Publications by authors named "Jianwei Lu"

117 Publications

Linc00941 regulates esophageal squamous cell carcinoma via functioning as a competing endogenous RNA for miR-877-3p to modulate PMEPA1 expression.

Aging (Albany NY) 2021 07 13;13(13):17830-17846. Epub 2021 Jul 13.

Department of Medical Oncology, Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and the Affiliated Cancer Hospital of Nanjing Medical University, Nanjing 210009, China.

Esophageal squamous cell carcinoma (ESCC) represents one of the most common malignancies and is the fifth leading cause of cancer-related deaths. Long intergenic non-coding RNAs (lincRNAs) have been suggested to be dysregulated in various types of cancers, and a growing number of lincRNAs have been implicated to be functional in the ESCC progression. In this study, we examined the role of linc00941 in the ESCC progression and explored the underlying molecular mechanisms. The bioinformatics analysis identified the up-regulation of linc00941 in the ESCC tissues. Further studies showed that linc00941 was up-regulated in ESCC cell lines. The loss-of-function studies demonstrated that linc00941 knockdown suppressed ESCC cell proliferation, invasion and migration, and also suppressed the tumor growth. Furthermore, bioinformatics prediction along with luciferase reporter assay and RNA immunoprecipitation assay implied that linc00941 acted as a competing endogenous RNA for miR-877-3p, and linc00941 regulated ESCC cell progression via at least targeting miR-877-3p. Subsequently, miR-877-3p targeted prostate transmembrane protein, androgen induced 1 (PMEPA1) 3' untranslated region and repressed PMEPA1 expression in ESCC cells; overexpression of PMEPA1 attenuated the inhibitory effects of linc00941 knockdown on the ESCC cell progression. Linc00941 knockdown suppressed epithelial-mesenchymal transition (EMT) via targeting miR-877-3p/PMEPA1 axis in ESCC cells. In conclusion, our results indicated the oncogenic role of linc00941 in ESCC, and knockdown of linc00941 suppressed ESCC cell proliferation, invasion, migration and EMT via interacting with miR-877-3p/PMEPA1 axis.
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http://dx.doi.org/10.18632/aging.203286DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312468PMC
July 2021

Knockdown of lncRNA TUC338 inhibits esophageal cancer cells migration and invasion.

J Thorac Dis 2021 May;13(5):3061-3069

Department of General Surgery, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China.

Background: Long non-coding RNAs (lncRNAs) are firmly identified with the event and improvement of tumors. Therefore, elucidating the functions and mechanisms of related lncRNAs is significant for understanding the occurrence and advancement of tumors. The recently discovered lncRNA TUC338 has been shown to play the role of an oncogene in an assortment of tumors. Be that as it may, the articulation and elements of lncRNA TUC338 in esophageal cancer are as yet hazy. This investigation plans to explain the capacities and related molecular mechanisms of lncRNA TUC338 in esophageal malignancy.

Methods: Firstly, the expression of TUC338 in 50 instances of esophageal disease tissues and nearby tissues was detected by fluorescence reckonable PCR, and correlations with the clinic pathological characteristics of patients was further analyzed. Then, a lentiviral interference vector was designed and transfected into an esophageal cancer cell line, and knockdown was verified by fluorescence quantitative PCR. The effects of TUC338 knockdown on the proliferation, clone formation, and migration and infringement of esophageal malignancy cells were tested utilizing the CCK-8 assay, clone formation experiments, and Transwell experiments. Western blot detected the expression of invasion-related proteins.

Results: Fluorescence reckonable PCR exhibit that TUC338 was exceptionally communicated in esophageal cancer tissues, and was significantly related with metastasis and TNM stage in tolerant. Functional experiments showed that in esophageal disease cell lines, knocking down the declaration of TUC338 significantly inhibited cell multiplication, clone development, and intrusion and movement. Further experiments on molecular mechanisms showed that knocking down TUC338 inhibited statement of N-cadherin and vimentin in cells.

Conclusions: TUC338 is exceptionally communicated in esophageal malignancy tissues and can regulate cell proliferation and invasion.
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http://dx.doi.org/10.21037/jtd-21-563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182530PMC
May 2021

Machine Learning Identifies Metabolic Signatures that Predict the Risk of Recurrent Angina in Remitted Patients after Percutaneous Coronary Intervention: A Multicenter Prospective Cohort Study.

Adv Sci (Weinh) 2021 05 8;8(10):2003893. Epub 2021 Mar 8.

School of Medicine University of California San Diego CA 92093 USA.

Recurrent angina (RA) after percutaneous coronary intervention (PCI) has few known risk factors, hampering the identification of high-risk populations. In this multicenter study, plasma samples are collected from patients with stable angina after PCI, and these patients are followed-up for 9 months for angina recurrence. Broad-spectrum metabolomic profiling with LC-MS/MS followed by multiple machine learning algorithms is conducted to identify the metabolic signatures associated with future risk of angina recurrence in two large cohorts ( = 750 for discovery set, and = 775 for additional independent discovery cohort). The metabolic predictors are further validated in a third cohort from another center ( = 130) using a clinically-sound quantitative approach. Compared to angina-free patients, the remitted patients with future RA demonstrates a unique chemical endophenotype dominated by abnormalities in chemical communication across lipid membranes and mitochondrial function. A novel multi-metabolite predictive model constructed from these latent signatures can stratify remitted patients at high-risk for angina recurrence with over 89% accuracy, sensitivity, and specificity across three independent cohorts. Our findings revealed reproducible plasma metabolic signatures to predict patients with a latent future risk of RA during post-PCI remission, allowing them to be treated in advance before an event.
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http://dx.doi.org/10.1002/advs.202003893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132066PMC
May 2021

Potassium modulates central carbon metabolism to participate in regulating CO transport and assimilation in Brassica napus leaves.

Plant Sci 2021 Jun 24;307:110891. Epub 2021 Mar 24.

College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River) Ministry of Agriculture and Rural Affairs, Wuhan 430070, China. Electronic address:

Potassium (K) regulates plant metabolism and enhances plant's ability to adapt to adversity. However, under different K deficiency stress, the net photosynthetic rate (A) was reduced, influenced by CO conductance or biochemical capacities. The interplay between metabolome and photosynthetic characteristics under K deficiency stress was analyzed to explore the mechanisms by which K regulates photosynthetic capacity. With increasing K deficiency stress, dominations limiting A varied from CO conductance to biochemical limitations. Multivariate analyses indicated that organic acids, amino acids and sedoheptulose-7-bisphosphate were significantly related to A, CO conductance and carboxylation rate. Under moderate K deficiency, organic acids were up-regulated. Acidification of subcellular compartments reduced sedoheptulose-1,7-bisphosphatase activity, inducing downregulation of sedoheptulose-7-bisphosphate and hindrance of ribulose bisphosphate regeneration. Moreover, increased CO shortage with increasing K deficiency induced a shift of increased citric acid to amino acid synthesis, causing excessive accumulation of amino acids. In addition, the reduced serine level indicated impaired photorespiration. These two changes triggered more serious reduction in photosynthetic capacity. The intimate, changes in photosynthetic capacities were tightly coupled with shifts in central C metabolism, which provides insights into the methods used to enhance A and plant's adaptability to abiotic stresses, through the regulation of C metabolites using molecular technology.
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http://dx.doi.org/10.1016/j.plantsci.2021.110891DOI Listing
June 2021

[Retracted] Regulation of the cell cycle and PI3K/Akt/mTOR signaling pathway by tanshinone I in human breast cancer cell lines.

Mol Med Rep 2021 06 13;23(6). Epub 2021 Apr 13.

First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210046, P.R. China.

Following the publication of the above paper, a concerned reader drew to the Editor's attention that Fig. 5 contained apparent anomalies, including unexpectedly similar-looking cells and repeated patternings of the cells in terms of their layout/arrangement within the data panels. After having conducted an independent investigation in the Editorial Office, the Editor of has determined that the above paper should be retracted from the Journal on account of a lack of confidence regarding the authenticity of the data. The authors were asked for an explanation to account for these concerns, but the Editorial Office never received any reply. The Editor regrets any inconvenience that has been caused to the readership of the Journal. [the original article was published in 11: 931‑939, 2015; DOI: 10.3892/mmr.2014.2819].
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http://dx.doi.org/10.3892/mmr.2021.12080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060802PMC
June 2021

Kinetics of plasma cfDNA predicts clinical response in non-small cell lung cancer patients.

Sci Rep 2021 Apr 7;11(1):7633. Epub 2021 Apr 7.

DiaCarta, Inc., 2600 Hilltop Drive, Richmond, CA, 94806, USA.

Tyrosine kinase inhibitors (TKIs), VEGF/VEGF receptor inhibitors (VEGFIs) and immune checkpoint inhibitors (ICIs) have revolutionized the treatment of advanced cancers including non-small-cell lung cancer (NSCLC). This study aims to evaluate the utility of plasma cell-free DNA (cfDNA) as a prognostic biomarker and efficacy predictor of chemotherapy (CT) with or without these precision therapies in NSCLC patients. Peripheral cfDNA levels in 154 NSCLC patients were quantified before and after the first target cycle of chemotherapy. The correlations of cfDNA with tumor burden, clinical characteristics, progression-free survival (PFS)/disease-free survival (DFS), objective response ratio (ORR), and therapy regimens were analyzed respectively. Baseline cfDNA, but not post-chemotherapeutic cfDNA, positively correlates with tumor burden. Notably, cfDNA kinetics (cfDNA Ratio, the ratio of post-chemotherapeutic cfDNA to baseline cfDNA) well distinguished responsive individuals (CR/PR) from the non-responsive (PD/SD). Additionally, cfDNA Ratio was found negatively correlated with PFS in lung adenocarcinoma (LUAD), but not lung squamous-cell carcinoma (LUSC) which may be due to a limited number of LUSC patients in this cohort. LUAD patients with low cfDNA Ratio have prolonged PFS and improved ORR, compared to those with high cfDNA Ratio. When stratified by therapy regimen, the predictive value of cfDNA Ratio is significant in patients with chemotherapy plus VEGFIs, while more patients need be included to validate the value of cfDNA Ratio in other regimens. Thus, the kinetics of plasma cfDNA during chemotherapy may function as a prognostic biomarker and efficacy predictor for NSCLC patients.
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http://dx.doi.org/10.1038/s41598-021-85797-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027214PMC
April 2021

IL-33 Promotes the Growth of Non-Small Cell Lung Cancer Cells Through Regulating miR-128-3p/CDIP1 Signalling Pathway.

Cancer Manag Res 2021 12;13:2379-2388. Epub 2021 Mar 12.

Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 210009 Jiangsu Province, People's Republic of China.

Background: Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer-related deaths, and it is also the most frequently diagnosed cancer. Previous studies indicate that IL-33 plays a crucial role in the development of NSCLC. In recent years, the role of miRNAs in cancer has become increasingly clear. However, reports focused on the relation between IL-33 and miRNAs in NSCLC have been limited.

Methods: The expression of IL-33 and miR-128-3p was detected by qPCR. MTT, EdU, and colony formation assays were used to detect the proliferation ability of NSCLC cells. Transwell assay was used to investigate the migration and invasion of NSCLC cells. The expression of bax, cyt-c, and caspase 3 was detected by Western blot. Finally, in vivo tumor xenograft was used to detect the effects of IL-33 and miR-128-3p on tumor growth capacity.

Results: IL-33 was notably increased in the serum and tumor tissue of NSCLC patients. The in vitro function study revealed that IL-33 significantly promotes the proliferation, migration, and invasion of the NSCLC cells. In vivo experiments further confirmed the pro-tumor effect of IL-33 on NSCLC. The study on the underlying mechanism elucidated that IL-33 regulates the expression of miR-128-3p, which can directly target and inhibit the expression of CDIP1. Furthermore, IL-33 regulates the expression of downstream apoptotic proteins such as bax, cyt-c, and caspase3. Rescue experiments demonstrated that miR-28-3p can reverse the effect of IL-33.

Conclusion: These findings indicated that IL-33 and miR-128-3p may play a potential role in the diagnosis and treatment of NSCLC.
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http://dx.doi.org/10.2147/CMAR.S276297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7965692PMC
March 2021

Down-regulation of estrogen-related receptor alpha (ERRα) inhibits gastric cancer cell migration and invasion and .

Aging (Albany NY) 2021 02 11;13(4):5845-5857. Epub 2021 Feb 11.

Department of Medical Oncology, Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing 210009, Jiangsu Province, China.

Objective: To investigate the correlation between estrogen-related receptor a (ERRα) expression level and gastric cancer (GC).

Methods: We collected GC and adjacent normal tissues from 50 patients. The parameters of the patients were summarized, and correlation with the expression level of ERRα was calculated. Downregulated ERRα using lentivirus was designed and transfected to SGC-7901 and MGC-803 cells. Cell migration, invasion and wound assays were conducted to determine the correlation between ERRα and capacity for cell migration and invasion. The expression level of the genes involved in epithelial-mesenchymal transition, including E-cadherin, γ-catenin, N-cadherin and vimentin, was determined via real-time or quantitative polymerase chain reaction(qPCR) and Western blot analysis.

Results: The expression of ERRα tends to be higher in GC tissues than in adjacent normal tissues. Analyses ofthe expression level of ERRα and patient parameters show that the ERRα level is significantly correlated with TNM staging and patient survival (<0.05). The downregulation of ERRα can inhibit cell invasion and migration, which was proven by Transwell and cell wound assays. The levels of E-cadherin and γ-catenin increased by conducting qPCR and Western blot analysis. Meanwhile, the levels of N-cadherin and vimentin decreased when ERRα expression was reduced.

Conclusion: ERRα is highly expressed in GC tissues and can promote the migration and invasion of cancer cells. It can be a potential marker for GC diagnosis.
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http://dx.doi.org/10.18632/aging.202508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950300PMC
February 2021

Retraction Note to: Inhibition of ZEB1-AS1 confers cisplatin sensitivity in breast cancer by promoting microRNA-129-5p-dependent ZEB1 downregulation.

Cancer Cell Int 2021 Jan 11;21(1):40. Epub 2021 Jan 11.

Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing, Medical University, No. 42 Baiziting, Nanjing, 210009, Jiangsu, People's Republic of China.

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http://dx.doi.org/10.1186/s12935-021-01759-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798271PMC
January 2021

KRAS Codon 12 Mutation is Associated with More Aggressive Invasiveness in Synchronous Metastatic Colorectal Cancer (mCRC): Retrospective Research.

Onco Targets Ther 2020 8;13:12601-12613. Epub 2020 Dec 8.

The Department of Oncology, The Affiliated Cancer Hospital of Nanjing Medical University, and Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research, Nanjing, People's Republic of China.

Objective: To investigate the connection between mutant and clinicopathological characteristics in therapy-naïve synchronous metastatic colorectal cancer (mCRC) in Chinese populations when compared with all wild type ( wild type).

Patients And Methods: A total of 200 patients with therapy-naïve synchronous mCRC (TNM stage: TanyNanyM1) were retrospectively collected as study objects. Primary tumor tissues from 200 mCRC patients were analyzed through next-generation sequencing panel to assess the mutated regions of .

Results: The distribution frequency of gene mutation in our study was 41% , 4% , 11.5% , 0.5% both and . Tumors with any gene mutations (any gene mutations in ), and mutation were more likely to be located in right-sided colon (P=0.007, P=0.008, P=0.026, respectively). For metastasis, tumors with any gene mutations, and mutation were significantly correlated with peritoneal metastasis (P=0.019, P=0.017, P=0.014, respectively), liver-peritoneum metastases (P=0.004, P=0.003, P=0.002, respectively) and multi-organ metastases (P=0.002, P=0.008, P=0.001, respectively). Tumors with all wild type were significantly correlated with distant lymph node-only metastasis. No statistically significant differences were found between clinicopathological characteristics and and mutations.

Conclusion: Our study suggests that clinicopathological characteristics (specifically for metastasis) are related to mutations in therapy-naïve synchronous mCRC population in China. We demonstrated that distant lymph node-only metastasis is visibly linked to all wild-type tumors. We found that patients with any gene mutations, mutation are more likely to carry peritoneal metastasis, liver-peritoneum metastases and multi-organ metastases than those with all wild type. After stratification, mutation, but not mutation, was remarkably associated with peritoneal metastasis, liver-peritoneum metastases, and multi-organ metastases compared to all wild type. These results may be useful for aiding in the prediction of prognosis and choosing the appropriate regimens for therapy.
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http://dx.doi.org/10.2147/OTT.S279312DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7737549PMC
December 2020

Optimal potassium management strategy to enhance crop yield and soil potassium fertility under paddy-upland rotation.

J Sci Food Agric 2021 Jun 7;101(8):3404-3412. Epub 2021 Jan 7.

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, Wuhan, China.

Background: An unbalanced application of potassium (K) fertilizer usually destabilizes crop yield and affects soil K fertility. Developing a sustainable K management strategy requires improvements in crop yield without reducing soil K supply capacity over the long term. A combination of field experiments of K fertilization and straw return using rice (Oryza sativa L.)-oilseed rape (Brassica napus L.) rotation was designed to develop an optimal K management strategy.

Results: The results showed the best strategy to maintain yield was K +S (input equivalent K removed by seed treatment and straw return), K +K (input equivalent K removed by straw and seed) and K +S (conventional K fertilization and straw return) treatments, and the yield gap among different treatments expanded with the extension of planting years. There were significant differences present in rice and rape K uptake, although no differences in seed K uptake were observed under different K management strategies. The K balance was approximately maintained under K +S and K +K treatments, and negative K balances were present for K (no K application), K (conventional fertilization), +S (straw return) and K treatments (input equivalent K that removed by straw treatment). A positive balance was observed under K +S treatment. Slight changes in soil exchangeable and nonexchangeable K were observed under K +S and +S treatments. However, high inputs of K fertilizer prevented the improvement of agronomic efficiency and recovery efficiency of K.

Conclusions: In summary, the optimal K management strategy was K +S, which stabilizes the crop yield, maintains soil K fertility and maximizes K use efficiency. © 2020 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.10970DOI Listing
June 2021

Ontogeny of hepatic methionine catabolic enzyme activities (Transmethylation and Transsulphuration) and associated physiological amino acids in E10-21 chick embryos and D1-49 broilers.

J Anim Physiol Anim Nutr (Berl) 2021 May 7;105(3):507-519. Epub 2020 Nov 7.

Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR, USA.

Developmental changes in hepatic methionine adenosyltransferase, cystathionine β-synthase, cystathionase, and glycine N-methyltransferase were determined in broiler chick embryos and hatched chicks by using radiometric and spectrometric methods. Hepatic free methionine, S-adenosylmethionine, S-adenosylhomocysteine, homocysteine, cystathionine, and cysteine levels were also investigated. Results showed an increase in hepatic MAT activity from E10 to E21 during embryogenesis, suggesting greater transmethylation rates throughout the rapid embryonic growth and development period. A strong positive correlation between embryo BW and MAT activity also supports this idea. The MAT specific activity continued to increase after hatching, but there was a negative correlation between chick BW and MAT activities from D1 to D49. This may indicate different MAT isozymes exist for chick embryo hepatic tissue compared to hepatic tissue of hatched chick and growing broilers. The developmental pattern of MAT isozymes could be critical for methionine metabolism to cope with the demand imposed on the embryo, chicks, and growing broilers. Additionally, the specific activity of hepatic CBS in chick embryos was determined to be lower compared to that observed in older broilers (35 and 49 days). Since liver CBS specific activity is at the lowest point from D1-7 in young chicks, the ability to convert adequate homocysteine to cysteine through transsulphuration may be limiting for cysteine synthesis at this time. Steady-state hepatic homocysteine levels in chick embryos and chicks may be a function of the rates of homocysteine formation, remethylation, and catabolism via the transsulphuration pathway. The present study indicates young chicks from D1 to D7 may have a limited ability for adequate transsulphuration; therefore, dietary cystine may be needed for optimum performance.
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http://dx.doi.org/10.1111/jpn.13463DOI Listing
May 2021

Author Correction: Plasma activity of Thioredoxin Reductase as a Novel Biomarker in Gastric Cancer.

Sci Rep 2020 Oct 14;10(1):17254. Epub 2020 Oct 14.

Keaise Center for Clinical Laboratory, Wuhan, China.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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http://dx.doi.org/10.1038/s41598-020-70071-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7560690PMC
October 2020

The hypoglycemic and renal protective effects of Grifola frondosa polysaccharides in early diabetic nephropathy.

J Food Biochem 2020 12 11;44(12):e13515. Epub 2020 Oct 11.

Faculty of Medicine, Macau University of Science and Technology, Taipa, Macau, China.

Grifola frondosa is a basidiomycete polypore fungus. Polysaccharides from G. frondosa (PGF) has recently attracted attention for its various physiological activities including antioxidant, antitumor, and anti-fatigue effects. In this study, hypoglycemic activity of PGF and its preventive effect against the progression of kidney fibrosis in Diabetic nephropathy (DN) rats were investigated. The results showed that PGF led to a significant decrease in fasting blood glucose levels and an increase in body weight in the treatment group compared with those of model group. Serum biochemical indexes including N-acetyl-β-D-glucosaminidase (NAG), blood urea nitrogen (BUN), serum creatinine (SCr), and urine microalbumin (u-mAlb) levels of PGF-treated group were significantly lower than those of model group. Inflammatory cytokines and renal fibrosis indexes of PGF group were also decreased compared to the model group. The whole results demonstrated the renal-protective effects of PGF via reducing the inflammatory factor content and preventing renal fibrosis. PRACTICAL APPLICATIONS: G. frondosa constitutes a rich source of polysaccharides, steroid, and phenolic compounds. The results obtained revealed that the purified PGF have the effect of reducing inflammation cytokines and renal fibrosis indexes. These two factors are associated with the development and progression of diabetic nephropathy. Therefore, PGF may produce both hypoglycemic and renal-protective effects, and potentially be of use as a functional food for the treatment or prevention of diabetic nephropathy.
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http://dx.doi.org/10.1111/jfbc.13515DOI Listing
December 2020

A Retrospective Study of Neoadjuvant Chemotherapy for Locally Advanced Gastric Cancer.

Cancer Manag Res 2020 15;12:8491-8496. Epub 2020 Sep 15.

The Affiliated Cancer Hospital of Nanjing Medical University and Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research, Nanjing, People's Republic of China.

Objective: To explore the efficacy and safety of neoadjuvant chemotherapy in the doublet and triplet regimens of locally advanced gastric cancer.

Patients And Methods: A retrospective analysis was conducted on 162 patients with gastric cancer who received neoadjuvant chemotherapy, including 74 patients receiving doublet regimen (fluorouracil/platinum) and 88 patients receiving triplet regimen (fluorouracil/platinum/Taxol). Patients in both groups received neoadjuvant chemotherapy for two cycles, and underwent surgical resection 4 weeks after the end of chemotherapy.

Results: The total clinical remission rate was 68.6% (105/153), the phase-down rate was 46.4% (71/153), and the pathological response rate was 59.9% (97/162). In the doublet and triplet regimen, the clinical remission rate was 65.7% (44/67) and 70.9% (61/86) (P = 0.708), the descending period rate was 41.8% (28/67) and 50.0% (43/86) (P = 0.485), and the pathological response rate was 51.4% (38/74) and 67.0% (59/88) (P = 0.190). The median disease-free survival (DFS) and overall survival (OS) of 162 patients were 36.0 and 58.5 months. In the doublet and triplet regimen, the median DFS was 38.0 and 34.0 months (P = 0.377), and the median OS was 59.0 and 56.5 months (P = 0.256). The side effects of the doublet group were significantly lower than those of the triplet group, with leucopenia rate of 45.9% (34/74) and 62.5% (55/88) (P = 0.035); thrombocytopenia rate of 18.9% (14/74) and 35.2% (31/88) (P = 0.021); nausea rate of 45.9% (34/74) and 64.8% (57/88) (P = 0.016), and diarrhea rate of 1.4% (1/74) and 9.1% (8/88) (P = 0.032).

Conclusion: Neoadjuvant chemotherapy is safe and effective for locally advanced gastric cancer. The clinical efficacy of neoadjuvant chemotherapy in the doublet group and the triplet group is equivalent, and the doublet group has better safety and tolerance.
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http://dx.doi.org/10.2147/CMAR.S267330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501965PMC
September 2020

mA RNA modification modulates PI3K/Akt/mTOR signal pathway in Gastrointestinal Cancer.

Theranostics 2020 25;10(21):9528-9543. Epub 2020 Jul 25.

Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, 646000, Sichuan, PR China.

Methylation at the N6 position of adenosine (mA) is the most prevalent RNA modification within protein-coding mRNAs in mammals, and it is a reversible modification with various important biological functions. The formation and function of mA are regulated by methyltransferases (writers), demethylases (erasers), and special binding proteins (readers) as key factors. However, the underlying modification mechanisms of mA in gastrointestinal (GI) cancer remain unclear. Here, we performed comprehensive molecular profiling of the nine known mA writer, eraser, and reader proteins in GI cancer. Data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) were used. Gene alteration and pathway analysis were done in cBioportal. The protein network of mA regulators and its related pathway members was analyzed in STRING online platform. Phylogenetic tree was constructed in MEGA7. mA modification sites were predicted by SRAMP. mA related SNPs were analyzed by mASNP. The modulation of mA on its related pathway members was validated by mA-seq, real-time PCR and phosphor-MAPK array. We found that mA regulators were mostly upregulated in GI cancer and their differential expression significantly influenced the overall survival of patients with GI cancer. The phosphatidylinositol-3-kinase (PI3K)/Akt and mammalian target of rapamycin (mTOR) signaling pathways were found to be potentially affected by mA modification in most human cancers, including GI cancer, which was further verified by mA-Seq and phospho-MAPK array. Our findings suggest that mA RNA modification has a fundamental role in the regulation of PI3K/Akt and mTOR signaling pathway function in cancer.
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http://dx.doi.org/10.7150/thno.42971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7449908PMC
June 2021

LncRNA KTN1-AS1 promotes the progression of non-small cell lung cancer via sponging of miR-130a-5p and activation of PDPK1.

Oncogene 2020 09 20;39(39):6157-6171. Epub 2020 Aug 20.

Department of Medical Oncology, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital& Jiangsu Institute of Cancer Research, Nanjing, People's Republic of China.

Non-small cell lung cancer (NSCLC) is the major cause of cancer-associated death worldwide, but its underlying mechanisms remain to be fully elucidated. Long noncoding RNAs (lncRNAs) are known to play an important role in the aberrant regulation of gene expression in many cancers, including NSCLC. Here, we investigated the involvement of the lncRNA KTN1-AS1 in NSCLC. We found that KTN1-AS1 expression was upregulated in NSCLC tissue and was positively associated with poor prognosis. KTN1-AS1 knockdown inhibited cell growth and proliferation, increased apoptosis, and modulated the expression of cell cycle- and apoptosis-related proteins (cyclin A1, cyclin-dependent kinase 2, Bcl2, and Bax) in NSCLC cell lines and tumour xenografts in nude mice. KTN1-AS1 bound to and directly regulated the expression of miR-130a-5p. Notably, miR-130a-5p overexpression suppressed NSCLC cell proliferation and increased apoptosis in vitro and in vivo, and this effect was reversed by KTN1-AS1 overexpression. Finally, we showed that KTN1-AS1 modulated the expression of 3-phosphoinositide-dependent protein kinase 1 (PDPK1), a miR-130a-5p target and key regulator of autophagy in NSCLC cells. Taken together, our results suggest that the KTN1-AS1/miR-130a-5p/PDPK1 pathway may be a potential therapeutic target for NSCLC.
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http://dx.doi.org/10.1038/s41388-020-01427-4DOI Listing
September 2020

Nutrition-mediated cell and tissue-level anatomy triggers the covariation of leaf photosynthesis and leaf mass per area.

J Exp Bot 2020 10;71(20):6524-6537

Microelement Research Center, Huazhong Agricultural University, Wuhan, China.

Plants in nutrient-poor habitats converge towards lower rates of leaf net CO2 assimilation (Aarea); however, they display variability in leaf mass investment per area (LMA). How a plant optimizes its leaf internal carbon investment may have knock-on effects on structural traits and, in turn, affect leaf carbon fixation. Quantitative models were applied to evaluate the structural causes of variations in LMA and their relevance to Aarea in rapeseed (Brassica napus) based on their responses to nitrogen (N), phosphorus (P), potassium (K), and boron (B) deficiencies. Leaf carbon fixation decreased in response to nutrient deficiency, but the photosynthetic limitations varied greatly depending on the deficient nutrient. In comparison with Aarea, the LMA exhibited diverse responses, being increased under P or B deficiency, decreased under K deficiency, and unaffected under N deficiency. These variations were due to changes in cell- and tissue-level carbon investments between cell dry mass density (N or K deficiency) and cellular anatomy, including cell dimension and number (P deficiency), or both (B deficiency). However, there was a conserved pattern independent of nutrient-specific limitations-low nutrient availability reduced leaf carbon fixation but increased carbon investment in non-photosynthetic structures, resulting in larger but fewer mesophyll cells with a thicker cell wall but a lower chloroplast surface area appressed to the intercellular airspace, which reduced the mesophyll conductance and feedback-limited Aarea. Our results provide insight into the importance of mineral nutrients in balancing the leaf carbon economy by coordinating leaf carbon assimilation and internal distribution.
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http://dx.doi.org/10.1093/jxb/eraa356DOI Listing
October 2020

A Novel Nonsense Mutation in the Gene Identified in a Family with Hereditary Multiple Osteochondromas.

Genet Test Mol Biomarkers 2020 Aug 15;24(8):478-483. Epub 2020 Jul 15.

Department of Orthopedics and Joint Surgery, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, P.R. China.

Identification of genetic mutations linked to hereditary multiple osteochondromas (HMO) is crucial for understanding the molecular mechanisms leading to disease pathogenesis. In this study, we investigated four patients and eight healthy individuals from a family with HMO. Clinical HMO data and Sanger sequences of the coding regions of the exostosin glycosyltransferase 1 () gene (18q24.11) and the gene (11p12) of all 12 members of the family were analyzed. A novel nonsense mutation in the gene (c.526C>T; p.Gln176*) was detected, which was present in all four patients but absent in their healthy relatives. This mutation encodes a stop codon that results in a truncated EXT2 protein that consists of only 176 amino acids and lacks the remaining 522 amino acids at its C-terminus, missing the entire glycosyltransferase domain. Association of a truncated EXT2 protein with HMO provides new insights into exostosis pathogenesis, highlighting potential roles of the gene and its glycosyltransferase domain. Further research is required to understand the mechanisms underlying the development of exostosis.
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http://dx.doi.org/10.1089/gtmb.2020.0017DOI Listing
August 2020

Atomic Layer Deposition onto Thermoplastic Polymeric Nanofibrous Aerogel Templates for Tailored Surface Properties.

ACS Nano 2020 Jul 14;14(7):7999-8011. Epub 2020 Jul 14.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China.

Poly(vinyl alcohol--ethylene) (EVOH) nanofibrous aerogel (NFA) templates were fabricated through vacuum freeze-drying from EVOH nanofibrous suspensions. Aluminum oxide (AlO) layers were deposited onto highly porous templates to form organic-inorganic hybrid aerogels by the atomic layer deposition (ALD) technique. Chemical and physical measurements showed that mechanical properties were improved through ALD. In addition, the surface chemistry of ALD modified aerogels showed a fascinating cyclic change based on the number of ALD deposition cycles. A transition from hydrophilicity to hydrophobicity was observed after a few cycles of ALD coating; however, additional deposition cycles changed the wettability characteristics back to hydrophilicity. This hydrophilic-hydrophobic-hydrophilic variation is shown to be governed by a combination of geometrical and chemical surface properties. Furthermore, the deposited AlO could substantially improve aerogels strength and reduce permanent deformation after cyclic compression. The Young's modulus of aerogels increased from 5.54 to 33.27 kPa, and the maximum stress at 80% strain went up from 31.13 to 176.11 kPa, after 100 cycles of trimethyl-aluminum (TMA)/water ALD. Thermogravimetric analysis (TGA) results confirm that ALD can effectively improve the heat resistance characteristics of polymeric aerogel. The onset temperature and the residual mass increased with increasing numbers of ALD cycles. During pyrolysis, the nanofiber cores were decomposed, and the brittle pure AlO self-supporting nanotube aerogels with the continuous hollow nanotubular network were formed. A coating of continuous thickness AlO layer on individual nanofiber was achieved after 100 ALD cycles. In additional to mechanical strength and physical property changes, the ALD modified aerogel also shows a superhydrophobic and oleophilic surface chemistry, which could potentially be used to remove oils/organic solvents from water. The resultant aerogels exhibit excellent absorption capacity (31-73 g/g) for various liquids, and the material could be reused after distillation or squeezing. A successful scale-up of such materials could provide some insights into the design and development of thermoplastic polymeric NFAs with substantial industrial applications.
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http://dx.doi.org/10.1021/acsnano.9b09497DOI Listing
July 2020

Developmental changes in physiological amino acids and hepatic methionine remethylation enzyme activities in E10-21 chick embryos and D1-49 broilers.

J Anim Physiol Anim Nutr (Berl) 2020 Nov 27;104(6):1727-1737. Epub 2020 Jun 27.

Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, Arkansas.

The remethylation of homocysteine to methionine is important for chick embryos to sustain the S-adenosylmethionine transmethylation reactions, which are essential for the rapid proliferation of cells. Developmental changes in hepatic 5-methyltetrahydrofolate-homocysteine methyltransferase (MFMT), betaine-homocysteine methyltransferase (BHMT) and hepatic serine hydroxymethyltransferase (SHMT) were determined in E10-21 Cobb 500 broiler chick embryos and hatched chicks from D1-49. Hepatic levels of free serine, glycine, putrescine, spermidine and spermine levels were also determined. Analyses showed hepatic MFMT-specific activity doubled from E10 to E12, with remaining embryo development experiencing small fluctuations in activity through E21. Hepatic MFMT doubled immediately after hatch, with peak activity occurring at D3. Afterwards, hepatic MFMT-specific activity steadily declined from D7-49. Hepatic BHMT activity was higher from E10 to E16 of embryogenesis, decreased rapidly at E17 and remained lower through E21 (p < .05). Hepatic BHMT-specific activity was also lower in chicks, with the exception of a peak in specific activity on D7. BHMT activity returned to lower levels by D21. Throughout embryogenesis, hepatic SHMT activity in chick embryos remained relatively constant except for a decrease at 13E, followed by an increase at 14E. Maximal activity of SHMT was found the first day post-hatch. Additionally, SHMT activity was significantly lower in growing chicks than that in embryos. Hepatic-free serine and glycine levels were negatively correlated with SHMT in hatched chicks. Hepatic polyamine, putrescine and spermidine shared a similar development pattern: peak level in the middle of incubation, low at late embryogenesis and lowest during the post-hatch period except an increase within one week after hatch. The sharp increase in hepatic concentrations of glycine, serine and putrescine, along with increased specific activities of MHMT, BHMT and SHMT from D1-7, suggests that methionine conservation (remethylation from homocysteine) and glycine/serine is critical for young chicks for organ growth, maturation, and development.
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http://dx.doi.org/10.1111/jpn.13390DOI Listing
November 2020

Clinical application of the AUC-guided dosage adjustment of docetaxel-based chemotherapy for patients with solid tumours: a single centre, prospective and randomised control study.

J Transl Med 2020 06 8;18(1):226. Epub 2020 Jun 8.

Department of Medical Oncology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, No. 42, Baiziting, Nanjing, 210009, China.

Background: Docetaxel (DTX) is a widely used anti-tumour drug, and its dosage is solely determined by body surface area (BSA). Adverse events, such as neutropenia or unsatisfied efficacy, likely occur because of differences in the pharmacokinetics (PK) and pharmacodynamics of patients. Thus, a feasible dosage adjustment method is needed.

Methods: A total of 209 eligible patients who provided consent were enrolled and randomised into two groups to receive the BSA- and PK-guided dosage adjustments of DTX-based chemotherapy (3 weeks per cycle). The AUC of DTX was detected, and the therapeutic window for Chinese patients was determined. The proportion of patients within the therapeutic window was evaluated. Neutropenia was examined in accordance with the toxicity grading standard suggested by the World Health Organisation. Tumour response was assessed in accordance with Response Evaluation Criteria in Solid Tumors version 1.1. The primary endpoint was the incidence of neutropenia, and the secondary endpoints were disease control rate (DCR) and 3-year survival rate.

Results: The therapeutic window for Chinese patients was 1.7-2.5 mg·h/L. The proportion of patients within the therapeutic window was 63.89% versus 28.33% (P < 0.0001), and the incidence of neutropenia was 68.33% versus 38.89% (P = 0.001) in the experimental group versus the control group in the sixth cycle, respectively. DCR was 72% versus 85% (P = 0.018) in the control group versus the experimental group. The 3-year survival rate of the PK group was significantly higher than that of the BSA group (P = 0.034).

Conclusions: The PK-guided dosage adjustment of DTX could significantly increase the proportion of patients within the therapeutic window, decrease the incidence of neutropenia and increase the DCR and the 3-year survival rate. The PK-guided dosage adjustment based on the dynamic monitoring of AUC could be a useful method for oncologists to improve individualised treatment options, optimise drug efficacy and reduce drug toxicity.
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http://dx.doi.org/10.1186/s12967-020-02394-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7282138PMC
June 2020

Plasma cfDNA as a Potential Biomarker to Evaluate the Efficacy of Chemotherapy in Gastric Cancer.

Cancer Manag Res 2020 5;12:3099-3106. Epub 2020 May 5.

Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and the Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, People's Republic of China.

Objective: To investigate the clinical value of plasma cell-free DNA (cfDNA) as a potential biomarker for advanced gastric cancer (GC).

Patients And Methods: One hundred and six cases of advanced gastric cancer patients receiving chemotherapy were selected as study objects. Another 40 healthy volunteers were included as control groups. Plasma cfDNA concentration was detected by (SuperbDNA) hybridization. Changes in cfDNA concentration during chemotherapy in patients with gastric cancer whose efficacy was assessed as partial response (PR), stable disease (SD) and disease progression (PD) were analyzed respectively. The relationship between the level of cfDNA and the efficacy of chemotherapy and clinical characteristics was also explored. In addition, cfDNA and other tumor markers were subjected to specificity and sensitivity analyses using ROC.

Results: cfDNA concentration in advanced GC patients was significantly higher than that in healthy controls (P<0.05). The concentration of plasma cfDNA in patients with PD showed an increasing trend over time. The concentration of plasma cfDNA in patients with therapeutic effect of PR decreased over time. In patients with therapeutic effect of SD, the plasma DNA concentration showed a stable trend over time. There was no significant correlation between cfDNA concentration and factors including gender, age, pathological type, CA724, CA125,CA199, AFP and CEA. ROC results showed that the area under the curve of cfDNA was larger than other tumor markers.

Conclusion: Plasma cfDNA concentration was significantly increased in patients with gastric cancer, and its diagnostic efficacy was superior to that of traditional tumor markers. It can be used as a tumor biomarker to monitor the efficacy of chemotherapy for gastric cancer.
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http://dx.doi.org/10.2147/CMAR.S243320DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211302PMC
May 2020

The reduction in leaf area precedes that in photosynthesis under potassium deficiency: the importance of leaf anatomy.

New Phytol 2020 09 8;227(6):1749-1763. Epub 2020 Jun 8.

College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, China.

Synergistic improvement in leaf photosynthetic area and rate is essential for enhancing crop yield. However, reduction in leaf area occurs earlier than that in the photosynthetic rate under potassium (K) deficiency stress. The photosynthetic capacity and anatomical characteristics of oilseed rape (Brassica napus) leaves in different growth stages under different K levels were observed to clarify the mechanism regulating this process. Increased mesophyll cell size and palisade tissue thickness, in K-deficient leaves triggered significant enlargement of mesophyll cell area per transverse section width (S/W), in turn inhibiting leaf expansion. However, there was only a minor difference in chloroplast morphology, likely because of K redistribution from vacuole to chloroplast. As K stress increased, decreased mesophyll surface exposed to intercellular space and chloroplast density induced longer distances between neighbouring chloroplasts (D ) and decreased the chloroplast surface area exposed to intercellular space (S /S); conversely this induced a greater limitation imposed by the cytosol on CO transport, further reducing the photosynthetic rate. Changes in S/W associated with mesophyll cell morphology occurred earlier than changes in S /S and D , inducing a decrease in leaf area before photosynthetic rate reduction. Adequate K nutrition simultaneously increases photosynthetic area and rate, thus enhancing crop yield.
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http://dx.doi.org/10.1111/nph.16644DOI Listing
September 2020

A clinical study of traditional Chinese medicine prolonging the survival of advanced gastric cancer patients by regulating the immunosuppressive cell population: A study protocol for a multicenter, randomized controlled trail.

Medicine (Baltimore) 2020 Apr;99(16):e19757

The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine.

Background: Gastric cancer (GC) is a common high-mortality disease, causing a serious social burden. Traditional Chinese medicine has been utilized to prevent and treat GC for many years but its effects remain unclear. The aim of our study is to elucidate the anti-tumor effects and the possible mechanism of Jianpi Yangzheng Xiaozheng decoction.

Methods/design: This is a prospective, multicenter, randomized controlled trial continuing 1.5 years. Two hundred ten eligible patients will be randomly divided into 2 groups, the chemotherapy alone and the chemotherapy combined with JPYZXZ group at a ratio of 1:2. All patients will receive the treatment for 24 weeks and follow up for 1.5 years. The primary outcomes are one-year survival rate, progression-free survival, and overall survival (OS), while the secondary outcomes are immune related hematology test, objective response rate, tumor makers, traditional Chinese medicine syndrome points, fatigue scale, and quality of life scale. All of these outcomes will be analyzed at the end of the trail.

Discussion: This study will provide the objective evidence for the efficacy and safety of Jianpi Yangzheng Xiaozheng decoction in advanced GC. Furthermore, it will be helpful to form a therapeutic regimen in advanced GC by the combination of traditional medicine and western medicine.Trail registration: ChiCTR1900028147.
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http://dx.doi.org/10.1097/MD.0000000000019757DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220101PMC
April 2020

Simultaneous bilateral femoral neck fractures in a dialysis-dependent patient: case report and literature review.

BMC Musculoskelet Disord 2020 Apr 15;21(1):242. Epub 2020 Apr 15.

Department of Orthopaedics, Tongde Hospital of Zhejiang Province, 234 GuCui Road, HangZhou, 310012, Zhejiang Province, China.

Background: Simultaneous bilateral femoral neck fractures are extremely rare without obvious injury. Herein, we report the case of a patient on dialysis presenting with bilateral femoral neck fractures, which is a condition with high complication and mortality rates according to a review of the pertinent literature.

Case Presentation: We report the case a 47-year-old female with a history of 8 years of haemodialysis due to polycystic kidney disease who presented with bilateral hip pain during walking. The clinical history and results of physical and radiographic examinations of this patient are shown. Single-stage bilateral hemiarthroplasty was performed after a multidisciplinary team consultation. Three days after the operation, she could ambulate with a walker. The woman gradually regained her previous ability to walk over 6 months after surgery.

Conclusions: A multidisciplinary team consultation for perioperative management is necessary and effective in patients on dialysis. Early diagnosis with prompt surgical treatment could lead to favourable recovery.
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http://dx.doi.org/10.1186/s12891-020-03281-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158116PMC
April 2020

Inhibition of ZEB1-AS1 confers cisplatin sensitivity in breast cancer by promoting microRNA-129-5p-dependent ZEB1 downregulation.

Cancer Cell Int 2020 23;20:90. Epub 2020 Mar 23.

Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, No. 42, Baiziting, Nanjing, 210009, Jiangsu, People's Republic of China.

Background: Breast cancer is the leading cause of cancer-related mortality in women worldwide. Long non-coding RNAs (lncRNAs) are of critical importance in tumor drug resistance. Herein, this study aims to determine the roles of lncRNA ZEB1-AS1 in drug resistance of breast cancer involving microRNA-129-5p (miR-129-5p) and ZEB1.

Methods: Microarray-based gene expression profiling of breast cancer was conducted to identify the differentially expressed lncRNAs. ZEB1 expression was measured in adjacent and cancerous tissues. Next, MCF-7 and MDA-MB-231 cells were treated with a series of inhibitor, mimic or siRNA to clarify the roles of lncRNA ZEB1-AS1 and miR-129-5p in drug resistance of breast cancer. Then the target relationship of miR-129-5p with lncRNA ZEB1-AS1 and ZEB1 was verified. The expression patterns of miR-129-5p, lncRNA ZEB1-AS1, -, -, ZEB1 and corresponding proteins were evaluated. Moreover, the apoptosis and drug resistance of MCF-7 cell were detected by CCK-8 and flow cytometry respectively.

Results: LncRNA ZEB1-AS1 was observed to be an upregulated lncRNA in breast cancer, and ZEB1 overexpression was noted in breast cancerous tissues. MiR-129-5p was revealed to specifically bind to both ZEB1 and lncRNA ZEB1-AS1. Moreover, the expression levels of ZEB1-AS1, ZEB1, -, -, and corresponding proteins were decreased, but the expression of miR-129-5p was increased with transfection of miR-129-5p mimic and lncRNA ZEB1-AS1 siRNA. Besides, drug resistance to cisplatin was inhibited, and cell apoptosis was promoted in breast cancer after transfection of miR-129-5p mimic, lncRNA ZEB1-AS1 siRNA, and ZEB1 siRNA.

Conclusion: In conclusion, the study provides evidence that lncRNA ZEB1-AS1 silencing protects against drug resistance in breast cancer by promoting miR-129-5p-dependent ZEB1 downregulation. It may serve as a novel therapeutic target in breast cancer treatment.
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http://dx.doi.org/10.1186/s12935-020-1164-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092489PMC
March 2020

Diagnosis of Nitrogen Nutrition in Rice Leaves Influenced by Potassium Levels.

Front Plant Sci 2020 27;11:165. Epub 2020 Feb 27.

Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture/Microelement Research Center/College of Resources and Environment, Huazhong Agricultural University, Wuhan, China.

Evaluation of nitrogen (N) status by leaf color is a kind of classic nutritional diagnostic method. However, the color of leaves is influenced not only by N, but also by other nutrients such as potassium (K). Two-year field trials with a factorial combination of N and K were conducted to investigate the effects of different N and K rates on soil plant analysis development (SPAD) readings and leaf N, K, magnesium (Mg), and chlorophyll concentrations. Visual inspections in leaf greenness revealed darker green leaves with increasing N rates, while paler green leaves with increasing K rates. Data showed that SPAD readings, chlorophyll, N and Mg concentrations, and the chloroplast area increased significantly with raising N rates, while declined sharply with the increase in K rates due to the antagonistic relationships between K and NH as well as Mg. It was also probable that the increase in K promoted the growth of leaves and diluted their N and Mg concentrations. The paler leaf appearance resulting from the application of K may overestimate the actual demand for N in the diagnosis of rice N status. The strong antagonistic relationships between K, NH , and Mg should be considered in rice production and fertilization.
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http://dx.doi.org/10.3389/fpls.2020.00165DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7056715PMC
February 2020

Nitrogen fertilization compensation the weak photosynthesis of Oilseed rape (Brassca napus L.) under haze weather.

Sci Rep 2020 03 4;10(1):4047. Epub 2020 Mar 4.

College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, China.

Haze and cloudy weather reduce photo-synthetically active radiation (PAR), which affects the formation of crop yield and nitrogen (N) fertilizer utilization.. We conducted field trails in normal year and severe winter haze year, aiming to compare the difference of photosynthesis and N uptake in winter rapeseed under different N levels. Daily sunshine hours and averaged radiation intensity in winter haze year decreased by 54.1% and 33.3% respectively as compared with the past 30 years. Diurnal variation of net photosynthetic rate in winter haze day was 16.2% lower than that of sunny day. Leaf area and photosynthetic capacity decreased significantly during winter haze year. The shoot biomass and N uptake at the rosette stage accounted for only 9.6% and 26.6% of the total growth period in winter haze year, while 24.4% and 70.5% in normal year, respectively. However, in winter haze year, as the top dressing of N application increasing after the rosette stage, shoot biomass increased gradually. In order to achieve the target yield of 2.5 t ha, after suffering winter haze, it is necessary to apply additional 73.1 kg N ha. In conclusion, the haze climate reduced the radiation intensity and stability, leading to a decline in photosynthetic productivity in winter oilseed rape. Applying higher N fertilizer after winter haze can compensate the negative influence and ensure rapeseed yield.
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http://dx.doi.org/10.1038/s41598-020-60695-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7055290PMC
March 2020

Autophagy of bovine mammary epithelial cell induced by intracellular Staphylococcus aureus.

J Microbiol 2020 Apr 26;58(4):320-329. Epub 2020 Feb 26.

College of Veterinary Medicine, China Agricultural University, Beijing, 100193, P. R. China.

Bovine mastitis is a common disease in the dairy industry that causes great economic losses. As the primary pathogen of contagious mastitis, Staphylococcus aureus (S. aureus) can invade bovine mammary epithelial cells, thus evading immune defenses and resulting in persistent infection. Recently, autophagy has been considered an important mechanism for host cells to clear intracellular pathogens. In the current study, autophagy caused by S. aureus was detected, and the correlation between autophagy and intracellular S. aureus survival was assessed. First, a model of intracellular S. aureus infection was established. Then, the autophagy of MAC-T cells was evaluated by confocal microscopy and western blot. Moreover, the activation of the PI3K-Akt-mTOR and ERK1/2 signaling pathways was determined by western blot. Finally, the relationship between intracellular bacteria and autophagy was analyzed by using autophagy regulators (3-methyladenine [3-MA], rapamycin [Rapa] and chloroquine [CQ]). The results showed that S. aureus caused obvious induction of autophagosome formation, transformation of LC3I/II, and degradation of p62/SQSTM1 in MAC-T cells; furthermore, the PI3K-Akt-mTOR and ERK1/2 signaling pathways were activated. The number of intracellular S. aureus increased significantly with autophagy activation by rapamycin, whereas the number decreased when the autophagy flux was inhibited by chloroquine. Therefore, this study indicated that intracellular S. aureus can induce autophagy and utilize it to survive in bovine mammary epithelial cells.
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http://dx.doi.org/10.1007/s12275-020-9182-8DOI Listing
April 2020
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