Publications by authors named "Jiaqi Guo"

62 Publications

Diverse axial chiral assemblies of J-aggregates in plexcitonic nanoparticles.

Nanoscale 2021 Sep 16. Epub 2021 Sep 16.

State Key Laboratory of Information Photonics and Optical Communications, School of Science, Beijing University of Posts and Telecommunications, Beijing 100876, China.

Plexcitonic hybrids, consisting of metal nanoparticles and J-aggregates, are effective nanostructures to achieve a strong coupling regime. The chirality of the exciton in the strong coupled plexcitons provides more potential for the design of advanced optoelectronic devices. Here, we experimentally measured the circular dichroism (CD) spectra of plexcitonic hybrids, and researched the diverse chirality of J-aggregates assembled on the surface of the achiral Au nanorods. We found that the chirality of J-aggregates is not only related to the quantity of dye molecules in the plexcitonic, but also to the distribution in different positions of the nanorods, by analyzing the composition of the CD spectra with a quasistatic theory. The J-aggregates assembled on both ends and both sides of the nanorods had opposite chirality. The interaction between the longitudinal localized surface plasmon resonance (LLSPR) of the nanorods and J-aggregates achieved the strong coupling regime, and Rabi splitting of about 198.3 meV was observed. The research into the chirality of the plexcitons provided more detail on the chiral J-aggregates assembly on the nanoparticles, and give a perspective on the development of the strong coupling interactions and the design of optoelectronic systems.
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http://dx.doi.org/10.1039/d1nr02634hDOI Listing
September 2021

N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings.

Vet Microbiol 2021 Oct 25;261:109215. Epub 2021 Aug 25.

Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District, Chengdu City, Sichuan Province, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District, Chengdu City, Sichuan Province, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Wenjiang District, Chengdu City, Sichuan Province, 611130, China. Electronic address:

Duck Tembusu virus (DTMUV) is an emerging mosquito-borne flavivirus that has caused acute egg-drop syndrome in egg-laying ducks. DTMUV nonstructural protein 1 (NS1) contains three potential predicted N-linked glycosylation sites at residues 130, 175 and 207. In this study, we found that mutations at these sites affect the molecular weight of recombinant NS1, as assessed by western blot assays; however, the mutations do not affect their subcellular localization in the cytoplasm, as assessed by colocalization assays. Four recombinant viruses substituting the asparagine (N) residues at N130, N175, N207 or N130/N175/N207 of NS1 with alanine (A) residues were generated using rDTMUV-i, an infectious cDNA clone of the DTMUV CQW1 strain. Deglycosylation assays of the mutant virus NS1 were performed using endoglycosidases Endo H or PNGase F treatment in both mammalian and avian cells. The NS1-WT, NS1-N130A, NS1-N175A and NS1-N207A showed a shift in migration to 37 kDa after digestion with both endoglycosidases, which further confirmed that N130, N175 and N207 were the glycosylation sites of DTMUV NS1. Compared to the parental rDTMUV, the single mutants impaired viral multiplication in vitro, while the nonglycosylated virus rDTMUV-NS1-N130A/N175A/N207A showed a 5-fold to 178-fold decrease in viral titers and smaller plaque sizes. Notably, all mutant viruses were still highly virulent to duck embryos, but the embryos inoculated with rDTMUV-NS1-N130A/N175A/N207A started to die on the fourth day, which exhibited a prolonged time to death compared to that of rDTMUV. Moreover, rDTMUV-NS1-N130A/N175A/N207A was attenuated in vivo, showing no mortality and producing significantly lower viral titers in heart, spleen, kidney, brain and thymus as well as 2-fold to 3-fold lower viremia at 3 and 5 days post infection. Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo.
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http://dx.doi.org/10.1016/j.vetmic.2021.109215DOI Listing
October 2021

Replication/Assembly Defective Avian Flavivirus With Internal Deletions in the Capsid Can Be Used as an Approach for Living Attenuated Vaccine.

Front Immunol 2021 4;12:694959. Epub 2021 Aug 4.

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

Avian Tembusu virus (TMUV) is a novel flavivirus causing severe egg drop and fatal encephalitis in avian in Asia. In the present study, we screened the structural and functional requirements of TMUV capsid protein (CP) for viral morphogenesis using reverse genetics methods in combination with replicon packaging assays. TMUV-CP showed dramatic functional and structural flexibility, and even though 44 residues were removed from the N-terminus, it was still capable of packaging replicon RNA; in addition, 33 residues were deleted from the C-terminus (containing nearly the entire α4-helix), and infectious particles were still produced, although α4-α4' is supposedly vital for CP dimerization and nucleocapsid formation. We further analyzed two mutants (ΔC20-43 and ΔC64-96 viruses) with relatively large deletions that still replicated well in BHK-21 cells. Our data indicate that internal deletions within CP impaired viral replication or assembly, resulting in attenuated virus proliferation in cells and attenuated virulence in duck embryos, and these deletion mutations are quite stable in cell culture. An assay indicated that both ΔC20-43 virus and ΔC64-96 virus were highly attenuated in ducklings but still immunogenic. Single-dose immunization with ΔC20-43 virus or ΔC64-96 virus could protect ducklings from a lethal challenge with good antigen clearance. Together, our data shed light on replication/assembly defective TMUV with internal deletions in CP and provide an effective approach to attenuate viral virulence in live vaccines without changing the antigen composition.
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http://dx.doi.org/10.3389/fimmu.2021.694959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371329PMC
August 2021

Replication/Assembly Defective Avian Flavivirus With Internal Deletions in the Capsid Can Be Used as an Approach for Living Attenuated Vaccine.

Front Immunol 2021 4;12:694959. Epub 2021 Aug 4.

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

Avian Tembusu virus (TMUV) is a novel flavivirus causing severe egg drop and fatal encephalitis in avian in Asia. In the present study, we screened the structural and functional requirements of TMUV capsid protein (CP) for viral morphogenesis using reverse genetics methods in combination with replicon packaging assays. TMUV-CP showed dramatic functional and structural flexibility, and even though 44 residues were removed from the N-terminus, it was still capable of packaging replicon RNA; in addition, 33 residues were deleted from the C-terminus (containing nearly the entire α4-helix), and infectious particles were still produced, although α4-α4' is supposedly vital for CP dimerization and nucleocapsid formation. We further analyzed two mutants (ΔC20-43 and ΔC64-96 viruses) with relatively large deletions that still replicated well in BHK-21 cells. Our data indicate that internal deletions within CP impaired viral replication or assembly, resulting in attenuated virus proliferation in cells and attenuated virulence in duck embryos, and these deletion mutations are quite stable in cell culture. An assay indicated that both ΔC20-43 virus and ΔC64-96 virus were highly attenuated in ducklings but still immunogenic. Single-dose immunization with ΔC20-43 virus or ΔC64-96 virus could protect ducklings from a lethal challenge with good antigen clearance. Together, our data shed light on replication/assembly defective TMUV with internal deletions in CP and provide an effective approach to attenuate viral virulence in live vaccines without changing the antigen composition.
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http://dx.doi.org/10.3389/fimmu.2021.694959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371329PMC
August 2021

Surface-enhanced Raman scattering of flexible cotton fiber-Ag for rapid adsorption and detection of malachite green in fish.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Dec 13;263:120174. Epub 2021 Jul 13.

School of Petrochemical Engineering, Liaoning Petrochemical University, Fushun, Liaoning 113001, PR China. Electronic address:

The residual of malachite green (MG) in fish is one of the major food safety concerns for consumers. It is important to develop simple and instant analytical methods to identify MG residues in fish. We fabricated flexible cotton surface-enhanced Raman scattering substrate, which offers good flexibility, uniformity and excellent adsorption capability. The UV-vis DRS spectra, transmission electron microscopy and scanning electron elemental mapping images shown that the Ag NPs were closely packed on the surface of cotton fiber. The adsorption feature of cotton fiber could adsorb MG from solution and surface of fish. The Quick Easy Cheap Effective Rugged and Safe (QuEChERS) sample preparation method was used to adsorb MG in fish for SERS sensing. The limit of detection of MG in fish using this developed method was as low as 0.05 ppm. The QuEChERS-SERS analysis method exhibits the capability for multiplex detecting mixture of MG and Dimetridazole at different ratios (5 ppm, 1/400 and 1/4000) from fish. The results indicated that the cotton fiber-Ag composite was suitable employed as SERS substrate for simple and instant detecting trace contaminants in food.
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http://dx.doi.org/10.1016/j.saa.2021.120174DOI Listing
December 2021

Fabrication and Application of SERS-Active Cellulose Fibers Regenerated from Waste Resource.

Polymers (Basel) 2021 Jun 29;13(13). Epub 2021 Jun 29.

School of Petrochemical Engineering, Liaoning Petrochemical University, Fushun 113001, China.

The flexible SERS substrate were prepared base on regenerated cellulose fibers, in which the Au nanoparticles were controllably assembled on fiber through electrostatic interaction. The cellulose fiber was regenerated from waste paper through the dry-jet wet spinning method, an eco-friendly and convenient approach by using ionic liquid. The Au NPs could be controllably distributed on the surface of fiber by adjusting the conditions during the process of assembling. Finite-difference time-domain theoretical simulations verified the intense local electromagnetic fields of plasmonic composites. The flexible SERS fibers show excellent SERS sensitivity and adsorption capability. A typical Raman probe molecule, 4-Mercaptobenzoicacid (4-MBA), was used to verify the SERS cellulose fibers, the sensitivity could achieve to 10 M. The flexible SERS fibers were successfully used for identifying dimetridazole (DMZ) from aqueous solution. Furthermore, the flexible SERS fibers were used for detecting DMZ from the surface of fish by simply swabbing process. It is clear that the fabricated plasmonic composite can be applied for the identifying toxins and chemicals.
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http://dx.doi.org/10.3390/polym13132142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272151PMC
June 2021

Lung parenchyma parameters measure of rats from pulmonary window computed tomography images based on ResU-Net model for medical respiratory researches.

Math Biosci Eng 2021 05;18(4):4193-4211

College of Medicine and Biological Information Engineering, Northeastern University, Shenyang 110169, China.

Our paper proposes a method to measure lung parenchyma parameters from pulmonary window computed tomography images based on ResU-Net model including the CT value, the density, the lung volume, and the surface area of the lungs of healthy rats, to help promote the quantitative analysis of lung parenchyma parameters of rats in medical respiratory researches. Through the analysis of the lung parenchyma parameters of the control group and the treatment group, the law of change among the lung parenchyma parameters is given in our paper. After comparing and analyzing the lung parenchyma parameter CT value and the density of the two groups, it is discovered that the lung parenchyma parameter CT value and the density significantly increase in the treatment group which is after continuously inhaling the nebulization of contrast agents. The change of the lung volume with the surface area in both two groups conforms to the law of lung changes during breathing. The relationship between the lung volume and the CT value or the density is analyzed and it is concluded that the lung volume is negatively correlated with the CT value or the density.
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http://dx.doi.org/10.3934/mbe.2021210DOI Listing
May 2021

Amelioration of Beta Interferon Inhibition by NS4B Contributes to Attenuating Tembusu Virus Virulence in Ducks.

Front Immunol 2021 17;12:671471. Epub 2021 May 17.

Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, China.

Our previous studies reported that duck Tembusu virus nonstructural protein 2A (NS2A) is a major inhibitor of the IFNβ signaling pathway through competitively binding to STING with TBK1, leading to a reduction in TBK1 phosphorylation. Duck TMUV NS2B3 could cleave and bind STING to subvert the IFNβ signaling pathway. Here, we found that overexpression of duck TMUV NS4B could compete with TBK1 in binding to STING, reducing TBK1 phosphorylation and inhibiting the IFNβ signaling pathway by using the Dual-Glo Luciferase Assay System and the NanoBiT protein-protein interaction (PPI) assay. We further identified the E2, M3, G4, W5, K10 and D34 residues in NS4B that were important for its interaction with STING and its inhibition of IFNβ induction, which were subsequently introduced into a duck TMUV replicon and an infectious cDNA clone. We found that the NS4B M3A mutant enhanced RNA replication and exhibited significantly higher titer levels than WT at 48-72 hpi but significantly decreased mortality (80%) in duck embryos compared to WT (100%); the NS4B G4A and R36A mutants slightly reduced RNA replication but exhibited the same titer levels as WT. However, the NS4B R36A mutant did not attenuate the virulence in duck embryos, whereas the G4A mutant significantly decreased the mortality (70%) of duck embryos. In addition, the NS4B W5A mutant did not affect viral replication, whereas the D34A mutant slightly reduced RNA replication, and both mutants exhibited significantly lower titer levels than the WT and significantly decreased mortality (90% and 70%, respectively) in duck embryos. Hence, our findings provide new insight into the development of attenuated flaviviruses by targeting the disabling viral strategies used to evade the innate defense mechanisms.
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http://dx.doi.org/10.3389/fimmu.2021.671471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165282PMC
May 2021

Enhancing the Radical Scavenging Activity and UV Resistance of Lignin Nanoparticles via Surface Mannich Amination toward a Biobased Antioxidant.

Biomacromolecules 2021 06 2;22(6):2693-2701. Epub 2021 Jun 2.

The Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Jiangnan University, Wuxi 214122, China.

In recent years, lignin specific activities, such as antioxidation and antibacterial and anti-ultraviolet performance, have drawn more and more attention. Nevertheless, the insufficient radical scavenging (antioxidation) activity has become one of the main drawbacks that limits its high-value application. In this study, lignin nanoparticles (LNPs) were prepared via a facile acid treatment strategy. Subsequently, surface amination of LNPs (a-LNPs) was carried out through the Mannich reaction. Specifically, the antioxidant behavior of LNPs and modified LNPs was evaluated by DPPH/DMPO radical scavenging and HeLa cell reactive oxygen species (ROS) scavenging tests, which demonstrated that the antioxidation activity of a-LNPs was more evident than that of both LNPs and butylated hydroxytoluene (BHT) commercial antioxidant. The mechanism of the radical scavenging ability of aminated LNPs was elucidated and proved to be related to the bond dissociation enthalpy of Ar-O···H, determined by the electron-donating effect of the substituted groups in the -position. Meanwhile, the morphologies, solubilities, and UV-absorbing and antibacterial behavior of LNPs and a-LNPs were also studied, and the results showed that a-LNP sample exhibited higher UV resistance performance than LNPs. We expected that the modified LNPs with high antioxidation activity can serve as a safe and lower-cost biobased antioxidant.
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http://dx.doi.org/10.1021/acs.biomac.1c00387DOI Listing
June 2021

Dynamic Continuum of Nanoscale Peptide Assemblies Facilitates Endocytosis and Endosomal Escape.

Nano Lett 2021 05 3;21(9):4078-4085. Epub 2021 May 3.

Department of Chemistry, Brandeis University, 415 South Street, Waltham, Massachusetts 02453, United States.

Alkaline phosphatase (ALP) enables intracellular targeting by peptide assemblies, but how the ALP substrates enter cells remains elusive. Here we show that nanoscale phosphopeptide assemblies cluster ALP to enable caveolae-mediated endocytosis (CME) and endosomal escape. Specifically, fluorescent phosphopeptides undergo enzyme-catalyzed self-assembly to form nanofibers. Live cell imaging unveils that phosphopeptides nanoparticles, coincubated with HEK293 cells overexpressing red fluorescent protein-tagged tissue-nonspecific ALP (TNAP-RFP), cluster TNAP-RFP in lipid rafts to enable CME. Further dephosphorylation of the phosphopeptides produces peptidic nanofibers for endosomal escape. Inhibiting TNAP, cleaving the membrane anchored TNAP, or disrupting lipid rafts abolishes the endocytosis. Decreasing the transformation to nanofibers prevents the endosomal escape. As the first study establishing a dynamic continuum of nanoscale assemblies for cellular uptake, this work illustrates an effective design for enzyme-responsive supramolecular therapeutics and provides mechanism insights for understanding the dynamics of cellular uptake of proteins or exogenous peptide aggregates.
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http://dx.doi.org/10.1021/acs.nanolett.1c01029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180093PMC
May 2021

Fabrication of plasmonic cotton gauze-Ag composite as versatile SERS substrate for detection of pesticides residue.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Aug 31;257:119766. Epub 2021 Mar 31.

School of Petrochemical Engineering, Liaoning Petrochemical University, Fushun, Liaoning 113001, PR China. Electronic address:

Plasmonic cotton gauze-Ag composite were fabricated through a simple, instant and cost-effective way, in which the Ag NPs were immobilized on the surface of cotton gauze through in-situ growth process. The in-situ growth of Ag NPs was started from electroless-immobilized Ag seeds on the surface of cotton fiber, which could form numerous hot spots for SERS compared with current method. The cotton gauze-Ag composite was employed as versatile substrate in surface-enhanced Raman scattering (SERS) spectroscopy. The plasmonic cotton gauze-Ag exhibited excellent uniformity, temporal stability and enhanced effect for SERS measurement. The detection limit of P-aminothiopheno (PATP) was 10 M. Furthermore, the plasmonic cotton gauze-Ag composite presented excellent flexibility and adsorption capability, which enable to adsorb and detect pesticide residue from irregular surface of cucumber directly by simple swabbing process, the detection limit could achieve 0.1 ppm. The cotton gauze-Ag composite also shown excellent selectivity is SERS sensing. The fabrication method could be simply extended to other cellulose compound, such as absorbent cotton, paper and even for natural fibers. This study proposed a new method for fabricating the cost-effective, eco-friendly and flexible SERS substrates.
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http://dx.doi.org/10.1016/j.saa.2021.119766DOI Listing
August 2021

Temporal physical activity patterns are associated with obesity in U.S. adults.

Prev Med 2021 07 30;148:106538. Epub 2021 Mar 30.

Department of Nutrition Science, Purdue University, West Lafayette, IN 47907, USA. Electronic address:

Few attempts have been made to incorporate multiple aspects of physical activity (PA) to classify patterns linked with health. Temporal PA patterns integrating time and activity counts were created to determine their association with health status. Accelerometry data from the National Health and Nutrition Examination Survey 2003-2006 was used to pattern PA counts and time of activity from 1999 adults with one weekday of activity. Dynamic time warping and kernel k-means clustering partitioned 4 participant clusters representing temporal PA patterns. Multivariate regression models determined associations between clusters and health status indicators and obesity, type 2 diabetes, and metabolic syndrome. Cluster 1 with a temporal PA pattern of the lowest activity counts reaching 4.8e cph from 6:00-23:00 was associated with higher body mass index (BMI) (β = 2.5 ± 0.6 kg/m, 95% CI: 1.0, 4.1), higher waist circumference (WC) (β = 6.4 ± 1.3 cm, 95% CI: 2.8, 10.0), and higher odds of obesity (OR: 2.4; 95% CI: 1.3, 4.4) compared with Cluster 3 with activity counts reaching 9.6e-1.2e cph between 16:00-21:00. Cluster 1 was also associated with higher BMI (β = 1.5 ± 0.5 kg/m, 95% CI: 0.1, 2.8) and WC (β = 3.6 ± 1.3 cm, 95% CI: 0.1, 7.0) compared to Cluster 4 with activity counts reaching 9.6e cph between 8:00-11:00. A Temporal PA pattern with the lowest PA counts had significantly higher mean BMI and WC compared to temporal PA patterns of higher activity counts performed early (8:00-11:00) or late (16:00-21:00) throughout the day. Temporal PA patterns appear to meaningfully link to health status.
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http://dx.doi.org/10.1016/j.ypmed.2021.106538DOI Listing
July 2021

Impacts of degree of substitution of quaternary cellulose on the strength improvement of fiber networks.

Int J Biol Macromol 2021 Jun 23;181:41-44. Epub 2021 Mar 23.

Department of Chemical Engineering, University of New Brunswick, Fredericton, NB E3B 5A3, Canada.

The degree of substitution (DS) of cellulose derivative is significantly associated with its properties. In this paper, a series of quaternary cellulose (QC) samples with different DS (ranging from 0.16 to 0.51) were synthesized with assistance of microwave and their relationship with strength improvement of fiber networks was investigated systematically. QCs were characterized by elemental analysis, FT-IR, H NMR, and TGA, etc. The results showed that the cationic quaternary ammonium salt group was successfully grafted onto the backbones of cellulose chains and the thermal stability was associated inversely with the DS of QCs. However, the results of strength test for the fiber networks from secondary fiber of old corrugated containers showed that the tensile and burst strength was enhanced by addition of QCs, and their performance was positively correlated their DS. The best result achieved in this investigation was in the case of QC with DS of 0.51, with increments of tensile and burst strength 6.17% and 11.68%, respectively, at a dosage of 1.0 wt% based on oven-dry pulp. This investigation highlights the importance of DS of QC to its application in strength improvement for fiber networks.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.03.121DOI Listing
June 2021

High Expression of LINC01268 is Positively Associated with Hepatocellular Carcinoma Progression via Regulating MAP3K7.

Onco Targets Ther 2021 8;14:1753-1769. Epub 2021 Mar 8.

Department of Gastroenterology, First Affiliated Hospital of China Medical University, Shenyang, 110001, People's Republic of China.

Objective: As one of the most common neoplastic diseases, hepatocellular carcinoma (HCC) has a high morbidity and mortality, which seriously threatens human health and places a heavy burden on society and medical care. At present, effective early diagnosis, prognosis and treatment of HCC are limited. Altered gene expression patterns of lncRNA are associated with the occurrence, development and prognosis of various malignancies, including HCC. The aim of this study was to investigate the correlation between the expression of LINC01268 and HCC, and to elucidate the potential underlying molecular mechanism.

Methods: Expression level and localization of LINC01268 in human liver cancer cells and HCC tissues were investigated using RT-qPCR and fluorescent in situ hybridization (FISH), respectively. Correlation of expression levels of LINC01268 and MAP3K7 with differentiation and poor overall patient survival of HCC were analyzed using in house collected and publicly available HCC tissue data. RT-qPCR and Western blot were applied to inspect the effects of depletion and overexpression of LINC01268 on MAP3K7 expression. HCC cell proliferation and apoptosis were also investigated by simultaneous overexpression of LINC01268 and knockdown of MAP3K7, in order to delineate that MAP3K7 is a downstream effector of LINC01268.

Results: In this study, we identified that LINC01268 was highly expressed in HCC cell lines and tissues. High LINC01268 expression level was associated with lower HCC nodule number, moderate/poor differentiation and poor overall survival. Knockdown of LINC01268 inhibited the proliferation of HCC cells, which was enhanced by overexpression of LINC01268. Co-expression analysis implied an interaction between LINC01268 and MAP3K7. Similar to LINC01268, MAP3K7 was highly expressed in HCC cells, and positively correlated with moderate/poor differentiation as well as poor prognosis. Knockdown of LINC01268 in HCC cell lines led to reduction of MAP3K7 at both mRNA and protein levels. Phenotypic effects due to LINC01268 overexpression in HCC cells were reversed by knockdown of MAP3K7.

Conclusion: Taken together, the abnormal high expression of LINC01268 is associated with HCC progression via regulating MAP3K7, suggesting LINC01268 as a novel marker for HCC prognosis and potentially a new therapeutic target.
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http://dx.doi.org/10.2147/OTT.S295814DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954037PMC
March 2021

Performance of zinc oxide quantum dots coated paper and application of fluorescent anti-counterfeiting.

Appl Opt 2021 Mar;60(8):2304-2313

Fluorescent anti-counterfeiting is one of the most widely used anti-counterfeiting technologies at present. The demand to develop new anti-counterfeiting materials and technology is more and more urgent. Zinc oxide quantum dots (ZnO QDs) have superior fluorescent properties under ultraviolet light, making them a suitable replacement for traditional phosphors for anti-counterfeiting printing, which is environmentally friendly and meets the needs of sustainable development. In this paper, water-soluble ZnO QDs with an average particle size of 5.64 nm were prepared. Paper coated by ZnO QDs was obtained after ultrasonic treatment, which could emit bright yellow fluorescence when excited by ultraviolet light. As the concentration of ultrasonic solution is increased, the loading amount of ZnO QDs on the coated paper increased gradually, reaching the maximum when the concentration is increased to 1, which then does not change with an increase in concentration. The fluorescent intensity of the coated paper was consistent with the changing trend of the loading amount. The coated paper has excellent optical stability, is easy to recycle, and provides simple identification of authenticity by ultraviolet light and anti-copy functionality. Their application in packaging and printing is of great significance to the development of complex, concealed and non-repeatable anti-counterfeiting technology.
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http://dx.doi.org/10.1364/AO.416896DOI Listing
March 2021

Electrical switching of high-performance bioinspired nanocellulose nanocomposites.

Nat Commun 2021 02 26;12(1):1312. Epub 2021 Feb 26.

Institute for Macromolecular Chemistry, University of Freiburg, Freiburg, Germany.

Nature fascinates with living organisms showing mechanically adaptive behavior. In contrast to gels or elastomers, it is profoundly challenging to switch mechanical properties in stiff bioinspired nanocomposites as they contain high fractions of immobile reinforcements. Here, we introduce facile electrical switching to the field of bioinspired nanocomposites, and show how the mechanical properties adapt to low direct current (DC). This is realized for renewable cellulose nanofibrils/polymer nanopapers with tailor-made interactions by deposition of thin single-walled carbon nanotube electrode layers for Joule heating. Application of DC at specific voltages translates into significant electrothermal softening via dynamization and breakage of the thermo-reversible supramolecular bonds. The altered mechanical properties are reversibly switchable in power on/power off cycles. Furthermore, we showcase electricity-adaptive patterns and reconfiguration of deformation patterns using electrode patterning techniques. The simple and generic approach opens avenues for bioinspired nanocomposites for facile application in adaptive damping and structural materials, and soft robotics.
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http://dx.doi.org/10.1038/s41467-021-21599-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910463PMC
February 2021

Enzymatic Noncovalent Synthesis for Mitochondrial Genetic Engineering of Cancer Cells.

Cell Rep Phys Sci 2020 Dec 9;1(12). Epub 2020 Dec 9.

Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454, USA.

Since mitochondria contribute to tumorigenesis and drug resistance in cancer, mitochondrial genetic engineering promises a new direction for cancer therapy. Here, we report the use of the perimitochondrial enzymatic noncovalent synthesis (ENS) of peptides for delivering genes selectively into the mitochondria of cancer cells for mitochondrial genetic engineering. Specifically, the micelles of peptides bind to the voltage-dependent anion channel (VDAC) on mitochondria for the proteolysis by enterokinase (ENTK), generating perimitochondrial nanofibers in cancer cells. This process, facilitating selective delivery of nucleic acid or gene vectors into mitochondria of cancer cells, enables the mitochondrial transgene expression of CRISPR/Cas9, FUNDC1, p53, and fluorescent proteins. Mechanistic investigation indicates that the interaction of the peptide assemblies with the VDAC and mitochondrial membrane potential are necessary for mitochondria targeting. This local enzymatic control of intermolecular noncovalent interactions enables selective mitochondrial genetic engineering, thus providing a strategy for targeting cancer cells.
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http://dx.doi.org/10.1016/j.xcrp.2020.100270DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7839975PMC
December 2020

Plexcitonic Optical Chirality: Strong Exciton-Plasmon Coupling in Chiral J-Aggregate-Metal Nanoparticle Complexes.

ACS Nano 2021 Feb 28;15(2):2292-2300. Epub 2020 Dec 28.

State Key Laboratory of Information Photonics and Optical Communications, School of Science, Beijing University of Posts and Telecommunications, 10 Xitucheng Road, Beijing 100876, China.

Understanding the unique characteristics of plexcitons, hybridized states resulting from the strong coupling between plasmons and excitons, is vital for both fundamental studies and practical applications in nano-optics. However, the research of plexcitons from the perspective of chiral optics has been rarely reported. Here, we experimentally investigate the optical chirality of plexcitonic systems consisting of composite metal nanoparticles and chiral J-aggregates in the strong coupling regime. Mode splitting and anticrossing behavior are observed in both the circular dichroism (CD) and extinction spectra of the hybrid nanosystems. A large mode splitting (at zero detuning) of up to 136 meV/214 meV in CD/extinction measurements confirms that the systems attain the strong coupling regime. This phenomenon indicates that the formation of plexcitons modifies not only the extinction but also the optical chirality of the hybrid systems. We develop a quasistatic theory to elucidate the chiral optical responses of hybrid systems. Furthermore, we propose and justify a criterion of strong plasmon-exciton interaction: the mode splitting in the CD spectra (at zero detuning) is larger than half of that in the extinction spectra. Our findings give a chiral perspective on the study of strong plasmon-exciton coupling and have potential applications in the chiral optical field.
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http://dx.doi.org/10.1021/acsnano.0c08274DOI Listing
February 2021

Fine-tuning of polariton energies in a tailored plasmon cavity and J-aggregates hybrid system.

Nanoscale 2020 Nov;12(45):23069-23076

State Key Laboratory of Information Photonics and Optical Communications, School of Science, Beijing University of Posts and Telecommunications, Beijing 100876, China.

Strong coupling systems enable coherent energy exchange between a light field and material electrons in nanoscale space. Active manipulation of this phenomenon by external stimuli is crucial for the design of advanced optoelectronic devices. Two neglected points severely hinder the improvement of tuning accuracy: irreversible variation in cavity morphology and lack of control over the dielectric environment which may change during the coupling process. Here we present a chemical fine-tuning of the strong plasmon-exciton coupling process in tailored [email protected] nanocavities. The silver shell thickness was carefully controlled to tune the plasmon resonance wavelength with an accuracy of ∼8 nm and facilitate hot spots at the edges to boost the plasmon-exciton coupling strength. Hybrid polariton states were further regulated across the zero-detuning point with a spectral accuracy of less than 1 nm via tuning the solvent refractive index, and a Rabi splitting as large as 194 meV was observed at room temperature. The fine-tuning of strong plasmon-exciton coupling by an adjacent dielectric environment provides a novel route to manipulate excitons in molecules and possesses great potential for chemical or biological sensing.
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http://dx.doi.org/10.1039/d0nr06376bDOI Listing
November 2020

Temporal Dietary Patterns Are Associated with Obesity in US Adults.

J Nutr 2020 12;150(12):3259-3268

Department of Nutrition Science, Purdue University, West Lafayette, IN, USA.

Background: The integration of time with dietary patterns throughout a day, or temporal dietary patterns (TDPs), have been linked with dietary quality but relations to health are unknown.

Objective: The association between TDPs and selected health status indicators and obesity, type 2 diabetes (T2D), and metabolic syndrome (MetS) was determined.

Methods: The first-day 24-h dietary recall from 1627 nonpregnant US adult participants aged 20-65 y from the NHANES 2003-2006 was used to determine timing, amount of energy intake, and sequence of eating occasions (EOs). Modified dynamic time warping (MDTW) and kernel k-means algorithm clustered participants into 4 groups representing distinct TDPs. Multivariate regression models determined associations between TDPs and health status, controlling for potential confounders, and adjusting for the survey design and multiple comparisons (P <0.05/6).

Results: A cluster representing a TDP with evenly spaced, energy balanced EOs reaching ≤1200 kcal between 06:00 to 10:00, 12:00 to 15:00, and 18:00 to 22:00, had statistically significant and clinically meaningful lower mean BMI (P <0.0001), waist circumference (WC) (P <0.0001), and 75% lower odds of obesity compared with 3 other clusters representing patterns with much higher peaks of energy: 1000-2400 kcal between 15:00 and 18:00 (OR: 5.3; 95% CI: 2.8, 10.1), 800-2400 kcal between 11:00 and 15:00 (OR: 4.4; 95% CI: 2.5, 7.9), and 1000-2600 kcal between 18:00 and 23:00 (OR: 6.7; 95% CI: 3.9, 11.6).

Conclusions: Individuals with a TDP characterized by evenly spaced, energy balanced EOs had significantly lower mean BMI, WC, and odds of obesity compared with the other patterns with higher energy intake peaks at different times throughout the day, providing evidence that incorporating time with other aspects of a dietary pattern may be important to health status.
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http://dx.doi.org/10.1093/jn/nxaa287DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726125PMC
December 2020

Biomaterials based on noncovalent interactions of small molecules.

EXCLI J 2020 5;19:1124-1140. Epub 2020 Aug 5.

Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02453, USA.

Unlike conventional materials that covalent bonds connecting atoms as the major force to hold the materials together, supramolecular biomaterials rely on noncovalent intermolecular interactions to assemble. The reversibility and biocompatibility of supramolecular biomaterials render them with diverse range of functions and lead to rapid development in the past two decades. This review focuses on the noncovalent and enzymatic control of supramolecular biomaterials, with the introduction to various triggering mechanism to initiate self-assembly. Representative applications of supramolecular biomaterials are highlighted in four categories: tissue engineering, cancer therapy, drug delivery, and molecular imaging. By introducing various applications, we intend to show enzymatic control and noncovalent interactions as a powerful tool for achieving spatiotemporal control of biomaterials both and for biomedicine.
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http://dx.doi.org/10.17179/excli2020-2656DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573174PMC
August 2020

Self-Assembled Bioinspired Nanocomposites.

Acc Chem Res 2020 11 29;53(11):2622-2635. Epub 2020 Sep 29.

A3BMS Lab-Active, Adaptive and Autonomous Bioinspired Materials, Institute for Macromolecular Chemistry, University of Freiburg, Stefan-Meier-Straße 31, 79104 Freiburg, Germany.

Bioinspired materials engineering impacts the design of advanced functional materials across many domains of sciences from wetting behavior to optical and mechanical materials. In all cases, the advances in understanding how biology uses hierarchical design to create failure and defect-tolerant materials with emergent properties lays the groundwork for engaging into these topics. Biological mechanical materials are particularly inspiring for their unique combinations of stiffness, strength, and toughness together with lightweightness, as assembled and grown in water from a limited set of building blocks at room temperature. Wood, nacre, crustacean cuticles, and spider silk serve as some examples, where the correct arrangement of constituents and balanced molecular energy dissipation mechanisms allows overcoming the shortcomings of the individual components and leads to synergistic materials performance beyond additive behavior. They constitute a paradigm for future structural materials engineering-in the formation process, the use of sustainable building blocks and energy-efficient pathways, as well as in the property profiles-that will in the long term allow for new classes of high-performance and lightweight structural materials needed to promote energy efficiency in mobile technologies.This Account summarizes our efforts of the past decade with respect to designing self-assembling bioinspired materials aiming for both mechanical high-performance structures and new types of multifunctional property profiles. The Account is set out to first give a definition of bioinspired nanocomposite materials and self-assembly therein, followed by an in-depth discussion on the understanding of mechanical performance and rational design to increase the mechanical performance. We place a particular emphasis on materials formed at high fractions of reinforcements and with tailor-made functional polymers using self-assembly to create highly ordered structures and elucidate in detail how the soft polymer phase needs to be designed in terms of thermomechanical properties and sacrificial supramolecular bonds. We focus on nanoscale reinforcements such as nanoclay and nanocellulose that lead to high contents of internal interfaces and intercalated polymer layers that experience nanoconfinement. Both aspects add fundamental challenges for macromolecular design of soft phases using precision polymer synthesis. We build upon those design criteria and further develop the concepts of adaptive bioinspired nanocomposites, whose properties are switchable from the outside using molecularly defined triggers with light. In a last section, we discuss how new types of functional properties, in particular flexible and transparent gas barrier materials or fire barrier materials, can be reached on the basis of the bioinspired nanocomposite design strategies. Additionally, we show new types of self-assembled photonic materials that can even be evolved into self-assembling lasers, hence moving the concept of mechanical nanocomposite design to other functionalities.The comparative discussion of different bioinspired nanocomposite architectures with nematic, fibrillar, and cholesteric structures, as based on different reinforcing nanoparticles, aims for a unified understanding of the design principles and shall aid researchers in the field in the more elaborate design of future bioinspired nanocomposite materials based on molecular control principles. We conclude by addressing challenges, in particular also the need for a transfer from fundamental molecular materials science into scalable engineering materials of technological and societal relevance.
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http://dx.doi.org/10.1021/acs.accounts.0c00448DOI Listing
November 2020

Enzymatic Noncovalent Synthesis.

Chem Rev 2020 09 19;120(18):9994-10078. Epub 2020 Aug 19.

Department of Chemistry, Brandeis University, 415 South Street, Waltham, Massachusetts 02454, United States.

Enzymatic reactions and noncovalent (i.e., supramolecular) interactions are two fundamental nongenetic attributes of life. Enzymatic noncovalent synthesis (ENS) refers to a process where enzymatic reactions control intermolecular noncovalent interactions for spatial organization of higher-order molecular assemblies that exhibit emergent properties and functions. Like enzymatic covalent synthesis (ECS), in which an enzyme catalyzes the formation of covalent bonds to generate individual molecules, ENS is a unifying theme for understanding the functions, morphologies, and locations of molecular ensembles in cellular environments. This review intends to provide a summary of the works of ENS within the past decade and emphasize ENS for functions. After comparing ECS and ENS, we describe a few representative examples where nature uses ENS, as a rule of life, to create the ensembles of biomacromolecules for emergent properties/functions in a myriad of cellular processes. Then, we focus on ENS of man-made (synthetic) molecules in cell-free conditions, classified by the types of enzymes. After that, we introduce the exploration of ENS of man-made molecules in the context of cells by discussing intercellular, peri/intracellular, and subcellular ENS for cell morphogenesis, molecular imaging, cancer therapy, and other applications. Finally, we provide a perspective on the promises of ENS for developing molecular assemblies/processes for functions. This review aims to be an updated introduction for researchers who are interested in exploring noncovalent synthesis for developing molecular science and technologies to address societal needs.
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http://dx.doi.org/10.1021/acs.chemrev.0c00306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7530130PMC
September 2020

Design of uniform permanent magnet electronic optical system for 220 GHz sheet electron beam traveling wave tube.

Sci Rep 2020 Aug 13;10(1):13680. Epub 2020 Aug 13.

Key Laboratory of High Power Microwave Sources and Technologies, Aerospace Information Research Institute, Chinese Academy of Science, Beijing, 100190, China.

The sheet electron beam (SEB), for which is the low current density and large current, is highly attractive in the region of millimeter wave and terahertz vacuum electronic devices (VEDs). A uniform permanent magnet (UPM) electronic optical system (EOS) driven by a SEB for 220 GHz traveling wave tube (TWT) is designed in present work, in which the voltage and current for SEB is 17 kV and 0.3 A, respectively. For obtaining the stable high transmission rate EOS, the characteristics of SEB in UPM EOS are studied, including the emittance, orbital angle, and beam trajectories, which are discussed through the CST simulation. The results show that the emittances in the x-direction are varied from 0.003 to 0.016 mm rad and in y-direction are various from 1 × 10 to 3 × 10 mm rad, respectively, keeping below than 2.5 × 10 mm rad during transmission, which guarantees the stability of SEB in y-direction. For the design of complete EOS, the normal rectangular collector is used, in which the SEB is uniformed scattering.
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http://dx.doi.org/10.1038/s41598-020-70016-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7427102PMC
August 2020

Long and Ultrastable All-Inorganic Single-Crystal CsPbBrMicrowires: One-Step Solution In-Plane Self-Assembly at Low Temperature and Application for High-Performance Photodetectors.

J Phys Chem Lett 2020 Sep 19;11(17):7224-7231. Epub 2020 Aug 19.

Guangdong Engineering Research Center of Optoelectronic Functional Materials and Devices, Institute of Semiconductors, South China Normal University, Guangzhou 510631, People's Republic of China.

As ideal building blocks for optoelectronic devices, one-dimensional (1D) single-crystal perovskite microwires (MWs) have received widespread attention due to their unique physical and chemical properties. Herein, a one-step solution in-plane self-assembly method is proposed to directly grow millimeter-long CsPbBr MWs with superior crystal quality at atmospheric environment. This method effectively avoids the use of toxic antisolvents. Furthermore, a MW-based photodetector is successfully fabricated, showing high photoresponsivity (20 A/W) and fast response (less than 0.3 ms). The stability of the photodetector is also confirmed by aging MW in air for 60 days, which shows a negligible change of photocurrent from 1.29 to 1.25 nA (-3 V) under the same experimental conditions. This work provides a low-cost and fast synthesis method for the preparation of single-crystal perovskite MWs and demonstrates their potential application for high-performance and stable photoelectronic device.
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http://dx.doi.org/10.1021/acs.jpclett.0c01920DOI Listing
September 2020

High prevalence and risk factors of multiple antibiotic resistance in patients who fail first-line Helicobacter pylori therapy in southern China: a municipality-wide, multicentre, prospective cohort study.

J Antimicrob Chemother 2020 11;75(11):3391-3394

Department of Medicine, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China.

Background: We aimed to study the prevalence of secondary antibiotic resistance of Helicobacter pylori in southern China and its risk factors, particularly geographical and socio-economic factors.

Methods: This was a municipality-wide, multicentre, prospective cohort study involving five major hospitals. Patients aged ≥18 years who failed first-line bismuth-based quadruple anti-H. pylori therapy between September 2016 and February 2018 were recruited. Participants underwent upper gastrointestinal endoscopy with biopsy from the antrum and body for H. pylori culture and antimicrobial susceptibility testing for six antibiotics (clarithromycin, levofloxacin, metronidazole, amoxicillin, tetracycline and furazolidone). Patients with failure of H. pylori culture were excluded. Participants completed a questionnaire profiling 22 potential risk factors of H. pylori infection and antibiotic resistance, including medical, social, household and birthplace factors.

Results: A total of 1113 patients failed first-line therapy, with successful H. pylori culture in 791 (71.1%) [male = 433 (54.7%); median age = 43 years]. Secondary resistance rates of dual antibiotics (clarithromycin + metronidazole and levofloxacin + metronidazole) and triple antibiotics (clarithromycin + levofloxacin + metronidazole) were 34.0%, 38.7% and 17.8%, respectively. Risk factors for clarithromycin + metronidazole resistance were history of ≥2 courses of H. pylori therapies [adjusted OR (aOR) = 1.71; 95% CI = 1.17-2.54], ≥3 household members (aOR = 2.00; 95% CI = 1.07-3.90) and family history of gastric cancer (aOR = 1.85; 95% CI = 1.18-2.85). Risk factors for levofloxacin + metronidazole resistance were age ≥40 years (aOR = 1.94; 95% CI = 1.37-2.75), lower gross domestic product per capita (aOR = 0.29; 95% CI = 0.10-0.80) and higher number of doctors/10 000 population (aOR = 1.59; 95% CI = 1.07-2.39). A higher human development index was of borderline significance (aOR = 2.79; 95% CI = 0.97-8.70).

Conclusions: The rates of secondary resistance of H. pylori to multiple antibiotics were high in southern China. Certain population-level risk factors were associated with levofloxacin + metronidazole resistance.
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http://dx.doi.org/10.1093/jac/dkaa315DOI Listing
November 2020

The ratio of hydrogelator to precursor controls the enzymatic hydrogelation of a branched peptide.

Soft Matter 2020 Nov;16(44):10101-10105

Department of Chemistry, Brandeis University, 415 South Street, Waltham, MA 02453, USA.

Here, we report an apparently counterintuitive observation, in which a lower volume fraction of a branched peptide forms a stronger hydrogel after an enterokinase (ENTK) cleaves off the branch from the peptide. By varying the ratios of the branched peptide and ENTK and analysing the ratio of hydrogelator to precursor (H/P) in the enzymatic proteolysis reaction, our study shows that the H/P ratio controls the critical strain of the hydrogel formed, through enzymatic cleavage of the branch from the peptide. This work demonstrates that emergent properties (e.g., hydrogelation) of peptide assemblies, resulting from enzymatic noncovalent synthesis (ENS), are context-dependent, while also providing insights for developing dynamic soft materials via ENS.
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http://dx.doi.org/10.1039/d0sm00867bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677216PMC
November 2020

An RGD modified water-soluble fluorophore probe for in vivo NIR-II imaging of thrombosis.

Biomater Sci 2020 Aug 10;8(16):4438-4446. Epub 2020 Jul 10.

Department of Cardiology, Zhongda Hospital, Medical School of Southeast University, 87 Dingjiaqiao, Nanjing, Jiangsu 210009, China.

Venous thrombosis leads to severe symptoms and death through pulmonary embolism. There is a great need for high sensitivity imaging methods to identify acute patients who would benefit from thrombolysis. We designed a novel, organic near-infrared second-window (NIR-II) probe, which targets the glycoprotein IIb/IIIa receptor (GPIIb/IIIa) on activated platelets. The probe's structure was characterized by MALDI-TOF-MS, TEM, UV-visible absorption and NIR-II fluorescent spectroscopy. The probe's specificity for activated platelets was investigated in vitro and in vivo. Thrombosis in mice was induced by administration of FeCl in the external jugular vein and imaged by using a NIR-II imager. The donor-acceptor-donor fluorescent core TTQ was prepared from donor and acceptor units. TTQ-PEG-NH was synthesized by sequential modification of PEGylated TTQ, followed by c(RGD) condensation. Signal strength was continuously monitored for 24 h following TTQ-PEG-c(RGD) or non-specific fluorescent dye injection. The contralateral external jugular vein, sham surgery and a competitive inhibition experiment served as controls. TTQ-PEG-c(RGD) presented high NIR-II intensity, good stability and excellent affinity for activated platelets. The NIR-II fluorescence signal of TTQ-PEG-c(RGD) injected mice significantly increased at the thrombus site and peaked at 4 h, whereas there was no significant change in the control mice, and the competitive inhibition of the RGD antagonist suppressed the enhancement of the NIR-II fluorescence signal. Comparison between fresh and old thrombi confirmed that TTQ-PEG-c(RGD) could be used to distinguish a fresh thrombus from an old thrombus. TTQ-PEG-c(RGD) can specifically target thrombosis in vitro and in vivo, providing a potential tool for noninvasive diagnosis of early thrombi.
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http://dx.doi.org/10.1039/d0bm00729cDOI Listing
August 2020

Stabilization of a full-length infectious cDNA clone for duck Tembusu virus by insertion of an intron.

J Virol Methods 2020 09 26;283:113922. Epub 2020 Jun 26.

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address:

Duck Tembusu virus (DTMUV) belongs to the genus Flavivirus, family Flaviviridae. In our previously study, a full-length cDNA clone of DTMUV was constructed, however, it is prone to mutation during genetic engineering due to the prokaryotic toxicity of viral protein, which is also a common feature for flavivirus. In this study, we reported an intron-containing full-length cDNA clone for a clinical strain CQW1, the intro (133bp) was inserted into nonstructural protein 1 of DTMUV at 192 site. This intron-containing full-length cDNA clone was stably propagated in Escherichia coli without prokaryotic toxicity, and recombinant virus was produced by direct transfection of plasmids. Besides, this cDNA clone-derived recombinant virus showed similar properties in comparison with parent virus both in vitro and in vivo. It's convenient and efficient, making it a useful platform for the subsequent research of reverse genetics of flavivirus.
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http://dx.doi.org/10.1016/j.jviromet.2020.113922DOI Listing
September 2020

Down-regulation of PDGFRβ suppresses invasion and migration in osteosarcoma cells by influencing epithelial-mesenchymal transition.

FEBS Open Bio 2020 09 3;10(9):1748-1757. Epub 2020 Aug 3.

Department of Physiology, Chongqing Medical University, Chongqing, China.

Osteosarcoma (OS) is the most common malignant bone tumor primarily influencing children and adults. Approximately one-fifth of patients have micrometastasis in the lungs when OS is diagnosed. Platelet-derived growth factor receptor (PDGFR) beta (PDGFRβ) is a subtype of PDGFR. PDGFRβ has been noted to be highly expressed in OS cell lines and patient specimens, and is associated with metastasis and poor prognosis of OS. However, mechanistic insights into the exact role of PDGFRβ in OS pathogenesis and development are still lacking. Here we assessed the effects of PDGFRβ on invasive and migratory abilities, such as the epithelial-mesenchymal transition and phosphatidylinositol 3-kinase (PI3K), Akt and mammalian target of rapamycin (mTOR) pathways in HOS cells. Depleting PDGFRβ resulted in reduced migration of HOS cells in the small interfering RNA duplexes specific for the PDGFRβ group compared with the mock and scramble-treated groups in Transwell invasion assays. Using wound-healing assays, we demonstrate the rate of wound healing in the PDGF-BB-stimulated group was higher compared with the mock-treated group. Western blot showed that down-regulation of PDGFRβ decreased the expression of stromal phenotype markers and phosphorylation pathway proteins (PI3K, AKT and mTOR), but the epithelial phenotype marker was increased in HOS cells. Treating HOS cells with PDGF-BB revealed a treatment time-dependent increase of phosphorylated, but not total, PI3K, AKT and mTOR. Taken together, we suggest that PDGFRβ plays an important role in OS invasion, migration and epithelial-mesenchymal transition by influencing the PI3K, Akt and mTOR pathways, hence highlighting PDGFRβ as a potential therapeutic target for OS.
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http://dx.doi.org/10.1002/2211-5463.12915DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459394PMC
September 2020
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