Publications by authors named "Jiaqi Lv"

10 Publications

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The role of the cholinergic anti-inflammatory pathway in autoimmune rheumatic diseases.

Scand J Immunol 2021 Oct 1;94(4):e13092. Epub 2021 Aug 1.

Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Taiyuan, China.

The cholinergic anti-inflammatory pathway (CAP) is a classic neuroimmune pathway, consisting of the vagus nerve, acetylcholine (ACh)-the pivotal neurotransmitter of the vagus nerve-and its receptors. This pathway can activate and regulate the activities of immune cells, inhibit cell proliferation and differentiation, as well as suppress cytokine release, thereby playing an anti-inflammatory role, and widely involved in the occurrence and development of various diseases; recent studies have demonstrated that the CAP may be a new target for the treatment of autoimmune rheumatic diseases. In this review, we will summarize the latest progress with the view of figuring out the role of the cholinergic pathway and how it interacts with inflammatory reactions in several autoimmune rheumatic diseases, and many advances are results from a wide range of experiments performed in vitro and in vivo.
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http://dx.doi.org/10.1111/sji.13092DOI Listing
October 2021

Photoelectrochemical sensing and mechanism investigation of hydrogen peroxide using a pristine hematite nanoarrays.

Talanta 2022 Jan 2;237:122894. Epub 2021 Oct 2.

Henan Joint International Research Laboratory of Environmental Pollution Control Materials, Henan Provincial Engineering Research Center of Green Anticorrosion Technology for Magnesium Alloys, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, 475004, China. Electronic address:

In this paper, a facile hydrothermal combined with subsequent two-step post-calcination method was used to fabricate hematite (α-FeO) nanoarrays on fluorine-doped SnO glass (FTO). The morphology, crystalline phase, optical property and surface chemical states of the fabricated α-FeO photoelectrode were characterized by scanning electron microscopy, X-ray diffraction, ultraviolet visible spectroscopy and X-ray photoelectron spectroscopy correspondingly. The α-FeO photoelectrode exhibits excellent photoelectrochemical (PEC) response toward hydrogen peroxide (HO) in aqueous solutions, with a low detection limit of 20 μM (S/N = 3) and wide linear range (0.01-0.09, 0.3-4, and 6-16 mM). Additionally, the α-FeO photoelectrode shows satisfying reproducibility, stability, selectivity and good feasibility for real samples. Mechanism analysis indicates, comparing with HO, HO possesses much more fast reaction kinetics over α-FeO surface, thus the recombination of photogenerated charges are reduced, followed by much more photogenerated electrons are migrated to the counter electrode via external circuit. The insight on the enhanced photocurrent, which is corelative to the concentration of HO in aqueous solution, will stimulate us to further optimize the surface structure of α-FeO to gain highly efficient α-FeO based sensors.
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http://dx.doi.org/10.1016/j.talanta.2021.122894DOI Listing
January 2022

Encapsulated Mixture of Methyl Salicylate and Tributyrin Modulates Intestinal Microbiota and Improves Growth Performance of Weaned Piglets.

Microorganisms 2021 Jun 21;9(6). Epub 2021 Jun 21.

The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, China.

Tributyrin and essential oils have been used as alternatives to antimicrobials to improve gut health and growth performance in piglets. This study was to evaluate the effects of a dietary supplement with two encapsulated products containing different combinations of tributyrin with oregano or with methyl salicylate on growth performance, serum biochemical parameters related to the physiological status, intestinal microbiota and metabolites of piglets. A total of 108 weaned crossbred piglets (Yorkshire × Landrace, 21 ± 1 d, 8.21 ± 0.04 kg) were randomly divided into three groups. Piglets were fed with one of the following diets for 5 weeks: a basal diet as the control (CON); the control diet supplemented with an encapsulated mixture containing 30% of methyl salicylate and tributyrin at a dosage of 3 kg/t (CMT); and the control diet supplemented with an encapsulated mixture containing 30% of oregano oil and tributyrin at a dosage of 3 kg/t (COT). At the end of the feeding trial, six piglets from each group were slaughtered to collect blood and gut samples for physiological status and gut microbiological analysis. The study found that the CMT group was larger in feed intake (FI) ( < 0.05), average daily gain (ADG) ( = 0.09), total protein (TP), albumin (ALB), glutathione peroxidase (GSH-PX) ( < 0.05), blood total antioxidant capacity (T-AOC) ( < 0.05), and crypt depth in the ileum ( < 0.05) compared with the CON group. The genus abundance of and in the CMT group was significantly decreased compared with the CON group. The CMT group also resulted in significantly higher activity in amino acid metabolism and arginine biosynthesis compared with the CON group. The COT group was larger in T-AOC, and the genus abundance of and was significantly increased in the ileum compared with the CON group. Data analysis found a significantly high correlation between the genus abundance of and that of in the ileum. The genus abundance of was also positively correlated with the sorbitol level. In general, the results indicated that the supplementation of both encapsulated mixtures in diet of weaned piglets could improve the animal blood antioxidant capacity. Additionally, the encapsulated mixture of methyl salicylate plus tributyrin improved the growth performance and resulted in certain corresponding changes in nutrient metabolism and in the genus abundance of ileum microbial community.
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http://dx.doi.org/10.3390/microorganisms9061342DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235159PMC
June 2021

The main mechanisms of trimethyltin chloride-induced neurotoxicity: Energy metabolism disorder and peroxidation damage.

Toxicol Lett 2021 Jul 15;345:67-76. Epub 2021 Apr 15.

Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou, 510300, China. Electronic address:

Trimethyltin chloride (TMT) is a by-product in the synthesis of organotin, a plastic stabilizer. With the rapid development of industry, the occupational hazards caused by TMT cannot be ignored. TMT is a typical neurotoxicant, which mainly damages the limbic system and brainstem of the nervous system. Previous studies have demonstrated that the neurotoxicity induced by TMT is linked to the inhibition of energy metabolism, but the underlying mechanism remains elusive. In order to investigate the mechanism of TMT-induced inhibition of energy metabolism, C57BL/6 male mice were administered by IP injection in different TMT doses (0 mg/kg, 1.00 mg/kg, 2.15 mg/kg and 4.64 mg/kg) and times (1d, 3d and 6d), and then the changes of superoxide dismutase (SOD) activity, malondialdehyde (MDA) level and Na-K-ATPase activity in cerebral cortex, cerebellum, hippocampus, pons, medulla oblongata of mice, the expressions of Na-K-ATPase protein, AMP-activated protein kinase (AMPK), phosphorylated AMP-activated protein kinase(p-AMPK)and peroxisome proliferator-activated receptor γ coactivator-1 α (PGC-1α) in hippocampus and medulla oblongata were measured; the effects of TMT on the viability, the activity of SOD, glutathione (GSH) and Na-K-ATPase, MDA level, and the expression of PGC-1α and Na-K-ATPase protein in N2a cells were measured by different TMT doses and times, in order to verify the experiments in vivo. Our results found that most of the mice showed depression, tremor, epilepsy, spasm and other symptoms after TMT exposure. Moreover, with the increase of TMT dose, the activity of Na-K-ATPase and the expressions of AMPK protein in the hippocampus and medulla oblongata of mice decreased, and the expressions of p-AMPK protein increased. Peroxidative damage was evident in hippocampus, medulla oblongata of mice and N2a cells, and the expression of PGC-1α and Na-K-ATPase protein was significantly down-regulated. Therefore, it is reasonable to believe that TMT-induced neurotoxic symptoms and inhibition of energy metabolism may be related to p-AMPK and down-regulation of PGC-1α in the hippocampus and medulla oblongata.
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http://dx.doi.org/10.1016/j.toxlet.2021.04.008DOI Listing
July 2021

Multilabel Ranking with Inconsistent Rankers.

IEEE Trans Pattern Anal Mach Intell 2021 Apr 2;PP. Epub 2021 Apr 2.

While most existing multilabel ranking methods assume the availability of a single objective label ranking for each instance in the training set, this paper deals with a more common case where only subjective inconsistent rankings from multiple rankers are associated with each instance. Two ranking methods are proposed from the perspective of instances and rankers, respectively. The first method, Instance-oriented Preference Distribution Learning (IPDL), is to learn a latent preference distribution for each instance. IPDL generates a common preference distribution that is most compatible to all the personal rankings, and then learns a mapping from the instances to the preference distributions. The second method, Ranker-oriented Preference Distribution Learning (RPDL), is proposed by leveraging interpersonal inconsistency among rankers, to learn a unified model from personal preference distribution models of all rankers. These two methods are applied to natural scene images database and 3D facial expression database BU 3DFE. Experimental results show that IPDL and RPDL can effectively incorporate the information given by the inconsistent rankers, and perform remarkably better than the compared state-of-the-art multilabel ranking algorithms.
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http://dx.doi.org/10.1109/TPAMI.2021.3070709DOI Listing
April 2021

Study of Langerhans cells and T lymphocytes in vulvar lichen sclerosus lesions.

Australas J Dermatol 2021 May 16;62(2):e217-e222. Epub 2020 Dec 16.

Department of Dermatology, Beijing Hospital, National Center of Gerontology, Beijing, China.

Background: The aetiology and pathogenesis of vulvar lichen sclerosus (LS), a chronic inflammatory disease, is not completely clear. It has been found that local cellular immune abnormalities play an important role in the immune mechanism of LS, mainly characterised by abnormal numbers of Langerhans cells in the epidermis and abnormal numbers of dermal T lymphocytes.

Objective: To evaluate the densities of Langerhans cells and T-lymphocyte subpopulations in vulvar LS.

Methods: The density of Langerhans cells in the epidermis, and CD3 , CD4 and CD8 T cells in the dermis of seven early-stage and eight late-stage cases of vulvar LS were detected with direct immunofluorescence, and compared with 15 normal controls.

Results: The density of Langerhans cells in the late-stage group was significantly higher than in the normal group (P = 0.001). The densities of CD3 , CD4 and CD8 T lymphocytes in both the early- and late-stage (deeper dermis) groups were higher than in the normal group (P < 0.05). The ratio of CD4 /CD8 T lymphocytes in the early-stage and normal groups showed no significant difference (P = 0.151), while the late-stage (deeper dermis) groups decreased significantly compared with early-stage, late-stage (upper dermis) and normal groups (P < 0.001).

Conclusion: The densities of Langerhans cells, CD3 , CD4 and CD8 T cells, and the ratio of CD4 /CD8 T lymphocytes were different in different stages of LS, which supports the important role of cellular immunity in mechanisms of LS.
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http://dx.doi.org/10.1111/ajd.13525DOI Listing
May 2021

Mechanism in bradycardia induced by Trimethyltin chloride: Inhibition activity and expression of Na/K-ATPase and apoptosis in myocardia.

J Toxicol Sci 2020 ;45(9):549-558

Guangdong Province Hospital for Occupational Disease Prevention and Treatment, China.

Trimethyltin chloride (TMT) is a stabilizer by-product in the process of manufacturing plastic, which is a kind of very strong toxic substance, and has acute, cumulative and chronic toxicity. TMT may cause bradycardia in patients with occupational poisoning, the mechanism of which has not been reported. This study explored the mechanism of TMT resulting in bradycardia of C57BL/6 mice. TMT was administered to mice to measure heart rate, serum succinate dehydrogenase (SDH) level, and myocardial Na/K-ATPase activity and expression. The effects of TMT on myocardial apoptosis were observed by changing the expressions of caspase-3, Bax and Bcl-2 in myocardium. It was found that the heart rate and SDH activity in serum of mice gradually decreased with the increase of TMT dose compared with the control group. The activity and the expression of Na/K-ATPase in the heart tissue of mice exposed to TMT was measured and gradually decreased with the increase of dose and time. We measured the expression of Bcl-2, Bax, caspase-3 and cleaved caspase-3 in the heart tissues of TMT exposed mice and found that the expressions of Bax, caspase-3 and cleaved caspase-3 increased and the expressions of Bcl-2 decreased in the heart tissues of the TMT-exposed mice at different doses. With the extension of TMT exposure time, the expression of Bax and caspase-3 increased and the expression of Bcl-2 decreased in the heart tissues of TMT exposed mice. Our findings suggest the mechanisms of TMT resulting in bradycardia may be associated with the inhibited activity and decreased content of Na/K-ATPase, thus further leading to the changes of Bcl-2, Bax, caspase-3 and cleaved caspase-3 in the mice's ventricular tissues.
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http://dx.doi.org/10.2131/jts.45.549DOI Listing
October 2020

Choline supplementation improves the lipid metabolism of intrauterine-growth-restricted pigs.

Asian-Australas J Anim Sci 2018 May 22;31(5):686-695. Epub 2016 May 22.

College of Animal Science and Technology, Nanjing Agricultural University (NJAU), Nanjing 210095, China.

Objective: The objective of this study was to investigate the effects of dietary choline supplementation on hepatic lipid metabolism and gene expression in finishing pigs with intrauterine growth retardation (IUGR).

Methods: Using a 2×2 factorial design, eight normal birth weight (NBW) and eight IUGR weaned pigs were fed either a basal diet (NBW pigs fed a basal diet, NC; IUGR pigs fed a basal diet, IC) or a diet supplemented with two times more choline than the basal diet (NBW pigs fed a high-choline diet, NH; IUGR pigs fed a high-choline diet, IH) until 200 d of age.

Results: The results showed that the IUGR pigs had reduced body weight compared with the NBW pigs (p<0.05 from birth to d 120; p = 0.07 from d 120 to 200). Increased (p<0.05) free fatty acid (FFA) and triglyceride levels were observed in the IUGR pigs compared with the NBW pigs. Choline supplementation decreased (p<0.05) the levels of FFAs and triglycerides in the serum of the pigs. The activities of malate dehydrogenase and glucose 6-phosphate dehydrogenase were both increased (p<0.05) in the livers of the IUGR pigs. Choline supplementation decreased (p<0.05) malate dehydrogenase activity in the liver of the pigs. Gene expression of fatty acid synthase (FAS) was higher (p<0.05) in the IC group than in the other groups, and choline supplementation decreased (p<0.05) FAS and acetyl-CoA carboxylase α expression in the livers of the IUGR pigs. The expression of carnitine palmitoyl transferase 1A (CPT1A) was lower (p<0.05) in the IC group than in the other groups, and choline supplementation increased (p<0.05) the expression of CPT1A in the liver of the IUGR pigs and decreased (p<0.01) the expression of hormone-sensitive lipase in both types of pigs. The gene expression of phosphatidylethanolamine N-methyltransferase (PEMT) was higher (p<0.05) in the IC group than in the other groups, and choline supplementation significantly reduced (p<0.05) PEMT expression in the liver of the IUGR pigs.

Conclusion: In conclusion, the lipid metabolism was abnormal in IUGR pigs, but the IUGR pigs consuming twice the normal level of choline had improved circulating lipid parameters, which could be related to the decreased activity of nicotinamide adenine dinucleotide phosphate-generating enzymes or the altered expressions of lipid metabolism-related genes.
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http://dx.doi.org/10.5713/ajas.15.0810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5930279PMC
May 2018

The effect of hemoperfusion on patients with toxic encephalopathy induced by silkworm chrysalis ingestion.

Scott Med J 2016 Aug 26;61(3):149-154. Epub 2015 Nov 26.

Professor, Department of Emergency Medicine, The First Affiliated Hospital of Jilin University, China.

This study aims to determine therapeutic effect of hemoperfusion on patients with acute toxic encephalopathy induced by silkworm chrysalis ingestion. Three patients who developed toxic encephalopathy after chrysalis ingestion were analysed. Two patients lost their consciousness, while two patients had typical extrapyramidal tremor symptoms. Further neurological examination revealed various degrees of muscle strength impairment in these patients. All of them received treatments of omeprazole (40 mg/day), furosemide (one dose of 20 mg), vitamin C (2.0 g/day), calcium gluconate (2.0 g/day) and rehydration with glucose and sodium chloride (1500 ml/day). In addition, they received hemoperfusion treatment for 1.5 h. All patients recovered well after hemoperfusion. Two patients with loss of consciousness significantly recovered at 45 min and 65 min after hemoperfusion, respectively. All tremor symptoms were completely resolved in these patients at 30 min, 50 min, and 70 min following treatment, respectively. After the hemoperfusion treatment, encephalopathy symptoms of two patients had completely disappeared. All patients were followed up for one month and did not report any abnormalities. Our study indicates that hemoperfusion could be a useful and efficient treatment strategy for patients with acute encephalopathy after silkworm chrysalis ingestion. Larger clinical trials with longer follow-up are warranted to confirm the clinical benefit of hemoperfusion.
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http://dx.doi.org/10.1177/0036933015619291DOI Listing
August 2016

Microfluidic analysis of pressure drop and flow behavior in hypertensive micro vessels.

Biomed Microdevices 2015 ;17(3):9959

Department of Modern Mechanics, University of Science and Technology of China, Hefei, Anhui, China, 230027.

The retinal arterial network is the only source of the highly nutrient-consumptive retina, thus any insult on the arteries can impair the retinal oxygen and nutrient supply and affect its normal function. The aim of this work is to study the influences of vascular structure variation on the flow and pressure characteristics via microfluidic devices. Two sets of micro-channel were designed to mimic the stenosed microvessels and dichotomous branching structure in the retinal arteries. Three working fluids including red blood cell (RBC) suspension were employed to investigate the pressure drop in the stenosed channel. The flow behaviors of RBC suspensions inside the micro channels were observed using high speed camera system. Pressure drop of different working fluids and RBC velocity profiles in the stenosed channel were obtained. Moreover, hematocrit levels of RBC suspensions inside the bifurcated channels were analyzed from the sequential images of RBC flow. The results of the flow in the stenosed channel show that RBCs drift from the center of the channels, and RBC velocity is influenced not only by the inlet flow rate but also the interaction between RBCs. The measured pressure drops in the stenosed channel increase notably with the increase of fluid viscosity. Furthermore, the dimensionless pressure drop due to the stenosis decreases with Reynolds number. On the other hand, the results of flow through the bifurcated channels show that as the ratio of the daughter-branch width to the mother-channel width increases, the ratio of hematocrit in two connected branches (Ht/Hd) decreases, which is in favorable agreement with the available analysis results.
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http://dx.doi.org/10.1007/s10544-015-9959-4DOI Listing
December 2015
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