through transcriptome sequencing. Recombinant MeUGT1, MeUGT2, and MpalUGT1 proteins heterologously produced in Escherichia coli exhibited catalytic activity towards multiple flavonoids. MeUGT1 and MpalUGT1 catalyzed the glycosylation of flavonols into the corresponding 3-O-glucosides with UDP-glucose as the sugar donor, while MeUGT2 exhibited a wider substrate specificity that included flavonols, flavones, and flavanones. When MeUGT2 was expressed in E. coli, the yield of flavonol 3-O-glucosides reached to 40-60% with feeding of the substrates kaempferol or quercetin under optimal conditions. Furthermore, heterologous expression of MeUGT1 in Arabidopsis thaliana increased the flavonol glycoside contents in the plants. Therefore, the UGTs characterized in this study could provide new data that will be useful for examining flavonoid biosynthesis in liverworts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.plaphy.2021.06.009DOI Listing
September 2021

Circular RNAs regulate cancer-related signaling pathways and serve as potential diagnostic biomarkers for human cancers.

Cancer Cell Int 2021 Jun 23;21(1):317. Epub 2021 Jun 23.

Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA.

Circular RNAs (circRNAs) are RNAs that have an important role in various pathological processes, including cancer. After the usage of high-throughput RNA sequencing, many circRNAs were found to be differentially expressed in various cancer cell lines and regulate cell signaling pathways by modulating particular gene expressions. Understanding their role in these pathways and what cancers they are found in can set the stage for identifying diagnostic and prognostic biomarkers and therapeutic targets of cancer. This paper will discuss which circRNAs are found in different cancers and what mechanisms they use to upregulate or downregulate certain cellular components.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12935-021-02017-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220689PMC
June 2021

CBP22, a Novel Bacteriocin Isolated from ZJU-F1, Protects against LPS-Induced Intestinal Injury through Maintaining the Tight Junction Complex.

Mediators Inflamm 2021 1;2021:8032125. Epub 2021 Jun 1.

Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou, China.

A novel bacteriocin secreted by ZJU-F1 was isolated using ammonium sulfate fractionation, cation exchange chromatography, affinity chromatography, and reverse-phase high-performance liquid chromatography (RP-HPLC). The bacteriocin, named CBP22, contained 22 amino acids with the sequence PSAWQITKCAGSIAWALGSGIF. Analysis of its structure and physicochemical properties indicated that CBP22 had a molecular weight of 2264.63 Da and a +1 net charge. CBP22 showed activity against , , and . The effects and potential mechanisms of bacteriocin CBP22 on the innate immune response were investigated with a lipopolysaccharide- (LPS-) induced mouse model. The results showed that pretreatment with CBP22 prevented LPS-induced impairment in epithelial tissues and significantly reduced serum levels of IgG, IgA, IgM, TNF-, and sIgA. Moreover, CBP22 treatment increased the expression of the zonula occludens and reduced permeability as well as apoptosis in the jejunum in LPS-treated mice. In summary, CBP22 inhibits the intestinal injury and prevents the gut barrier dysfunction induced by LPS, suggesting the potential use of CBP22 for treating intestinal damage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/8032125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187061PMC
June 2021

Hierarchical CoFe LDH/MOF nanorods array with strong coupling effect grown on carbon cloth enables efficient oxidation of water and urea.

Nanotechnology 2021 Jul 2;32(38). Epub 2021 Jul 2.

School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, People's Republic of China.

Oxygen evolution reaction (OER) and urea oxidation reaction (UOR) play important roles in the fields of hydrogen energy production and pollution treatment. Herein, a facile one-step chemical etching strategy is provided for fabricating one-dimensional hierarchical nanorods array composed of CoFe layered double hydroxide (LDH)/metal-organic frameworks (MOFs) supported on carbon cloth as efficient and stable OER and UOR catalysts. By precisely controlling the etching rate, the ligands from Co-MOFs are partially removed, the corresponding metal centers then coordinate with hydroxyl ions to generate ultrathin amorphous CoFe LDH nanosheets. The resultant CoFe LDH/MOFs catalyst possesses large active surface area, enhanced conductivity and extended electron/mass transfer channels, which are beneficial for catalytic reactions. Additionally, the intimate contact between CoFe LDH and MOFs modulates the local electronic structure of the catalytic active site, leading to enhanced adsorption of oxygen-containing intermediates to facilitate fast electrocatalytic reaction. As a result, the optimized CoFe LDH/MOF-0.06 exhibits superior OER activity with a low overpotential of 276 at a current density of 10 mA cmwith long-term durability. Additionally, it merely requires a voltage of 1.45 V to obtain 10 mA cmin 1 M KOH solution with 0.33 urea and is 56 mV lower than the one in pure KOH. The work presented here may hew out a brand-new route to construct multi-functional electrocatalysts for water splitting, COreduction, nitrogen reduction reactions and so on.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/ac0b65DOI Listing
July 2021

A Cu O-derived Polymeric Carbon Nitride Heterostructured Catalyst for the Electrochemical Reduction of Carbon Dioxide to Ethylene.

ChemSusChem 2021 Aug 28;14(15):3190-3197. Epub 2021 Jun 28.

Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, P.R. China.

The electroreduction of carbon dioxide to hydrocarbons has been proposed as a promising way to utilize CO and maintain the ecosystem carbon balance. However, the selective reduction of CO to C hydrocarbons is still challenging. In this study, a highly efficient heterostructured catalyst has been developed, composed of a carbon nitride (CN)-encapsulated copper oxide hybrid (Cu O/CN). The interaction between the metal and carbon nitride in the heterostructured catalysts improves the intrinsic electrical conductivity and the charge transfer processes at metal-support interfaces. A remarkable enhancement in the selectivity of hydrocarbons is achieved with these modified Cu-based electrocatalysts, with an onset potential of -0.4 V and high C H faradaic efficiency of 42.2 %, and these catalysts can also effectively suppress H evolution during the CO reduction reaction. This work provides a simple and cost-effective method for synthesizing CN-encapsulated catalysts that provides the possibility of efficiently converting CO into C hydrocarbons.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cssc.202100659DOI Listing
August 2021
-->