Publications by authors named "Jianwen Li"

88 Publications

Identification of hub genes associated with neutrophils infiltration in colorectal cancer.

J Cell Mol Med 2021 Apr 5;25(7):3371-3380. Epub 2021 Mar 5.

Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Colorectal cancer (CRC) is the leading cause of cancer-related mortality in the world. Accumulating evidence indicate that tumour infiltrating immune cells participated in cancer progression. Among them, tumour infiltrating neutrophils (TINs) are reported to play crucial role in various cancers. In this study, we used CIBERSORTx, a digital cytometry tool to evaluate the neutrophils infiltration in CRC based on gene expression data of CRC tissues from GSE39582 data set and The Cancer Genome Atlas data set (TCGA-COAD and TCGA-READ). Weighted gene co-expression network analysis (WGCNA) was conducted in GSE39582 data set to identify hub genes associated with neutrophil infiltration. The association of hub gene and neutrophils was then validated in TCGA cohorts and an independent RJ cohort. Functional analysis was performed to investigate the molecular mechanisms of the interested hub gene. We found that neutrophil infiltration is elevated in CRC tissues, and it is related to a poorer prognosis. A total of 18 gene modules are identified by WGCNA in GSE39582 data set, among which lightcyan module is significantly correlated with neutrophils infiltration. Furthermore, Superoxide Dismutase 2 (SOD2) in lightcyan module was proved to correlated with neutrophils infiltration in various cancer types. In addition, SOD2 expression is highly associated with several chemokines, including CXCL8, a neutrophils-related attractant, and functional analysis revealed that SOD2 is involved in neutrophils recruitment biological process. These results indicate that an 'SOD2-CXCL8-neutrophil recruitment' axis plays a potential role in colorectal cancer progression.
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http://dx.doi.org/10.1111/jcmm.16414DOI Listing
April 2021

MDM2/4 amplification predicts poor response to immune checkpoint inhibitors: a pan-cancer analysis.

ESMO Open 2020 30;5(1):e000614. Epub 2020 Sep 30.

Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, China; State Key Laboratory of Oncology in South China, Guangzhou, China; Collaborative Innovation Center for Cancer Medicine, Guangzhou, China. Electronic address:

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http://dx.doi.org/10.1136/esmoopen-2019-000614DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046406PMC
September 2020

Synergistic Approach to Develop Antibacterial Electrospun Scaffolds Using Honey and -Nitroso--acetyl Penicillamine.

ACS Biomater Sci Eng 2021 02 4;7(2):517-526. Epub 2021 Jan 4.

School of Chemical, Materials and Biomedical Engineering, University of Georgia, Athens 30602, Georgia, United States.

Bacterial infections have been increasingly recognized as the major reason for the failure of tissue engineering scaffolds. Therefore, there is a need for novel and multifunctional biomaterials that not only enhance tissue regeneration but also can combat infections. An antibacterial and bioactive scaffold was fabricated in this study by incorporation of honey and a nitric oxide (NO) donor, -nitroso--acetyl-penicillamine (SNAP), into polylactic acid (PLA) nanofibers using a single-jet electrospinning method. The morphology of the prepared nanofibers was observed using a scanning electron microscope. PLA/honey/SNAP (PLA/HN/SNAP) nanofibers had an average diameter of 624.92 ± 137.69 nm and showed a sustained release of NO for 48 h. The scaffolds were characterized for their chemical composition via Fourier-transform infrared spectroscopy. Moreover, the tensile properties of nanofibers along with their wettability, water retention ability, and water vapor transmission rate were evaluated. The results of antibacterial studies revealed that the synergistic combination of honey and SNAP significantly reduced the viability of Gram positive and Gram negative . In addition, qualitative and quantitative 3T3 fibroblast cell culturing experiments proved that the PLA/HN/SNAP scaffolds supported better cell attachment and proliferation compared to PLA. The promising results obtained in this study indicate that PLA/HN/SNAP nanofibrous scaffolds have great potential for tissue engineering applications.
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http://dx.doi.org/10.1021/acsbiomaterials.0c01411DOI Listing
February 2021

Impact of STAT1 polymorphisms on crizotinib-induced hepatotoxicity in ALK-positive non-small cell lung cancer patients.

J Cancer Res Clin Oncol 2021 Mar 2;147(3):725-737. Epub 2021 Jan 2.

Department of Medical Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, Guangzhou, 510060, People's Republic of China.

Purpose: Crizotinib is the first-line small molecule tyrosine kinase inhibitor for ALK-positive non-small cell lung cancer. In this study, a retrospective pharmacogenomics investigation was conducted to explore the relationship between genes related to RTK downstream signaling pathways and crizotinib-induced hepatic toxicity in ALK-positive NSCLC patients.

Methods: The variable importance analysis of random forest algorithm was applied to identify the significant features which contribute to the crizotinib sensitivity in Cancer Cell Line Encyclopedia (CCLE) database. The KEGG and reactome pathway enrichment analysis were conducted with EnrichR. The differential expression genes were identified with R package DESeq2 in CCLE liver derived cell lines between crizotinib sensitive and resistant groups. From 2012 to 2015, 42 NSCLC patients were enrolled in this study. 90 polymorphisms were genotyped using the Sequenom Massarray system. Sequencing of HGFR (c-Met) genes was carried out on the Ion Torrent Proton.

Results: In total, 66.7% NSCLC patients suffered from crizotinib-induced liver toxicity and 11.9% progressed to severe hepatic toxicity. The features with the top importance from classification and regression random forest model were enriched in RTK downstream signaling pathways (JAK/STAT, RAS/RAF/MAPK, PI3K/AKT pathways) and immune system-related pathways. Collagen family genes (COL1A1, COL1A2, COL6A1, COL5A1) and other extracellular matrix protein (TNC, TAGLN, TENM2, EDIL3, VCAN, CNN1, SH3BP4, TAGLN), which were closely related to MAPK-ERK signaling pathways, were significantly enriched in crizotinib resistant cell lines. In multiple logistic regression, STAT1 rs10208033 (T > C) was significantly associated with crizotinib-induced liver toxicity (OR = 6.733, 95% CI 1.406-32.24, P = 0.017). Compared with non-CC, OR is 5.5 (95% CI 1.219-24.81, P = 0.027) for STAT1 rs10208033 CC genotype to develop crizotinib-induced liver toxicity. Further cell viability test in human fetal hepatocyte line, L-02, reveals that the STAT1 inhibitor might protect hepatocyte cells from the toxicity caused by crizotinib.

Conclusion: Polymorphism of rs10208033 is a potential biomarker for predicting crizotinib-induced hepatotoxicity. These results suggest that STAT1 plays an important role in crizotinib-induced hepatotoxicity. Further studies are needed to confirm our finding and understand the underlying mechanisms.
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http://dx.doi.org/10.1007/s00432-020-03476-4DOI Listing
March 2021

Effects of Occupational Radiation Exposure on Job Stress and Job Burnout of Medical Staff in Xinjiang, China: A Cross-Sectional Study.

Med Sci Monit 2020 Dec 24;26:e927848. Epub 2020 Dec 24.

Department of Occupational and Environmental Health, College of Public Health, Xinjiang Medical University, Urumqi, Xinjiang, China (mainland).

BACKGROUND Although the potential effects of long-term and low-dose radiation exposure on physical health have attracted considerable attention, few systematic evaluations have been reported regarding the mental health of occupational groups. This study sought to investigate the effects of occupational radiation exposure on job stress and job burnout of medical radiation staff. MATERIAL AND METHODS Using cluster random sampling, a total of 1573 medical radiation workers were initially selected from 10 hospitals in Xinjiang, China, and 1396 valid questionnaires were finally collected. Job stress and job burnout were assessed using the Effort-Reward Imbalance (ERI) questionnaire and the Chinese Maslach Burnout Inventory (CMBI), respectively. RESULTS The percentages of medical radiation staff experiencing job stress and job burnout were 53.08% and 63.32%, respectively. A statistically significant difference in job stress was observed in association with age, ethnicity, professional title, marital status, radiation work type, radiation working years, family history, hypertension, obesity, smoking, and drinking (P<0.05). A statistically significant difference in job burnout was observed in association with age, sex, ethnicity, professional title, educational level, marital status, job post, radiation work type, radiation working years, family history, hypertension, diabetes, and obesity (P<0.05). Female (odds ratio [OR]=0.75, 95% confidence interval [CI]: 0.58-0.98), senior professional title (OR=0.64, 95% CI: 0.43-0.96), and radiation work types of nuclear medicine (OR=0.15, 95% CI: 0.07-0.33) and radiotherapy (OR=0.54, 95% CI: 0.36-0.79) were protective factors, and job stress (OR=4.57, 95% CI: 3.55-5.91) was the risk factor for job burnout of medical radiation staff. CONCLUSIONS Medical radiation staff experience high levels of job stress and job burnout. The interventions of occupational physical examination, personal dose monitoring, occupational health education, and management optimization are recommended to relieve job stress and job burnout and enhance occupational health of medical radiation staff.
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http://dx.doi.org/10.12659/MSM.927848DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7771202PMC
December 2020

Development and application of a comparative risk assessment method for ranking chemical hazards in food.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2021 Jan 4;38(1):1-14. Epub 2020 Dec 4.

NHC Key Laboratory of Food Safety Risk Assessment, Chinese Academy of Medical Science Research Unit (2019RU014), China National Center for Food Safety Risk Assessment , Beijing, China.

The aim of this study was to develop a comparative risk assessment method to prioritise the public health risks posed by chemical hazards in food. Through a literature review, and in light of expert opinions, a bottom-up, semi-quantitative scoring method was applied to screen the ranking metrics and assign a score. In addition, a metrics system and a ranking model were constructed. The fuzzy comprehensive analysis model was used to assess typical chemical hazards in a specific food, as well as to rank risks in many foods. Data were collected from the National Food Surveillance System in China, the Food Consumption of Chinese Residents Database, government reports, public websites and databases of authoritative organisations. The comparative risk assessment method was applied to case studies on ranking chemical hazards in different kinds of food. According to application testing, the method truly reflects the overall risk and ranking of chemical hazards in food.
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http://dx.doi.org/10.1080/19440049.2020.1828627DOI Listing
January 2021

Estimation of Peanut Leaf Area Index from Unmanned Aerial Vehicle Multispectral Images.

Sensors (Basel) 2020 Nov 25;20(23). Epub 2020 Nov 25.

College of Agriculture, South China Agricultural University, Guangzhou 510642, China.

Leaf area index (LAI) is used to predict crop yield, and unmanned aerial vehicles (UAVs) provide new ways to monitor LAI. In this study, we used a fixed-wing UAV with multispectral cameras for remote sensing monitoring. We conducted field experiments with two peanut varieties at different planting densities to estimate LAI from multispectral images and establish a high-precision LAI prediction model. We used eight vegetation indices (VIs) and developed simple regression and artificial neural network (BPN) models for LAI and spectral VIs. The empirical model was calibrated to estimate peanut LAI, and the best model was selected from the coefficient of determination and root mean square error. The red (660 nm) and near-infrared (790 nm) bands effectively predicted peanut LAI, and LAI increased with planting density. The predictive accuracy of the multiple regression model was higher than that of the single linear regression models, and the correlations between Modified Red-Edge Simple Ratio Index (MSR), Ratio Vegetation Index (RVI), Normalized Difference Vegetation Index (NDVI), and LAI were higher than the other indices. The combined VI BPN model was more accurate than the single VI BPN model, and the BPN model accuracy was higher. Planting density affects peanut LAI, and reflectance-based vegetation indices can help predict LAI.
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http://dx.doi.org/10.3390/s20236732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728055PMC
November 2020

Dietary exposure of general Chinese population to fatty acid esters of 3-monochloropropane-1, 2-diol (3-MCPD) from edible oils and oil-containing foods.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2021 Jan 24;38(1):60-69. Epub 2020 Nov 24.

NHC Key Laboratory of Food Safety Risk Assessment, Chinese Academy of Medical Science Research Unit (2019RU014), China National Center for Food Safety Risk Assessment , Beijing, China.

Edible oils and oil-containing foods have been regarded as the main source of 3-monochloropropane-1, 2-diol (3-MCPD) esters. A total of 3,847 individual food samples were collected in China to carry out 3-MCPD fatty acid esters analysis. The samples comprising edible oils, fried foods and bakery foods from 31 Chinese provinces, municipalities and autonomous regions were obtained from the national food contaminant information system during 2015-2017. The dietary exposure and potential risks associated with the consumption of 3-MPCD esters from edible oils and oil-containing foods were estimated using a semi-probabilistic assessment model. Concentrations of 3-MCPD fatty acid esters in food samples were detected by gas chromatography-mass spectrometry (GC-MS). The mean levels of 3-MCPD fatty acid esters in edible oils, fried foods and bakery foods were 0.862, 0.249 and 0.145 mg/kg, respectively. The range of mean dietary intakes of 3-MCPD esters in different subpopulation groups (classified by gender and age) was from 0.586 to 1.539 μg/kg bw/day, which were all lower than 2 μg/kg bw/day - the tolerable daily intake (TDI) established by the European Food Safety Authority (EFSA). The range of dietary intake of 3-MCPD esters in high consumers (95th percentile) in each group was from 1.511 to 4.027 μg/kg bw/day, which accounted for 75.6% to 201.4% of the TDI. The 3-MCPD esters exposure level of 3.5% of the total Chinese population exceeded the TDI. The findings indicate that the potential health risks caused by dietary 3-MCPD esters from edible oils and oil-containing foods were of low concern for most of the Chinese population. However, the exposure risk of the consumers with excessive edible oil consumption calls for attention.
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http://dx.doi.org/10.1080/19440049.2020.1834151DOI Listing
January 2021

Dietary exposure to mineral oil hydrocarbons of Chinese infants aged 0-6 months.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2020 Dec 5;37(12):2070-2081. Epub 2020 Nov 5.

Department of Toxicology, Tianjin Centers for Disease Control and Prevention , Tianjin, China.

Consumers are exposed to a range of mineral oil hydrocarbons (MOH) via food. The potential adverse health effect of MOH varies widely. Since infant formula (IF) is the major food source for infants, it is necessary to understand MOH exposure and consequent health impact. In the present study, occurrence data of 42 IF samples and food consumption data of 0-6 months infants from China National Food Consumption Survey in 2015 were linked to evaluate the dietary exposure to MOH of 0-6 months infants in China. Ordinary consumers (who purchased IF for 0-6 months infants in different packaging type randomly), packaging type loyal- and brand loyal-consumers were selected as three representative populations. For ordinary consumers and packaging-loyal consumers, dietary exposure to MOH was estimated both deterministically and probabilistically. For brand-loyal consumers, point-estimation was used as the exposure assessment method. Due to toxicological gaps for MOHs, it was inappropriate to derive health-based guidance value and perform the robust human health risk assessment. MOE approach was used to characterise MOSH risk. The no-observed-adverse-effect level for induction of liver microgranulomas, 19 mg/kg BW per day, was used as a reference point for calculating margins of exposure (MOEs) for MOSH exposure. Although first exposure occurs in babies, there are no relevant toxicology studies. All MOE values for different scenarios were higher than 100. There are no dose-response data on the carcinogenicity of MOAH mixtures and hence it is not possible to establish a reference point to calculate the MOE and characterise its risk. Therefore, it is not possible to draw conclusions about the full nature of possible concerns for infants aged 0-6 months. This study evaluates by a probabilistic approach the dietary intake of Chinese infants aged 0-6 months to MOH for the first time and describes the associated uncertainties.
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http://dx.doi.org/10.1080/19440049.2020.1828625DOI Listing
December 2020

Reconstruction of the chest wall in locally advanced breast cancer with multi-disciplinary cooperation: a case report of mesh repair plus TRAM combined with DIEP chest wall reconstruction.

Gland Surg 2020 Aug;9(4):1048-1055

Department of Breast Surgery, Oncology, Chest Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.

Locally advanced breast cancer, which is defined as a malignant breast tumor that invades or adheres to the surrounding tissue, is characterized by the invasion of the chest wall and the skin surface by the tumor. Multiple lymph nodes are invaded and fuse into a mass, causing extensive axillary lymph node metastasis. However, locally advanced breast cancer does not exhibit distant metastasis. At present, in most hospitals in China and the rest of the world, this type of breast cancer is primarily managed through systematic and local treatments. However, a consensus concerning the optimal surgical method for chest wall reconstruction, which for many surgeons is a difficult and confusing procedure, has not been reached. In the past, many breast centers had used skin flap combined with hard mesh titanium alloy plate to repair the large chest wall defects. Although titanium alloy plate can maintain the stability of the chest wall, it may have a negative effect on the follow-up radiotherapy of breast cancer patients, which is a controversial method. In addition, titanium alloy mesh also has the risk of deformation and fracture. These factors will cause some hidden dangers to patient safety. According to the research, the soft mesh not only has the characteristics of satisfactory compatibility and robustness for maintaining the stability of chest wall, but also does not affect the postoperative radiotherapy of patients. Combined with the advantages of soft mesh, Our department treated a case of locally advanced breast cancer with chest wall invasion. Through cooperation between the breast surgery and thoracic surgery departments, a mesh repair plus transverse rectus abdominis myocutaneous (TRAM) combined with deep inferior epigastric perforator (DIEP) procedure was performed to remove the breast tumor and repair the large area of skin defect after surgery, and a relatively satisfactory therapeutic effect was achieved. In this case, we took two novel approaches: first, a 4-layer high-density polyethylene mesh was used to repair the defect; secondly, the inferior epigastric artery perforation was anastomosed with the thoracoacromial artery (end-to-end anastomosis) and the inferior epigastric vein perforation was anastomosed with the axillary vein (end-to-side anastomosis).
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http://dx.doi.org/10.21037/gs-20-436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475378PMC
August 2020

The Optimal Timing of Hydrogel Injection for Treatment of Intervertebral Disc Degeneration: Quantitative Analysis Based on T1ρ MR Imaging.

Spine (Phila Pa 1976) 2020 Nov;45(22):E1451-E1459

Department of Orthopaedics, the Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, China.

Study Design: Animal experimental study.

Objective: The aim of this study was to investigate the optimal time of hydrogel injection for regenerating intervertebral disc degeneration (IDD) based on T1ρ magnetic resonance imaging (MRI).

Summary Of Background Data: Currently, different approaches are being pursued to regenerate the IDD. However, the optimal timing for the regenerative intervention is unclear.

Methods: The slowly, progressive IDD models were established in 18 rhesus monkeys. On the basis of the MR T1ρ values of the discs, the rhesus monkeys were divided into severe (T1ρ values: <81 ms), moderate (T1ρ values: 81∼95 ms), and mild (T1ρ values: 96∼110 ms) degeneration groups. Biocompatible hydrogel was injected into the central part of the nucleus pulposus of the discs under fluoroscopic guidance. Treatment effects were investigated using radiography, T1ρ MRI, and histology until 12 months postoperatively.

Results: After injection, the T1ρ values of all the discs increased significantly at 1 month postoperatively, and then remained at approximately 110 ms in the mild and moderate groups during the whole observation period, with no significant difference compared to the values at 1 month (P > 0.05). However, in the severe group, the T1ρ values decreased significantly after 1 month and leveled at approximately 70 ms after 6 months, with significant difference compared to the values at 1 month (P < 0.05). In the mild and moderate groups, there were no significant differences between preoperative histological scores and those at 12 months (P > 0.05). However, the histological score in the severe group at 12 months was significantly higher than the preoperative scores (P < 0.05).

Conclusion: This study suggested that the moderate degenerative stage of IDD (T1ρ values from 95 to 80 ms) could be the optimal time for hydrogel injection aimed at the regenerative intervention, based on T1ρ-MR imaging technique and quantitative analysis.

Level Of Evidence: N/A.
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http://dx.doi.org/10.1097/BRS.0000000000003667DOI Listing
November 2020

Poisonous delicacy: Market-oriented surveys of the consumption of Rhododendron flowers in Yunnan, China.

J Ethnopharmacol 2021 Jan 28;265:113320. Epub 2020 Aug 28.

Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China; Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences, Yezin, Nay Pyi Taw, 05282, Myanmar. Electronic address:

Ethnopharmacological Relevance: Plants from the family Ericaceae, and in particular those in the genus Rhododendron are frequently reported to contain grayanotoxins. Plant products such as honey and herbal medicines made from these plants occasionally contain grayanotoxins, and in turn may lead to intoxication. The balance between the benefits and risk of poisoning from Rhododendrons is of concerns. This study explores the ethnobotanical knowledge of the people in Yunnan, China as regards the consumption of Rhododendron flowers, and gives special focus to their assessment of the benefit-risk balance.

Materials And Methods: An ethnobotanical survey was conducted across 14 county-level local markets in north and central Yunnan province, during which a total of 82 stalls selling Rhododendron flowers were visited and 204 people were interviewed. Voucher specimens were obtained under the guidance of collectors, and details about local practices and knowledge were recorded using semi-structured interviews and participatory observations.

Results: The consumption of the corollas of Rhododendron decorum Franch. Flowers (RDf) or Rhododendron pachypodum Balf. f. & W.W. Sm. Flowers (RPf) as a seasonal delicacy is a long-standing tradition in the study area. RDf are widely consumed in northwest and northeast Yunnan, while RPf are more prevalent in the central regions of Yunnan, and there is a high consistency in the knowledge of the process for detoxification or palatability for each species. The main reasons for eating the flowers were listed as health benefits (mostly clear heat), wild collected, tradition and good flavor. All RPf consumers stated that the corolla from this species is not toxic, while 67.4% of the RDf consumers claim that the corolla from RDf is toxic. We compared the two species and analyzed their process intensities, poisoning cases and cautions, market trade forms and existing toxicity studies, which agreed well and consistently that the corolla of RDf deserve more toxicity attention than RPf.

Conclusion: Our study provides a window to look into the ways, beyond honey and herbal medicine, by which Rhododendron species have influenced human wellbeing. The local culture can justify eating Rhododendron flowers, and meanwhile, has developed a series of skills to avoid the side effects of eating them, and therefore the study also provides a good case to answer more general questions about the rationality of eating any plant products by assessing the trade-off between benefits and side effects.
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http://dx.doi.org/10.1016/j.jep.2020.113320DOI Listing
January 2021

Current prevalence of perioperative early venous thromboembolism and risk factors in Chinese adult patients with inguinal hernia (CHAT-1).

Sci Rep 2020 07 29;10(1):12667. Epub 2020 Jul 29.

Beijing Institute of Respiratory Diseases, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100043, China.

Venous thromboembolism (VTE) is an important postoperative complication. We investigated and analyzed the current inguinal hernia treatment methods and occurrence of early postoperative VTE in Chinese adults. This study involved data for patients with inguinal hernia hospitalized in 58 general hospitals in mainland China from January 1st, 2017 to December 31st, 2017. Data were retrospectively analyzed using a questionnaire. After data inputting and cleaning, we stratified and statistically analyzed patients' data using Caprini scores to create a high-, middle-, and low-risk group. A total of 14,322 patients with inguinal hernia were admitted to the 58 participating hospitals. After data collation and cleaning, 13,886 patients (97.0%) met our inclusion and exclusion criteria. The percentages of laparoscopic surgery and open surgery were 51.2% and 48.8%, respectively. 16 VTEs occurred during the hospitalization, accounting for 0.1% of all adverse events (95% confidence interval (CI) 0.11-0.13). The incidence of VTE was 0.2% (95% CI 0.18-0.2) in the high-risk group and 0.02% (95% CI 0.01-0.03) in the middle-risk group, based on Caprini scoring, with a significant difference (p < 0.0001). No VTE occurred in the low-risk group. Only 3,250 (23.4%) patients underwent Caprini risk assessment regarding treatment, with 13.2% receiving any prevention and only 1.2% receiving appropriate prevention. The treatment of inguinal hernia in Chinese adults has progressed somewhat; however, the evaluation and prevention of perioperative VTE was seriously neglected, in our study, and the incidence of postoperative VTE was underestimated postoperatively. Risk factors continue to be inadequately considered.
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http://dx.doi.org/10.1038/s41598-020-69453-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391649PMC
July 2020

Sleeve gastrectomy ameliorates alveolar structures and surfactant protein expression in lungs of obese and diabetic rats.

Int J Obes (Lond) 2020 12 27;44(12):2394-2404. Epub 2020 Jul 27.

Department of General Surgery, The First Affiliated Hospital of Shandong First Medical University, Jinan, Shandong Province, 250014, People's Republic of China.

Background: Bariatric surgeries have been shown to be effective in reversing damaged pulmonary function in individuals suffering from obesity and type 2 diabetes mellitus, whereas its underlying mechanisms remain largely unknown.

Methods: Sleeve gastrectomy (SG) was performed on obese and diabetic Wistar rats, and their pulmonary function and lung tissues were compared to sham-operated (SH) obese and diabetic rats, and age-matched healthy controls (C) to explore the improvements in microstructures and expression of surfactant protein (SP)-A and -C at postoperative 4th, 8th, and 12th week.

Result: Apart from the profound metabolic changes and improvement in pulmonary function, lung volume was restored along with an improved diffusion capacity noted by thinned capillary basement membrane and decreased harmonic mean length of diffusion barrier in SG rats. The digital slices of light microscope showed the general changes brought on by the SG, including normalized basic structures, ameliorated inflammatory status, as well as reduced lipid deposition, where the hydroxyproline (HYP), triglyceride (TG) assays, and electron microscope further suggested that the improvement in alveolar structures lies in reduced collagen fibers, lipids and septal tissues, increased capillary blood, and normalized alveolar type 2 (AT2) cells. Besides, disrupted SP-A and SP-C expression were also normalized after SG.

Conclusion: The improvement of lung function after SG is related to the ameliorated alveolar structures, and surface protein expression induced by weight loss and improved glucose metabolism.
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http://dx.doi.org/10.1038/s41366-020-0647-yDOI Listing
December 2020

Bioinformatics analysis integrating metabolomics of mA RNA microarray in intervertebral disc degeneration.

Epigenomics 2020 08 6;12(16):1419-1441. Epub 2020 Jul 6.

Department of Orthopedics, The Seventh Affiliated Hospital of Sun Yat-sen University, No. 628, Zhenyuan Rd, Shenzhen, 518107, China.

To explore the potential functions and mechanism of .methyladenosine (mA) abnormality of RNAs in nucleus pulposus from the intervertebral disc degeneration (IDD). We performed rat model, mA epitranscriptomic microarray, bioinformatics analysis and metabolomics. In IDD, most of the differentially methylated RNAs showed a significant demethylation situation. The competing endogenous RNA network // combining downstream Wnt pathway were identified in bioinformatics analysis. For metabolomics, activation of Wnt pathway led to reprogramming of glucose metabolism and enzyme activation of . Finally, quantitative real-time PCR and methylated RNA immunoprecipitation coupled with quantitative real-time PCR revealed the positive correlation between demethylation of and expression of both and . might be demethylated by ZFP217, activating FTO and ///Wnt played a crucial role in IDD.
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http://dx.doi.org/10.2217/epi-2020-0101DOI Listing
August 2020

Modified radical mastectomy for anterior thoracic nerve and intercostobrachial nerve protection (case report).

Gland Surg 2020 Apr;9(2):463-466

Department of Breast Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.

Modified breast cancer radical mastectomy is a more common operating method in breast surgery. Traditional modified radical mastectomy focuses on protecting the long thoracic nerve and thoracodorsal nerve while ignoring the protection of the anterior thoracic nerve and intercostobrachial nerve protection, which leads often to patients with upper medial arm numbness, acid swelling, pain, chest atrophy, and other problems. In the modified radical mastectomy of breast cancer, in this case, the author used an elaborative operation to protect the anterior thoracic nerve and intercostobrachial nerve and thoroughly dissected the third-level lymph nodes through the axillary approach.
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http://dx.doi.org/10.21037/gs.2020.02.17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225455PMC
April 2020

Sleeve Gastrectomy Attenuates Diabetic Nephropathy by Upregulating Nephrin Expressions in Diabetic Obese Rats.

Obes Surg 2020 08;30(8):2893-2904

Department of General Surgery, The First Affiliated Hospital of Shandong First Medical University, 16766#, Jingshi Road, Jinan, 250014, Shandong Province, China.

Purpose: Diabetic nephropathy (DN) is the leading cause of end-stage renal disease, and sleeve gastrectomy (SG) is considered to be an effective strategy to improve pre-existing DN. However, the mechanism remains unknown.

Materials And Methods: Animal model of DN was induced by high-fat diet (HFD) and streptozotocin (STZ). SG or sham surgery was performed and rats were sacrificed at 4, 8, and 12 weeks after surgery. The basic parameters (blood glucose, body weight, kidney weight), indicators of renal function including serum creatinine (Scr), blood urea nitrogen (BUN), urine microalbumin, urine creatinine (Ucr), microalbumin creatinine ratio (UACR), ultrastructural changes of glomerulus, and the expression of nephrin gene and protein in glomerular podocytes were compared among groups.

Results: Blood glucose and body weight of SG rats were significantly lower than those of the sham-operated rats, and renal function of SG groups were also significantly improved within the postoperative period of 12 weeks. The results of periodic acid-Schiff staining (PAS) and transmission electron microscopy (TEM) showed that glomerular hypertrophy and accumulation of extracellular matrix proteins were significantly alleviated after SG, and the thickness of basement membrane and the fusion or effacement of foot processes were also significantly improved. The mRNA and protein expression of nephrin in SG groups was significantly higher than that in the sham group.

Conclusion: These results suggest that SG attenuates DN by upregulating the expression of nephrin and improving the ultrastructure of glomerular filtration membrane. This study indicates that SG can be used as an available therapeutic intervention for DN.
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http://dx.doi.org/10.1007/s11695-020-04611-3DOI Listing
August 2020

Trans fatty acid intake among Chinese population: a longitudinal study from 1991 to 2011.

Lipids Health Dis 2020 Apr 27;19(1):80. Epub 2020 Apr 27.

Department of Nutrition Division III, China National Center for Food Safety Risk Assessment, Beijing, 100022, P. R. China.

Objective: This study was aimed to roughly describe individual Trans Fatty Acids (TFAs) intake and the percentage of energy intake(E%), and identify major food sources in the Chinese population, taking gender, age, and regional distribution into the consideration, as well as examining temporal changes over the course of 20 years.

Method: This multi-center study, covering nine provinces among populations aged ≥ 3 in China, was conducted to collect food consumption information from 1991 to 2011. A classical assessment method was used to estimate the level of dietary TFA intake.

Results: Over the 20-year period, the intake of TFAs in Chinese populations had increased, but remained at a relatively lower level (from 0.25 g/d(0.11% for E%) to 0.53 g/d(0.24% for E%)) compared with that of other countries and the World Health Organization (WHO) recommended level. Collectively, males and participants aged 19-60 generally consumed more TFA-containing foods. People in eastern regions consumed more TFAs and had a higher E% than those in western area. Industrial sources of TFAs, especially vegetable oil, ranked as the principal food sources of TFAs in the Chinese population. Natural sources of TFAs have gradually increased in proportion among children and adolescents.

Conclusions: TFA intake and the E% are commonly under the recommended level in the general population in China. Presently, restriction of vegetable oil could be a crucial method to reduce TFA intake. It would be critical to facilitate and promote public health that food recommendations might be based on the dietary preferences for population separated by different ages and regions.
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http://dx.doi.org/10.1186/s12944-020-01247-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184713PMC
April 2020

Non-Vitamin K Antagonist Oral Anticoagulants Versus Warfarin in Patients with Atrial Fibrillation with Coronary or Peripheral Artery Disease.

Int Heart J 2020 Mar 14;61(2):231-238. Epub 2020 Mar 14.

Department of Cardiovascular Medicine, Xiangdong Hospital Hunan normal University.

The efficacy and safety of non-vitamin K antagonist oral anticoagulants (NOACs) in atrial fibrillation (AF) with coronary or peripheral artery disease (CAD or PAD) remain largely unresolved. We, therefore, conducted a meta-analysis to explore the effect of NOACs compared with warfarin in these populations.We systematically searched the Cochrane Library, PubMed, and Embase databases for randomized controlled trials (RCTs) involving NOACs versus warfarin in AF patients with CAD or PAD. A random-effect model was selected to pool the risk ratios (RRs) and 95% confidence intervals (CIs).A total of 7 RCTs were included. In AF patients with CAD, compared with warfarin use, the use of NOACs was associated with reduced risks of stroke/systemic embolism (RR 0.82; 95% CI 0.70-0.96) and intracranial hemorrhage (RR 0.41; 95% CI 0.26-0.63), but NOACs versus warfarin showed similar risks of all-cause death (RR 0.95; 95% CI 0.86-1.05), cardiovascular death (RR 0.95; 95% CI 0.80-1.13), stroke (RR 0.80; 95% CI 0.64-1.00), myocardial infarction (RR 1.00; 95% CI 0.83-1.21), and major bleeding (RR 0.82; 95% CI 0.65-1.04). Among patients with AF and PAD, NOACs versus warfarin had similar risks for stroke (RR 0.93; 95% CI 0.61-1.42), myocardial infarction (RR 1.10; 95% CI 0.64-1.90), all-cause death (RR 0.91; 95% CI 0.70-1.19), major bleeding (RR 1.12; 95% CI 0.70-1.81), and intracranial hemorrhage (RR 0.54; 95% CI 0.16-1.85).NOACs seem to be at least as effective and safe as warfarin in AF patients with CAD. whereas NOACs versus warfarin have similar efficacy and safety in patients with PAD.
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http://dx.doi.org/10.1536/ihj.19-202DOI Listing
March 2020

Pelvic peritoneum closure reduces postoperative complications of laparoscopic abdominoperineal resection: 6-year experience in single center.

Surg Endosc 2021 Jan 21;35(1):406-414. Epub 2020 Feb 21.

Department of General Surgery, Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Ruijin Er Road, Shanghai, 200025, China.

Background: To investigate feasibility of laparoscopic abdominoperineal resection with pelvic peritoneum closure (LAPR-PPC) for lower rectal cancer.

Methods: LAPR-PPC has been used for lower rectal cancer in our institution since 2014. In this study, we retrospectively analyzed the data from 86 patients who underwent LAPR-PPC and compared with the data from 96 patients who underwent laparoscopic APR without PPC (LAPR) from January 2013 to December 2018.

Results: The rate of perineal surgical site infection (SSI) (18.75% (18/96) vs. 5.81% (5/86), p < 0.01), delayed (> 4 weeks) perineal healing (12.50% (12/96) vs. 3.49% (3/86), p = 0.027), ileus (7.29% (7/96) vs 1.16% (1/86), p = 0.044), and postoperative perineal hernia (PPH, 5.21% (5/96) vs. 0% (0/86), p = 0.032) were significantly lower in LAPR-PPC group than LAPR group. The patients in LAPR-PPC group had shorter hospitalization time (21.32 ± 11.95 days vs. 13.93 ± 11.51 days, p < 0.01).

Conclusions: PPC procedure enabled the reduction in perineal wound complications, ileus, PPH, and consequently shortened hospitalization time. LAPR-PPC is beneficial for the patients with lower rectal cancer.
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http://dx.doi.org/10.1007/s00464-020-07414-wDOI Listing
January 2021

Chest CT screening in patients with overweight or obesity using spectral shaping at 150 kVp: compared with 120 kVp protocol and spectral shaping at 100 kVp protocol.

Jpn J Radiol 2020 May 11;38(5):451-457. Epub 2020 Feb 11.

Department of Radiology, The Second People's Hospital of Shizuishan, NO.246 West Youyi Street, Shizuishan, 753000, Ningxia, China.

Purpose: To evaluate the image quality (IQ) and the figure of merit (FoM) of chest CT screening in patients with overweight or obesity using a tin filter for spectral shaping at 150 kVp.

Materials And Methods: Patients with overweight or obesity (N = 150, body mass index ≥ 26 kg/m) with indications for chest CT screening were prospectively enrolled and randomly divided into three groups: 120 kVp group (standard radiation dose/tube voltage, 120 kVp/CT volume does index, 4.68 mGy); Sn100 kVp group (1/10th radiation dose level/100 kVp with a tin filter/0.47 mGy); Sn150 kVp group (1/2th radiation dose level/150 kVp with a tin filter/2.34 mGy). IQ and FoMs were evaluated and compared among the three groups.

Results: Image noise, signal-to-noise ratios and subjective IQ scores were significantly higher in the Sn150 kVp group than those in the Sn100 kVp group (all p < 0.05), but were not significantly different with those in the 120 kVp group. FoMs in the Sn150 kVp group were significantly higher than those in the 120 kVp group (all p < 0.05), but showed no statistical difference with those in the Sn100 kVp group.

Conclusions: Compared with scanning at 120 kVp, chest CT screening performed at 150 kVp with spectral shaping substantially reduces the radiation dose in overweight and obese patients while maintaining IQ.
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http://dx.doi.org/10.1007/s11604-020-00925-5DOI Listing
May 2020

Theoretical prediction of T-graphene as a promising alkali-ion battery anode offering ultrahigh capacity.

Phys Chem Chem Phys 2020 Feb 23;22(6):3281-3289. Epub 2020 Jan 23.

Department of Physics, Laboratory of Computational Materials Physics, Jiangxi Normal University, Nanchang 330022, China.

The pursuit of high capacity is one of the key challenges for the development of alkaline ion batteries (AIBs, namely Li/Na/K-ion batteries; LIBs, NIBs, or KIBs). Carbon-based anode materials represented by graphite have been widely used in secondary batteries; however, their storage capacity is generally not high. Graphene was once considered a promising candidate, but it has proven to be incapable of interacting strongly with alkali ions. Here, by first-principles calculations, we predict an allotrope of graphene that may soon be experimentally synthesized, called T-graphene, which is a promising anode material for AIBs. We find that when it is used as the anode for NIBs, its theoretical capacity can be as high as 2232 mA h g (NaC), which is six times that of graphite. For LIB and KIB anodes, the specific capacities are 744 mA h g and 1116 mA h g, corresponding to the LiC and KC chemical formula, respectively. We first demonstrate that the material is mechanically stable. We further show that the material has good electrical conductivity whether it is before or after adsorption of Li(Na or K). We also studied the diffusion of Li(Na or K) on its surface and found that their corresponding diffusion barriers are very low (Li, Na and K are about 0.37 eV, 0.35 eV and 0.25 eV, respectively), which means good rate performance. It is calculated that the average open circuit voltage of the corresponding three half-cells at full charge is also low (LIBs is about 0.20 V, NIBs is about 0.12 V, and KIBs are about 0.37 V), which is beneficial for increasing the operating voltage of the full battery. In addition, during the adsorption of lithium, sodium and potassium, the lattice change of the material is very small (about 1.0% for lithium, about 1.4% for sodium, and about 1.9% for potassium), which means good cycling performance. These results indicate that T-graphene is expected to replace graphene and become a very attractive anode material for AIBs.
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http://dx.doi.org/10.1039/c9cp06099eDOI Listing
February 2020

Vinpocetine inhibits RANKL-induced osteoclastogenesis and attenuates ovariectomy-induced bone loss.

Biomed Pharmacother 2020 Mar 15;123:109769. Epub 2019 Dec 15.

Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China. Electronic address:

Osteoporosis is a result of impaired bone formation and/or excessive bone resorption. Osteoclasts are the only cells in the body that have a bone resorption function. Inhibiting osteoclast activity and differentiation is a way to treat osteoporosis. The current pharmacological treatment for osteoporosis has many shortcomings, and more effective treatments are needed. Vinpocetine (Vinp), a derivative of the alkaloid vincamine, has been used to treat cerebrovascular disorders and cognitive impairment for a long time. Vinp inhibits mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB)-dependent inflammatory responses and oxidative damage in which osteoclasts are often involved. However, the effects of Vinp on the regulation of osteoclast activity remain unknown. In this study, we found that Vinp significantly inhibited receptor activator of NF-κB ligand (RANKL)-induced osteoclast and F-actin formation and decreased osteoclastic bone resorption in vitro. Vinp also suppressed the expression of osteoclast-specific genes, including NFATc1, c-Fos, tartrate-resistant acid phosphatase (TRAP), matrix metalloproteinase-9 (MMP-9), and cathepsin K (CTSK) at both the mRNA and protein levels. Vinp reduced activation of NF-κB, MAPK, and AKT signaling during osteoclastogenesis and prevented the production of reactive oxygen species with increased nuclear factor erythroid 2-related factor 2, heme oxygenase 1, and NAD(P)H:quinone acceptor oxidoreductase 1 expression. Animal experiments consistently demonstrated that Vinp treatment significantly attenuated ovariectomy-induced bone loss with a decrease in the osteoclast number and decreases in serum levels of RANKL, TRAP, interleukin-1β, and tumor necrosis factor-alpha, as well as increased serum levels of osteoprotegerin. Taken together, our findings reveal that Vinp may be a potential pharmacological choice for preventing and treating osteoporosis.
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http://dx.doi.org/10.1016/j.biopha.2019.109769DOI Listing
March 2020

Upregulated PTPN2 induced by inflammatory response or oxidative stress stimulates the progression of thyroid cancer.

Biochem Biophys Res Commun 2020 01 15;522(1):21-25. Epub 2019 Nov 15.

Department of Thyroid Breast Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524023, China. Electronic address:

PTPN2 is one of the members of the protein Tyrosine Phosphatases (PTPs) family. To explore the promotive effect of upregulated PTPN2 induced by inflammatory response or oxidative stress on the progression of thyroid cancer. PTPN2 level in thyroid cancer tissues and cell lines was detected. Kaplan-Meier method was applied for evaluating the prognostic value of PTPN2 in thyroid cancer patients. After stimulation of inflammatory response (treatment of IFN-γ and TNF-α), or oxidative stress (treatment of HO), protein level of PTPN2 in K1 cells was measured by Western blot. Regulatory effects of PTPN2 on EdU-positive staining and Ki-67 positive cell ratio in K1 cells either with HO stimulation or not were determined. PTPN2 was upregulated in thyroid cancer tissues and cell lines. Its level was higher in metastatic thyroid cancer patients than those of non-metastatic ones. High level of PTPN2 predicted worse prognosis of thyroid cancer. Treatment of either IFN-γ or TNF-α upregulated protein level of PTPN2 in K1 cells. Meanwhile, HO stimulation upregulated PTPN2, which was reversed by NAC administration. With the stimulation of increased doses of HO, EdU-positive staining and Ki-67 positive cell ratio were dose-dependently elevated. Silence of PTPN2 attenuated proliferative ability and Ki-67 expression in K1 cells either with HO stimulation or not. Inflammatory response or oxidative stress induces upregulation of PTPN2, thus promoting the progression of thyroid cancer.
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http://dx.doi.org/10.1016/j.bbrc.2019.11.047DOI Listing
January 2020

MicroRNA‑125a‑5p controls the proliferation, apoptosis, migration and PTEN/MEK1/2/ERK1/2 signaling pathway in MCF‑7 breast cancer cells.

Mol Med Rep 2019 Nov 24;20(5):4507-4514. Epub 2019 Sep 24.

Department of Vascular Thyroid Breast Surgery, Institute of Neurology, The Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China.

MicroRNA (miR)‑125a‑5p has shown the potential for suppressing tumorigenesis and development; however, the effects of miR‑125a‑5p on breast cancer cells remains unknown. The aim of this study was to evaluate the effects and underlying mechanisms of miR‑125a‑5p in MCF‑7 breast cancer cells. MCF‑7 cells were transfected with miR‑125a‑5p mimic or miR‑125a‑5p small interfering RNA to produce miR‑125a‑5p overexpressing/knockdown cells. Cell proliferation was assessed by an MTT assay, and cell migration ability was determined by an in vitro scratch assay. Hoechst 33258 staining and flow cytometry were performed to assess the effects of miR‑125a‑5p on MCF‑7 apoptosis. Western blotting and reverse transcription‑quantitative polymerase chain reaction were used for measuring phosphatase and tensin homolog (PTEN), phosphorylated (p)‑mitogen‑activated protein kinase kinase (MEK1/2)/MEK1/2, p‑ERK1/2/ERK1/2, B‑cell lymphoma‑2 (Bcl‑2), cleaved caspase‑3, and miR‑125a‑5p expression. miR‑125a‑5p overexpression inhibited the proliferation and migration, but promoted the apoptosis of MCF‑7 cells. These effects were associated with increases in PTEN and cleaved caspase‑3 expression, and decreases in p‑MEK1/2/MEK1/2, p‑ERK1/2/ERK1/2, and Bcl‑2. Silencing of miR‑125a‑5p exhibited opposing effects on MCF‑7 cells. These observations suggested that miR‑125a‑5p participates in the regulation of multiple functions of MCF‑7 cells by promoting the expression of PTEN tumor suppressor genes, activating MEK1/2/ERK1/2 signaling, and regulating caspase‑3/Bcl‑2 signaling. Thus, it may be a suitable target for breast cancer gene therapy.
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http://dx.doi.org/10.3892/mmr.2019.10704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797945PMC
November 2019

Characterization of the prognostic values of the family in gastric cancer.

Therap Adv Gastroenterol 2019 21;12:1756284819858507. Epub 2019 Jul 21.

Department of Gastrointestinal Surgery, Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China.

Background: The N-myc downstream-regulated gene () family, , has been involved in a wide spectrum of biological functions in multiple cancers. However, their prognostic values remain sparse in gastric cancer (GC). Therefore, it is crucial to systematically investigate the prognostic values of the family in GC.

Methods: The prognostic values of the family were evaluated by Kaplan-Meier Plotter and SurvExpress. The mRNA of the family was investigated in The Cancer Genome Atlas (TCGA). Transcription factors (TFs) and miRNAs associated with the family were predicted by NetworkAnalysis. The prognostic values of DNA methylation levels were analyzed by MethSurv. The correlation between immune cells and the family was evaluated by the Tumor Immune Estimation Resource (TIMER) database.

Results: High levels of mRNA expression of and were associated with a favorable prognosis in all GCs. In GC, was significantly associated with a poor prognosis of GC [hazard ratio (HR) = 1.65, 95% confidence interval (CI) = 1.16-2.33,  = 0.0046]. In GC, showed a poor prognosis (HR = 1.4, 95% CI: 1.06-1.85,  = 0.017). was an independent prognostic factor in recurrence-free survival by TCGA cohort. The low-risk -signature group displayed a significantly favorable survival outcome than the high-risk group (HR = 1.76, 95% CI: 1.2-2.59,  = 0.00385). The phosphorylated protein NDRG1 (NDRG1_pT346) displayed a favorable overall survival and was significantly associated with and phosphorylated . Epidermis development was the top biological process (BP) for coexpressed genes associated with and , while mitotic nuclear division and mitotic cell processes were the top BPs for and , respectively. Overall, 6 CpGs of , 4 CpGs of , 3 CpGs of and 24 CpGs of were associated with significant prognosis. CD4 T-cells showed the highest correlation with (correlation = 0.341,  = 2.14e). Furthermore, BCL6 in follicular helper T-cells (Tfh) cells showed the highest association with (correlation = 0.438,  = 00e00).

Conclusions: This study analyzed the multilevel prognostic values and biological roles of the family in GC.
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http://dx.doi.org/10.1177/1756284819858507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6647212PMC
July 2019

Safety and efficacy of leadless pacemaker retrieval.

J Cardiovasc Electrophysiol 2019 09 28;30(9):1671-1678. Epub 2019 Jul 28.

R&D Department, National United Engineering Laboratory for Biomedical Material Modification, Dezhou, China.

Background And Objectives: The success rate of leadless cardiac pacemaker (LP) retrieval remains a major concern for this disruptive technology. The present paper performed a systematic review of the safety and feasibility of the retrieval of LPs.

Methods: Primary publications that performed LP retrieval were collected and included five animal experiments and two worldwide retrieval experiences in human. The procedural details, such as indication, days post implantation, extraction success rate, and complications, were described. The present paper analyzed factors affecting the retrieval and management of the nonfunctional devices.

Results: Retrieval animal models was possible at least up to 2.5 years post implantation, and data from humans suggest that removal of a device that was implanted longer (eg, 4 years and 9 months for Nanostim; 4 years for Micra) could be performed within a reasonable safety profile. The fixed mechanism, implant site, and encapsulation of the LP systems may affect the retrieval process.

Conclusions: A high success rate in the relatively chronic retrieval of LPs was demonstrated, which promotes the extensive use of these devices in the treatment arrhythmia in the future.
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http://dx.doi.org/10.1111/jce.14076DOI Listing
September 2019

The Effect and Mechanism of Vascular Endothelial Growth Factor (VEGF) on Tumor Angiogenesis in Gallbladder Carcinoma.

Iran J Public Health 2019 Apr;48(4):713-721

Department of Surgery (I), Yiling Hospital of Yichang, Yichang 443100, P.R. China.

Background: To investigate the effect of vascular endothelial growth factor (VEGF) on tumor angiogenesis in gallbladder carcinoma.

Methods: Fifty one patients with gallbladder carcinoma were enrolled as observation group. Thirty healthy people were included as control group. Chemically synthesized siRNA sequences targeting VEGF was transfected with VEGF-siRNA. A blank group (group B), a negative control group (transfected with independent sequence, group C), and an inhibition group (transfected with VEGF siRNA, group D) were established. Physiological saline was set as group A. The expression of VEGF was detected by qRT-PCR. The expression of VEGF protein was detected by Western blot. MVD was used to measure microvessel density. CCK-8, Transwell and flow cytometry were used to detect cell proliferation, invasion and apoptosis.

Results: The tumor volume of nude mice and VEGF mRNA expression in group D was significantly smaller than that in group B and C (<0.05). The MVD density in group B and C was significantly higher than that in group D (<0.01). The proliferation of cells was detected from the 3rd day, and the proliferation of cells in the blank and negative control groups was faster than that of the inhibition group (<0.05). The apoptosis rate of the blank group and the negative control group was lower than that of the inhibition group (<0.001).

Conclusion: VEGF is highly expressed in serum of patients with cholangiocarcinoma, it promotes angiogenesis, proliferation and invasion of gallbladder cancer cells, and inhibits apoptosis of tumor cells.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6500531PMC
April 2019

Online Spatial and Temporal Calibration for Monocular Direct Visual-Inertial Odometry.

Sensors (Basel) 2019 May 16;19(10). Epub 2019 May 16.

Information Engineering University, Zhengzhou 450001, China.

Owing to the nonlinearity in visual-inertial state estimation, sufficiently accurate initial states, especially the spatial and temporal parameters between IMU (Inertial Measurement Unit) and camera, should be provided to avoid divergence. Moreover, these parameters are required to be calibrated online since they are likely to vary once the mechanical configuration slightly changes. Recently, direct approaches have gained popularity for their better performance than feature-based approaches in little-texture or low-illumination environments, taking advantage of tracking pixels directly. Based on these considerations, we perform a direct version of monocular VIO (Visual-inertial Odometry), and propose a novel approach to initialize the spatial-temporal parameters and estimate them with all other variables of interest (IMU pose, point inverse depth, etc.). We highlight that our approach is able to perform robust and accurate initialization and online calibration for the spatial and temporal parameters without utilizing any prior information, and also achieves high-precision estimates even when large temporal offset occurs. The performance of the proposed approach was verified through the public UAV (Unmanned Aerial Vehicle) dataset.
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http://dx.doi.org/10.3390/s19102273DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567321PMC
May 2019

lncINS-IGF2 Promotes Cell Proliferation and Migration by Promoting G1/S Transition in Lung Cancer.

Technol Cancer Res Treat 2019 01;18:1533033818823029

1 Clinical Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.

Long noncoding RNAs are capable of regulating gene expression at multiple levels. These RNA molecules are also involved in a variety of physiological and pathological processes. Emerging data demonstrate that a series of differentially expressed long noncoding RNAs are implicated in tumorigenesis. In the present study, we used microarray analysis to identify long noncoding RNAs that are dysregulated in non-small-cell lung cancer when compared to normal lung tissues. Accordingly, we performed quantitative real-time polymerase chain reaction to analyze the levels of long noncoding RNA and the cis target gene. We further found the oncogene property of long noncoding RNA that long noncoding RNA downexpression inhibits non-small-cell lung cancer cells proliferation and migration based on 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide and colony formation assays and wound healing as well as transwell assays. The influence of long noncoding RNA on cell cycle of non-small-cell lung cancer cells is also analyzed by flow cytometry. Among the dysregulated long noncoding RNAs, we identified INS-IGF2 readthrough, transcript variant 1, noncoding RNA (NR_003512.3) is upregulated in non-small-cell lung cancer tissues, the cis gene of which is insulin-like growth factor 2 gene hinted by bioinformatics analysis. We also observed that downregulation of INS-IGF2 readthrough, transcript variant 1, noncoding RNA reduces insulin-like growth factor 2 messenger RNA expression. Furthermore, INS-IGF2 readthrough, transcript variant 1, noncoding RNA downregulation suppresses non-small-cell lung cancer cell proliferation and migration. This downregulation results in a concomitant inhibition of the G1/S transition in non-small-cell lung cancer cells. Our findings suggest that INS-IGF2 readthrough, transcript variant 1, noncoding RNA may be an oncogene involved in the development of lung cancer. Therefore, we speculate that INS-IGF2 readthrough, transcript variant 1, noncoding RNA represents a potential therapeutic target for lung cancer.
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http://dx.doi.org/10.1177/1533033818823029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374000PMC
January 2019