Publications by authors named "Jiayi Chen"

224 Publications

System dynamics analysis on the effectiveness of vaccination and social mobilization policies for COVID-19 in the United States.

PLoS One 2022 12;17(8):e0268443. Epub 2022 Aug 12.

Department of Industrial Management, National Taiwan University of Science and Technology, Taipei, Taiwan, ROC.

The COVID-19 pandemic has presented significant public health and economic challenges worldwide. Various health and non-pharmaceutical policies have been adopted by different countries to control the spread of the virus. To shed light on the impact of vaccination and social mobilization policies during this wide-ranging crisis, this paper applies a system dynamics analysis on the effectiveness of these two types of policies on pandemic containment and the economy in the United States. Based on the simulation of different policy scenarios, the findings are expected to help decisions and mitigation efforts throughout this pandemic and beyond.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0268443PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374237PMC
August 2022

Rainbow latex microspheres lateral flow immunoassay with smartphone-based device for simultaneous detection of three mycotoxins in cereals.

Anal Chim Acta 2022 Aug 30;1221:340138. Epub 2022 Jun 30.

Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

The coexistent pollution of multiple mycotoxins displays a synergistic toxicity effect that significantly threatens human health. Therefore, it is essential to establish a rapid detection method for multi-mycotoxins in food. In this study, red, green, and blue latex microspheres (LMs) were applied as the aflatoxin B (AFB), T-2 toxins (T-2), and zearalenone (ZEN) antibodies labeled tracer, respectively. Based on the principle of spatial resolution, a rainbow "traffic light" pattern latex microspheres lateral flow immunoassay (LMs-LFIA) integrated with a portable and user-friendly smartphone-based device was first developed to detect three kinds of mycotoxins simultaneously. The cut-off values of the method for AFB/T-2/ZEN in cereals were 1/15/40 μg kg, the limits of detection were 0.04/0.40/1.21 μg kg, respectively. The recoveries ranged from 82.1% to 107.5%, with the coefficient of variation from 3.0% to 8.1%. A parallel analysis in 26 naturally contaminated cereal samples was confirmed by commercial ELISA kits; the results showed a good correlation (R>0.99), indicating the practical reliability of the rainbow LMs-LFIA. This method provided a visually enjoyable, portable, and sensitive detection mode for multi-target detection of mycotoxins or other small molecule hazard factors in food.
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http://dx.doi.org/10.1016/j.aca.2022.340138DOI Listing
August 2022

A proposed disease classification system for duck viral hepatitis.

Poult Sci 2022 Jul 1;101(9):102042. Epub 2022 Jul 1.

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China. Electronic address:

The nomenclature of duck viral hepatitis (DVH) was historically not a problem. However, 14 hepatotropic viruses among 10 different genera are associated with the same disease name, DVH. Therefore, the disease name increasingly lacks clarity and may no longer fit the scientific description of the disease. Because one disease should not be attributed to 10 genera of viruses, this almost certainly causes misunderstanding regarding the disease-virus relationship. Herein, we revisited the problem and proposed an update to DVH disease classification. This classification is based on the nomenclature of human viral hepatitis and the key principle of Koch's postulates ("one microbe and one disease"). In total, 10 types of disease names have been proposed. These names were literately matched with hepatitis-related viruses. We envision that this intuitive nomenclature system will facilitate scientific communication and consistent interpretation in this field, especially in the Asian veterinary community, where these diseases are most commonly reported.
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http://dx.doi.org/10.1016/j.psj.2022.102042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334327PMC
July 2022

The Effectiveness of Intermittent Theta Burst Stimulation for Stroke Patients With Upper Limb Impairments: A Systematic Review and Meta-Analysis.

Front Neurol 2022 6;13:896651. Epub 2022 Jul 6.

Department of Rehabilitation Medicine, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Background: Upper limb impairments are one of the most common health problems of stroke, affecting both motor function and independence in daily life. It has been demonstrated that intermittent theta burst stimulation (iTBS) increases brain excitability and improves upper limb function. Our study sought to determine the role of iTBS in stroke recovery.

Objective: The purpose of this study was to determine the efficacy of iTBS in individuals with upper limb impairments following stroke.

Methods: The databases used included Cumulative Index to PubMed, EMBASE, ESCBOhost, The Cochrane Library, Chinese Biomedical Database, Web of Science, China Biology Medicine (CBM), China National Knowledge Infrastructure (CNKI), Technology Periodical Database (VIP), and WanFang Database. Studies published before November 2021 were included. Each participant received an iTBS-based intervention aimed at improving activity levels or impairment, which was compared to usual care, a sham intervention, or another intervention. The primary outcome measure was a change in upper limb function assessment. Secondary outcomes included impairment, participation, and quality of life measures.

Result: A total of 18 studies ( = 401 participants) that met the inclusion criteria were included in this study. There was a slight change in the upper limb function of the iTBS group compared with the control group, as measured by the Fugl-Meyer Assessment-Upper Extremity (FMA-UE) score (mean difference 2.70, 95% CI -0.02 to 5.42, = 0.05). Significant improvement in resting motor threshold (RMT) and motor-evoked potential (MEP) was also observed in the meta-analysis of iTBS (MD 3.46, 95% CI 2.63 to 4.28, < 0.00001); (MD 1.34, 95% CI 1.17 to 1.51, < 0.00001). In addition, we got similar results when the studies were using the Modified Barthel Index (MBI) assessment (mean difference of 7.34, 95% CI 0.47 to 14.21, = 0.04).

Conclusion: Our study established the efficacy of iTBS in improving motor cortical plasticity, motor function, and daily functioning in stroke patients. However, the review requires evidence from additional randomized controlled trials and high-quality research.

Systematic Review Registration: https://www.crd.york.ac.uk/PROSPERO/.
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http://dx.doi.org/10.3389/fneur.2022.896651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298981PMC
July 2022

Chitosan Oligosaccharide Inhibits the Synthesis of Milk Fat in Bovine Mammary Epithelial Cells through AMPK-Mediated Downstream Signaling Pathway.

Animals (Basel) 2022 Jun 30;12(13). Epub 2022 Jun 30.

College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.

Chitosan oligosaccharide (COS) is a variety of oligosaccharides, and it is also the only abundant basic amino oligosaccharide in natural polysaccharides. Chitosan oligosaccharide is a low molecular weight product of chitosan after enzymatic degradation. It has many biological effects, such as lipid-lowering, antioxidant and immune regulation. Previous studies have shown that chitosan oligosaccharide has a certain effect on fat synthesis, but the effect of chitosan oligosaccharide on milk fat synthesis of bovine mammary epithelial cells (BMECs) has not been studied. Therefore, this study aimed to investigate chitosan oligosaccharide's effect on milk fat synthesis in bovine mammary epithelial cells and explore the underlying mechanism. We treated bovine mammary epithelial cells with different concentrations of chitosan oligosaccharide (0, 100, 150, 200, 400 and 800 μg/mL) for 24 h, 36 h and 48 h respectively. To assess the effect of chitosan oligosaccharide on bovine mammary epithelial cells and determine the concentration and time for chitosan oligosaccharide treatment on cells, several in vitro cellular experiments, including on cell viability, cycle and proliferation were carried out. The results highlighted that chitosan oligosaccharide (100, 150 μg/mL) significantly promoted cell viability, cycle and proliferation, increased intracellular cholesterol content, and reduced intracellular triglyceride and non-esterified fatty acids content. Under the stimulation of chitosan oligosaccharide, the expression of genes downstream of Phosphorylated AMP-activated protein kinase () and AMP-activated protein kinase () signaling pathway changed, increasing the expression of peroxisome proliferator-activated receptor alpha () and hormone-sensitive lipase (), but the expression of sterol regulatory element-binding protein 1c () and its downstream target gene stearoyl-CoA desaturase () decreased. In conclusion, these results suggest that chitosan oligosaccharide may inhibit milk fat synthesis in bovine mammary epithelial cells by activating the AMP-activated protein kinase signaling pathway, promoting the oxidative decomposition of fatty acids and inhibiting fatty acid synthesis.
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http://dx.doi.org/10.3390/ani12131692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265072PMC
June 2022

Arsenic-induced up-regulation of P transporters PvPht1;3-1;4 enhances both As and P uptake in As-hyperaccumulator Pteris vittata.

J Hazard Mater 2022 Jun 23;438:129430. Epub 2022 Jun 23.

Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address:

Plants often up-regulate gene expression of P transporters under P deficiency, but down-regulate them under arsenic stress. Different from other P transporters, PvPht1;3 and PvPht1;4 expressions in As-hyperaccumulator Pteris vittata are up-regulated under P deficiency and As stress, showing strong transport capacity for both As and P. This study examined the mechanisms behind As-induced up-regulation of P transporters in P. vittata after exposing to 10-50 µM arsenate (AsV) for 14 d under hydroponics, with non-hyperaccumulator P. ensiformis as a control. Under As stress, P. vittata was more efficient in taking up both As and P than P. ensiformis, showing 48-84% more P content in the fronds and roots, leading to 18-79% greater biomass. Though As enhanced the P uptake by P. vittata, the inorganic P was reduced by 25-64% from 140-347 to 65-126 mg kg. It is likely that, under As stress, more P was utilized by P. vittata to counter As toxicity, causing reduction in inorganic P. This was supported by As-induced conversion of inorganic P to phytate in P. vittata, with phytate-P being increased by 26-75% from 239-713 to 418-1221 mg kg, maintaining internal low P levels. Under As-induced low inorganic-P conditions, the expressions of P transporters PvPht1;3 and PvPht1;4 were up-regulated by 1.4- and 2.7-fold in the roots, helping greater As and P uptake by P. vittata. Clearly, As-induced overexpression of P transporters in P. vittata roots plays a critical role in taking up both As and P, thereby increasing its efficiency in As-hyperaccumulation from contaminated media.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129430DOI Listing
June 2022

Threonine supplementation prevents the development of fat deposition in mice fed a high-fat diet.

Food Funct 2022 Jul 18;13(14):7772-7780. Epub 2022 Jul 18.

Institute of Animal Nutrition, Northeast Agricultural University, Harbin, 150030, China.

Obesity is the main factor involved in the onset of many diseases. Threonine supplementation has been demonstrated to reduce fat mass and serum triglycerides in already obese mice. However, it is unclear whether threonine could inhibit the development of obesity in mice without previous high-fat diet induction. In the present study, mice were fed a chow diet (CD) or a high-fat diet (HFD), supplemented or not with threonine (3.0% in drinking water) for 15 weeks. Results showed that mice subjected to chronic threonine supplementation showed decreased body weight, epididymal white adipose tissue weight, serum low-density lipoprotein cholesterol, and total cholesterol in comparison with HFD-fed mice. In the epididymal adipose tissue, gene expressions of sterol regulatory element-binding protein 1c and fatty acid synthase were up-regulated, while hormone sensitive lipase, adiponectin and fibroblast growth factor 21 were down-regulated. In the liver tissue, gene expressions of sirtuin1, adenosine monophosphate-activated protein kinase and peroxisome proliferator activated receptor γ co-activator 1α were up-regulated by threonine supplementation in HFD-fed mice. These results suggest that long-term threonine supplementation inhibited fat mass and improved lipid metabolism, making it a potential agent to prevent the development of diet-induced obesity.
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http://dx.doi.org/10.1039/d2fo01201dDOI Listing
July 2022

Biomass waste as a clean reductant for iron recovery of iron tailings by magnetization roasting.

J Environ Manage 2022 Sep 4;317:115435. Epub 2022 Jun 4.

Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou, 510006, China.

The magnetization roasting with coal as primary reductants adds cost and causes environmental pollution. Therefore, it is of great importance to investigate the biomass application as a reductant for magnetization roasting to recover iron from low-utilization iron tailings for emission mitigation and green utilization. This study systematically investigated the impact of biomass (pyrolysis gas from agricultural and forestry waste) as a reductant on the conversion of iron tailings to magnetite in magnetization roasting. Additionally, the thermal decomposition of biomass, phase transformation and microstructure evolution of iron tailings were analyzed by TG, XRD, BET, and other methods to elucidate the conversion mechanism for facilitating magnetized hematite in iron tailings with biomass-derived gas. The results showed that woody biomass was a more appropriate reductant for magnetization roasting; 650 °C was the optimal temperature for the complete transformation of hematite to magnetite by reduction roasting with biomass waste. Through magnetic separation, the concentrate with an iron grade of 62.04% and iron recovery of 95.29% was obtained, and the saturation magnetization was enhanced from 0.60 emu/g to 58.03 emu/g of iron tailings. During the magnetization roasting, CO and H generated from biomass reduced the hematite in tailings particles from interior to exterior, forming a loose structure with rich microfissures, facilitating the subsequent separation operations. This study offers a novel reference for applying biomass to exploit hematite minerals and shows the potential of biomass for energy savings and emission reduction in the utilization of iron tailing resources.
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http://dx.doi.org/10.1016/j.jenvman.2022.115435DOI Listing
September 2022

Effects of Dietary Fucoidan Supplementation on Serum Biochemical Parameters, Small Intestinal Barrier Function, and Cecal Microbiota of Weaned Goat Kids.

Animals (Basel) 2022 Jun 20;12(12). Epub 2022 Jun 20.

College of Coastal Agriculture Science, Guangdong Ocean University, Zhanjiang 524088, China.

The purpose of this study was to evaluate the effects of fucoidan supplementation on serum biochemical parameters, small intestinal barrier function, and cecal microbiota of weaned goat kids. A total of 60 2-month-old weaned castrated male goat kids (Chuanzhong black goat) were used in this 30-day experiment. The goat kids were randomly divided into four groups: a control group (CON) fed the basal diet, and three other groups supplemented with 0.1%, 0.3%, and 0.5% fucoidan in the basal diet (denoted as F1, F2, and F3 groups, respectively). The results indicated that dietary fucoidan supplementation decreased ( < 0.05) the activity of lactate dehydrogenase (LDH) and the content of glucose (GLU) as measured on day 15. As measured on day 30, dietary fucoidan increased ( < 0.05) the content of total protein (TP) and decreased the activity of aspartate aminotransferase (AST), and supplementation with 0.3% and 0.5% fucoidan decreased ( < 0.05) the activity of LDH. Dietary fucoidan decreased ( < 0.05) the content of D-lactic acid (D-LA) and the activity of diamine oxidase (DAO). Dietary fucoidan increased ( < 0.05) the activity of catalase (CAT) in the duodenum. Dietary 0.3% and 0.5% fucoidan enhanced ( < 0.05) the activity of glutathione peroxidase (GSH-Px) in the ileum, the activity of total superoxide dismutase (T-SOD) in the jejunum and ileum, and the activity of CAT in the ileum. Dietary 0.3% and 0.5% fucoidan reduced the contents of malondialdehyde (MDA) in the duodenum, jejunum, and ileum and the content of hydrogen peroxide (HO) in the duodenum. Dietary fucoidan increased ( < 0.05) the content of secretory immunoglobulin A (sIgA) in the duodenum. Supplementation of 0.3% and 0.5% fucoidan upregulated ( < 0.05) the gene expression of ZO-1 and claudin-1 in the duodenum, jejunum, and ileum, and dietary supplementation of 0.3% and 0.5% fucoidan upregulated ( < 0.05) the gene expression of occludin in the jejunum and ileum. The 16S rRNA high-throughput sequencing results showed that at the phylum level, dietary fucoidan increased ( < 0.05) the abundance of while decreasing ( < 0.05) the abundance of . At the genus level, dietary 0.3% and 0.5% fucoidan increased ( < 0.05) the abundances of , , and In conclusion, dietary fucoidan supplementation had positive effects on intestinal permeability, antioxidant capacity, immunity function, tight junctions, and the cecal microflora balance in weaned goat kids.
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http://dx.doi.org/10.3390/ani12121591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219480PMC
June 2022

Mapping of PET/CT-based regional nodes distribution of recurrent/advanced breast cancer and comparison with current delineation atlas.

Br J Radiol 2022 Aug 2:20220382. Epub 2022 Aug 2.

Department of Radiation Oncology, Ruijin Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.

Objective: To localize the distribution of regional nodes in recurrent/advanced breast cancer patients based on 18-fludeoxyglucose (FDG) positron emission tomography/CT (PET/CT) images and validate the coverage of clinical target volumes (CTVs) for regional nodes with current contouring guidelines.

Methods: We enrolled 154 recurrent/advanced breast cancer patients with FDG-avid regional nodes who underwent PET/CT between January 2018 and June 2020. Involvement of lymph node regions including axillary lymph node level I-III (ALN-I, ALN-II, ALN-III), Rotter's nodes (RN), medial supraclavicular (SC-M), lateral supraclavicular (SC-L) and internal mammary nodes (IMN) was recorded respectively. Coverage of the CTVs in different atlases and the locations of out-of-field were evaluated.

Results: A total of 348 lymph node regions containing disease were identified, including ALN-I 109, ALN-II 46, ALN-III 36, RN 17, SC-M 68, SC-L 36 and IMN 36. Recurrent ALNs mainly located cranially and ventrally to the axillary vein (AV). Ipsilateral cervical nodes were simultaneously affected in 33/76 SC positive patients. RADCOMP (306/348) and RUIJIN (291/348) guidelines had higher coverage compared with RTOG (205/348) and ESTRO (202/348) guidelines ( < 0.001, respectively). In primary non-metastastic and recurrent patients, major missings located in SC-L (7/7, 17/17) and IMN (7/10, 15/19) for RTOG guideline while SC-L (7/7, 17/17) for ESTRO guideline ( < 0.001, respectively). Among recurrent patients, SC-M (22/31) was another major missing area for ESTRO guideline ( < 0.001).

Conclusion: The current guidelines effectively cover most regional nodes in postoperative breast cancer patients. SC-L and IMN were the major missing regions. Recurrent ALNs were most often seen in cranial and ventral to the AV. The CTV of patients with clinically positive SC was recommended to extend up to the hyoid level. The CTVs should be adjusted based on risks of recurrence individually.

Advances In Knowledge: The difference of regional nodes delineation between current guidelines mainly located in SC and IMN regions. High axilla including subclavicular nodes and the RN above AV for recurrent patients and the region between cricoid and hyoid for positive SC patients should be meticulously contoured.
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http://dx.doi.org/10.1259/bjr.20220382DOI Listing
August 2022

Morphological and Spatial Heterogeneity of Microbial Communities in Pilot-Scale Autotrophic Integrated Fixed-Film Activated Sludge System Treating Coal to Ethylene Glycol Wastewater.

Front Microbiol 2022 2;13:927650. Epub 2022 Jun 2.

Beijing International Scientific and Technological Cooperation Base of Water Pollution Control Techniques for Antibiotics and Resistance Genes, Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Environment, Beijing Jiaotong University, Beijing, China.

The understanding of microbial compositions in different dimensions is essential to achieve the successful design and operation of the partial nitritation/anammox (PN/A) process. This study investigated the microbial communities of different sludge morphologies and spatial distribution in the one-stage PN/A process of treating real coal to ethylene glycol (CtEG) wastewater at a pilot-scale integrated fixed-film activated sludge (IFAS) reactor. The results showed that ammonia-oxidizing bacteria (AOB) was mainly distributed in flocs (13.56 ± 3.16%), whereas anammox bacteria (AnAOB) was dominated in the biofilms (17.88 ± 8.05%). Furthermore, the dominant AnAOB genus in biofilms among the first three chambers was (6.46 ± 2.14% to 11.82 ± 6.33%), whereas it was unexpectedly transformed to (9.47 ± 1.70%) and (8.56 ± 4.69%) in the last chamber. This demonstrated that the niche differentiation resulting from morphological (dissolved oxygen) and spatial heterogeneity (gradient distribution of nutrients and toxins) was the main reason for dominant bacterial distribution. Overall, this study presents more comprehensive information on the heterogeneous distribution and transformation of communities in PN/A processes, providing a theoretical basis for targeted culture and selection of microbial communities in practical engineering.
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http://dx.doi.org/10.3389/fmicb.2022.927650DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201488PMC
June 2022

Caffeine excites medial parabrachial nucleus neurons of mice by blocking adenosine A1 receptor.

Brain Res 2022 09 13;1790:147984. Epub 2022 Jun 13.

Department of Pediatrics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, 109 Xueyuan Western Road, Wenzhou, Zhejiang 325027, PR China; The Second School of Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, PR China. Electronic address:

Caffeine has been used as a first-line drug for treatment of apnea neonatorum for decades due to its high safety and effectiveness. Studies report that caffeine mainly acts as a blocker of Adenosine Receptors (ARs). However, the mechanism of caffeine in reducing apnea neonatorum in the central nervous system has not been fully explored. Medial parabrachial nucleus (MPB) is part of the respiratory center of the pons that may be related to the activity of caffeine. Previous studies have not explored the effect and mechanism of caffeine on MPB neurons. To elucidate this, the current study used antagonists of A1 and A2a receptors to mimic the effect of caffeine in MPB of mice in vitro using the patch-clamp technique. The firing rates and spontaneous post-synaptic currents were recorded. The findings of the study showed that caffeine excited MPB neurons. Notably, the adenosine A1R antagonist 8-cyclopentyl-1,3-dimethyl-xanthine (CPT) but not the adenosine A2aR antagonist Istradefylline (KW6002) mimicked the exciting effect of caffeine, implying that caffeine excited MPB neurons in mice by blocking A1Rs. Further, the results indicated that caffeine could increase efficiency of synaptic transmission to excite MPB neurons. These findings suggest that A1Rs in MPB may be potential targets for caffeine in reducing apnea neonatorum.
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http://dx.doi.org/10.1016/j.brainres.2022.147984DOI Listing
September 2022

Severe Acute Respiratory Syndrome Coronavirus 2 ORF8 Protein Inhibits Type I Interferon Production by Targeting HSP90B1 Signaling.

Front Cell Infect Microbiol 2022 23;12:899546. Epub 2022 May 23.

BSL-3 Laboratory (Guangdong), Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a global pandemic that has currently infected over 430 million individuals worldwide. With the variant strains of SARS-CoV-2 emerging, a region of high mutation rates in ORF8 was identified during the early pandemic, which resulted in a mutation from leucine (L) to serine (S) at amino acid 84. A typical feature of ORF8 is the immune evasion by suppressing interferon response; however, the mechanisms by which the two variants of ORF8 antagonize the type I interferon (IFN-I) pathway have not yet been clearly investigated. Here, we reported that SARS-CoV-2 ORF8L and ORF8S with no difference inhibit the production of IFN-β, MDA5, RIG-I, ISG15, ISG56, IRF3, and other IFN-related genes induced by poly(I:C). In addition, both ORF8L and ORF8S proteins were found to suppress the nuclear translocation of IRF3. Mechanistically, the SARS-CoV-2 ORF8 protein interacts with HSP90B1, which was later investigated to induce the production of IFN-β and IRF3. Taken together, these results indicate that SARS-CoV-2 ORF8 antagonizes the RIG-I/MDA-5 signaling pathway by targeting HSP90B1, which subsequently exhibits an inhibitory effect on the production of IFN-I. These functions appeared not to be influenced by the genotypes of ORF8L and ORF8S. Our study provides an explanation for the antiviral immune suppression of SARS-CoV-2 and suggests implications for the pathogenic mechanism and treatment of COVID-19.
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http://dx.doi.org/10.3389/fcimb.2022.899546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168264PMC
May 2022

Predictive performance of different NTCP techniques for radiation-induced esophagitis in NSCLC patients receiving proton radiotherapy.

Sci Rep 2022 06 2;12(1):9178. Epub 2022 Jun 2.

Department of Radiation Physics, Unit 1150, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX, 77030, USA.

This study aimed to compare the predictive performance of different modeling methods in developing normal tissue complication probability (NTCP) models for predicting radiation-induced esophagitis (RE) in non-small cell lung cancer (NSCLC) patients receiving proton radiotherapy. The dataset was composed of 328 NSCLC patients receiving passive-scattering proton therapy and 41.6% of the patients experienced ≥ grade 2 RE. Five modeling methods were used to build NTCP models: standard Lyman-Kutcher-Burman (sLKB), generalized LKB (gLKB), multivariable logistic regression using two variable selection procedures-stepwise forward selection (Stepwise-MLR), and least absolute shrinkage and selection operator (LASSO-MLR), and support vector machines (SVM). Predictive performance was internally validated by a bootstrap approach for each modeling method. The overall performance, discriminative ability, and calibration were assessed using the Negelkerke R, area under the receiver operator curve (AUC), and Hosmer-Lemeshow test, respectively. The LASSO-MLR model showed the best discriminative ability with an AUC value of 0.799 (95% confidence interval (CI): 0.763-0.854), and the best overall performance with a Negelkerke R value of 0.332 (95% CI: 0.266-0.486). Both of the optimism-corrected Negelkerke R values of the SVM and sLKB models were 0.301. The optimism-corrected AUC of the gLKB model (0.796) was higher than that of the SVM model (0.784). The sLKB model had the smallest optimism in the model variation and discriminative ability. In the context of classification and probability estimation for predicting the NTCP for radiation-induced esophagitis, the MLR model developed with LASSO provided the best predictive results. The simplest LKB modeling had similar or even better predictive performance than the most complex SVM modeling, and it was least likely to overfit the training data. The advanced machine learning approach might have limited applicability in clinical settings with a relatively small amount of data.
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http://dx.doi.org/10.1038/s41598-022-12898-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163134PMC
June 2022

High-voltage and short-time dielectric barrier discharge plasma treatment affects structural and digestive properties of Tartary buckwheat starch.

Int J Biol Macromol 2022 Jul 30;213:268-278. Epub 2022 May 30.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, PR China. Electronic address:

To systematically explore the effects of high-voltage and short-time (HV-ST) dielectric barrier discharge (DBD) plasma treatment on Tartary buckwheat starch (TBS), TBS was treated at 15 and 20 kV for 20 and 40 s. Compared to native starch, more corrosions and holes were observed on the surfaces of plasma-modified TBS observed by SEM. Increased crystallinity and short-range structure order in plasma-modified TBS were determined using XRD and FT-IR respectively, while the average chain length and amylose content decreased, with lowest values (13.5 and 6.9%) in sample 20-40. Meanwhile, the solubility and paste clarity of plasma-modified TBS increased, whereas the viscosities decreased, enhancing in vitro digestibility with highest value (79.5%) in sample 20-40. These changes of TBS properties positively correlated with the treatment voltage and time length. Therefore, HV-ST DBD plasma treatment served as an effective tool for altering the properties of TBS. It is favorable for the applications of starch ingredients with low viscosity and high paste clarity, as well as accelerating starch hydrolyzation processes, such as brewing and food fermentation.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.05.171DOI Listing
July 2022

The joint effects of physical activity and air pollution on type 2 diabetes in older adults.

BMC Geriatr 2022 06 1;22(1):472. Epub 2022 Jun 1.

West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu Sichuan, China.

Background: Older adults with type 2 diabetes are at higher risk of developing common geriatric syndromes and have a lower quality of life. To prevent type 2 diabetes in older adults, it's unclear whether the health benefits of physical activity (PA) will be influenced by the harms caused by increased exposure to air pollution during PA, especially in developing countries with severe air pollution problem. We aimed to investigate the joint effects of PA and long-term exposure to air pollution on the type 2 diabetes in older adults from China.

Methods: This cross-sectional study was based on the China Multi-Ethnic cohort (CMEC) study. The metabolic equivalent of PA was calculated according to the PA scale during the CMEC baseline survey. High resolution air pollution datasets (PM, PM and PM) were collected from open products. The joint effects were assessed by the marginal structural mean model with generalized propensity score.

Results: A total of 36,562 participants aged 50 to 79 years were included in the study. The prevalence of type 2 diabetes was 10.88%. The mean (SD) level of PA was 24.93 (18.60) MET-h/d, and the mean (SD) level of PM, PM, and PM were 70.00 (23.32) µg/m, 40.45 (15.66) µg/m and 27.62 (6.51) µg/m, respectively. With PM < 92 µg/m, PM < 61 µg/m, and PM < 36 µg/m, the benefit effects of PA on type 2 diabetes was significantly greater than the harms due to PMs when PA levels were roughly below 80 MET-h/d. With PM ≥ 92 µg/m, PM ≥ 61 µg/m, and PM ≥ 36 µg/m, the odds ratio (OR) first decreased and then rose rapidly with confidence intervals progressively greater than 1 and break-even points close to or even below 40 MET-h/d.

Conclusions: Our findings implied that for the prevention of type 2 diabetes in older adults, the PA health benefits outweighed the harms of air pollution except in extreme air pollution situations, and suggested that when the air quality of residence is severe, the PA levels should ideally not exceed 40 MET-h/d.
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http://dx.doi.org/10.1186/s12877-022-03139-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9158242PMC
June 2022

Fusion of multimodality image and point cloud for spatial surface registration for knee arthroplasty.

Int J Med Robot 2022 May 30:e2426. Epub 2022 May 30.

Digital Medical Research Center, School of Basic Medical Sciences, Fudan University, Shanghai, China.

Background: Image-guided computer-aided navigation system is an indispensable part of computer assisted orthopaedic surgery. However, the location and number of fiducial markers, the time required to localise fiducial markers in existing systems affect their effectiveness.

Method: The study proposed that spatial surface registration between the point cloud on the surface of the fusion model based on preoperative knee MRI and CT images and the point cloud on the cartilage surface captured by intraoperative laser scanner could solve the above limitations.

Results: The experimental results show that the registration error of the method is less than 2 mm, but the total time from scanning the point cloud on patient's cartilage surface to registering it with the point cloud in preoperative image space is less than 2 min.

Conclusion: The method achieves the registration accuracy similar to existing methods without selecting anatomical corresponding points, which is of great help to the clinic.
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http://dx.doi.org/10.1002/rcs.2426DOI Listing
May 2022

β-amyloid protein induces mitophagy-dependent ferroptosis through the CD36/PINK/PARKIN pathway leading to blood-brain barrier destruction in Alzheimer's disease.

Cell Biosci 2022 May 26;12(1):69. Epub 2022 May 26.

Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Introduction: Blood-brain barrier (BBB) dysfunction may occur at the onset of Alzheimer's disease (AD). Pericytes are a vital part of the neurovascular unit and the BBB, acting as gatekeepers of the BBB. Amyloid β (Aβ) deposition and neurofibrillary tangles in the brain are the central pathological features of AD. CD36 promotes vascular amyloid deposition and leads to vascular brain damage, neurovascular dysfunction, and cognitive deficits. However, the molecular mechanism by which pericytes of the BBB are disrupted remains unclear.

Objectives: To investigate the effect of low-dose Aβ1-40 administration on pericyte outcome and the molecular mechanism of BBB injury.

Methods: We selected 6-month-old and 9-month-old APP/PS1 mice and wild-type (WT) mice of the same strain, age, and sex as controls. We assessed the BBB using PET/CT. Brain pericytes were extracted and cocultured with endothelial cells (bEnd.3) to generate an in vitro BBB model to observe the effect of Aβ1-40 on the BBB. Furthermore, we explored the intracellular degradation and related molecular mechanisms of Aβ1-40 in cells.

Results: BBB permeability and the number of pericytes decreased in APP/PS1 mice. Aβ1-40 increased BBB permeability in an in vivo model and downregulated the expression of CD36, which reversed the Aβ-induced changes in BBB permeability. Aβ1-40 was uptaked in pericytes with high CD36 expression. We observed that this molecule inhibited pericyte proliferation, caused mitochondrial damage, and increased mitophagy. Finally, we confirmed that Aβ1-40 induced pericyte mitophagy-dependent ferroptosis through the CD36/PINK1/Parkin pathway.

Conclusion: PDGFRβ (a marker of pericytes), CD36, and Aβ colocalized in vitro and in vivo, and Aβ1-40 caused BBB disruption by upregulating CD36 expression in pericytes. The mechanism by which Aβ1-40 destroys the BBB involves the induction of pericyte mitophagy-dependent ferroptosis through the CD36/PINK1/Parkin pathway.
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http://dx.doi.org/10.1186/s13578-022-00807-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134700PMC
May 2022

Alantolactone inhibits melanoma cell culture viability and migration and promotes apoptosis by inhibiting Wnt/β-Catenin signaling.

Anticancer Agents Med Chem 2022 May 19. Epub 2022 May 19.

Department of Dermatology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.

Background: Melanoma is a highly invasive and metastatic malignant tumor originating from melanocytes and is associated with a poor prognosis. Surgical resection and chemotherapy are currently the main therapeutic options for malignant melanoma; however, their efficacy is poor, highlighting the need for the development of new, safe, and effective drugs for the treatment of this cancer.

Objective: To investigate the effects of alantolactone (ALT) on the proliferative, migratory, invasive, and apoptotic ability of malignant melanoma cells and explore its potential anticancer mechanism.

Methods: Melanoma cells (A375 and B16) were treated with different concentrations (4, 6, 8, and 10 μmol/L) of ALT, with DMSO and no treatment serving as controls. The effects of the different concentrations of the drug on cell proliferation were assessed by crystal violet staining and CCK-8 assay. The effects on cell migration and invasion were detected by wound healing and Transwell assays, respectively. Flow cytometry was used to evaluate the effects of the drug on apoptosis and the cell cycle. ALT target genes in melanoma were screened using network pharmacology. Western blotting was used to measure the expression levels of the proliferation-related protein PCNA; the apoptosis-related proteins Bax, Bcl-2, and caspase-3; the invasion and metastasis-related proteins MMP-2, MMP-7, MMP-9, vimentin, E-cadherin, and N-cadherin; and the canonical Wnt signaling pathway-related proteins β-catenin, c-Myc, and p-GSK3B. In addition, an l model of melanoma was established by the subcutaneous injection of A375 melanoma cells into nude mice, following which the effects of ALT treatment on malignant melanoma were determined in vivo.

Results: Compared with the controls, the proliferative, migratory, and invasive capacity of ALT-treated melanoma cells was significantly inhibited, whereas apoptosis was enhanced (P<0.01), showing effects that were exerted in a dose dependent manner. The expression levels of the pro-apoptotic proteins Bax and caspase-3, as well as those of the interstitial marker E-cadherin, were upregulated in melanoma cells irrespective of the ALT concentration (P<0.05). In contrast, the expression levels of the anti-apoptotic protein Bcl-2, the proliferation-related protein PCNA, and the invasion and metastasis-related proteins MMP-2, MMP-7, MMP-9, N-cadherin, and vimentin were downregulated (P<0.05). The network pharmacology results indicated that GSK3B may be a key ALT target in melanoma. Meanwhile, western blotting assays showed that ALT treatment markedly suppressed the expression of β-catenin as well as that of its downstream effector c-Myc, and could also inhibit GSK3B phosphorylation.

Conclusion: ALT can effectively inhibit the culture viability, migration, and invasion of A375 and B16 melanoma cells while also promoting their apoptosis. ALT may exert its anti-melanoma effects by inhibiting the Wnt/β-catenin signaling pathway. Combined, our data indicate that ALT has potential as an effective and safe therapeutic drug for the treatment of melanoma.
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http://dx.doi.org/10.2174/1871520622666220519100054DOI Listing
May 2022

A new species of beaded lacewings (Neuroptera, Berothidae) from mid-Cretaceous Myanmar amber.

Zookeys 2022 4;1092:93-104. Epub 2022 Apr 4.

School of Earth Sciences and Key Laboratory of Mineral Resources in Western China (Gansu Province), Lanzhou University, Lanzhou 730000, China Lanzhou University Lanzhou China.

A new species of Berothidae, sp. nov., is described and illustrated from mid-Cretaceous (lowest Cenomanian) Myanmar amber. It is easily distinguished from other species of Berothidae by the configuration of the wing venation including: forewing with distinct areas of infuscation surrounding cross-veins and vein forks, all cross-veins simple prior to ScP-RA fusion, presence of two cross-veins ra-rp; absence of inner or outer graduate series of cross-veins; RP with three branches; and absence of ma-mp cross-veins and cua-cup cross-veins; while hind wing has cross-vein 1r-m absent. The previous diagnoses of Grimaldi, 2000 and Grimaldi, 2000 are quite unclear because some characters occur mosaically in both genera. In order to solve this problem and distinguish from other species in the family, a new key to species of Berothidae from Myanmar amber has been provided and the diagnoses of and have been revised.
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http://dx.doi.org/10.3897/zookeys.1092.79396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005459PMC
April 2022

Dual-resolving of positional and geometric isomers of C=C bonds via bifunctional photocycloaddition-photoisomerization reaction system.

Nat Commun 2022 05 12;13(1):2652. Epub 2022 May 12.

The Institute for Advanced Studies, Wuhan University, Wuhan, Hubei, 430072, China.

The biological functions of lipids largely depend on their chemical structures. The position and configuration of C=C bonds are two of the essential attributes that determine the structures of unsaturated lipids. However, simultaneous identification of both attributes remains challenging. Here, we develop a bifunctional visible-light-activated photocycloaddition-photoisomerization reaction system, which enables the dual-resolving of the positional and geometric isomerism of C=C bonds in lipids when combines with liquid chromatography-mass spectrometry. The dual-pathway reaction mechanism is demonstrated by experiments and density functional theory calculations. Based on this bifunctional reaction system, a workflow of deep structural lipidomics is established, and allows the revealing of unique patterns of cis-trans-isomers in bacteria, as well as the tracking of C=C positional isomers changes in mouse brain ischemia. This study not only offers a powerful tool for deep lipid structural biology, but also provides a paradigm for developing the multifunctional visible-light-induced reaction.
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http://dx.doi.org/10.1038/s41467-022-30249-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098869PMC
May 2022

Deubiquitinating enzyme USP9X regulates metastasis and chemoresistance in triple-negative breast cancer by stabilizing Snail1.

J Cell Physiol 2022 07 3;237(7):2992-3000. Epub 2022 May 3.

College of Pharmacy, Jinan University, Guangzhou, Guangdong, China.

Breast cancer is one of the most common malignancies in women worldwide. Triple-negative breast cancer (TNBC) is a highly aggressive and metastatic subtype that has the characteristics of easy recurrence, poor prognosis as well as lack of targeted therapeutics. Snail1, a key factor regulating epithelial-mesenchymal transition (EMT) process, contributing to metastasis and chemoresistance in human cancers. However, the molecular mechanism of Snail1 stabilization in cancers is not fully understood. Here, we demonstrate that the deubiquitinating enzyme USP9X deubiquitinates and stabilizes Snail1, thereby promoting metastasis and chemoresistance. The depletion and pharmacological inhibition of USP9X by WP1130, an inhibitor of USP9X, downregulate endogenous Snail1 protein, inhibit cell migration, invasion, metastasis, and increase cellular sensitivity to cisplatin and paclitaxel both in vitro and in vivo, whereas the reconstitution of Snail1 in cells with USP9X depletion at least partially reverses these phenotypes. Overall, our study establishes the USP9X-Snail1 axis as an important regulatory mechanism of breast cancer metastasis and chemoresistance and provides a rationale for potential therapeutic interventions in the treatment of TNBC.
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http://dx.doi.org/10.1002/jcp.30763DOI Listing
July 2022

A novel and facile synthesis strategy for highly stable cesium lead halide nanowires.

RSC Adv 2021 Aug 25;11(46):28716-28722. Epub 2021 Aug 25.

Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes, National Laboratory of Mineral Materials, School of Materials Science and Technology, China University of Geosciences (Beijing) 100083 Beijing P. R. China.

As promising low-dimensional semiconductor materials, cesium lead halide (CsPbX, X = Cl, Br, I) perovskite-like nanowires (NWs) can be widely applied to the field of semiconductor devices and integrated optoelectronics. Therefore, developing a facile and efficient synthesis method of cesium lead halide perovskite-like NWs can bring both fundamental and practical impacts to the field of optoelectronics. Here, we developed a synthesis strategy of all-inorganic cesium lead halide CsPbI perovskite-like NWs under catalyst-free, solution-phase, and low-temperature conditions. The synthesis strategy was designed such that no inert gas is required and thus enables the synthesis to be carried out in air, which significantly reduces temperature, steps, time, and cost required for the reaction. The as-synthesized NWs were 7 μm in length and 80-100 nm in diameter with ideal morphology. Most of the CsPbI NWs were crystallized in orthorhombic phases that were arranged orderly with a uniform growth direction. In addition, the CsPbI NWs showed a photoluminescence peak near 610 nm and the fluorescence lifetime was 7.34 ns. The photoluminescence mechanism of CsPbI NWs involves the self-trapping behaviour in the radiative recombination process. The composition of CsPbI NWs is highly related to the synthesis temperature. The facile synthesis strategy has opened up a novel path for the synthesis of perovskite-like NWs, laying the foundation for the application of nano-optoelectronic devices, fluorescent anti-counterfeiting, and fluorescent composite materials.
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http://dx.doi.org/10.1039/d1ra04429jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038128PMC
August 2021

Impact of sulfhydryl ligands on the transformation of silver ions by molybdenum disulfide and their combined toxicity to freshwater algae.

J Hazard Mater 2022 08 21;435:128953. Epub 2022 Apr 21.

Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education)/Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China.

The transformation of silver ions (Ag) mediated by engineered nanomaterials (ENMs) influences the biosafety of Ag-containing products in natural environments. Actually, modification of biomolecules to ENMs in aquatic ecosystems alters their interactions with Ag. This study discovered that surface functionalization of glutathione (GSH, a sulfhydryl compound ubiquitous in natural waters) on molybdenum disulfide (MoS) nanoflakes suppressed the redox reaction between 1 T components and Ag, inhibiting the MoS-mediated reduction of Ag to Ag nanoparticles (AgNPs) in aqueous phase in the dark. However, AgNPs formation (from 2.32 ± 0.35-3.25 ± 0.29 mg/L per day, pH 7.0) and oxidation of MoS were remarkably accelerated after GSH binding under light conditions. The dominant electron donator of MoS to Ag was transformed from the electron-hole pairs to surface ligands driven by the introduction of chromophoric groups was authenticated as the cause for the elevated Ag reduction. These processes also occurred between Ag and MoS at low levels (50 μg/L). Additionally, the joint algal toxicity of GSH-modified MoS with Ag was weaker than that of pristine MoS due to increased retention of free Ag and AgNPs formation. Our findings improve the understanding of the interaction between ENMs and Ag in aquatic ecosystems.
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http://dx.doi.org/10.1016/j.jhazmat.2022.128953DOI Listing
August 2022

CX3CL1 inhibits NLRP3 inflammasome-induced microglial pyroptosis and improves neuronal function in mice with experimentally-induced ischemic stroke.

Life Sci 2022 Jul 22;300:120564. Epub 2022 Apr 22.

Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Department of Anesthesiology, Institute of Anesthesia and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. Electronic address:

Aims: Stroke represents the second cause of mortality across the globe and develops following the interruption of cerebral blood circulation. The chemokine CX3CL1 and its receptor CX3CR1 play a fundamental role in the pathophysiology of ischemic stroke. In this study, we investigated the protective effect of CX3CL1 against cerebral ischemia both in vitro and in vivo.

Main Methods: We employed an in vivo mice model of middle cerebral artery occlusion(MCAO)/reperfusion and in vitro BV2 cells model of oxygen-glucose deprivation/re‑oxygenation (OGD/R). Exogenous recombinant CX3CL1 (rCX3CL1) was administered into the lateral ventricle 1, 3 and 5 day(s) after reperfusion or in cell supernatant following OGD/R. Immunostaining, immunoblotting, and ELISA were performed to assess the NLRP3 inflammasome-induced pyroptosis both in vivo and in vitro. In addition, neurological deficits and infarct volume in mice were evaluated after MCAO.

Key Findings: The expression of CX3CL1 was downregulated after MCAO. Exogenous rCX3CL1 significantly reduced neurological deficits and infarct lesion in mice after MCAO. Moreover, exogenous rCX3CL1 inhibited GSDMD-dependent pyroptosis in microglia. Those effects further diminished NLRP3 inflammasome and NF-κB signaling activation, and also inhibited IL-1β and IL-18 expression both in vitro and in vivo.

Significance: These results demonstrated that exogenous rCX3CL1 administration after the ischemic insult exerted a long-term neuroprotective effect on post-ischemic stroke. And exogenous rCX3CL1 could inhibit NLRP3 inflammasome-induced microglial pyroptosis under ischemic conditions. Collectively, our findings showed that CX3CL1 signaling pathway can serve as a therapeutic target for promoting the functional recovery after stroke.
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http://dx.doi.org/10.1016/j.lfs.2022.120564DOI Listing
July 2022

γδ T Cell in Cerebral Ischemic Stroke: Characteristic, Immunity-Inflammatory Role, and Therapy.

Front Neurol 2022 31;13:842212. Epub 2022 Mar 31.

Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Gamma-delta (γδ) T cells are a small subset of T cells that are reported to have a proinflammatory role in the pathophysiology of cerebral ischemia stroke (CIS). Upon activation by interleukin-1 beta (IL-1β), IL-23 and IL-18, γδ T cells are stimulated to secrete various cytokines, such as IL-17a, IL-21, IL-22, and interferon-gamma (IFN-γ). In addition, they all play a pivotal role in the inflammatory and immune responses in ischemia. Nevertheless, the exact mechanisms responsible for γδ T cell proinflammatory functions remain poorly understood, and more effective therapies targeting at γδ T cells and cytokines they release remain to be explored, particularly in the context of CIS. CIS is the second most common cause of death and the major cause of permanent disability in adults worldwide. In this review, we focus on the neuroinflammatory and immune functions of γδ T cells and related cytokines, intending to understand their roles in CIS, which may be crucial for the development of novel effective clinical applications.
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http://dx.doi.org/10.3389/fneur.2022.842212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008352PMC
March 2022

An enhanced immunochromatography assay based on colloidal gold-decorated polydopamine for rapid and sensitive determination of gentamicin in animal-derived food.

Food Chem 2022 Sep 7;387:132916. Epub 2022 Apr 7.

Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China. Electronic address:

In this study, an enhancing immunochromatography assay (ICA) based on colloidal gold-decorated polydopamine ([email protected]) was firstly developed for gentamicin in milk, muscle, liver and kidney simultaneously. [email protected] was synthesized by one-pot method with better signal intensity, colloidal stability, and antibody coupling efficiency than traditional colloidal gold. The detection limit of the developed ICA was about 1.50 μg/kg for the four animal-derived food, which was up to 92-fold more sensitive than the reported ICA based on colloidal gold. The recovery rates were ranged from 86.0% to 114.0% with coefficient of variation between 1.6% and 13.1%. Parallel analysis of 40 samples by commercial ELISA kits was confirmed the reliability. Our research indicated that polydopamine decorated can chemically modify the surface of colloidal gold and can thus remarkably improve the detection performances of ICA.
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http://dx.doi.org/10.1016/j.foodchem.2022.132916DOI Listing
September 2022

In Situ Synthesis of Silver Nanoparticles on Flame-Retardant Cotton Textiles Treated with Biological Phytic Acid and Antibacterial Activity.

Materials (Basel) 2022 Mar 30;15(7). Epub 2022 Mar 30.

School of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China.

Fabrics were flame-retardant finished using phytic acid, a cost-effective, ecologically acceptable, and easily available flame-retardant finishing chemical. Then, on the surface of the completed fabric, silver nanoparticles (Ag NPs) were grown in situ to minimize Ag NPs aggregation and heterogeneous post-finishing and to increase washing durability. Thus, flame-retardant and antibacterial qualities were added to textiles. The as-prepared textiles were evaluated for their combustion performance, thermal performance, and antibacterial capabilities. At the same time, their microstructures were studied using X-ray diffractometry (XRD), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS). The findings indicated that flame-retardant textiles had an excellent launderability (limiting oxygen index = 31% after 20 washing cycles). Meanwhile, Ag NPs-loaded flame-retardant textiles demonstrated self-extinguishing properties, with a limiting oxygen index (LOI) of 27%. Bacteriostatic widths of flame-retardant antibacterial textiles against and were 5.28 and 4.32 mm, respectively, indicating that Ag NPs-loaded flame-retardant fabrics have certain flame-retardant and antibacterial capabilities. SEM and TEM analysis indicated that nanoparticles were uniformly dispersed over Ag NPs-loaded flame-retardant textiles and were around 20 nm in size. When compared to flame-retardant textiles, Ag NPs-loaded flame-retardant fabrics showed varied binding energy of P and N on the surface and Ag ion emergence. Thermogravimetric analysis at various heating rates revealed that the main pyrolysis temperature range of flame-retardant fabrics decreased, while the main pyrolysis temperature range of Ag NPs-loaded flame-retardant fabrics increased; the heating rate influenced the pyrolysis range but not the fabric mass loss. In situ reduction synthesis of Ag NPs-loaded flame-retardant textiles may successfully reduce agglomeration and heterogeneous dispersion of nano-materials during post-finishing.
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http://dx.doi.org/10.3390/ma15072537DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000066PMC
March 2022

Retinal microglia: Functions and diseases.

Immunology 2022 Jul 22;166(3):268-286. Epub 2022 Apr 22.

The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Microglia, the resident immune cells in the retina and nervous system, make irreplaceable contributions to the maintenance of normal homeostasis and immune surveillance of these systems. Recently, great progress has been made in determining the origin, distribution, features and functions of retinal microglia and in identifying their roles in retinal diseases. In the retinal microenvironment, microglia constantly monitor changes in their surroundings and maintain balanced functions by communicating with other retinal cells. When disturbed, activated microglia may kill degenerated neurons and photoreceptors through phagocytosis and exacerbate retinal injury by producing multiple proinflammatory mediators. Numerous animal studies and in situ analyses of human tissue have shown that retinal microglia are involved in multiple retinal diseases. The functions and mechanisms of activated microglia in retinal disorders are gradually being elucidated. Increasing evidence points towards the dual roles of microglia in the retina and they are regulated by many factors. How to inhibit the detrimental effects of microglia and promote beneficial effects are worth studying. This review focuses primarily on the features and functions of microglia and how they participate in retinal diseases based on existing research findings. We also discuss current opinions about microglial transdifferentiation.
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http://dx.doi.org/10.1111/imm.13479DOI Listing
July 2022

Globular C1q domain-containing protein from Pinctada fucata martensii participates in the immune defense process.

Fish Shellfish Immunol 2022 Apr 29;123:521-527. Epub 2022 Mar 29.

Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang, 524088, China. Electronic address:

The globular C1q domain-containing (C1qDC) protein can recognize a variety of ligands, such as pathogen-associated molecular patterns, and plays an important role in the innate immune response. Our previous studies showed that a novel globular C1q domain-containing protein (PmC1qDC-1) is involved in the damage repair process of pearl oyster shells. However, the function of PmC1qDC-1 in pearl oyster innate immunity remains unknown. In the present study, the high-level structural analysis showed that PmC1qDC-1 was a spherical structure composed of 10 strands and was similar to the AiC1qDC-2 of bay scallop (Argopecten irradians). In situ hybridization indicated that PmC1qDC-1 had strong fluorescence signal in gills. Furthermore, the mRNA expression of PmC1qDC-1 was highly induced at 6-48 h in gill after lipopolysaccharide, peptidoglycan and polyinosinic-polycytidylic acid stimulation. Additionally, we obtained the recombinant protein of PmC1qDC-1 (rPmC1qDC-1) and found that rPmC1qDC-1 had antibacterial activity against Gram-negative (i.e., Pseudomonas aeruginosa, Vibrio parahaemolyticus, Escherichia coli, and Aeromonas hydrophila) and Gram-positive (i.e., Staphylococcus aureus and Bacillus subtilis) bacteria. These results indicated that PmC1qDC-1 might play an important role in the immune response against bacteria and viruses. This study provides clues for further studying the immune defense of Pinctada fucata martensii against pathogens and exploring the evolution of the classic pathway of complement system.
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http://dx.doi.org/10.1016/j.fsi.2022.02.048DOI Listing
April 2022
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