Publications by authors named "Jiaqi Wang"

521 Publications

Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis.

J Vis Exp 2021 Jun 4(172). Epub 2021 Jun 4.

Department of Biochemistry and Molecular Biology, Saitama University; Department of Botany, University of Wisconsin;

Plants respond to mechanical stresses such as wounding and herbivory by inducing defense responses both in the damaged and in the distal undamaged parts. Upon wounding of a leaf, an increase in cytosolic calcium ion concentration (Ca signal) occurs at the wound site. This signal is rapidly transmitted to undamaged leaves, where defense responses are activated. Our recent research revealed that glutamate leaking from the wounded cells of the leaf into the apoplast around them serves as a wound signal. This glutamate activates glutamate receptor-like Ca permeable channels, which then leads to long-distance Ca signal propagation throughout the plant. The spatial and temporal characteristics of these events can be captured with real-time imaging of living plants expressing genetically encoded fluorescent biosensors. Here we introduce a plant-wide, real-time imaging method to monitor the dynamics of both the Ca signals and changes in apoplastic glutamate that occur in response to wounding. This approach uses a wide-field fluorescence microscope and transgenic Arabidopsis plants expressing Green Fluorescent Protein (GFP)-based Ca and glutamate biosensors. In addition, we present methodology to easily elicit wound-induced, glutamate-triggered rapid and long-distance Ca signal propagation. This protocol can also be applied to studies on other plant stresses to help investigate how plant systemic signaling might be involved in their signaling and response networks.
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http://dx.doi.org/10.3791/62114DOI Listing
June 2021

High-Voltage Flexible Aqueous Zn-Ion Battery with Extremely Low Dropout Voltage and Super-Flat Platform.

Nanomicro Lett 2020 Mar 19;12(1):75. Epub 2020 Mar 19.

State Key Laboratory of Advanced Welding and Joining, Harbin Institute of Technology, Shenzhen, 518055, People's Republic of China.

Flexible rechargeable aqueous zinc-ion batteries (ZIBs) have attracted extensive attentions in the energy storage field due to their high safety, environmental friendliness, and outstanding electrochemical performance while the exploration of high-voltage aqueous ZIBs with excellent rate capability is still a great challenge for the further application them in flexible and wearable electronics. Herein, we fabricated a 2.4 V high-voltage flexible aqueous ZIB, being among the highest voltage reported in aqueous ZIBs. Moreover, it exhibits extremely flat charging/discharging voltage platforms and the dropout voltage is only 0.1 V, which is the smallest gap in all aqueous batteries to our best knowledge. Furthermore, the prepared ZIB performs high rate capability of 25 C and energy density of 120 Wh kg and exhibits excellent safety under various destructive conditions including hammering, sewing, punching, and soaking. These extraordinary results indicate the great application potential of our high-voltage flexible aqueous ZIB in wearable electronics.
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http://dx.doi.org/10.1007/s40820-020-0414-6DOI Listing
March 2020

Integrating Real-World Evidence in the Regulatory Decision-Making Process: A Systematic Analysis of Experiences in the US, EU, and China Using a Logic Model.

Front Med (Lausanne) 2021 31;8:669509. Epub 2021 May 31.

State Key Laboratory in Quality Research of Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.

Real world evidence (RWE) and real-world data (RWD) are drawing ever-increasing attention in the pharmaceutical industry and drug regulatory authorities (DRAs) all over the world due to their paramount role in supporting drug development and regulatory decision making. However, there is little systematic documentary analysis about how RWE was integrated for the use by the DRAs in evaluating new treatment approaches and monitoring post-market safety. This study aimed to analyze and discuss the integration of RWE into regulatory decision-making process from the perspective of DRAs. Different development strategies to develop and adopt RWE by the DRAs in the US, Europe, and China were reviewed and compared, and the challenges encountered were discussed. It was found that different strategies on development of RWE were applied by FDA, EMA, and NMPA. The extent to which RWE was adopted in China was relatively limited compared to that in the US and EU, which was highly related to the national pharmaceutical environment and development stages. A better understanding of the overall goals, inputs, activities, outputs, and outcomes in developing RWE will help inform actions to harness RWD and leverage RWE for better health care decisions.
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http://dx.doi.org/10.3389/fmed.2021.669509DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200400PMC
May 2021

The combination of lactoferrin and linolenic acid inhibits colorectal tumor growth through activating AMPK/JNK-related apoptosis pathway.

PeerJ 2021 31;9:e11072. Epub 2021 May 31.

Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

Colorectal cancer is a common cause of death with few available therapeutic strategies, and the preventative complexes in adjunctive therapy are urgently needed. Increasing evidences have shown that natural ingredients, including lactoferrin, oleic acid, docosahexaenoic acid (DHA) and linolenic acid, possess anti-inflammatory and anti-tumor activities. However, investigations and comparisons of their combinations in colorectal tumor model have not been reported, and the mechanism is still unrevealed. In the study, we examined the viability, migration, invasion and apoptosis of HT29 cells to choose the proper doses of these components and to select the effective combination in vitro. BALB/c nude mice bearing colorectal tumor were used to explore the role of selected combination in inhibiting tumor development in vivo. Additionally, metabonomic detection was performed to screen out the specific changed metabolitesand related pathway. The results demonstrated that lactoferrin at 6.25 μM, oleic acid at 0.18 mM, DHA at 0.18 mM, and linolenic acid at 0.15 mM significantly inhibited the viabilities of HT29 cells ( < 0.05). The combination of lactoferrin (6.25 μM) + linolenic acid (0.15 mM) exhibited the strongest activity in inhibiting the migration and invasion of HT29 cells in vivo and suppressing tumor development in vitro ( < 0.05). Furthermore, the lactoferrin + linolenic acid combination activated p-AMPK and p-JNK, thereby inducing apoptosis of HT29 cells ( < 0.05). The present study was the first to show that lactoferrin + linolenic acid combination inhibited HT29 tumor formation by activating AMPK/JNK related pathway.
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http://dx.doi.org/10.7717/peerj.11072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8174148PMC
May 2021

Metabolomics analysis underlay mechanisms in the renal impairment of mice caused by combination of aflatoxin M1 and ochratoxin A.

Toxicology 2021 Jun 11:152835. Epub 2021 Jun 11.

Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China; Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China; Milk and Milk Products Inspection Center of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China. Electronic address:

Aflatoxin M1 (AFM1) and ochratoxin A (OTA) are pernicious mycotoxins widely co-existing in the environment. However, nephrotoxicity and underlying mechanism induced by AFM1 coupled with OTA still remain to be explored. In this study, CD-1 mice were treated with 3.5 mg/kg b.w. AFM1, OTA, and AFM1 + OTA for 35 days, and UPLC-MS-based metabolomics method was effectuated to investigate metabolomic profiles of mice kidney. Subsequent experiments on human renal proximal tubular (HK-2) cells were performed to dig out the causal connections between distinguished differential metabolites and nephrotoxicity. Compared with DMSO vehicle group, all three toxin treatments (AFM1 and OTA alone, and in combination) significantly reduced final body weight, and remarkably elevated the concentration of serum creatinine (SCr) and caused abnormal histological phenotypes (shown by histopathological slices). OTA, AFM1 + OTA but not AFM1 reduced the relative weight index of kidney. These phenotypic results indicated that AFM1 and OTA were both toxic to the body, and it seemed that OTA exhibited a notable impairment to kidney while AFM1 had similar but limited effect compared with OTA. Further metabolomics analysis showed that when AFM1 and OTA were combined together, OTA exerted dominant effect on the alteration of metabolic processes. There were few differences in the number of changed metabolites between OTA and AFM1 + OTA group. Among the differentially expressed metabolites affected by OTA, and AFM1 + OTA, lysophosphatidylcholines (LysoPCs) were identified as the main type with significant upregulation, in which LysoPC (16:0) accounted for the most prime proportion. Western blotting results of HK-2 cells showed that single OTA and AFM1 + OTA increased the apoptotic protein expressions of Bax, caspase 3 and PARP, and decreased the expression of Bcl-2; while AFM1 only raised the expression of caspase 3. LysoPC (16:0) but not LysoPC (18:1) lifted the protein level of caspase 3 and PARP in HK-2 cells, and reduced the level of Bcl-2. Taken together, this study is the first effort trying to assess nephrotoxicity of AFM1 with OTA, and we guessed that OTA had a more pronounced toxicity to kidney in contrast to AFM1. No obvious synergism between AFM1 and OTA was found to contribute to the occurrence or development of nephropathy. LysoPC (16:0) might be the pivotal metabolite in response to single OTA and combined AFM1 + OTA engendering renal injury.
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http://dx.doi.org/10.1016/j.tox.2021.152835DOI Listing
June 2021

Microbial interaction promotes desulfurization efficiency under high pH condition.

Environ Res 2021 Jun 10;200:111423. Epub 2021 Jun 10.

Department of Environmental Engineering, Zhejiang University, Hangzhou, China; Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Hangzhou, China; Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental Resource Sciences, Zhejiang University, Hangzhou, China. Electronic address:

The existence of HS in biogas may cause equipment corrosion and considerable SO emission. Commonly used biotrickling filters may cause biogas dilution or generation of explosive mixtures. Compared with biotrickling filters, two-step process such as bioscrubber filters can overcome these shortages. However, its removal efficiency was still limited due to low microbial activity under high pH condition. Here, a bioreactor filter was carried out under pH 9.0. Removal efficiency higher than 99% was achieved under sulfide loading rate reaching 4.24 kg S md. Results of network and high throughput sequencing showed that Thiobacillus acted as both dominant species (accounting for 75%) and unique kinless hub in this bioreactor. Other bacteria (accounting for 25%) contributed 75% to the network, which implied the intensive interaction between Thiobacillus and others. Sulfide removal ability and pH tolerance of pure bacteria and mixed culture were considered to verify how microbial interaction influenced them. Compared with pure bacteria, mixed culture had better performance under high pH condition, which confirmed that microbial interaction promoted desulfurization efficiency under high pH condition. These results showed that intensive microbial interaction might be the key to enhance sulfide removal efficiency under high pH condition.
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http://dx.doi.org/10.1016/j.envres.2021.111423DOI Listing
June 2021

Highly efficient reusable superhydrophobic sponge prepared by a facile, simple and cost effective biomimetic bonding method for oil absorption.

Sci Rep 2021 Jun 7;11(1):11960. Epub 2021 Jun 7.

School of Naval Architecture and Maritime, Zhejiang Ocean University, Zhoushan, 316022, China.

Superhydrophobic sponges have considerable potential for oil/water separation. Most of the methods used for superhydrophobic modification of sponges require toxic or harmful solvents, which have the drawbacks of hazardous to environment, expensive, and complex to utilize. Moreover, the hydrophobic layer on the surface of sponge is often easily destroyed. In this paper, a highly efficient superhydrophobic sponge with excellent reusability was developed by using a facile, simple and environmentally friendly dopamine biomimetic bonding method. Different types of sponges, such as melamine, polyethylene or polyurethane sponge wastes, were used as raw materials to prepare superhydrophobic sponges, which possess the advantages of inexpensive and abundant. The effects of different dopamine polymerization time and different hydrophobic agent dosage on the hydrophobicity and oil absorption capacity of melamine sponges were optimized. The study results showed that the water contact angle of the superhydrophobic sponge could reach 153° with excellent organic solvent absorption capacity of 165.9 g/g. Furthermore, the superhydrophobic sponge retained approximately 92.1% of its initial absorption capacity after 35 reutilization cycles. More importantly, the dopamine biomimetic bonding superhydrophobic modification method can be used for different types of sponges. Therefore, a universally applicable, facile, simple and environmentally friendly superhydrophobic modification method for sponges was developed.
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http://dx.doi.org/10.1038/s41598-021-91396-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185010PMC
June 2021

Laboratory trends, hyperinflammation, and clinical outcomes for patients with a systemic rheumatic disease admitted to hospital for COVID-19: a retrospective, comparative cohort study.

Lancet Rheumatol 2021 May 28. Epub 2021 May 28.

Division of Rheumatology, Inflammation, and Immunity, Brigham and Women's Hospital, Boston, MA, USA.

Background: COVID-19 can induce a hyperinflammatory state, which might lead to poor clinical outcomes. We aimed to assess whether patients with a systemic rheumatic disease might be at increased risk for hyperinflammation and respiratory failure from COVID-19.

Methods: We did a retrospective, comparative cohort study of patients aged 18 years or older admitted to hospital with PCR-confirmed COVID-19 at Mass General Brigham (Boston, USA). We identified patients by a search of electronic health records and matched patients with a systemic rheumatic disease 1:5 to comparators. We compared individual laboratory results by case status and extracted laboratory results and COVID-19 outcomes for each participant. We calculated the COVID-19-associated hyperinflammation score (cHIS), a composite of six domains (a score of ≥2 indicating hyperinflammation) and used logistic regression to estimate odds ratios (ORs) for COVID-19 outcomes by hyperinflammation and case status.

Findings: We identified 57 patients with a systemic rheumatic disease and 232 matched comparators who were admitted to hospital with COVID-19 between Jan 30 and July 7, 2020; 38 (67%) patients with a rheumatic disease were female compared with 158 (68%) matched comparators. Patients with a systemic rheumatic disease had higher peak median neutrophil-to-lymphocyte ratio (9·6 [IQR 6·4-22·2] 7·8 [4·5-16·5]; p=0·021), lactate dehydrogenase concentration (421 U/L [297-528] 345 U/L [254-479]; p=0·044), creatinine concentration (1·2 mg/dL [0·9-2·0] 1·0 mg/dL [0·8-1·4], p=0·014), and blood urea nitrogen concentration (31 mg/dL [15-61] 23 mg/dL [13-37]; p=0·033) than comparators, but median C-reactive protein concentration (149·4 mg/L [76·4-275·3] 116·3 mg/L [58·8-225·9]; p=0·11) was not significantly different. Patients with a systemic rheumatic disease had higher peak median cHIS than comparators (3 [1-5] 2 [1-4]; p=0·013). All patients with a peak cHIS of 2 or more had higher odds of admission to intensive care (OR 3·45 [95% CI 1·98-5·99]), mechanical ventilation (66·20 [8·98-487·80]), and in-hospital mortality (16·37 [4·75-56·38]) than patients with a peak cHIS of less than 2. In adjusted analyses, patients with a rheumatic disease had higher odds of admission to intensive care (2·08 [1·09-3·96]) and mechanical ventilation (2·60 [1·32-5·12]) than comparators, but not in-hospital mortality (1.78 [0·79-4·02]). Among patients who were discharged from hospital, risk of rehospitalisation (1·08 [0·37-3·16]) and mortality within 60 days (1·20 [0·58-2·47]) was similar in patients and comparators.

Interpretation: Patients with a systemic rheumatic disease who were admitted to hospital for COVID-19 had increased risk for hyperinflammation, kidney injury, admission to intensive care, and mechanical ventilation compared with matched comparators. However, among patients who survived, post-discharge outcomes were not significantly different. The cHIS identified patients with hyperinflammation, which was strongly associated with poor COVID-19 outcomes in both patients with a rheumatic disease and comparators. Clinicians should be aware that patients with systemic rheumatic diseases and COVID-19 could be susceptible to hyperinflammation and poor hospital outcomes.

Funding: None.
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http://dx.doi.org/10.1016/S2665-9913(21)00140-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163294PMC
May 2021

Effect of Exposure Rates with Customized versus Conventional Titanium Mesh on Guided Bone Regeneration: A Systematic Review and Meta-Analysis.

J Oral Implantol 2021 Jun 5. Epub 2021 Jun 5.

Beijing Citident Stomatology Hospital.

Titanium mesh exposure is the main complication of bone regeneration. In this study, a meta-analysis and performed to clarify the effect of customized titanium mesh versus conventional titanium mesh complications and the time of mesh exposure on edentulous alveolar ridge GBR. Databases, including PubMed, EMBASE, Web of Science and Cochrane Central Register Controlled Trials, were searched by two independent reviewers to retrieve articles published from January 2010 to March 2020, regarding the incidence of complications after GBR surgery, with language limited to English articles. A total of 705 articles were found, and 9 articles were quantitatively analyzed. A funnel plot was made for 10 comprehensive datasets. The combined value of the total exposure rate of titanium mesh was 0.44 (44%, 95% CI=0.30~0.58). The results of subgroup analysis showed that the combined value of the customized titanium mesh exposure rate was 0.31 (31%, 95% CI=0.15~0.51), and the combined value of the conventional titanium mesh exposure rate was 0.51 (51%, 95% CI=0.33~0.69). Based on the findings of the present study, the exposure rate of customized titanium mesh is lower than that of conventional titanium mesh. The design of 3D printing customized titanium mesh avoids nerves and blood vessels, which is of great significance to improve the accurate reconstruction of GBR and provides enough space for implantation and reducing the exposure rate. Soft tissue management (i.e., technical sensitivity) is also an important factor to avoid soft tissue fractures.
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http://dx.doi.org/10.1563/aaid-joi-D-20-00200DOI Listing
June 2021

Reduction of air pollutants and associated mortality during and after the COVID-19 lockdown in China: Impacts and implications.

Environ Res 2021 Jun 2;200:111457. Epub 2021 Jun 2.

Guangdong Provincial Institute of Public Health, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China; Department of Public Health, School of Medicine, Jinan University, Guangzhou, China. Electronic address:

Although strict lockdown measurements implemented during the COVID-19 pandemic have dramatically reduced the anthropogenic-based emissions, changes in air quality and its health impacts remain unclear in China. We comprehensively described air pollution during and after the lockdown periods in 2020 compared with 2018-2019, and estimated the mortality burden indicated by the number of deaths and years of life lost (YLL) related to the air pollution changes. The mean air quality index (AQI), PM, PM, NO, SO and CO concentrations during the lockdown across China declined by 18.2 (21.2%), 27.0 μg/m (28.9%), 10.5 μg/m (18.3%), 8.4 μg/m (44.2%), 13.1 μg/m (38.8%), and 0.3 mg/m (27.3%) respectively, when compared to the same periods during 2018-2019. We observed an increase in O concentration during the lockdown by 5.5 μg/m (10.4%), and a slight decrease after the lockdown by 3.4 μg/m (4.4%). As a result, there were 51.3 (95%CI: 32.2, 70.1) thousand fewer premature deaths (16.2 thousand during and 35.1 thousand after the lockdown), and 1066.8 (95%CI: 668.7, 1456.8) thousand fewer YLLs (343.3 thousand during and 723.5 thousand after the lockdown) than these in 2018-2019. Our findings suggest that the COVID-19 lockdown has caused substantial decreases in air pollutants except for O, and that substantial human health benefits can be achieved when strict control measures for air pollution are taken to reduce emissions from vehicles and industries. Stricter tailored policy solutions of air pollution are urgently needed in China and other countries, especially in well-developed industrial regions, such as upgrading industry structure and promoting green transportation.
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http://dx.doi.org/10.1016/j.envres.2021.111457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170876PMC
June 2021

The circular RNA circSPARC enhances the migration and proliferation of colorectal cancer by regulating the JAK/STAT pathway.

Mol Cancer 2021 Jun 1;20(1):81. Epub 2021 Jun 1.

Department of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221002, Jiangsu Province, China.

Background: Noncoding RNAs such as circular RNAs (circRNAs) are abundant in the human body and influence the occurrence and development of various diseases. However, the biological functions of circRNAs in colorectal cancer (CRC) are largely unknown.

Methods: RT-qPCR was used to detect the expression of circRNAs and mRNA in CRC cells and tissues. Fluorescence in situ hybridization (FISH) was used to analyze the location of circSPARC. Function-based experiments were performed using circSPARC knockdown and overexpression cell lines in vitro and in vivo, including CCK8, colony formation, transwell and metastasis models. Mechanistically, luciferase reporter assay, western blots, RNA immunoprecipitation (RIP), Chromatin isolation by RNA purification (ChIRP) and immunohistochemical stainings were performed.

Results: CircSPARC was upregulated in both the tissues and plasma of CRC patients. High expression of circSPARC was associated with advanced TNM stage, lymph node metastases, and poor survival. Silencing circSPARC inhibited CRC cell migration and proliferation in vitro and vivo. Mechanistically, circSPARC sponged miR-485-3p to upregulate JAK2 expression and ultimately contribute to the accumulation of phosphorylated (p)-STAT3. Besides, circSPARC recruited FUS, which facilitated the nuclear translocation of p-STAT3.

Conclusions: These findings suggest that circSPARC might serve as a potential diagnostic and prognostic biomarker and a therapeutic target for CRC treatment by regulating JAK2/STAT3 pathway.
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http://dx.doi.org/10.1186/s12943-021-01375-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167978PMC
June 2021

Easy domain adaptation method for filling the species gap in deep learning-based fruit detection.

Hortic Res 2021 Jun 1;8(1):119. Epub 2021 Jun 1.

International Field Phenomics Research Laboratory, Institute for Sustainable Agro-Ecosystem Services, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 188-0002, Japan.

Fruit detection and counting are essential tasks for horticulture research. With computer vision technology development, fruit detection techniques based on deep learning have been widely used in modern orchards. However, most deep learning-based fruit detection models are generated based on fully supervised approaches, which means a model trained with one domain species may not be transferred to another. There is always a need to recreate and label the relevant training dataset, but such a procedure is time-consuming and labor-intensive. This paper proposed a domain adaptation method that can transfer an existing model trained from one domain to a new domain without extra manual labeling. The method includes three main steps: transform the source fruit image (with labeled information) into the target fruit image (without labeled information) through the CycleGAN network; Automatically label the target fruit image by a pseudo-label process; Improve the labeling accuracy by a pseudo-label self-learning approach. Use a labeled orange image dataset as the source domain, unlabeled apple and tomato image dataset as the target domain, the performance of the proposed method from the perspective of fruit detection has been evaluated. Without manual labeling for target domain image, the mean average precision reached 87.5% for apple detection and 76.9% for tomato detection, which shows that the proposed method can potentially fill the species gap in deep learning-based fruit detection.
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http://dx.doi.org/10.1038/s41438-021-00553-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167097PMC
June 2021

Epigenetic regulation of intestinal peptide transporter PEPT1 as a potential strategy for colorectal cancer sensitization.

Cell Death Dis 2021 May 24;12(6):532. Epub 2021 May 24.

National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 518116, Shenzhen, China.

Human intestinal peptide transporter PEPT1 is commonly repressed in human colorectal cancer (CRC), yet its relationship with sensitivity to the common CRC treatment ubenimex has not previously been elucidated. In this study, we confirmed PEPT1 suppression in CRC using real-time quantitative polymerase chain reaction and western blotting and then investigated the underlying epigenetic pathways involved using bisulfite sequencing, chromatin immunoprecipitation, siRNA knockdown, and reporter gene assays. We found that PEPT1 transcriptional repression was due to both DNMT1-mediated DNA methylation of the proximal promoter region and HDAC1-mediated histone deacetylation, which blocked P300-mediated H3K18/27Ac at the PEPT1 distal promoter. Finally, the effects of the epigenetic activation of PEPT1 on the CRC response to ubenimex were evaluated using sequential combination therapy of decitabine and ubenimex both in vitro and in xenografts. In conclusion, epigenetic silencing of PEPT1 due to increased DNMT1 and HDAC1 expression plays a vital role in the poor response of CRC to ubenimex.
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http://dx.doi.org/10.1038/s41419-021-03814-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144210PMC
May 2021

Microbiota and Tuberculosis: A Potential Role of Probiotics, and Postbiotics.

Front Nutr 2021 7;8:626254. Epub 2021 May 7.

Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Institutes of Biomedical Sciences, Shanxi University, Taiyuan, China.

Tuberculosis (TB), caused by attacking the lungs and other organs, is one of the most common infectious disease worldwide. According to the WHO's 2020 report, a quarter of the world's population were infected with , and ~1.4 million people died of TB. Therefore, TB is a significant public health concern, which requires cost-effective strategies for prevention and treatment. The microbiota has been considered as a "forgotten organ" and a complex dynamic ecosystem, which plays a significant role in many physiological processes, and its dysbiosis is closely associated with infectious disease. Recently, a few studies have indicated associations between TB and microbiota. This review summarizes studies concerning the alterations of the gut and respiratory microbiota in TB, and their relationship with host susceptibility to infection, indicating that microbiota signatures in different stages in TB progression could be considered as biomarkers for TB diagnosis and control. In addition, the potential role of probiotics and postbiotics in TB treatment was discussed.
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http://dx.doi.org/10.3389/fnut.2021.626254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138307PMC
May 2021

LncRNA CPhar Induces Cardiac Physiological Hypertrophy and Promotes Functional Recovery After Myocardial Ischemia-Reperfusion Injury.

Circulation 2021 May 21. Epub 2021 May 21.

Shanghai Engineering Research Center of Organ Repair, School of Medicine, Shanghai University, Shanghai 200444, China; Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, School of Life Science, Shanghai University, Shanghai 200444, China.

Exercise training's benefits in cardiovascular system have been well accepted, however, the underlying mechanism remains to be explored. Here, we report the initial functional characterization of an exercise-induced cardiac physiological hypertrophy associated novel lncRNA. Using lncRNA microarray profiling, we identified lncRNAs in contributing the modulation of exercise-induced cardiac growth that we termed Cardiac Physiological hypertrophy associated regulator (CPhar). Mice with Adeno-associated virus serotype 9 (AAV9) driving CPhar overexpression and knockdown were used in experiments. Swim training was used to induce physiological cardiac hypertrophy in mice and ischemia reperfusion injury (IR/I) surgery was conducted to investigate the protective effects of CPhar in mice. To investigate the mechanisms of CPhar's function, we performed various analysis including RTqPCR, western blot, histology, cardiac function (by echocardiography), functional rescue experiments, mass spectrometry, RNA transcription, RNA pull down, RNA immunoprecipitation, chromatin immunoprecipitation assay, luciferase reporter assay, and coimmunoprecipitation assays. We screened the lncRNAs in contributing the modulation of exercise-induced cardiac growth via lncRNA microarray profiling and found that CPhar was increased with exercise and was necessary for exercise-induced physiological cardiac growth. Gain- and loss- of function of CPhar regulated the expression of proliferation markers, hypertrophy, and apoptosis in cultured neonatal mouse cardiomyocytes (NMCMs). Overexpression of CPhar prevented myocardial ischemia reperfusion injury and cardiac dysfunction . We identified DDX17 as a binding partner of CPhar in regulating CPhar downstream factor ATF7 by sequestering C/EBPβ. Our study of this lncRNA CPhar provides new insights into the regulation of exercise-induced cardiac physiological growth, demonstrating the cardioprotective role of CPhar in the heart, as well as expanding our mechanistic understanding of lncRNA function.
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http://dx.doi.org/10.1161/CIRCULATIONAHA.120.050446DOI Listing
May 2021

Design of Bacillus fastidious Uricase Mutants Bearing Long Lagging Phases Before Exponential Decreases of Activities Under Physiological Conditions.

Protein J 2021 May 20. Epub 2021 May 20.

Chongqing Key Laboratory of Medicinal Chemistry & Molecular Pharmacology, Chongqing University of Technology, Chongqing, 400054, China.

Under physiological conditions, Bacillus fastidious uricase (BFU) activity shows negligible lagging phase before the exponential decrease; mutants are thus designed for long lagging phases before exponential activity decreases. On homodimer surface of BFU (4R8X.pdb), the last fragment ANSEYVAL at the C-terminus forms a loop whose Y319 is H-bonded by the buried D257 in the same monomer. Within 1.5 nm from the α-carboxyl group of the last leucine (L322), E30, K26, D257, R258, E311, K312 and E318 from the same monomer plus D126 and K127 from a monomer of the other homodimer generate an electrostatic interaction network. Within 1.5 nm from Y319, D307 and R310 in the same monomer interact with ionized residues around the inter-chain β-sheet in the same homodimer. Mutagenesis of Y319R is designed to strengthen the original interactions and concomitantly generate new electrostatic attractions between homodimers. Under physiological conditions, the mutant V144A/Y319R showed an approximately 4 week lagging phase before the exponential activity decrease, an apparent half-life of activity nearly three folds of mutant V144A, but comparable activity. The introduction of ionizable residues into the C-terminus contacting the other homodimer for additional and/or stronger electrostatic attractions between homodimers may be a universal approach to thermostable mutants of uricases.
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http://dx.doi.org/10.1007/s10930-021-09999-0DOI Listing
May 2021

Ruminal microbiota-host interaction and its effect on nutrient metabolism.

Anim Nutr 2021 Mar 15;7(1):49-55. Epub 2020 Dec 15.

State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.

Rumen microbiota has a close and intensive interaction with the ruminants. Microbiota residing in the rumen digests and ferments plant organic matters into nutrients that are subsequently utilized by the host, making ruminants a unique group of animals that can convert plant materials indigestible by humans into high-quality animal protein as meat and milk. Many studies using meta-omics technologies have demonstrated the relationships between rumen microbiome and animal phenotypes associated with nutrient metabolism. Recently, the causality and physiological mechanisms underpinning the host-microbiota interactions have attracted tremendous research interest among researchers. This review discusses the host-microbiota interactions and the factors affecting these interactions in ruminants and provides a summary of the advances in research on animal husbandry. Understanding the microbiota composition, the functions of key bacteria, and the host-microbiota interaction is crucial for the development of knowledge-based strategies to enhance animal productivity and host health.
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http://dx.doi.org/10.1016/j.aninu.2020.12.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110878PMC
March 2021

Radiomics-Based Features for Prediction of Histological Subtypes in Central Lung Cancer.

Front Oncol 2021 29;11:658887. Epub 2021 Apr 29.

Department of Radiology, The First Hospital of China Medical University, Shenyang, China.

Objectives: To evaluate the effectiveness of radiomic features on classifying histological subtypes of central lung cancer in contrast-enhanced CT (CECT) images.

Materials And Methods: A total of 200 patients with radiologically defined central lung cancer were recruited. All patients underwent dual-phase chest CECT, and the histological subtypes (adenocarcinoma (ADC), squamous cell carcinoma (SCC), small cell lung cancer (SCLC)) were confirmed by histopathological samples. 107 features were used in five machine learning classifiers to perform the predictive analysis among three subtypes. Models were trained and validated in two conditions: using radiomic features alone, and combining clinical features with radiomic features. The performance of the classification models was evaluated by the area under the receiver operating characteristic curve (AUC).

Results: The highest AUCs in classifying ADC vs. SCC, ADC vs. SCLC, and SCC vs. SCLC were 0.879, 0.836, 0.783, respectively by using only radiomic features in a feedforward neural network.

Conclusion: Our study indicates that radiomic features based on the CECT images might be a promising tool for noninvasive prediction of histological subtypes in central lung cancer and the neural network classifier might be well-suited to this task.
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http://dx.doi.org/10.3389/fonc.2021.658887DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117140PMC
April 2021

Global Analysis the Potential Medicinal Substances of Shuangxia Decoction and the Process via Mass Spectrometry Technology.

Front Pharmacol 2021 30;12:654807. Epub 2021 Apr 30.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.

Shuangxia decoction is an effective traditional Chinese medicine formula for treating insomnia. Up to now, there has not been any report about the effective substances. An omics data processing method based on mass spectrometry technology is used to explore the chemical composition changes of Shuangxia decoction, the components absorbed into the blood and brain, and to explore the anti-insomnia mechanism based on molecular docking technology. Forty-nine chemical components in Shuangxia decoction have been identified, and 51 new components generated by co-decoction have been discovered. It was found that 7,404 compounds of Shuangxia decoction were absorbed into the blood. Forty kinds of known compounds were quickly identified, and 15 new compounds generated by co-decoction were also found to be absorbed into the blood. By using UPLC-MS/MS method, it was confirmed that 10 compounds were absorbed into the blood and 9 compounds were absorbed into the brain. Furthermore, it is found that rosmarinic acid is mainly distributed in the hypothalamus and striatum, and caffeic acid is mainly distributed in the hypothalamus, striatum, and hippocampus. Molecular docking results showed rosmarinic acid, danshensu, and HMLA with GABA receptor have excellent binding characteristics, even surpassing the proligand. Danshensu and HMLA with dopamine D receptor also showed good binding energy. Our findings will help to further confirm the mechanism of Shuangxia decoction for relieving insomnia, and we also establish a novel data processing method for supplementing the mechanism of the efficacy of other traditional Chinese medicine formula.
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http://dx.doi.org/10.3389/fphar.2021.654807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120809PMC
April 2021

Relationship between rheumatoid arthritis and pulmonary function measures on spirometry in the UK Biobank.

Arthritis Rheumatol 2021 May 13. Epub 2021 May 13.

Division of Rheumatology, Inflammation, and Immunity, Brigham and Women's Hospital, Boston, MA, USA.

Objective: We investigated the independent relationship of rheumatoid arthritis (RA) with type and severity of pulmonary patterns on spirometry compared to general population controls.

Methods: This cross-sectional study investigated the association of RA and pulmonary function measures on spirometry among subjects who had spirometry performed for research purposes in the UK Biobank. RA cases were identified by self-report and current DMARD/glucocorticoid use. General population controls denied RA. Outcomes included continuous % predicted forced expiratory volume in 1 second (FEV ) and forced vital capacity (FVC), type of spirometric pattern (restrictive or obstructive), and severity. We used multivariable regression to estimate the effects of RA cases versus controls, adjusting for age, sex, body mass index (BMI), and smoking status/pack-years.

Results: Among 350,776 analyzed subjects with spirometry performed (mean age 56.3 years, 55.8% female, and 45.5% ever smokers), we identified 2,008 cases of treated RA. In multivariable analyses, RA was associated with lower % predicted FEV (β -2.93, 95%CI -3.63,-2.24), % predicted FVC (β -2.08, 95%CI -2.72,-1.45), and FEV /FVC (β -0.008, 95%CI -0.010,-0.005) than controls. RA was associated with restrictive (OR 1.36, 95%CI 1.21,1.52) and obstructive (OR 1.21, 95%CI 1.07,1.37) patterns independent of confounders. RA had the strongest associations for severe restrictive and obstructive patterns.

Conclusion: RA was associated with increased odds of restrictive and obstructive patterns, and this relationship was not explained by confounders including smoking. In addition to restrictive lung disease, clinicians should also be aware that airflow obstruction may be a pulmonary manifestation of RA.
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http://dx.doi.org/10.1002/art.41791DOI Listing
May 2021

Incorporating Guanidinium as Perovskitizer-Cation of Two-Dimensional Metal Halide for Crystal-Array Photodetectors.

Chem Asian J 2021 May 11. Epub 2021 May 11.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, 350002, P. R. China.

Two-dimensional (2D) hybrid perovskites are recently emerging as a potential family of semiconductors for versatile optoelectronic applications. Currently, the "perovskitizer" moieties are rigidly limited to small-size cations, while few 2D metal-halides containing guanidinium cations inside perovskite cages have been studied for photodetection. Herein, we present a new 2D hybrid perovskite, (i-BA) (G)Pb I (where G is guanidinium and i-BA is isobutylammonium), which adopts a bilayered framework of {GPb I }. Single-crystal structure analyses disclose that G cations act as the perovskitizer, confined in the flexible perovskite cages formed by the distorted PbI octahedra. Such inorganic sheets are crucial to the superior semiconducting properties and optical bandgap, as verified by the density functional theory calculation. Furthermore, its planar crystal-array photodetector shows fascinating photoelectric performance, including a quite low dark current (∼4.6×10  A), a large current switching ratio (∼1.0×10 ), and a notable photo-responsivity of ∼0.72 A W , suggesting great potential of (i-BA) (G)Pb I for photodetection.
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http://dx.doi.org/10.1002/asia.202100425DOI Listing
May 2021

Drug-induced liver injury: Oltipraz and C2-ceramide intervene HNF-1α/GSTA1 expression via JNK signaling pathway.

J Appl Toxicol 2021 May 6. Epub 2021 May 6.

Department of Basic Veterinary Science, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.

Drug-induced liver injury (DILI) is a serious and frequently occurring issue in drug development. The c-Jun N-terminal kinase (JNK) signaling pathway plays an important role in many diseases; hepatocyte nuclear factor-1α (HNF-1α) and glutathione S-transferase A1 (GSTA1) are important in regulating liver-specific genes expressions and affecting drug metabolism. Oltipraz is used to treat liver cirrhosis by improving liver function, and C2-ceramide is a pro-apoptotic lipid that regulates multiple signaling pathways. In this study, we investigated the function of the JNK signaling pathway with HNF-1α and GSTA1 in a cellular model of DILI and whether oltipraz and C2-ceramide exert effects via the JNK pathway. The results showed that inhibiting JNK could ameliorate APAP-induced hepatocyte injury, reduced oxidative stress, suppressed JNK and c-Jun activation, and hepatocyte apoptosis. Meanwhile, the mRNA and protein expressions of HNF-1α and GSTA1 were increased significantly compared to control conditions. The effect of oltipraz (8 μmol/L) was similar to a JNK inhibitor and significantly increased HNF-1α/GSTA1 expression, but oltipraz combined with JNK inhibitor did not show a synergistic effect. Although C2-ceramide (8 μmol/L) aggravated hepatocyte injury and apoptosis, exacerbated oxidative stress, increased phosphorylation of JNK and c-Jun, and markedly decreased HNF-1α/GSTA1 expression, C2-ceramide combined with JNK inhibitor could partially alleviate these alterations. These results demonstrated that the JNK signaling pathway with HNF-1α/GSTA1 are involved in the process of DILI. Inhibiting JNK up-regulated HNF-1α and GSTA1 expressions which could attenuate hepatocyte injury. Oltipraz and C2-ceramide might affect the expression of HNF-1α/GSTA1 though JNK signaling.
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http://dx.doi.org/10.1002/jat.4181DOI Listing
May 2021

Guided suturing technique for midface lift through minimal temporal incision.

J Plast Reconstr Aesthet Surg 2021 Apr 8. Epub 2021 Apr 8.

Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Science, 33 Badachu Rd., Shijingshan District, Beijing 100144, China. Electronic address:

Background: The midface has been a difficult zone to manage in rejuvenating surgeries. The major challenge for midface lift is to achieve extensive dissection and elevate the composite tissues en bloc through minimal incisions.

Methods: In a total of 22 composite midface lift cases, a titanium cable wire was used. The wire was made into an elastic loop that guided three SMAS-suspending sutures out of the subcutaneous plane through a short incision at the temporal region. Then, each suture was fixated to the deep temporal fascia. Postoperative complications and the subjective assessment of the patients were evaluated.

Results: Surgeries were successfully commenced in all cases. Midface sagging, eye bags, and nasolabial folds all exhibited considerable improvements. Major complications such as nerve injury and alopecia were not observed. The patients were followed up for an average of 16.9 months. In general, 90% of the patients were satisfied with their outcome.

Conclusion: This guided suspension suturing technique effectuates a more convenient and efficient way to conduct multi-points anchorage of the composite malar flap. When properly executed, this technique allows the anchorage sutures to bite into the deeper layer of the SMAS that ensures firm suspensions in midface rejuvenation surgery.
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http://dx.doi.org/10.1016/j.bjps.2021.03.061DOI Listing
April 2021

Regulation of MRP4 Expression by circHIPK3 via Sponging miR-124-3p/miR-4524-5p in Hepatocellular Carcinoma.

Biomedicines 2021 Apr 30;9(5). Epub 2021 Apr 30.

Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, Cancer Center of Zhejiang University, Hangzhou 310058, China.

Multidrug resistance-associated protein 4 (MRP4), a member of the adenosine triphosphate (ATP) binding cassette transporter family, pumps various molecules out of the cell and is involved in cell communication and drug distribution. Several studies have reported the role of miRNAs in downregulating the expression of MRP4. However, regulation of MRP4 by circular RNA (circRNA) is yet to be elucidated. In this study, MRP4 was significantly upregulated in hepatocellular carcinoma (HCC) tissues compared to the adjacent noncancerous tissues. Computational prediction, luciferase reporter assay and miRNA transfection were used to investigate the interaction between miRNAs and MRP4. miR-124-3p and miR-4524-5p reduced the expression of MRP4 at the protein but not mRNA level. Circular RNA in vivo precipitation and luciferase reporter assays demonstrated that circHIPK3, as a competitive endogenous RNA, binds with miR-124-3p and miR-4524-5p. Further, knockdown of circHIPK3 resulted in downregulation of MRP4 protein, whereas cotransfection of circHIPK3-siRNA and miR-124-3p or miR-4524-5p inhibitors restored its expression. In conclusion, we report that miR-4524-5p downregulates the expression of MRP4 and circHIPK3 regulates MRP4 expression by sponging miR-124-3p and miR-4524-5p for the first time. Our results may provide novel insights into the prevention of MRP4-related proliferation and multiple drug resistance in HCC.
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http://dx.doi.org/10.3390/biomedicines9050497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147194PMC
April 2021

Expression levels and activation status of Yap splicing isoforms determine self-renewal and differentiation potential of embryonic stem cells.

Stem Cells 2021 May 2. Epub 2021 May 2.

Laboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing, People's Republic of China.

Yap is the key effector of Hippo signaling; however, its role in embryonic stem cells (ESCs) remains controversial. Here, we identify two Yap splicing isoforms (Yap472 and Yap488), which show equal expression levels but heterogeneous distribution in ESCs. Knockout (KO) of both isoforms reduces ESC self-renewal, accelerates pluripotency exit but arrests terminal differentiation, while overexpression of each isoform leads to the reverse phenotype. The effect of both Yap isoforms on self-renewal is Teads-dependent and mediated by c-Myc. Nonetheless, different isoforms are found to affect overlapping yet distinct genes, and confer different developmental potential to Yap-KO cells, with Yap472 exerting a more pronounced biological effect and being more essential for neuroectoderm differentiation. Constitutive activation of Yaps, particularly Yap472, dramatically upregulates p53 and Cdx2, inducing trophectoderm trans-differentiation even under self-renewal conditions. These findings reveal the combined roles of different Yap splicing isoforms and mechanisms in regulating self-renewal efficiency and differentiation potential of ESCs.
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http://dx.doi.org/10.1002/stem.3389DOI Listing
May 2021

Two-Dimensional Guanidine-Based Hybrid Perovskites with Strong Dichroism for Multiwavelength Polarization-Sensitive Detection.

Chemistry 2021 Apr 29. Epub 2021 Apr 29.

College of Chemical Engineering, Fuzhou University, Fuzhou, Fujian, 350116, P. R. China.

Two-dimensional (2D) organic-inorganic hybrid perovskites, benefiting from their natural anisotropy of quantum-well motifs and optical properties, have shown remarkable polarization-dependent responses superior to the 3D counterparts. Here, for the first time, multiwavelength polarization-sensitive detectors were fabricated by using single crystals of a guanidine-based 2D hybrid perovskite, (BA) (GA)Pb I (where BA is n-butylammonium and GA is guanidium). Its unique 2D quantum-well structure results in strong crystallographic-dependence of optical absorption. Strikingly, our crystal-based photodetector exhibits a prominent photocurrent dichroic ratio (I /I ) of ∼2.2 at 520 nm, higher than the typical 2D inorganic materials (GeSe, ∼1.09, PdSe , ∼1.8). In addition, notable dichroic ratios of 1.29 and 1.23 at 405 nm and 637 nm are also created for the multiwavelength polarized-light detection. The prominent detecting performances, including low dark current (1.6×10  A), considerable on/off ratio (∼2×10 ), high photodetectivity (∼3.3×10 Jones) and responsivity (∼12.01 mA W ), make (BA) (GA)Pb I a promising candidate for polarized-light detection. This work sheds light on the rational engineering of new 2D hybrid perovskites for the high-performance optoelectronic device applications.
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http://dx.doi.org/10.1002/chem.202100691DOI Listing
April 2021

Effect of Food Endotoxin on Infant Health.

Toxins (Basel) 2021 04 22;13(5). Epub 2021 Apr 22.

State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Endotoxin is a complex molecule derived from the outer membrane of Gram-negative bacteria, and it has strong thermal stability. The processing of infant food can kill pathogenic bacteria but cannot remove endotoxin. Because the intestinal structure of infants is not fully developed, residual endotoxin poses a threat to their health by damaging the intestinal flora and inducing intestinal inflammation, obesity, and sepsis, among others. This paper discusses the sources and contents of endotoxin in infant food and methods for preventing endotoxin from harming infants. However, there is no clear evidence that endotoxin levels in infant food cause significant immune symptoms or even diseases in infants. However, in order to improve the safety level of infant food and reduce the endotoxin content, this issue should not be ignored. The purpose of this review is to provide a theoretical basis for manufacturers and consumers to understand the possible harm of endotoxin content in infant formula milk powder and to explore how to reduce its level in infant formula milk powder. Generally, producers should focus on cleaning the milk source, securing the cold chain, avoiding long-distance transportation, and shortening the storage time of raw milk to reduce the level of bacteria and endotoxin. After production and processing, the endotoxin content should be measured as an important index to test the quality of infant formula milk powder so as to provide high-quality infant products for the healthy growth of newborns.
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http://dx.doi.org/10.3390/toxins13050298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143472PMC
April 2021

Tribo-Induced Smart Reflector for Ultrasensitive Self-Powered Wireless Sensing of Air Flow.

ACS Appl Mater Interfaces 2021 May 29;13(18):21450-21458. Epub 2021 Apr 29.

Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.

Air-flow sensing is essential in broad applications of weather forecasting, ocean monitoring, gas leakage alarming, and health monitoring. However, in severe environments where electrical power supply and cable connection are not available, the sensing of air flow in a self-powered way is a challenging issue. In this work, we reported a tribo-induced smart reflector to achieve the self-powered wireless sensing of the air flow by combining an aerodynamics-driven triboelectric nanogenerator (TENG) and a silver-coated polymer network liquid crystal. Upon being driven by the air flow, the developed reflector performed specular and diffused reflectance without and with charging by the TENG, respectively, enabling wireless sensing through mechanical-electrical-optical signal conversion. In the developed sensing paradigm, the sensing module can be fully self-powered without the need of signal pre-amplification, which is electrically separated from the light source and detection modules without cable connections. The applications of self-powered wireless wind speed sensing and breath monitoring were performed to demonstrate the effectiveness of the developed paradigm toward self-powered wireless sensing nodes in the internet of things.
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http://dx.doi.org/10.1021/acsami.1c04048DOI Listing
May 2021

MBNL1 Suppressed Cancer Metastatic of Skin Squamous Cell Carcinoma Via by TIAL1/MYOD1/Caspase-9/3 Signaling Pathways.

Technol Cancer Res Treat 2021 Jan-Dec;20:1533033820960755

Department of Pathology, Wuhan Central Hospital, Huazhong University of Science and Technology, Wuhan, Hubei Province, China.

Objective: The incidence of skin squamous cell carcinoma (SSCC) has recently been increasing, with diverse clinical manifestations.SSCC could metastasize to lymph nodes or other organs, posing a great threat to life. The present study was designed to investigate the function and underlying mechanism of muscleblind-like protein 1 (MBNL1) in skin squamous cell carcinoma.

Methods: SCL-1 cell was used for vitro model and transfected with MBNL1 or siMBNL1 plasmids. MTT Assays, LDH activity ELISA, and Transwell chamber migration experiment were used to confirm the effects of MBNL1 on cell growth of SCL-1 cell. Western blot analysis was used to analyze the mechanism of MBNL1 in SCL-1 cell.

Results: Down-regulation of MBNL1 promoted cell metastasis of SSCC, while up-regulation of MBNL1 reduced cell metastasis of SSCC in vitro. Down-regulation of MBNL1 suppressed the protein expression of T cell intracellular antigen (TIAL1), myogenic determinant 1 (MyoD1) and Caspase-3 in vitro. Consistent with these observations, inhibition of TIAL1 or MYOD1 expression attenuated the effects of MBNL1 in SSCC.

Conclusion: The present study revealed that MBNL1 suppressed thecancer metastatic capacity of SSCC via by TIAL1/MYOD1/Caspase-3 signaling pathways.
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http://dx.doi.org/10.1177/1533033820960755DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085367PMC
April 2021

CARAFE++: Unified Content-Aware ReAssembly of FEatures.

IEEE Trans Pattern Anal Mach Intell 2021 Apr 21;PP. Epub 2021 Apr 21.

Feature reassembly, i.e., feature downsampling and upsampling, is a key operation in a number of modern convolutional network architectures, e.g., residual networks and feature pyramids. Its design is critical for dense prediction tasks such as object detection and semantic/instance segmentation. In this work, we propose unified Content-Aware ReAssembly of FEatures (CARAFE++), a universal, lightweight, and highly effective operator to fulfill this goal. CARAFE++ has several appealing properties: (1) Unlike conventional methods such as pooling and interpolation that only exploit sub-pixel neighborhood, CARAFE++ aggregates contextual information within a large receptive field. (2) Instead of using a fixed kernel for all samples (e.g., convolution and deconvolution), CARAFE++ generates adaptive kernels on-the-fly to enable instance-specific content-aware handling. (3) CARAFE++ introduces little computational overhead and can be readily integrated into modern network architectures. We conduct comprehensive evaluations on standard benchmarks in object detection, instance/semantic segmentation, and image inpainting. CARAFE++ shows consistent and substantial gains on mainstream methods across all the tasks with negligible computational overhead. It shows great potential to serve as a strong building block for modern deep networks.
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http://dx.doi.org/10.1109/TPAMI.2021.3074370DOI Listing
April 2021