Publications by authors named "Jie Dong"

722 Publications

Prevalence of hypertension and hypertension phenotypes after three visits in Chinese urban children.

J Hypertens 2021 Jul 16. Epub 2021 Jul 16.

Pediatrics Research Institute of Hunan Province, Hunan Children's Hospital, Changsha Department of Non-communicable Disease Management, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health Capital Institute of Pediatrics, Beijing, China.

Objective: This study aims to evaluate the prevalence of hypertension and hypertension subtypes among Chinese children aged 6--17 years in a multicenter school-based sample by three separate screenings.

Methods: Students from six major cities in China (Changchun, Beijing, Jinan, Shanghai, Chongqing, and Chengdu) were recruited in this cross-sectional survey during 2012 and 2015. Each participant was seated and had three consecutive blood pressure measurements on the right arm in the morning by an automated oscillometric device and the hypertensive ones were followed to the next visit. Hypertension was diagnosed by BP references for Chinese children and adolescents in 2010.

Results: Data from 44 396 children aged 6--17 years were included in analysis, 50.9% of whom were boys. The prevalence of confirmed hypertension after three separate screenings was 4% in the total population, 5% in boys, and 3% in girls, respectively. The prevalence of confirmed isolated systolic hypertension (ISH), isolated diastolic hypertension (IDH), and systolic and diastolic hypertension (SDH) in the total population was 2.7, 0.3 and 1%, respectively.

Conclusion: Around 4% urban Chinese children and adolescents aged 6-17 years were hypertensive after three separate BP screenings in 2012-2015. ISH was the most frequent form of hypertension in children.
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http://dx.doi.org/10.1097/HJH.0000000000002977DOI Listing
July 2021

Characteristics and Related Factors of High-Risk Human Papillomavirus Infection in Pregnant Women.

Med Sci Monit 2021 Jul 12;27:e929100. Epub 2021 Jul 12.

Department of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China (mainland).

BACKGROUND Cervical cancer is a risk for women worldwide. The aim of this study was to examine the occurrence of high-risk human papillomavirus (HR-HPV) infection and its related factors in pregnant women and provide a scientific basis for the targeted prevention and treatment of cervical cancer in pregnant women. MATERIAL AND METHODS A total of 1774 pregnant women were included, and 1774 non-pregnant women were selected as controls. Cervical exfoliated cells were collected from all women for HR-HPV (AptimaE6, E7mRNA) and ThinPrep cytologic testing, and the vaginal discharge of all pregnant women was tested for pH level and routine pathogenic microorganisms. RESULTS The HPV-16-positive and HPV-16/18/45-positive rates in pregnant women were higher than those of non-pregnant women (P<0.05). There was a statistically significant difference in HR-HPV-positive rate, HPV-16-positive rate, and non-HPV-16/18/45-positive rate among pregnant women of different ages (P<0.05). There was a statistically significant difference in HR-HPV-positive rate and non-HPV-16/18/45-positive rate in the first, second, and third trimester (P<0.05). The HR-HPV-positive rate, HPV-16-positive rate, HPV-18/45-positive rate, and non-HPV-16/18/45-positive rate of pregnant women with concurrent infection were higher than those in women without concurrent infection (P<0.05). The HR-HPV-positive and HPV-16/18/45-positive rates in pregnant women were associated with cytologic examination results (P<0.05). CONCLUSIONS The overall infection rates of HR-HPV-16 and HR-HPV-18/45 in pregnant women were higher than those in non-pregnant women. The gestation period was found to be a susceptible period for infection with HR-HPV, and we recommend the implementation of cervical cancer screening based on HR-HPV testing in pregnant women.
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http://dx.doi.org/10.12659/MSM.929100DOI Listing
July 2021

Feline Stool-Associated Circular DNA Virus (FeSCV) in Diarrheic Cats in China.

Front Vet Sci 2021 16;8:694089. Epub 2021 Jun 16.

College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

Feline stool-associated circular DNA virus (FeSCV) is an unclassified circular replication-associated protein-encoding single-stranded (CRESS) DNA virus that was discovered in cats in Japan in 2018. Few studies on the genomic characteristics and prevalence of FeSCV have been conducted. To investigate whether FeSCV has been circulating in domestic cats in Guangdong, China, fecal samples were collected from cats with diarrhea in an animal hospital in 2018 to promote research on FeSCV. The FeSCV genome was obtained by PCR amplification and sequencing, and the detected virus was named PY4 (GenBank No. MT732515). The genome of PY4 was 2,034 nt in size, which was 12 nt smaller than the reported genome of Japanese FeSCV strains (KU7, KU8, KU9, KU14) (2,046 nt). The PY4 strain shared 95.1 ~ 95.5% homology with Japanese FeSCV strains. Notably, the Cap protein of PY4 was mutated at 15 amino acid sites, and the PY4 genome contained a unique open reading frame 3. In addition, there were two additional base insertions in the stem-loop structure of PY4, and the nucleotide homology of the spacer region was not high. A phylogenetic tree based on Rep proteins showed that PY4, Japanese FeSCVs and rodent stool-associated circular viruses (RodSCVs) clustered together, suggesting that they might share a similar origin in their phylogenetic evolution. In this study, samples collected in Guangzhou, China, in 2018 were subjected to an etiological investigation, and 20% (2/10) of the samples were positive for FeSCV. The ORFs, stem-loop structures, Cap proteins and intergenic region sequences of PY4 were significantly different from those reported in Japan. This is the first report of FeSCV in domestic cats with diarrhea in China, and further epidemiological studies are urgently needed to assess the impact of the virus on cats.
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http://dx.doi.org/10.3389/fvets.2021.694089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242157PMC
June 2021

Scope and heterogeneity of outcomes reported in randomized trials in patients receiving peritoneal dialysis.

Clin Kidney J 2021 Jul 31;14(7):1817-1825. Epub 2020 Dec 31.

Sydney School of Public Health, University of Sydney, Sydney, NSW, Australia.

Background: Randomized trials can provide evidence to inform decision-making but this may be limited if the outcomes of importance to patients and clinicians are omitted or reported inconsistently. We aimed to assess the scope and heterogeneity of outcomes reported in trials in peritoneal dialysis (PD).

Methods: We searched the Cochrane Kidney and Transplant Specialized Register for randomized trials in PD. We extracted all reported outcome domains and measurements and analyzed their frequency and characteristics.

Results: From 128 reports of 120 included trials, 80 different outcome domains were reported. Overall, 39 (49%) domains were surrogate, 23 (29%) patient-reported and 18 (22%) clinical. The five most commonly reported domains were PD-related infection [59 (49%) trials], dialysis solute clearance [51 (42%)], kidney function [45 (38%)], protein metabolism [44 (37%)] and inflammatory markers/oxidative stress [42 (35%)]. Quality of life was reported infrequently (4% of trials). Only 14 (12%) trials included a patient-reported outcome as a primary outcome. The median number of outcome measures (defined as a different measurement, aggregation and metric) was 22 (interquartile range 13-37) per trial. PD-related infection was the most frequently reported clinical outcome as well as the most frequently stated primary outcome. A total of 383 different measures for infection were used, with 66 used more than once.

Conclusions: Trials in PD include important clinical outcomes such as infection, but these are measured and reported inconsistently. Patient-reported outcomes are infrequently reported and nearly half of the domains were surrogate. Standardized outcomes for PD trials are required to improve efficiency and relevance.
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http://dx.doi.org/10.1093/ckj/sfaa224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243273PMC
July 2021

Identification of long noncoding RNAs reveals the effects of dinotefuran on the brain in Apis mellifera (Hymenopptera: Apidae).

BMC Genomics 2021 Jul 3;22(1):502. Epub 2021 Jul 3.

Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

Background: Dinotefuran (CAS No. 165252-70-0), a neonicotinoid insecticide, has been used to protect various crops against invertebrate pests and has been associated with numerous negative sublethal effects on honey bees. Long noncoding RNAs (lncRNAs) play important roles in mediating various biological and pathological processes, involving transcriptional and gene regulation. The effects of dinotefuran on lncRNA expression and lncRNA function in the honey bee brain are still obscure.

Results: Through RNA sequencing, a comprehensive analysis of lncRNAs and mRNAs was performed following exposure to 0.01 mg/L dinotefuran for 1, 5, and 10 d. In total, 312 lncRNAs and 1341 mRNAs, 347 lncRNAs and 1458 mRNAs, and 345 lncRNAs and 1155 mRNAs were found to be differentially expressed (DE) on days 1, 5 and 10, respectively. Gene set enrichment analysis (GSEA) indicated that the dinotefuran-treated group showed enrichment in carbohydrate and protein metabolism and immune-inflammatory responses such as glycine, serine and threonine metabolism, pentose and glucuronate interconversion, and Hippo and transforming growth factor-β (TGF-β) signaling pathways. Moreover, the DE lncRNA TCONS_00086519 was shown by fluorescence in situ hybridization (FISH) to be distributed mainly in the cytoplasm, suggesting that it may serve as a competing endogenous RNA and a regulatory factor in the immune response to dinotefuran.

Conclusion: This study characterized the expression profile of lncRNAs upon exposure to neonicotinoid insecticides in young adult honey bees and provided a framework for further study of the role of lncRNAs in honey bee growth and the immune response.
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http://dx.doi.org/10.1186/s12864-021-07811-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254963PMC
July 2021

Long noncoding RNA lncRNA354 functions as a competing endogenous RNA of miR160b to regulate ARF genes in response to salt stress in upland cotton.

Plant Cell Environ 2021 Jun 24. Epub 2021 Jun 24.

State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an, Shandong, China.

Long non-coding RNAs (lncRNAs) play important roles in response to biotic and abiotic stress through acting as competing endogenous RNAs (ceRNAs) to decoy mature miRNAs. However, whether this mechanism is involved in cotton salt stress response remains unknown. We report the characterization of an endogenous lncRNA, lncRNA354, whose expression was reduced in salt-treated cotton and was localized at the nucleus and cytoplasm. Using endogenous target mimic (eTM) analysis, we predicted that lncRNA354 had a potential binding site for miR160b. Transient expression in tobacco demonstrated that lncRNA354 was a miR160b eTM and attenuated miR160b suppression of its target genes including auxin response factors (ARFs). Silencing or overexpressing lncRNA354 affected the expression of miR160b and target ARFs. Silencing lncRNA354 and targets GhARF17/18 resulted in taller cotton plants and enhanced the resistant to salt stress. Overexpression of lncRNA354 and targets GhARF17/18 in Arabidopsis led to dwarf plants, decreased root dry weight, and reduced salt tolerance. Our results show that the lncRNA354-miR160b effect on GhARF17/18 expression may modulate auxin signaling and thus affect growth. These results also shed new light on a mechanism of lncRNA-associated responses to salt stress. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/pce.14133DOI Listing
June 2021

Cefoperazone and sulbactam-related eosinophilic peritonitis: a case report and literature review.

J Int Med Res 2021 Jun;49(6):3000605211025367

Renal Division, Department of Medicine, Peking University First Hospital, Beijing, People's Republic of China.

Eosinophilic peritonitis (EP) is a well-described complication of peritoneal dialysis that occurs because of an overreaction to constituents that are related to the catheter or tubing, peritoneal dialysate, pathogenic infection, or intraperitoneal drug use. EP caused by antibiotic use is rare. We present the case of a patient with cefoperazone and sulbactam-related EP. A 59-year-old woman who was undergoing peritoneal dialysis presented with peritonitis with abdominal pain and turbid peritoneal dialysis. Empiric intraperitoneal cefazolin in combination with cefoperazone and sulbactam was started after peritoneal dialysis effluent cultures were performed. Her peritonitis achieved remission in 2 days with the help of cephalosporin, but she developed EP 1 week later, when her dialysate eosinophil count peaked at 49% of the total dialysate white blood cells (absolute count, 110/mm). We excluded other possible causes and speculated that cefoperazone and sulbactam was the probable cause of EP. The patient continued treatment with cefoperazone and sulbactam for 14 days. EP resolved within 48 hours after stopping cefoperazone and sulbactam. Thus, EP can be caused by cefoperazone and sulbactam use. Physicians should be able to distinguish antibiotic-related EP from refractory peritonitis to avoid technique failure.
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http://dx.doi.org/10.1177/03000605211025367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236785PMC
June 2021

Pretreatment systemic inflammation response index is predictive of pathological complete response in patients with breast cancer receiving neoadjuvant chemotherapy.

BMC Cancer 2021 Jun 14;21(1):700. Epub 2021 Jun 14.

Department of Medical Oncology, Anhui Provincial Hospital affiliated to Anhui Medical University, Hefei, 2300001, Anhui Province, China.

Background: Inflammation plays an important role in tumor proliferation, metastasis, and resistance to chemotherapy. The systemic inflammation response index (SIRI), has been reported to be closely related to prognosis in many tumors, such as breast and gastric cancers. However, the predictive value of pretreatment SIRI on pathological complete response (pCR) rates in patients with breast cancer treated with neoadjuvant chemotherapy (NAC) is unknown. This study examined the correlation between SIRI and pCR in patients with breast cancer receiving NAC and identified convenient and accurate predictive indicators for pCR.

Methods: We retrospectively analyzed the clinicopathological parameters and pretreatment peripheral blood characteristics of the 241 patients with breast cancer who received NAC between June 2015 and June 2020. Receiver operating characteristic (ROC) curves were used to determine the optimal cutoff of SIRI. ROC curves were also plotted to verify the accuracy of inflammatory markers for pCR prediction. The chi-squared test was used to explore the relationships of SIRI with pCR and other clinicopathological parameters. Multivariate analyses were performed using a logistic regression model.

Results: Among the 241 patients, 48 (19.92%) achieved pCR. pCR was significantly related to SIRI, the neutrophil-lymphocyte ratio (NLR), the lymphocyte-monocyte ratio (LMR), molecular subtypes and other clinicopathological parameters, such as BMI, clinical T and N staging, and histological grade. Multivariate analyses indicated that the clinical T and N staging, SIRI, and NLR were independent prognostic factors for pCR in patients with breast cancer. The area under the ROC curve for SIRI was larger than that for NLR. Compared to patients with SIRI ≥0.72, patients with SIRI < 0.72 had a nearly 5-fold higher chance of obtaining pCR (odds ratio = 4.999, 95% confidence interval = 1.510-16.551, p = 0.000).

Conclusions: Pretreatment SIRI is predictive of pCR in patients with breast cancer receiving NAC, and the index can assist physicians in formulating personalized treatment strategies.
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http://dx.doi.org/10.1186/s12885-021-08458-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204500PMC
June 2021

The Association between Baseline Serum Lipids and Mortality in Peritoneal Dialysis Patients.

Blood Purif 2021 Jun 11:1-10. Epub 2021 Jun 11.

Division of Nephrology, Huashan Hospital, Fudan University, Shanghai, China.

Introduction: Lipid disturbances are common in ESRD patients. In peritoneal dialysis (PD) patients, dyslipidemia is even more common. This study aimed to examine whether serum lipids were associated with prognosis of PD patients.

Methods: Patients from a multicenter retrospective cohort were used for the present study. The primary endpoint was all-cause mortality. Cox regression was used to analyze the association between serum lipids including total cholesterol, low-density lipoprotein (LDL), high-density lipoprotein, and triglycerides and the prognosis.

Results: The results showed that lower total cholesterol and LDL levels at the initiation of PD predicted higher all-cause mortality in PD patients. Multivariate analysis reveal that the association disappeared after adjusting for age, gender, albumin, prealbumin, protein catabolic rate normalized to body weight, C-reactive protein, and residual renal function. Further analysis showed that patients with lower total cholesterol/LDL had a higher mortality only during the first 24 months of follow-up. In the patients who survived >2 years after PD, lower total cholesterol/LDL was not associated with higher long-term all-cause mortality any more.

Conclusion: Lower total cholesterol/LDL levels at the initiation of PD were associated with overall mortality in PD patients. The association could be potentially modified by malnutrition, inflammation, and residual renal function or disappeared after 24 months.
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http://dx.doi.org/10.1159/000513945DOI Listing
June 2021

Dual-Selective Catalysis in Dephosphorylation Tuned by Hf-Containing Metal-Organic Frameworks Mimicking Phosphatase.

ACS Cent Sci 2021 May 7;7(5):831-840. Epub 2021 May 7.

Key Laboratory of Advanced Energy Material Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.

Selective dephosphorylation is full of great challenges in the field of biomimetic catalysis. To mimic the active sites of protein phosphatase, Hf-OH-Hf motif-containing metal-organic frameworks (MOFs) were obtained and structurally characterized, which are assembled from [HfNi] cubic nanocages and exhibit good stability in various solvents and acid/base solutions. Catalytic investigations suggest as-synthesized and display accurate type-selectivity (selectively catalyzed P-O rather than S-O or C-O bonds) and position-selectivity (selectively catalyzed phosphomonoesters over phosphodiesters) for the hydrolysis of phosphoesters. Reaction kinetic studies further revealed the high activity of the catalytic sites in these catalysts, and the unique catalytic selectivity and high activity are comparable to phosphatase. Additionally, these MOF catalysts possess good recursivity and hypotoxicity. Control experiments (including Hf- and Zr-based isomorphous MOFs) and theoretical calculations indicate that both triplet nickel and Hf clusters play significant roles in the unique binding site and favorable binding energy. To our knowledge, this is the first example of selective dephosphorylation through MOF catalysts as mimic enzymes, which paves a potential way for the development of specific therapeutic MOFs.
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http://dx.doi.org/10.1021/acscentsci.0c01581DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161481PMC
May 2021

Mixed reality models based on low-dose computed tomography technology in nephron-sparing surgery are better than models based on normal-dose computed tomography.

Quant Imaging Med Surg 2021 Jun;11(6):2658-2668

Department of Medical Imaging, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.

Background: Nephron-sparing surgery has been widely applied in the treatment of renal tumors. Previous studies have confirmed the advantages of mixed reality technology in surgery. The study aimed to explore the optimization of mixed reality technology and its application value in nephron-sparing surgery.

Methods: In this prospective study of 150 patients with complex renal tumors (RENAL nephrometry score ≥7) who underwent nephron-sparing surgery, patients were randomly divided into Group A (the normal-dose mixed reality group, n=50), Group B (the low-dose mixed reality group, n=50), and Group C (the traditional computed tomography image group, n=50). Group A and Group C received the normal-dose computed tomography scan protocol: 120 kVp, 400 mA, and 350 mgI/mL, while Group B received the low-dose computed tomography scan protocol: 80 kVp, automatic tube current modulation, and 320 mgI/mL. All computed tomography data were transmitted to a three-dimensional visualization workstation and underwent modeling and mixed reality imaging. Two senior surgeons evaluated mixed reality quality. Objective indexes and perioperative indexes were calculated and compared.

Results: Compared with Group A, the radiation effective dose in Group B was decreased by 39.6%. The subjective scores of mixed reality quality in Group B were significantly higher than those of Group A (Z=-4.186, P<0.001). The inter-observer agreement between the two senior surgeons in mixed reality quality was excellent (K=0.840, P<0.001). The perioperative indexes showed that the mixed reality groups were significantly different from the computed tomography image group (all P<0.017). More cases underwent nephron-sparing surgery in the mixed reality groups than in the computed tomography image group (P<0.0017).

Conclusions: Low-dose computed tomography technology can be effectively applied to mixed reality optimization, reducing the effective dose and improving mixed reality quality. Optimized mixed reality can significantly increase the cases of successful nephron-sparing surgery and improve perioperative indexes.
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http://dx.doi.org/10.21037/qims-20-956DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107318PMC
June 2021

Distribution Characteristics, Pollution Assessment, and Source Identification of Heavy Metals in Soils Around a Landfill-Farmland Multisource Hybrid District.

Arch Environ Contam Toxicol 2021 Jul 31;81(1):77-90. Epub 2021 May 31.

Qingdao Geo-Engineering Surveying Institute (Qingdao Geological Exploration and Development Bureau); Key Laboratory of Urban Geology and Underground Space Resources; Shandong Provincial Bureau of Geology and Mineral Resources, Qingdao Geology and Geotechnical Engineering Co., Ltd, Qingdao, 266100, China.

Heavy-metal pollution is a negative impact of municipal solid-waste landfills. The multiple pollution transport pathways (including leachate, runoff, and waste gas) and complex and co-existing potential pollution sources (such as agricultural activities) around landfills require a combination of different pollution assessment methods and source identification tools to address pollution distribution and potential risks. In this study, the distributions of eight heavy metals (chromium (Cr), copper (Cu), nickel (Ni), lead (Pb), zinc (Zn), arsenic (As), cadmium (Cd), and mercury (Hg)) around a landfill were analyzed using 60 topsoil samples. Ecological risk assessments indicated that there are currently no ecological risks. Based on health risk assessments, however, high concentrations of Cr and As in the soil pose a noncarcinogenic and carcinogenic risk to humans in the study area, respectively. In addition, the geoaccumulation indices for Cr, Cu, Ni, Zn, As, and Hg confirmed anthropogenic sources of accumulation of these metals in soils. Additionally, the potential ecological risk index indicated that Hg posed a considerable risk to the ecology of the area around the landfill. Sources of heavy metals in the study area were attributed to natural sources (22.10%), agricultural activities (27.65%), landfill (31.35%), and transportation (18.89%). The continuous accumulation of heavy metals and health risk for humans suggests the need to continuously monitor of heavy metal content and migration around the landfill. This study provides a reference for local authorities in the study area.
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http://dx.doi.org/10.1007/s00244-021-00857-9DOI Listing
July 2021

Novel biallelic mutations in MEI1: expanding the phenotypic spectrum to human embryonic arrest and recurrent implantation failure.

Hum Reprod 2021 Jul;36(8):2371-2381

Institute of Pediatrics, Children's Hospital of Fudan University and Institutes of Biomedical Sciences, State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, China.

Study Question: Are any novel mutations and corresponding new phenotypes, other than recurrent hydatidiform moles, seen in patients with MEI1 mutations?

Summary Answer: We identified several novel mutations in MEI1 causing new phenotypes of early embryonic arrest and recurrent implantation failure.

What Is Known Already: It has been reported that biallelic mutations in MEI1, encoding meiotic double-stranded break formation protein 1, cause azoospermia in men and recurrent hydatidiform moles in women.

Study Design, Size, Duration: We first focused on a pedigree in which two sisters were diagnosed with recurrent hydatidiform moles in December 2018. After genetic analysis, two novel mutations in MEI1 were identified. We then expanded the mutational screening to patients with the phenotype of embryonic arrest, recurrent implantation failure, and recurrent pregnancy loss, and found another three novel MEI1 mutations in seven new patients from six families recruited from December 2018 to May 2020.

Participants/materials, Setting, Methods: Nine primary infertility patients were recruited from the reproduction centers in local hospitals. Genomic DNA from the affected individuals, their family members, and healthy controls was extracted from peripheral blood. The MEI1 mutations were screened using whole-exome sequencing and were confirmed by the Sanger sequencing. In silico analysis of mutations was performed with Sorting Intolerant From Tolerant (SIFT) and Protein Variation Effect Analyzer (PROVEAN). The influence of the MEI1 mutations was determined by western blotting and minigene analysis in vitro.

Main Results And The Role Of Chance: In this study, we identified five novel mutations in MEI1 in nine patients from seven independent families. Apart from recurrent hydatidiform moles, biallelic mutations in MEI1 were also associated with early embryonic arrest and recurrent implantation failure. In addition, we demonstrated that protein-truncating and missense mutations reduced the protein level of MEI1, while the splicing mutations caused abnormal alternative splicing of MEI1.

Limitations, Reasons For Caution: Owing to the lack of in vivo data from the oocytes of the patients, the exact molecular mechanism(s) involved in the phenotypes remains unknown and should be further investigated using knock-out or knock-in mice.

Wider Implications Of The Findings: Our results not only reveal the important role of MEI1 in human oocyte meiosis and early embryonic development, but also extend the phenotypic and mutational spectrum of MEI1 and provide new diagnostic markers for genetic counseling of clinical patients.

Study Funding/competing Interest(s): This work was supported by the National Key Research and Development Program of China (2018YFC1003800, 2017YFC1001500, and 2016YFC1000600), the National Natural Science Foundation of China (81725006, 81822019, 81771581, 81971450, and 81971382), the project supported by the Shanghai Municipal Science and Technology Major Project (2017SHZDZX01), the Project of the Shanghai Municipal Science and Technology Commission (19JC1411001), the Natural Science Foundation of Shanghai (19ZR1444500), the Shuguang Program of the Shanghai Education Development Foundation and the Shanghai Municipal Education Commission (18SG03), the Shanghai Health and Family Planning Commission Foundation (20154Y0162), the Strategic Collaborative Research Program of the Ferring Institute of Reproductive Medicine, Ferring Pharmaceuticals and the Chinese Academy of Sciences (FIRMC200507) and the Chongqing Key Laboratory of Human Embryo Engineering (2020KFKT008). No competing interests are declared.

Trial Registration Number: N/A.
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http://dx.doi.org/10.1093/humrep/deab118DOI Listing
July 2021

QPCT regulation by CTCF leads to sunitinib resistance in renal cell carcinoma by promoting angiogenesis.

Int J Oncol 2021 07 26;59(1). Epub 2021 May 26.

Department of Urology, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210002, P.R. China.

Sunitinib is widely used as a first‑line treatment for advanced renal cell carcinoma (RCC). However, a number of patients with RCC who receive sunitinib develop drug resistance; and the biological mechanisms involved in resistance to sunitinib remain unclear. It has previously been suggested that the protein glutaminyl‑peptide cyclotransferase (QPCT) is closely related to sunitinib resistance in RCC. Thus, in the present study, in order to further examine the molecular mechanisms responsible for sunitinib resistance in RCC, sunitinib‑non‑responsive and ‑responsive RCC tissue and plasma samples were collected and additional experiments were performed in order to elucidate the molecular mechanisms responsible for sunitinib resistance in RCC. The upstream and downstream regulatory mechanisms of QPCT were also evaluated. On the whole, the data from the present study suggest that QPCT, CCCTC‑binding factor (CTCF) and phosphatidylinositol‑4,5‑bisphosphate 3‑kinase catalytic subunit alpha (PIK3CA) may be used as targets for predicting, reversing and treating sunitinib‑resistant RCC.
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http://dx.doi.org/10.3892/ijo.2021.5228DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208629PMC
July 2021

Peripheral Injection of Tim-3 Antibody Attenuates VSV Encephalitis by Enhancing MHC-I Presentation.

Front Immunol 2021 7;12:667478. Epub 2021 May 7.

Department of Neuroimmune and Antibody Engineering, Beijing Institute of Basic Medical Sciences, Beijing, China.

Viral encephalitis is the most common cause of encephalitis. It is responsible for high morbidity rates, permanent neurological sequelae, and even high mortality rates. The host immune response plays a critical role in preventing or clearing invading pathogens, especially when effective antiviral treatment is lacking. However, due to blockade of the blood-brain barrier, it remains unclear how peripheral immune cells contribute to the fight against intracerebral viruses. Here, we report that peripheral injection of an antibody against human Tim-3, an immune checkpoint inhibitor widely expressed on immune cells, markedly attenuated vesicular stomatitis virus (VSV) encephalitis, marked by decreased mortality and improved neuroethology in mice. Peripheral injection of Tim-3 antibody enhanced the recruitment of immune cells to the brain, increased the expression of major histocompatibility complex-I (MHC-I) on macrophages, and as a result, promoted the activation of VSV-specific CD8 T cells. Depletion of macrophages abolished the peripheral injection-mediated protection against VSV encephalitis. Notably, for the first time, we found a novel post-translational modification of MHC-I by Tim-3, wherein, by enhancing the expression of MARCH9, Tim-3 promoted the proteasome-dependent degradation of MHC-I K48-linked ubiquitination in macrophages. These results provide insights into the immune response against intracranial infections; thus, manipulating the peripheral immune cells with Tim-3 antibody to fight viruses in the brain may have potential applications for combating viral encephalitis.
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http://dx.doi.org/10.3389/fimmu.2021.667478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138436PMC
May 2021

Magnetic resonance imaging reconstruction algorithm under complex convolutional neural network in diagnosis and prognosis of cerebral infarction.

PLoS One 2021 17;16(5):e0251529. Epub 2021 May 17.

School of Physics and Microelectronics, Zhengzhou University, Zhengzhou, P.R. China.

This study was to explore the application value of magnetic resonance imaging (MRI) image reconstruction model based on complex convolutional neural network (CCNN) in the diagnosis and prognosis of cerebral infarction. Two image reconstruction methods, frequency domain reconstruction network (FDRN) and image domain reconstruction network (IDRN), were introduced based on the CCNN algorithm. In addition, they were integrated to form two new MRI image reconstruction models, namely D-FDRN and D-IDRN. The peak signal to noise ratio (PSNR) value and structural similarity index measure (SSIM) value of the image were compared and analyzed before and after the integration. The MRI images of patients with cerebral infarction in the dataset were undertaken as the data source, the average diffusion coefficient (DCavg) and apparent diffusion coefficient (ADC) values of different parts of the MRI image were measured, respectively. The correlation of the vein abnormality grading (VABG) to the infarct size and the degree of stenosis of the responsible vessel was analyzed in this study. The results showed that the PSNR and SSIM values of the MRI reconstructed image of the D-IDRN algorithm based on the CCNN algorithm in this study were higher than those of other algorithms. There was a positive correlation between the VABG and the infarct size (r = 0.48 and P = 0.002), and there was a positive correlation between the VABG the degree of stenosis of the responsible vessel (r = 0.58 and P < 0.0001). The ADC value of the central area of the infarct on the affected side was significantly greatly lower than that of the normal side (P < 0.01), and the DCavg value of the central area of the infarct was much lower in contrast to the normal side (P < 0.05). It indicated that an image reconstruction algorithm constructed in this study could improve the quality of MRI images. The ADC value and DCavg value changed in the infarct central area could be used as the basis for the diagnosis of cerebral infarction. If the vein was abnormal, the patient suffered from severe vascular stenosis, large infarction area, and poorer prognosis.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0251529PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128241PMC
May 2021

PharmSD: A novel AI-based computational platform for solid dispersion formulation design.

Int J Pharm 2021 Jul 13;604:120705. Epub 2021 May 13.

State Key Laboratory of Quality Research in Chinese Medicine, ICMS, University of Macau, China. Electronic address:

Solid dispersion is an effective way to improve the dissolution and oral bioavailability of water-insoluble drugs. To obtain an effective solid dispersion formulation, researchers need to evaluate a series of important properties of the designed formulation, including in vitro dissolution and physical stability of solid dispersion. It is usually time-consuming and labor-intensive to explore these properties by traditional experimental methods. However, the development of machine learning technology provides a powerful way to solve such problems. By using advanced machine learning algorithms, we established a series of robust models and finally formed a systematic strategy to assist the formulation design. Based on these works, we developed a new formulation prediction platform of solid dispersion: PharmSD. This platform provides efficient functionalities for the prediction of physical stability, dissolution type and dissolution rate of solid dispersion independently. Then, a virtual screening pipeline can be produced by considering those prediction results as a whole, which enables users to filter different kinds of drug-polymer combinations in various experimental situations and figure out which combination could form the best formulation. Moreover, it also provides two tools that enable researchers to evaluate the application domain of models and calculate the similarity of dissolution curves. PharmSD is expected to be the first freely available web-based platform that is fully designed for the formulation design of solid dispersion driven by machine learning. We hope this platform could provide a powerful solution to assist the formulation design in the related research area. It is available at: http://pharmsd.computpharm.org.
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http://dx.doi.org/10.1016/j.ijpharm.2021.120705DOI Listing
July 2021

Distribution of avian influenza viruses according to environmental surveillance during 2014-2018, China.

Infect Dis Poverty 2021 May 6;10(1):60. Epub 2021 May 6.

National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention; WHO Collaborating Center for Reference and Research On Influenza; Key Laboratory for Biosafety, National Health Commission, Beijing, China.

Background: Recurrent infections of animal hosts with avian influenza viruses (AIVs) have posted a persistent threat. It is very important to understand the avian influenza virus distribution and characteristics in environment associated with poultry and wild bird. The aim of this study was to analyze the geographic and seasonal distributions of AIVs in the 31 provinces, municipalities and autonomous region (PMA) of China, compare the AIVs prevalence in different collecting sites and sampling types, analyze the diversity of AIVs subtypes in environment.

Methods: A total of 742 005 environmental samples were collected from environmental samples related to poultry and wild birds in different locations in the mainland of China during 2014-2018. Viral RNA was extracted from the environmental samples. Real-time RT-PCR assays for influenza A, H5, H7 and H9 subtypes were performed on all the samples to identify subtypes of influenza virus. The nucleic acid of influenza A-positive samples were inoculated into embryonated chicken eggs for virus isolation. Whole-genome sequencing was then performed on Illumina platform. SPSS software was used to paired t test for the statistical analysis. ArcGIS was used for drawing map. Graphpad Prism was used to make graph.

Results: The nucleic acid positivity rate of influenza A, H5, H7 and H9 subtypes displayed the different characteristics of geographic distribution. The nucleic acid positivity rates of influenza A were particularly high (25.96%-45.51%) in eleven provinces covered the Central, Eastern, Southern, Southwest and Northwest of China. The nucleic acid positivity rates of H5 were significantly high (11.42%-13.79%) in two provinces and one municipality covered the Southwest and Central of China. The nucleic acid positivity rates of H7 were up to 4% in five provinces covered the Eastern and Central of China. The nucleic acid positivity rates of H9 were higher (13.07%-2.07%) in eleven PMA covered the Southern, Eastern, Central, Southwest and Northwest of China. The nucleic acid positivity rate of influenza A, H5, H7 and H9 showed the same seasonality. The highest nucleic acid positivity rates of influenza A, H5, H7, H9 subtypes were detected in December and January and lowest from May to September. Significant higher nucleic acid positivity rate of influenza A, H5, H7 and H9 were detected in samples collected from live poultry markets (LPM) (30.42%, 5.59%, 4.26%, 17.78%) and poultry slaughterhouses (22.96%, 4.2%, 2.08%, 12.63%). Environmental samples that were collected from sewage and chopping boards had significantly higher nucleic acid positivity rates for influenza A (36.58% and 33.1%), H5 (10.22% and 7.29%), H7(4.24% and 5.69%)and H9(21.62% and 18.75%). Multiple subtypes of AIVs including nine hemagglutinin (HA) and seven neuraminidase (NA) subtypes were isolated form the environmental samples. The H5, H7, and H9 subtypes accounted for the majority of AIVs in environment.

Conclusions: In this study, we found the avian influenza viruses characteristics of geographic distribution, seasonality, location, samples types, proved that multiple subtypes of AIVs continuously coexisted in the environment associated with poultry and wild bird, highlighted the need for environmental surveillance in China.
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http://dx.doi.org/10.1186/s40249-021-00850-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101199PMC
May 2021

The contributions of the left hippocampus and bilateral inferior parietal lobule to form-meaning associative learning.

Psychophysiology 2021 Aug 5;58(8):e13834. Epub 2021 May 5.

Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education, Guangzhou, China.

Existing studies have identified crucial roles for the hippocampus and a distributed set of cortical regions (e.g., the inferior parietal cortex) in learning novel words. Nevertheless, researchers have not clearly determined how the hippocampus and cortical regions dynamically interact during novel word learning, especially during form-meaning associative learning. As a method to address this question, we used an online learning paradigm and representational similarity analysis to explore the contributions of the hippocampus and neocortex to form-meaning associative learning. Twenty-nine native Chinese college students were recruited to learn 30 form-meaning pairs, which were repeated 7 times during fMRI scan. Form-meaning associative learning elicited activations in a wide neural network including regions required for word processing (i.e., the bilateral inferior frontal gyrus and the occipitotemporal cortex), regions required for encoding (i.e., the bilateral parahippocampus and hippocampus), and regions required for cognitive control (i.e., the anterior cingulate cortex and dorsolateral prefrontal cortex). More importantly, our study revealed the differential roles of the left hippocampus and bilateral inferior parietal lobule (IPL) in form-meaning associative learning. Specifically, higher pattern similarity in the bilateral IPL in the early learning phase (repetitions 1 to 3) was related to better learning performance, while higher pattern similarity in the left hippocampus in the late learning phase (repetitions 5 to 7) was associated with better learning performance. These findings indicate that the hippocampus and cortical regions (e.g., the IPL) contribute to form-meaning learning in different stages.
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http://dx.doi.org/10.1111/psyp.13834DOI Listing
August 2021

Identification of Novel Mutations in : Expanding the Mutational Spectrum for Female Infertility.

Front Cell Dev Biol 2021 9;9:647130. Epub 2021 Apr 9.

Institute of Pediatrics, Children's Hospital of Fudan University and Institutes of Biomedical Sciences, State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, China.

Oocyte maturation and fertilization are fundamental processes for successful human reproduction, and abnormalities in these processes will cause infertility. Recently, we identified biallelic mutations in that are responsible for human oocyte maturation arrest, fertilization failure, and early embryonic development arrest. In this study, we screened for further mutations in a new cohort of patients with abnormalities in oocyte maturation, fertilization, and early embryonic development. Through whole-exome sequencing, we identified the four novel mutations c.887G > A (p. Arg296Gln), c.964C > T (p.Arg322), c.1155G > C (p.Trp385Cys), and c.330 + 1G > A (p. Glu111Ilefs36) and one previously reported mutation c.965G > A (p.Arg322Gln) in in four infertile individuals from three independent families. The patients had different phenotypes of oocyte maturation arrest and fertilization failure resulting from the different mutations. This study confirms our previous research and expands the spectrum of known mutations in , providing new evidence supporting the function of in the genetic etiology of female infertility characterized by oocyte maturation arrest and fertilization failure.
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http://dx.doi.org/10.3389/fcell.2021.647130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063106PMC
April 2021

Global, regional, and national mortality trends of female breast cancer by risk factor, 1990-2017.

BMC Cancer 2021 Apr 24;21(1):459. Epub 2021 Apr 24.

School of Public Health, Guilin Medical University, 1 Zhiyuan Road, Guilin, Guangxi, 541199, P.R. China.

Background: Female breast cancer (FBC) is a malignancy involving multiple risk factors and has imposed heavy disease burden on women. We aim to analyze the secular trends of mortality rate of FBC according to its major risk factors.

Methods: Death data of FBC at the global, regional, and national levels were retrieved from the online database of Global Burden of Disease study 2017. Deaths of FBC attributable to alcohol use, high body-mass index (BMI), high fasting plasma glucose (FPG), low physical activity, and tobacco were collected. Estimated average percentage change (EAPC) was used to quantify the temporal trends of age-standardized mortality rate (ASMR) of FBC in 1990-2017.

Results: Worldwide, the number of deaths from FBC increased from 344.9 thousand in 1990 to 600.7 thousand in 2017. The ASMR of FBC decreased by 0.59% (95% CI, 0.52, 0.66%) per year during the study period. This decrease was largely driven by the reduction in alcohol use- and tobacco-related FBC, of which the ASMR was decreased by 1.73 and 1.77% per year, respectively. In contrast, the ASMR of FBC attributable to high BMI and high FPG was increased by 1.26% (95% CI, 1.22, 1.30%) and 0.26% (95% CI, 0.23, 0.30%) per year between 1990 and 2017, respectively.

Conclusions: The mortality rate of FBC experienced a reduction over the last three decades, which was partly owing to the effective control for alcohol and tobacco use. However, more potent and tailored prevention strategies for obesity and diabetes are urgently warranted.
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http://dx.doi.org/10.1186/s12885-021-08217-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070325PMC
April 2021

Beagle Dogs Have Low Susceptibility to Florida Clade 2 H3N8 Equine Avian Influenza.

Virol Sin 2021 Apr 15. Epub 2021 Apr 15.

Guangdong Provincial Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.

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http://dx.doi.org/10.1007/s12250-021-00366-yDOI Listing
April 2021

Management of renal tumors during pregnancy: case reports.

BMC Nephrol 2021 Apr 9;22(1):127. Epub 2021 Apr 9.

Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Science, Beijing, China.

Background: Renal tumors during pregnancy are rare and the treatment requires evaluation of both the patient and the fetus. No consensus or guidelines has been proposed or verified in this field. We successfully managed three renal tumor cases during pregnancy and reviewed the relative literature.

Case Presentation: In the first renal cell carcinoma case diagnosed in the 21st week of pregnancy, laparoscopic retroperitoneoscopic partial nephrectomy was performed in the 26th week of pregnancy. In the second renal cell carcinoma case diagnosed in the 3rd week of pregnancy, laparoscopic retroperitoneoscopic radical nephrectomy was carried out after the abortion. In the third angiomyolipoma case who was diagnosed before pregnancy but received no treatment, we performed laparoscopic retroperitoneoscopic partial nephrectomy during the 17th week of pregnancy due to the rapid enlargement of the tumor.

Conclusion: Although no consensus or guidelines for the management of renal tumors in pregnant patients has been proposed or verified, the general rules of kidney tumor management in non-pregnant patients and the guidelines for surgery in pregnancy could be referred to. Renal tumors found in pregnant patients require an individualized treatment regimen involving surgical timing, routes, techniques, and excision ranges, which should be decided by both the patients and the surgical teams.
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http://dx.doi.org/10.1186/s12882-021-02318-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035726PMC
April 2021

Comprehensive screen the lead and other toxic metals in total environment from a coal-gas industrial city (NW, China): Based on integrated source-specific risks and site-specific blood lead levels of 0-6 aged children.

Chemosphere 2021 Sep 31;278:130416. Epub 2021 Mar 31.

International Joint Research Centre of Shaanxi Province for Pollutant Exposure and Eco-environmental Health, Xi'an, Shaanxi, 710062, PR China; School of Computer, Mathematical and Natural Sciences, Morgan State University, Baltimore, MD, 21251, USA.

A new integrated source-specific risk model and site-specific blood lead levels (BLLs) of 0-6 children were introduced to comprehensive understand the status of the toxic metals in soil-dust-plant total environment from a Coal-Gas industrial city, NW China. 144 samples were collected and ten toxic metals (As, Ba, Co, Cr, Cu, Mn, Ni, Sr, Pb, and Zn) were screened by XRF and ICP-MS. It was found that the occurrences of toxic metals deferred in the different medium, such as Co, Cu, Pb, and Zn observed the trend of accumulating in soil and plant compared to clustered distributions of Cr, Mn and Ni preferred to accumulate in dust. However, few bioaccumulations observed in Ulmus pumila L. Toxic metals distributions in majority of sites influenced by coal combustion mixed sources and industrial activities posed the high integrated ecological risks and caused significant non-carcinogenic and carcinogenic integrated risks for local 0-6 children identified by new integrated source-specific risk model, especially observed in the priority contaminants Co and Pb. The site-specific BLLs confirmed that younger children fewer than 4 lived in the north region were more vulnerable to priority Pb pollution as their BLLs above 50 μg/L, almost up to 80 μg/L. Although proportions of source-specific risks to toxic metals changed in soil and dust, the critical sources from coal combustions and industrial activities posed the most important contribution to the local risks. Therefore, effective strategies targeting at critical sources on coal industries should be conducted to reduce risks, and mostly emphasize on the north hotspot areas.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130416DOI Listing
September 2021

Association between Sleep Duration, Physical Activity, and Mental Health Disorders: A Secondary Analysis of the National Survey of Children's Health 2017-2018.

Biomed Res Int 2021 15;2021:5585678. Epub 2021 Mar 15.

Hunan Children's Research Institute (HCRI), Hunan Children's Hospital, 86 Ziyuan Road, Changsha, China 410007.

Background: The purpose of this article was to examine the association of sleep duration and physical activity and their interactions on mental health disorders in American children aged 6-17 years.

Methods: Data were analyzed from the combined 2017-2018 National Survey of Children's health. Ultimately, a total of 36370 children aged 6-17 years were selected as the samples. Weighted logistic regression models were used to estimate odds ratios and 95% confidence intervals.

Results: Insufficient sleep duration was associated with an increased risk for current anxiety, depression, and behavior/conduct problems (odds ratio = 1.449, 1.991, 1.375; 95% confidence interval: 1.313-1.702, 1.648-2.406, 1.162-1.627). Insufficient physical activity was associated with an increased risk for current anxiety (odds ratio = 1.448; 95% confidence interval: 1.230-1.706) and depression (odds ratio = 1.743; 95% confidence interval: 1.304-2.329). In addition, additive interactions between sleep duration and physical activity were observed on current anxiety and depression.

Conclusions: Insufficient sleep duration and insufficient physical activity in children were associated with mental health disorders. There is a synergistic interaction effect between insufficient sleep duration and insufficient physical activity on current anxiety and current depression.
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http://dx.doi.org/10.1155/2021/5585678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987431PMC
May 2021

Circadian Rhythm Gene PER3 Negatively Regulates Stemness of Prostate Cancer Stem Cells via WNT/β-Catenin Signaling in Tumor Microenvironment.

Front Cell Dev Biol 2021 18;9:656981. Epub 2021 Mar 18.

Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Prostate cancer (PCa) cells are heterogeneous, containing a variety of cancer cells with phenotypical and functional discrepancies in the tumor microenvironment, where prostate cancer stem cells (PCSCs) play a vital role in PCa development. Our earlier studies have shown that ALDHCD44 (DP) PCa cells and the corresponding ALDHCD44 (DN) PCa cells manifest as PCSCs and non-PCSCs, respectively, but the underlying mechanisms regulating stemness of the PCSCs are not completely understood. To tackle this issue, we have performed RNA-Sequencing and bioinformatic analysis in DP (versus DN) cells in this study. We discovered that, PER3 (period circadian regulator 3), a circadian rhythm gene, is significantly downregulated in DP cells. Overexpression of PER3 in DP cells significantly suppressed their sphere- and colony-forming abilities as well as tumorigenicity in immunodeficient hosts. In contrast, knockdown of PER3 in DN cells dramatically promoted their colony-forming and tumor-initiating capacities. Clinically, PER3 is downregulated in human prostate cancer specimens and PER3 expression levels are highly correlated with the prognosis of the PCa patient. Mechanistically, we observed that low levels of PER3 stimulates the expression of BMAL1, leading to the phosphorylation of β-catenin and the activation of the WNT/β-catenin pathway. Together, our results indicate that PER3 negatively regulates stemness of PCSCs via WNT/β-catenin signaling in the tumor microenvironment, providing a novel strategy to treat PCa patients.
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http://dx.doi.org/10.3389/fcell.2021.656981DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012816PMC
March 2021

Application of low-dose CT to the creation of 3D-printed kidney and perinephric tissue models for laparoscopic nephrectomy.

Cancer Med 2021 05 2;10(9):3077-3084. Epub 2021 Apr 2.

Department of Radiology, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.

Purpose: The aim of this study was to explore the feasibility of 3D printing of kidney and perinephric fat based on low-dose CT technology.

Patients And Methods: A total of 184 patients with stage T1 complex renal tumors who underwent laparoscopic nephrectomy were prospectively enrolled and divided into three groups: group A (conventional dose kidney and perinephric fat 3D printing group, n = 62), group B (low-dose kidney and perinephric fat 3D printing, n = 64), and group C (conventional dose merely kidney 3D printing group, n = 58). The effective dose (ED), signal-to-noise ratio (SNR), and contrast-to-noise ratio (CNR) were determined. The 3D printing quality was evaluated using a 4-point scale, and interobserver agreement was assessed using the intraclass correlation coefficient (ICC).

Results: The ED of group B was lower than that of group A, with a decrease of 55.1%. The subjective scores of 3D printing quality in all groups were 3 or 4 points. The interobserver agreement among the three observers in 3D printing quality was good (ICC = 0.84-0.92). The perioperative indexes showed that operation time (OT), warm ischemia time (WIT), estimated blood loss (EBL), and laparoscopic partial nephrectomy (LPN) conversion to laparoscopic radical nephrectomy (LRN) in groups A or B were significantly less than those in group C. LPN was more frequent in group A and group B than in group C (all p < 0.017). There were no significant differences in perioperative indexes between group A and group B (all p > 0.017).

Conclusion: Low-dose CT technology can be effectively applied to 3D printing of kidney and perinephric fat and reduce the patient's radiation dose without compromising 3D printing quality. 3D printing of kidney and perinephric fat can significantly increase the success rate of LPN and decrease OT, WIT, and EBL.
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http://dx.doi.org/10.1002/cam4.3851DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085913PMC
May 2021

H3.1K27me1 maintains transcriptional silencing and genome stability by preventing GCN5-mediated histone acetylation.

Plant Cell 2021 May;33(4):961-979

Yale University, Department of Molecular, Cellular and Developmental Biology, Faculty of Arts and Sciences, 260 Whitney Avenue, New Haven, CN 06511.

Epigenetic mechanisms play diverse roles in the regulation of genome stability in eukaryotes. In Arabidopsis thaliana, genome stability is maintained during DNA replication by the H3.1K27 methyltransferases ARABIDOPSIS TRITHORAX-RELATED PROTEIN 5 (ATXR5) and ATXR6, which catalyze the deposition of K27me1 on replication-dependent H3.1 variants. The loss of H3.1K27me1 in atxr5 atxr6 double mutants leads to heterochromatin defects, including transcriptional de-repression and genomic instability, but the molecular mechanisms involved remain largely unknown. In this study, we identified the transcriptional co-activator and conserved histone acetyltransferase GCN5 as a mediator of transcriptional de-repression and genomic instability in the absence of H3.1K27me1. GCN5 is part of a SAGA-like complex in plants that requires the GCN5-interacting protein ADA2b and the chromatin remodeler CHR6 to mediate the heterochromatic defects in atxr5 atxr6 mutants. Our results also indicate that Arabidopsis GCN5 acetylates multiple lysine residues on H3.1 variants, but H3.1K27 and H3.1K36 play essential functions in inducing genomic instability in the absence of H3.1K27me1. Finally, we show that H3.1K36 acetylation by GCN5 is negatively regulated by H3.1K27me1 in vitro. Overall, this work reveals a key molecular role for H3.1K27me1 in maintaining transcriptional silencing and genome stability in heterochromatin by restricting GCN5-mediated histone acetylation in plants.
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http://dx.doi.org/10.1093/plcell/koaa027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226292PMC
May 2021

Can spot urine measurement be a substitute for 24-hour urine measurement to estimate sodium intake in adolescents?

Pediatr Investig 2021 Mar 22;5(1):12-20. Epub 2021 Mar 22.

Department of Epidemiology Capital Institute of Pediatrics Beijing China.

Importance: Several methods have been established in recent decades that allow use of spot urine to estimate dietary sodium intake. However, their accuracies have been controversial in children.

Objective: To validate the performance of three commonly used methods-the Kawasaki, Tanaka, and International Cooperative Study on Salt, Other Factors, and Blood Pressure (INTERSALT) methods. Additionally, this study explored the accuracies of the Tanaka and INTERSALT methods by using spot urine samples taken at four separate times.

Method: Forty-one adolescents aged 14 to 16 years completed two non-consecutive 24-hour urine collections and their mean values were used as reference data. The second-morning urine was used for assessment with the Kawasaki method; a casual spot urine and spot urine samples taken at four separate times (morning, afternoon, evening, and overnight) were used for assessment with the Tanaka and INTERSALT methods.

Results: The mean differences were 1801 mg, 542 mg, 47 mg, and -31 mg for the Kawasaki, Tanaka, INTERSALT1 (with potassium), and INTERSALT2 (without potassium) methods with their required spot urine, respectively. The proportions of relative difference levels within ± 10% were 4.9% for the Kawasaki method, 19.5% for the Tanaka method, 36.6% for the INTERSALT1 method, and 36.6% for the INTERSALT2 method.

Interpretation: The INTERSALT method seemed to provide minimally biased estimations of mean population sodium intake with casual spot urine. However, there is a need to be cautious regarding inconsistencies in estimation among different levels of sodium intake. The methods assessed in this study were unable to accurately estimate sodium intake at the individual level.
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http://dx.doi.org/10.1002/ped4.12243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984014PMC
March 2021

RNA-seq analysis of antibacterial mechanism of essential oil against .

PeerJ 2021 17;9:e11081. Epub 2021 Mar 17.

Human Aging Research Institute and School of Life Science and Jiangxi Key Laboratory of Human Aging, Nanchang University, Nanchang, Jiangxi, China.

Background: Transcriptome analysis plays a central role in elucidating the complexity of gene expression regulation in . In recent years, the overuse of antibiotics has led to an increase in antimicrobial resistance, which greatly reduces the efficacy of antibacterial drugs and affects people's health. Therefore, several researchers are focused on finding other materials, which could replace or supplement antibiotic treatment.

Methods: was treated with water, acetone and essential oils, respectively. The antibacterial activity was assessed using the minimum inhibitory concentration (MIC), the minimum bactericidal concentration (MBC), the dry weight and the wet weight of the cells. To explore the antibacterial mechanism of the oil, the RNA-Seq analysis was adopted under three different treatments. Finally, the expression of related genes was verified by Quantitative PCR.

Results: In this study, we showed that the essential oil exerted a strong antibacterial effect. Our results showed that the inhibitory efficiency increased with increasing of the concentration of essential oil. RNA-seq analysis indicated that the essential oil inhibited the growth of by inhibiting the metabolism, chemotaxis, and adhesion, meanwhile, life activities were maintained by enhancing resistance reactions. These results are contributed to uncover the antimicrobial mechanisms of essential oils against , and the essential oil could be applied as an antibacterial agent to replace or ally with antibiotic.
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http://dx.doi.org/10.7717/peerj.11081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7980702PMC
March 2021