Publications by authors named "Jiao Jiao Li"

90 Publications

Ligand Engineering toward the Trade-off between Stability and Activity in Cluster Catalysis.

Angew Chem Int Ed Engl 2022 Jan 10. Epub 2022 Jan 10.

Tsinghua University, Chemistry Department, 1 Tsinghua Yuan, Haidian District, 100084, Beijing, CHINA.

We report the structures, stability and catalysis properties of two Ag 21 nanoclusters, namely [Ag 21 (H 2 BTCA) 3 (O 2 PPh 2 ) 6 ]SbF 6 ( 1 ) and [Ag 21 (C≡CC 6 H 3 -3,5-R 2 ) 6 (O 2 PPh 2 ) 10 ]SbF 6 ( 2 ) (H 4 BTCA = p - tert -butylthiacalix[4]arene, R = OMe). Both Ag 21 possess identical icosahedral kernel that is surrounded by eight peripheral Ag atoms. Single-crystal structural analysis and ESI-MS revealed that 1 is an 8-electron cluster and 2 has four free electrons. Theoretical results show that the P-symmetry orbitals are found as HOMO-1 and HOMO states in 1 , and the frontier unoccupied molecular orbitals (LUMO, LUMO+1 and LUMO+2) show D-character, indicating 1 is a superatomic cluster with an electronically closed shell 1S 2 1P 6 while 2 has an incomplete shell configuration 1S 2 1P 2 . These two Ag 21 clusters show superior stability under ambient conditions, and 1 is robust even at 90°C in toluene and under oxidative conditions (30% H 2 O 2 ). Significantly, 2 exhibits much higher activity than 1 as catalysts in the reduction of 4-nitrophenol. This work demonstrates that ligands can interfere the electronic structures of silver clusters, and further affect their stability and catalytic performance.
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http://dx.doi.org/10.1002/anie.202116965DOI Listing
January 2022

A 59-Electron Non-Magic-Number Gold Nanocluster Au(C≡CR) Showing Unexpectedly High Stability.

J Am Chem Soc 2022 Jan 7. Epub 2022 Jan 7.

Department of Chemistry, Key Laboratory of Organic Optoelectronics and Molecular Engineering of the Ministry of Education, Tsinghua University, Beijing 100084, P. R. China.

An atomically resolved gold nanocluster Au(C≡CCH-2,4-F) () with an unusual 59 valence electrons has been synthesized. Single-crystal X-ray diffraction reveals that its Au kernel is a Au Marks decahedron capped by two Au units. The surface structure of consists of 20 linear Au(C≡CR) staples. Intercluster interactions are observed between these symmetric clusters. The existence of an unpaired electron is verified by magnetic measurement. Interestingly, this open-shell gold cluster stays intact in toluene solution at 80 °C for more than a week, and it has good charging-discharging capability under electrochemical conditions. The compact ligand shell protection around the symmetric core accounts for the high stability. This work suggests that geometric factors may play a crucial role in determining the stability of a metal nanocluster, even though the cluster has an open-shell electronic structure.
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http://dx.doi.org/10.1021/jacs.1c11643DOI Listing
January 2022

Global Research Trends in Revision Total Knee Arthroplasty: A Bibliometric and Visualized Study.

Indian J Orthop 2021 Oct 10;55(5):1335-1347. Epub 2021 Mar 10.

Arthritis Clinic & Research Center, Peking University People's Hospital, Peking University, Beijing, 100044 China.

Background: Revision total knee arthroplasty (TKA) is a longstanding area of research interest in orthopedics due to its increasing global demand and associated technical challenges. The present study aims to analyze and present the current state of research and trends in this active field.

Methods: Articles on revision TKA published from inception to 2018 were retrieved from Web of Science. Bibliometric analysis was conducted using the metadata of the included articles. Visualized analysis was conducted using VOSviewer software to reveal global trends in revision TKA research, through analyses of bibliographic coupling, co-authorship, co-citation and co-occurrence.

Results: A total of 6027 articles were included. The number of publications and relative research interest in the field of revision TKA displayed strong upward growth over the time period examined. The USA had the highest number of citations for publications in this field, as well as the highest H-index. Studies in the field could be categorized into five clusters: prosthesis design, periprosthetic fracture, periprosthetic joint infection, risk factors for revision TKA, and survivorship of implants. Studies focused on infection and risk factors for revision TKA are likely to become the most popular research topics in the field.

Conclusion: Global trends over the past few years suggest that the field of revision TKA research will continue to grow and lead to increasing rates of publication output over the coming years. Future developments in the field will likely include more preventative and etiological studies relating to revision TKA.
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http://dx.doi.org/10.1007/s43465-021-00390-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586134PMC
October 2021

Nanosurfacing Ti alloy by weak alkalinity-activated solid-state dewetting (AAD) and its biointerfacial enhancement effect.

Mater Horiz 2021 Mar 4;8(3):912-924. Epub 2021 Feb 4.

Research Center for Human Tissues & Organs Degeneration, Shenzhen Institute of Advanced Technology, Chinese Academy of Science, Shenzhen, Guangdong 518055, China.

Nanoscale manipulation of material surfaces can create extraordinary properties, holding great potential for modulating the implant-bio interface for enhanced performance. In this study, a green, simple and biocompatible nanosurfacing approach based on weak alkalinity-activated solid-state dewetting (AAD) was for the first time developed to nano-manipulate the Ti6Al4V surface by atomic self-rearrangement. AAD treatment generated quasi-periodic titanium oxide nanopimples with high surface energy. The nanopimple-like nanostructures enhanced the osteogenic activity of osteoblasts, facilitated M2 polarization of macrophages, and modulated the cross-talk between osteoblasts and macrophages, which collectively led to significant strengthening of in vivo bone-implant interfacial bonding. In addition, the titanium oxide nanopimples strongly adhered to the Ti alloy, showing resistance to tribocorrosion damage. The results suggest strong nano-bio interfacial effects, which was not seen for the control Ti alloy processed through traditional thermal oxidation. Compared to other nanostructuring strategies, the AAD technique shows great potential to integrate high-performance, functionality, practicality and scalability for surface modification of medical implants.
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http://dx.doi.org/10.1039/d0mh01837fDOI Listing
March 2021

Application of extracellular vesicles in the diagnosis and treatment of prostate cancer: Implications for clinical practice.

Crit Rev Oncol Hematol 2021 Nov 13;167:103495. Epub 2021 Oct 13.

School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW, Australia. Electronic address:

Extracellular vesicles (EV) are cell-derived lipid bilayer-delimited structures providing an important means of intercellular communication. Recent studies have shown that EV, particularly exosomes and large-oncosomes contain miRNA and proteins crucial in prostate cancer (PCa) progression, metastasis and treatment resistance. This includes not just EV released from PCa cells, but also from other cells in the tumor microenvironment. PCa patient derived EV have a unique composition compared to healthy and benign prostatic diseases. As such, EV show promise as diagnostic liquid biopsy biomarkers, both as an adjunct and alternative to the invasive current gold-standard. EV could also be utilized to stratify patients' risk and predict response to hormonal, chemo, immune- and targeted therapy, which will direct future treatment decisions in PCa. We present a summary of the current evidence on the role of EV in PCa and the application of EV in PCa diagnosis and treatment to optimize patient outcomes.
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http://dx.doi.org/10.1016/j.critrevonc.2021.103495DOI Listing
November 2021

Molecular Gold Nanocluster Au Showing Metallic Electron Dynamics.

J Am Chem Soc 2021 Oct 5;143(41):17059-17067. Epub 2021 Oct 5.

Department of Chemistry, Key Laboratory of Organic Optoelectronics and Molecular Engineering of the Ministry of Education, Tsinghua University, Beijing 100084, P.R. China.

The boundary between molecular and metallic gold nanoclusters is of special interest. The difficulty in obtaining atomically precise nanoclusters larger than 2 nm limits the determination of such a boundary. The synthesis and total structural determination of the largest all-alkynyl-protected gold nanocluster (PhP)[Au(C≡CR)] (R = 4-CFCH-) () are reported. It presents an ideal platform for studying the relationship between the structure and the metallic nature. has a rod shape with the length and width of the kernel being 2.38 and 2.04 nm, respectively. The cluster contains a concentric Au core structure ([email protected]@Au) protected by 30 linear RC≡C-Au-C≡CR staple motifs. It is interesting that displays multiple excitonic peaks in the steady-state absorption spectrum (molecular) and pump-power-dependent excited-state dynamics as revealed in the transient absorption spectrum (metallic), which indicates that is a critical crossover cluster for the transition from molecular to metallic state. is the smallest-sized gold nanocluster showing metal-like electron dynamics, and it is recognized that the cluster shape is one of the important factors determining the molecular or metallic nature of a gold nanocluster.
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http://dx.doi.org/10.1021/jacs.1c06716DOI Listing
October 2021

A low dose cell therapy system for treating osteoarthritis: study and mechanistic investigations.

Bioact Mater 2022 Jan 2;7:478-490. Epub 2021 Jun 2.

Department of Sports Medicine and Adult Reconstruction Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, 201180, China.

Mesenchymal stem cells (MSCs) can be effective in alleviating the progression of osteoarthritis (OA). However, low MSC retention and survival at the injection site frequently require high doses of cells and/or repeated injections, which are not economically viable and create additional risks of complications. In this study, we produced MSC-laden microcarriers in spinner flask culture as cell delivery vehicles. These microcarriers containing a low initial dose of MSCs administered through a single injection in a rat anterior cruciate ligament (ACL) transection model of OA achieved similar reparative effects as repeated high doses of MSCs, as evaluated through imaging and histological analyses. Mechanistic investigations were conducted using a co-culture model involving human primary chondrocytes grown in monolayer, together with MSCs grown either within 3D constructs or as a monolayer. Co-culture supernatants subjected to secretome analysis showed significant decrease of inflammatory factors in the 3D group. RNA-seq of co-cultured MSCs and chondrocytes using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed processes relating to early chondrogenesis and increased extracellular matrix interactions in MSCs of the 3D group, as well as phenotypic maintenance in the co-cultured chondrocytes. The cell delivery platform we investigated may be effective in reducing the cell dose and injection frequency required for therapeutic applications.
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http://dx.doi.org/10.1016/j.bioactmat.2021.05.029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379370PMC
January 2022

Balanced fertilization over four decades has sustained soil microbial communities and improved soil fertility and rice productivity in red paddy soil.

Sci Total Environ 2021 Nov 24;793:148664. Epub 2021 Jun 24.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; University of Chinese Academy of Sciences, Beijing 100049, China.

The influence of long-term fertilization on soil microbial communities is critical for revealing the association between belowground microbial flora and aboveground crop productivity-a relationship of great importance to food security, environmental protection, and ecosystem functions. Here, we examined shifts in soil chemical properties, microbial communities, and the nutrient uptake and yield of rice subjected to different chemical and organic fertilization treatments over a 40-year period in red paddy soil. Ten different treatments were used: a control without fertilizer, and applications of nitrogen (N), phosphorus (P), potassium (K), NP, NK, PK, NPK, double NPK, or NPK plus manure. Compared with the effects of withholding one or two nutrients (N, P, or K), the balanced application of chemical NPK and organic fertilizers markedly improved soil nutrient status and rice yield. This improvement of soil fertility and rice yield was not associated with bacterial, archaeal, or fungal alpha diversities. The bacterial abundance and community structure and archaeal abundance effectively explained the variation in rice yield, whereas those of fungi did not. The community structure of bacteria and archaea, but not that of fungi, was correlated with soil properties. Among various soil properties, P was the key factor influencing rice yield and soil microbial communities because of the extremely low content of soil available P. Seven keystones at the operational taxonomic unit level were identified: four archaea (belonging to Thermoplasmata, Methanosaeta, Bathyarchaeia, and Nitrososphaeraceae) and three bacteria (in Desulfobacteraceae and Acidobacteriales). These keystones, which were mainly related to soil C and N transformation and pH, may work cooperatively to influence rice yield by regulating soil fertility. Our results collectively suggest that four decades of balanced fertilization has sustained the bacterial and archaeal abundances, bacterial community structure, and keystones, which potentially contribute to soil fertility and rice yield in red paddy soil.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148664DOI Listing
November 2021

Strategies of cadmium and copper uptake and translocation in different plant species growing near an E-waste dismantling site at Wenling, China.

Environ Sci Pollut Res Int 2021 Nov 1;28(44):62562-62571. Epub 2021 Jul 1.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.

This study aimed to explore the interactions between cadmium (Cd) and copper (Cu) during uptake and translocation in plants growing in soil polluted with heavy metals derived from electronic waste (E-waste). We collected the roots, stems, leaves, and root-surrounding soils of ten dominant plant species growing in farmland near an E-waste dismantling site, and analyzed their Cd and Cu concentrations. Among the ten plant species, Echinochloa crus-galli (L.) P. Beauv., Cucurbita moschata (Duch. ex Lam.) Duch. ex Poiret, Phragmites australis (Cav.) Trin. ex Steud., and Benincasa hispida (Thunb.) Cogn. accumulated Cd (2.40-4.56 mg kg) and Cu (19.60-35.21 mg kg) in the roots. In Polygonum hydropiper L. and Sesbania cannabina (Retz.) Poir., the Cd (0.50-0.81 mg kg) and Cu (11.04-15.55 mg kg) concentrations were similar among the three organs. Glycine max (L.) Merr. accumulated more Cu in the roots (16.42 mg kg) than in the stems (5.61 mg kg) and leaves (7.75 mg kg), and accumulated Cd at similar levels in the three organs (0.65-0.99 mg kg). Sesamum indicum L., Bidens pilosa L., and Solidago decurrens Lour. accumulated Cd at similar levels among the three organs (0.16-3.34 mg kg) and accumulated less Cu in the stems (6.89-8.28 mg kg) than in the roots (12.61-21.63 mg kg) and leaves (12.93-22.38 mg kg). S. indicum had a stronger capacity to accumulate and translocate Cd and Cu according to transfer coefficient and translocation factor. The concentrations of Cd and Cu in soils were significantly positively correlated with those in the roots (p<0.01) but not those in the stems and leaves. We detected significantly positive correlations between Cd and Cu concentrations in the roots and leaves (p<0.01) but not in the stems. These results suggest that there is a synergetic strategy of Cd and Cu transport from soils to the roots and from the roots to the leaves, while the stems may not be the key organ controlling Cd and Cu transport in plants. These findings have important implications for the phytoremediation of soils contaminated with Cd and Cu, the mechanisms of plant Cd and Cu transport, and the food safety of agricultural products.
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http://dx.doi.org/10.1007/s11356-021-15072-1DOI Listing
November 2021

Impact of rhizosphere microorganisms on arsenic (As) transformation and accumulation in a traditional Chinese medical plant.

Environ Sci Pollut Res Int 2021 Nov 24;28(43):60923-60934. Epub 2021 Jun 24.

Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Yunnan Provincial Center for Research & Development of Natural Products, School of Chemical Science and Technology, Yunnan University, No. 2 Cuihu North Road, Kunming, 650091, People's Republic of China.

Panax notoginseng is an important traditional medicinal plant, but the commercial value is threatened by root-rot disease caused by rhizosphere microbes and a potential health risk caused by plant arsenic (As) accumulation. Whether rhizospheric microbes isolated from P. notoginseng rhizosphere soil could impact As uptake and transport into P. notoginseng is not yet known. Among the three root-rot disease-causing pathogens Fusarium flocciferum (PG 1), Fusarium oxysporum (PG 2), and Fusarium solani (PG 3) and one root-rot disease biocontrol fungus Trichoderma koningiopsis (FC 1) and five biocontrol-exerting bacterial species Bacillus siamensis (BC 1), Delftia acidovorans (BC 2), Brevibacillus formosus (BC 3), Mortierella alpine (BC 4), and Bacillus subtilis (BC 5), one As-resistant pathogen and four biocontrol microorganisms with As-resistant ability were identified. The As-transforming ability of the identified fungi and bacteria was ranked in the order of FC 1 > PG 1 and BC 2 > BC 3 > BC 1, respectively. Then, the As-resistant biocontrol and pathogenic microbes were initiated to colonize the rhizosphere of 1-year-old P. notoginseng seedlings growing in artificially As(V)-contaminated soil to evaluate the impact of microbe inoculation on P. notoginseng As uptake and transport capacity. Concentration of As in P. notoginseng tissues decreased in the order of the sequence stem > root > leaf. Compared to treatment without colonization by microorganism, inoculation with microorganisms increased As root uptake efficiency and root As concentration, especially under treatment of inoculation by BC 2 and PG 1 + BC 2. As transport efficiency from root to stem decreased by inoculation with microorganism, especially under treatment with inoculation of BC 2 and PG 1 + BC 2. However, the impact of microorganism colonization on As stem to leaf transport efficiency was not obvious. In summary, inoculation with rhizosphere microbes may increase As accumulation in P. notoginseng root, especially when using bacteria with high As transformation ability. Therefore, it is necessary to evaluate the As transformation capacity before applying biological control microorganism to the rhizosphere of P. notoginseng.
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http://dx.doi.org/10.1007/s11356-021-14500-6DOI Listing
November 2021

Single-cell multi-omics sequencing: application trends, COVID-19, data analysis issues and prospects.

Brief Bioinform 2021 11;22(6)

Data Science Institute, University of Technology Sydney, Ultimo, NSW 2007, Australia.

Single-cell sequencing is a biotechnology to sequence one layer of genomic information for individual cells in a tissue sample. For example, single-cell DNA sequencing is to sequence the DNA from every single cell. Increasing in complexity, single-cell multi-omics sequencing, or single-cell multimodal omics sequencing, is to profile in parallel multiple layers of omics information from a single cell. In practice, single-cell multi-omics sequencing actually detects multiple traits such as DNA, RNA, methylation information and/or protein profiles from the same cell for many individuals in a tissue sample. Multi-omics sequencing has been widely applied to systematically unravel interplay mechanisms of key components and pathways in cell. This survey overviews recent developments in single-cell multi-omics sequencing, and their applications to understand complex diseases in particular the COVID-19 pandemic. We also summarize machine learning and bioinformatics techniques used in the analysis of the intercorrelated multilayer heterogeneous data. We observed that variational inference and graph-based learning are popular approaches, and Seurat V3 is a commonly used tool to transfer the missing variables and labels. We also discussed two intensively studied issues relating to data consistency and diversity and commented on currently cared issues surrounding the error correction of data pairs and data imputation methods. The survey is concluded with some open questions and opportunities for this extraordinary field.
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http://dx.doi.org/10.1093/bib/bbab229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8344433PMC
November 2021

Infectious disease mRNA vaccines and a review on epitope prediction for vaccine design.

Brief Funct Genomics 2021 09;20(5):289-303

Data Science Institute, Faculty of Engineering & IT, University of Technology Sydney, 15 Broadway, Ultimo, 2007, New South Wales, Australia.

Messenger RNA (mRNA) vaccines have recently emerged as a new type of vaccine technology, showing strong potential to combat the COVID-19 pandemic. In addition to SARS-CoV-2 which caused the pandemic, mRNA vaccines have been developed and tested to prevent infectious diseases caused by other viruses such as Zika virus, the dengue virus, the respiratory syncytial virus, influenza H7N9 and Flavivirus. Interestingly, mRNA vaccines may also be useful for preventing non-infectious diseases such as diabetes and cancer. This review summarises the current progresses of mRNA vaccines designed for a range of diseases including COVID-19. As epitope study is a primary component in the in silico design of mRNA vaccines, we also survey on advanced bioinformatics and machine learning algorithms which have been used for epitope prediction, and review on user-friendly software tools available for this purpose. Finally, we discuss some of the unanswered concerns about mRNA vaccines, such as unknown long-term side effects, and present with our perspectives on future developments in this exciting area.
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http://dx.doi.org/10.1093/bfgp/elab027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194884PMC
September 2021

A stable well-defined copper hydride cluster consolidated with hemilabile phosphines.

Chem Commun (Camb) 2021 Apr;57(35):4315-4318

Department of Chemistry, Tsinghua University, Beijing, 100084, P. R. China.

Copper hydrides are very useful in hydrogenation reactions. We report a stable Stryker-type copper hydride reagent protected by hemilabile phosphines: [Cu8H6(dppy)6](OTf)2 (Cu8-H, dppy = diphenylphosphino-2-pyridine). The metal core of this cluster has a bicapped octahedral configuration, and the copper-bound hydrides each triply bridges over a triangular face of the octahedron. This cluster is attractive due to its facile preparation and excellent stability under ambient conditions. The comparable activity and selectivity both in the stoichiometric and catalytic reactions make Cu8-H a promising alternative to Stryker's reagent.
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http://dx.doi.org/10.1039/d1cc01161hDOI Listing
April 2021

Cluster From Cluster: A Quantitative Approach to Magic Gold Nanoclusters [Au (SR) ].

Angew Chem Int Ed Engl 2021 Jun 14;60(26):14415-14419. Epub 2021 May 14.

Department of Chemistry, Key Laboratory of Organic Optoelectronics and Molecular Engineering of the Ministry of Education, Tsinghua University, Beijing, 100084, P. R. China.

High-yield and large-scale synthesis are highly demanded for the studies of gold nanoclusters. We developed a "cluster from cluster" approach to assemble gold nanoclusters with preformed atomically precise Au precursors. This facile approach has proved to be very effective in the synthesis of the well-known magic cluster [Au (SR) ] , which could prepare the target cluster in high yield (overall yield up to ≈100 %) at large scale (gram-scale based on gold). This method can be applied in the synthesis of 10 Au clusters with different R groups. This work presents an important approach that may be extended to high-yield and large-scale synthesis of other metal nanoclusters.
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http://dx.doi.org/10.1002/anie.202103290DOI Listing
June 2021

Extracellular Vesicles from Mesenchymal Stromal Cells for the Treatment of Inflammation-Related Conditions.

Int J Mol Sci 2021 Mar 16;22(6). Epub 2021 Mar 16.

Kolling Institute, Faculty of Medicine and Health, University of Sydney, St. Leonards, NSW 2065, Australia.

Over the past two decades, mesenchymal stromal cells (MSCs) have demonstrated great potential in the treatment of inflammation-related conditions. Numerous early stage clinical trials have suggested that this treatment strategy has potential to lead to significant improvements in clinical outcomes. While promising, there remain substantial regulatory hurdles, safety concerns, and logistical issues that need to be addressed before cell-based treatments can have widespread clinical impact. These drawbacks, along with research aimed at elucidating the mechanisms by which MSCs exert their therapeutic effects, have inspired the development of extracellular vesicles (EVs) as anti-inflammatory therapeutic agents. The use of MSC-derived EVs for treating inflammation-related conditions has shown therapeutic potential in both in vitro and small animal studies. This review will explore the current research landscape pertaining to the use of MSC-derived EVs as anti-inflammatory and pro-regenerative agents in a range of inflammation-related conditions: osteoarthritis, rheumatoid arthritis, Alzheimer's disease, cardiovascular disease, and preeclampsia. Along with this, the mechanisms by which MSC-derived EVs exert their beneficial effects on the damaged or degenerative tissues will be reviewed, giving insight into their therapeutic potential. Challenges and future perspectives on the use of MSC-derived EVs for the treatment of inflammation-related conditions will be discussed.
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http://dx.doi.org/10.3390/ijms22063023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002312PMC
March 2021

Efficacy and safety of COVID-19 vaccines: a systematic review.

Zhongguo Dang Dai Er Ke Za Zhi 2021 Mar;23(3):221-228

Department of Pediatrics, Renmin Hospital of Wuhan University, Wuhan 430060, China.

Objective: To evaluate systematically the efficacy and safety of COVID-19 vaccines.

Methods: PubMed, Embase, Cochrane Library, Clinicaltrial.gov, CNKI, Wanfang Data, China Biomedical Literature Service System, and China Clinical Trial Registry were searched for randomized controlled trials of COVID-19 vaccines published up to December 31, 2020. The Cochrane bias risk assessment tool was used to assess the quality of studies. A qualitative analysis was performed on the results of clinical trials.

Results: Thirteen randomized, blinded, controlled trials, which involved the safety and efficacy of 11 COVID-19 vaccines, were included. In 10 studies, the 28-day seroconversion rate of subjects exceeded 80%. In two 10 000-scale clinical trials, the vaccines were effective in 95% and 70.4% of the subjects, respectively. The seroconversion rate was lower than 60% in only one study. In six studies, the proportion of subjects who had an adverse reaction within 28 days after vaccination was lower than 30%. This proportion was 30%-50% in two studies and > 50% in the other two studies. Most of the adverse reactions were mild to moderate and resolved within 24 hours after vaccination. The most common local adverse reaction was pain or tenderness at the injection site, and the most common systemic adverse reaction was fatigue, fever, or bodily pain. The immune response and incidence of adverse reactions to the vaccines were positively correlated with the dose given to the subjects. The immune response to the vaccines was worse in the elderly than in the younger population. In 6 studies that compared single-dose and double-dose vaccination, 4 studies showed that double-dose vaccination produced a stronger immune response than single-dose vaccination.

Conclusions: Most of the COVID-19 vaccines appear to be effective and safe. Double-dose vaccination is recommended. However, more research is needed to investigate the long-term efficacy and safety of the vaccines and the influence of dose, age, and production process on the protective efficacy.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969187PMC
March 2021

Clinical-Grade Human Embryonic Stem Cell-Derived Mesenchymal Stromal Cells Ameliorate the Progression of Osteoarthritis in a Rat Model.

Molecules 2021 Jan 24;26(3). Epub 2021 Jan 24.

Arthritis Clinic & Research Center, Peking University People's Hospital, Peking University, Beijing 100044, China.

Mesenchymalstem cell (MSC)-based therapy is being increasingly explored in preclinical and clinical studies as a regenerative method for treating osteoarthritis (OA). However, the use of primary MSCs is hampered by a number of limitations, including donor heterogeneity and inconsistent cell quality. Here, we tested the therapeutic potential of embryonic stem cell-derived MSCs (ES-MSCs) in anOA rat model. ES-MSCs were generated and identified by morphology, trilineage differentiation and flow cytometry. Sprague Dawley rats were treated with either a single dose (10 cells/rat) of ES-MSCs or with three doses spaced one week apart for each dose, starting at four weeks after anterior cruciate ligament transectionto induce OA. Cartilage quality was evaluated at 6 and 10 weeks after treatment with behavioral analysis, macroscopic examination, and histology. At sixweeks after treatment, the groups treated with both single and repeated doses of ES-MSCs had significantly better modified Mankin scores and International Cartilage Repair Society (ICRS) macroscopic scores in the femoral condyle compared to the control group. At 10 weeks after treatment, the repeated doses group had a significantly better ICRS macroscopic scores in the femoral condyle compared to the single dose and control groups. Histological analysis also showed more proteoglycan and less cartilage loss, along with lower Mankin scores in the repeated doses group. In conclusion, treatment with multiple injections of ES-MSCs can ameliorate OA in a rat model. TheES-MSCs have potential to be considered as a regenerative therapy for OA, and can provide an infinite cellular source.
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http://dx.doi.org/10.3390/molecules26030604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865331PMC
January 2021

Anti-inflammatory constituents in the root and rhizome of Polygonum cuspidatum by UPLC-PDA-QTOF/MS and lipopolysaccharide-activated RAW264.7 macrophages.

J Pharm Biomed Anal 2021 Feb 26;195:113839. Epub 2020 Dec 26.

The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China. Electronic address:

The root and rhizome of Polygonum cuspidatum (Hu-Zhang) has been used for treatment of various inflammatory disorders in China. In our pervious study, we found that three fractions (HZE-30, HZE-60 and HZE-95) from the ethanol extract of Hu-Zhang (HZE) all could inhibit NO production, and HZE-60 shows the most potent anti-inflammatory activity. In order to understand the major contribution constituents of Hu-Zhang responsible for its anti-inflammatory effect, quantitative composition-activity relationship method was performed. Firstly, the constituents in HZE-60 were characterized using an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) approach. Second, quantitative analyzed five major constituents identified in HZE-60 and compare the difference of five major constituents in HZE and three anti-inflammatory activity fractions. Finally, evaluated the anti-inflammatory effects of major constituents in lipopolysaccharide (LPS)-activated RAW264.7 macrophages. The results showed that a total of 31 compounds were identified from HZE-60, including 12 anthraquinones, 7 diphenylethenes, 9 phenols and 3 others. The contents of five major constituents (polydatin (6), resveratrol (7), emodin-1-O-β-d-glucoside (15), emodin-8-O-β-d-glucoside (21) and emodin (31)) were simultaneously determined by UPLC-PDA with good linearity (correlation coefficients > 0.9990) and satisfactory repeatability (RSD < 0.99 %), precision (RSD < 0.01 %), stability (RSD < 0.67 %) and recoveries (99.52 %-101.23 %, RSD < 0.91 %). All five major constituents could be detected in HZE and HZE-60 fraction, but only 6 was detected in HZE-30, and 31 in HZE-95. Moreover, 7, 15 and 21 exhibited significant anti-inflammatory activity via suppressing supernatant pro-inflammatory mediators, such as NO, tumor Necrosis Factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1). Therefore, we conclude that the bioactivity of HZE is the syngeneic effect of its constituents, and 7, 15 and 21 should make great contributions for the anti-inflammatory effect of Hu-Zhang. The findings define the anti-inflammatory chemical constituents of Hu-Zhang, which will benefit further investigation on its quality control and the mechanism of action.
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http://dx.doi.org/10.1016/j.jpba.2020.113839DOI Listing
February 2021

[Bushen Huoxue Recipe reduces cyclophosphamide-induced apoptosis of spermatogenic cells in mice].

Zhonghua Nan Ke Xue 2020 Sep;26(9):826-831

Shanxi University of Chinese Medicine, Xianyang, Shaanxi 712046, China.

Objective: To investigate the effect of Bushen Huoxue Recipe (BHR) on cyclophosphamide-induced apoptosis of testicular spermatogenic cells in mice and its possible action mechanisms.

Methods: Fifty male Babl/c mice aged 8-9 weeks were randomly divided into five groups of an equal number: blank control, model control, low-dose BHR, medium-dose BHR and high-dose BHR. The animals in the blank control group were intraperitoneally injected with normal saline, while those in the other four groups with cyclophosphamide at 50 mg/kg/d, all for 7 days. After modeling, the mice in the blank and model control groups were given distilled water via gavage once a day, and those in the low-, medium- and high-dose BHR groups treated intragastrically with BHR at 7.5, 15 and 30 g/kg/d qd for 30 successive days. Then, the apoptosis index of the testicular spermatogenic cells was obtained by TUNEL and the expressions of Bax and Bcl-2 mRNA and proteins determined by RT-PCR and Western blot, respectively.

Results: Compared with the mice in the blank control group, the BHR model controls showed dramatically increased apoptosis of testicular spermatogenic cells and up-regulated mRNA and protein expressions of Bax and Bcl-2 in the testis tissue (P < 0.01). In comparison with the model controls, the mice in the BHR treatment groups exhibited significantly reduced apoptosis of testicular spermatogenic cells and down-regulated mRNA and protein expressions of Bax and Bcl-2 in the testis tissue (P < 0.01).

Conclusions: Bushen Huoxue Recipe can reduce cyclophosphamide-induced apoptosis of testicular spermatogenic cells in mice, which may be associated with its ability of regulating the expressions of Bax and Bcl-2 mRNA and proteins in the testis tissue.
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September 2020

[Ecological significance of arbuscular mycorrhiza on plant rhizosphere stress].

Ying Yong Sheng Tai Xue Bao 2020 Sep;31(9):3216-3226

College of Horticulture and Landscape, Southwest University, Chongqing 400715, China.

In recent years, Chinese scientists have made remarkable achievements in on mycorrhizal molecular biology, nutrition, taxonomy, and ecology, with arbuscular mycorrhizal fungus (AMF) having been mostly studied. AMF can form symbiotic relationship with roots of most terrestrial plants, promote plant growth and development, improve plant stress resistance, maintain ecological balance, and protect ecological environment. This review mainly introduced the ecological function and mechanism of AMF in plant rhizosphere stress from the aspects of abiotic (drought stress, heavy metal pollution, saline-alkali stress) and biotic stresses (pathogenic bacteria and nematode infection). We proposed the remaining deficiencies and research prospects in this field to provide refe-rence for future research of AMF.
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http://dx.doi.org/10.13287/j.1001-9332.202009.039DOI Listing
September 2020

Enriching Structural Diversity of Alkynyl-Protected Gold Nanoclusters with Chlorides.

Angew Chem Int Ed Engl 2021 Mar 11;60(12):6699-6703. Epub 2021 Feb 11.

Department of Chemistry, Key Laboratory of Organic Optoelectronics and Molecular Engineering of the Ministry of Education, Tsinghua University, Beijing, 100084, P. R. China.

The synthesis and isolation of alkynyl/chloride-protected gold nanoclusters is described. Silica gel column chromatography is effective in isolating gold nanoclusters from the as-synthesized cluster mixture to give the clusters Na[Au L ] (Au ), [HNEt ] [Au L Cl ] (Au ), [HNEt ] [Au L Cl ] (Au ), L=3,5-bis(trifluoromethyl)-phenylacetylide. Au and Au are new clusters; the structures were determined by X-ray single-crystal diffraction. Au contains a distorted Au Marks decahedron shelled by an irregular Au and further protected with two V-shaped Au L , 13 linear AuL staples and 4 chlorides. Au is the first structurally determined 34e superatomic gold nanocluster. Au is composed of 106 Au atoms co-protected by alkynyls and chlorides. It has a Au kernel, like in Au (p-MBA) . The surface structure of Au includes 20 linear Au-alkynyl staples, 5 Cl-Au-Cl and 2 Cl-Au motifs. These three gold nanoclusters show size-dependent electrochemical properties.
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http://dx.doi.org/10.1002/anie.202014154DOI Listing
March 2021

Topical Diclofenac Solution for Osteoarthritis of the Knee: An Updated Meta-Analysis of Randomized Controlled Trials.

Biomed Res Int 2020 24;2020:1758071. Epub 2020 Nov 24.

Department of Pharmacy, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

This study was performed to assess the efficacy and safety of a topical diclofenac solution in patients with knee osteoarthritis (OA). PubMed, Embase, Cochrane Library, Web of Science, and Scopus databases were searched for randomized controlled trials until June 2020. The WOMAC pain, stiffness, physical function subscales, pain on walking, and the occurrence of adverse events were pooled to comprehensively analyse the efficacy and safety of topical diclofenac solution. All statistical analyses were conducted using Review Manager 5.3 software. Five RCTs were included, which provided high-quality evidence. In comparison to the vehicle control, the mean differences for WOMAC pain, stiffness, and physical function subscales, as well as pain on walking, were all statistically significant in favor of topical diclofenac solution. The safety of topical diclofenac solution was similar to the vehicle control, apart from adverse events involving application-site skin reactions. Topical diclofenac solution is effective and safe for use in patients with knee OA, but may cause minor skin reactions.
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http://dx.doi.org/10.1155/2020/1758071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707945PMC
May 2021

Animal Models for Treating Spinal Cord Injury Using Biomaterials-Based Tissue Engineering Strategies.

Tissue Eng Part B Rev 2021 Mar 5. Epub 2021 Mar 5.

Department of Orthopedics and Shanxi Medical University Second Affiliated Hospital, Taiyuan, China.

The aim of the study is to provide an up-to-date review of studies that used preclinical animal models for the evaluation of tissue engineering treatments for spinal cord injury (SCI), which involved the use of biomaterials with or without the addition of cells or biomolecules. Electronic search of the PubMed, Web of Science, and Embase databases was performed for relevant studies published between January 2009 and December 2019. In total, 1579 articles were retrieved, of which 58 studies were included for analysis. Among the included studies, rats were the most common species used for animal models of SCI, while complete transection was the most commonly used injury pattern. Immediate intervention after injury was conducted in the majority of studies, and 8 weeks was the most common final time point of outcome assessment. A wide range of natural and synthetic biomaterials with different morphologies were used as a part of tissue engineering treatments for SCI, including scaffolds, hydrogels, and particles. Experimental parameters in studies using SCI animal models to evaluate tissue engineering treatments should be carefully considered to match the purpose of the study. Biomaterials that have functional modifications or are applied in combination with cells and biomolecules can be effective in creating a permissive environment for SCI repair in preclinical animal models.
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http://dx.doi.org/10.1089/ten.TEB.2020.0267DOI Listing
March 2021

MicroRNA-1 Modulates Chondrocyte Phenotype by Regulating FZD7 of Wnt/ β-Catenin Signaling Pathway.

Cartilage 2021 12 20;13(2_suppl):1019S-1029S. Epub 2020 Nov 20.

Kolling Institute, Faculty of Medicine and Health, University of Sydney, St. Leonards, New South Wales, Australia.

Objective: Osteoarthritis (OA) is an incurable joint disease characterized by pronounced pain. MicroRNAs constitute epigenetic mechanisms that may affect OA progression by contributing to changes in chondrocyte phenotype. This study investigates for the first time whether there is a link between miRNA-1 (miR-1) and OA pathogenesis, and the molecular mechanisms involved.

Design: OA-associated gene expression, including MMP-13, ADAMTS5, and COL2A1 was compared in chondrocytes from non-OA and OA cartilage, and in SW1353 cells over- and underexpressing miR-1. Bioinformatics and luciferase reporter assay were conducted to confirm whether FZD7 was a target of miR-1. The effects of miR-1 on FZD7 expression and downstream Wnt/β-catenin signalling were investigated.

Results: Non-OA and OA chondrocytes differed significantly in the expression of miR-1 and OA-associated genes. MiR-1 over- and underexpression in SW1353 cells, respectively, reduced and enhanced gene expression associated with cartilage catabolism. FZD7, which has an important role in the Wnt/β-catenin signaling pathway, was shown to be a potential target of miR-1. MiR-1 binding to FZD7 increased the levels of phosphorylated (inactivated) β-catenin, thereby preventing downstream β-catenin signaling.

Conclusions: Inhibition of Wnt/β-catenin signaling by miR-1 in chondrocytes may attenuate the expression of genes that regulate the activity of catabolic enzymes. This finding may be useful for future investigations of molecular targets for OA treatment.
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http://dx.doi.org/10.1177/1947603520973255DOI Listing
December 2021

Risk Factors with Multilevel Evidence for Dislocation in Patients with Femoral Neck Fractures After Hip Hemiarthroplasty: A Systematic Review.

Indian J Orthop 2020 Nov 2;54(6):795-804. Epub 2020 Jul 2.

Department of Orthopedic, Second Hospital of Shanxi Medical University, Taiyuan, China.

Background: Hip hemiarthroplasty (HA) is a standard surgical procedure for elderly patients with displaced fracture of the femoral neck, where dislocation is a possible complication. This study is a systematic review on the risk factors of implant dislocation in patients with femoral neck fracture following hip hemiarthroplasty (HA), and evaluates the methodological quality of the included studies.

Methods: Studies on risk factor assessment of dislocation following hip HA were sourced from EMBASE, Ovid, PubMed and ScienceDirect databases. The quality of included studies was evaluated using an improved quality evaluation method combined with a best-evidence synthesis method.

Results: A total of 130,127 patients were involved in 17 observational studies included in this systematic review, with a dislocation rate that ranged between 0.76 and 12.2% (overall incidence was 4-5% by meta-analysis). According to the applied quality evaluation criteria, eight studies were considered to be of high quality, six to be of medium quality, and three to be of low quality. The posterolateral surgical approach was identified as the only risk factor supported by strong evidence, while patients with small acetabular coverage and low postoperative offset were identified as risk factors supported by moderate evidence, and 11 other risk factors were supported by limited evidence.

Conclusion: This systematic review provides some evidence in helping surgeons develop optimal prevention strategies for dislocation following hip HA during the perioperative period based on common risk factors identified in the literature. However, conclusive evidence supporting most of these risk factors is lacking and more methodologically rigorous studies are required to increase the confidence of recommendations.
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http://dx.doi.org/10.1007/s43465-020-00177-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573085PMC
November 2020

New technology-based assistive techniques in total knee arthroplasty: A Bayesian network meta-analysis and systematic review.

Int J Med Robot 2020 Oct 27:e2189. Epub 2020 Oct 27.

Arthritis Clinic & Research Center, Peking University People's Hospital, Peking University, Beijing, China.

Background: The radiological and clinical efficiency among robot-assisted surgery (RAS), computer-assisted navigation system (CAS) and conventional (CON) total knee arthroplasty (TKA) remains controversial.

Methods: Bayesian network meta-analysis (NMA) and systematic review were performed to investigate radiological and clinical efficiency respectively. The certainty of the evidence was evaluated using GRADE and CERQual tool.

Results: Thirty-four RCTs (7289 patients and 7424 knees) were included. The NMA showed that RAS-TKA had the highest probability for mechanical axis restoration (odds ratio for RAS vs. CAS 3.79, CrI 1.14 to 20.54, very low certainty), followed by CAS-TKA (odds ratio for CAS vs. CON 2.55, CrI 1.67 to 4.01, very low certainty) and then CON-TKA, without significant differences in other radiological parameters. No differences were found in clinical outcomes after qualitative systematic review (overall low certainty).

Conclusions: Technology-based assistive techniques (CAS and RAS) may surpass the CON-TKA, when considering higher radiological accuracy and comparable clinical outcomes. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/rcs.2189DOI Listing
October 2020

Large autologous ilium with periosteum for tibiotalar joint reconstruction in Rüedi-Allgöwer III or AO/OTA type C3 pilon fractures: a pilot study.

BMC Musculoskelet Disord 2020 Sep 25;21(1):632. Epub 2020 Sep 25.

Department of Orthopedic, Second Hospital of Shanxi Medical University, Taiyuan, China.

Background: Management of Rüedi-Allgöwer III or AO/OTA type C3 pilon fracture presents numerous challenges to the orthopaedic surgeon. A joint preservation technique using a large autologous ilium with periosteum in combination with internal implant fixation was reported to improve the outcome of reconstruction.

Methods: Twenty-five patients according to Tscherne/Oestern FxCO-I closed fracture and FxOI open fractures classification after Rüedi-Allgöwer III or AO/OTA type C3 pilon fracture received a large autologous ilium with periosteum for tibiotalar joint reconstruction and open reduction and internal fixation (ORIF), between March 2015 and September 2018. The visual analog scale (VAS), American Orthopaedic Foot & Ankle Society (AOFAS) score, and Burwell and Charnley criteria were used for outcome analysis.

Results: Twenty patients with an average age of 45.2 years were followed for an average of 18.3 months. The VAS and AOFAS scores, and Burwell and Charnley ratings were recorded at the last follow-up after reconstructive surgery. Two patients developed redness and swelling at the wound site, but recovered after local care and dressing changes. No patient displayed deep surgical site infection, donor site complication, non-union or local complication during the final follow-up. The average bone union time was 18.3 months (range 3-36).

Conclusions: Large autologous ilium with periosteum in combination with ORIF can be performed for tibiotalar joint reconstruction. This experimental procedure reduces the risk of post-operative complications following articular reconstruction for Rüedi-Allgöwer III or AO/OTA type C3 pilon fractures in short follow-up.

Level Of Evidence: Level III, retrospective cohort study.
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http://dx.doi.org/10.1186/s12891-020-03659-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7519531PMC
September 2020

Introducing nitrogen atoms to amidoalkylindoles: potent and selective cannabinoid type 2 receptor agonists with improved aqueous solubility.

Medchemcomm 2019 Dec 18;10(12):2131-2139. Epub 2019 Nov 18.

Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development , School of Chemistry and Molecular Engineering , East China Normal University , 3663 North Zhongshan Road , Shanghai 200062 , China . Email:

Previously we identified a series of amidoalkylindoles as potent and selective CB partial agonists. In the present study, we report our continuous effort to improve the aqueous solubility by introducing N atoms to the amidoalkylindole framework. Synthesis, characterization, and pharmacology evaluations were described. Bioisosteric replacements of the indole nucleus with an indazole, azaindole and benzimidazole were explored. Benzimidazole (EC = NA, EC = 0.067 μM) and azaindole (EC = NA, EC = 0.048 μM) were found to be potent and selective CB receptor partial agonists, both with improved aqueous solubility.
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http://dx.doi.org/10.1039/c9md00411dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7451064PMC
December 2019

Consistent apparent Young's modulus of human embryonic stem cells and derived cell types stabilized by substrate stiffness regulation promotes lineage specificity maintenance.

Cell Regen 2020 Sep 3;9(1):15. Epub 2020 Sep 3.

Department of Biomedical Engineering, Tsinghua-Peking Center for Life Sciences, MOE Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, School of Medicine, Tsinghua University, Beijing, 100084, China.

Background: Apparent Young's modulus (AYM), which reflects the fundamental mechanical property of live cells measured by atomic force microscopy and is determined by substrate stiffness regulated cytoskeletal organization, has been investigated as potential indicators of cell fate in specific cell types. However, applying biophysical cues, such as modulating the substrate stiffness, to regulate AYM and thereby reflect and/or control stem cell lineage specificity for downstream applications, remains a primary challenge during in vitro stem cell expansion. Moreover, substrate stiffness could modulate cell heterogeneity in the single-cell stage and contribute to cell fate regulation, yet the indicative link between AYM and cell fate determination during in vitro dynamic cell expansion (from single-cell stage to multi-cell stage) has not been established.

Results: Here, we show that the AYM of cells changed dynamically during passaging and proliferation on substrates with different stiffness. Moreover, the same change in substrate stiffness caused different patterns of AYM change in epithelial and mesenchymal cell types. Embryonic stem cells and their derived progenitor cells exhibited distinguishing AYM changes in response to different substrate stiffness that had significant effects on their maintenance of pluripotency and/or lineage-specific characteristics. On substrates that were too rigid or too soft, fluctuations in AYM occurred during cell passaging and proliferation that led to a loss in lineage specificity. On a substrate with 'optimal' stiffness (i.e., 3.5 kPa), the AYM was maintained at a constant level that was consistent with the parental cells during passaging and proliferation and led to preservation of lineage specificity. The effects of substrate stiffness on AYM and downstream cell fate were correlated with intracellular cytoskeletal organization and nuclear/cytoplasmic localization of YAP.

Conclusions: In summary, this study suggests that optimal substrate stiffness regulated consistent AYM during passaging and proliferation reflects and contributes to hESCs and their derived progenitor cells lineage specificity maintenance, through the underlying mechanistic pathways of stiffness-induced cytoskeletal organization and the downstream YAP signaling. These findings highlighted the potential of AYM as an indicator to select suitable substrate stiffness for stem cell specificity maintenance during in vitro expansion for regenerative applications.
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http://dx.doi.org/10.1186/s13619-020-00054-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467757PMC
September 2020

Interference screws are more likely to perform better than cortical button and cross-pin fixation for hamstring autograft in ACL reconstruction: a Bayesian network meta-analysis.

Knee Surg Sports Traumatol Arthrosc 2021 Jun 19;29(6):1850-1861. Epub 2020 Aug 19.

Department of Orthopaedic Surgery, Shanxi Medical University Second Affiliated Hospital, Taiyuan, China.

Purpose: Anterior cruciate ligament (ACL) reconstruction is widely accepted as the first choice of treatment for ACL injury, but there is disagreement in the literature regarding the optimal femoral fixation method. This meta-analysis assesses the evidence surrounding three common femoral fixation methods: cortical button (CB), cross-pin (CP) and interference screws (IS).

Methods: A systematic search was conducted in Medline, EMBASE and the Cochrane Library to identify studies with evidence level I or II that compared at least two femoral fixation methods with hamstring autograft for ACL reconstruction. Ten primary outcomes were collected. Risk of bias was assessed following the Cochrane Handbook for Systematic Reviews of Interventions. Standardized mean differences (SMD) were estimated using random-effects network meta-analysis in a Bayesian framework. Probability of ranking best (ProBest) and surface under the cumulative ranking curve (SUCRA) were used to rank all treatments. Funnel plots were used to identify publication bias and small-study effects.

Results: Sixteen clinical trials were included for analysis out of 2536 retrieved studies. Bayesian network meta-analysis showed no significant differences among the three fixation methods for the ten primary outcome measures. Based on the 10 outcome measures, the IS, CB and CP had the highest ProBest in 5, 5 and 0 outcomes, and the highest SUCRA values in 5, 4 and 1 outcomes, respectively. No substantial inconsistency between direct and indirect evidence, or publication bias was detected in the outcomes.

Conclusion: There were no statistical differences in performance among the CP, CB and IS femoral fixation methods with hamstring autograft in ACL reconstruction, although the IS was more likely to perform better than CB and CP based on the analysis of outcome measures from the included studies.

Level Of Evidence: 1.
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http://dx.doi.org/10.1007/s00167-020-06231-xDOI Listing
June 2021
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