Publications by authors named "Jianqiang Chen"

45 Publications

The different biological effects of TMPyP4 and cisplatin in the inflammatory microenvironment of osteosarcoma are attributed to G-quadruplex.

Cell Prolif 2021 Jul 23:e13101. Epub 2021 Jul 23.

Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.

Objective: Osteosarcoma (OS) is characterized by high levels of the tumour-associated inflammatory microenvironment. Moreover, in approximately 60% of OS, telomere length is maintained by alternative lengthening of telomeres (ALT) pathway. Whether the ALT pathway can be exploited for OS therapeutic treatment and how the OS inflammatory microenvironment influences the anti-cancer drug effect remains unknown. Here, we examined the biological effects of TMPyP4 and cisplatin in the inflammatory microenvironment of OS cells.

Materials And Methods: Immunofluorescence in situ hybridization (IF-FISH) and C-circle experiments were used to detect the G-quadruplex and ALT activity. The redox potential of single guanine, G-quadruplex and G-quadruplex/TMPyP4 was evaluated by the lowest unoccupied molecular orbital energy (LUMO), zeta potential and cyclic voltammetry. Cell viability, flow cytometry and apoptosis, Western blot, comet assay, adhesion, transwell and scratch experiments were performed to compare the anti-tumour proliferation and migration effects of TMPyP4 and cisplatin in the inflammatory microenvironment.

Results: This study indicated that compared with cisplatin, TMPyP4 could induce the formation of human telomeres and FAK G-quadruplex in vitro and in vivo, and TMPyP4-treated OS cells showed fewer extrachromosomal C-circles and fewer ALT-associated promyelocytic leukaemia bodies. Consequently, the ALT activity and FAK-related cell migration were suppressed by TMPyP4. Mechanistically, the formation of G-quadruplex resulted in both lower redox potential than G within the genome and FAK transcription inhibition, and TMPyP4 could enhance this phenomenon, especially in the inflammatory microenvironment.

Conclusions: Our results reveal that TMPyP4 is more suitable for OS treatment than cisplatin.
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http://dx.doi.org/10.1111/cpr.13101DOI Listing
July 2021

Eco-friendly utilization of sawdust: Ionic liquid-modified biochar for enhanced Li storage of TiO.

Sci Total Environ 2021 Nov 25;794:148688. Epub 2021 Jun 25.

College of Biology and the Environment, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, PR China. Electronic address:

In China, forestry logging and wood processing produce hundreds of thousands of tons of sawdust every year, which is either discarded or burned. These nonecofriendly practices result in some challenges associated with greenhouse gas emissions. Sawdust-based biochar tailored for anodes of lithium-ion batteries (LIBs) can effectively realize value-added utilization of sawdust. The purpose of the current work is to prepare TiO/biochar nanocomposites to improve the electrical conductivity and structural stability of the anode. However, poor interfacial interaction between TiO and carbon in the TiO/C composites arising from their heterogeneous nature leads to structural deformation of the composites used as anodes of lithium-ion batteries (LIBs). A strategy of constructing ionic liquid-coupled biochar/TiO interfaces is proposed to obtain chemically bonded interfaces between TiO and sawdust-derived biochar. In this study, TiO/C-880 composites are prepared by one-step carbonization of TiO nanoparticles (NPs) and sawdust at 880 °C previously dissolved in 1-butyl-3-methyl-imidazolium ([Bmim]HPO)/dimethyl sulfoxide (DMSO). The morphologies of TiO/C-880 demonstrate that the TiO is encapsulated by porous biochar with intimate interfaces, and the X-ray photoelectron spectroscopy (XPS) results indicate the formation of N-Ti-O/N-O-Ti and Ti-O-P bonds that bridge the two components. TiO/C-880 electrodes have high reversible specific capacities (404 mAh g at 0.1 A g) and desirable long-term cyclic stability (100 mAh g at 2 A g throughout 2500 cycles). Moreover, large diffusion coefficients (D) ranging from 5.9 × 10 to 1.2 × 10 cm s are obtained from galvanostatic intermittent titration (GITT) curves. The N-Ti-O/N-O-Ti and Ti-O-P bonds at the interfaces offer routes for fast Li/electron transport, which account for the high performance of the TiO/C-880 electrodes.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148688DOI Listing
November 2021

Organosolv pretreatment assisted by carbocation scavenger to mitigate surface barrier effect of lignin for improving biomass saccharification and utilization.

Biotechnol Biofuels 2021 Jun 12;14(1):136. Epub 2021 Jun 12.

College of Biology and the Environment, Nanjing Forestry University, No.159 Longpan Road, Nanjing, 210037, China.

Background: Ethanol organosolv (EOS) pretreatment is one of the most efficient methods for boosting biomass saccharification as it can achieve an efficient fractionation of three major constituents in lignocellulose. However, lignin repolymerization often occurs in acid EOS pretreatment, which impairs subsequent enzymatic hydrolysis. This study investigated acid EOS pretreatment assisted by carbocation scavenger (2-naphthol, 2-naphthol-7-sulfonate, mannitol and syringic acid) to improve biomass fractionation, coproduction of fermentable sugars and lignin adsorbents. In addition, surface barrier effect of lignin on cellulose hydrolysis was isolated from unproductive binding effect of lignin, and the analyses of surface chemistry, surface morphology and surface area were carried out to reveal the lignin inhibition mitigating effect of various additives.

Results: Four different additives all helped mitigate lignin inhibition on cellulose hydrolysis in particular diminishing surface barrier effect, among which 2-naphthol-7-sulfonate showed the best performance in improving pretreatment efficacy, while mannitol and syringic acid could serve as novel green additives. Through the addition of 2-naphthol-7-sulfonate, selective lignin removal was increased up to 76%, while cellulose hydrolysis yield was improved by 85%. As a result, 35.78 kg cellulose and 16.63 kg hemicellulose from 100 kg poplar could be released and recovered as fermentable sugars, corresponding to a sugar yield of 78%. Moreover, 22.56 kg ethanol organosolv lignin and 17.53 kg enzymatic hydrolysis residue could be recovered as lignin adsorbents for textile dye removal, with the adsorption capacities of 45.87 and 103.09 mg g, respectively.

Conclusions: Results in this work indicated proper additives could give rise to the form of less repolymerized surface lignin, which would decrease the unproductive binding of cellulase enzymes to surface lignin. Besides, the supplementation of additives (NS, MT and SA) resulted in a simultaneously increased surface area and decreased lignin coverage. All these factors contributed to the diminished surface barrier effect of lignin, thereby improving the ease of enzymatic hydrolysis of cellulose. The biorefinery process based on acidic EOS pretreatment assisted by carbocation scavenger was proved to enable the coproduction of fermentable sugars and lignin adsorbents, allowing the holistic utilization of lignocellulosic biomass for a sustainable biorefinery.
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http://dx.doi.org/10.1186/s13068-021-01988-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199801PMC
June 2021

HO enhances the anticancer activity of TMPyP4 by ROS-mediated mitochondrial dysfunction and DNA damage.

Med Oncol 2021 Apr 21;38(6):59. Epub 2021 Apr 21.

Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, Zhejiang, People's Republic of China.

Cancer is one of the diseases that threatens human health and is a leading cause of mortality worldwide. High levels of reactive oxygen species (ROS) have been observed in cancer tissues compared with normal tissues in vivo, and it is not yet known how this influences chemotherapeutic drug action. Cationic porphyrin 5,10,15,20-tetra-(N-methyl-4-pyridyl) porphyrin (TMPyP4) is a photosensitizer used in photodynamic therapy (PDT) and a telomerase inhibitor used in the treatment of telomerase-positive cancer. Here, we investigated the anticancer activity of TMPyP4 in A549 and PANC cells cultured in HO. The results showed that compared to TMPyP4 alone, the combination of TMPyP4 and HO exhibited sensitization effects on cell viability and colony formation inhibition and apoptosis in A549 and PANC cells, but had no effect in human normal MIHA cells. Mechanistically, the combination of TMPyP4 and HO activates high ROS and mitochondrial membrane potential in A549 and PANC cells, resulting in intense DNA damage and DNA damage responses. Consequently, compared to TMPyP4 alone, TMPyP4 and HO combined treatment upregulates the expression of BAX, cleaved caspase 3, and p-JNK and downregulates the expression of Bcl-2 in A549 and PANC cells. Taken together, these data suggested that HO enhanced the anticancer activity of TMPyP4-mediated ROS-dependent DNA damage and related apoptotic protein regulation, revealing that the high ROS tumor microenvironment plays an important role in chemotherapeutic drug action.
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http://dx.doi.org/10.1007/s12032-021-01505-xDOI Listing
April 2021

Curcumin analogue C66 attenuates obesity-induced renal injury by inhibiting chronic inflammation.

Biomed Pharmacother 2021 May 24;137:111418. Epub 2021 Feb 24.

Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China; School of Pharmaceutical Sciences, Hangzhou Medical College, Hangzhou, Zhejiang 311399, China. Electronic address:

Obesity has been recognized as a major risk factor for the development of chronic kidney disease, which is accompanied by increased renal inflammation, fibrosis, and apoptosis. C66 is a curcumin derivative that exerts anti-inflammatory effects by inhibiting the JNK pathway and prevents diabetic nephropathy. The present study investigates the possible protective effect of C66 on high-fat diet (HFD)-induced obesity-related glomerulopathy. Mice were fed with HFD for 8 weeks while some were treated with C66 every 2 days for 11 weeks. The HFD-fed mice developed renal dysfunction, as well as elevated triglyceride and cholesterol. Kidneys of the HFD-fed mice showed marked glomerular injuries, apoptosis, and inflammation with markedly increased cytokine production. Interestingly, treating HFD-fed mice with C66 remarkably reversed these pathological changes via inhibiting inflammation and NF-κB/JNK activation. In cultured mesangial cells, Palmitic Acid was able to activate the pro-fibrotic mechanisms, apoptosis, inflammatory response, and NF-κB and JNK signaling pathways, all of which could be attenuated by C66 treatment. In all, we demonstrated that curcumin analogue C66 attenuates obesity-induced renal injury by inhibiting chronic inflammation and apoptosis via targeting NF-κB and JNK. Our data suggest that C66 can be potentially used to prevent obesity-associated renal diseases warranting future investigations.
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http://dx.doi.org/10.1016/j.biopha.2021.111418DOI Listing
May 2021

Facile Fabrication of Superhydrophobic Cross-Linked Nanocellulose Aerogels for Oil-Water Separation.

Polymers (Basel) 2021 Feb 19;13(4). Epub 2021 Feb 19.

Institute of Chemical Industry of Forest Products, CAF, Key Lab. of Biomass Energy and Material, Key and Open Lab. of Forest Chemical Engineering, SFA, National Engineering Lab. for Biomass Chemical Utilization, Nanjing 210042, China.

A facile and environmental-friendly approach was developed for the preparation of the cross-linked nanocellulose aerogel through the freeze-drying process and subsequent esterification. The as-prepared aerogel had a three-dimensional cellular microstructure with ultra-low density of 6.05 mg·cm and high porosity (99.61%). After modifying by chemical vapor deposition (CVD) with hexadecyltrimethoxysilane (HTMS), the nanocellulose aerogel displayed stable super-hydrophobicity and super-oleophilicity with water contact angle of 151°, and had excellent adsorption performance for various oil and organic solvents with the adsorption capacity of 77~226 g/g. Even after 30 cycles, the adsorption capacity of the nanocellulose aerogel for chloroform was as high as 170 g/g, indicating its outstanding reusability. Therefore, the superhydrophobic cross-linked nanocellulose aerogel is a promising oil adsorbent for wastewater treatment.
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http://dx.doi.org/10.3390/polym13040625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921982PMC
February 2021

Application of three-dimensional fast gradient echo sequence in observation of craniocervical junctional ligament.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2020 Oct;45(10):1199-1203

Department of Radiology, Haikou Hospital Affiliated to Xiangya School of Medicine, Central South University, Haikou 570208.

Objectives: To explore the value of three-dimensional fast gradient echo sequence (3D-GRE) in observation of the craniocervical junctional ligament.

Methods: A total of 21 healthy volunteers underwent 3D-GRE imaging. The imaging data was imported into the post-processing workstation. The structures of the ligaments in the craniocervical junctional area were observed and evaluated by multiplanar reconstruction technique.

Results: The features of ligaments in the craniocervical junction were shown clearly for all the 21 cases of volunteers. The scan time was 267-294 s. After the treatment with the three-dimensional reconstruction technique, the signal characteristics and the running structure of the transverse ligament, the alar ligament, the serrated ligament and the lamina could be effectively displayed.

Conclusions: The 3D-GRE can evaluate the three-dimensional data of craniocervical junctional ligament in a short period of time. Post-processing reconstruction technique can clearly evaluate the structure characteristics of each ligament, which can lay a foundation for further application in craniocerebral trauma patients.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2020.190271DOI Listing
October 2020

AQP4-siRNA alleviates traumatic brain edema by altering post-traumatic AQP4 polarity reversal in TBI rats.

J Clin Neurosci 2020 Nov 5;81:113-119. Epub 2020 Oct 5.

Department of Radiology, Xiangya School of Medicine Affiliated Haikou Hospital, Central South University, Haikou, Hainan 570208, China. Electronic address:

The spatial and temporal distribution of aquaporin-4 (AQP4) expression in rat brain following brain trauma and AQP4-siRNA treatment, as well as corresponding pathological changes, were studied to explore the mechanism underlying the effect of AQP4-siRNA treatment on traumatic brain injury (TBI). The rats in the sham operation group had normal structure, with AQP4 located in the perivascular end-foot membranes and astrocytic membranes in a polarized pattern. The accelerated polarity reversal was observed in the TBI group in 1-12 h after TBI. During this period, AQP4 abundance on the astrocytic membrane is gradually increased, while AQP4 abundance on the perivascular end-foot membrane declined rapidly. Twelve hours after TBI, AQP4 expression was depolarized, showing a shift from the perivascular end-foot membrane to the astrocytic membrane. Pathological observation showed that vasogenic edema occurred immediately after TBI, at which time the extracellular space was expanded, leading to severe intracellular edema. AQP4-siRNA reduced the polarity reversal index at the early stage of TBI recovery and reduced edema, demonstrating the potential benefit of reduced AQP4 expression during recovery from TBI.
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http://dx.doi.org/10.1016/j.jocn.2020.09.015DOI Listing
November 2020

Synthesis of biocompatible and highly fluorescent N-doped silicon quantum dots from wheat straw and ionic liquids for heavy metal detection and cell imaging.

Sci Total Environ 2021 Apr 5;765:142754. Epub 2020 Oct 5.

College of Biology and the Environment, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, PR China.

Silane-based precursors for the synthesis of water-dispersible silicon quantum dots (SiQDs) present harmful effects on both researchers and the environment, due to their high toxicity. Though waste wheat straw is an abundant source of natural silicon, its application towards the synthesis of biocompatible SiQDs for metal detection has not yet been explored. In this study, N-doped SiQDs demonstrating uniform spherical morphologies, excellent water dispersity and strong fluorescence emission with a quantum yield of 28.9% were facilely synthesized by using wheat straw (WS) as silicon source and allyl-3-methylimidazolium chloride (AMIMCl) as nitrogen source. The wheat straw based SiQDs (WS-SiQDs) showed linear fluorescence quenching ((F-F)/F) with Cr(VI) and Fe(III) concentration in the range of 0-6 × 10 M. Following immobilization on hydrophilic silica hydrogels, [email protected] hydrogels demonstrated enhanced fluorescence emission which can selectively detect Cr(VI) and Fe (III) to the limits of 142 and 175 nM, respectively. Moreover, cell imaging results reflected that WS-SiQDs can penetrate the membranes of dental pulp stem cells and react with the nucleuses of the stem cells. The stem cells maintained high viability under the conditions of 24 h incubation and SiQD concentration below 50 mg·L, thus indicating low cytotoxicity of WS-SiQDs. The as-prepared SiQDs demonstrated notable structural and fluorescent properties, therefore representing promising biocompatible fluorescent nanomaterials for metal detection and cell imaging.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142754DOI Listing
April 2021

Clinical features of new-onset systemic lupus erythematosus during pregnancy in Central China: a retrospective study of 68 pregnancies.

Clin Rheumatol 2021 Jun 16;40(6):2121-2131. Epub 2020 Oct 16.

Department of Rheumatology, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Road, Zhengzhou, Henan Province, China.

The aim of this study is to explore the clinical features and pregnancy outcomes of Chinese patients with new-onset systemic lupus erythematosus (SLE) during pregnancy or puerperium. We retrospectively evaluated the data of all pregnant women with SLE managed at the First Affiliated Hospital of Zhengzhou University between April 2013 and March 2017. Clinical characteristics, laboratory features, medication use, and pregnancy outcomes were compared between pregnant women with new-onset SLE and pregnant women with pre-existing SLE. Risk factors for adverse pregnancy outcomes were determined using binary regression analyses. Overall, 223 pregnancies in 216 patients were included; 148 (69.6%) patients had a history of SLE, and 68 (30.4%) were diagnosed with SLE during pregnancy or puerperium. Most cases of new-onset SLE (72.1%) occurred during the first and second trimesters. Thrombocytopaenia (especially severe thrombocytopaenia) (76.5% vs 54.2%, P = 0.008; 39.7% vs 15.5%, P = 0.001) and anaemia (especially moderate anaemia) (73.5% vs 56.9%, P = 0.007; 52.9% vs 35.2%, P = 0.035) were more common in women with new-onset SLE than in women with pre-existing SLE and active disease during pregnancy. Additionally, patients with new-onset SLE experienced higher rates of moderate-to-severe disease activity than patients with pre-existing SLE (P < 0.01); disease activity occurred mostly during the first and second trimesters (75.4%). Compared with pre-existing SLE patients, disease activity in new-onset SLE patients occurred mostly in the first trimester (33.3% vs 15.3%, P = 0.043) and less in the third trimester (21.1% vs 47.2%, P < 0.001). Pregnancy loss was significantly higher in patients with new-onset SLE than in patients with pre-existing SLE (62.4% vs 27.1%, P < 0.001), with most cases occurring during the first and second trimesters (95.3%). However, there were no significant differences in neonatal outcomes between new-onset and pre-existing SLE patients with active disease. Within the new-onset SLE group, active disease was an independent risk factor for pregnancy loss (odds ratio [OR] = 16.185, confidence interval [CI] = 1.895-138.232, P = 0.011), whereas disease onset at late gestation was a protective factor against pregnancy loss (OR = 0.589, CI = 0.435-0.796, P = 0.013). Patients with new-onset SLE suffered greater haematological involvement (mainly thrombocytopaenia and anaemia) and higher rates of moderate-to-severe disease activity and pregnancy loss than patients with pre-existing SLE. Controlling disease activity and extending gestational age may improve pregnancy outcomes in women with new-onset SLE. Key Points • The clinical features of new-onset SLE during pregnancy and its impact on pregnancy outcomes have rarely been reported, especially in Chinese patients. • New-onset SLE during pregnancy in Chinese women occurred primarily during the first and second trimesters and was characterised by haematological disorders, including thrombocytopaenia and anaemia. • Women with new-onset SLE during pregnancy had significantly higher disease activity scores and pregnancy loss rates than women with pre-existing SLE, especially during the first and second trimesters; controlling disease activity and prolonging gestational age may improve pregnancy outcomes in this setting.
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http://dx.doi.org/10.1007/s10067-020-05452-2DOI Listing
June 2021

RTK/Pseudolite/LAHDE/IMU-PDR Integrated Pedestrian Navigation System for Urban and Indoor Environments.

Sensors (Basel) 2020 Mar 24;20(6). Epub 2020 Mar 24.

State Key Laboratory of satellite navigation system and equipment technology, 589 ZhongShan Street, Qiaoxi District Shijiazhuang 050081, China.

This paper presents an evaluation of real-time kinematic (RTK)/Pseudolite/landmarks assistance heuristic drift elimination (LAHDE)/inertial measurement unit-based personal dead reckoning systems (IMU-PDR) integrated pedestrian navigation system for urban and indoor environments. Real-time kinematic (RTK) technique is widely used for high-precision positioning and can provide periodic correction to inertial measurement unit (IMU)-based personal dead reckoning systems (PDR) outdoors. However, indoors, where global positioning system (GPS) signals are not available, RTK fails to achieve high-precision positioning. Pseudolite can provide satellite-like navigation signals for user receivers to achieve positioning in indoor environments. However, there are some problems in pseudolite positioning field, such as complex multipath effect in indoor environments and integer ambiguity of carrier phase. In order to avoid the limitation of these factors, a local search method based on carrier phase difference with the assistance of IMU-PDR is proposed in this paper, which can achieve higher positioning accuracy. Besides, heuristic drift elimination algorithm with the assistance of manmade landmarks (LAHDE) is introduced to eliminate the accumulated error in headings derived by IMU-PDR in indoor corridors. An algorithm verification system was developed to carry out real experiments in a cooperation scene. Results show that, although the proposed pedestrian navigation system has to use human behavior to switch the positioning algorithm according to different scenarios, it is still effective in controlling the IMU-PDR drift error in multiscenarios including outdoor, indoor corridor, and indoor room for different people.
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http://dx.doi.org/10.3390/s20061791DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7147164PMC
March 2020

Monitoring graphene oxide's efficiency for removing Re(VII) and Cr(VI) with fluorescent silica hydrogels.

Environ Pollut 2020 Jul 24;262:114246. Epub 2020 Feb 24.

Department of Environmental Engineering, College of Biology and the Environment, Nanjing Forestry University, 159 Longpan Road, Nanjing, 210037, PR China. Electronic address:

Supported carbon quantum dots (CQDs), used as fluorescent sensors for the detection of metal ions, have rarely been used to remove heavy metals from water. Nitrogen-doped CQDs immobilized in hydrophilic silica hydrogels exhibited a more superior sensitivity and selectivity for the detection of Re(VII) and Cr(VI) than other metal ions, including Fe(III), Fe(II), Zn(II), Cu(II) and Mn(II). For the first time, low limits of detection (LOD) of 2.3 μM for Re(VII) detection and 65 nM for Cr(VI) detection were reported by a facile method. Based on the high selectivity of fluorescent silica hydrogels for Re(VII) and Cr(VI) detection, the removal of Re(VII) and Cr(VI) by graphene oxide (GO) in water was monitored with the hydrogels used as a turn-off fluorescent sensing platform. The consistent results of the sorption isotherms of each metal on GO, which were obtained from the fluorescence spectra and by UV absorption, further verified the possibility of monitoring metal removal by fluorescence detection. Remarkably, GO removed 1186 mg/g of Re(VII) but only 178 mg/g of Cr(VI). The density functional theory (DFT) calculations indicated that both Re(VII) and Cr(VI) formed stable bonds with silica hydrogels, confirming that the interactions between the metal ions and the substrate would promote the fluorescence quenching of the supported CQDs. On the other hand, Re(VII) interacted more strongly with the carboxyl groups of GO than Cr(VI). In addition, a real-time detection system was designed to alarm the service life of a GO filter used for Re(VII) removal.
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http://dx.doi.org/10.1016/j.envpol.2020.114246DOI Listing
July 2020

Effect of AQP4-RNAi in treating traumatic brain edema: Multi-modal MRI and histopathological changes of early stage edema in a rat model.

Exp Ther Med 2020 Mar 15;19(3):2029-2036. Epub 2020 Jan 15.

Department of Radiology, The Seventh People's Hospital of Chongqing, Chongqing 400054, P.R. China.

Traumatic brain injury (TBI) is one of the leading causes of mortality and permanent disabilities worldwide. Brain edema following TBI remains to be the predominant cause of mortality and disability in patients worldwide. Previous studies have reported that brain edema is closely associated with aquaporin-4 (AQP4) expression. AQP4 is a water channel protein and mediates water homeostasis in a variety of brain disorders. In the current study, a rat TBI model was established, and the features of brain edema following TBI were assessed using multimodal MRI. The results of the multimodal MRI were useful, reliable and were used to evaluate the extent and the type of brain edema following TBI. Brain edema was also successfully alleviated using an intracerebral injection of AQP4 small interfering (si)RNA. The expression of AQP4 and its role in brain edema were also examined in the present study. The AQP4 siRNA was demonstrated to downregulate AQP4 expression following TBI and reduced brain edema at the early stages of TBI (6 and 12 h). The current study revealed the MRI features of brain edema and the changes in AQP4 expression exhibited following TBI, and the results provide important information that can be used to improve the early diagnosis and treatment of brain edema.
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http://dx.doi.org/10.3892/etm.2020.8456DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7027281PMC
March 2020

Radiomics model of magnetic resonance imaging for predicting pathological grading and lymph node metastases of extrahepatic cholangiocarcinoma.

Cancer Lett 2020 02 3;470:1-7. Epub 2019 Dec 3.

Department of Radiology, The Affiliated Hospital of Southwest Medical University, 25 Taiping Street, Luzhou, 646000, Sichuan, China. Electronic address:

The aim of this study was to evaluate diagnostic performance of radiomics models of MRI in the detection of differentiation degree (DD) and lymph node metastases (LNM) of extrahepatic cholangiocarcinoma (ECC). We retrospectively enrolled 100 patients with ECC confirmed by pathology from January 2011 to December 2018. Three hundred radiomics features were extracted from each region of interest using MaZda software. Next, the radiomics model was developed by incorporating the optimal radiomics signatures and ADC values of tumors to predict DD (model A) and LNM (model B) of ECC, respectively, through the random forest algorithm. After which, the performance of the radiomics models were further evaluated. The model A showed better performance in both training and testing cohorts to discriminate high and medium-low differentiation groups of ECC, with an average AUC of 0.78 and 0.80, respectively. The model B also yielded the good average AUC of 0.80 and 0.90 to predict the LNM of ECC in training and testing cohorts. The radiomics models based on MRI performed well in predicting DD and LNM of ECC and have significant potential in clinical noninvasive diagnosis and in the prediction of ECC.
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http://dx.doi.org/10.1016/j.canlet.2019.11.036DOI Listing
February 2020

Carbogen gas-challenge BOLD fMRI in assessment of liver hypoxia after portal microcapsules implantation.

PLoS One 2019 27;14(11):e0225665. Epub 2019 Nov 27.

Department of Radiology, Xiangya School of Medicine Affiliated Haikou Hospital, Central South University, Haikou, Hainan, China.

Background: Hypoxia is one of the key factors affecting the survival of islet cells transplanted via the portal vein. Blood oxygen level dependent functional magnetic resonance imaging (BOLD-fMRI) is the only imaging technique that can detect the level of blood oxygen level in vivo. However, so far no study has indicated that BOLD-fMRI can be applied to monitor the liver oxygen level after islet transplantation.

Objective: To evaluate the value of Carbogen-challenge BOLD MRI in assessing the level of hypoxia in liver tissue after portal microcapsules implanted.

Methods: Fifty-one New Zealand rabbits were randomly divided into three experimental groups (15 in each group) were transplanted microencapsulated 1000 microbeads/kg (PV1 group), 3000 microbeads/kg (PV2 group), 5000 microbeads/kg (PV3 group), and 6 rabbits were injected with the same amount of saline as the control group, BOLD-fMRI was performed following carbogen breathing in each group after transplantation on 1d, 2d, 3d and 7d, T2* weighted image, R2* value and ΔR2* value parameters for the liver tissue. Pathological examinations including liver gross pathology, H&E staining and pimonidazole immunohistochemistry were performed after BOLD-fMRI. The differences of pathological results among each group were compared. The ΔR2* values and transplanted doses were analyzed.

Results And Conclusions: ΔR2* values at the 1-3d and 7d after transplantation were significantly different in each groups (P<0.05). ΔR2* values decreased gradually with the increase of transplanted dose, and was negatively correlated with transplant dose at 3d after transplantation (r = -0.929, P <0.001). Liver histopathological examination showed that the degree of hypoxia of liver tissue increased with the increase of transplanted doses, Carbogen-challenge BOLD-fMRI can assess the degree of liver hypoxia after portal microcapsules implanted, which provided a monitoring method for early intervention.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0225665PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6881018PMC
March 2020

Secreting-lux/pT-ClyA engineered bacteria suppresses tumor growth via interleukin-1β in two pathways.

AMB Express 2019 Nov 21;9(1):189. Epub 2019 Nov 21.

Department of Radiology, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, No. 43, Renmin Avenue, Haikou, 570208, Hainan, China.

Engineered Salmonella typhimurium (S.t-ΔpG) and attenuated Salmonella typhimurium (SL: Salmonella typhimurium with a defect in the synthesis of guanine 5'-diphosphate-3'-diphosphate) exhibit similar tumor targeting capabilities (Kim et al. in Theranostics 5:1328-1342, 2015; Jiang et al. in Mol Ther 18:635-642, 2013), but S.t-ΔpG exerts superior tumor suppressive effects. The aim of this study was to investigate whether S.t-ΔpG inhibits colon cancer growth and recurrence by promoting increased IL-1β production. The CT26 tumor mouse model was used, and mice were treated in the following ways: PBS, S.t-ΔpG + IL-1βAb, SL, S.t-ΔpG, and S.t-ΔpG. Dynamic evaluation of the efficacy of S.t-ΔpG in the treatment of colon cancer was assessed by MRI. Western blot, immunofluorescence and flow cytometry analysis were used to investigate IL-1β-derived cells and IL-1β expression on tumor cells and immune cells to analyze the regulatory mechanism. IL-1β levels in tumors colonized by S.t-ΔpG were significantly increased and maintained at high levels compared to control treatments. This increase caused tumors to subside without recurrence. We examined the immune cells mediating S.t-ΔpG-induced tumor suppression and examined the major cell types producing IL-1β. We found that macrophages and dendritic cells were the primary IL-1β producers. Inhibition of IL-1β in mice treated with S.t-ΔpG using an IL-1β antibody caused tumor growth to resume. This suggests that IL-1β plays an important role in the treatment of cancer by S.t-ΔpG. We found that in St-ΔpG-treated tumors, expression of molecules involved in signaling pathways, such as NLRP3, ASC, Caspase1, TLR4, MyD88, NF-kB and IL-1β, were upregulated, while in ΔppGpp S. typhimurium treated animals, TLR4, MyD88, NF-kB and IL-1β were upregulated with NLRP3, ASC, and Caspase1 being rarely expressed or not expressed at all. Using S.t-ΔpG may simultaneously activate TLR4 and NLRP3 signaling pathways, which increase IL-1β expression and enhance inhibition of colon cancer growth without tumor recurrence. This study provides a novel platform for treating colon cancer.
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http://dx.doi.org/10.1186/s13568-019-0910-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6872689PMC
November 2019

Dendrobium officinale polysaccharides alleviate colon tumorigenesis via restoring intestinal barrier function and enhancing anti-tumor immune response.

Pharmacol Res 2019 10 29;148:104417. Epub 2019 Aug 29.

Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510006, Guangdong, PR China; School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, Guangdong, PR China. Electronic address:

Intact epithelial barrier and mucosal immune system are crucial for maintaining intestinal homeostasis. Previous study indicated that Dendrobium officinale polysaccharides (DOPS) can regulate immune responses and inflammation to alleviate experimental colitis. However, it remains largely unknown whether DOPS can suppress AOM/DSS-induced colorectal cancer (CRC) model through its direct impact on intestinal barrier function and intestinal mucosal immunity. Here, we demonstrated the therapeutic action of DOPS for CRC model and further illustrated its underlying mechanisms. Treatment with 5-aminosalicylic acid (5-ASA) and DOPS significantly improved the clinical signs and symptoms of chronic colitis, relieve colon damage, suppress the formation and growth of colon tumor in CRC mice. Moreover, administration of DOPS effectively preserved the intestinal barrier function via reducing the loss of zonula occludens-1 (ZO-1) and occludin in adjacent tissues and carcinomatous tissues. Further studies demonstrated that DOPS improved the metabolic ability of tumor infiltrated CD8 cytotoxic T lymphocytes (CTLs) and reduced the expression of PD-1 on CTLs to enhance the anti-tumor immune response in the tumor microenvironments (TME). Together, the conclusions indicated that DOPS restore intestinal barrier function and enhance intestinal anti-tumor immune response to suppress CRC, which may be a novel strategy for the prevention and treatment of CRC.
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http://dx.doi.org/10.1016/j.phrs.2019.104417DOI Listing
October 2019

Silibinin restores the sensitivity of cisplatin and taxol in A2780-resistant cell and reduces drug-induced hepatotoxicity.

Cancer Manag Res 2019 26;11:7111-7122. Epub 2019 Jul 26.

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, People's Republic of China.

Purpose: Ovarian cancer is the most lethal cancer among all gynaecological malignancies. The combination theraputics of cisplatin and taxol is widely used in clinicals for ovarian cancer treatment. However, long-term use of cisplatin and taxol induces strong tolerance and hepatotoxicity. Since silibinin is a commonly used anti-hepatotoxic drug in Europe and Asia, the aim of this study was to determine whether silibinin could restore the sensitivity of combination use of cisplatin and taxol in drug-resistant human ovarian cancer cells and reduce drug-induced hepatotoxicity.

Patients And Methods: Normal hepatocyte LO2 cells and A2780/DDP cells were treated with silibinin, cisplatin, taxol, cisplatin and taxol plus silibinin for 48 h. Cell viability was determined by MTT and long-term proliferation assay, while apoptosis and cell cycle progression were assessed by flow cytometric analysis. DNA damage was evluated by immunofluorescence assays. The metastatic activity of A2780/DDP was determined by cell adhesion assay.

Results: The addition of silibinin on cisplatin and/or toxal could sensitize the antitumor activity of cisplatin and toxal on A2780/DDP cells, supress cell-matrix adhesion of A2780/DDP, inhibit the cell proliferation, result in A2780/DDP cells apoptosis. In addition, silibinin could effectively reduce cisplatin and/or toxal-induced hepatotoxicity by protecting DNA from damage and restoring the potential of cell proliferation in cisplatin and/or toxal-treated LO2 cells.

Conclusion: Our results suggest that silibinin could restore the sensitivity of cisplatin and taxol in drug-resistant human ovarian cancer cells and reduce durg-induced hepatotoxicity in cell level.
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http://dx.doi.org/10.2147/CMAR.S201341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6668021PMC
July 2019

Mechanochemical esterification of waste mulberry wood by wet Ball-milling with tetrabutylammonium fluoride.

Bioresour Technol 2019 Aug 16;285:121354. Epub 2019 Apr 16.

Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Key Lab of Biomass Energy and Material, Jiangsu Province, National Engineering Lab for Biomass Chemical Utilization, Key Lab on Forest Chemical Engineering, State Forestry Administration, Nanjing 210042, PR China. Electronic address:

Esterification of lignocellulosic biomass driven by dry ball-milling suffered from agglomeration of lignocellulosic matters during milling process. In this study, esterification of waste mulberry wood (MW) was carried out by wet ball-milling with water and tetrabutylammonium fluoride (TBAF) to prepare all-wood-plastic composites. Under the same condition, the esterification of MW by wet ball-milling with TBAF presented higher efficiency than that without TBAF which was attributed to catalytic function of F ions meanwhile the binding of TBA to cellulose fibrils hindered the compaction of fibrillated fragments. Pre-ball-milling of MW for 4.0 h apparently promoted the esterification with succinic anhydride. All-wood-plastic composites prepared after 7.0 h succinoylation demonstrated prominent mechanical performance due to strong adhesion of fragments and matrix. This study is supposed to provide an environment-friendly method for efficient conversion of waste lignocellulosic biomass.
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http://dx.doi.org/10.1016/j.biortech.2019.121354DOI Listing
August 2019

Effects of nanocellulose on sodium alginate/polyacrylamide hydrogel: Mechanical properties and adsorption-desorption capacities.

Carbohydr Polym 2019 Feb 30;206:289-301. Epub 2018 Oct 30.

College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, Jiangsu, China; Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing 210042, Jiangsu, China.

In this study, interpenetrating polymer network (IPN)-structured hydrogels were fabricated through the crosslinking of neutral polyacrylamide (PAM, the first flexible network) and the polyelectrolyte sodium alginate (SA, the second rigid network). Three types of nanocellulose, including cellulose nanocrystals (CNCs), bacterial cellulose fibers (BCs) and 2,2,6,6-tetramethylpiperidine-1-oxy radical (TEMPO)-oxidized cellulose nanofibers (TOCNs), were well-dispersed in the SA-PAM gel matrix. The hydrogels, obtained with a high water content (∼83%), exhibit a macroporous structure with a mean pore size of 60 ± 51 μm and high transparency. Comparative studies indicate that BCs are more effective for enhancing the hydrogels due to their higher aspect ratio, and the compressive strength of SA-PAM-BC hydrogel is 6.59 times higher than that of neat SA-PAM. Meanwhile, TOCNs are the best fillers for improving the adsorption capacity owing to the presence of a carboxyl group, and the adsorption capacity of SA-PAM-TOCN is more than 1.3-fold that in SA-PAM. Prepared hydrogels with high mechanical strength and adsorption capacity are advantageous for their applications in wastewater treatments.
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http://dx.doi.org/10.1016/j.carbpol.2018.10.105DOI Listing
February 2019

Effects of dexamethasone on C6 cell proliferation, migration and invasion through the upregulation of AQP1.

Oncol Lett 2018 May 15;15(5):7595-7602. Epub 2018 Mar 15.

Department of Radiology, The Seventh People's Hospital of Chongqing, Chongqing 400054, P.R. China.

Dexamethasone (Dex) is commonly used to treat glioma; however, the mechanism underlying the action of Dex remains unclear. In the present study, the hypothesis that aquaporin-1 (AQP1) may participate in tumor cell proliferation, apoptosis, migration and invasion was tested using small interfering RNA (siRNA). The results of the current study indicated that Dex could inhibit the proliferation, in addition to promoting the migration, of C6 cells. Dex was indicated to promote the expression of AQP1. Downregulation of AQP1, achieved using siRNAs, demonstrated the inhibition of cell proliferation, promotion of cell migration and suppression of invasion; therefore, Dex was indicated to serve a role in these effects in the C6 cells, via the upregulation of AQP1. This demonstrated that AQP1 could be utilized as a novel therapeutic target, with the aim of inhibiting the proliferation and metastasis of gliomas.
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http://dx.doi.org/10.3892/ol.2018.8269DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5934719PMC
May 2018

Biofilter treatment of gas phase β-caryophyllene at an elevated temperature.

J Environ Sci Health A Tox Hazard Subst Environ Eng 2018 Jul 19;53(8):752-765. Epub 2018 Mar 19.

d College of Material Science and Engineering , Nanjing Forestry University , Nanjing , Jiangsu , China.

Experiments were carried out to test the capacity for a laboratory-scale biofilter operated at an elevated temperature level (∼50°C) to remove an air stream containing β-caryophyllene, a naturally occurring sesquiterpene of environmental concern emitted from wood-related industrial facilities. A water jacket was used to maintain high temperatures in a laboratory-scale biofilter. Inocula, pollutant loading and nutrient supply rate effects were evaluated over 84 days of biofilter operation. The start-up process took over two months when citrus peels were used as inocula while a relatively short start-up period was achieved after introducing forest compost products. While using a sparged-gas bioreactor to cultivate an enrichment culture for 97 days, removal efficiencies in excess of 80% were observed after 18 days. At empty bed contact times of 50 s and at a pollutant loading rate of 3.05 mg C/L/hr, removal efficiency levels reached 90% and the elimination capacity level reached 2.29 mg C/L/hr, corresponding to an elimination capacity of 2.60 mg β-caryophyllene/L/hr. Collectively, these results demonstrate that β-caryophyllene can be successfully removed from contaminated air using a biofilter operated at a high temperature (∼50°C), expanding the temperature range within which biofilters are known to biodegrade sesquiterpenes.
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http://dx.doi.org/10.1080/10934529.2018.1444969DOI Listing
July 2018

Thermally Tunable Pickering Emulsions Stabilized by Carbon-Dot-Incorporated Core-Shell Nanospheres with Fluorescence "On-Off" Behavior.

Langmuir 2018 01 21;34(1):273-283. Epub 2017 Dec 21.

Department of Agro-Environmental Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University , 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.

Lack of deep understanding of nanoparticle (NP) actions at oil/water interface set an obstacle to practical applications of Pickering emulsions. Fluorescence labels fabricated by incorporation of carbon dots (CDs) into poly(N-isopropylacrylamide) (PNIPAM) matrix can not only mark the action of PNIPAM-based NPs in the interface but also reflect the colloidal morphologies of PNIPAM. In this work, we employed coaxial electrospraying for fabricating core-shell nanospheres of cellulose acetate encapsulated by PNIPAM, and facile incorporation of CDs in PNIPAM shells was achieved simultaneously. The coaxial electrosprayed NPs (CENPs) with temperature-dependent wettability can stabilize heptane and toluene in water at 25 °C, respectively, and reversible emulsion break can be triggered by temperature adjustment around the low critical solution temperature (LCST). Remarkably, CENP/CD composites exhibited a fluorescence "on-off" behavior because of the volume phase transition of the PNIPAM shell. CENP/CD composites in Pickering emulsions clearly elucidated the motions of CENPs in response to temperature changes. At temperatures below the LCST, the CENP concentration played an important role in surface coverage of oil droplets. Specifically, the CENP concentration above the minimum concentration for complete emulsification of oil phase led to high surface coverage and two-domain adsorption of CENPs at the interface including primary monolayer anchoring of CENPs on droplets surrounded by interconnected CENP networks, which contributed to the superior stability of the emulsions. Moreover, CENP/CD composites can be recycled with well-preserved core-shell structure and stable fluorescent properties, which offers their great potential applications in sensors and imaging.
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http://dx.doi.org/10.1021/acs.langmuir.7b03490DOI Listing
January 2018

The Diagnostic Accuracy of Conventional Forceps Biopsy Compared to ESD.

Open Med (Wars) 2017 12;12:261-265. Epub 2017 Aug 12.

Department of Gastroenterology, the First Affiliated Hospital, College of Medicine, Zhejiang University, No.79 Qingchun Road, Hangzhou310003, China.

Objective: conventional forceps biopsy (CFB) is the most popular way to screen for gastric epithelial neoplasia (GEN). Our study aimed to compare the diagnostic accuracy between conventional forceps biopsy and endoscopic submucosal dissection (ESD).

Methods: 105 patients diagnosed GEN finally undertook ESD in our hospital were enrolled. We retrospectively assessed the characteristics of pathological results of CFB and ESD.

Results: The overall pathologic concordance rate between the CFB and ESD specimens was 68.57%. 55 cases of CFB maintained low-grade intraepithelial neoplasia (LGIN) under ESD,18 cases (23.1%) diagnosis for high-grade intraepithelial neoplasia (HGIN), 5 cases (6.4%) diagnosis for cancer. Moreover, 10 cases of CFB maintained HGIN under ESD. Lesions with surface hyperemia (44.4% vs. 27.54%) or surface ulcer (57.14% vs.26.76%) were more likely to cancerate (P<0.05).

Conclusion: endoscopic biopsy in the diagnosis of low-grade intraepithelial neoplasia, may exist or progression to high-grade intraepithelial neoplasia, some may have cancer, should take active treatment measures.
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http://dx.doi.org/10.1515/med-2017-0039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5558105PMC
August 2017

[Correlation between the mRNA expression of tissue inhibitor of metalloproteinase-1 and apparent diffusion coefficient on diffusion-weighted imaging in rats' liver fibrosis].

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2017 Feb;42(2):161-167

Department of Radiology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.

Objective: To explore the correlation between the apparent diffusion coefficient (ADC) and mRNA expression of tissue inhibitor of metalloproteinase-1 (TIMP-1) in different stages of liver fibrosis in rats.
 Methods: A model of liver fibrosis in rats was established by intraperitoneal injection of high-fat diet combined with porcine serum. After drug administration for 4 weeks, 48 rats served as a model group and 12 rats served as a control group, then they underwent diffusion weighted imaging (DWI) scanning. The value of ADC was calculated at b value=800 s/mm2. The rats were sacrificed and carried out pathologic examination after DWI scanning immediately. The mRNA expression of TIMP-1 was detected by real time-polymerase chain reaction (RT-PCR). The rats of hepatic fibrosis were also divided into a S0 group (n=4), a S1 group (n=11), a S2 group (n=12), a S3 group (n=10), and a S4 group (n=9) according to their pathological stage. The value of ADC and the expression of TIMP-1 mRNA among the different stage groups of liver fibrosis were compared, and the correlation between ADC and the TIMP-1 mRNA were analyzed.
 Results: The ADC value and the TIMP-1 mRNA expression were significantly different between the control group and the liver fibrosis group (F=46.54 and 53.87, P<0.05). There were significant differences in the value of ADC between every two groups (all P<0.05), except the control group vs the S1 group, the S1 group vs the S2 group, and the S2 group vs the S3 group (all P>0.05). For the comparison of TIMP-1 mRNA, there was no significant difference between the S1 group and the S2 group, the S3 group and the S4 group (both P>0.05). There were significant differences among the rest of the groups (all P<0.05). Rank correlation analysis showed that there was a negative correlation between the ADC value and the TIMP-1 mRNA expression (r=-0.76, P<0.01).
 Conclusion: When the value of ADC decreases in the progress of rats' liver fibrosis, the mRNA expression of TIMP-1 increases gradually, and there is a negative correlation between them.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2017.02.007DOI Listing
February 2017

Separation and Sequential Recovery of Tetracycline and Cu(II) from Water Using Reusable Thermoresponsive Chitosan-Based Flocculant.

ACS Appl Mater Interfaces 2017 Mar 8;9(11):10266-10275. Epub 2017 Mar 8.

College of Biology and the Environment, Nanjing Forestry University , Nanjing 210037, P. R. China.

Coexistence of antibiotics and heavy metals is typically detected in water containing both organic and inorganic contaminants. In this work, a flocculation method using a reusable thermoresponsive chitosan-based flocculant (CS-g-PNNPAM) was applied for separation and sequential recovery of tetracycline (TC) and Cu(II) from water. High synergistic removal rates of both TC and Cu(II) from water (>90%) were reached. Interactive effects among targeted water temperature (T), stock solution temperature (T), and flocculant dosage on flocculation performance were assessed using response surface methodology. To optimize flocculation, operation strategies of adjusting T and dosage according to T based on the interactive effects were given through mathematical analyses. The flocculation mechanism as well as interfacial interactions among CS-g-PNNPAM, TC, and Cu(II) were studied through experimental investigations (floc size monitoring, X-ray photoelectron spectroscopy, and UV spectra) and theoretical calculations (density functional theory and molecular dynamics simulations). Coordination of Cu(II) with TC and the flocculant promoted flocculation; switchable interactions (H bonds and hydrophobic association) of the TC-flocculant at different temperatures were key factors affecting operation strategies. When these interactions were weakened step by step, TC and Cu(II) were sequentially recovered from flocs using certain solutions. Meanwhile, the flocculant in flocs was regenerated and found reusable with high flocculation efficiency.
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http://dx.doi.org/10.1021/acsami.7b00828DOI Listing
March 2017

Origin and Quenching of Novel ultraviolet and blue emission in NdGaO3: Concept of Super-Hydrogenic Dopants.

Sci Rep 2016 11 3;6:36352. Epub 2016 Nov 3.

NUSNNI-Nanocore, National University of Singapore, 117411, Singapore.

In this study we report the existence of novel ultraviolet (UV) and blue emission in rare-earth based perovskite NdGaO (NGO) and the systematic quench of the NGO photoluminescence (PL) by Ce doping. Study of room temperature PL was performed in both single-crystal and polycrystalline NGO (substrates and pellets) respectively. Several NGO pellets were prepared with varying Ce concentration and their room temperature PL was studied using 325 nm laser. It was found that the PL intensity shows a systematic quench with increasing Ce concentration. XPS measurements indicated that nearly 50% of Ce atoms are in the 4+ state. The PL quench was attributed to the novel concept of super hydrogenic dopant (SHD)", where each Ce ion contributes an electron which forms a super hydrogenic atom with an enhanced Bohr radius, due to the large dielectric constant of the host. Based on the critical Ce concentration for complete quenching this SHD radius was estimated to be within a range of 0.85 nm and 1.15 nm whereas the predicted theoretical value of SHD radius for NdGaO3 is ~1.01 nm.
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http://dx.doi.org/10.1038/srep36352DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5093437PMC
November 2016

Prognostic impact of serum and tissue MMP-9 in non-small cell lung cancer: a systematic review and meta-analysis.

Oncotarget 2016 Apr;7(14):18458-68

Department of General Surgery, Zhejiang Provincial People's Hospital, Hangzhou 310004, China.

Matrix metalloproteinases-9 (MMP-9) was one of the most important enzyme to breakdown extracellular matrix, aim to clarify the prognostic value of MMP-9 in non-small cell lung cancer (NSCLC), we investigated the serum MMP-9 of NSCLC patients and performed a meta-analysis of the published literature. The expression and activity of serum MMP-9 were assessed by ELISA and gelatin zymography in 163 NSCLC patients. Moreover, 26 studies were included in meta-analysis by searching Medline and ISI Web of Knowledge. Our own data revealed high activity but not expression of MMP-9 significantly correlated with advanced T category and positive metastasis. In contrast, the meta-analysis revealed that increased MMP-9 level indicate high T category (RR = 0.83, 95% CI: 0.73-0.94), tumor stage (RR = 0.72, 95% CI: 0.63-0.82) and poor OS (5-year overall survival, RR = 1.32, 95% CI: 1.19-1.48). Moreover, stratified analysis based on sample types found that high MMP-9 expression in tissue specimen but not serum was significant correlated with advanced T category (RR = 0.81, 95% CI: 0.72-0.92), tumor stage (RR = 0.69, 95% CI: 0.60-0.80) and poor 5-year OS (1.33, 95% CI: 1.18-1.50).In conclusion, the activity of MMP-9 was positively correlated with advanced T category and distant metastasis. Moreover, the meta-analysis revealed that overexpression of MMP-9 in tissue but not in serum was a risk factor of advanced T category, tumor stage and poor outcome.
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http://dx.doi.org/10.18632/oncotarget.7607DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951301PMC
April 2016

[Value of liver perfusion imaging of 256-slice CT 
in evaluation of the cirrhosis].

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2016 Jan;41(1):44-50

Department of Radiology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.

Objective: To investigate the value of liver perfusion imaging of 256-slice CT in evaluating the compensated and decompensated cirrhosis. 


Methods: A total of 20 patients with liver cirrhosis, who were confirmed by liver biopsy, clinical symptoms and imaging, were selected from December 2012 to June 2014. According to the results of liver biopsy and the Child-Pugh classification, the patients were divided into a compensated cirrhosis group (n=8) and a decompensated cirrhosis group (n=12). Eleven cases without liver and spleen diseases were served as a control group. All subjects were under the 256-CT liver perfusion (256-CTP). The data of CTP [hepatic arterial perfusion (HAP), portal venous perfusion (PVP), total liver perfusion (TLP), hepatic perfusion index (HPI)] were obtained according to liver perfusion type, and the data of CTP [liver perfusion (LP), peak enhanced (PE), time to peak (TTP), blood volume (BV)] were obtained according to general perfusion type. Spearman rank correlation was used to analyze the correlation of liver cirrhosis with perfusion parameters. The receiver operating characteristic (ROC) curve was used to predict liver cirrhosis, and the maximized Youden index was served as the optimal cutoff value, then the area under curve, sensitivity and specificity were calculated.


Results: The PVP, TLP and PE values in the control group, the compensated cirrhosis group and the decompensated cirrhosis group were (76.63±37.26), (38.78±16.13) and (36.14±
15.31) mL/(100 mL·min); (98.48±43.58), (55.63±14.47) and (54.41±20.81) mL/(100 mL·min); 
(55.62±18.25), (44.11±5.79) and (41.08±7.74) HU, respectively, showing a gradual downward trend and a significant difference among the 3 groups (all P <0.05). HPI values were (19.50±6.08)%, (31.81±16.48)% and (34.47±16.04)%; TTP values were (37.32±8.59), (47.06±14.61), (59.86±20.87) s, respectively, showing a gradual upward trend and significant difference among the 3 groups ( all P<0.05). There was no significant difference in the HAP, LP and BV among the 3 groups (all P>0.05). PVP, TLP, PE and LP were negatively correlated with the process of liver cirrhosis (r=-0.592, -0.567, -0.409, -0.569, all P<0.05), but HPI and TTP were positively correlated with the process of liver cirrhosis (r=0.434 and 0.538, both P<0.05). 


Conclusion: 256-CTP could provide useful information for the assessment of liver cirrhosis by measuring a plurality of perfusion parameters. The hepatic microvascular changes in patients with liver cirrhosis could be quantitatively assessed by perfusion CT. TTP shows high efficiency in prediction of liver cirrhosis and decompensated liver cirrhosis.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2016.01.007DOI Listing
January 2016

A pH- and Temperature-Responsive Magnetic Composite Adsorbent for Targeted Removal of Nonylphenol.

ACS Appl Mater Interfaces 2015 Nov 2;7(44):24446-57. Epub 2015 Nov 2.

College of Biology and the Environment, Nanjing Forestry University , Nanjing 210037, P.R. China.

A pH- and temperature-responsive magnetic adsorbent [poly(N-isopropylacrylamide) grafted chitosan/Fe3O4 composite particles, CN-MCP], was synthesized for the removal of the endocrine-disrupting chemical nonylphenol. According to the structural characteristics (changeable surface-charge and hydrophilic/hydrophobic properties) of the targeted contaminant, CN-MCP was designed owning special structure (pH- and temperature-responsiveness for the changeable surface-charge and adjustable hydrophilic/hydrophobic properties, respectively). Compared to chitosan magnetic composite particles without grafting modification (CS-MCP) and several other reported adsorbents, CN-MCP exhibited relatively high adsorption capacity for nonylphenol under corresponding optimal conditions (123 mg/g at pH 9 and 20 °C; 116 mg/g at pH 5 and 40 °C). Meanwhile, high selectivity of the novel adsorbent in selective adsorption of nonylphenol from bisolute solution of nonylphenol and phenol was found. Effects of grafting ratio of the grafted polymer branches and coexisting inorganic salts on the adsorption were systematically investigated. Moreover, CN-MCP demonstrated desired reusability during 20 times of adsorption-desorption recycling. The high adsorption capacity, high selectivity, and desired reusability aforementioned revealed the significant application potential of CN-MCP in the removal of NP. On the basis of the adsorption behaviors, isotherms equilibrium, thermodynamics and kinetics studies, and instrumental analyses including X-ray photoelectron spectroscopy, BET specific surface area, zeta potential, and static water contact angle measurements, distinct adsorption mechanisms were found under various conditions: charge attraction between CN-MCP and the contaminant, as well as binding between polymeric branches of CN-MCP and nonyls, contributed to the adsorption at pH 9 and 20 °C; whereas hydrophobic interaction between CN-MCP and nonylphenol played a dominant role at pH 5 and 40 °C. The current study provided a strategy for the structural design of adsorbents according to the features of targeted emerging contaminants, and the continuity of the work was discussed and proposed.
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http://dx.doi.org/10.1021/acsami.5b08709DOI Listing
November 2015
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