Publications by authors named "Jie Feng"

717 Publications

Characteristics of the Soil Microbial Communities in Different Slope Positions along an Inverted Stone Slope in a Degraded Karst Tiankeng.

Biology (Basel) 2021 May 27;10(6). Epub 2021 May 27.

College of Environment and Resources, Fuzhou University, Fuzhou 350116, China.

The underground forests developed on inverted stone slopes in degraded karst tiankengs are important areas for biodiversity conservation, but the microbial community profiles have not been sufficiently characterized. Thus, we investigated the soil microbial communities at four sites (at the bottom of the slope (BS), in the middle of the slope (MS), in the upper part of the slope (US) and outside the tiankeng (OT)) in the Shenxiantang tiankeng. The dominant phyla in the inverted stone slope were Proteobacteria, Actinobacteria, and Acidobacteria, and the relative abundance were different in different slope positions. The Shannon-Wiener diversity index of the microbial community was significantly greater for the US site than for the MS or BS sites. The metabolism functional pathways (including C/N cycle) were more abundant at the BS site. Total nitrogen and pH were the dominant factors in determining the distribution of the microbial community along an inverted stone slope. These results suggest that topographic heterogeneity can influence the variations in the soil microbial structure, diversity, and function in degraded karst tiankengs and emphasized the ecological value of inverted stone slopes within karst tiankengs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/biology10060474DOI Listing
May 2021

Case Report: Aicardi-Goutières Syndrome and Singleton-Merten Syndrome Caused by a Gain-of-Function Mutation in IFIH1.

Front Genet 2021 13;12:660953. Epub 2021 May 13.

Department of Neurology, Xiangya Hospital, Central South University, Changsha, China.

The gene encodes melanoma differentiation-associated gene 5 (MDA5) and has been associated with Aicardi-Goutières syndrome (AGS), Singleton-Merten syndrome (SMS), and other autoimmune diseases. The mechanisms responsible for how a functional change in a single gene can cause so many different phenotypes remain unknown. Moreover, there is significant controversy as to whether these distinct phenotypes represent the same disease continuum or mutation-specific disorders. Here, we describe the case of a patient with a novel c.1465G > T (p.Ala489Ser) mutation in the gene. The patient presented with spastic paraplegia, dystonia, psychomotor retardation, joint deformities, intracranial calcification, abnormal dentition, characteristic facial features, lymphadenopathy, and autoimmunity. His phenotype appeared to represent an overlap of the phenotypes for AGS and SMS. The patient also experienced unexplained pancytopenia, suggesting that the hemic system may have been affected by a gain-of-function mutation in the gene. In summary, we provide further evidence that SMS and AGS exhibit the same disease spectrum following a gain-of-function mutation in the gene. Our data highlight the genetic heterogeneity of these conditions and expand our knowledge of differential phenotypes created by gain-of-function mutation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2021.660953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155672PMC
May 2021

Spatiotemporal quantification of inoculum in relation to clubroot development under inoculated and naturally infested field conditions.

Plant Dis 2021 May 21. Epub 2021 May 21.

Shenyang Agricultural University, 98428, Plant Pathology, No. 120 Dongling Road, Shenhe, Shenyang, Liaoning, China, 110866;

Clubroot caused by is a destructive disease of cruciferous plants worldwide. A quantitative PCR (qPCR) system specific to was developed. Analysis of the qPCR sensitivity indicated that the lower limit of detection was 1 × 10 resting spores/mL, 1 × 10 spores/g soil, and 1 × 10 spores/g roots and seeds. The regression curves generated from the qPCR data of different samples had a parallel relationship. The difference between the theoretical and actual concentrations was lowest at 1 × 10 spores/g sample, compared to other concentrations. The biomass in soil and plant root tissues after inoculated with different spore concentrations was correlated. A correlation analysis confirmed that the clubroot incidence and disease index at six weeks after inoculation increased as the spore concentration increased. Under field conditions, the natural inoculum density of population decreased at early stage and then increased with mainly being detected at a soil depth of 0-50 cm. The horizontal distribution of varied in the field with occurrences of hot spots. This study established a qPCR-based method for quantitative detection of clubroot. The developed assay is useful for monitoring the spatiotemporal dynamics of in the field. It may also be applicable for clubroot forecasting as a part of proactive disease management.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1094/PDIS-03-21-0653-REDOI Listing
May 2021

Src family kinases involved in the differentiation of human preadipocytes.

Mol Cell Endocrinol 2021 May 14;533:111323. Epub 2021 May 14.

Nanjing Maternal and Child Health Medical Institute, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu 210004, China. Electronic address:

Background: Obesity is characterized by the excess accumulation of white adipose tissue (WAT). Src family kinases (SFKs) are non-receptor tyrosine kinases consisting of eight members (SRC, FYN, YES1, HCK, LCK, LYN, FGR and BLK) that have been studied extensively in mammalian cells. Although individual members in murine cells provide some clues that are associated with the regulation of adipogenesis, the specific role of this family in adipocyte differentiation has rarely been assessed, especially in human adipocytes.

Methods: Herein, we first explored the expression profiles of SFKs during human preadipocyte differentiation. Then, we used the pyrazolo-pyrimidinyl-amine compound PP1, a potent SFK inhibitor, to evaluate the function of SFKs during adipocyte differentiation. Furthermore, we adopted a loss-of-function strategy with siRNAs to determine the role of FGR in adipocyte differentiation.

Results: Here, we found that SRC, FYN, YES1, LYN and FGR were expressed in human preadipocytes and induced after the initiation of differentiation. Furthermore, the SFK inhibitor PP1 suppressed adipocyte differentiation. We also found that PP1 significantly suppressed the SFK activity in preadipocytes and decreased the expression of adipogenic genes in early and late differentiation. Given that FGR exhibited the most expression enhancement in mature adipocytes, we focused on FGR and found that its knockdown reduced lipid accumulation and adipogenic gene expression.

Conclusions: Collectively, these findings suggest that SFKs, especially FGR, are involved in the differentiation of human preadipocytes. Our results lay a foundation for further understanding the role of SFKs in adipocyte differentiation and provide new clues for anti-obesity therapies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.mce.2021.111323DOI Listing
May 2021

Prevalence and Risk Factors of Type 2 Diabetes and Prediabetes Among 53,288 Middle-Aged and Elderly Adults in China: A Cross-Sectional Study.

Diabetes Metab Syndr Obes 2021 3;14:1975-1985. Epub 2021 May 3.

Department of Nephrology, The Second Clinical Medical Institution of North Sichuan Medical College (Nanchong Central Hospital) and Nanchong Key Laboratory of Basic Science & Clinical Research on Chronic Kidney Disease, Nanchong, Sichuan, People's Republic of China.

Background: Diabetes is a metabolic disorder that causes a heavy burden on healthcare systems worldwide. The aim of this study was to determine the prevalence of type 2 diabetes and prediabetes and its associated factors among eight communities in Nanchong, China.

Methods: This was an observational cross-sectional study conducted throughout eight communities in Nanchong, China. The participants were 53,288 individuals aged 45 years or older. The participants' characteristics, comorbidities, health behaviors, family history, and dietary intake were assessed. Multinomial logistic regression models were fitted to identify factors associated with type 2 diabetes and prediabetes.

Results: The prevalence of diabetes and prediabetes was 13.9% (95% confidence interval [CI], 13.6-14.2) and 3.1% (95% CI, 2.9-3.2) of the population, respectively. After adjusting for other risk factors, advanced age, overweight, obesity, abdominal obesity, comorbidities, smoking, a family history of diabetes, and Chinese cooking vegetable intake were associated with an increased risk of type 2 diabetes and prediabetes.

Conclusion: The prevalence of type 2 diabetes in the Chinese population is rising compared with data from the past. The risk factors of type 2 diabetes and prediabetes identified in this study will aid the identification of individuals at a high-risk of diabetes and the implementation of effective health promotion programs and campaigns.

Clinical Trial Registry Number: ChiCTR-HOC-17013200.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/DMSO.S305919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104985PMC
May 2021

Atroposelective Synthesis of Axially Chiral 4-Aryl α-Carbolines via -Heterocyclic Carbene Catalysis.

Org Lett 2021 Jun 11;23(11):4267-4272. Epub 2021 May 11.

State Key Laboratory of Natural Medicines, Department of Organic Chemistry, China Pharmaceutical University, Nanjing 210009, P. R. China.

The first catalytic asymmetric construction of axially chiral 4-aryl α-carboline skeletons has been accomplished through an -heterocyclic carbene (NHC)-catalyzed atroposelective formal [3 + 3] annulation of 4-nitrophenyl 3-arylpropiolates with 2-sulfonamidoindolines. The synthetic utility of the title compounds has been demonstrated by the diverse late-stage structural modifications. Density functional theory calculations were also conducted to illuminate the key factors for controlling the origin of the enantioselectivity. This strategy not only provides an efficient pathway to access axially chiral α-carboline atropisomers but also offers a novel catalytic enantioselective mode for the construction of axially chiral heterobiaryls by using NHC-bound alkynyl acylazoliums.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.orglett.1c01221DOI Listing
June 2021

Long-term safety, efficacy, and tolerability of levetiracetam in pediatric patients with epilepsy in Uygur, China: A retrospective analysis.

Epilepsy Behav 2021 Jul 5;120:108010. Epub 2021 May 5.

Department of Pharmacy, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Province 830001, China; Institute of Clinical Pharmacy, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Province 830001, China. Electronic address:

Purpose: Levetiracetam is approved as an add-on therapy to treat refractory partial seizures in pediatric patients over four years old. The efficacy and safety in pediatric patients with epilepsy in Uygur, China, is unknown. Therefore, the objective of this study was to investigate the safety, efficacy, and tolerability of levetiracetam in pediatric patients with epilepsy in Uygur, China.

Methods: This retrospective study compared the efficacy and safety of levetiracetam monotherapy and in combination with other antiseizure medications (ASMs) in 1055 pediatric patients with epilepsy treated with levetiracetam. The seizure frequencies at 1, 2, and 3 years after starting levetiracetam therapy were recorded and compared with the baseline yearly frequency. Safety variables included the incidence and type of adverse reactions.

Results: A total of 680 (64%) pediatric patients responded to levetiracetam therapy with a more than 50% reduction in the frequency of seizures. Seizure-free rates increased over time, 13%, 15%, and 18% at 1, 2, and 3 years, respectively. The number of baseline ASMs and the order of levetiracetam introduction were highly significant, impacting the likelihood of seizure remission during a 3-year follow-up period (p < 0.001). During levetiracetam treatment, 233 pediatric patients (22%) experienced at least one adverse reaction.

Conclusion: These significant findings indicate that levetiracetam is likely to become a widely prescribed ASM for epilepsy in pediatric clinical practice because of its long-term safety, efficacy, and tolerability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.yebeh.2021.108010DOI Listing
July 2021

Network pharmacology-based identification of the key mechanism of quercetin acting on hemochromatosis.

Metallomics 2021 Jun;13(6)

College of Animal Sciences, Zhejiang University, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou 310058, China.

Hemochromatosis is an iron overload disease, which lacks nutritional intervention strategies. This study explored the protective effect of quercetin on hemochromatosis and its possible mechanism through network pharmacology. We used Online Mendelian Inheritance in Man to screen the disease targets of hemochromatosis, and further constructed a potential protein interaction network through STITCH. The above-mentioned targets revealed by Gene enrichment analysis have played a significant role in ferroptosis, mineral absorption, basal cell carcinoma, and related signal pathways. Besides, the drug likeness of quercetin obtained by Comparative Toxicogenomics Database was evaluated by Traditional Chinese Medicine Systems Pharmacology, and potential drug targets identified by PharmMapper and similar compounds identified by PubChem were selected for further research. Moreover, gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed the relationship between quercetin and glycosylation. Furthermore, we performed experiments to verify that the protective effect of quercetin on iron overload cells is to inhibit the production of reactive oxygen species, limit intracellular iron, and degrade glycosaminoglycans. Finally, iron-induced intracellular iron overload caused ferroptosis, and quercetin and fisetin were potential ferroptosis inhibitors. In conclusion, our study revealed the correlation between hemochromatosis and ferroptosis, provided the relationship between the target of quercetin and glycosylation, and verified that quercetin and its similar compounds interfere with iron overload related disease. Our research may provide novel insights for quercetin and its structurally similar compounds as a potential nutritional supplement for iron overload related diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/mtomcs/mfab025DOI Listing
June 2021

Compliance with the WHO 24-hour movement guidelines and associations with body weight status among preschool children in Hong Kong.

Appl Physiol Nutr Metab 2021 May 4. Epub 2021 May 4.

The Chinese University of Hong Kong, Department of Sports Science and Physical Education, Shatin, N.T., Hong Kong;

This study aimed to investigate the extent to which preschool children in Hong Kong meet the WHO 24-hour movement guidelines and to examine the associations between meeting the guidelines and body weight status. Data from 251 preschoolers (141 boys) aged between three and six years old were analysed. The children wore an activPAL for seven consecutive days to measure physical activity and sleep. Their screen time was reported by their parents using validated questions. Their body weight status was classified as underweight, normal weight or overweight/obese. A total of 2.9% of the preschoolers met all three movement guidelines. Children in compliance with the physical activity, screen time and sleep guidelines accounted for 14.5%, 67.4% and 39.0% of the sample, respectively. There was no significant association between meeting the individual or combined guidelines and body mass index or the odds ratio for being of normal weight. Meeting more guidelines was not associated with body mass index or the odds ratio for being normal weight. Compliance with the 24-hour movement guidelines was extremely low among preschool children in Hong Kong. Meeting the 24-hour movement guidelines was not associated with a favourable weight status among preschoolers. Novelty • Compliance with the WHO 24-hour movement guidelines was extremely low for the early years in Hong Kong. • Further evidence is needed to understand the associations between meeting the guidelines and weight status among preschoolers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1139/apnm-2020-1035DOI Listing
May 2021

Contamination and translocation of deoxynivalenol and its derivatives associated with Fusarium crown rot of wheat in Northern China.

Plant Dis 2021 May 4. Epub 2021 May 4.

Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, Beijing, China;

Fusarium crown rot (FCR) is one of the most important wheat diseases in Northern China. The main causal agent of FCR, , can produce mycotoxins such as type B trichothecenes. Therefore, FCR could be an additional source of mycotoxin contamination during wheat production. Field inoculation experiments demonstrated that FCR disease severity strongly impacts the distribution pattern of trichothecenes in different wheat tissues. Mycotoxins were mainly observed in lower internodes and a low amount was detected in the upper parts above the 4th internode. However, high levels of trichothecene accumulation were detected in the upper segments of wheat plants under field conditions, which would threaten the feed production. The variation of mycotoxin content among sampling sites indicated that besides disease severity, other factors like climate, irrigation, and fungicide application may influence the mycotoxin accumulation in wheat. A comprehensive survey of DON and its derivatives in wheat heads with FCR symptoms in natural fields was conducted in 80 sites of seven provinces in Northern China. Much higher levels of mycotoxin were observed than those in inoculation experiments. The mycotoxin content varied greatly among sampling sites, but no significant differences were observed if compared at province level, which indicated that the variation is mainly due to local conditions. Trace amounts of mycotoxin appeared to be translocated to grains, indicating that FCR infection in natural fields poses a relatively small threat to contamination of grains, but a larger amount to plant parts that may be used as animal feed. To our knowledge, this is the first report of trichothecene accumulation in wheat stems, heads, and grains following FCR infection in natural field condition. These investigations provide novel insights into food and feed safety risk caused by FCR in Northern China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1094/PDIS-03-21-0612-REDOI Listing
May 2021

Protein profiling identified mitochondrial dysfunction and synaptic abnormalities after dexamethasone intervention in rats with traumatic brain injury.

Neural Regen Res 2021 Dec;16(12):2438-2445

Department of Neurotrauma, Beijing Key Laboratory of Central Nervous System Injury, Beijing Neurosurgical Institute; Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University; Center for Nerve Injury and Repair, Beijing Institute of Brain Disorders; China National Clinical Research Center for Neurological Diseases, Beijing, China.

Dexamethasone has been widely used after various neurosurgical procedures due to its anti-inflammatory property and the abilities to restore vascular permeability, inhibit free radicals, and reduce cerebrospinal fluid production. According to the latest guidelines for the treatment of traumatic brain injury in the United States, high-dose glucocorticoids cause neurological damage. To investigate the reason why high-dose glucocorticoids after traumatic brain injury exhibit harmful effect, rat controlled cortical impact models of traumatic brain injury were established. At 1 hour and 2 days after surgery, rat models were intraperitoneally administered dexamethasone 10 mg/kg. The results revealed that 31 proteins were significantly upregulated and 12 proteins were significantly downregulated in rat models of traumatic brain injury after dexamethasone treatment. The Ingenuity Pathway Analysis results showed that differentially expressed proteins were enriched in the mitochondrial dysfunction pathway and synaptogenesis signaling pathway. Western blot analysis and immunohistochemistry results showed that Ndufv2, Maob and Gria3 expression and positive cell count in the dexamethasone-treated group were significantly greater than those in the model group. These findings suggest that dexamethasone may promote a compensatory increase in complex I subunits (Ndufs2 and Ndufv2), increase the expression of mitochondrial enzyme Maob, and upregulate synaptic-transmission-related protein Gria3. These changes may be caused by nerve injury after traumatic brain injury treatment by dexamethasone. The study was approved by Institutional Ethics Committee of Beijing Neurosurgical Institute (approval No. 201802001) on June 6, 2018.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4103/1673-5374.313047DOI Listing
December 2021

Response by Li et al to Letter Regarding Article, "gp130 Controls Cardiomyocyte Proliferation and Heart Regeneration".

Circulation 2021 Apr 12;143(15):e813-e814. Epub 2021 Apr 12.

State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1161/CIRCULATIONAHA.120.052610DOI Listing
April 2021

Effects of enzymatic reaction on the generation of key aroma volatiles in shiitake mushroom at different cultivation substrates.

Food Sci Nutr 2021 Apr 26;9(4):2247-2256. Epub 2021 Feb 26.

Institute of Edible Fungi Shanghai Academy of Agricultural Sciences National Engineering Research Center of Edible Fungi Key Laboratory of Edible Fungi Resources and Utilization (South) Ministry of Agriculture Shanghai China.

Aroma is an important factor affecting mushroom character and quality. According to the different reaction pathway, the key aroma metabolites (sulfur and eight-carbon volatiles) formation can be classified into enzymatic reactions and nonenzymatic reactions. Aroma volatiles are generated from precursors via the biocatalytic activities of various synthases during the growth stages of shiitake mushrooms. Understanding the specific relationships between the key aroma metabolites and their synthases is key to improving shiitake mushroom quality. At the same time, to reduce forest logging and burning of agricultural by-products in farmland, agricultural by-products have been applied to shiitake mushroom cultivation. Nevertheless, how to further improve the production of aroma volatiles in mushroom cultivated with agricultural waste is still a challenge. In order to understand the biosynthesis of volatiles via enzymatic reactions and screen the agricultural by-products that can improve the production of aroma volatiles in mushroom cultivation, the mechanism of producing aroma volatiles needs to be further elucidated. In this study, the activities and gene expression levels of the key synthases involved in volatile metabolism, the contents of key aroma volatiles, and the correlations between related synthetase, volatiles, and cultivation substrate (CS) were investigated. Network models for visualizing the links between synthetase, volatiles, and CSs were built through partial least squares (PLS) regression analysis. The correlation coefficients among three related synthetase and enzymatic gene expression were high, and the combined effects of multiple synthetase promoted the production of volatiles. PLS analysis showed that the corncob and corn meal were more related to the production of volatiles and synthetase gene expression, and they can be added to the CSs as flavor promoting substances. The enrichment of key aroma volatiles in shiitake mushroom cultivated by the gradient of 20% corn meal combination CS was noticeable.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/fsn3.2198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020957PMC
April 2021

Selection of copper and zinc dosages in pig diets based on the mutual benefit of animal growth and environmental protection.

Ecotoxicol Environ Saf 2021 Apr 8;216:112177. Epub 2021 Apr 8.

College of Animal Sciences, Zhejiang University, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou, China. Electronic address:

Dietary copper and zinc additives facilitate the growth and development of animals, but heavy metal in feces threatens the ecological environment, and balance is the key to solving the problem. In this study, a trial of 2000 pigs (early nursery, 9-15 kg; late nursery, 15-25 kg; grower: 25-60 kg) was conducted to analyze the effects of different diets (gradient dosage of copper and zinc additives) on growth performance, antioxidant performance, immune function, and fecal heavy metal excretion of piglets and growing pigs. Although no significant differences were observed in average daily gain (ADG) and average daily feed intake (ADFI) between treatments during the entire nursery-grower period, the addition of appropriate high doses of copper and zinc to the diet had a beneficial effect on the antioxidant status and immune function of weaned piglets. Especially at early nursery, compared with the low-copper group (5 mg/kg Cu), the high-copper group (120 mg/kg Cu) could significantly increase the peroxidase (POD), glutathione peroxidase (GSH-PX), total antioxidant capacity (T-AOC), catalase (CAT) and copper/zinc superoxide dismutase (Cu/Zn-SOD), cortisol in the serum. Moreover, the addition of zinc and copper in the diet not only increased the concentration of corresponding trace elements in the serum, but also affected the concentration of other trace elements in the serum. The reduction of copper and zinc content in the diet contributed to reducing the copper and zinc content in feces. In conclusion, we have formulated the mutual benefit dosages of copper and zinc (9-15 kg: 5 mg/kg Cu and 50 mg/kg Zn; 15-25 kg: 4 mg/kg Cu and 50 mg/kg Zn; 25-60 kg: 4 mg/kg Cu and 10 mg/kg Zn) for weaning piglets and growing pigs, which would help ensure the healthy growth of animals and reduce environmental heavy metal residues. CAPSULE: This study developed a mutually beneficial dose of copper and zinc in pig diets, which promotes animal growth and protects the environment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2021.112177DOI Listing
April 2021

Core-Shell-Structured Covalent-Organic Framework as a Nanoagent for Single-Laser-Induced Phototherapy.

ACS Appl Mater Interfaces 2021 Apr 7;13(15):17243-17254. Epub 2021 Apr 7.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, P. R. China.

Imaging-guided phototherapy, including photothermal therapy and photodynamic therapy, has been emerging as a promising avenue for precision cancer treatment. However, the utilization of a single laser to induce combination phototherapy and multiple-model imaging remains a great challenge. Herein, we report, the first of its kind, a covalent-organic framework (COF)-based magnetic core-shell nanocomposite, , that is used as a multifunctional nanoagent for cancer theranostics under single 660 nm NIR irradiation. Besides significant photothermal and photodynamic effects, it still permits triple-modal magnetic resonance/photoacoustic/near-infrared thermal (IR) imaging due to its unequaled magnetic and optical performance. We believe that the results obtained herein could obviously promote the application of COF-based multifunctional nanomaterials in cancer theranostics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c01125DOI Listing
April 2021

Development and Optimization of the Triterpenoid and Sterol Production Process with Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum Strain G0017 (Agaricomycetes), in Liquid Submerged Fermentation at Large Scale.

Int J Med Mushrooms 2021 ;23(3):43-53

Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture; National Engineering Research Center of Edible Fungi, Key Laboratory of Agricultural Genetics and Breeding of Shanghai; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Ganoderma lucidum mycelia are rich in active substances such as triterpenoids and sterols. However, reports on the development of effective submerged fermentation processes are lacking and the resulting total triterpene and sterol yield is still quite low. In this study, a new G. lucidum strain G0017 mycelium isolated by screening was studied in a 3-L fermenter to investigate the effect of aeration rate in liquid submerged fermentation production of triterpenoids and sterols. By fitting the specific mycelial growth rate and the specific production rate of the triterpenoid and sterol model, an effective multistage aeration rate control process for triterpenoid and sterol fermentation production was developed. This process was validated and proven in 3-L and 50-L fermenters. The resulting yields of triterpenoids and sterols were 3.34 and 3.46 g/L, respectively, which were 69.54% and 75.63% higher than the fixed aeration rate of 1.50 volume of air per volume of liquid per minute. This optimized fermentation production process conceivably could be applied to larger-scale industrial production and perhaps also to improve liquid submerged fermentation processes with relevant edible and medicinal mushrooms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1615/IntJMedMushrooms.2021037830DOI Listing
January 2021

Transplantation of Neonatal Mouse Cardiac Macrophages into Adult Mice.

J Vis Exp 2021 03 20(169). Epub 2021 Mar 20.

Fuwai Hospital & Chinese Academy of Medical Sciences, State Key Laboratory of Cardiovascular Disease, Cardiovascular Institute, National Center for Cardiovascular Diseases, Peking Union Medical College; National Health Commission Key Laboratory of Cardiovascular Regenerative Medicine, Fuwai Central-China Hospital, Central-China Subcenter of National Center for Cardiovascular Diseases;

In an injured neonatal myocardium, macrophages facilitate cardiomyocyte proliferation and angiogenesis and promote heart regeneration. The present study reveals that transplantation of neonatal cardiac macrophages recruited by injury promotes adult heart regeneration after myocardial infarction with improvement of cardiac function and cardiomyocyte proliferation. The results indicate that neonatal cardiac macrophage transplantation could be a promising strategy for cardiac injury treatment. Here, we provide the technical details, including the isolation of neonatal cardiac macrophages from apical resection-injured neonatal mouse hearts, the transplantation of macrophages into myocardial-infarcted adult mice, and the estimation of heart regeneration after a macrophage graft.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3791/62108DOI Listing
March 2021

Monitoring atmospheric Kr by atom counting.

J Environ Radioact 2021 Jul 1;233:106604. Epub 2021 Apr 1.

Hefei National Laboratory for Physical Sciences at the Microscale, CAS Center for Excellence in Quantum Information and Quantum Physics, University of Science and Technology of China, 96 Jinzhai Road, Hefei, 230026, China.

Radioactive Kr is a major gaseous fission product emitted into the air by the nuclear fuel reprocessing industry. Measuring atmospheric Kr has applications in environmental monitoring, atmospheric transport model validation and dating of environmental water samples, including groundwater, sea water and glacier ice. We present an ultra-sensitive method for fast analysis of atmospheric Kr at 10 parts per trillion level. This method is based on laser cooling and trapping and is capable of counting individual Kr atoms. Measurements at the 3% precision level can be made on krypton extracted from 1L STP of air with a turnaround time of 1.5 h. Moreover, we have realized a system for continuous air sampling over days to weeks. Based on this atom-counting technology and a portable air sample integrator we have realized atmospheric Kr baseline monitoring in Hefei, China, for over 20 months. The technological advances presented in this work lay the ground for a global atmospheric Kr monitoring network.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jenvrad.2021.106604DOI Listing
July 2021

A Magnetically Actuated Superhydrophobic Ratchet Surface for Droplet Manipulation.

Micromachines (Basel) 2021 Mar 19;12(3). Epub 2021 Mar 19.

Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

A water droplet dispensed on a superhydrophobic ratchet surface is formed into an asymmetric shape, which creates a Laplace pressure gradient due to the contact angle difference between two sides. This work presents a magnetically actuated superhydrophobic ratchet surface composed of nanostructured black silicon strips on elastomer ridges. Uniformly magnetized NdFeB layers sputtered under the black silicon strips enable an external magnetic field to tilt the black silicon strips and form a superhydrophobic ratchet surface. Due to the dynamically controllable Laplace pressure gradient, a water droplet on the reported ratchet surface experiences different forces on two sides, which are explored in this work. Here, the detailed fabrication procedure and the related magnetomechanical model are provided. In addition, the resultant asymmetric spreading of a water droplet is studied. Finally, droplet impact characteristics are investigated in three different behaviors of deposition, rebound, and penetration depending on the impact speed. The findings in this work are exploitable for further droplet manipulation studies based on a dynamically controllable superhydrophobic ratchet surface.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/mi12030325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003513PMC
March 2021

Dietary copper/zinc-loaded montmorillonite improved growth performance and intestinal barrier and changed gut microbiota in weaned piglets.

J Anim Physiol Anim Nutr (Berl) 2021 Apr 1. Epub 2021 Apr 1.

College of animal science, Zhejiang University, Hangzhou, China.

The effects of copper/zinc-loaded montmorillonite (Cu/Zn-Mt) on growth performance, intestinal barrier and gut microbiota of weaned pigs were investigated in the present study. A total of 108 piglets (Duroc × Landrace × Yorkshire; 6.36 kg; weaned at 21 ± 1 d age) were used in this experiment. The pigs were randomly assigned to three treatments with six replicates, six pigs in each replicate. The three treatments were as follows: (1) control group: basal diet; (2) Cu/Zn-Mt group: basal diet supplemented with 39 mg/kg Cu and 75 mg/kg Zn as Cu/Zn-Mt; and (3) Cu +Zn +Mt group: basal diet supplemented with the mixture of copper sulphate, zinc sulphate and montmorillonite (equivalent to the copper and zinc in the Cu/Zn-Mt treatment). The results indicated that, compared with the pigs from control group, average daily gain and gain: feed ratio were increased and the faecal score on days 7 and 14 after weaning was decreased by supplementation of Cu/Zn-Mt; intestinal transepithelial electrical resistance (TER) and expressions of tight junction protein claudin-1 and zonula occludens-1 were increased, and intestinal permeability of fluorescein isothiocyanate-dextran 4 kDa was decreased by supplementation with Cu/Zn-Mt. According to the Illumina-based sequencing results, Cu/Zn-Mt supplementation increased the relative abundance of core bacteria (Lactococcus, Bacillus) at genus level and decreased the potentially pathogenic bacteria (Streptococcus and Pseudomonas) in colon of weaned piglets. However, the piglets fed with the mixture of copper sulphate, zinc sulphate and montmorillonite showed no effects in above parameters in comparison with the pigs from control group. In conclusion, dietary Cu/Zn-Mt could improve growth performance, decrease the diarrhoea and improve intestinal barrier and bacterial communities of weaned pigs. The results indicated that 'loading' of montmorillonite with Zn and Cu changed not only its chemical but also its nutritional properties.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jpn.13522DOI Listing
April 2021

Correct identification of incomplete Kawasaki disease.

J Int Med Res 2021 Mar;49(3):3000605211001712

Department of Ultrasound, Weifang People's Hospital, Weifang City, Shandong Province, China.

Incomplete Kawasaki disease (IKD) is characterized by a longer fever time, younger age of onset, and higher incidence of coronary artery disease compared with complete Kawasaki disease. Kawasaki disease is often difficult to diagnose early because of its incomplete clinical symptoms. This issue could delay treatment and harm the health of the child. This article reviews the clinical characteristics and pathogenesis of IKD to help clinicians understand the symptoms of IKD, make the correct diagnosis, and provide timely treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/03000605211001712DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020237PMC
March 2021

Genome assembly and transcriptome analysis provide insights into the antischistosome mechanism of Microtus fortis.

J Genet Genomics 2020 Dec 9;47(12):743-755. Epub 2021 Feb 9.

Bio-Med Big Data Center, CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China; Shanghai Center for Bioinformation Technology, Shanghai Academy of Science and Technology, Shanghai 201203, China; Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, Zhejiang 330106, China. Electronic address:

Microtus fortis is the only mammalian host that exhibits intrinsic resistance against Schistosoma japonicum infection. However, the underlying molecular mechanisms of this resistance are not yet known. Here, we perform the first de novo genome assembly of M. fortis, comprehensive gene annotation analysis, and evolution analysis. Furthermore, we compare the recovery rate of schistosomes, pathological changes, and liver transcriptomes between M. fortis and mice at different time points after infection. We observe that the time and type of immune response in M. fortis are different from those in mice. M. fortis activates immune and inflammatory responses on the 10th day post infection, such as leukocyte extravasation, antibody activation, Fc-gamma receptor-mediated phagocytosis, and the interferon signaling cascade, which play important roles in preventing the development of schistosomes. In contrast, an intense immune response occurrs in mice at the late stages of infection and could not eliminate schistosomes. Infected mice suffer severe pathological injury and continuous decreases in cell cycle, lipid metabolism, and other functions. Our findings offer new insights into the intrinsic resistance mechanism of M. fortis against schistosome infection. The genome sequence also provides the basis for future studies of other important traits in M. fortis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jgg.2020.11.009DOI Listing
December 2020

Multi-Omics Data Fusion via a Joint Kernel Learning Model for Cancer Subtype Discovery and Essential Gene Identification.

Front Genet 2021 4;12:647141. Epub 2021 Mar 4.

Changsha Municipal Center of Disease Control, Changsha, China.

The multiple sources of cancer determine its multiple causes, and the same cancer can be composed of many different subtypes. Identification of cancer subtypes is a key part of personalized cancer treatment and provides an important reference for clinical diagnosis and treatment. Some studies have shown that there are significant differences in the genetic and epigenetic profiles among different cancer subtypes during carcinogenesis and development. In this study, we first collect seven cancer datasets from the Broad Institute GDAC Firehose, including gene expression profile, isoform expression profile, DNA methylation expression data, and survival information correspondingly. Furthermore, we employ kernel principal component analysis (PCA) to extract features for each expression profile, convert them into three similarity kernel matrices by Gaussian kernel function, and then fuse these matrices as a global kernel matrix. Finally, we apply it to spectral clustering algorithm to get the clustering results of different cancer subtypes. In the experimental results, besides using the -value from the Cox regression model and survival analysis as the primary evaluation measures, we also introduce statistical indicators such as Rand index (RI) and adjusted RI (ARI) to verify the performance of clustering. Then combining with gene expression profile, we obtain the differential expression of genes among different subtypes by gene set enrichment analysis. For lung cancer, GMPS, EPHA10, C10orf54, and MAGEA6 are highly expressed in different subtypes; for liver cancer, CMYA5, DEPDC6, FAU, VPS24, RCBTB2, LOC100133469, and SLC35B4 are significantly expressed in different subtypes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2021.647141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969795PMC
March 2021

Clinical and biochemical effect of laser as an adjunct to non-surgical treatment of chronic periodontitis.

Oral Dis 2021 Mar 13. Epub 2021 Mar 13.

Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Beijing, China.

Objectives: To evaluate the clinical and biochemical efficacy of laser therapy as an adjunct to non-surgical treatment in chronic periodontitis.

Methods: A systematic search was performed through the PubMed, EMBASE, and Cochrane Library for eligible articles published as of May 2, 2020, supplemented by information search in the System for Information on Programme Literature in Europe and a manual literature search. Only randomized controlled trials (RCTs) used to compare the adjunctive use of laser and non-surgical treatment alone with an observation period of at least 6 months were included.

Results: Sixteen RCTs with a total of 525 subjects were included. Meta-analysis suggested that the additional use of laser to scaling and root planing (SRP) showed significant superiority over SRP alone among most of clinical parameters involved. Regarding the GCF, although volume in the laser group was lower at week 4 and 12, no significant difference was found regarding the cytokines level. Subgroup analysis revealed that the combined therapy produced no significant difference in PD, CAL and PI at most time points for studies in respect to smokers. No treatment-related adverse events had been reported in the included studies.

Conclusions: Pooled analysis suggested that laser-assisted non-surgical treatment improved clinical outcome to SRP alone in the management of non-smoking chronic periodontitis patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/odi.13847DOI Listing
March 2021

Associations between weather conditions and physical activity and sedentary time in children and adolescents: A systematic review and meta-analysis.

Health Place 2021 May 10;69:102546. Epub 2021 Mar 10.

Department of Sports Science and Physical Education, The Chinese University of Hong Kong, Hong Kong, China. Electronic address:

This systematic review and meta-analysis aimed to investigate the associations between weather conditions and physical activity (PA) and sedentary time (ST) in children and adolescents. A systematic search of six databases identified 11,816 articles, of which 26 studies were included in the review. Several weather conditions were included, such as rainfall, temperature, wind speed, day length, sunlight, humidity, snowfall, and clouds. Meta-analyses showed that higher temperatures were associated with more moderate-to-vigorous PA, while lower temperature and heavily rainfall was associated with longer ST. Children and adolescents showed more PA and less ST during favorable weather conditions. Future longitudinal and intervention studies on PA and ST should focus on how weather conditions promote or hinder these behaviors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.healthplace.2021.102546DOI Listing
May 2021

Screening candidate genes related to volatile synthesis in shiitake mushrooms and construction of regulatory networks to effectively improve mushroom aroma.

J Sci Food Agric 2021 Mar 11. Epub 2021 Mar 11.

Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, People's Republic of China, Shanghai Guosen Bio-tech Co. Ltd, 1000 Jinqi Road, Shanghai, China.

Background: Metabolite formation is a physiological stress response during the growth and development of shiitake mushrooms (Lentinula edodes). The characteristic flavor metabolites are important quality components in shiitake mushrooms. To investigate the formation mechanisms of characteristic flavor metabolites, transcriptome analyses were performed on shiitake mushrooms harvested at different growth stages.

Results: In total, 30 genes related to the synthesis of characteristic volatiles of mushrooms were identified via screening. Through KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis of the selected genes and correlation analyses of gene expressions, the main volatile synthesis pathways were determined as histidine metabolism, glutathione metabolism and biosynthesis of unsaturated fatty acids. Gene cluster and correlation analyses were performed to clarify the combined effects of different genes in the enzymatic reactions. Further, a correlation network of candidate genes was built based on the gene expression levels.

Conclusion: The activities of flavor synthases and the content of characteristic flavor metabolites were analyzed; the enzyme activity changes and metabolic product distribution sites were clarified. A synthesis and regulation network was constructed for the candidate genes and characteristic volatiles, and information was obtained for 16 hub genes. Moreover, it was essential to identify and characterize the key genes and synthases involved in the synthesis of the characteristic volatiles of mushrooms. This information provides us with a better understanding of the biosynthesis and regulation of the volatiles, which will lay the foundation for improving the quality of shiitake mushrooms. © 2021 Society of Chemical Industry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jsfa.11213DOI Listing
March 2021

Artificial intelligence for breast ultrasound: An adjunct tool to reduce excessive lesion biopsy.

Eur J Radiol 2021 May 4;138:109624. Epub 2021 Mar 4.

Department of Ultrasound, Peking University Third Hospital, Beijing 10091, China.

Purpose: To determine whether adding an artificial intelligence (AI) system to breast ultrasound (US) can reduce unnecessary biopsies.

Methods: Conventional US and AI analyses were prospectively performed on 173 suspicious breast lesions before US-guided core needle biopsy or vacuum-assisted excision. Conventional US images were retrospectively reviewed according to the BI-RADS 2013 lexicon and categories. Two downgrading stratifications based on AI assessments were manually used to downgrade the BI-RADS category 4A lesions to category 3. Stratification A was used to downgrade if the assessments of both orthogonal sections of a lesion from AI were possibly benign. Stratification B was used to downgrade if the assessment of any of the orthogonal sections was possibly benign. The effects of AI-based diagnosis on lesions to reduce unnecessary biopsy were analyzed using histopathological results as reference standards.

Results: Forty-three lesions diagnosed as BI-RADS category 4A by conventional US received AI-based hypothetical downgrading. While downgrading with stratification A, 14 biopsies were correctly avoided. The biopsy rate for BI-RADS category 4A lesions decreased from 100 % to 67.4 % (P <  0.001). While downgrading with stratification B, 27 biopsies could be avoided with two malignancies missed, and the biopsy rate would decrease to 37.2 % (P <  0.05, compared with conventional US and stratification A).

Conclusion: Adding an AI system to breast US could reduce unnecessary lesion biopsies. Downgrading stratification A was recommended for its lower misdiagnosis rate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejrad.2021.109624DOI Listing
May 2021

Tolerable upper intake level of iron damages the liver of weaned piglets.

J Anim Physiol Anim Nutr (Berl) 2021 Mar 8. Epub 2021 Mar 8.

College of Animal Sciences, Zhejiang University, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou, China.

Iron is one of the essential trace elements, which is often supplemented as an additive to meet the growing needs of toddlers and young animals. Recommended nutrient intake (RNI) and tolerable upper intake levels (UL) are always set when the iron is supplemented. The purpose of this study was to evaluate the subacute (28 days) toxicity of UL iron to weaned piglet liver. Thirty 23-day-old weaned piglets were divided into three groups and, respectively, supplemented with 100, 300 or 3000 (UL) mg/kg iron. UL iron caused significant weight loss in 4th week (p < 0.05). Divalent metal transporter 1(DMT1) decreased significantly, ferroportin 1 and ferritin increased significantly in the liver of UL iron group (p < 0.05). Although there was no significant effect on liver morphology, UL iron significantly increased hepatic iron, reactive oxygen species (ROS), malondialdehyde (MDA) and protein carbonyl (p < 0.05). UL iron significantly reduced glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) and total anti-oxidation capacity (T-AOC) in the liver (p < 0.05). Nuclear factor erythroid 2-related factor 2 (Nrf2) activated subunits of glutamate cysteine ligase (Gclc) and glutathione S-transferase A1 (Gsta1) upregulation in the UL iron group liver, thereby increasing resistance to oxidative stress. In conclusion, UL iron supplementation altered iron metabolism, generated free radicals, reduced antioxidant enzyme activity and activated Nrf2 signalling pathway in the weaned piglet liver.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jpn.13521DOI Listing
March 2021

Production of HS with a Novel Short-Process for the Removal of Heavy Metals in Acidic Effluents from Smelting Flue-Gas Scrubbing Systems.

Environ Sci Technol 2021 03 5;55(6):3988-3995. Epub 2021 Mar 5.

School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.

Direct sulfidation using a high concentration of HS (HC-HS) has shown potential for heavy metals removal in various acidic effluents. However, the lack of a smooth method for producing HC-HS is a critical challenge. Herein, a novel short-process hydrolysis method was developed for the on-site production of HC-HS. Near-perfect 100% efficiency and selectivity were obtained via CS hydrolysis over the ZrO-based catalyst. Meanwhile, no apparent residual sulfur/sulfate poisoning was detected, which guaranteed long-term operation. The coexistence of CO in the products had a negligible effect on the complete hydrolysis of CS. HS production followed a sequential hydrolysis pathway, with the reactions for CS adsorption and dissociation being the rate-determining steps. The energy balance indicated that HC-HS production was a mildly exothermic reaction, and the heat energy could be maintained at self-balance with approximately 80% heat recovery. The batch sulfidation efficiencies for As(III), Hg(II), Pb(II), and Cd(II) removal were over 99.9%, following the solubilities () of the corresponding metal sulfides. CO in the mixed gas produced by CS hydrolysis did not affect heavy metals sulfidation due to the presence of abundant H. Finally, a pilot-scale experiment successfully demonstrated the practical effects. Therefore, this novel on-site HC-HS production method adequately achieved heavy metals removal requirements in acidic effluents.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.0c07884DOI Listing
March 2021