Publications by authors named "Jiawei Zeng"

25 Publications

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COVID-19 mRNA vaccines.

J Genet Genomics 2021 Mar 15. Epub 2021 Mar 15.

CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 101408, China. Electronic address:

The ongoing COVID-19 pandemic and its unprecedented global societal and economic disruptive impact highlight the urgent need for safe and effective vaccines. Taking substantial advantages of versatility and rapid development, two mRNA vaccines against COVID-19 have completed late-stage clinical assessment at an unprecedented speed and reported positive results. In this review, we outline keynotes in mRNA vaccine development, discuss recently published data on COVID-19 mRNA vaccine candidates, focusing on those in clinical trials and analyze future potential challenges.
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http://dx.doi.org/10.1016/j.jgg.2021.02.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959685PMC
March 2021

Preparation and Characterization of Graphene from Refined Benzene Extracted from Low-Rank Coal: Based on the CVD Technology.

Molecules 2021 Mar 28;26(7). Epub 2021 Mar 28.

CCTEG Shengyang Reserach Institute, Shengyang 110000, China.

Industrial preparation of graphene has been a research hotspot in recent years. Finding an economical and practical carbon source and reducing the cost of production and instrument is significant in industrial graphene production. Coal is a common carbon source. Efficient improvement and utilization in the cleaning of coal has recently been a popular research area. In this study, we developed a set of graphene preparation methods based on Anhui Huainan's low-rank gas coal (HNGC). Using self-built experimental equipment, benzene precursor was prepared from HNGC and used as carbon source to realize graphene growth. The quality of the graphene was characterized by a high-resolution microscope and Raman spectrometer. This study provides a new idea and method for the preparation of low-rank coal-based graphene.
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http://dx.doi.org/10.3390/molecules26071900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037014PMC
March 2021

Therapeutic Drug Monitoring and Pharmacokinetic Analysis of Cyclosporine in a Pediatric Patient with Hemophagocytic Lymphohistiocytosis Complicated by Diabetes Insipidus: A Grand Round.

Ther Drug Monit 2021 06;43(3):303-306

Department of Pharmacy, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.

Background: Hemophagocytic lymphohistiocytosis (HLH) is a rare life-threatening disease. Initial therapy is based on etoposide, dexamethasone, and cyclosporine (CSA). The pharmacokinetics (PKs) of CSA and other drugs are sometimes altered in patients with HLH complicated by diabetes insipidus (DI) but the precise mechanisms remain unknown.

Methods: In this study, the authors present a case of a 4-year-old boy with HLH complicated by DI. CSA concentrations were determined by enzyme multiplied immunoassay technique; noncompartmental PK analysis of the plasma concentration-time data was performed using PKSolver; and linear regression analysis was performed to determine linearity of relationship between urine output and C0 levels of CSA.

Results: Although C0 values of CSA were lower than the target levels, the patient was successfully treated and a good clinical outcome was achieved. Linear regression analysis showed a strong negative correlation between urine output and the serum trough concentration (C0) of CSA, pharmacokinetic analysis showed the main PK parameters of CSA as follows: C0, 50.2 mcg/L; peak concentration (Cmax), 723.4 mcg/L; area under the curve0-24, 7478.2 mcg·h/L; clearance, 0.77 L/h/kg, elimination half-life, 5.3 hours, and volume of distribution, 6.0 L/kg.

Conclusions: To the best of the authors' knowledge, this is the first report of the CSA PK profile in a patient with HLH complicated by DI. The authors suppose that a large fluid output and input leads to extensive CSA distribution. These results suggest that the monitoring of the Cmax and area under the curve of CSA might be more clinically and pharmacokinetically significant than that of C0 in patients with HLH complicated by DI. This case highlights the importance of therapeutic drug monitoring and demonstrates PK parameters of CSA in a pediatric patient with HLH complicated by DI.
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http://dx.doi.org/10.1097/FTD.0000000000000875DOI Listing
June 2021

Tumor promoting effects of circRNA_001287 on renal cell carcinoma through miR-144-targeted CEP55.

J Exp Clin Cancer Res 2020 Dec 1;39(1):269. Epub 2020 Dec 1.

College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Sichuan Province, 610075, Chengdu, PR China.

Background: Renal cell carcinoma (RCC) is a common urological cancer. circular RNAs (circRNAs) is involved in the development of various types of cancers. However, the roles and underlying mechanisms of circRNAs in RCC are not fully elucidated. Herein, we aimed to examine the potential effect of circ_001287 on RCC progression.

Materials And Methods: Microarray-based gene expression profiling of RCC was initially employed in order to identify differentially expressed genes. Next, the expression of circ_001287 was examined, and the cell line with the highest circ_001287 expression was selected for subsequent investigation. The interaction among circ_001287, miR-144, and CEP55 was identified by conducting luciferase reporter assay, RNA-pull down, RIP, RT-qPCR and FISH. The effect of circ_001287 on proliferative, invasive and migratory capacities as well as tumorigenicity of transfected cells in mice was examined using gain- and loss-of-function experiments.

Results: circ_001287 and CEP55 were highly expressed while miR-144 was decreased in RCC tissues and cell lines. circ_001287 can up-regulate CEP55 by binding to miR-144, which resulted in increased proliferative, invasive and migratory capacities and tumor growth in vivo. In addition, down-regulation of miR-144 was also observed to promote these biological activities.

Conclusions: Overall, these results elucidate a new mechanism for circ_001287 in RCC development and provide a potential therapeutic target for RCC patients.
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http://dx.doi.org/10.1186/s13046-020-01744-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706056PMC
December 2020

Overexpression of Rhophilin Rho GTPase-binding protein 2 promotes hepatocellular carcinoma.

Oncol Lett 2020 Dec 23;20(6):382. Epub 2020 Oct 23.

Department of Laboratory Medicine, Mianyang Central Hospital, Mianyang, Sichuan 621099, P.R. China.

Hepatocellular carcinoma is a serious public health problem in China. The mortality rate associated with the majority of cancer types has decreased as a result of targeted therapy. However, the mortality rates associated with hepatocellular carcinoma have not improved; therefore, the identification of new molecular targets is required for the development of novel targeted therapies. In the present study, a new molecular target, Rhophilin Rho GTPase-binding protein 2 (RHPN2), was identified. The levels of RHPN2 protein in tumor tissues were assessed via immunohistochemistry, while the mRNA levels were analyzed via reverse transcription-quantitative PCR. Additionally, cell viability was tested via MTT analysis. RHPN2 expression was upregulated in hepatocellular carcinoma tissues compared with that of matched adjacent normal tissues. More importantly, low expression of RHPN2 in patients with hepatocellular carcinoma was associated with an improved prognosis rate compared with patients with high expression. Downregulation of RHPN2 reduced the proliferation of hepatocellular carcinoma cells and increased the rate of apoptosis, whereas overexpression of RHPN2 demonstrated the opposite effects. Hepatocyte nuclear factor 1α was implicated in the mechanism of RHPN2. Overall, these data indicated that overexpression of RHPN2 may promote hepatocellular carcinoma.
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http://dx.doi.org/10.3892/ol.2020.12245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608026PMC
December 2020

Circular RNA circ_001842 plays an oncogenic role in renal cell carcinoma by disrupting microRNA-502-5p-mediated inhibition of SLC39A14.

J Cell Mol Med 2020 09 30;24(17):9712-9725. Epub 2020 Jul 30.

Department of Clinical Laboratory, Mianyang Central Hospital, Mianyang, China.

Renal cell carcinoma (RCC) is a common urologic malignancy, and up to 30% of RCC patients present with locally advanced or metastatic disease at the time of initial diagnosis. Increasing evidence suggests that circular RNAs (circRNAs) serve as genomic regulatory molecules in various human cancers. Our initial in silico microarray-based analysis identified that circRNA circ_001842 was highly expressed in RCC. Such up-regulation of circ_001842 in RCC was experimentally validated in tissues and cell lines using RT-qPCR. Thereafter, we attempted to identify the role of circ_001842 in the pathogenesis of RCC. Through a series of gain- and loss-of function assays, cell biological functions were examined using colony formation assay, Transwell assay, annexin V-FITC/PI-labelled flow cytometry and scratch test. A high expression of circ_001842 in tissues was observed as associated with poor prognosis of RCC patients. circ_001842 was found to elevate SLC39A14 expression by binding to miR-502-5p, consequently resulting in augmented RCC cell proliferation, migration and invasion, as well as EMT in vitro and tumour growth in vivo. These observations imply the involvement of circ_001842 in RCC pathogenesis through a miR-502-5p-dependent SLC39A14 mechanism, suggesting circ_001842 is a potential target for RCC treatment.
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http://dx.doi.org/10.1111/jcmm.15529DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520279PMC
September 2020

Mutated SASH1 promotes Mitf expression in a heterozygous mutated SASH1 knock‑in mouse model.

Int J Mol Med 2020 Sep 19;46(3):1118-1134. Epub 2020 Jun 19.

Clinical Research Center, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China.

The SAM and SH3 domain‑containing 1 (SASH1) genes have been identified as the causal genes of dyschromatosis universalis hereditaria (DUH); these genes cause the pathological phenotypes of DUH, and SASH1 variants have been shown to regulate the abnormal pigmentation phenotype in human skin in various genodermatoses. However, investigations into the mutated SASH1 gene have been limited to in vitro studies. In the present study, to recapitulate the molecular pathological phenotypes of individuals with DUH induced by SASH1 mutations, a heterozygous BALB/c mouse model, in which the human SASH1 c.1654 T>G (p. Tyr 551Asp, Y551D) mutation was knocked in was first generated. The in vivo functional experiments on Y551D SASH1 indicated that the increased expression of microphthalmia‑associated transcription factor (Mitf) was uniformly induced in the tails of heterozygous BALB/c mice, and an increased quantity of Mitf‑positive epithelial cells was also detected. An increased expression of Mitf‑ and Mitf‑positive cells was also demonstrated in the epithelial tissues of Y551D‑SASH1 affected individuals. In the present study, Mitf expression was also found to be increased by Y551D SASH1 in vitro. Taken together, these findings indicate that the upregulation of Mitf is the bona fide effector of the Y551D SASH1‑mediated melanogenesis signaling pathway in vivo. SASH1 may function as a scaffold molecule for the assembly of a SASH1‑Mitf molecular complex to regulate Mitf expression in the cell nucleus and thus to promote the hyperpigmented phenotype in the pathogenesis of DUH and other genodermatoses related to pigment abnormalities.
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http://dx.doi.org/10.3892/ijmm.2020.4652DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387086PMC
September 2020

lncRNA 00312 Attenuates Cell Proliferation and Invasion and Promotes Apoptosis in Renal Cell Carcinoma via miR-34a-5p/ASS1 Axis.

Oxid Med Cell Longev 2020 23;2020:5737289. Epub 2020 Mar 23.

Department of Clinical Laboratory, Mianyang Central Hospital, Affiliated to Southwest Medical University, Mianyang 621000, Sichuan Province, China.

Background: Previous studies have demonstrated that lncRNAs play functional roles in regulating cancer cell proliferation, invasion, and apoptosis. Recent studies confirmed that lncRNA 00312 has important biological functions in lung and colorectal cancer. However, the role of lncRNA 00312 in renal cell carcinoma (RCC) remains unclear. Our aim was to explore the function of lncRNA 00312 in RCC and its potential molecular mechanism.

Methods: RCC cell lines A498 and ACHN were used as models in this study. RT-PCR was performed to determine lncRNA 00312, miR-34a-5p, and ASS1 mRNA expression. Proliferation and invasion were examined by CCK-8 and Transwell assay to confirm the function role of lncRNA 00312. Western blot analysis was used to examine the expression of apoptotic proteins Bax and Bcl-2.

Results: lncRNA was significantly downregulated in RCC cells such as A498 and ACHN; the expression of lncRNA 00312 in RCC tissues was significantly lower than that in adjacent normal tissues. Patients with low expression of lncRNA 00312 have worse prognosis regarding pathological grade, tumor size, and TNM stage. Overexpression of lncRNA 00312 suppressed A498 and ACHN cell proliferation and invasion, while promoting apoptosis. Our study found that miR-34a-5p had the potential binding site with lncRNA 00312 and revealed the role of miR-34a-5p in RCC. Furthermore, we confirmed that lncRNA 00312 played its role with the participation of ASS1 and miR-34a-5p.

Conclusion: lncRNA 00312 can inhibit RCC proliferation and invasion and promote apoptosis by suppressing miR-34a-5p and overexpressing ASS1. Our study demonstrated that the lncRNA 00312/miR-34a-5p/ASS1 axis may play a functional role in the progression of RCC; lncRNA 00312 abundance is a prognostic factor candidate for RCC survival, which provides new insights for RCC clinical treatment. models in this study. RT-PCR was performed to determine lncRNA 00312, miR-34a-5p, and ASS1 mRNA expression. Proliferation and invasion were examined by CCK-8 and Transwell assay to confirm the function role of lncRNA 00312. Western blot analysis was used to examine the expression of apoptotic proteins Bax and Bcl-2.
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http://dx.doi.org/10.1155/2020/5737289DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140129PMC
December 2020

The effect of soy intervention on insulin-like growth factor 1 levels: A meta-analysis of clinical trials.

Phytother Res 2020 Jul 19;34(7):1570-1577. Epub 2020 Feb 19.

Department of Clinical Laboratory, Mianyang Central Hospital, Mianyang, China.

A low insulin-like growth factor 1 (IGF-1) level is known to be associated with many disorders. Several studies have shown that soy consumption may influence IGF-1, but the findings remain inconclusive. In this work, we conducted a systematic review and meta-analysis to provide a more accurate estimation of the effect of soy consumption on plasma IGF-1. A comprehensive systematic search was performed in Scopus, Embase, Web of Science, and PubMed/MEDLINE databases from inception until October 2019. Eight studies fulfilled the eligibility criteria. The pooled weighted mean difference (WMD) of the eligible studies was calculated with random-effects approach. Overall, a significant increment in plasma IGF-1 was observed following soy intervention (WMD: 13.5 ng/ml, 95% CI: 5.2, 21.8, I = 97%). Subgroup analyses demonstrated a significantly greater increase in IGF-1, when soy was administered at a dosage of ≤40 g/day (WMD: 11.7 ng/ml, 95% CI: 10.9 to 12.6, I = 98%), and when the intervention duration was <12 weeks (WMD: 26.6 ng/ml, 95% CI: 9.1 to 44.1, I = 0.0%). In addition, soy intervention resulted in a greater increase in IGF-1 among non-healthy subjects (WMD: 36 ng/ml, 95% CI: 32.7 to 39.4, I = 84%) than healthy subjects (WMD: 9.8 ng/ml, 95% CI: 8.9 to 10.7, I = 90%). In conclusion, this study provided the first meta-analytical evidence that soy intake may increase IGF-1 levels, but the magnitude of the increase is dependent on the intervention dosage, duration, and health status of the participants.
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http://dx.doi.org/10.1002/ptr.6630DOI Listing
July 2020

Control of locomotor speed, arousal, and hippocampal theta rhythms by the nucleus incertus.

Nat Commun 2020 01 14;11(1):262. Epub 2020 Jan 14.

School of Life Sciences, Tsinghua University, Beijing, 100084, China.

Navigation requires not only the execution of locomotor programs but also high arousal and real-time retrieval of spatial memory that is often associated with hippocampal theta oscillations. However, the neural circuits for coordinately controlling these important processes remain to be fully dissected. Here we show that the activity of the neuromedin B (NMB) neurons in the nucleus incertus (NI) is tightly correlated with mouse locomotor speed, arousal level, and hippocampal theta power. These processes are reversibly suppressed by optogenetic inhibition and rapidly promoted by optogenetic stimulation of NI NMB neurons. These neurons form reciprocal connections with several subcortical areas associated with arousal, theta oscillation, and premotor processing. Their projections to multiple downstream stations regulate locomotion and hippocampal theta, with the projection to the medial septum being particularly important for promoting arousal. Therefore, NI NMB neurons functionally impact the neural circuit for navigation control according to particular brains states.
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http://dx.doi.org/10.1038/s41467-019-14116-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959274PMC
January 2020

Single-cell transcriptomes and whole-brain projections of serotonin neurons in the mouse dorsal and median raphe nuclei.

Elife 2019 10 24;8. Epub 2019 Oct 24.

Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, United States.

Serotonin neurons of the dorsal and median raphe nuclei (DR, MR) collectively innervate the entire forebrain and midbrain, modulating diverse physiology and behavior. To gain a fundamental understanding of their molecular heterogeneity, we used plate-based single-cell RNA-sequencing to generate a comprehensive dataset comprising eleven transcriptomically distinct serotonin neuron clusters. Systematic in situ hybridization mapped specific clusters to the principal DR, caudal DR, or MR. These transcriptomic clusters differentially express a rich repertoire of neuropeptides, receptors, ion channels, and transcription factors. We generated novel intersectional viral-genetic tools to access specific subpopulations. Whole-brain axonal projection mapping revealed that DR serotonin neurons co-expressing vesicular glutamate transporter-3 preferentially innervate the cortex, whereas those co-expressing thyrotropin-releasing hormone innervate subcortical regions in particular the hypothalamus. Reconstruction of 50 individual DR serotonin neurons revealed diverse and segregated axonal projection patterns at the single-cell level. Together, these results provide a molecular foundation of the heterogenous serotonin neuronal phenotypes.
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http://dx.doi.org/10.7554/eLife.49424DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812963PMC
October 2019

SNX3 suppresses the migration and invasion of colorectal cancer cells by reversing epithelial-to-mesenchymal transition via the β-catenin pathway.

Oncol Lett 2019 Nov 12;18(5):5332-5340. Epub 2019 Sep 12.

Medical Research Center, The Third People's Hospital of Chengdu, The Second Chengdu Hospital Affiliated to Chongqing Medical University, The Affiliated Hospital of Southwest Jiaotong University, Chengdu, Sichuan 610036, P.R. China.

The Wnt/β-catenin signaling pathway is a well-studied pathway that drives the carcinogenesis and metastasis of colorectal cancer (CRC). The secretion of Wnt proteins is essential for the continuous activation of Wnt/β-catenin signaling in CRC. The secretion of wingless, which is homologous to the human Wnt protein, is mediated by sorting nexin 3 (SNX3) in ; however, the role of SNX3 in CRC remains unknown. In the present study it was demonstrated that SNX3 reduced the migratory and invasive ability of HCT116 human CRC cells, and reversed epithelial-mesenchymal transition (EMT). Conversely, in the HT29 CRC cell line, which endogenously expresses high levels of SNX3, short hairpin RNA or siRNA-mediated knockdown of SNX3 induced EMT, and enhanced cell migration and invasion. In addition, upregulation of SNX3 significantly inhibited metastasis of HCT116 cells to the lungs of mice. These SNX3-mediated effects were associated with downregulation of β-catenin. Taken together, by downregulating β-catenin, SNX3 may mediate EMT and reverse CRC metastasis.
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http://dx.doi.org/10.3892/ol.2019.10860DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6781754PMC
November 2019

Meta-analysis of clinical trials comparing the efficacy and safety of liposomal cisplatin versus conventional nonliposomal cisplatin in nonsmall cell lung cancer (NSCLC) and squamous cell carcinoma of the head and neck (SCCHN).

Medicine (Baltimore) 2018 Nov;97(46):e13169

Department of Clinical Laboratory, Mianyang Central Hospital, Mianyang.

Background: While liposomal cisplatin has shown enhanced drug tolerability and higher targeting property as compared with the conventional cisplatin, the doubt remains whether lipoplatin could improve its anticancer efficacy. What's more, there is still no systematic evaluation of the safety profiles of lipoplatin comparing with original cisplatin. Thus, we performed a systematic literature search for randomized clinical trials directly comparing efficacy and safety of liposomal cisplatin versus its conventional nonliposomal cisplatin.

Methods: The electronic search was conducted in PubMed, Embase, The Cochrane Library, and ClinicalTrials.gov from inception to February 10, 2018. The pooled odds ratio (OR) and 95% confidence intervals (CIs) of progressive disease (PD), partial response (PR), stable disease (SD), and adverse events (AEs) were obtained to assess the efficacy and safety. Heterogeneity was estimated using the I test (I > 50%, significant heterogeneity).

Results: The search yielded 5 clinical trials that meet inclusion criteria, with a total of 523 patients. We found that the liposome encapsulated cisplatin was more clinical efficacious than cisplatin as assessed by PD rate (OR, 0.46; 95% CI, 0.28-0.74; P = .002), while subgroup analysis of the only nonsmall cell lung cancer (NSCLC) patients showed higher response rates in PR (OR, 0.46; 95% CI, 0.28-0.74; P = .002) and PD (OR, 0.46; 95% CI, 0.28-0.74; P = .002) simultaneously. In addition, the toxicity meta-analysis revealed lipoplatin was much less toxic than the original cisplatin, with respect to grade 3 to 4 neurotoxicity (OR, 0.18; 95% CI, 0.04-0.74; P = .02), grade 3 to 4 leukopenia (OR, 0.47; 95% CI, 0.26-0.85; P = .01), grade 3 to 4 neutropenia (OR, 0.26; 95% CI, 0.09-0.71; P = .009), grade 1 and 2 nausea/vomiting (OR, 0.50; 95% CI, 0.32-0.77; P = .002), and grade 3 and 4 asthenia (OR, 0.11; 95% CI, 0.03-0.42; P = .001).

Conclusions: This meta-analysis revealed that with both NSCLC and squamous cell carcinoma of the head and neck (SCCHN) patients, liposomal cisplatin-based chemotherapy offers significant advantages regarding the PD and reduced toxicities relative to conventional cisplatin.
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http://dx.doi.org/10.1097/MD.0000000000013169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6257614PMC
November 2018

Electronic Systems Diagnosis Fault in Gasoline Engines Based on Multi-Information Fusion.

Sensors (Basel) 2018 Sep 3;18(9). Epub 2018 Sep 3.

Cummins East Asia Research & Development Co. Ltd., Wuhan 430070, China.

The rapid development of electronic techniques in automobile has led to an increase of potential safety hazards, thus, a strong on-board diagnostic (OBD) system is desperately needed. To solve the problem of OBD insensitivity to manufacture errors or aging faults, the paper proposes a novel multi information fusion method. The diagnostic model is composed of a data fusion layer, feature fusion layer, and decision fusion layer. They are based on the back propagation (BP) neural network, support vector machine (SVM), and evidence theory, respectively. Algorithms are mainly focused on the reliability allocation of diagnostic results, which come from the data fusion layer and feature fusion layer. A fault simulator system was developed to simulate bias and drift faults of the intake pressure sensor. The real vehicle experiment was carried out to acquire data that are used to verify the availability of the method. Diagnostic results show that the multi-information fusion method improves diagnostic accuracy and reliability effectively. The study will be a promising approach for the diagnosis bias and drift fault of sensors in electronic control systems.
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http://dx.doi.org/10.3390/s18092917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164238PMC
September 2018

Activation of persulfate by CuO-sludge-derived carbon dispersed on silicon carbide foams for odorous methyl mercaptan elimination: identification of reactive oxygen species.

Environ Sci Pollut Res Int 2020 Jan 28;27(2):1224-1233. Epub 2018 Aug 28.

Key Lab of Technology on Electrochemical Energy Storage and Power Generation in Guangdong Universities, School of Chemistry and Environment, South China Normal University, Guangzhou, 510006, China.

In this work, sludge-derived carbon (SC) was innovatively integrated with copper oxide (CuO) on macroporous silicon carbide foams to construct a distinctive catalyst (CuO/SC) with strong catalytic activity, which can effectively activate persulfate (PS) for the removal of methyl mercaptan (CHSH). The structure and morphology of CuO/SC were investigated by means of XRD, SEM, and EDS. The effects of initial pH values, copper contents, PS dosages, and flow rates on CHSH removal were also investigated. Under optimal condition, more than 90% of CHSH was removed by CuO/SC-PS combined system within 10-min reaction due to the synergistic function of CuO and SC. More importantly, on the basis of reactive species trapping and ESR spectroscopy, it is revealed that the responsible reactive species for catalytic CHSH composition were ·SO, ·OH, O, and ·O in CuO/SC-PS system. Finally, the possible PS activation scheme of CuO/SC samples was proposed.
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http://dx.doi.org/10.1007/s11356-018-3038-3DOI Listing
January 2020

Hypothalamic Circuits for Predation and Evasion.

Neuron 2018 02 1;97(4):911-924.e5. Epub 2018 Feb 1.

National Institute of Biological Sciences, Beijing 102206, China; School of Life Sciences, Tsinghua University, Beijing 100084, China; PTN Graduate Program, School of Life Sciences, Tsinghua University, Beijing 100084, China. Electronic address:

The interactions between predator and prey represent some of the most dramatic events in nature and constitute a matter of life and death for both sides. The hypothalamus has been implicated in driving predation and evasion; however, the exact hypothalamic neural circuits underlying these behaviors remain poorly defined. Here, we demonstrate that inhibitory and excitatory projections from the mouse lateral hypothalamus (LH) to the periaqueductal gray (PAG) in the midbrain drive, respectively, predation and evasion. LH GABA neurons were activated during predation. Optogenetically stimulating PAG-projecting LH GABA neurons drove strong predatory attack, and inhibiting these cells reversibly blocked predation. In contrast, LH glutamate neurons were activated during evasion. Stimulating PAG-projecting LH glutamate neurons drove evasion and inhibiting them impeded predictive evasion. Therefore, the seemingly opposite behaviors of predation and evasion are tightly regulated by two dissociable modular command systems within a single neural projection from the LH to the PAG. VIDEO ABSTRACT.
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http://dx.doi.org/10.1016/j.neuron.2018.01.005DOI Listing
February 2018

3D MnO hollow microspheres ozone-catalysis coupled with flat-plate membrane filtration for continuous removal of organic pollutants: Efficient heterogeneous catalytic system and membrane fouling control.

J Hazard Mater 2018 Feb 14;344:1198-1208. Epub 2017 Nov 14.

Key Lab of Technology on Electrochemical Energy Storage and Power Generation in Guangdong Universities, School of Chemistry and Environment, South China Normal University, Guangzhou, 510006, China. Electronic address:

A heterogeneous catalytic ozonation/membrane filtration (HCOMF) system was fabricated by integrating a flat-plate polyvinylidene fluoride (PVDF) membrane module along with a slurry catalytic ozonation reactor. The performance and catalytic activity of HCOMF was evaluated for degradation of model wastewater containing bisphenol A (BPA) and humid acid (HA) under different permeation flux in long-term continuous experiments. The membrane fouling was investigated by trans-membranous pressure (TMP), membrane filtration resistance, scanning electronic microscopy (SEM), and fluorescence spectra. The results showed that HCOMF system exhibited an excellent and stable catalytic activity in long-term continuous experiments owning to integration of 3D MnO hollow microsphere ozone-catalysis with flat-plate membrane filtration. The TMP of HCOMF system didn't increase significantly, and the membrane resistance R and R declined from 4% and 16% to 1% and 4%, respectively, thus, the membrane fouling of HCOMF system was mitigated compared to MF system. The mitigation of membrane fouling in HCOMF system was attributed to the increase of hydrophilicity of membrane surface and change of HA fractions.
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http://dx.doi.org/10.1016/j.jhazmat.2017.11.024DOI Listing
February 2018

Tigecycline treatment in an infant with extensively drug-resistant Acinetobacter baumannii bacteremia.

Int J Infect Dis 2017 Aug 29;61:23-26. Epub 2017 May 29.

Department of Pediatric Intensive Care Unit, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. Electronic address:

The successful use of tigecycline in a 12-month old liver transplant recipient with extensively drug-resistant Acinetobacter baumannii bacteremia is presented. Tigecycline serum concentrations were monitored to help improve antibiotic efficacy and minimize side effects. A literature review identified 11 additional pediatric cases of A. baumannii infection treated with tigecycline since 2011. Tigecycline treatment should be considered in children with extensively drug-resistant bacterial infections.
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http://dx.doi.org/10.1016/j.ijid.2017.05.013DOI Listing
August 2017

p53 regulates ERK1/2/CREB cascade via a novel SASH1/MAP2K2 crosstalk to induce hyperpigmentation.

J Cell Mol Med 2017 10 6;21(10):2465-2480. Epub 2017 Apr 6.

Children's Hospital and Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

We previously reported that three point mutations in SASH1 and mutated SASH1 promote melanocyte migration in dyschromatosis universalis hereditaria (DUH) and a novel p53/POMC/Gαs/SASH1 autoregulatory positive feedback loop is regulated by SASH1 mutations to induce pathological hyperpigmentation phenotype. However, the underlying mechanism of molecular regulation to cause this hyperpigmentation disorder still remains unclear. In this study, we aimed to investigate the molecular mechanism undergirding hyperpigmentation in the dyschromatosis disorder. Our results revealed that SASH1 binds with MAP2K2 and is induced by p53-POMC-MC1R signal cascade to enhance the phosphorylation level of ERK1/2 and CREB. Moreover, increase in phosphorylated ERK1/2 and CREB levels and melanogenesis-specific molecules is induced by mutated SASH1 alleles. Together, our results suggest that a novel SASH1/MAP2K2 crosstalk connects ERK1/2/CREB cascade with p53-POMC-MC1R cascade to cause hyperpigmentation phenotype of DUH.
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http://dx.doi.org/10.1111/jcmm.13168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618682PMC
October 2017

A novel P53/POMC/Gαs/SASH1 autoregulatory feedback loop activates mutated SASH1 to cause pathologic hyperpigmentation.

J Cell Mol Med 2017 04 25;21(4):802-815. Epub 2016 Nov 25.

Children's Hospital and Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

p53-Transcriptional-regulated proteins interact with a large number of other signal transduction pathways in the cell, and a number of positive and negative autoregulatory feedback loops act upon the p53 response. P53 directly controls the POMC/α-MSH productions induced by ultraviolet (UV) and is associated with UV-independent pathological pigmentation. When identifying the causative gene of dyschromatosis universalis hereditaria (DUH), we found three mutations encoding amino acid substitutions in the gene SAM and SH3 domain containing 1 (SASH1), and SASH1 was associated with guanine nucleotide-binding protein subunit-alpha isoforms short (Gαs). However, the pathological gene and pathological mechanism of DUH remain unknown for about 90 years. We demonstrate that SASH1 is physiologically induced by p53 upon UV stimulation and SASH and p53 is reciprocally induced at physiological and pathophysiological conditions. SASH1 is regulated by a novel p53/POMC/α-MSH/Gαs/SASH1 cascade to mediate melanogenesis. A novel p53/POMC/Gαs/SASH1 autoregulatory positive feedback loop is regulated by SASH1 mutations to induce pathological hyperpigmentation phenotype. Our study demonstrates that a novel p53/POMC/Gαs/SASH1 autoregulatory positive feedback loop is regulated by SASH1 mutations to induce pathological hyperpigmentation phenotype.
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http://dx.doi.org/10.1111/jcmm.13022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345616PMC
April 2017

Three-dimensional MnO porous hollow microspheres for enhanced activity as ozonation catalysts in degradation of bisphenol A.

J Hazard Mater 2017 Jan 5;321:162-172. Epub 2016 Sep 5.

Key Lab of Technology on Electrochemical Energy Storage and Power Generation in Guangdong Universities, School of Chemistry and Environment, South China Normal University, Guangzhou, 510006, China. Electronic address:

Three-dimensional (3D) MnO porous hollow microspheres (δ- and α- MnO PHMSs), with high adsorption and catalytic ozonation performance, were synthesized by a self-template (MnCO microspheres) process at room temperature. The synthesized MnO PHMSs were characterized by X-ray diffraction (XRD), scanning electronic microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and Brunauer-Emmett-Teller (BET) surface area. The results showed that PHMSs exhibit the excellent adsorption ability and catalytic activity owning to their hollow spherical structure, mesoporous shell and well-defined interior voids, leading to the strong adsorption for bisphenol A (BPA) and the retention of O molecules on catalyst. Moreover, the catalytic performance of α-MnO PHMSs was better than that of δ-MnO PHMSs which was attributed to the richer lattice oxygen of α-MnO PHMSs to accelerate O decomposition by producing more reactive oxidative species. The degradation efficiency of BPA using 3D α-MnO PHMSs was more than 90% in the presence of ozone within 30min reaction time. The probe tests for reactive oxidative species (ROSs) displayed that BPA degradation by catalytic ozonation is dominated by O and OH in our present study. Furthermore, the organic compounds as intermediates of the degradation process were identified by LC/MS.
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http://dx.doi.org/10.1016/j.jhazmat.2016.09.013DOI Listing
January 2017

Serotonin neurons in the dorsal raphe nucleus encode reward signals.

Nat Commun 2016 Jan 28;7:10503. Epub 2016 Jan 28.

Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.

The dorsal raphe nucleus (DRN) is involved in organizing reward-related behaviours; however, it remains unclear how genetically defined neurons in the DRN of a freely behaving animal respond to various natural rewards. Here we addressed this question using fibre photometry and single-unit recording from serotonin (5-HT) neurons and GABA neurons in the DRN of behaving mice. Rewards including sucrose, food, sex and social interaction rapidly activate 5-HT neurons, but aversive stimuli including quinine and footshock do not. Both expected and unexpected rewards activate 5-HT neurons. After mice learn to wait for sucrose delivery, most 5-HT neurons fire tonically during waiting and then phasically on reward acquisition. Finally, GABA neurons are activated by aversive stimuli but inhibited when mice seek rewards. Thus, DRN 5-HT neurons positively encode a wide range of reward signals during anticipatory and consummatory phases of reward responses. Moreover, GABA neurons play a complementary role in reward processing.
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http://dx.doi.org/10.1038/ncomms10503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4738365PMC
January 2016

Proteomic screening and identification of microRNA-128 targets in glioma cells.

Proteomics 2015 Aug 15;15(15):2602-17. Epub 2015 May 15.

The State Key Laboratory of Medical Molecular Biology, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P. R. China.

Brain-enriched miR-128 is repressed in glioma cells, and could inhibit the proliferation of gliomas by targeting genes such as E2F3a and BMI1. To identify more targets of miR-128 in glioblastoma multiforme, the pulse stable isotope labeling with amino acids in cell culture (pSILAC) technique was used to test its impact on whole protein synthesis in T98G glioma cells. We successfully identified 1897 proteins, of which 1459 proteins were quantified. Among them, 133 proteins were downregulated after the overexpression of miR-128. Through predictions using various bioinformatics tools, 13 candidate target genes were chosen. A luciferase assay validated that 11 of 13 selected genes were potential targets of miR-128, and a mutagenesis experiment confirmed CBFB, CORO1C, GLTP, HnRNPF, and TROVE2 as the target genes. Moreover, we observed that the expression of CORO1C, TROVE2, and HnRNPF were higher in glioma cell lines compared to normal brain tissues and presented a tendency toward downregulation after overexpression of miR-128 in T98G cells. Furthermore, we have validated that CORO1C, TROVE2, and HnRNPF could inhibit glioma cell proliferation. In sum, our data showed that the integration of pSILAC and bioinformatics analysis was an efficient method for seeking the targets of miRNAs, and plentiful targets of miR-128 were screened and laid the foundation for research into the miR-128 regulation network.
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http://dx.doi.org/10.1002/pmic.201400128DOI Listing
August 2015

New method for the visual detection of human respiratory syncytial virus using reverse transcription loop-mediated amplification.

J Virol Methods 2014 Sep 9;206:84-8. Epub 2014 Jun 9.

Pathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Science, Shanghai, China. Electronic address:

Human respiratory syncytial virus (HRSV) is a seasonal respiratory pathogen that causes respiratory infection in children and the elderly. A new, reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid (within 1h), simultaneous detection of A and B group HRSV. Primers specific for groups A and B were designed to amplify the N and L genes of HRSV, respectively. A fluorescent dye, calcein, was used as an indicator for the endpoint visual detection and/or real-time amplification of HRSV RNA. The detection limit of the new method was 281.17 50% tissue culture infective doses (TCID50)/ml for HRSV A and 1.58 TCID50/ml for HRSV B. To evaluate the validity of this method, a comparison with RT-PCR was performed using 77 nasopharyngeal swabs as samples. Both RT-LAMP and RT-PCR detected HRSV in 38 HRSV samples, yielding a positive rate of 49%. Of the RT-LAMP positive samples, 36 (95%) were also positive by RT-PCR, while two were negative by RT-PCR. Among the 36 RT-LAMP and RT-PCR positive samples, 11 belonged to HRSV group A, while 25 belonged to group B. The results show that the new RT-LAMP is simple, rapid and well suited for HRSV diagnosis, especially in a limited-resource setting.
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http://dx.doi.org/10.1016/j.jviromet.2014.06.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113655PMC
September 2014

Dorsal raphe neurons signal reward through 5-HT and glutamate.

Neuron 2014 Mar;81(6):1360-1374

National Institute of Biological Sciences, Beijing 102206, China; School of Life Sciences, Tsinghua University, Beijing 100084, China. Electronic address:

The dorsal raphe nucleus (DRN) in the midbrain is a key center for serotonin (5-hydroxytryptamine; 5-HT)-expressing neurons. Serotonergic neurons in the DRN have been theorized to encode punishment by opposing the reward signaling of dopamine neurons. Here, we show that DRN neurons encode reward, but not punishment, through 5-HT and glutamate. Optogenetic stimulation of DRN Pet-1 neurons reinforces mice to explore the stimulation-coupled spatial region, shifts sucrose preference, drives optical self-stimulation, and directs sensory discrimination learning. DRN Pet-1 neurons increase their firing activity during reward tasks, and this activation can be used to rapidly change neuronal activity patterns in the cortex. Although DRN Pet-1 neurons are often associated with 5-HT, they also release glutamate, and both neurotransmitters contribute to reward signaling. These experiments demonstrate the ability of DRN neurons to organize reward behaviors and might provide insights into the underlying mechanisms of learning facilitation and anhedonia treatment.
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http://dx.doi.org/10.1016/j.neuron.2014.02.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411946PMC
March 2014