Publications by authors named "Jiaqi Li"

502 Publications

How to Achieve Sufficient Endogenous Insulin Suppression in Euglycemic Clamps Assessing the Pharmacokinetics and Pharmacodynamics of Long-Acting Insulin Preparations Employing Healthy Volunteers.

Front Pharmacol 2022 22;13:899798. Epub 2022 Jul 22.

Department of Endocrinology and Metabolism, Sichuan University West China Hospital, Chengdu, China.

The therapeutic effect of basal insulin analogs will be sustained at a rather low insulin level. When employing healthy volunteers to assess the pharmacokinetics (PK) and pharmacodynamics (PD) of long-acting insulin preparations by euglycemic clamp techniques, endogenous insulin cannot be ignored and sufficient endogenous insulin inhibition is crucial for the PD and/or PK assessment. This study aimed to explore a way to sufficiently inhibit endogenous insulin secretion. Healthy Chinese male and female volunteers were enrolled. After a subcutaneous injection of insulin glargine (IGlar) (LY2963016 or Lantus) (0.5 IU/kg), they underwent a manual euglycemic clamp for up to 24 h where the target blood glucose (BG) was set as 0.28 mmol/L below the individual's baseline. Blood samples were collected for analysis of PK/PD and C-peptide. The subjects fell into two groups according to the reduction extent of postdose C-peptide from baseline. After matching for the dosage proportion of Lantus, there were 52 subjects in group A (C-peptide reduction<50%) and 26 in group B (C-peptide reduction≥50%), respectively. No significant difference was detected in age, body mass index, the proportion of Latus treatment and female participants. A lower basal BG was observed in group B compared to group A (4.35 ± 0.26 vs 4.59 ± 0.22 mmol/L, < 0.05). The clamp studies were all conducted with high quality (where BG was consistently maintained around the target and exhibited a low variety). The binary logistic regression analysis indicated low basal BG as an independent factor for the success of sufficient endogenous insulin suppression. In conclusion, setting a lower sub-baseline target BG (e.g., 10% instead of 5% below baseline) might be an approach to help achieve sufficient endogenous insulin suppression in euglycemic clamps with higher basal BG levels (e.g., beyond 4.60 mmol/L).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2022.899798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354408PMC
July 2022

Construction of a cross-species cell landscape at single-cell level.

Nucleic Acids Res 2022 Aug 5. Epub 2022 Aug 5.

Women's Hospital, and Institute of Genetics, Zhejiang University School of Medicine, Hangzhou 310058, China.

Individual cells are basic units of life. Despite extensive efforts to characterize the cellular heterogeneity of different organisms, cross-species comparisons of landscape dynamics have not been achieved. Here, we applied single-cell RNA sequencing (scRNA-seq) to map organism-level cell landscapes at multiple life stages for mice, zebrafish and Drosophila. By integrating the comprehensive dataset of > 2.6 million single cells, we constructed a cross-species cell landscape and identified signatures and common pathways that changed throughout the life span. We identified structural inflammation and mitochondrial dysfunction as the most common hallmarks of organism aging, and found that pharmacological activation of mitochondrial metabolism alleviated aging phenotypes in mice. The cross-species cell landscape with other published datasets were stored in an integrated online portal-Cell Landscape. Our work provides a valuable resource for studying lineage development, maturation and aging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/nar/gkac633DOI Listing
August 2022

Visible and Near-Infrared Spectroscopy Combined With Bayes Classifier Based on Wavelength Model Optimization Applied to Wine Multibrand Identification.

Front Nutr 2022 18;9:796463. Epub 2022 Jul 18.

Department of Biological Engineering, Jinan University, Guangzhou, China.

The identification of high-quality wine brands can avoid adulteration and fraud and protect the rights and interests of producers and consumers. Since the main components of wine are roughly the same, the characteristic components that can distinguish wine brands are usually trace amounts and not unique. The conventional quantitative detection method for brand identification is complicated and difficult. The naive Bayes (NB) classifier is an algorithm based on probability distribution, which is simple and particularly suitable for multiclass discriminant analysis. However, the absorbance probability between spectral wavelengths is not necessarily strongly independent, which limits the application of Bayes method in spectral pattern recognition. This research proposed a Bayes classifier algorithm based on wavelength optimization. First, a large-scale wavelength screening for equidistant combination (EC) was performed, and then wavelength step-by-step phase-out (WSP) was carried out to reduce the correlation between wavelengths and improve the accuracy of Bayes discrimination. The proposed EC-WSP-Bayes method was applied to the 5-category discriminant analysis of wine brand identification based on visible and near-infrared (Vis-NIR) spectroscopy. Among them, four types of wine brands were collected from regular sales channels as identification brands. The fifth type of samples was composed of 21 other commercial brand wines and home-brewed wines from various sources, as the interference brand. The optimal EC-WSP-Bayes model was selected, the corresponding wavelength combination was 404, 600, 992, 2,070, 2,266, and 2,462 nm located in the visible light, shortwave NIR, and combination frequency regions. In modeling and independent validation, the total recognition accuracy rate (RAR ) reached 98.1 and 97.6%, respectively. The technology is quick and easy, which is of great significance to regulate the alcohol market. The proposed model of less-wavelength and high-efficiency ( = 6) can provide a valuable reference for small special instruments. The proposed integrated chemometric method can reduce the correlation between wavelengths, improve the recognition accuracy, and improve the applicability of the Bayesian method.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnut.2022.796463DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344138PMC
July 2022

Facile synthesis of mesoporous Ni Co S hollow spheres for high-performance supercapacitors and aqueous Ni/Co-Zn batteries.

RSC Adv 2022 Jul 14;12(32):20447-20453. Epub 2022 Jul 14.

College of Chemistry and Chemical Engineering, Anyang Normal University Anyang 455000 Henan China +86 372 2900040.

Porous micro/nanostructure electrode materials have always contributed to outstanding electrochemical energy storage performances. CoS is an ideal model electrode material with high theoretical specific capacity due to its intrinsic two crystallographic sites of cobalt ions. In order to improve the conductivity and specific capacitance of CoS, nickel ions were introduced to tune the electronic structure of CoS. The morphology design of the mesoporous hollow sphere structure guarantees cycle stability and ion diffusion. In this work, Ni Co S mesoporous hollow spheres were synthesized a facile partial ion-exchange of CoS mesoporous hollow spheres without using a template, boosting the capacitance to 1300 F g at the current density of 1 A g. Compared with the pure CoS and Ni-CoS-30%, Ni-CoS-60% exhibited the best supercapacitor performance, which was ascribed to the maximum Ni ion doping with morphology and structure retention, enhanced conductivity and stabilization of Co in the structure. Therefore, Ni/Co-Zn batteries were fabricated by using a Zn plate as the anode and Ni-CoS-60% as the cathode, which deliver a high energy density of 256.5 W h kg at the power density of 1.69 kW kg. Furthermore, the Ni/Co-Zn batteries exhibit a stable cycling after 3000 repeated cycles with capacitance retention of 69% at 4 A g. This encouranging result might provide a new perspective to optimize CoS-based electrodes with superior supercapacitor and Ni/Co-Zn battery performances.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2ra03022eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280778PMC
July 2022

A label-free dual-modal aptasensor for colorimetric and fluorescent detection of sulfadiazine.

J Mater Chem B 2022 Jul 27. Epub 2022 Jul 27.

College of Life Sciences, Chongqing Normal University, Chongqing, 401331, P. R. China.

Sulfadiazine (SDZ) residues in food products and the environment pose a serious threat to human health and ecological balance, thereby warranting the development of new methods for simple, rapid and accurate detection of these compounds. To this end, we developed a novel label-free dual-modal aptasensor for SDZ detection based on distance-dependent color change of gold nanoparticles (AuNPs) and fluorescence resonance energy transfer between AuNPs and rhodamine B (RhoB). In this aptasensor, the binding of the aptamer to SDZ causes unprotected AuNPs to aggregate in NaCl solution, which alters the color of the solution and restores the fluorescence of RhoB. Under optimal conditions, the aptasensor exhibited a linear colorimetric response in the SDZ concentration range of 50-1000 ng mL, and a linear fluorescence response in the SDZ concentration range of 4-256 ng mL. The limits of detection for colorimetric and fluorescent readings were 28 ng mL and 2 ng mL respectively. The recoveries of SDZ in the spiked real samples were 88.28-108.44% by colorimetry and 90.27-106.04% by fluorometry. Furthermore, the results of this aptasensor showed excellent correlation ( ≥ 0.9858) with HPLC findings. Taken together, these experimental results demonstrate that the proposed label-free dual-modal aptasensor can be employed to screen for SDZ contamination in food and environmental samples.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2tb01115hDOI Listing
July 2022

An AuNPs-Based Fluorescent Sensor with Truncated Aptamer for Detection of Sulfaquinoxaline in Water.

Biosensors (Basel) 2022 Jul 11;12(7). Epub 2022 Jul 11.

College of Life Science, Chongqing Normal University, Chongqing 401331, China.

Herein, we developed a novel truncation technique for aptamer sequences to fabricate highly sensitive aptasensors based on molecular docking and molecular dynamics simulations. The binding mechanism and energy composition of the aptamer/sulfaquinoxaline (SQX) complexes were investigated. We successfully obtained a new SQX-specific aptamer (SBA28-1: CCCTAGGGG) with high affinity (K = 27.36 nM) and high specificity determined using graphene oxide. This aptamer has a unique stem-loop structure that can bind to SQX. Then, we fabricated a fluorescence aptasensor based on SBA28-1, gold nanoparticles (AuNPs), and rhodamine B (RhoB) that presented a good linear range of 1.25-160 ng/mL and a limit of detection of 1.04 ng/mL. When used to analyze water samples, the aptasensor presented acceptable recovery rates of 93.1-100.1% and coefficients of variation (CVs) of 2.2-10.2%. In conclusion, the fluorescence aptasensor can accurately and sensitively detect SQX in water samples and has good application prospects.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/bios12070513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312917PMC
July 2022

Development of high-throughput UPLC-MS/MS using multiple reaction monitoring for quantitation of complex human milk oligosaccharides and application to large population survey of secretor status and Lewis blood group.

Food Chem 2022 Jul 20;397:133750. Epub 2022 Jul 20.

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Key Laboratory of Separation Science for Analytical Chemistry, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Human milk oligosaccharides (HMOs) have attracted increasing attention due to the emerging evidence of their positive roles for infant's health. A high-throughput method for absolute quantitation of the complex HMOs including multiple isomeric structures is important but very challenging, due to the highly divers nature and wide variation in content of HMOs from different individuals. Here we used UPLC-MS-MRM in the negative-ion mode for accurate quantitation of 23 complex HMOs in just 15 min. The selected oligosaccharides are in their native forms and include neutral and sialylated, fucosylated and non-fucosylated, linear and branched, and secretor and Lewis phenotype indicators. The well validated method with good sensitivity, recovery and reproducibility was then applied to a large population quantitative survey of 251 Chinese mothers from five different ethnic groups (Han, Zhuang, Hui, Mongolian and Tibetan) living in different geographical regions for their secretor's status and Lewis phenotypes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2022.133750DOI Listing
July 2022

Construction of the axolotl cell landscape using combinatorial hybridization sequencing at single-cell resolution.

Nat Commun 2022 Jul 22;13(1):4228. Epub 2022 Jul 22.

Center for Stem Cell and Regenerative Medicine, and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, China.

The Mexican axolotl (Ambystoma mexicanum) is a well-established tetrapod model for regeneration and developmental studies. Remarkably, neotenic axolotls may undergo metamorphosis, a process that triggers many dramatic changes in diverse organs, accompanied by gradually decline of their regeneration capacity and lifespan. However, the molecular regulation and cellular changes in neotenic and metamorphosed axolotls are still poorly investigated. Here, we develop a single-cell sequencing method based on combinatorial hybridization to generate a tissue-based transcriptomic landscape of the neotenic and metamorphosed axolotls. We perform gene expression profiling of over 1 million single cells across 19 tissues to construct the first adult axolotl cell landscape. Comparison of single-cell transcriptomes between the tissues of neotenic and metamorphosed axolotls reveal the heterogeneity of non-immune parenchymal cells in different tissues and established their regulatory network. Furthermore, we describe dynamic gene expression patterns during limb development in neotenic axolotls. This system-level single-cell analysis of molecular characteristics in neotenic and metamorphosed axolotls, serves as a resource to explore the molecular identity of the axolotl and facilitates better understanding of metamorphosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-022-31879-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307617PMC
July 2022

Use of the systemic inflammation response index (SIRI) as a novel prognostic marker for patients on peritoneal dialysis.

Ren Fail 2022 Dec;44(1):1227-1235

Department of General Practice, Zhujiang Hospital, Southern Medical University, Guangzhou, PR China.

Background: The systemic inflammatory response index (SIRI), a novel inflammation maker, has proven to be associated with prognostic outcomes in various diseases. However, few studies have been conducted assessing how SIRI may influence outcomes of patients on peritoneal dialysis (PD). Herein, we assessed the predictive value of SIRI on mortality all-cause mortality, including cardiovascular disease (CVD) in PD patients.

Methods: A total of 646 PD patients were enrolled in this study. PD patients received regular PD treatments at the Zhujiang Hospital from 1 January 2011 to 31 December 2018. SIRI values could be computed as follows: neutrophil count × monocyte count/lymphocyte count. Patients were divided into two groups according to the median level of SIRI. Cox regression analysis and Kaplan-Meier methods were applied to analyze the relationship between SIRI and mortality outcomes in PD patients.

Results: During the median 31-month follow-up period, 97 (15.0%) PD patients died from all-causes, and 47 (49.0%) died of CVD. Kaplan-Meier analyses revealed that a high SIRI corresponded to the high mortality of all-cause deaths, including CVD (both  < 0.001) in patients on PD. After adjusting for potential confounders, the higher SIRI level was significantly associated with an increased all-cause mortality (HR: 2.007, 95% CI: 1.304-3.088,  = 0.002) and cardiovascular mortality (HR: 2.847, 95% CI: 1.445-5.608,  = 0.002).

Conclusions: SIRI was a promising predictor of mortality in PD patients, with a higher SIRI corresponding to increased risk of mortality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/0886022X.2022.2100262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297720PMC
December 2022

Partially oxidized MXenes-derived C-TiO/TiC coupled with Fe-CN as a ternary Z-scheme heterojunction: Enhanced photothermal and photo-Fenton performance.

J Colloid Interface Sci 2022 Jun 24;626:639-652. Epub 2022 Jun 24.

Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education, School of Chemistry and Materials Science, Heilongjiang University, Harbin, China. Electronic address:

Photo-Fenton reaction combining the photocatalytic reaction and Fenton reaction showed excellent degradation performance. However, it highly demanded the catalysts to display outstanding activity in these two reactions. Herein, Fe-doped carbon nitride/MXenes-derived C-TiO/TiC (Fe-CN/TiC/C-TiO) was prepared via two steps: Fe-CN and TiC were assembled via face-to-face attachment, following by in-situ partial oxidation of TiC to C-TiO. DFT predicted a Z-scheme charge transfer routine via metallic TiC as bridge, which was verified by EPR and radical trapping experiments. Additionally, PDOS calculation revealed the charge density around the doped-Fe atoms was remarkably increased, leading to better HO activation, which was experimentally confirmed by high yield of OH. Moreover, Fe-CN/TiC/C-TiO possessed the high photothermal effect to accelerate the surface reaction. By taking advantage of these merits, the degradation rate of Fe-CN/TiC/C-TiO was at least 4.2 times higher than the reference catalysts. Our work provided an insight toward the g-CN/TiO-based photo-Fenton catalysts with high performance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2022.06.079DOI Listing
June 2022

Evaluating methylation of human ribosomal DNA at each CpG site reveals its utility for cancer detection using cell-free DNA.

Brief Bioinform 2022 07;23(4)

Ministry of Education Key Laboratory of Bioinformatics; Center for Synthetic and Systems Biology; Beijing National Research Center for Information Science and Technology; Department of Automation, Tsinghua University, Beijing 100084, China.

Ribosomal deoxyribonucleic acid (DNA) (rDNA) repeats are tandemly located on five acrocentric chromosomes with up to hundreds of copies in the human genome. DNA methylation, the most well-studied epigenetic mechanism, has been characterized for most genomic regions across various biological contexts. However, rDNA methylation patterns remain largely unexplored due to the repetitive structure. In this study, we designed a specific mapping strategy to investigate rDNA methylation patterns at each CpG site across various physiological and pathological processes. We found that CpG sites on rDNA could be categorized into two types. One is within or adjacent to transcribed regions; the other is distal to transcribed regions. The former shows highly variable methylation levels across samples, while the latter shows stable high methylation levels in normal tissues but severe hypomethylation in tumors. We further showed that rDNA methylation profiles in plasma cell-free DNA could be used as a biomarker for cancer detection. It shows good performances on public datasets, including colorectal cancer [area under the curve (AUC) = 0.85], lung cancer (AUC = 0.84), hepatocellular carcinoma (AUC = 0.91) and in-house generated hepatocellular carcinoma dataset (AUC = 0.96) even at low genome coverage (<1×). Taken together, these findings broaden our understanding of rDNA regulation and suggest the potential utility of rDNA methylation features as disease biomarkers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/bib/bbac278DOI Listing
July 2022

Free docosahexaenoic acid promotes ferroptotic cell death via lipoxygenase dependent and independent pathways in cancer cells.

Eur J Nutr 2022 Jul 9. Epub 2022 Jul 9.

Wuxi School of Medicine, Jiangnan University, Wuxi, 214122, Jiangsu Province, China.

Purpose: Ferroptosis is a form of regulated cell death that has the potential to be targeted as a cancer therapeutic strategy. But cancer cells have a wide range of sensitivities to ferroptosis, which limits its therapeutic potential. Accumulation of lipid peroxides determines the occurrence of ferroptosis. However, the type of lipid involved in peroxidation and the mechanism of lipid peroxide accumulation are less studied.

Methods: The effects of fatty acids (10 μM) with different carbon chain length and unsaturation on ferroptosis were evaluated by MTT and LDH release assay in cell lines derived from prostate cancer (PC3, 22RV1, DU145 and LNCaP), colorectal cancer (HT-29), cervical cancer (HeLa) and liver cancer (HepG2). Inhibitors of apoptosis, necroptosis, autophagy and ferroptosis were used to determine the type of cell death. Then the regulation of reactive oxygen species (ROS) and lipid peroxidation by docosahexaenoic acid (DHA) was measured by HPLC-MS and flow cytometry. The avtive form of DHA was determined by siRNA mediated gene silencing. The role of lipoxygenases was checked by inhibitors and gene silencing. Finally, the effect of DHA on ferroptosis-mediated tumor killing was verified in xenografts.

Results: The sensitivity of ferroptosis was positively correlated with the unsaturation of exogenously added fatty acid. DHA (22:6 n-3) sensitized cancer cells to ferroptosis-inducing reagents (FINs) at the highest level in vitro and in vivo. In this process, DHA increased ROS accumulation, lipid peroxidation and protein oxidation independent of its membrane receptor, GPR120. Inhibition of long chain fatty acid-CoA ligases and lysophosphatidylcholine acyltransferases didn't affect the role of DHA. DHA-involved ferroptosis can be induced in both arachidonate lipoxygenase 5 (ALOX5) negative and positive cells. Down regulation of ALOX5 inhibited ferroptosis, while overexpression of ALOX5 promoted ferroptosis.

Conclusion: DHA can effectively promote ferroptosis-mediated tumor killing by increasing intracellular lipid peroxidation. Both ALOX5 dependent and independent pathways are involved in DHA-FIN induced ferroptosis. And during this process, free DHA plays an important role.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00394-022-02940-wDOI Listing
July 2022

Research on water seepage detection technology of tunnel asphalt pavement based on deep learning and digital image processing.

Sci Rep 2022 Jul 7;12(1):11519. Epub 2022 Jul 7.

College of Civil Engineering, Chongqing Jiaotong University, Chongqing, 400074, China.

To improve the safety of road tunnel pavement, the research established road tunnel pavement water seepage recognition models based on deep learning technology, and a water seepage area extraction model based on image processing technology to finally achieve accurate detection of water seepage on tunnel pavements. First, the deep learning models EfficientNet water seepage recognition model and MobileNet water seepage recognition model were built, the models were trained with the self-collected pavement seepage data set, and the F1 score was introduced to evaluate the accuracy and comprehensive performance of the two models in predicting different categories of water seepage characteristics. Then three grayscale processing methods, the cvtColor function, mean method and maximum method, six global threshold segmentation methods, Otsu thresholding method, THRESH_BINARY, THRESH_BINARY_INV, THRESH_TRUNC, THRESH_TOZERO and THRESH_TOZERO_INV, three filtering methods, namely Gaussian filtering, median filtering and morphological open operation, as well as small connected domain removal, were used to reduce the noise of the images. Finally, the seepage area image calculation method was proposed based on the processed images to predict the actual pavement seepage area. The results show that the recognition accuracy of the EfficientNet water seepage recognition model is 99.85% and 97.53% in the training and validation sets respectively, which is 2.85% and 0.76% higher than the 97% and 96.77% of the MobileNet model. The average F1 score of the EfficientNet model is 95.22%, which is 5.05% higher than that of the MobileNet model, for the four types of seepage feature images: point seepage, line seepage, surface seepage and no seepage. The cvtColor function for grayscale processing, THRESH_BINARY for threshold segmentation and a combination of median filtering and morphological open operation for image noise reduction can effectively extract the seepage features. The area calculation is performed by the seepage area image calculation method, and the average error between the predicted value and the actual seepage area is 8.30%, which can better achieve the accurate extraction of the seepage area.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-022-15828-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262913PMC
July 2022

Interleukin-2 inducible T cell kinase (ITK) may participate in the anti-bacterial immune response of Nile tilapia via regulating T-cell activation.

Fish Shellfish Immunol 2022 Jun 29;127:419-426. Epub 2022 Jun 29.

State Key Laboratory of Estuarine and Coastal Research, School of Life Sciences, East China Normal University, Shanghai, 200241, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.

Interleukin-2 inducible T cell kinase (ITK) plays a predominant role in the T-cell receptor (TCR) signaling cascade to ensure valid T-cell activation and function. Nevertheless, whether it regulates T-cell response of early vertebrates remains unknown. Herein, we investigated the involvement of ITK in the lymphocyte-mediated adaptive immune response, and its regulation to T-cell activation in the Nile tilapia Oreochromis niloticus. Both sequence and structure of O. niloticus ITK (OnITK) were remarkably conserved with its homologues from other vertebrates, implying its potential conserved function. OnITK mRNA was extensively expressed in lymphoid-related tissues, and with the relative highest level in peripheral blood. Once Nile tilapia was infected by Edwardsiella piscicida, OnITK in splenic lymphocytes was significantly up-regulated on 7-day post infection at both transcription and translation levels, suggesting that OnITK might involve in the primary adaptive immune response of teleost. Furthermore, upon splenic lymphocytes were stimulated by T-cell specific mitogen PHA, OnITK mRNA and protein levels were dramatically elevated. More importantly, treatment of splenic lymphocytes with specific inhibitor significantly crippled OnITK expression, which in turn impaired the inducible expression of T-cell activation markers IFN-γ, IL-2 and CD122, indicating the critical roles of ITK in regulating T-cell activation of Nile tilapia. Taken together, our results suggest that ITK takes part in the lymphocyte-mediated adaptive immunity of tilapia, and is indispensable for T-cell activation of teleost. Our findings thus provide novel evidences for understanding the mechanism regulating T-cell immunity of early vertebrates, as well as the evolution of adaptive immune system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fsi.2022.06.044DOI Listing
June 2022

Impact of linkage disequilibrium heterogeneity along the genome on genomic prediction and heritability estimation.

Genet Sel Evol 2022 Jun 27;54(1):47. Epub 2022 Jun 27.

Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.

Background: Compared to medium-density single nucleotide polymorphism (SNP) data, high-density SNP data contain abundant genetic variants and provide more information for the genetic evaluation of livestock, but it has been shown that they do not confer any advantage for genomic prediction and heritability estimation. One possible reason is the uneven distribution of the linkage disequilibrium (LD) along the genome, i.e., LD heterogeneity among regions. The aim of this study was to effectively use genome-wide SNP data for genomic prediction and heritability estimation by using models that control LD heterogeneity among regions.

Methods: The LD-adjusted kinship (LDAK) and LD-stratified multicomponent (LDS) models were used to control LD heterogeneity among regions and were compared with the classical model that has no such control. Simulated and real traits of 2000 dairy cattle individuals with imputed high-density (770K) SNP data were used. Five types of phenotypes were simulated, which were controlled by very strongly, strongly, moderately, weakly and very weakly tagged causal variants, respectively. The performances of the models with high- and medium-density (50K) panels were compared to verify that the models that controlled LD heterogeneity among regions were more effective with high-density data.

Results: Compared to the medium-density panel, the use of the high-density panel did not improve and even decreased prediction accuracies and heritability estimates from the classical model for both simulated and real traits. Compared to the classical model, LDS effectively improved the accuracy of genomic predictions and unbiasedness of heritability estimates, regardless of the genetic architecture of the trait. LDAK applies only to traits that are mainly controlled by weakly tagged causal variants, but is still less effective than LDS for this type of trait. Compared with the classical model, LDS improved prediction accuracy by about 13% for simulated phenotypes and by 0.3 to ~ 10.7% for real traits with the high-density panel, and by ~ 1% for simulated phenotypes and by - 0.1 to ~ 6.9% for real traits with the medium-density panel.

Conclusions: Grouping SNPs based on regional LD to construct the LD-stratified multicomponent model can effectively eliminate the adverse effects of LD heterogeneity among regions, and greatly improve the efficiency of high-density SNP data for genomic prediction and heritability estimation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12711-022-00737-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235212PMC
June 2022

Effects of ginkgo leaf tablet on the pharmacokinetics of rosiglitazone in rats and its potential mechanism.

Pharm Biol 2022 Dec;60(1):1190-1197

School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, China.

Context: Ginkgo leaf tablet (GLT), a traditional Chinese herbal formula, is often combined with rosiglitazone (ROS) for type 2 diabetes mellitus treatment. However, the drug-drug interaction between GLT and ROS remains unknown.

Objective: To investigate the effects of GLT on the pharmacokinetics of ROS and its potential mechanism.

Materials And Methods: The pharmacokinetics of 10 mg/kg ROS with 100/200 mg/kg GLT as single-dose and 10-day multiple-dose administration were investigated in Sprague-Dawley rats. , the effects of GLT on the activity of CYP2C8 and CYP2C9 were determined in recombinant human yeast microsomes and rat liver microsomes with probe substrates.

Results: The of ROS increased from 2.14 ± 0.38 (control) to 2.79 ± 0.37 (100 mg/kg) and 3.26 ± 1.08 h (200 mg/kg) in the single-dose GLT administration. The AUC (139.69 ± 45.46 vs. 84.58 ± 39.87 vs. 66.60 ± 15.90 h·μg/mL) and (2.75 ± 0.70 vs. 1.99 ± 0.44 vs. 1.68 ± 0.35 h) decreased significantly after multiple-dose GLT treatment. The IC values of quercetin, kaempferol, and isorhamnetin, GLT main constituents, were 9.32, 7.67, and 11.90 μmol/L for CYP2C8, and 27.31, 7.57, and 4.59 μmol/L for CYP2C9. The multiple-dose GLT increased rat CYP2C8 activity by 44% and 88%, respectively.

Discussion And Conclusions: The metabolism of ROS is attenuated in the single dose of GLT by inhibiting CYP2C8 and CYP2C9 activity, and accelerated after the multiple-dose GLT treatment via inducing CYP2C8 activity in rats, indicating that the clinical dose of ROS should be adjusted when co-administrated with GLT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/13880209.2022.2087688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246016PMC
December 2022

Genomic Prediction Using LD-Based Haplotypes in Combined Pig Populations.

Front Genet 2022 9;13:843300. Epub 2022 Jun 9.

Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China.

The size of reference population is an important factor affecting genomic prediction. Thus, combining different populations in genomic prediction is an attractive way to improve prediction ability. However, combining multireference population roughly cannot increase the prediction accuracy as well as expected in pig. This may be due to different linkage disequilibrium (LD) pattern differences between population. In this study, we used the imputed whole-genome sequencing (WGS) data to construct LD-based haplotypes for genomic prediction in combined population to explore the impact of different single-nucleotide polymorphism (SNP) densities, variant representation (SNPs or haplotype alleles), and reference population size on the prediction accuracy for reproduction traits. Our results showed that genomic best linear unbiased prediction (GBLUP) using the WGS data can improve prediction accuracy in multi-population but not within-population. Not only the genomic prediction accuracy of the haplotype method using 80 K chip data in multi-population but also GBLUP for the multi-population (3.4-5.9%) was higher than that within-population (1.2-4.3%). More importantly, we have found that using the haplotype method based on the WGS data in multi-population has better genomic prediction performance, and our results showed that building haploblock in this scenario based on low LD threshold ( = 0.2-0.3) produced an optimal set of variables for reproduction traits in Yorkshire pig population. Our results suggested that whether the use of the haplotype method based on the chip data or GBLUP (individual SNP method) based on the WGS data were beneficial for genomic prediction in multi-population, while simultaneously combining the haplotype method and WGS data was a better strategy for multi-population genomic evaluation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2022.843300DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218795PMC
June 2022

New Sights Into Long Non-Coding RNA LINC01133 in Cancer.

Front Oncol 2022 7;12:908162. Epub 2022 Jun 7.

Key Laboratory of Preservation of Human Genetic Resources and DiseaseControl, Ministry of Education, Harbin Medical University, Harbin, China.

LINC01133 is a long intergenic non-coding RNA that regulates malignancy in several cancers, including those of the digestive, female reproductive, respiratory, and urinary system. LINC01133 is an extensively studied lncRNA that is highly conserved, and its relatively stable expression is essential for its robust biological function. Its expression is highly tissue-specific with a distinct subcellular localization. It functions as an oncogene or a tumor suppressor gene in different cancers multiple mechanisms, such as those that involve competing with endogenous RNA and binding to RNA-binding proteins or DNA. Moreover, the secretion and transportation of LINC01133 by extracellular vesicles in the tumor micro-environment is regulated by other cells in the tumor micro-environment. To date, two mechanisms, an increase in copy number and regulation of transcription elements, have been found to regulate LINC01133 expression. Clinically, LINC01133 is an ideal marker for cancer prognosis and a potential therapeutic target in cancer treatment regimes. In this review, we aimed to summarize the aforementioned information as well as posit future directions for LINC01133 research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2022.908162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209730PMC
June 2022

Augmented antibacterial mechanism of ZnO nanoparticles by labyrinthian-channel configuration of maize-stalk carbohydrate columns and sustainable strategy for water decontamination.

J Hazard Mater 2022 08 1;436:129258. Epub 2022 Jun 1.

Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming 650500, Yunnan, PR China.

Zinc oxide nanoparticles (ZnO NPs) as a broad-spectrum germicide in environmental remediation applications, is hindered by mild toxicity to organisms during water sterilization. To solve this dilemma, this work provided an eco-benign approach to utilize maize stalk with natural labyrinthine-channel configuration simultaneously acting as microbe trap and bactericide carrier to arouse bactericidal response of ZnO NPs. The preparation comprises in-situ growing ZnO NPs, accompanied by nanoscale delignification, leading to formed carbohydrate complex retaining the intricately porous structure of the stalk. Assembled by maize-stalk carbohydrate (MSC) composites with 9 short composites in serial, the elimination of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) reached up 6.7 and 4.8 orders of magnitude, respectively. Labyrinth-framework MSC provided favorable sites for fusiform flower-like ZnO NPs to strongly adsorb (adsorption energy 5.5-11.7 eV) phosphoryl-involved biomacromolecules of bacterial envelops, causing generation of stable Zn-P and Zn-O(H), then cell incompleteness, cellular redox imbalance and DNA damage. Breakthrough analysis exposed the MSC/ZnO-filter possessing remarkable features of antibacterial exhaustion rate (~ 1.06 g/L) and capacity (~ 9.6 × 10 CFU/g) which were comparable with Ag-based composites. As evaluated by the logistic and Gompertz models, the filters effectively sterilized 0.97-10 L of environmental waters to meet the requirements of drinking water.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2022.129258DOI Listing
August 2022

Tumor-microenvironment triggered signal-to-noise boosting nanoprobes for NIR-IIb fluorescence imaging guided tumor surgery and NIR-II photothermal therapy.

Biomaterials 2022 Jun 16;287:121636. Epub 2022 Jun 16.

Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, 350002, PR China; The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, PR China; Department of Translational Medicine, Xiamen Institute of Rare Earth Materials, Chinese Academy of Sciences, Xiamen, 361024, PR China. Electronic address:

High quantum yield quantum dots (QDs) with the emission in the sub-second near infrared window (NIR-IIb, 1500-1700 nm) can afford higher resolution, a deeper penetration depth and zero auto-fluorescence for bio-imaging. However, low tumor accumulation, the rapid renal clearance and potential toxicity impeding their biomedical applications. Here, we report a tumor microenvironment responsive hollowed virus-bionic MnO nanoshell with IR1061 loading in the cavity and QDs ([email protected]) anchoring on the surface for precise NIR-IIb fluorescence imaging guided tumor surgery and efficient NIR-II photothermal therapy. This QDs based nanoprobe could efficiently adhere on tumor cells to realize efficient tumor tissue accumulation. NIR-IIb fluorescence of tumor margin could be successfully delineating after extracellular weak acid triggered MnO biodegradation for IR1061 release with remarkable NIR-IIb signal-to-noise boosting. Then, it could facilitate complete dissection of various tumor models with the assistance of NIR-IIb fluorescence imaging. Moreover, the fascinating efficacy for micro-metastasis eradication via NIR-II photothermal effects can be achieved under NIR-IIb fluorescence imaging guidance. Specifically, in combination with negligible system toxicity, our nanoprobes showed great potential as a versatile NIR-IIb fluorescent imaging platform for precise tumor surgery and tumor therapy guidance for future clinical translation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biomaterials.2022.121636DOI Listing
June 2022

Correction to: Clinical and radiographic outcomes of hybrid graft in patients with Modic changes undergoing transforaminal lumbar interbody fusion.

J Orthop Surg Res 2022 Jun 16;17(1):318. Epub 2022 Jun 16.

Department of Spinal Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, 050051, People's Republic of China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13018-022-03198-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205014PMC
June 2022

Microscale Confinement and Wetting Contrast Enable Enhanced and Tunable Condensation.

ACS Nano 2022 Jun 13. Epub 2022 Jun 13.

Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.

Dropwise condensation represents the upper limit of thermal transport efficiency for liquid-to-vapor phase transition. A century of research has focused on promoting dropwise condensation by attempting to overcome limitations associated with thermal resistance and poor surface-modifier durability. Here, we show that condensation in a microscale gap formed by surfaces having a wetting contrast can overcome these limitations. Spontaneous out-of-plane condensate transfer between the contrasting parallel surfaces decouples the nanoscale nucleation behavior, droplet growth dynamics, and shedding processes to enable minimization of thermal resistance and elimination of surface modification. Experiments on pure steam combined with theoretical analysis and numerical simulation confirm the breaking of intrinsic limits to classical condensation and demonstrate a gap-dependent heat-transfer coefficient with up to 240% enhancement compared to dropwise condensation. Our study presents a promising mechanism and technology for compact energy and water applications where high, tunable, gravity-independent, and durable phase-change heat transfer is required.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.2c02669DOI Listing
June 2022

Insights into the mechanism of carbon chain growth on zeolite-based Fischer-Tropsch Co/Y catalysts.

Phys Chem Chem Phys 2022 Jun 22;24(24):14751-14762. Epub 2022 Jun 22.

Key Laboratory for Green Chemical Technology of Ministry of Education, R&D Center for PetrochemicalTechnology, Tianjin University, Tianjin 300072, China.

In a zeolite-based Fischer-Tropsch bifunctional catalyst, zeolites, as the support of the active metal, can interact with the metal cluster to affect the electronic properties and structural effect of the catalyst, thus affecting the Fischer-Tropsch synthesis reaction. In this work, the Fischer-Tropsch synthesis process using a Co catalyst supported by Y-zeolite was simulated by the DFT method from the microscopic point of view. The reaction network was designed to investigate the reaction mechanism in terms of four parts consisting of H-assisted CO dissociation, C hydrogenation, CH-CH coupling, and C-C growth. It was found that the introduction of Y-zeolite enhanced the adsorption capacity of the catalyst for most species. Moreover, the catalytic mechanism of the Co/Y catalyst was clarified, and we found that the introduction of the Y-zeolite mainly reduced the reaction energy barriers of the CH-CH coupling and C-C carbon chain growth process, which also explained the high proportion of long carbon chain hydrocarbons in the Fischer-Tropsch synthesis products after Y-zeolite was introduced.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2cp01287aDOI Listing
June 2022

Synthesis of Tetrahydrocarbazol-4-ones via Rh(III)-Catalyzed C-H Activation/Annulation of Arylhydrazines with Iodonium Ylides.

J Org Chem 2022 06 8;87(12):8142-8150. Epub 2022 Jun 8.

College of Chemistry and Materials Science, Inner Mongolia Minzu University, Tongliao 028000, China.

The rhodium(III)-catalyzed C-H activation followed by intramolecular annulation reactions between arylhydrazines and iodonium ylides under suitable conditions has been described. Tetrahydrocarbazol-4-ones are readily achieved with moderate to excellent yields. The synthetic protocol features a wide range of substrates with high functional group tolerance. The gram-scale reaction and derivatization of the product demonstrate the synthetic practicality and utilization of this method.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.joc.2c00852DOI Listing
June 2022

Effect of different intervention methods on postoperative local recurrence of endometrial cancer: A systematic review and meta-analysis.

Asian J Surg 2022 May 31. Epub 2022 May 31.

Department of General Surgery, Dalian University Affiliated Xinhua Hospital, Dalian, 116000, China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.asjsur.2022.05.056DOI Listing
May 2022

Comprehensive Analysis of Spinal Muscular Atrophy: SMN1 Copy Number, Intragenic Mutation, and 2 + 0 Carrier Analysis by Third-Generation Sequencing.

J Mol Diagn 2022 May 31. Epub 2022 May 31.

International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China; Institute of Birth Defects and Rare Diseases, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. Electronic address:

Population-wide carrier screening for spinal muscular atrophy (SMA) is recommended by the American College of Medical Genetics and Genomics. However, the methods used currently mainly focus on SMN1 copy number and fail to identify carriers with pathogenic intragenic mutations and silent (2 + 0) carriers. We developed a method termed comprehensive analysis of SMA (CASMA) based on long-range PCR and third-generation sequencing of full-length and downstream regions of SMN1/2. The sensitivity and specificity of CASMA to detect SMA carriers with one copy of SMN1 were 100% (n = 101) and 99.2% (n = 236), respectively. CASMA confirmed three SMN1 intragenic mutations and pinpointed an inframe mutation c.661_666del to SMN2, which was misreported to SMN1 by allele-specific long-range nested PCR plus Sanger sequencing. CASMA also correctly predicted 8 of 16 samples (50%) with SMN1 duplication alleles. CASMA was expected to increase the detection rate of SMA carriers from 91% to 98% and decrease the residual risk ratio from 1:415 to 1:1868 after negative results of two SMN1 copies in the Chinese population. CASMA presents a comprehensive approach for identifying SMN1 and SMN2 copy number, intragenic mutations, and potential silent carriers that significantly reduces the residual risk ratio in SMA carrier screening and has great clinical utility.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jmoldx.2022.05.001DOI Listing
May 2022

Relationship between different body composition and bone mineral density in Qinhuangdao city.

Rev Assoc Med Bras (1992) 2022 Apr;68(4):445-449

The First Hospital of Qinhuangdao, Department of Functional Inspection - Qinhuangdao, China.

Objective: This study aimed to explore the correlation between different body components and bone mineral density in healthy adults.

Methods: A total of 306 non-manual subjects, 161 males and 145 females, were selected from the physical examination center of our hospital from June to September 2019. They were divided into control group, overweight group, and obese group according to body mass index. The muscle mass and fat mass, body fat content, trunk fat mass, upper limb and thigh fat mass, bone density of femoral neck and lumbar vertebra, and bone mineral salt content of the whole body were measured by dual-energy X-ray absorptiometry.

Results: Body mass index, systolic blood pressure, diastolic blood pressure, femoral neck bone mineral density, bone mineral salt content, fat mass, muscle mass, upper limb fat mass, thigh fat mass, and trunk fat mass in the overweight group and obese group were all higher than those in the control group (P<0.05). The fat mass, muscle mass, upper limb fat mass, and trunk fat mass were positively correlated with the femoral neck bone mineral density, total lumbar vertebra bone mineral density, and bone mineral salt content (P<0.05). In addition, thigh fat mass was positively correlated with femoral neck bone mineral density and total lumbar spine bone mineral density, whereas body fat content was negatively correlated with bone mineral salt content.

Conclusion: Body composition was related to bone mineral density and bone mineral salt content, and the correlation between different body composition indexes, and bone mineral density, and bone mineral salt content was different.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1590/1806-9282.20210669DOI Listing
April 2022

SARS-CoV-2 and Emerging Variants: Unmasking Structure, Function, Infection, and Immune Escape Mechanisms.

Front Cell Infect Microbiol 2022 12;12:869832. Epub 2022 May 12.

Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.

As of April 1, 2022, over 468 million COVID-19 cases and over 6 million deaths have been confirmed globally. Unlike the common coronavirus, SARS-CoV-2 has highly contagious and attracted a high level of concern worldwide. Through the analysis of SARS-CoV-2 structural, non-structural, and accessory proteins, we can gain a deeper understanding of structure-function relationships, viral infection mechanisms, and viable strategies for antiviral therapy. Angiotensin-converting enzyme 2 (ACE2) is the first widely acknowledged SARS-CoV-2 receptor, but researches have shown that there are additional co-receptors that can facilitate the entry of SARS-CoV-2 to infect humans. We have performed an in-depth review of published papers, searching for co-receptors or other auxiliary membrane proteins that enhance viral infection, and analyzing pertinent pathogenic mechanisms. The genome, and especially the spike gene, undergoes mutations at an abnormally high frequency during virus replication and/or when it is transmitted from one individual to another. We summarized the main mutant strains currently circulating global, and elaborated the structural feature for increased infectivity and immune evasion of variants. Meanwhile, the principal purpose of the review is to update information on the COVID-19 outbreak. Many countries have novel findings on the early stage of the epidemic, and accruing evidence has rewritten the timeline of the outbreak, triggering new thinking about the origin and spread of COVID-19. It is anticipated that this can provide further insights for future research and global epidemic prevention and control.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcimb.2022.869832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134119PMC
May 2022

Multi-Decker Emissive Supramolecular Architectures Based on Shape-Complementary Ligands Pair.

Small 2022 Jul 31;18(26):e2202167. Epub 2022 May 31.

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, Jilin, 130012, China.

Dye aggregates have attracted a great deal of attention due to their widespread applications in organic light-emitting devices, light-harvesting systems, etc. However, the strategies to precisely control chromophores with specific spatial arrangements still remain a great challenge. In this work, a series of double- and triple-decker supramolecular complexes are successfully constructed by coordination-driven self-assembly of carefully designed shape-complementary ligands, one claw-like tetraphenylethylene (TPE)-based host ligand and three tetratopic or ditopic guest ligands. The spatial configurations of these assemblies (one double-decker and three "S-shaped" or "X-shaped" triple-decker structures) depend on the angles of these TPE-derived ligands. Notably, the three triple-decker structures are geometric isomers. Furthermore, photophysical studies show that these complexes exhibit different ratios of radiative (k ) and non-radiative (k ) rate constant due to the different spatial arrangements of TPE moieties. This study provides not only a unique strategy for the construction of multi-stacks with specific spatial arrangement, but also a promising platform for investigating the aggregation behavior of fluorescent chromophores.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202202167DOI Listing
July 2022

Characterization of lncRNA Profiles of Plasma-Derived Exosomes From Type 1 Diabetes Mellitus.

Front Endocrinol (Lausanne) 2022 12;13:822221. Epub 2022 May 12.

National Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology (Central South University), Ministry of Education, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, China.

Backgrounds: Exosomes contain several types of transcripts, including long non-coding RNAs (lncRNAs), and have been shown to exert important effects in human diseases. However, the roles of exosomal lncRNAs in type 1 diabetes mellitus (T1DM) have not been well investigated. In the present study, we characterized the plasma-derived exosomal lncRNAs expression profiles of T1DM and predict their potential function in the pathogenesis of T1DM.

Material And Methods: Exosomal lncRNA expression profiles were detected by Illumina Hiseq platform (T1DM subjects N=10; age-, sex- matched Control subjects N=10). Six exosomal lncRNAs were selected to validate their expression level by using quantitative real-time PCR (qRT-PCR) (T1DM subjects N=30; age-, sex- matched Control subjects N=30). Bioinformatics analysis approaches were carried out to explore the potential biological function of differentially expressed lncRNAs.

Results: A total of 162 differentially expressed exosomal lncRNAs were identified in T1DM patients compared with control subjects, among which 77 up-regulated and 85 down-regulated. The expression level of the selected six lncRNAs didn't show significant difference in the following qRT-PCR analysis. Gene Ontology analysis enriched terms such as activation of phospholipase D activity, neuronal cell body membrane, and calcium sensitive guanylate cyclase activator activity for cis-acting genes of lncRNAs, and metal ion binding for trans-acting genes. The most enriched Kyoto Encyclopedia of Genes and Genomes pathways for the lncRNAs were associated with oxidative phosphorylation and Parkinson's disease for cis-acting genes, and pathways in cancer as well as focal adhesion for trans-acting genes.

Conclusions: This study characterized the lncRNA profiles of plasma-derived exosomes from T1DM for the first time and these results highlighted the potential role of exosomal lncRNAs in T1DM pathogenesis. A better understanding of exosomal lncRNA profiling will provide novel insights into its molecular mechanisms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fendo.2022.822221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135040PMC
May 2022
-->