Publications by authors named "Jiao Jiao"

162 Publications

Metal-Coordinated Supramolecular Self-Assemblies for Cancer Theranostics.

Adv Sci (Weinh) 2021 Jun 18:e2101101. Epub 2021 Jun 18.

Key Laboratory of Forest Plant Ecology, Ministry of Education, College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, 150040, P. R. China.

Metal-coordinated supramolecular nanoassemblies have recently attracted extensive attention as materials for cancer theranostics. Owing to their unique physicochemical properties, metal-coordinated supramolecular self-assemblies can bridge the boundary between traditional inorganic and organic materials. By tailoring the structural components of the metal ions and binding ligands, numerous multifunctional theranostic nanomedicines can be constructed. Metal-coordinated supramolecular nanoassemblies can modulate the tumor microenvironment (TME), thus facilitating the development of TME-responsive nanomedicines. More importantly, TME-responsive organic-inorganic hybrid nanomaterials can be constructed in vivo by exploiting the metal-coordinated self-assembly of a variety of functional ligands, which is a promising strategy for enhancing the tumor accumulation of theranostic molecules. In this review, recent advancements in the design and fabrication of metal-coordinated supramolecular nanomedicines for cancer theranostics are highlighted. These supramolecular compounds are classified according to the order in which the coordinated metal ions appear in the periodic table. Furthermore, the prospects and challenges of metal-coordinated supramolecular self-assemblies for both technical advances and clinical translation are discussed. In particular, the superiority of TME-responsive nanomedicines for in vivo coordinated self-assembly is elaborated, with an emphasis on strategies that enhance the accumulation of functional components in tumors for an ideal theranostic outcome.
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http://dx.doi.org/10.1002/advs.202101101DOI Listing
June 2021

N6-Methyladenosine-Related RNA Signature Predicting the Prognosis of Ovarian Cancer.

Recent Pat Anticancer Drug Discov 2021 Jun 15. Epub 2021 Jun 15.

The Research Center for Medical Genomics, School of Life Sciences, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang City, 110122, Liaoning, China.

Background: N6-Methyladenosine (m6A) RNA methylation is the most universal mRNA modification in eukaryotic cells. M6A mRNA modification affects almost every phases of RNA processing, including splicing, decay, export, translation and expression. Several patents have reported the application of m6A mRNA modification in cancer diagnosis and treatment. Ovarian cancer is the leading cause of death among all gynecological cancers. It is urgent to identify new biomarkers for early diagnosis and prognosis of ovarian cancer.

Objective: In the current study, we aimed to evaluate the m6A RNA methylation regulators and m6A related genes and establish a new gene signature panel for prognosis of ovarian cancer.

Method: We downloaded the Mutations data, FPKM data and corresponding clinical information of 373 patients with ovarian cancer (OC) from the TCGA database. We performed LASSO regression analysis and multivariate cox regression analysis to develop a risk-identifying gene signature panel.

Results: A total of 317 candidate m6A RNA methylation related genes were obtained. Finally, 12 -genes (WTAP, LGR6, ZC2HC1A, SLC4A8, AP2A1, NRAS, CUX1, HDAC1, CD79A, ACE2, FLG2 and LRFN1) were selected to establish the signature panel. We analyzed the genetic alterations of the selected 12 -genes in OC using cBioPortal database. Among the 373 patients, 368 patients have mutations. The results showed that all queried genes were altered in 137 of 368 cases (37.23%). The 12-gene signature panel was confirmed as an independent prognostic indicator (P =2.29E-18, HR = 1.699, 95% CI = 1.508-1.913).

Conclusion: We established an effective m6A-related gene signature panel consisted of 12 -genes, which can predict the outcome of patients with OC. The high risk score indicates unfavorable survival. Our study provided novel insights into the relationship between m6A and OC. This gene signature panel will be helpful in identifying poor prognostic patients with OC and could be a promising prognostic indicator in clinical practice.
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http://dx.doi.org/10.2174/1574892816666210615164645DOI Listing
June 2021

Facile and simple fabrication of superhydrophobic and superoleophilic MS/PDA/DT sponge for efficient oil/water separation.

Environ Technol 2021 Jun 11:1-17. Epub 2021 Jun 11.

(School of Chemical Engineering, Xi'an University, Xi'an 710065 Shaanxi, PR China.).

In order to overcome the problems of frequent leakage accidents during oil exploitation, a superhydrophobic and superoleophilic porous MS/PDA/DT sponge was successfully prepared via mild solvent evaporation method, and a polydopamine assisted surface coating of 1-dodecanethiol (DT) on a melamine sponge (MS) substrate. Surface structure and performance of MS/PDA/DT sponge were characterized by Scanning Electron Microscope (SEM), Fourier transform infrared spectrometer (FTIR) and Video Optical Contact Angle (CA) meter. The results showed that the as-prepared MS/PDA/DT sponge has a high water contact angle (WCA) of 147.2°, which is probably attributed to both the rough surface derived from in situ growth and the low surface energy due to grafting of hydrophobic 1-dodecanethiol. The durability of the as-constructed MS/PDA/DT sponge was studied by repeated abrasion tests. After 50 abrasion cycles, the superhydrophobicity of MS/PDA/DT sponge good mechanical durability. The MS/PDA/DT sponge can effectively absorb oil with an absorption capacity of up to 24 times its own weight. The superhydrophobic and superoleophilic MS/PDA/DT sponge has potential as a promising absorbent for oil/water separation.
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http://dx.doi.org/10.1080/09593330.2021.1942559DOI Listing
June 2021

16S rDNA Full-Length Assembly Sequencing Technology Analysis of Intestinal Microbiome in Polycystic Ovary Syndrome.

Front Cell Infect Microbiol 2021 10;11:634981. Epub 2021 May 10.

Center of Reproductive Medicine, Shengjing Hospital of China Medical University, Shenyang, China.

Objective: To study the characteristics and relationship of the gut microbiota in patients with polycystic ovary syndrome (PCOS).

Method: We recruited 45 patients with PCOS and 37 healthy women from the Reproductive Department of Shengjing Hospital. We recorded their clinical indexes, and sequenced their fecal samples by 16S rDNA full-length assembly sequencing technology (16S-FAST).

Result: We found decreased α diversity and different abundances of a series of microbial species in patients with PCOS compared to healthy controls. We found LH and AMH were significantly increased in PCOS with enterotype when compared to control women with enterotype, while glucose and lipid metabolism level remained no significant difference, and situations were opposite in PCOS and control women with enterotype. and were more abundant while spp. were less abundant in the PCOS group. was significantly more prevalent in PCOS-not insulin resistance (NIR) compared to control-NIR and PCOS-not overweight (NOW) patient groups compared to control-NOW groups. Kyoto Encyclopedia Genes and Genomes reflecting pathways related to lipopolysaccharide biosynthesis were more abundant in the PCOS group.

Conclusion: Our study found gut microbiota that had different abundance in patients with PCOS compared to healthy controls. An intimate relationship was shown between the gut microbiota and pathological changes in PCOS. We suggest the gut microbiota should be taken into consideration in the treatment of symptoms of PCOS drugs and diet.
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http://dx.doi.org/10.3389/fcimb.2021.634981DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141595PMC
May 2021

Characteristics and potential sources of wintertime air pollution in Linfen, China.

Environ Monit Assess 2021 Apr 8;193(5):252. Epub 2021 Apr 8.

Beijing Polytechnic, Beijing, 100176, China.

Linfen in China's Shanxi Province suffers severe air pollution in winter. Understanding the characteristics of air pollution and providing scientific support to mitigate such pollution are urgent matters. This study investigated the variations of PM, PM, NO, SO, O, and CO in Linfen between December 1, 2019 and February 29, 2020. The mean concentrations of PM, PM, NO, SO, MDA8 (the maximum daily 8-h average) O, and CO were 106.2, 139.4, 47.2, 41.0, 57.0 μg m, and 1.8 mg m, respectively. Large amounts of pollutants emitted by coal burning, industry, vehicles, and residents contributed to air pollution. Unfavorable meteorological conditions, such as lower temperature, weaker wind, higher relative humidity, and reduced planetary boundary layer height, made the situation worse. Fireworks and firecrackers set off to celebrate traditional Chinese festivals caused the concentration of PM pollutants to spike, with the maximum daily mean concentration of PM reached 314 μg m and the peak hourly value reached 378.0 μg m. Suspensions of commercial and social activities due to COVID-19 reduced anthropogenic emissions, mainly from industry and transportation, which decreased the level of air pollutants other than O. Analyses involving backward trajectory cluster, the potential source contribution function, and concentration weighted trajectory demonstrated that PM pollution mainly came from local emissions in Shanxi Province and regional transport from Inner Mongolia, Shaanxi, Hebei, Henan, and Gansu provinces. Shanxi and its surrounding provinces should adopt measures such as tightening environmental management standards, promoting the use of renewable energy, and adjusting the transportation structure to reduce regional emissions. This study will help policy-makers draft plans and policies to reduce air pollution in Linfen.
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http://dx.doi.org/10.1007/s10661-021-09036-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8031341PMC
April 2021

Association of exposure to ambient air pollution with ovarian reserve among women in Shanxi province of north China.

Environ Pollut 2021 Jun 3;278:116868. Epub 2021 Mar 3.

College of Environment and Resource, Research Center of Environment and Health, Shanxi University, Taiyuan, Shanxi, 030006, China. Electronic address:

Air pollution has been an important risk factor for female reproductive health. However, epidemiological evidence of ambient air pollution on the predictor for ovarian reserve (antral follicle count, AFC) is deficient. We aim to comprehensively evaluate the association of long-term exposure to ambient air pollution with AFC among women of reproductive age in Shanxi of north China. 600 women with spontaneous menstrual cycle, not using controlled ovarian stimulation, were enrolled in the retrospective study. Two distinct periods of antral follicle development were designed as exposure windows. Generalized linear model was employed to estimate the change of AFC associated with exposure of atmospheric pollutants (SO, NO, PM, PM, CO and O). Stratification analysis based on age (<30, ≥30 years), university degree (yes, no), years of exposure (2013-2016, 2017-2019) and duration of infertility (<2, 2-5, >5 years) along with two pollutants model were employed to further illustrate the association. We found every 10 μg/m increase in SO concentration level during the entire development stage of antral follicle was associated with a -0.01 change in AFC (95% confidence interval: -0.016, -0.002) adjusting for the confounders including age, BMI, parity and infertility diagnosis factors. The significant association of increased SO level with decreased AFC was particularly observed during the early transition from primary follicle to preantral follicle stage by 10 μg/m increase in SO exposure level with a -0.01 change (95% CI: -0.015, -0.002) in AFC. The negative association was pronounced among women aged ≥30 years old, and also significant in two pollutants model after adjusting the confounders. No significant associations between other air pollutants and AFC were observed. Our finding suggests that long-term exposure to air pollutant SO is associated with lower AFC, raising our concern that atmospheric SO exposure may have potential adverse impact on women ovarian reserve.
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http://dx.doi.org/10.1016/j.envpol.2021.116868DOI Listing
June 2021

The function of BAP18 on modulation of androgen receptor action in luteinized granulosa cells from normal weight women with and without PCOS.

Mol Cell Endocrinol 2021 05 1;527:111228. Epub 2021 Mar 1.

Center of Reproductive Medicine, Shengjing Hospital of China Medical University, Shenyang City, Liaoning Province, 110004, China. Electronic address:

Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in reproductive-age women. In this study, BPTF associated protein of 18 kDa (BAP18) is decreased in luteinized granulosa cells (GCs) from PCOS women. BAP18 depletion significantly decreases CYP19A1 expression levels, leading to an abrogation in transfer capacity of androgen to estrogen in GCs. Also, BAP18 knockdown delays cell cycle G1 to S phase transition and induces cell apoptosis to decrease GCs proliferation. We also provide evidence showing BAP18 interacts with androgen receptor (AR) and enhances AR-mediated transactivation in GCs. Results indicate that AR or BAP18 recruits to androgen response elements (AREs) of CYP19A1 and FSHR, which are putative AR-induced genes in GCs. BAP18 interacts with Sp1 transcription factor and co-recruits to the promoter region of AR gene, resulting in AR transactivation in GCs. Taken together, these data provide new insights on the pathophysiology of PCOS.
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http://dx.doi.org/10.1016/j.mce.2021.111228DOI Listing
May 2021

Nuclear DNA replicates during zygote development in Arabidopsis and Torenia fournieri.

Plant Physiol 2021 02;185(1):137-145

Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life Science, Northwest University, Xi'an 710069, China.

The progression of the cell cycle is continuous in most cells, but gametes (sperm and egg cells) exhibit an arrest of the cell cycle to await fertilization to form a zygote, which then continues through the subsequent phases to complete cell division. The phase in which gametes of flowering plants arrest has been a matter of debate, since different phases have been reported for the gametes of different species. In this study, we reassessed the phase of cell-cycle arrest in the gametes of two species, Arabidopsis (Arabidopsis thaliana) and Torenia fournieri. We first showed that 4', 6-diamidino-2-phenylindole staining was not feasible to detect changes in gametic nuclear DNA in T. fournieri. Next, using 5-ethynyl-2'-deoxyuridine (EdU) staining that detects DNA replication by labeling the EdU absorbed by deoxyribonucleic acid, we found that the replication of nuclear DNA did not occur during gamete development but during zygote development, revealing that the gametes of these species have a haploid nuclear DNA content before fertilization. We thus propose that gametes in the G1 phase participate in the fertilization event in Arabidopsis and T. fournieri.
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http://dx.doi.org/10.1093/plphys/kiaa014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133679PMC
February 2021

Inhibition of fibroblast IL-6 production by ACKR4 deletion alleviates cardiac remodeling after myocardial infarction.

Biochem Biophys Res Commun 2021 Apr 18;547:139-147. Epub 2021 Feb 18.

Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China; Key Laboratory of Biological Targeted Therapy of the Ministry of Education, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. Electronic address:

Fibrotic scarring is tightly linked to the development of heart failure in patients with post-myocardial infarction (MI). Atypical chemokine receptor 4 (ACKR4) can eliminate chemokines, such as C-C chemokine ligand 21 (CCL21), which is independently associated with heart failure mortality. However, the role of ACKR4 in the heart during MI is unrevealed. This study aimed to determine whether ACKR4 modulates cardiac remodeling following MI and to illuminate the potential molecular mechanisms. The expression of ACKR4 was upregulated in the border/infarct area, and ACKR4 was predominantly expressed in cardiac fibroblasts (CFs). Knockout of ACKR4 protected against adverse ventricular remodeling in mice post-MI. These protective effects of ACKR4 deficiency were independent of dendritic cell immune response but could be attributed to downregulated CF-derived IL-6, affecting CF proliferation and endothelial cell (EC) functions, which consequently inhibited cardiac fibrosis. ACKR4 promoted IL-6 generation and proliferation of CFs. Besides, ACKR4 induced endothelial-to-mesenchymal transition (EndMT) in ECs through IL-6 paracrine effect. The p38 MAPK/NF-κB signaling pathway was involved in ACKR4 facilitated IL-6 generation. Moreover, ACKR4 overexpression in vivo via AAV9 carrying a periostin promoter aggravated heart functional impairment post-MI, which was abolished by IL-6 neutralizing antibody. Therefore, our study established a novel link between ACKR4 and IL-6 post-MI, indicating that ACKR4 may be a novel therapeutic target to ameliorate cardiac remodeling.
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http://dx.doi.org/10.1016/j.bbrc.2021.02.013DOI Listing
April 2021

Impairments of cortico-cortical connectivity in fine tactile sensation after stroke.

J Neuroeng Rehabil 2021 02 15;18(1):34. Epub 2021 Feb 15.

Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong, China.

Background: Fine tactile sensation plays an important role in motor relearning after stroke. However, little is known about its dynamics in post-stroke recovery, principally due to a lack of effective evaluation on neural responses to fine tactile stimulation. This study investigated the post-stroke alteration of cortical connectivity and its functional structure in response to fine tactile stimulation via textile fabrics by electroencephalogram (EEG)-derived functional connectivity and graph theory analyses.

Method: Whole brain EEG was recorded from 64 scalp channels in 8 participants with chronic stroke and 8 unimpaired controls before and during the skin of the unilateral forearm contacted with a piece of cotton fabric. Functional connectivity (FC) was then estimated using EEG coherence. The fabric stimulation induced FC (SFC) was analyzed by a cluster-based permutation test for the FC in baseline and fabric stimulation. The functional structure of connectivity alteration in the brain was also investigated by assessing the multiscale topological properties of functional brain networks according to the graph theory.

Results: In the SFC distribution, an altered hemispheric lateralization (HL) (HL degree, 14%) was observed when stimulating the affected forearm in the stroke group, compared to stimulation of the unaffected forearm of the stroke group (HL degree, 53%) and those of the control group (HL degrees, 92% for the left and 69% for the dominant right limb). The involvement of additional brain regions, i.e., the distributed attention networks, was also observed when stimulating either limb of the stroke group compared with those of the control. Significantly increased (P < 0.05) global and local efficiencies were found when stimulating the affected forearm compared to the unaffected forearm. A significantly increased (P < 0.05) degree of inter-hemisphere FC (interdegree) mainly within ipsilesional somatosensory region and a significantly diminished degree of intra-hemisphere FC (intradegree) (P < 0.05) in ipsilesional primary somatosensory region were observed when stimulating the affected forearm, compared with the unaffected forearm.

Conclusions: The alteration of cortical connectivity in fine tactile sensation post-stroke was characterized by the compensation from the contralesional hemisphere and distributed attention networks related to involuntary attention. The interhemispheric connectivity could implement the compensation from the contralateral hemisphere to the ipsilesional somatosensory region. Stroke participants also exerted increased cortical activities in fine tactile sensation.
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http://dx.doi.org/10.1186/s12984-021-00821-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885375PMC
February 2021

Well-Defined, Versatile and Recyclable Half-Sandwich Nickelacarborane Catalyst for Selective Carbene-Transfer Reactions.

Chemistry 2021 Mar 3;27(18):5754-5760. Epub 2021 Mar 3.

Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi, 710054, P. R. China.

Catalytic carbene-transfer reactions constitute a class of highly useful transformations in organic synthesis. Although catalysts based on a range of transition-metals have been reported, the readily accessible nickel(II)-based complexes have been rarely used. Herein, an air-stable nickel(II)-carborane complex is reported as a well-defined, versatile and recyclable catalyst for selective carbene transfer reactions with low catalyst loading under mild conditions. This catalyst is effective for several types of reactions including diastereoselective cyclopropanation, epoxidation, selective X-H insertions (X = C, N, O, S, Si), particularly for the unprotected substrates. This represents a rare example of carborane ligands in base metal catalysis.
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http://dx.doi.org/10.1002/chem.202005014DOI Listing
March 2021

A new air-stable Si,S-chelating ligand for Ir-catalyzed directed C-H borylation.

Org Biomol Chem 2021 Jan;19(2):355-359

Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an, China. and Xi'an Key Laboratory of Sustainable Energy Materials Chemistry, Xi'an Jiaotong University, Xi'an, China.

A new air-stable Si,S-chelating ligand has been developed and used in an iridium-catalyzed ortho C-H borylation reaction with a broad substrate scope. This study provides the first example of using a sulfur-containing ligand in the catalytic C-H borylation process. It provides a rapid, efficient, and economical method for the preparation of organoboron compounds.
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http://dx.doi.org/10.1039/d0ob02335cDOI Listing
January 2021

[Cloning and expression analysis of CpPMM gene in Codonopsis pilosula].

Zhongguo Zhong Yao Za Zhi 2020 Sep;45(18):4382-4391

College of Pharmacy, Shanxi Medical University Jinzhong 030600, China.

GDP-mannose is an important precursor for the synthesis of Codonopsis pilosula polysaccharides and involved in the synthesis of sugar chains. Phosphomannomutase(PMM)catalyzes the conversion of mannose-6-phosphate(Man-6-P)to mannose-1-phosphate(Man-1-P)to synthesize GDP-mannose. In this study, specific primers were designed based on the PMM gene sequence information in transcriptome data, and the full length of the C. pilosula PMM gene was cloned and named CpPMM. The correlation between the CpPMM gene expression and C. pilosula polysaccharide synthesis was analyzed by a series of bioinformatics analysis, prokaryotic expression and qRT-PCR. The results show that the CpPMM gene contains a 741 bp open reading frame(ORF), encoding 246 amino acids, which is highly similar to the PMM of other species and highly homologous to the Helianthus annuus from the Asteraceae family. It was predicted to be a hydrophilic non-transmembrane protein without signal peptide, which was predicted to be located in the cytoplasm with multiple phosphorylation sites. Combined with predictive analysis of conserved domains, this protein belongs to the HAD(haloacid dehalogenase)superfamily; prokaryotic expression studies show that the size of the CpPMM fusion protein is about 29 kDa, which is consistent with the relative molecular mass predicted. The target protein is an inclusion body and is partially soluble. The qRT-PCR results showed that the CpPMM gene exerted spatiotemporal expression patterns, and the expression level in fruiting period was significantly higher than that in the other three periods such as the flowering period. Along with the growth period of C. pilosula, the polysaccharide content of C. pilosula showed a gradual increase trend, reaching the highest during the harvest time. And there are significant differences in the polysaccharide content of C. pilosula in each period. In this study, the CpPMM gene was cloned from the root of C. pilosula, at the same time, the prokaryotic expression system was constructed. In addition, its gene expression level is highly correlated with the polysaccharide content of C. pilosula. It lays the foundation for further studying the function of CpPMM gene and the analysis of biosynthetic pathways of polysaccharides in medicinal plants.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200626.101DOI Listing
September 2020

The Contribution of PEG Molecular Weights in PEGylated Emulsions to the Various Phases in the Accelerated Blood Clearance (ABC) Phenomenon in Rats.

AAPS PharmSciTech 2020 Nov 2;21(8):300. Epub 2020 Nov 2.

College of Pharmacy, Shenyang Pharmaceutical University, No.103 Wenhua Road, Shenyang, 110016, Liaoning, China.

PEGylated preparations will be cleared rapidly from blood circulation when they are administrated twice in the same animal at a time interval, referred to as the "accelerated blood clearance" (ABC) phenomenon. Commonly, the study of the ABC phenomenon was investigated in two aspects: induction phase and effectuation phase. Herein, we report the influence of physicochemical properties (PEG molecular weights) in the induction phase and effectuation phase on the ABC phenomenon. In the experiment, on one hand, PEGylated emulsions with different molecular weights of PEG (refer to PEn, n = 400, 600, 800, 1000, 2000, and 5000) were injected for the first dose (induction phase) and induced PE2000 to produce ABC phenomenon. On the other hand, after PE2000 injected, PEn was injected for the second dose (effectuation phase). The results indicated that PE2000 and PE5000 induced an intense ABC phenomenon by their long-circulating characteristic. Interestingly, PE400, PE600, PE800, and PE1000 produced a consistent ABC phenomenon but different circulation time. Apparently, the induction of the ABC phenomenon is not only determined by the circulation time but also by the PEG molecular weights. When PEn is in the effectuation phase, the extent of the ABC phenomenon was not positively related to the molecular weights of PEG, increasing first and then weaken with the increase of molecular weights of PEG. These suggest that the number of -(CHCHO)- repeat units of PE2000 was more conducive to interact with anti-PEG IgM. The results reported here clearly indicate that both the PEG molecular weights of prior dose and the subsequent dose of emulsion strongly influence the extent of the ABC phenomenon. Taken together, our observations in this study complete the effect of PEG molecular weights at a different phase of the ABC phenomenon. Furthermore, our findings have a significant impact on the choice of molecular weights for PEGylated formulations for use in cross-administration.
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http://dx.doi.org/10.1208/s12249-020-01838-2DOI Listing
November 2020

Neuro-perceptive discrimination on fabric tactile stimulation by Electroencephalographic (EEG) spectra.

PLoS One 2020 28;15(10):e0241378. Epub 2020 Oct 28.

Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hong Kong, China.

The precise evaluation of sensory perceptions during fabric-skin interactions is still poorly understood in neuroscience. This study aims to investigate the cortical sensory response to fabric stimuli with different textiles by Electroencephalographic (EEG) spectral intensities, and evaluate the relationships between EEG frequency bands, traditional subjective questionnaires, and the materials' physical properties. Twelve healthy adult participants were recruited to test three fabrics with different textile compositions of 1) cotton, 2) nylon, and 3) polyester and wool. The physical properties of the fabrics were quantitatively evaluated by a Fabric Touch Tester (FTT). Subjects were invited to rate the sensory perception of the fabric samples via a subjective questionnaire and objective EEG recording. Significant differences in the EEG relative spectral power of Theta and Gamma bands were acquired in response to the different fabric stimuli (P<0.05). The Theta and Gamma powers demonstrated a significant correlation with the most of the subjective sensations evaluated by questionnaire and the fabrics' physical properties by FTT (P<0.05). The EEG spectral analysis could feasibly be used for the discrimination of fabric stimuli with different textile compositions and further indicates sensory perceptions during fabric stimulation. This finding may provide evidence for further exploratory research of sensory perceptions via EEG spectral analysis, which could be applied to the study of brain generators of skin tactility in future prostheses and the automatic detection of sensory perception in industries.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0241378PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7592732PMC
December 2020

Multi-Step Continuous-Flow Organic Synthesis: Opportunities and Challenges.

Chemistry 2021 Mar 21;27(15):4817-4838. Epub 2021 Jan 21.

Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, P. R. China.

Continuous-flow multi-step synthesis takes the advantages of microchannel flow chemistry and may transform the conventional multi-step organic synthesis by using integrated synthetic systems. To realize the goal, however, innovative chemical methods and techniques are urgently required to meet the significant remaining challenges. In the past few years, by using green reactions, telescoped chemical design, and/or novel in-line separation techniques, major and rapid advancement has been made in this direction. This minireview summarizes the most recent reports (2017-2020) on continuous-flow synthesis of functional molecules. Notably, several complex active pharmaceutical ingredients (APIs) have been prepared by the continuous-flow approach. Key technologies to the successes and remaining challenges are discussed. These results exemplified the feasibility of using modern continuous-flow chemistry for complex synthetic targets, and bode well for the future development of integrated, automated artificial synthetic systems.
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http://dx.doi.org/10.1002/chem.202004477DOI Listing
March 2021

Effects of descending or ascending stair exercise on body composition, insulin sensitivity, and inflammatory markers in young Chinese women with obesity: A randomized controlled trial.

J Sports Sci 2021 Mar 4;39(5):496-502. Epub 2020 Oct 4.

Physical Education College, Hebei Normal University, Shijiazhuang, China.

We examined the effects of descending (DSE) or ascending (ASE) stair exercise on body composition, insulin sensitivity, and inflammatory markers in young Chinese women with obesity. Thirty-six participants were randomly assigned into three groups DSE, ASE and a control group. The DSE and ASE groups performed three sessions of stair walking per week for 12 weeks with a gradual increase in repetitions. Following the exercise interventions, body composition related variables obtained by Dual-energy X-ray absorptiometry scans significantly decreased. Abdominal fat decreased in the DSE group only. Moreover, Insulin sensitivity improved significantly 3.5-fold in the DSE group compared with ASE group (insulin: -33.2% vs. -9.8%, homoeostasis model assessment for insulin resistance: -35.6% vs. -10.8%). Pro-inflammatory factors showed significant decreases in tumour necrosis factor-α (TNF-α) (-39.9% vs. -23.2%) for both intervention groups. The reduction in TNF-α concentrations in the DSE group was significantly different compared to the other two groups. Interleukin-6 significantly decreased in both exercise protocols. Our results show that 12-weeks induced stair walking improved body composition parameters in Chinese females with obesity. The results also demonstrate the superiority of the DSE protocol for improving insulin sensitivity. These findings may be attributable to the decreases observed in TNF- α levels.
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http://dx.doi.org/10.1080/02640414.2020.1829362DOI Listing
March 2021

A Unique Population of Regulatory T Cells in Heart Potentiates Cardiac Protection From Myocardial Infarction.

Circulation 2020 Nov 28;142(20):1956-1973. Epub 2020 Sep 28.

Department of Cardiology, Union Hospital, and Key Laboratory of Biological Targeted Therapy of the Ministry of Education (N.X., Y. Lu, M.G., N.L., M.L., J.J., Z.Z., J.L., D.L., T.T., B.L., S.N., M.Z., M.L., Y. Liao, X.C.), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Regulatory T cells (Tregs), traditionally recognized as potent suppressors of immune response, are increasingly attracting attention because of a second major function: residing in parenchymal tissues and maintaining local homeostasis. However, the existence, unique phenotype, and function of so-called tissue Tregs in the heart remain unclear.

Methods: In mouse models of myocardial infarction (MI), myocardial ischemia/reperfusion injury, or cardiac cryoinjury, the dynamic accumulation of Tregs in the injured myocardium was monitored. The bulk RNA sequencing was performed to analyze the transcriptomic characteristics of Tregs from the injured myocardium after MI or ischemia/reperfusion injury. Photoconversion, parabiosis, single-cell T-cell receptor sequencing, and adoptive transfer were applied to determine the source of heart Tregs. The involvement of the interleukin-33/suppression of tumorigenicity 2 axis and Sparc (secreted acidic cysteine-rich glycoprotein), a molecule upregulated in heart Tregs, was further evaluated in functional assays.

Results: We showed that Tregs were highly enriched in the myocardium of MI, ischemia/reperfusion injury, and cryoinjury mice. Transcriptomic data revealed that Tregs isolated from the injured hearts had plenty of differentially expressed transcripts in comparison with their lymphoid counterparts, including heart-draining lymphoid nodes, with a phenotype of promoting infarct repair, indicating a unique characteristic. The heart Tregs were accumulated mainly because of recruitment from the circulating Treg pool, whereas local proliferation also contributed to their expansion. Moreover, a remarkable case of repeatedly detected T-cell receptor of heart Tregs, more than that of spleen Tregs, suggests a model of clonal expansion. Besides, HeliosNrp-1 phenotype proved the mainly thymic origin of heart Tregs, with a small contribution of phenotypic conversion of conventional CD4 T cells, proved by the analysis of T-cell receptor repertoires and conventional CD4 T cells adoptive transfer experiments. The interleukin-33/suppression of tumorigenicity 2 axis was essential for sustaining heart Treg populations. Last, we demonstrated that Sparc, which was highly expressed by heart Tregs, acted as a critical factor to protect the heart against MI by increasing collagen content and boosting maturation in the infarct zone.

Conclusions: We identified and characterized a phenotypically and functionally unique population of heart Tregs that may lay the foundation to harness Tregs for cardioprotection in MI and other cardiac diseases.
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http://dx.doi.org/10.1161/CIRCULATIONAHA.120.046789DOI Listing
November 2020

Do We Need to Distinguish Thrombolysis and Nonthrombolysis Patients When Applying Stroke-Associated Pneumonia Predicting Scores? An External Validation from a 2-Center Database.

Med Sci Monit 2020 Sep 14;26:e924129. Epub 2020 Sep 14.

Medical School of Southeast University, Nanjing, Jiangsu, China (mainland).

BACKGROUND Due to the lack of validation for predictive scoring of stroke-associated pneumonia in both thrombolysis- and nonthrombolysis-treated ischemic stroke (IS) patients, this study aimed to evaluate 4 scoring methods in the 2 subgroups. MATERIAL AND METHODS The CerebroVascular Database Project database included data from patients with cerebral IS that were admitted in 2 hospitals from February 2016 to January 2018. A total of 138 thrombolysis-treated and 138 nonthrombolysis-treated IS patients were enrolled. Area under receiver operating characteristic curves (AUROC) were performed to examine the discrimination of the 4 scores, and Hosmer-Lemeshow test was used to evaluate the goodness of fit. RESULTS The incidence of stroke-associated pneumonia was 24.8%. The thrombolysis and nonthrombolysis subgroups were not significantly different with regard to sex, present smoking, chronic obstructive pulmonary disease history, atrial fibrillation history, blood pressure, or glucose level on admission. However, significant differences were found in National Institutes of Health Stroke Scale scores (P<0.001), Glascow Coma Scale scores (P<0.001), Oxfordshire Community Stroke Project classification (P<0.001), dysphagia (P<0.001), and white blood cell counts (P=0.039). The AUROC for the Age, Atrial fibrillation, Dysphagia, male Sex, stroke Severity, National Institutes of Health Stroke Scale; Preventive ANtibacterial THERapy in acute Ischemic Stroke; Acute Ischemic Stroke-Associated Pneumonia Score (AIS-APS); and Independence, Sex, Age, National Institutes of Health Stroke Scale scores in total population were 0.80 (0.74-0.84), 0.75 (0.69-0.80), 0.80 (0.76-0.85), and 0.76 (0.71-0.81). The goodness of fit was 0.22, 0.22, 0.27, and 0.17, respectively. The AUROC of 4 scores between subgroups were not statistically significant. CONCLUSIONS The AIS-APS had the highest AUC and goodness of fit in our population. All 4 scores can be applied regardless of whether thrombolysis has been performed on patients.
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http://dx.doi.org/10.12659/MSM.924129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7513614PMC
September 2020

Ergonomic mastectomy bra design: Effect on core body temperature and thermal comfort performance.

Appl Ergon 2021 Jan 7;90:103249. Epub 2020 Sep 7.

Dr Stephen Hui Research Centre for Physical Recreation and Wellness, Hong Kong Baptist University, Hong Kong, China. Electronic address:

The accumulation of sweat and heat between the skin and the prosthesis is one of the common causes of discomfort experienced by post-mastectomy women who wore silicone-type external breast prostheses. This study aimed 1) to investigate the effect of a newly designed heat-reduction mastectomy bra on the thermal responses and thermal comfort performance; and 2) to propose an evaluation protocol for post-mastectomy products. The heat-reduction bra and the conventional bra were made of the same textile materials but the cup of the bra under discussion had a polyurethane cup with a perforated structure along the breast root/inframammary fold. The inframammary fold is often aggravated by heat, sweat, maceration, chafe, and lack of air circulation. Nine healthy male participants were recruited to participate in the study since the women who had undergone double-mastectomy were sensitive about their scars. An ingestible telemetric pill sensor was used to collect the data of core body temperature. Participants performed a 70-min five-phase exercise protocol wearing the heat-reduction bra and the conventional bra together with a silicone prosthesis with ventilation holes on the bottom. Physiological responses (i.e., core body temperature, skin temperature, temperature between bra and prosthesis, skin humidity and humidity between bra and prosthesis) and subjective sensation responses were measured. The results were statistically significant and the conventional mastectomy bra showed a higher core body temperature than that of the heat-reduction mastectomy bra (37.3 ± 0.3 °C to 38.2 ± 0.5 °C vs. 37.2 ± 0.3 °C to 38.0 ± 0.3 °C) starting from the middle of the sitting phase to the running phase and post-exercise resting (F = 164.2, p < 0.001). Both factors, the bra and the phase, have significant effects on the core body temperature (F = 14.5, p < 0.001). The heat-reduction mastectomy bra demonstrated optimum thermal comfort performance in both the temperature and humidity than the conventional mastectomy bra.
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http://dx.doi.org/10.1016/j.apergo.2020.103249DOI Listing
January 2021

Experimental study of optical hyperbolic metamaterials for high-efficiency spatial filtering.

Opt Lett 2020 Sep;45(17):4923-4926

We experimentally study the optical properties of Al- hyperbolic metamaterials. The hyperbolic dispersion and ultrathin Al film ∼12 make the Al- multilayers possess transmission efficiency ∼0.3 for the spatial frequency ranging from to 1.42 at 363.8 nm illumination. The atomic concentration of 3% Cu-doping is experimentally demonstrated to obtain Al film ∼12 with root-mean-square roughness ∼0.49. The fabricated Al- multilayers combined with the optimized resonant grating with period 280 nm serves as a structured illumination device, which efficiently converts the -polarized normal field to the spatial frequency 1.3 structured field. The measured average transmission intensity of the ±1 order is ∼0.14, and the intensity ratio of the ±1 order and 0 order is ∼65. This Letter is promising for structured illumination, spontaneous emission enhancement, Cherenkov radiation, etc.
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http://dx.doi.org/10.1364/OL.402049DOI Listing
September 2020

Integrated study of metabolomics and gut metabolic activity from ulcerative colitis to colorectal cancer: The combined action of disordered gut microbiota and linoleic acid metabolic pathway might fuel cancer.

J Chromatogr A 2020 Oct 20;1629:461503. Epub 2020 Aug 20.

School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China. Electronic address:

Colorectal cancer (CRC) is one of the most serious complications of ulcerative colitis (UC). Altered gut microbiota is implicated in the development of CRC and metabolic perturbations are often associated with changes in the gut microbiome composition. Given the links between gut microbiome and the metabolic profiles in the body, an approach involving ultra-high-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) metabolomics and 16S rDNA sequencing technology was applied to trace the development UC into CRC in rats. The study identified 11 differential metabolites related to both UC and CRC, which mainly referred to the linoleic acid metabolism. Among these, linoleic acid and 12‑hydroxy‑8,10-octadecadienoic acid could serve as key biomarkers for the development of UC into CRC. Besides, a significant change was observed in the microflora structure during the development from UC to CRC; this mainly involved a gradual increase in Escherichia-Shigella and a gradual decrease in Lactobacillus. In addition, Pearson's correlation analysis revealed strong correlations between intestinal microflora-related metabolites and specific intestinal microflora, which indicated both of them can promote the transition of UC to CRC. The results of the present study provided positive support for the involvement of intestinal microflora and host metabolism in the pathophysiological mechanism that is responsible for the development of UC into CRC. This information can help understand the risk for CRC that accompanies a diagnosis of UC and also provide different means of targeting these differential metabolites and intestinal microbiota to avoid UC-induced CRC.
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http://dx.doi.org/10.1016/j.chroma.2020.461503DOI Listing
October 2020

Simultaneous quantification of eleven bioactive phenolic compounds in pigeon pea natural resources and in vitro cultures by ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-QqQ-MS/MS).

Food Chem 2021 Jan 25;335:127602. Epub 2020 Jul 25.

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, PR China.

Bioactive phenolics primarily contribute to versatile health benefits of pigeon pea. For the first time, an UPLC-QqQ-MS/MS method was developed for the quantitative analysis of eleven bioactive phenolic compounds in pigeon pea natural resources (seeds, leaves, and roots) and in vitro cultures (calli and hairy roots). The proposed method could be achieved within 6 min of running time, and displayed the satisfactory linearity, sensitivity, precision, accuracy, and stability. According to analytical results, the distribution of eleven target compounds in different organs of pigeon pea was clarified. Also, it was surprisingly found that pigeon pea in vitro cultures exhibited superiority in contents of genistin and cajaninstilbene acid as compared with natural resources. Overall, the present work provided a rapid and sensitive analysis approach, which could be useful not only for quality control of pigeon pea natural resources, but also for applicability and safety evaluation of pigeon pea in vitro cultures.
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http://dx.doi.org/10.1016/j.foodchem.2020.127602DOI Listing
January 2021

Effective Production of Phenolic Compounds with Health Benefits in Pigeon Pea [ (L.) Millsp.] Hairy Root Cultures.

J Agric Food Chem 2020 Aug 28;68(31):8350-8361. Epub 2020 Jul 28.

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, Heilongjiang 150040, People's Republic of China.

Phenolic compounds in pigeon pea possess various biological properties beneficial to human health. In this study, pigeon pea hairy root cultures (PPHRCs) were developed as an effective platform for the production of phenolic compounds. A high-productive hairy root line was screened and characterized, and its culture conditions were optimized in terms of biomass productivity and phenolic yield. The comparative profiling of 10 phenolic compounds in PPHRCs and pigeon pea natural resources (seeds, leaves, and roots) was achieved by ultra-high-performance liquid chromatography-tandem mass spectrometry analysis. The total phenolic yield in PPHRCs (3278.44 μg/g) was much higher than those in seeds (68.86 μg/g) and roots (846.03 μg/g), and comparable to leaves (3379.49 μg/g). Notably, PPHRCs exhibited superiority in the yield of the most important health-promoting compound cajaninstilbene acid (2996.23 μg/g) against natural resources (4.42-2293.31 μg/g). Overall, PPHRCs could serve as promising potential alternative sources for the production of phenolic compounds with nutraceutical/medicinal values.
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http://dx.doi.org/10.1021/acs.jafc.0c02600DOI Listing
August 2020

Simultaneous determination of taxoids and flavonoids in twigs and leaves of three Taxus species by UHPLC-MS/MS.

J Pharm Biomed Anal 2020 Sep 2;189:113456. Epub 2020 Jul 2.

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, 150040, PR China.

Taxus species are highly concerned due to the presence of anticancer taxoids (especially paclitaxel) and health beneficial flavonoids. For the first time, an UHPLC-MS/MS method was developed for the simultaneous determination of seven taxoids and seven flavonoids in twigs and leaves of three Taxus species. The satisfactory separation of fourteen target compounds was achieved within 5 min of running time on an Agilent ZORBAX Eclipse Plus C18 column (50 mm × 2.1 mm I.D., 1.8 μm) using an acetonitrile-water gradient elution program. Mass transitions of all analytes in selected reaction monitoring acquisition mode were systematically optimized for obtaining the highest signal intensities. Regression equations of all analytes exhibited excellent linearities with coefficients higher than 0.9990, and the lowest limits of quantification of all analytes ranged from 0.01 to 1.66 ng/mL. The intra- and inter-day precisions (relative standard deviations) of all analytes were less than 4.17% for retention time and less than 7.42% for peak area, and the spiking standard recoveries of all analytes ranged from 96.85%-104.77%. By the aid of the proposed method, the distribution of fourteen target compounds in twigs and leaves of Taxus chinensis, Taxus cuspidata, and Taxus media was clearly figured out. Overall, the present work provided a rapid and valid UHPLC-MS/MS approach, which could not only be useful for quality control and applicability assessment of twigs and leaves of the three Taxus species in pharmaceutical and nutraceutical industries, but also offer a good reference for the systematic analysis of taxoids and flavonoids in other Taxus species.
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http://dx.doi.org/10.1016/j.jpba.2020.113456DOI Listing
September 2020

Measurement of sensory deficiency in fine touch after stroke during textile fabric stimulation by electroencephalography (EEG).

J Neural Eng 2020 07 24;17(4):045007. Epub 2020 Jul 24.

Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong, People's Republic of China.

Objective: Sensory deficiency of fine touch limits the restoration of motor functions after stroke, and its evaluation was seldom investigated from a neurological perspective. In this study, we investigated the cortical response measured by electroencephalography (EEG) on the fine touch sensory impairment during textile fabric stimulation after stroke.

Approach: Both participants with chronic stroke (n = 12, stroke group) and those unimpaired (n = 15, control group) were recruited. To investigate fine touch during textile fabric stimulations, full brain EEG recordings (64-channel) were used, as well as the touch sensation questionnaires based on the American Association of Textile Chemists and Colorists (AATCC) Evaluation Procedure 5. During the EEG measurement, relative spectral power (RSP) and EEG topography were used to evaluate the neural responses toward the fabric stimuli. In the subjective questionnaire, the fine touch for fabric stimuli was rated and represented by 13 different sensation parameters. The correlation between the fine touch evaluated by the EEG and the questionnaire was also investigated.

Main Results: The neural responses of individuals with fine touch impairments after stroke were characterized by a shifted power spectrum to a higher frequency band, enlarged sensory cortical areas and higher RSP intensity (P < 0.05). Asymmetric neural responses were obtained when stimulating different upper limbs for both unimpaired participants and stroke participants (P < 0.05). The fine touch sensation of the stroke participants was impaired even in the unaffected limb. However, as a result of different neural processes, the correlation between the EEG and the questionnaire was weak (r < 0.2).

Significance: EEG RSP was able to capture the varied cortical responses induced by textile fabric fine touch stimulations related to the fine touch sensory impairment after stroke.
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http://dx.doi.org/10.1088/1741-2552/aba160DOI Listing
July 2020

[Correlation of the Expression Alteration of Tim-3 with the T Cell and B Cell Dysfunction in Peripheral Blood of Multiple Myeloma Patients].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2020 Jun;28(3):881-885

Department of Hematology, The First Affiliated Hospital of Xi'an Medical College, Xi'an 710021, Shaanxi Province, China.

Objective: To study the correlation of the expression alteration of Tim-3 with the T cell and B cell dysfunction in peripheral blood of multiple myeloma (MM) patients.

Methods: 30 patients diagnosed as MM from October 2016 to October 2018 were selected and enrolled in MM group, and 30 healthy persons whose sex and age was matched with the MM patients were selected and enrolled in healthy control group (HC). The blood samples from MM patients and HC were collected, and the peripheral blood mononuclear cells (PBMNC) were separated by density gradient centrifugation, then the serum was kept for further study. The ratios of CD3CD4Tim-3T cells, CD3CD8Tim-3T cells and the CD19+CD20-CD38+B cells were analysed by flow cytometry (FCM),and the concentration of T cell-related cytokines IFN-γ, TNF-αand B cell-related antibodies IgA, IgM and IgG were measured by ELISA. At the same time, the differences of the ratios of CCD3CD4Tim-3T, CD3CD8Tim-3T cells and plasmablast and the concentration of IFN-γ, TNF-α, IgA, IgM and IgG between the MM patient and HC were estimated, and the correlation of the ratio of CD3CD4Tim-3T, CD3CD8Tim-3T cells with the ratio of plasmablast and the concentration of IFN-γ, TNF-α, IgA, IgM and IgG in MM patients were analyzed.

Results: The ratio of CD3CD4Tim-3T, CD3CD8Tim-3T cells increased in MM patients, while the ratio of CD19+CD20- CD38+B cells and the concentration of IFN-γ, TNF-α, IgA, IgM and IgG decreased in MM patients. And there was a negative correlation of the ratio of CD3CD4Tim-3T cells with CD19+CD20-CD38+B cells and the concentration of IFN-γ, IgA, IgM and IgG in MM patients, while the ratio of CD3CD8Tim-3T cells just negatively correlated with the concentration of TNF-α.

Conclusion: Expression of Tim-3 on CD4 and CD8 cells elevates in the peripheral blood of MM patients, which also correlates with the function suppression of T and B cells.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2020.03.027DOI Listing
June 2020

Recent Progress in Continuous-Flow Hydrogenation.

ChemSusChem 2020 Jun 20;13(11):2876-2893. Epub 2020 May 20.

Frontier Institute of Science and Technology (FIST), Xi'an Jiaotong University, Xi'an, 710049, P.R. China.

To achieve a safe, efficient, and sustainable (even fully automated) production for the continuous-flow hydrogenation reactions, which is among the most often used reactions in chemical synthesis, new catalyst types and immobilization methods as well as flow reactors and technologies have been developed over the last years; in addition, these approaches have been combined with new and transformational technologies in other fields such as artificial intelligence. Thus, attention from academic and industry practitioners has increasingly focused on improving the performance of hydrogenation in flow mode by reducing the reaction times, increasing selectivities, and achieve safe operation. This Minireview aims to summarize the most recent research results on this topic with focus on the advantages, current limitations, and future directions of flow chemistry.
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http://dx.doi.org/10.1002/cssc.202000778DOI Listing
June 2020

The Long Noncoding RNA Hotair Regulates Oxidative Stress and Cardiac Myocyte Apoptosis during Ischemia-Reperfusion Injury.

Oxid Med Cell Longev 2020 12;2020:1645249. Epub 2020 Mar 12.

Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Oxidative stress and subsequent cardiac myocyte apoptosis play central roles in the initiation and progression of myocardial ischemia-reperfusion (I/R) injury. Homeobox transcript antisense intergenic RNA () was previously implicated in various heart diseases, yet its role in myocardial I/R injury has not been clearly demonstrated. Mice with cardiac-restricted knockdown or overexpression of were exposed to I/R surgery. H9c2 cells were cultured and subjected to hypoxia/reoxygenation (H/R) stimulation to further verify the role and underlying mechanisms of in vitro. Histological examination, molecular detection, and functional parameters were determined in vivo and in vitro. In response to I/R or H/R treatment, expression was increased in a bromodomain-containing protein 4-dependent manner. Cardiac-restricted knockdown of exacerbated, whereas overexpression prevented I/R-induced oxidative stress, cardiac myocyte apoptosis, and cardiac dysfunction. Mechanistically, we observed that exerted its beneficial effects via activating AMP-activated protein kinase alpha (AMPK). Further detection revealed that activated AMPK through regulating the enhancer of zeste homolog 2/microRNA-451/calcium-binding protein 39 (EZH2//Cab39) axis. We provide the evidence that endogenous lncRNA is an essential negative regulator for oxidative stress and cardiac myocyte apoptosis in myocardial I/R injury, which is dependent on AMPK activation via the EZH2//Cab39 axis.
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http://dx.doi.org/10.1155/2020/1645249DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7091551PMC
December 2020