Publications by authors named "Jianping Zhang"

519 Publications

Genetic Polymorphism of rs211037 is Associated with Drug Response and Adverse Drug Reactions to Valproic Acid in Chinese Southern Children with Epilepsy.

Pharmgenomics Pers Med 2021 15;14:1141-1150. Epub 2021 Sep 15.

Department of Pharmacy, Baoan Women's and Children's Hospital, Jinan University, Shenzhen, 518102, People's Republic of China.

Background: Valproic acid (VPA) is recommended as a first-line treatment for children with epilepsy. polymorphism is found to be associated with epilepsy susceptibility and therapeutic response of anti-seizure medications (ASM); however, the role of in VPA treatment still remains unknown.

Objective: The purpose of this study was to explore the association of gene polymorphism with the drug response and adverse drug reactions (ADRs) related to VPA.

Methods: A retrospective study including 96 Chinese children with epilepsy treated by VPA was carried out. The ADRs were collected during VPA therapy and rs211037 in enrolled patients was genotyped using Sequenom MassArray system. A network pharmacological analysis involved protein-protein interaction and enrichment analysis was constructed to investigate the potential targets and pathways of on VPA-related ADRs.

Results: Among 96 patients, 41 individuals were defined as seizure together with 49 patients with seizure-free and 6 patients unclassified. Carriers of homozygote rs211037 CC genotype exhibited seizure-free to VPA ( = 0.042), whereas those with CT genotype showed seizure. Furthermore, CC genotype had predisposition to digestive ADRs ( = 0.037) but was a protective factor for VPA-associated weight gain ( = 0.013). Ten key genes related to digestive ADRs and weight gain induced by VPA were identified by network pharmacological analysis and mainly involved in "GABAergic synaptic signaling", "GABA receptor signaling", and "taste transduction" pathways/processes through enrichment analysis.

Conclusion: This study revealed that variation exerted a predictable role in the efficacy and safety of VPA treatment for Chinese children with epilepsy.
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http://dx.doi.org/10.2147/PGPM.S329594DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450188PMC
September 2021

Alkali-Resistant Catalytic Reduction of NO by Using Ce-O-B Alkali-Capture Sites.

Environ Sci Technol 2021 09 16;55(17):11970-11978. Epub 2021 Aug 16.

State Key Laboratory of Advanced Special Steel, School of Materials Science and Engineering, International Joint Laboratory of Catalytic Chemistry, College of Sciences, Shanghai University, No. 99 Shangda Road, Shanghai 200444, P. R. China.

Reducing the poisoning effect arising from alkali metals over catalysts for selective catalytic reduction (SCR) of NO by NH is still an urgent issue to be solved. Herein, alkali-resistant NO reduction over B-doped CeO/TiO catalysts (Ce-B/TiO) with Ce-O-B alkali-capture sites was originally demonstrated. It was noted that boron was confirmed to be doped into the lattice of CeO to form the Ce-O-B structure. In this way, more active Ce(III) species and oxygen vacancies were generated from B-doped CeO, thus accelerating the redox cycle and enhancing the adsorption/activation of NO. Gratifyingly, the created Ce-O-B sites as alkali-capture sites could be effectively combined with K and release the poisoned Ce active sites, which maintained efficient NH and NO adsorption/activation over K poisoned Ce-B/TiO. This work paves a way for designing highly efficient and alkali-resistant SCR catalysts in both academic and industrial fields.
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http://dx.doi.org/10.1021/acs.est.1c02882DOI Listing
September 2021

Long Noncoding RNA RP11-115N4.1 Promotes Inflammatory Responses by Interacting With HNRNPH3 and Enhancing the Transcription of HSP70 in Unexplained Recurrent Spontaneous Abortion.

Front Immunol 2021 13;12:717785. Epub 2021 Aug 13.

Department of Obstetrics and Gynecology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.

Background: Unexplained recurrent spontaneous abortion (URSA) is a common pregnancy complication and the etiology is unknown. URSA-associated lncRNAs are expected to be potential biomarkers for diagnosis, and might be related to the disease pathogenesis.

Objective: To investigate differential lncRNAs in peripheral blood of non-pregnant URSA patients and matched healthy control women and to explore the possible mechanism of differential lncRNAs leading to URSA.

Methods: We profiled lncRNAs expression in peripheral blood from 5 non-pregnant URSA patients and 5 matched healthy control women by lncRNA microarray analysis. Functions of URSA-associated lncRNAs were further investigated .

Results: RP11-115N4.1 was identified as the most differentially expressed lncRNA which was highly upregulated in peripheral blood of non-pregnant URSA patients ( = 3.63E-07, Fold change = 2.96), and this dysregulation was further validated in approximately 26.67% additional patients (4/15). RP11-115N4.1 expression was detected in both lymphocytes and monocytes of human peripheral blood, and overexpression of RP11-115N4.1 decreased cell proliferation in K562 cells significantly. Furthermore, heat-shock HSP70 genes ( and ) were found to be significantly upregulated upon RP11-115N4.1 overexpression by transcriptome analysis ( ( = 4.39E-08, Fold change = 4.17), ( = 2.26E-06, Fold change = 2.99)). RNA pull down and RNA immunoprecipitation assay (RIP) analysis demonstrated that RP11-115N4.1 bound to HNRNPH3 protein directly, which in turn activate heat-shock proteins (HSP70) analyzed by protein-protein interaction and knockdown assays. Most importantly, the high expression of HSP70 was also verified in the serum of URSA patients and the supernatant of K562 cells with RP11-115N4.1 activation, and HSP70 in supernatant can exacerbate inflammatory responses in monocytes by inducing IL-6, IL-1β, and TNF-α and inhibit the migration of trophoblast cells, which might associate with URSA.

Conclusion: Our results demonstrated that the activation of RP11-115N4.1 can significantly increase the protein level of HSP70 binding to HNRNPH3, which may modulate the immune responses and related to URSA. Moreover, RP11-115N4.1 may be a novel etiological biomarker and a new therapeutic target for URSA.
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http://dx.doi.org/10.3389/fimmu.2021.717785DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414257PMC
August 2021

Linum usitatissimum FAD2A and FAD3A enhance seed polyunsaturated fatty acid accumulation and seedling cold tolerance in Arabidopsis thaliana.

Plant Sci 2021 Oct 8;311:111014. Epub 2021 Aug 8.

State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi, 712100, China. Electronic address:

Flax (Linum usitatissimum) seed oil is rich in polyunsaturated fatty acids (PUFAs), particularly linolenic acid, which is converted from linoleic acid. Studies have indicated that the biosynthesis of linoleic acid and linolenic acid is controlled by FAD2 and FAD3, respectively. However, the functional distinctions of different LuFAD2 and LuFAD3 copies from L. usitatissimum in governing the biosynthesis of linoleic acid or linolenic acid, respectively, remain unclear. In this study, five LuFAD2 and three LuFAD3 cDNAs were cloned from the L. usitatissimum cultivar 'Longya 10', and GC-MS results demonstrated that LuFAD2A and LuFAD3A play predominant roles in the accumulation of linoleic acid and linolenic acid, respectively. Their simultaneous overexpression in Arabidopsis thaliana seeds led to a significant increase in fatty acid contents, especially PUFAs. Additionally, LuFAD2A and LuFAD3A promoted the biosynthesis of jasmonic acid by increasing the levels of linolenic acid, which, in turn, enhanced plant cold tolerance. When the amount of linolenic acid is not sufficient, plants adapt to low temperature via the accumulation of anthocyanins. These findings provide insights into the higher accumulation of PUFAs in L. usitatissimum seeds, and provide potential targets for improving oil quality of other oil-producing crops through molecular manipulation.
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http://dx.doi.org/10.1016/j.plantsci.2021.111014DOI Listing
October 2021

Engineering a Smart Agent for Enhanced Immunotherapy Effect by Simultaneously Blocking PD-L1 and CTLA-4.

Adv Sci (Weinh) 2021 Sep 2:e2102500. Epub 2021 Sep 2.

Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.

Combinations of immune checkpoint therapies show encouraging results in the treatment of many human cancers. However, the higher costs and greater side effects of such combinations compared with single-agent immunotherapies limit their further applications. In this work, a novel smart agent, [email protected] F-ZIF-8, is developed to overcome these limitations. KN046 is a novel recombinant humanized PD-L1/CTLA-4 bispecific single-domain antibody-Fc fusion protein, which can bind to both PD-L1 and CTLA-4 effectively. ZIF-8 is a smart delivery system, which can safely and effectively deliver KN406 to a tumor. In vitro and in vivo results demonstrate that the smart agent [email protected] F-ZIF-8 not only improves the immune response rate of the antibody drug in treatment of tumors but also reduces its toxic side effects, thereby achieving excellent antitumor efficacy. This study provides an engineering strategy for clinical applications of a more effective immunotherapy.
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http://dx.doi.org/10.1002/advs.202102500DOI Listing
September 2021

Capacitive Removal of Fluoride Ions via Creating Multiple Capture Sites in a Modulatory Heterostructure.

Environ Sci Technol 2021 09 24;55(17):11979-11986. Epub 2021 Aug 24.

International Joint Laboratory of Catalytic Chemistry, Department of Chemistry, Research Center of Nano Science and Technology, College of Sciences, State Key Laboratory of Advanced Special Steel, School of Materials Science and Engineering, Shanghai University, No.99 Shangda Road, Shanghai 200444, China.

Fluoride pollution has become a major concern because of its adverse effects on human health. However, the removal capacity of defluorination agents in traditional methods is far from satisfactory. Herein, capacitive removal of F ions via creating multiple capture sites in a modulatory heterostructure has been originally demonstrated. The heterostructure of uniformly dispersed AlO coating on hollow porous nitrogen-doped carbon frameworks was precisely synthesized by atomic layer deposition. An exceptional F ion removal efficiency at 1.2 V (95.8 and 92.9% in 5 and 10 mg/L F solutions, respectively) could be finally achieved, with a good regeneration ability after 20 consecutive defluorination cycles. Furthermore, we investigated the removal mechanisms of F ions by in situ Raman, in situ X-ray diffraction, and ex situ X-ray photoelectron spectroscopy measurements. The promotional removal capacity was realized by the multiple capture sites of the reversible conversion of Al-F species and the insertion of F ions into the carbon skeleton. This work offers an important new pathway and deep understanding for efficient removal of F ions from wastewater.
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http://dx.doi.org/10.1021/acs.est.1c03228DOI Listing
September 2021

Decoupling economic development from municipal solid waste generation in China's cities: Assessment and prediction based on Tapio method and EKC models.

Waste Manag 2021 Sep 4;133:37-48. Epub 2021 Aug 4.

School of Accounting, Guangdong University of Finance & Economics, Guangzhou, China; Institute of Capital Market and Audit Governance Studies for the Great Bay Area (Guangdong, Hong-Kong, Macau) of GDUFE, Guangzhou, China.

This study uses the Tapio elastic decoupling analysis method and an empirical model of the environmental Kuznets curve (EKC) to analyze the decoupling between municipal solid waste (MSW) generation and economic development in 285 of China's cities from 2002 to 2017. The decoupling analysis results show that the decoupling states in China's cities generally improved first and then deteriorated in 2002-2017. The proportion of cities experiencing deterioration of decoupling states had increased to 60.00% by 2014-2017, and cities with a higher economic development level generally had more serious deterioration. The empirical test results support the N-shaped EKC hypothesis of MSW, which can explain why the decoupling state changed from improvement to deterioration. The difference of the economic level of sample cities (which determines their position on EKC) can also explain the spatial heterogeneity of the decoupling state and its changes; for example, the proportion of cities that have crossed the inflection point of Tapio decoupling elasticity (the per capita GDP is 13,130.89 yuan) to enter the deterioration stage is highest (100/101) in the economically developed eastern region, and is lowest (66/75) in the economically backward western region of China. In general, the empirical results of EKC can effectively support and explain the results of decoupling analysis.
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http://dx.doi.org/10.1016/j.wasman.2021.07.034DOI Listing
September 2021

Construction of Novel lncRNA-miRNA-mRNA Network Associated With Recurrence and Identification of Immune-Related Potential Regulatory Axis in Hepatocellular Carcinoma.

Front Oncol 2021 15;11:626663. Epub 2021 Jul 15.

Department of General Surgery, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, China.

Hepatocellular carcinoma (HCC) is one of the most common malignant diseases globally. Despite continuous improvement of treatment methods, high postoperative recurrence rate remains an urgent problem. In order to determine the mechanism underlying recurrence of liver cancer and identify prognostic genes, data from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) were integrated and analyzed. Differentially expressed genes (DEGs) between HCC tissue and normal liver tissue were identified, and a protein-protein interaction network was constructed to find hub genes. Clinical correlation analysis and disease-free survival (DFS) analysis were performed using the R language and GEPIA to identify relapse-related genes. Correlation analysis was used to identify a potential regulatory axis. Dual-luciferase reporter gene assay was used to confirm the reliability of the long non-coding RNA (lncRNA)-microRNA (miRNA)-mRNA regulatory axis. Immune infiltration analysis was performed using the TIMER database. Correlations between immune gene markers and ASF1B were verified using quantitative real-time polymerase chain reaction (RT-qPCR). In this work, we found that nine lncRNAs and five mRNAs were significantly overexpressed in HCC tissues from patients with recurrence. , , , , , , and were also closely related to HCC grade and stage. Survival analysis showed that these seven DEGs were significantly correlated with poor DFS. Correlation analysis identified -- as a potential regulatory axis. Dual-luciferase reporter gene assay showed that SNHG3 and ASF1B directly bound to miR-214-3p. ASF1B was negatively regulated by miRNA-214-3p, and overexpression of SNHG3 could inhibit the expression of miRNA-214-3p. In addition, was positively correlated with immune infiltration. A reduction in ASF1B could markedly inhibit the expression of CD86, CD8, STAT1, STAT4, CD68, and PD1 in HCC cells. Flow cytometry showed that SNHG3 promoted the PD-1 expression by regulating ASF1B. Meanwhile, elevated predicted poor prognosis of HCC patients in subgroups with decreased B cells, CD8+ T cells, or neutrophils, and those with enriched CD4+ T cells. In conclusion, we found that a novel lncRNA SNHG3/miR-214-3p/ASF1B axis could promote the recurrence of HCC by regulating immune infiltration.
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http://dx.doi.org/10.3389/fonc.2021.626663DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8320021PMC
July 2021

Investigation of the ameliorative effects of baicalin against arsenic trioxide-induced cardiac toxicity in mice.

Int Immunopharmacol 2021 Oct 29;99:108024. Epub 2021 Jul 29.

Affiliated Hospital, Hebei University of Chinese Medicine, Shijiazhuang 050011, Hebei, China; School of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, China. Electronic address:

Baicalin (BA), a kind of flavonoids compound, comes from Scutellaria baicalensis Georgi (a kind of perennial herb) and has beneficial effects on the cardiovascular system through anti-oxidant, anti-inflammation, and anti-apoptosis actions. However, the therapeutic effects and latent mechanisms of BA on arsenic trioxide (ATO)-induced cardiac toxicity has not been reported. The present research was performed to explore the effects and mechanisms of BA on ATO-induced heart toxicity. Male Kunming mice were treated with ATO (7.5 mg/kg) to induce cardiac toxicity. After the mice received ATO, BA (50 and 100 mg/kg) was administered for estimating its cardioprotective effects. Statistical data demonstrated that BA treatment alleviated electrocardiogram abnormalities and pathological injury caused by ATO. BA could also lead to recovery of CK and LDH activities to normal range and cause a decrease in MDA levels and ROS generation, augmentation of SOD, CAT, and GSH activities. We also found that BA caused a reduction in the expression of proinflammatory cytokines, such as TNF-α and IL-6. Moreover, BA attenuated ATO-induced apoptosis by promoting the expression of Bcl-2 and suppressing the expression of Bax and caspase-3. TUNEL test result demonstrated BA caused impediment of ATO-induced apoptosis. Furthermore, BA treatment suppressed the high expression of TLR4, NF-κB and P-NF-κB caused by ATO. In conclusion, these results indicate that BA may alleviate ATO-induced cardiac toxicity by restraining oxidative stress, apoptosis, and inflammation, and its mechanism would be associated with the inhibition of the TLR4/NF-κB signaling pathway.
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http://dx.doi.org/10.1016/j.intimp.2021.108024DOI Listing
October 2021

Alkali-Resistant Catalytic Reduction of NO via Naturally Coupling Active and Poisoning Sites.

Environ Sci Technol 2021 Jul 29. Epub 2021 Jul 29.

State Key Laboratory of Advanced Special Steel, School of Materials Science and Engineering, International Joint Laboratory of Catalytic Chemistry, College of Sciences, Shanghai University, Shanghai 200444, China.

Releasing the poisoning effect of alkali metals over catalysts is still an intractable issue for selective catalytic reduction (SCR) of NO with ammonia. The presence of K in fly ash always dramatically suppressed catalytic activity by impairing acidity and redox properties, leading to severe reduction of lifetime for SCR catalysts. Herein, alkali-resistant NO reduction over TiO-supported Fe(SO) catalysts was originally demonstrated via naturally coupling active and poisoning sites. Notably, TiO-supported Fe(SO) catalysts expressed admirable NO conversion and K resistance within a quite broad temperature window of 200-500 °C. The catalysts with more conserved sulfate species revealed that sulfate groups preferred to migrate from the bulk phase to surface, thus effectively binding with K poisons to release the damage on iron active sites. Because of protection effects of migrated sulfates and closely coupling effects with Fe active sites, NH and NO adsorption amounts and rates were well maintained. In this way, Fe metal sites and sulfate species closely coupled together on a self-preserved TiO-supported Fe(SO) catalyst played essential roles as highly active sites and unique poisoning sites. This work paves a new way to design SCR catalysts with superior alkali resistance that are more reliable in practical deNO application.
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http://dx.doi.org/10.1021/acs.est.1c02061DOI Listing
July 2021

Fully Learnable Model for Task-Driven Image Compressed Sensing.

Sensors (Basel) 2021 Jul 7;21(14). Epub 2021 Jul 7.

College of Electronic Information and Optical Engineering, Nankai University, Tianjin 300350, China.

This study primarily investigates image sensing at low sampling rates with convolutional neural networks (CNN) for specific applications. To improve the image acquisition efficiency in energy-limited systems, this study, inspired by compressed sensing, proposes a fully learnable model for task-driven image-compressed sensing (FLCS). The FLCS, based on Deep Convolution Generative Adversarial Networks (DCGAN) and Variational Auto-encoder (VAE), divides the image-compressed sensing model into three learnable parts, i.e., the Sampler, the Solver and the Rebuilder. To be specific, a measurement matrix suitable for a type of image is obtained by training the Sampler. The Solver calculates the image's low-dimensional representation with the measurements. The Rebuilder learns a mapping from the low-dimensional latent space to the image space. All the mentioned could be trained jointly or individually for a range of application scenarios. The pre-trained FLCS reconstructs images with few iterations for task-driven compressed sensing. As indicated from the experimental results, compared with existing approaches, the proposed method could significantly improve the reconstructed images' quality while decreasing the running time. This study is of great significance for the application of image-compressed sensing at low sampling rates.
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http://dx.doi.org/10.3390/s21144662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309481PMC
July 2021

Genomics accelerated isolation of a new stem rust avirulence gene-wheat resistance gene pair.

Nat Plants 2021 Sep 22;7(9):1220-1228. Epub 2021 Jul 22.

Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food, Canberra, Australian Capital Territory, Australia.

Stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt) is a devastating disease of the global staple crop wheat. Although this disease was largely controlled in the latter half of the twentieth century, new virulent strains of Pgt, such as Ug99, have recently evolved. These strains have caused notable losses worldwide and their continued spread threatens global wheat production. Breeding for disease resistance provides the most cost-effective control of wheat rust diseases. A number of rust resistance genes have been characterized in wheat and most encode immune receptors of the nucleotide-binding leucine-rich repeat (NLR) class, which recognize pathogen effector proteins known as avirulence (Avr) proteins. However, only two Avr genes have been identified in Pgt so far, AvrSr35 and AvrSr50 (refs. ), and none in other cereal rusts. The Sr27 resistance gene was first identified in a wheat line carrying an introgression of the 3R chromosome from Imperial rye. Although not deployed widely in wheat, Sr27 is widespread in the artificial crop species Triticosecale (triticale), which is a wheat-rye hybrid and is a host for Pgt. Sr27 is effective against Ug99 (ref. ) and other recent Pgt strains. Here, we identify both the Sr27 gene in wheat and the corresponding AvrSr27 gene in Pgt and show that virulence to Sr27 can arise experimentally and in the field through deletion mutations, copy number variation and expression level polymorphisms at the AvrSr27 locus.
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http://dx.doi.org/10.1038/s41477-021-00971-5DOI Listing
September 2021

Coking-resistant dry reforming of methane over Ni/γ-AlO catalysts by rationally steering metal-support interaction.

iScience 2021 Jul 17;24(7):102747. Epub 2021 Jun 17.

International Joint Laboratory of Catalytic Chemistry, Department of Chemistry, Research Center of Nano Science and Technology, College of Sciences, Shanghai University, 200444 Shanghai, China.

The coking issue is the main challenge for dry reforming of methane (DRM) over Ni-based catalysts. Herein, we excavate the reasons for the enhanced coking resistance of the bounded Ni over the free state Ni in Ni/γ-AlO catalysts for DRM. Rational metal-support interaction of the bounded Ni would facilitate desorption of CO, thus suppressing CO disproportionation and decreasing carbon deposition. The higher activity of the bounded Ni is ascribed to better methane cracking ability, stronger adsorption, and activation of CO by forming polydentate carbonate. The better activation of CO over the bounded Ni would also contribute to the gasification of formed coke. We gain an insight into the anti-coking mechanism of DRM determined by metal-support interaction in Ni/γ-AlO catalysts through mechanistic studies. It is believed that our findings would enlighten the design of more efficient catalysts for DRM.
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http://dx.doi.org/10.1016/j.isci.2021.102747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261659PMC
July 2021

Molecular identification and genetic-polymorphism analysis of Fasciola flukes in Dali Prefecture, Yunnan Province, China.

Parasitol Int 2021 Dec 2;85:102416. Epub 2021 Jul 2.

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research); NHC Key Laboratory of Parasite and Vector Biology; WHO Collaborating Centre for Tropical Diseases, Shanghai 200025, China. Electronic address:

This study aimed to identify species of Fasciola flukes in Dali Prefecture (Yunnan Province, China) and analyze their genetic diversity. Fasciola flukes (n = 122) were collected from cattle livers in a farmers' market in Xiaguan Town, Dali Prefecture. Nucleotide sequences of ribosomal internal transcribed spacer (ITS) as well as nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) and mitochondrial cytochrome c oxidase subunit 1 (CO1) were amplified, sequenced, and subjected to homology analysis. The heterozygosity ratios of different ITS alleles were determined using the peak-height ratio of heterozygous loci. Multiplex PCR analysis of the nuclear protein coding gene, phosphoenolpyruvate carboxykinase (pepck), was used to identify Fasciola species. Multiple ND1 sequence alignments enabled further genetic diversity analysis of regional Fasciola flukes. Seven ITS sequences belonged to F. hepatica and 115 belonged to Fh/Fg heterozygous flukes. Sequencing analysis of heterozygous flukes revealed 11 heterozygous loci with double peaks, with significantly variable ratios among individuals. ND1 and CO1 results indicated that one specimen was identical to F. hepatica, while 121 specimens were identical to F. gigantica or contained one variable site. Multiplex PCR results for pepck showed that double bands for F. hepatica and F. gigantica were amplified from Dali Fasciola specimens; hence, they were all heterozygous. By combining ITS, ND1, and CO1 sequences with multiplex pepck PCR results, all 122 specimens were identified as Fh/Fg heterozygous Fasciola flukes. Our experimental results preliminarily confirmed a high degree of Fh/Fg heterozygosity among Fasciola flukes in the Dali area. Selecting multiple molecular markers for concurrent analysis will provide more comprehensive and accurate genetic information.
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http://dx.doi.org/10.1016/j.parint.2021.102416DOI Listing
December 2021

Resistance Mechanism to Metsulfuron-Methyl in .

Plants (Basel) 2021 Jun 28;10(7). Epub 2021 Jun 28.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400, China.

is a common winter weed in China and other Asia countries. We have previously found a biotype (R) resistant to acetyl co-enzyme A carboxylase (ACCase) herbicides also cannot be effectively controlled by some acetolactate synthase (ALS) herbicides. This study evaluated the level of resistance to four ALS herbicides (metsulfuron-methyl, chlorsulfuron, monosulfuron, pyribambenz isopropyl) in the R biotype and the associated resistance mechanism. The R biotype exhibited moderate level of resistance to metsulfuron-methyl (6.0-fold) compared with the sensitive biotype (S). Sequence analysis of gene revealed that two genes existed in . However, no substitution associated with ALS resistance mechanism were found in genes between the S and R biotypes. The activity of ALS enzyme isolated from the R biotype was inherently higher and less sensitive to metsulfuron-methyl than the S biotype. Glutathione S-transferases (GST) activity was also less sensitive to metsulfuron-methyl in the R than as the S biotypes. Malathion, a cytochrome P450 (CYP) monooxygenase inhibitor, had much greater synergistic effect with metsulfuron-methyl on the R than as the S plants, reducing the ED value (herbicide dose to inhibit growth by 50%) of metsulfuron-methyl by 23- and 6-fold, respectively, suggesting that CYP mediated enhanced metabolism might contribute to the resistance to ALS herbicides. These results suggest that metsulfuron-methyl resistance in the R biotype was associated with the up-regulated ALS enzymatic activity and the GST and CYP-mediated enhanced herbicide metabolism.
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http://dx.doi.org/10.3390/plants10071309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308939PMC
June 2021

CMMCSegNet: Cross-Modality Multicascade Indirect LGE Segmentation on Multimodal Cardiac MR.

Comput Math Methods Med 2021 5;2021:9942149. Epub 2021 Jun 5.

School of Mathematics and Computational Science, Xiangtan University, Xiangtan, Hunan 411105, China.

Since Late-Gadolinium Enhancement (LGE) of cardiac magnetic resonance (CMR) visualizes myocardial infarction, and the balanced-Steady State Free Precession (bSSFP) cine sequence can capture cardiac motions and present clear boundaries; multimodal CMR segmentation has played an important role in the assessment of myocardial viability and clinical diagnosis, while automatic and accurate CMR segmentation still remains challenging due to a very small amount of labeled LGE data and the relatively low contrasts of LGE. The main purpose of our work is to learn the real/fake bSSFP modality with ground truths to indirectly segment the LGE modality of cardiac MR by using a proposed cross-modality multicascade framework: cross-modality translation network and automatic segmentation network, respectively. In the segmentation stage, a novel multicascade pix2pix network is designed to segment the fake bSSFP sequence obtained from a cross-modality translation network. Moreover, we propose perceptual loss measuring features between ground truth and prediction, which are extracted from the pretrained vgg network in the segmentation stage. We evaluate the performance of the proposed method on the multimodal CMR dataset and verify its superiority over other state-of-the-art approaches under different network structures and different types of adversarial losses in terms of dice accuracy in testing. Therefore, the proposed network is promising for Indirect Cardiac LGE Segmentation in clinical applications.
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http://dx.doi.org/10.1155/2021/9942149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203380PMC
June 2021

Improved NO Reduction over Phosphate-Modified FeO/TiO Catalysts Tailoring Reaction Paths by Creating Alkali-Poisoning Sites.

Environ Sci Technol 2021 07 18;55(13):9276-9284. Epub 2021 Jun 18.

International Joint Laboratory of Catalytic Chemistry, State Key Laboratory of Advanced Special Steel, Department of Chemistry, Research Center of Nano Science and Technology, College of Sciences, Shanghai University, Shanghai 200444, China.

The deactivation issue arising from alkali poisoning over catalysts is still a challenge for the selective catalytic reduction of NO by NH. Herein, improved NO reduction in the presence of alkaline metals over phosphate-modified FeO/TiO catalysts has been originally demonstrated tailoring the reaction paths by creating alkali-poisoning sites. The introduction of phosphate results in the partial formation of iron phosphate species and makes the catalyst to mainly exhibit the characteristics of FePO, which is responsible for the widened temperature window and enhanced alkali resistance. The tetrahedral [FeO]/[PO] structures in iron phosphate act as the Brønsted acid sites to increase the catalyst surface acidity. In addition, the formation of an Fe-O-P structure enhances the redox ability and increases surface adsorbed oxygen. Furthermore, the created phosphate groups (PO) serving as alkali-poisoning sites preferentially combine with potassium so that iron species on the active sites are protected. Therefore, the enhanced NH species adsorption capacity, improved redox ability, and active nitrate species remaining in the phosphate-modified FeO/TiO catalyst ensure the de-NO activity after being poisoned by alkali metals through the Langmuir-Hinshelwood reaction pathway. Hopefully, this novel strategy could provide an inspiration to design novel catalysts to control NO emission with extraordinary resistance to alkaline metals.
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http://dx.doi.org/10.1021/acs.est.1c01722DOI Listing
July 2021

Downregulation of ACE2 expression by SARS-CoV-2 worsens the prognosis of KIRC and KIRP patients via metabolism and immunoregulation.

Int J Biol Sci 2021 10;17(8):1925-1939. Epub 2021 May 10.

School of Pharmacy, Jinan University, Guangzhou 510630, China.

Angiotensin-converting enzyme 2 (ACE2) and transmembrane serine protease 2 (TMPRSS2) allow entry of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) into host cells and play essential roles in cancer therapy. However, the functions of ACE2 and TMPRSS2 in kidney cancer remain unclear, especially as kidneys are targets for SARS-CoV-2 infection. UCSC Xena project, the Cancer Genome Atlas (TCGA), and Gene Expression Omnibus (GEO) databases (GSE30589 and GSE59185) were searched for gene expression in human tissues, gene expression data, and clinical information. Several bioinformatics methods were utilized to analyze the correlation between ACE2 and TMPRSS2 with respect to the prognosis of kidney renal clear cell carcinoma (KIRC) and kidney renal papillary cell carcinoma (KIRP). ACE2 expression was significantly upregulated in tumor tissue, while its downregulation was associated with low survival in KIRC and KIRP patients. TMPRSS2 was downregulated in KIRC and KIRP, and its expression was not correlated with patient survival. According to clinical risk factor-based prediction models, ACE2 exhibits predictive accuracy for kidney cancer prognosis and is correlated with metabolism and immune infiltration. In an animal model, ACE2 expression was remarkably downregulated in SARS-CoV-2-infected cells compared to in the control. ACE2 expression is highly correlated with various metabolic pathways and is involved in immune infiltration.it plays a crucial role than TMPRSS2 in diagnosing and prognosis of kidney cancer patients. The overlap in ACE2 expression between kidney cancer and SARS-CoV-2 infection suggests that patients with KIRC or KIRP are at high risk of developing serious symptoms.
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http://dx.doi.org/10.7150/ijbs.57802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193256PMC
June 2021

Mortalin/glucose-regulated protein 75 promotes the cisplatin-resistance of gastric cancer via regulating anti-oxidation/apoptosis and metabolic reprogramming.

Cell Death Discov 2021 Jun 11;7(1):140. Epub 2021 Jun 11.

Department of General Surgery, The Affiliated Changzhou No. 2 Hospital of Nanjing Medical University, Changzhou, China.

Platinum drug treatment is one of the most predominant chemotherapeutic strategies for patients with gastric cancer (GC). However, the therapeutic effect is less than satisfactory, largely due to the acquired resistance to platinum drugs. Therefore, a better understanding of the underlying mechanisms can greatly improve the therapeutic efficacy of GC. In this study, we aimed to investigate the chemo-resistance related functions/mechanisms and clinical significance of glucose-regulated protein 75 (GRP75) in GC. Here, our data showed that compared with SGC7901 cells, the expression of GRP75 was markedly higher in cisplatin-resistance cells (SGC7901). Knockdown of GRP75 abolished the maintenance of mitochondrial membrane potential (MMP) and inhibited the nuclear factor erythroid-2-related factor 2 (NRF2), phosphatidylinositol 3 kinase/protein kinase B (PI3K/AKT), hypoxia-inducible factor 1α (HIF-1α), and c-myc, which resulted in blocking the activation of their downstream targets. These processes attenuated the anti-oxidation/apoptosis abilities and altered the metabolic reprogramming in SGC7901 cells, leading to re-sensitizing these cells to cisplatin. However, overexpression of GRP75 in SGC7901 cells caused the opposite effects. A xenografts model confirmed the abovementioned results. In GC patients receiving platinum chemotherapy and a meta-analysis, a high level of GRP75 was positively associated with aggressive characteristics and poor prognosis including but not limited to gastrointestinal cancers, and was an independent predictor for overall survival. Collectively, our study indicated that GRP75 was involved in the cisplatin-resistance of GC and that GRP75 could be a potential therapeutic target for restoring the drug response in platinum-resistance cells and a useful additive prognostic tool in guiding clinical management of GC patients.
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http://dx.doi.org/10.1038/s41420-021-00517-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196146PMC
June 2021

A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes.

Nat Commun 2021 06 7;12(1):3378. Epub 2021 Jun 7.

Plant Breeding Institute, School of Life and Environmental Sciences, University of Sydney, Cobbitty, NSW, Australia.

The re-emergence of stem rust on wheat in Europe and Africa is reinforcing the ongoing need for durable resistance gene deployment. Here, we isolate from wheat, Sr26 and Sr61, with both genes independently introduced as alien chromosome introgressions from tall wheat grass (Thinopyrum ponticum). Mutational genomics and targeted exome capture identify Sr26 and Sr61 as separate single genes that encode unrelated (34.8%) nucleotide binding site leucine rich repeat proteins. Sr26 and Sr61 are each validated by transgenic complementation using endogenous and/or heterologous promoter sequences. Sr61 orthologs are absent from current Thinopyrum elongatum and wheat pan genome sequences, contrasting with Sr26 where homologues are present. Using gene-specific markers, we validate the presence of both genes on a single recombinant alien segment developed in wheat. The co-location of these genes on a small non-recombinogenic segment simplifies their deployment as a gene stack and potentially enhances their resistance durability.
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http://dx.doi.org/10.1038/s41467-021-23738-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184838PMC
June 2021

DMC-Fusion: Deep Multi-Cascade Fusion With Classifier-Based Feature Synthesis for Medical Multi-Modal Images.

IEEE J Biomed Health Inform 2021 Sep 3;25(9):3438-3449. Epub 2021 Sep 3.

Multi-modal medical image fusion is a challenging yet important task for precision diagnosis and surgical planning in clinical practice. Although single feature fusion strategy such as Densefuse has achieved inspiring performance, it tends to be not fully preserved for the source image features. In this paper, a deep multi-fusion framework with classifier-based feature synthesis is proposed to automatically fuse multi-modal medical images. It consists of a pre-trained autoencoder based on dense connections, a feature classifier and a multi-cascade fusion decoder with separately fusing high-frequency and low-frequency. The encoder and decoder are transferred from MS-COCO datasets and pre-trained simultaneously on multi-modal medical image public datasets to extract features. The feature classification is conducted through Gaussian high-pass filtering and the peak signal to noise ratio thresholding, then feature maps in each layer of the pre-trained Dense-Block and decoder are divided into high-frequency and low-frequency sequences. Specifically, in proposed feature fusion block, parameter-adaptive pulse coupled neural network and l-weighted are employed to fuse high-frequency and low-frequency, respectively. Finally, we design a novel multi-cascade fusion decoder on total decoding feature stage to selectively fuse useful information from different modalities. We also validate our approach for the brain disease classification using the fused images, and a statistical significance test is performed to illustrate that the improvement in classification performance is due to the fusion. Experimental results demonstrate that the proposed method achieves the state-of-the-art performance in both qualitative and quantitative evaluations.
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http://dx.doi.org/10.1109/JBHI.2021.3083752DOI Listing
September 2021

The Effects of LIT and MLR-Bf on Immune Biomarkers and Pregnancy Outcomes in Women With Previous Early Recurrent Miscarriage: A Retrospective Study.

Front Immunol 2021 4;12:642120. Epub 2021 May 4.

Department of Obstetrics and Gynecology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, China.

Immunological failure during pregnancy is considered one of the etiologies of recurrent miscarriage (RM). The decreased production of mixed lymphocyte reaction-blocking factors (MLR-Bf) may play a major role in this condition. Lymphocyte immunotherapy (LIT), which induces the production of MLR-Bf, has been used in treating RM patients since 1984. However, the effectiveness of LIT is currently being heatedly debated. In addition to that, possible changes to the maternal immune system upon induced MLR-Bf production by LIT remains unclear. To explore the possible impacts that MLR-Bf may have on the expression of immune biomarkers and pregnancy outcomes, and deduce whether the prevention of miscarriages is possible with LIT or MLR-Bf in RM patients. Women with previous early RM (eRM) were enrolled in this retrospective study after they got pregnant again. LIT was implemented before pregnancy and during the first trimester. MLR-Bf and immune biomarkers were checked as the clinical routine. Patients were followed up until 12 gestational weeks. Levels of immune biomarkers and successful pregnancy rates were compared between MLR-Bf group and MLR-Bf group stratified by LIT. Independent associations between LIT, or MLR-Bf, and miscarriage were estimated. All data management and analysis were conducted using SPSS 20.0. A total of 1,038 patients, 497 MLR-Bf (49 cases accepted LIT), and 541 MLR-Bf(463 cases induced by LIT) were included in the study. Percentage of lymphocytes, the ratio of CD4+ T cells/lymphocytes, and levels of some rheumatoid biomarkers (anti-U1-nRNP, anti-SAA-52kd, and anti-CENOP B) were statistically higher in MLR-Bf group than in MLR-Bf group among women without LIT. With LIT treatment the successful pregnancy rate was statistically higher in MLR-Bf group than in MLR-Bf group (66.7% vs. 51.0%, = 0.028) among women with LIT. Meanwhile, LIT was estimated to have an independent negative association with miscarriage. Upon LIT treament levels of immune biomarkers were different in women with and without MLR-Bf when stratified by whether they received LIT. Not MLR-Bf, but LIT, has an independent protective effect on miscarriage.
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http://dx.doi.org/10.3389/fimmu.2021.642120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129162PMC
September 2021

Selective Capacitive Removal of Heavy Metal Ions from Wastewater over Lewis Base Sites of S-Doped Fe-N-C Cathodes an Electro-Adsorption Process.

Environ Sci Technol 2021 06 13;55(11):7665-7673. Epub 2021 May 13.

International Joint Laboratory of Catalytic Chemistry, College of Sciences, State Key Laboratory of Advanced Special Steel, School of Materials Science and Engineering, Shanghai University, No. 99 Shangda Road, Shanghai 200444, China.

The pollution of toxic heavy metals is becoming an increasingly important issue in environmental remediation because these metals are harmful to the ecological environment and human health. Highly efficient selective removal of heavy metal ions is a huge challenge for wastewater purification. Here, highly efficient selective capacitive removal (SCR) of heavy metal ions from complex wastewater over Lewis base sites of S-doped Fe-N-C cathodes was originally performed an electro-adsorption process. The SCR efficiency of heavy metal ions can reach 99% in a binary mixed solution [NaCl (100 ppm) and metal nitrate (10 ppm)]. Even the SCR efficiency of heavy metal ions in a mixed solution containing NaCl (100 ppm) and multicomponent metal nitrates (10 ppm for each) can approach 99%. Meanwhile, the electrode also demonstrated excellent cycle performance. It has been demonstrated that the doping of S can not only enhance the activity of Fe-N sites and improve the removal ability of heavy metal ions but also combine with heavy metal ions by forming covalent bonds of S clusters on Lewis bases. This work demonstrates a prospective way for the selective removal of heavy metal ions in wastewater.
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http://dx.doi.org/10.1021/acs.est.1c01483DOI Listing
June 2021

Homogeneity and Stability Evaluation of External Quality Assessment Control Materials for Four Coagulation Tests.

Clin Lab 2021 May;67(5)

Background: This study assessed the homogeneity and stability of control materials used in external quality assessment (EQA) of four coagulation tests, aiming to verify that these materials meet clinical testing requirements and to provide an evidence base for future improvement of laboratory coagulation test quality.

Methods: The homogeneity and stability of control materials were assessed according to the relevant guidance. Homogeneity assessment involved 10 vials of samples obtained from 2 batches (each vial tested twice). The homogeneity of control materials in four coagulation tests was assessed using one-way analysis of variance, with standard deviation of uniformity (Ss) 0.3 σ as an assessment criterion. Stability assessment involved two vials of sam-ples obtained from two batches (each vial tested twice). The stability of control materials was assessed at cold storage, room temperature, temperature of 37°C. Reconstitution stability of control materials placed in cold storage and at room temperature, and long-term stability of reconstituted control materials stored frozen (-20°C and -80°C) were observed. Linear regression analysis was performed to assess long-term stability.

Results: The Ss values of EQA control materials for four coagulation tests were PT L1 Ss = 0.084, PT L2 Ss = 0.889, APTT L1 Ss = 0.164, APTT L2 Ss = 0.223, Fbg L1 Ss = 6.256, Fbg L2 Ss = 2.251, TT L1 Ss = 0.552, TT L2 Ss = 0.3111. PT, APTT, Fbg, and TT were associated with the standard deviation of uniformity values of 0.3 σ. Non-reconstituted samples were observed at 37°C for 2 hours and 4 hours, and at room temperature for 1 day. Reconstituted samples were observed when stored at 4°C for 4 hours and 8 hours, at room temperature for 4 hours, and at -20°C and -80°C for 6 months. Instability of reconstituted samples was observed in PT and APTT tests at 4°C for 8 hours and at -20°C for 5 months.

Conclusions: EQA control materials presented with satisfactory homogeneity in four coagulation tests. Non-reconstituted samples presented with satisfactory stability at 37°C for 2 hours and 4 hours and at room temperature for 1 day, while reconstituted samples presented with satisfactory stability when refrigerated at 4°C for 4 hours, when kept at room temperature for 4 hours, and when frozen at -80°C for 6 months.
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http://dx.doi.org/10.7754/Clin.Lab.2020.200911DOI Listing
May 2021

Intervention study of Snyder's hope theory on the stigma of stroke in young and middle-aged patients: a randomised trial.

Ann Palliat Med 2021 May 7;10(5):5721-5728. Epub 2021 May 7.

Department of Rehabilitation Medicine, Affiliated Haian Hospital of Nantong University, Nantong, China.

Background: Stigma refers to the sense of discrimination that society has towards a certain group of people, and this part of the group will also have a sense of shame due to their own negative circumstance. Young and middle-aged stroke patients need long-term effective support from their families and society, which can easily produce shame, and have a negative impact on disease treatment.

Methods: A total of 94 young and middle-aged stroke patients admitted to our hospital from November 2018 to November 2020 were selected and randomly allocated to 2 groups, with 47 cases in each group. The control group received routine intervention, and the observation group received SHT intervention. The stigma, hope level, compliance with functional exercise, National Institute of Health Stroke Scale (NIHSS) score and changes in activities of daily living were compared between the 2 groups before and after intervention.

Results: The total scores of social interaction, treatment, ability, and stigma of the observation group were lower than those of the control group after 1 month of intervention (P<0.05). The observation group's positive attitude towards reality and the future, taking positive actions, maintaining close relationships with others, and hope levels after 1 month of intervention were all higher than the control group (P<0.05). The functional exercise compliance of the observation group was higher than that of the control group after 1 month of intervention (P<0.05). The Barthel index score of the observation group was higher than that of the control group after 1 month of intervention, and the NIHSS score was lower than that of the control group (P<0.05).

Conclusions: The application of SHT to young and middle-aged stroke patients can reduce stigma, improve hope level and compliance with functional exercise, improve neurological function, and enhance the ability for daily living.

Trial Registration: Chinese Clinical Trial Registry ChiCTR2100045926.
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http://dx.doi.org/10.21037/apm-21-441DOI Listing
May 2021

The prediction of survival in Gastric Cancer based on a Robust 13-Gene Signature.

J Cancer 2021 12;12(11):3344-3353. Epub 2021 Apr 12.

Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Gastric cancer represents a major public health problem. Owing to the great heterogeneity of GC, conventional clinical characteristics are limited in the accurate prediction of individual outcomes and survival. This study aimed to establish a robust gene signature to predict the prognosis of GC based on multiple datasets. Initially, we downloaded raw data from four independent datasets of The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO), and performed univariate Cox proportional hazards regression analysis to identify prognostic genes associated with overall survival (OS) from each dataset. Thirteen common genes from four datasets were screened as candidate prognostic signatures. Then, a risk score model was developed based on this 13‑gene signature and validated by four independent datasets and the entire cohort. Patients with a high-risk score had poorer OS and recurrence-free survival (RFS). Multivariate regression and stratified analysis revealed that the 13-gene signature was not only an independent predictive factor but also associated with recurrence when adjusting for other clinical factors. Furthermore, in the high-risk group, gene set enrichment analysis (GSEA) showed that the mTOR signaling pathway and MAPK signaling pathway were significantly enriched. The present study provided a robust and reliable gene signature for prognostic prediction of both OS and RFS of patients with GC, which may be useful for delivering individualized management of patients.
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http://dx.doi.org/10.7150/jca.49658DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100809PMC
April 2021

High-temperature wheat leaf rust resistance gene Lr13 exhibits pleiotropic effects on hybrid necrosis.

Mol Plant 2021 Jul 6;14(7):1029-1032. Epub 2021 May 6.

College of Plant Protection, State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, Hebei 071001, China. Electronic address:

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http://dx.doi.org/10.1016/j.molp.2021.05.009DOI Listing
July 2021

Wheat leaf rust resistance gene Lr13 is a specific Ne2 allele for hybrid necrosis.

Mol Plant 2021 Jul 6;14(7):1025-1028. Epub 2021 May 6.

University of Sydney, Plant Breeding Institute, School of Life and Environmental Sciences, 107 Cobbitty Road, Cobbitty, NSW 2570, Australia. Electronic address:

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http://dx.doi.org/10.1016/j.molp.2021.05.010DOI Listing
July 2021

Long noncoding RNA LINC00641 promotes renal cell carcinoma progression via sponging microRNA-340-5p.

Cancer Cell Int 2021 Apr 14;21(1):210. Epub 2021 Apr 14.

Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.

Background: Emerging evidences have revealed that long non-coding RNAs (lncRNAs) have played critical roles in tumor occurrence and progression. LINC00641 has been reported to be involved in the initiation and development of several cancers in the recent years. However, the detailed biological role of LINC00641 in renal cell carcinoma (RCC) remains largely unclear.

Methods: In this study, the expression and biological function of LINC00641 were assessed in renal carcinoma both in vitro and in vivo. Cell proliferation, migration and colony formation assay were performed to explore the effect of LINC00641on growth, progression and invasion of RCC cell. qRT-PCR, flow cytometry and luciferase reporter assay and in vivo tumorigenicity assay were also carried out.

Results: The expression of LINC00641 was overexpressed in RCC tissues and cell lines, and high LINC00641 expression was correlated with tumor-node-metastasis stage. Furthermore, Silencing of LINC00641 remarkably inhibited the ability of cell proliferation, colony formation, and invasive capacities, as well as increasing the apoptotic rates of RCC cells in vitro. Mechanistically, miR-340-5p was validated to be targeted by LINC00641 and knockdown of miR-340-5p counteracted LINC00641 silencing-mediated inhibition of RCC progression. In addition, in vivo experiment confirmed the findings discovered in vitro.

Conclusions: These results suggested that LINC00641 promoted the progression of RCC by sponging miR-340-5p.
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http://dx.doi.org/10.1186/s12935-021-01895-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048250PMC
April 2021

Impacts of Pharmacists-Managed Oncology Outpatient Clinic on Resolving Drug-Related Problems in Ambulatory Neoplasm Patients: A Prospective Study in China.

Inquiry 2021 Jan-Dec;58:469580211009662

Department of Pharmacy, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Pharmacists are health care professionals who are actively involved in identifying and solving drug-related problems (DRPs) in neoplasm patients. However, the effectiveness of pharmaceutical services at outpatient clinic for neoplasm patients have not been reported in China. This study aims to describe and investigate the impacts of pharmacists-managed oncology outpatient clinic on ambulatory neoplasm patients. We performed a descriptive, prospective study from June 6, 2018 to June 6, 2020. Firstly, we established a pharmacists-managed oncology outpatient clinic and a Pharmacists Work System of Medication Therapy Management (MTM) software with the cooperation of oncologists, pharmacists and software engineers in 2018. Subjects were neoplasm patients who visited the pharmacists-managed outpatient clinic. The pharmacists performed a comprehensive assessment of the patient's medication and made planned interventions based on the DRPs identified. A total of 215 eligible patients with 707 visits were enrolled and recorded in the MTM software. A total of 316 DRPs (1.47 per patient) were identified. Adverse reactions, non-adherence, untreated indication, and drug interactions were the leading DRPs. 261 (82.6%) of the identified DRPs had been confirmed as resolved and 104 (78.2%) of adverse reactions were improved following pharmacist interventions and 2 to 3 course follow-up. Of the 382 planned interventions, 345 (90.3%) were accepted by patients or physicians. This is the first pharmacists-managed oncology outpatient clinic to describe the type of DRPs in neoplasm patients and evaluate the effectiveness of pharmacist interventions in China. Pharmacist interventions were efficacious in resolving DRPs and improving adverse reactions. We confirmed that pharmacists have an important role in ambulatory neoplasm patients care.
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http://dx.doi.org/10.1177/00469580211009662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047945PMC
April 2021
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