Publications by authors named "Jianping Ou"

14 Publications

  • Page 1 of 1

Homology-based repair induced by CRISPR-Cas nucleases in mammalian embryo genome editing.

Protein Cell 2021 May 4. Epub 2021 May 4.

MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.

Recent advances in genome editing, especially CRISPR-Cas nucleases, have revolutionized both laboratory research and clinical therapeutics. CRISPR-Cas nucleases, together with the DNA damage repair pathway in cells, enable both genetic diversification by classical non-homologous end joining (c-NHEJ) and precise genome modification by homology-based repair (HBR). Genome editing in zygotes is a convenient way to edit the germline, paving the way for animal disease model generation, as well as human embryo genome editing therapy for some life-threatening and incurable diseases. HBR efficiency is highly dependent on the DNA donor that is utilized as a repair template. Here, we review recent progress in improving CRISPR-Cas nuclease-induced HBR in mammalian embryos by designing a suitable DNA donor. Moreover, we want to provide a guide for producing animal disease models and correcting genetic mutations through CRISPR-Cas nuclease-induced HBR in mammalian embryos. Finally, we discuss recent developments in precise genome-modification technology based on the CRISPR-Cas system.
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http://dx.doi.org/10.1007/s13238-021-00838-7DOI Listing
May 2021

A Radar Signal Recognition Approach via IIF-Net Deep Learning Models.

Comput Intell Neurosci 2020 28;2020:8858588. Epub 2020 Aug 28.

School of Computer and Communication Engineering, Changsha University of Science and Technology, Changsha 410114, China.

In the increasingly complex electromagnetic environment of modern battlefields, how to quickly and accurately identify radar signals is a hotspot in the field of electronic countermeasures. In this paper, USRP N210, USRP-LW N210, and other general software radio peripherals are used to simulate the transmitting and receiving process of radar signals, and a total of 8 radar signals, namely, Barker, Frank, chaotic, P1, P2, P3, P4, and OFDM, are produced. The signal obtains time-frequency images (TFIs) through the Choi-Williams distribution function (CWD). According to the characteristics of the radar signal TFI, a global feature balance extraction module (GFBE) is designed. Then, a new IIF-Net convolutional neural network with fewer network parameters and less computation cost has been proposed. The signal-to-noise ratio (SNR) range is -10 to 6 dB in the experiments. The experiments show that when the SNR is higher than -2 dB, the signal recognition rate of IIF-Net is as high as 99.74%, and the signal recognition accuracy is still 92.36% when the SNR is -10 dB. Compared with other methods, IIF-Net has higher recognition rate and better robustness under low SNR.
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http://dx.doi.org/10.1155/2020/8858588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474755PMC
August 2020

Diagnostic performance of discontinuous density gradient centrifugation for estimating human semen quality.

Andrology 2021 01 9;9(1):196-203. Epub 2020 Sep 9.

Department of Infertility and Sexual Medicine, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.

Background: Semen analysis plays an important role in the diagnosis of male infertility. However, many studies have demonstrated that the current methods of semen analysis are inefficient for assessing male fertility.

Objective: To test whether prior discontinuous density gradient centrifugation (DDCG) improves the performance of semen analysis in diagnosing male infertility.

Materials And Methods: Infertile men and fertile men were recruited from the clinic. Pre- and post-DDGC values for the semen parameters of sperm concentration, total sperm number, percent total motility, percent progressive motility, percent normal sperm morphology, and sperm DNA fragmentation rate were compared.

Results: A total of 528 men (252 infertile men and 276 fertile men) were enrolled in the present study. After DDGC, sensitivity was significantly increased for sperm concentration, total sperm number, and sperm morphology (P < .01); specificity was significantly increased for progressive motility and sperm morphology (P < .01); and diagnostic accuracy was significantly improved for all of these parameters (area under the curve (AUC): P < .01). Total motility and sperm DNA fragmentation rate exhibited no obvious change in sensitivity, specificity or accuracy after DDGC (all P > .01). For the combination of all these semen parameters, diagnostic accuracy improved significantly after DDGC (AUC: P < .01). In a multiple regression analysis, only sperm morphology and sperm DNA fragmentation rate had P values less than 0.05 before DDGC, whereas all parameters except total sperm number contributed to the equation after DDGC.

Discussion: DDGC is a mature, standardized procedure for clinical commonly used to optimize spermatozoa. The diagnostic accuracy of semen analysis was significantly improved after DDGC, which indicated that assessing "functional spermatozoa" might be a more suitable method for semen analysis than the WHO 2010 criteria.

Conclusion: Assessing semen parameters after DDGC might improve their diagnostic accuracy for male infertility.
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http://dx.doi.org/10.1111/andr.12892DOI Listing
January 2021

Alteration of the fatty acid composition of Brassica napus L. via overexpression of phospholipid: Diacylglycerol acyltransferase 1 from Sapium sebiferum (L.) Roxb.

Plant Sci 2020 Sep 12;298:110562. Epub 2020 Jun 12.

Faculty of Bioscience and Biotechnology of Central South University of Forestry and Technology, 410004, Changsha, China; Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, 410018, Changsha, China. Electronic address:

Sapium sebiferum (L.) Roxb. plays an important role in traditional Chinese medicine and is one of major woody oil tree in China. Phospholipid: diacylglycerol acyltransferase 1 (PDAT1), as an important catalytic enzyme for the formation of triacylglycerol (TAG), is mainly responsible for the transfer of an acyl group from the sn-2 position of phospholipids to the sn-3 position of sn-1, 2-diacylglycerol (DAG) to produce TAG and sn-1 lysophospholipids. The importance of PDAT1 in triacylglycerol biosynthesis has been illustrated in previous research, and at least 67 PDAT1 sequences have been identified from 31 organisms. However, little is known about the gene encoding PDAT1 in S. sebiferum (SsPDAT1), which is involved in seed oil biosynthesis. To explore the functional characteristics of SsPDAT1, we cloned and analyzed the full-length cDNA in the coding region of SsPDAT1, which consists of 2040 bp and encodes a putative protein of 680 amino acid (aa) residues. Thin-layer chromatography (TLC) analysis showed that recombinant SsPDAT1 could restore TAG accumulation in TAG-deficient mutant yeast (Saccharomyces cerevisiae) H1246, which revealed the enzyme activity of SsPDAT1. Moreover, transgenic Brassica napus L. W10 plants overexpressing SsPDAT1 showed significant increases of 19.6-28.9 % in linoleic acid levels but decreases of 27.3-37.1 % in linolenic acid. Furthermore, the total oil content increased by 8.1 %-10.8 % in SsPDAT1 transgenic seeds. These results confirmed the role of SsPDAT1 in stabilizing oil biosynthesis and suggested that SsPDAT1 could be exploitable to specifically regulate the oil composition of plants. These experimental results provide a new concept that may enable the industrial development of plants with high-linoleic-acid oil through overexpression of SsPDAT1 in S. sebiferum L.
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http://dx.doi.org/10.1016/j.plantsci.2020.110562DOI Listing
September 2020

High-Resolution Radar Target Recognition via Inception-Based VGG (IVGG) Networks.

Comput Intell Neurosci 2020 18;2020:8893419. Epub 2020 Jul 18.

School of Computer and Communication Engineering, Changsha University of Science and Technology, Changsha 410114, China.

Aiming at high-resolution radar target recognition, new convolutional neural networks, namely, Inception-based VGG (IVGG) networks, are proposed to classify and recognize different targets in high range resolution profile (HRRP) and synthetic aperture radar (SAR) signals. The IVGG networks have been improved in two aspects. One is to adjust the connection mode of the full connection layer. The other is to introduce the Inception module into the visual geometry group (VGG) network to make the network structure more suik / for radar target recognition. After the Inception module, we also add a point convolutional layer to strengthen the nonlinearity of the network. Compared with the VGG network, IVGG networks are simpler and have fewer parameters. The experiments are compared with GoogLeNet, ResNet18, DenseNet121, and VGG on 4 datasets. The experimental results show that the IVGG networks have better accuracies than the existing convolutional neural networks.
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http://dx.doi.org/10.1155/2020/8893419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383303PMC
July 2020

A SAR Image Target Recognition Approach via Novel SSF-Net Models.

Comput Intell Neurosci 2020 9;2020:8859172. Epub 2020 Jul 9.

School of Computer and Communication Engineering, Changsha University of Science and Technology, Changsha 410114, China.

With the wide application of high-resolution radar, the application of Radar Automatic Target Recognition (RATR) is increasingly focused on how to quickly and accurately distinguish high-resolution radar targets. Therefore, Synthetic Aperture Radar (SAR) image recognition technology has become one of the research hotspots in this field. Based on the characteristics of SAR images, a Sparse Data Feature Extraction module (SDFE) has been designed, and a new convolutional neural network SSF-Net has been further proposed based on the SDFE module. Meanwhile, in order to improve processing efficiency, the network adopts three methods to classify targets: three Fully Connected (FC) layers, one Fully Connected (FC) layer, and Global Average Pooling (GAP). Among them, the latter two methods have less parameters and computational cost, and they have better real-time performance. The methods were tested on public datasets SAR-SOC and SAR-EOC-1. The experimental results show that the SSF-Net has relatively better robustness and achieves the highest recognition accuracy of 99.55% and 99.50% on SAR-SOC and SAR-EOC-1, respectively, which is 1% higher than the comparison methods on SAR-EOC-1.
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http://dx.doi.org/10.1155/2020/8859172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368189PMC
July 2020

Chronological age vs biological age: a retrospective analysis on age-specific serum anti-Müllerian hormone levels for 3280 females in reproductive center clinic.

Gynecol Endocrinol 2018 Oct 19;34(10):890-894. Epub 2018 Apr 19.

a Center for Reproductive Medicine , The Third Affiliated Hospital of Sun Yat-sen University , Guangzhou , People's Republic of China.

The aim of the present study was to determine the correlation between chronological age and biological age by characterizing age-specific serum anti-Müllerian hormone (AMH) values for 3280 Chinese women. A retrospective analysis including 3280 females between 10 to 52 years old was conducted from January 2016 to December 2016 in the clinical laboratory of Center for Reproductive Medicine, The Third Affiliated Hospital of Sun Yat-sen University, China. All included women were divided into several groups by age. Distribution and Statistical description of age-specific AMH levels was provided. Our results showed that serum AMH levels were negatively correlated with age (r = -0.606, p < .001). AMH concentrations approximately 31.1% depended on age and descended by an average of 6.2% per year. Around 25, 35 and 40 years, the decrease of AMH values accelerated. In conclusion, biological age was inversely correlated with chronological age. The present data can provide information for evaluating the fertility potential and ovarian reserve of infertile patients, as well as facilitate clinicians to decide individual treatment options.
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http://dx.doi.org/10.1080/09513590.2018.1462317DOI Listing
October 2018

Thawed embryo transfer and ectopic pregnancy: a meta-analysis.

Arch Gynecol Obstet 2018 06 3;297(6):1345-1352. Epub 2018 Mar 3.

Center for Reproductive Medicine, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Purpose: To examine whether thawed embryo transfers can reduce the rate of EP.

Methods: The PubMed, EMBASE, Cochrane Library databases and two randomized controlled trials registration centers were thoroughly searched until March 2017. The clinical outcomes of IVF/ICSI cycles were compared between thawed and fresh embryo transfer.

Results: Twenty-one articles were included in this meta-analysis. There were 801,464 pregnancies totally (thawed-ET: n = 158,967, fresh-ET: n = 642,497). The ectopic pregnancy rate was significantly lower in the group of thawed-ET than that in the group of fresh-ET (OR 0.69, 95% CI 0.57-0.82; I = 83%). We subdivided the data into subgroups for D3 embryo transfer and D5 embryo transfer. We also found that the ectopic pregnancy rate was significantly lower with thawed-ET on D3 than that with fresh-ET (OR 0.67, 95% CI 0.53-0.85; I = 0%). The risk of ectopic pregnancy was significantly decreased with thawed-ET on D5 than that with fresh-ET (OR 0.57, 95% CI 0.50-0.64; I = 45%).

Conclusion: Our results indicate that in contrast to fresh embryo transfers, thawed D3 or D5 embryo transfers can reduce the rate of EP.
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http://dx.doi.org/10.1007/s00404-018-4724-6DOI Listing
June 2018

Efficacy of intrauterine perfusion of granulocyte colony-stimulating factor (G-CSF) for Infertile women with thin endometrium: A systematic review and meta-analysis.

Am J Reprod Immunol 2017 08 12;78(2). Epub 2017 May 12.

Reproductive Medicine Center, The Affiliated Hospital, Guilin Medical College, Guilin, China.

This meta-analysis aimed to explore the efficiency of intrauterine perfusion of granulocyte colony-stimulating factor (G-CSF) on infertile women with thin endometrium. Following PRISMA protocol, we conducted a comprehensive search of academic literatures on various databases including PubMed, EMbase, and Cochrane Library. Studies published in English before July 1, 2016 were included for primary screening. Data on the thickness of endometrium, cycle cancelation rate,clinical pregnancy rate, and embryo implantation rate were extracted and analyzed, respectively. Eleven eligible studies involving 683 patients were included in this meta-analysis. Compared with control group, G-CSF perfusion could significantly improve endometrial thickness (mean difference [MD]=1.79, 95% confidence interval (CI): 0.92-2.67), clinical pregnancy rate (risk ratio [RR]=2.52, 95% CI: 1.39-4.55), and embryo implantation rate (RR=2.35, 95% CI: 1.20-4.60), while it could decrease cycle cancelation rate (RR=0.38, 95% CI: 0.25-0.58). Funnel plots revealed that there was no evidence of publication bias. The current data indicate that intrauterine perfusion of G-CSF can improve endometrial thickness, clinical pregnancy rate, and embryo implantation rate, but decrease the cycle cancelation rate in women with thin endometrium.
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http://dx.doi.org/10.1111/aji.12701DOI Listing
August 2017

Short versus long gonadotropin-releasing hormone analogue suppression protocols in advanced age women undergoing IVF/ICSI.

Gynecol Endocrinol 2016 Aug 18;32(8):622-624. Epub 2016 Feb 18.

b Center for Reproductive Medicine, The First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.

Objective: To compare the effective of two GnRH-a protocols for ovarian stimulation in advanced age women undergoing IVF/ICSI cycles.

Study Design: A total of 1149 IVF-ET/ICSI cycles were retrospectively identified. The cycles were divided two groups, namely a long-protocol group and a short-protocol group.

Results: The numbers of oocytes retrieved, and high-quality embryos in the long-protocol group were significantly greater than those in the short-protocol group. In the long-protocol group, the implantation and pregnancy rates were 17.22% and 33.67%, respectively, and these values were significantly higher than those in the short-protocol group (8.24% and 15.96%, p < 0.05).

Conclusions: Our study demonstrated that the long protocol was superior to the short protocol for advanced age women.
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http://dx.doi.org/10.3109/09513590.2016.1147546DOI Listing
August 2016

C14ORF166 overexpression is associated with pelvic lymph node metastasis and poor prognosis in uterine cervical cancer.

Tumour Biol 2016 Jan 29;37(1):369-79. Epub 2015 Jul 29.

Department of Gynecologic Oncology; State Key Laboratory of Oncology in South China; Collaborative Innovation Center for Cancer Medicine, Cancer Center, Sun Yat-Sen University, No. 651, Dongfeng Road East, Guangzhou, 510060, People's Republic of China.

C14ORF166 (chromosome 14 open reading frame 166) is a transcriptional repressor related to the regulation of centrosome architecture. However, the role of C14ORF166 in the development and progression of cancer remains largely unknown. The aim of this study was to investigate the expression and clinicopathological significance of C14ORF166 in cervical cancer. C14ORF166 expression was analyzed using quantitative real-time PCR (RT-PCR) and Western blotting in cervical cancer cell lines and eight paired cervical cancer samples and the adjacent normal tissues. Immunohistochemistry was used to analyze C14ORF166 protein expression in 148 clinicopathologically characterized cervical cancer specimens. Statistical analyses were performed to evaluate the relationship between the expression of C14ORF166 and clinicopathologic features and prognosis. C14ORF166 mRNA and protein expression were significantly upregulated in cervical cancer cell lines and tissue samples (P < 0.05). Immunohistochemical analysis revealed a high expression of C14ORF166 was observed in 39.9 % (59/148) of the cervical cancer specimens; the remaining samples expressed low levels or did not express any detectable C14ORF166. The chi-square test indicated that high-level expression of C14ORF166 was significantly associated with International Federation of Gynecology and Obstetrics (FIGO) stage (P < 0.001), vital status (P = 0.026), tumor size (P = 0.034), serum squamous cell carcinoma antigen level (SCC-Ag; P = 0.035), and pelvic lymph node metastasis (P < 0.001). Patients with highly expressed C14ORF166 showed a tendency to receive postoperative chemotherapy (P = 0.005) and postoperative radiation (P = 0.008). Furthermore, high C14ORF166 expression was associated with poorer overall survival compared to low C14ORF166 expression, and C14ORF166 was a significant prognostic factor in univariate and multivariate analysis (P < 0.05). High C14ORF166 expression had prognostic value for poor outcome in cervical cancer. C14ORF166 may represent a biomarker of pelvic lymph node metastasis and enable the identification of high-risk patients along with selection of appropriate treatment strategies.
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http://dx.doi.org/10.1007/s13277-015-3806-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841849PMC
January 2016

Short versus Long Gonadotropin-Releasing Hormone Analogue Suppression Protocols in IVF/ICSI Cycles in Patients of Various Age Ranges.

PLoS One 2015 24;10(7):e0133887. Epub 2015 Jul 24.

Center for Reproductive Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Objective: To compare the two GnRH-a protocols (long GnRH-a protocol and short GnRH-a protocol) for ovarian stimulation in IVF/ICSI cycles in patients of various age ranges.

Methods: A total of 5662 IVF-ET/ICSI cycles from 2010 to 2013 were retrospectively identified. The cycles were divided into two groups: a long protocol group and short protocol group. In each group, the patients were divided into four age ranges: <31 years, 31 to 35 years, 36 to 40 years, and >40 years. The duration of stimulation, total dose of Gn, implantation rate and pregnancy rate were compared.

Results: The total dose of Gn was significantly higher, and the duration of stimulation was significantly longer, in the long protocol group than in the short protocol group for all age ranges (P<0.05). If the patients were of the same age range, the number of oocytes retrieved, MII oocytes, and high-quality embryos in the long protocol group were all significantly greater than those in the short protocol group (P<0.05). In the long protocol group, the clinical pregnancy rates of the four age ranges were 52.76%, 44.33%, 36.15% and 13.33%, respectively, which were significantly higher than those in the short protocol group (33.33%, 24.58%, 22.49% and 8.72%, respectively; P<0.05). The same trend was also found in the implantation rates of the four age ranges. As the age increased, the clinical pregnancy and implantation rates, as well as the number of oocytes retrieved, MII oocytes, and high-quality embryos, of the long protocol group significantly decreased (P<0.05).

Conclusions: Our study demonstrated that regardless of patient age, the long protocol was superior to the short protocol in terms of the number of retrieved oocytes, as well as the implantation and pregnancy rates.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133887PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514806PMC
May 2016

Comparison of fertilization outcome between microdrop and open insemination methods in non-male factor IVF patients.

Syst Biol Reprod Med 2014 Jun 5;60(3):165-70. Epub 2014 Feb 5.

The First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.

Both microdrop and open methods are commonly used for in vitro fertilization (IVF) protocols for embryo culture as well as oocyte insemination. However, few comparative studies evaluating the microdrop or open method of insemination on the fertilization outcome and subsequent embryo development have been performed. A randomized study was conducted to compare microdrop and open fertilization with respect to fertilization rate and embryo development among non-male factor patients undergoing in vitro fertilization and embryo transfer (IVF-ET). The results presented in this study demonstrate that the fertilization failure rate [total fertilization failure rate (TFF) plus low fertilization rate (<25% oocytes fertilized)] in the microdrop insemination group was higher than in the open insemination group (11.9% versus 3.3%, p < 0.001), while the good quality embryo rate and pregnancy rate did not differ significantly between the groups. As a highly complicated process involving many extrinsic and intrinsic factors, further studies are needed to confirm the effects of these insemination methods on the rate of fertilization failure.
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http://dx.doi.org/10.3109/19396368.2013.872707DOI Listing
June 2014

[Acute fluoroacetamide poisoning with main damage to the heart].

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2002 Oct;20(5):344-6

Department of Special Examination, Guangzhou No. 12 Hospital, Guangzhou 510620, China.

Objective: To observe the heart damage in 10 patients with acute fluoroacetamide poisoning.

Methods: Monitoring serum activities of myocardial enzymes [creatine kinase (CK), asparate aminotransferase (AST), lactate dehydrogenase(LDH) and hydroxybutyrate dehydrogenase(HBDH)] and recording ECG on these 10 patients were performed during the period of their hospitalization. In the mean while, 24 hour dynamic ECG were also recorded and analysed using GP7000L Holter.

Results: (1) Urinary fluorine ion concentrations were increased in 9 patients before therapy and in all these 10 patients during therapeutic period. (2) The activities of serum CK in 2 patients and that of serum HBDH in one patient were increased before therapy. However, the serum activities of one or more than one myocardial enzymes were increased in all these 10 patients during therapeutic period. (3) Four patients had abnormal change of ECG before therapy and 5 patients during therapeutic period. (4) 24 hour dynamic ECG records showed that there were heart electrical alternans in 9 patients. One patient had wandering pacemaker and 6 patients had arythmia.

Conclusion: Fluoroacetamide may cause obvious heart damage, and also heart electrical alternation.
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October 2002