Publications by authors named "Jianping Li"

598 Publications

Egg consumption associated with all-cause mortality in rural China: a 14-year follow-up study.

Eur J Public Health 2021 May 5. Epub 2021 May 5.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.

Background: Dietary recommendations regarding egg intake remain controversial topic for public health. We hypothesized that there was a positive association between egg consumption and all-cause mortality.

Methods: To test this hypothesis, we enrolled 9885 adults from a community-based cohort in Anhui Province, China during 2003-05. Egg consumption was assessed by food questionnaire. Stratified analyses were performed for age, sex, body mass index (BMI), blood pressure, smoking, drinking and laboratory tests.

Results: After an average follow-up of 14.1 years, 9444 participants were included for analysis. A total of 814 deaths were recorded. Participants' BMI and lipid profile had no significantly difference between three egg consumption groups. BMI was 21.6±2.7 of the whole population, especially BMI>24 was only 17.3%. A bivariate association of egg consumption >6/week with increased all-cause mortality was observed compared with ≤6/week (RR: 1.35, 95% CI: 1.05, 1.73, P = 0.018). A significant interaction was observed for BMI ≥ 21.2 kg/m2 vs. BMI<21.2 kg/m2 (P for interaction: 0.001). No other significant interactions were found.

Conclusions: In this study, consuming >6 eggs/week increased risk of all-cause mortality, even among lean participants, especially who with BMI ≥ 21.2 kg/m2. Eggs are an easily accessible and constitute an affordable food source in underdeveloped regions. Consuming <6 eggs/week may be the most suitable intake mode.
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http://dx.doi.org/10.1093/eurpub/ckaa250DOI Listing
May 2021

Protective effect of glutamine and alanyl-glutamine against zearalenone-induced intestinal epithelial barrier dysfunction in IPEC-J2 cells.

Res Vet Sci 2021 Apr 24;137:48-55. Epub 2021 Apr 24.

Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China. Electronic address:

Zearalenone (ZEN), a nonsteroidal estrogenic mycotoxin, has a negative effect on porcine intestine. Glutamine (Gln) and alanyl-glutamine (Ala-Gln) are nutrients with potential preservation functions similar to those of the intestinal epithelial barrier. The protective role of Gln and Ala-Gln on ZEN-induced intestinal barrier dysfunction was evaluated in this study. Additionally, the ability of Gln and Ala-Gln to protect the intestinal barrier was investigated. Our results showed that lactate dehydrogenase (LDH) activity, paracellular permeability and reactive oxygen species (ROS) level were increased by ZEN, while the glutathione (GSH) level was decreased by ZEN. Gln and Ala-Gln promoted the proliferation of cells and attenuated the ZEN-induced increase in cytotoxicity, cell apoptosis and paracellular permeability. Gln and Ala-Gln alleviated barrier function damage, which was additionally induced by ZEN by increasing the antioxidant capacity of cells. In addition, Gln and Ala-Gln upregulated intestinal barrier associated gene expressions including pBD-1, pBD-2, MUC-2, ZO-1, occludin and claudin-3. This study revealed that Gln and Ala-Gln had similar effects in protecting intestinal epithelial barrier function against ZEN exposure in IPEC-J2 cells. A new treatment for alleviating ZEN-induced injury to the intestine through nutritional intervention is provided.
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http://dx.doi.org/10.1016/j.rvsc.2021.04.027DOI Listing
April 2021

A Strip Adjustment Method of UAV-Borne LiDAR Point Cloud Based on DEM Features for Mountainous Area.

Sensors (Basel) 2021 Apr 15;21(8). Epub 2021 Apr 15.

State Key Laboratory of Information Engineering in Surveying, Mapping and Remote Sensing, Wuhan University, Wuhan 430079, China.

Due to the trajectory error of the low-precision position and orientation system (POS) used in unmanned aerial laser scanning (ULS), discrepancies usually exist between adjacent LiDAR (Light Detection and Ranging) strips. Strip adjustment is an effective way to eliminate these discrepancies. However, it is difficult to apply existing strip adjustment methods in mountainous areas with few artificial objects. Thus, digital elevation model-iterative closest point (DEM-ICP), a pair-wise registration method that takes topography features into account, is proposed in this paper. First, DEM-ICP filters the point clouds to remove the non-ground points. Second, the ground points are interpolated to generate continuous DEMs. Finally, a point-to-plane ICP algorithm is performed to register the adjacent DEMs with the overlapping area. A graph-based optimization is utilized following DEM-ICP to estimate the correction parameters and achieve global consistency between all strips. Experiments were carried out using eight strips collected by ULS in mountainous areas to evaluate the proposed method. The average root-mean-square error (RMSE) of all data was less than 0.4 m after the proposed strip adjustment, which was only 0.015 m higher than the result of manual registration (ground truth). In addition, the plane fitting accuracy of lateral point clouds was improved 4.2-fold, from 1.565 to 0.375 m, demonstrating the robustness and accuracy of the proposed method.
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http://dx.doi.org/10.3390/s21082782DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071264PMC
April 2021

HLA-A2.1-restricted ECM1-derived epitope LA through DC cross-activation priming CD8 T and NK cells: a novel therapeutic tumour vaccine.

J Hematol Oncol 2021 Apr 28;14(1):71. Epub 2021 Apr 28.

Department of Pharmacology, School of Pharmacy, China Medical University, No. 13, Beihai Road, Dadong District, Shenyang, Liaoning Province, China.

Background: CD8 T cell-mediated adaptive cellular immunity and natural killer (NK) cell-mediated innate immunity both play important roles in tumour immunity. This study aimed to develop therapeutic tumour vaccines based on double-activation of CD8 T and NK cells.

Methods: The immune Epitope database, Molecular Operating Environment software, and enzyme-linked immunosorbent assay were used for epitope identification. Flow cytometry, confocal microscopy, UPLC-QTOF-MS, and RNA-seq were utilized for evaluating immunity of PBMC-derived DCs, CD8 T or NK cells and related pathways. HLA-A2.1 transgenic mice combined with immunologically reconstituted tumour-bearing mice were used to examine the antitumour effect and safety of epitope vaccines.

Results: We identified novel HLA-A2.1-restricted extracellular matrix protein 1(ECM1)-derived immunodominant epitopes in which LA induced a potent immune response. We also found that LA-loaded DCs upregulated the frequency of CD3/CD8 T cells, CD45RO/CD69 activated memory T cells, and CD3/CD16/CD56 NK cells. We demonstrated cytotoxic granule release of LA/DC-CTLs or LA/DC-NK cells and cytotoxicity against tumour cells and microtissue blocks via the predominant IFN-γ/perforin/granzyme B cell death pathway. Further investigating the mechanism of LA-mediated CD8 T activation, we found that LA could be internalized into DCs through phagocytosis and then formed a LA-MHC-I complex presented onto the DC surface for recognition of the T cell receptor to upregulate Zap70 phosphorylation levels to further activate CD8 T cells by DC-CTL interactions. In addition, LA-mediated DC-NK crosstalk through stimulation of the TLR4-p38 MAPK pathway increased MICA/B expression on DCs to interact with NKG2D for NK activation. Promisingly, LA could activate CD8 T cells and NK cells simultaneously via interacting with DCs to suppress tumours in vivo. Moreover, the safety of LA was confirmed.

Conclusions: LA-induced immune antitumour activity through DC cross-activation with CD8 T and NK cells, which demonstrated proof-of-concept evidence for the capability and safety of a novel therapeutic tumour vaccine.
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http://dx.doi.org/10.1186/s13045-021-01081-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082934PMC
April 2021

Protection against SARS-CoV-2 infection by a mucosal vaccine in rhesus macaques.

JCI Insight 2021 Apr 28. Epub 2021 Apr 28.

Vaccine Branch, Center for Cancer Research, NCI, NIH, Bethesda, United States of America.

Effective SARS-CoV-2 vaccines are urgently needed. While most vaccine strategies have focused on systemic immunization, here we compared the protective efficacy of two adjuvanted subunit vaccines with spike protein S1: an intramuscular (IM)- primed /boosted vaccine and an IM-primed/intranasal (IN)-boosted mucosal vaccine, in rhesus macaques. The IM-alum-only vaccine induced robust binding and neutralizing antibody and persistent cellular immunity systemically and mucosally, while IN boosting with nanoparticles including IL-15 and TLR agonists elicited weaker T-cell and antibody responses, but higher dimeric IgA and IFNa. Nevertheless, following SARS-CoV-2 challenge, neither group showed detectable subgenomic RNA in upper or lower respiratory tracts vs naïve controls, indicating full protection against viral replication. Though mucosal and systemic protective mechanisms may differ, results demonstrate both vaccines can protect against respiratory SARS-CoV-2 exposure. The mucosal vaccine was safe after multiple doses and cleared the input virus more efficiently in the nasal cavity, and thus may act as a potent complementary reinforcing boost for conventional systemic vaccines to provide overall better protection.
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http://dx.doi.org/10.1172/jci.insight.148494DOI Listing
April 2021

Rapid pH-responsive self-disintegrating nanoassemblies balance tumor accumulation and penetration for enhanced anti-breast cancer therapy.

Acta Biomater 2021 Apr 18. Epub 2021 Apr 18.

Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, People's Republic of China. Electronic address:

The dilemma of tumor accumulation and deep penetration has always been a barrier in antitumor therapy. Stimuli-responsive size changeable drug delivery systems provide possible solutions. Nevertheless, the low size-shrinkage efficiency limited the antitumor effects. In this study, an instant pH-responsive size shrinkable nanoassemblies named self-aggregated DOX@HA-CD (SA-DOX@HA-CD) was formulated using small-sized hyaluronic acid modified carbon dots (HA-CD) as monomers, which could self-aggregate into raspberry-like structure via hydrophobicity force in neutral pH and rapidly disassemble into shotgun-like DOX-loaded CD monomer in simulated tumor microenvironment (pH 6.5), owing to the transformation in electrical charge and hydrophobicity/hydrophilicity of this system. The transmission electron microscopy showed that the clustered SA-DOX@HA-CD had a diameter of ∼ 150 nm, and thoroughly disassembled into ∼30 nm nanoparticles in response to acidic environment. The disassemble efficiency was approximately 100%. Attributed to this property, SA-DOX@HA-CD led to enhanced cellular internalization and accumulation in 4T1 cells in simulated tumor microenvironment, as well as deep tumor penetration in 3D tumor spheroid model. Besides, the imine bond between DOX and HA-CD endowed DOX with pH-responsive release profile in the acidic lysosome environment. Furthermore, in the orthotopic 4T1 tumor-bearing mouse model, SA-DOX@HA-CD demonstrated higher tumor accumulation than non-aggregated DOX-HA-CD. Meanwhile, in response to the acid tumor microenvironment, the dissociated DOX-HA achieved deep tumor penetration, which consequently resulted in 2.5-fold higher antitumor efficiency. The formulation of self-aggregated SA-DOX@HA-CD provides a simple and effective alternative to prepare pH-responsive size-shrinkable nanodrug delivery systems. Statement of Significance: The heterogeneity of tumor vasculature and the high tumor interstitial pressure lead to the barriers in tumor accumulation and deep penetration, which calls for opposite properties (e.g. size) of drug delivery systems. To address this dilemma, various size changeable nanoparticles have been developed utilizing special features of tumor microenvironment, such as pH, enzyme and reactive oxygen species. Nevertheless, the current strategies face the problems of incomplete hydrolysis of chemical bonds or insufficient enzyme degradation, which result in only partial size shrinkage, hindering the tumor deep penetration effects. Here we developed a self-assembled nanocluster, which could respond to acidic pH rapidly and thoroughly disassemble into small nanodots due to the alteration of hydrophobicity/hydrophilicity/charge, leading to approximately 100% dissociation. This strategy provides a new concept for design of size changeable drug delivery systems.
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http://dx.doi.org/10.1016/j.actbio.2021.04.022DOI Listing
April 2021

Peptide-based therapeutic cancer vaccine: Current trends in clinical application.

Cell Prolif 2021 May 22;54(5):e13025. Epub 2021 Mar 22.

Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, China.

The peptide-based therapeutic cancer vaccines have attracted enormous attention in recent years as one of the effective treatments of tumour immunotherapy. Most of peptide-based vaccines are based on epitope peptides stimulating CD8 T cells or CD4 T helper cells to target tumour-associated antigens (TAAs) or tumour-specific antigens (TSAs). Some adjuvants and nanomaterials have been exploited to optimize the efficiency of immune response of the epitope peptide to improve its clinical application. At present, numerous peptide-based therapeutic cancer vaccines have been developed and achieved significant clinical benefits. Similarly, the combination of peptide-based vaccines and other therapies has demonstrated a superior efficacy in improving anti-cancer activity. We delve deeper into the choices of targets, design and screening of epitope peptides, clinical efficacy and adverse events of peptide-based vaccines, and strategies combination of peptide-based therapeutic cancer vaccines and other therapies. The review will provide a detailed overview and basis for future clinical application of peptide-based therapeutic cancer vaccines.
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http://dx.doi.org/10.1111/cpr.13025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088465PMC
May 2021

A sensitive electrochemiluminescence DNA biosensor based on the signal amplification of ExoIII enzyme-assisted hybridization chain reaction combined with nanoparticle-loaded multiple probes.

Mikrochim Acta 2021 Mar 15;188(4):125. Epub 2021 Mar 15.

Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, Guangxi, China.

An electrochemiluminescence (ECL) DNA biosensor based on ExoIII exonuclease assistance and hybridization chain reaction (HCR) amplification technology has been constructed. ExoIII exonuclease and triple-helix DNA molecular switch are used in detecting a target in circulation. By combining HCR with AuNPs@DNA, a novel signal probe is built, which enables multiple signal amplification and the high-sensitive detection of transgenic rice BT63 DNA. The FeO@Au solution is added to a magneto-controlled glassy carbon electrode, and sulfhydryl-modified capture DNA (CP) is immobilized on FeO@Au through the Au-S bond. Mercaptoethanol is added to close sites and prevent the nonspecific adsorption of CP on the magnetron glassy carbon electrode. A target DNA is added to a constructed triple-helix DNA molecular centrifuge tube for reaction. Owing to base complementation and the reversible switching of the triple-helix DNA molecular state, the target DNA turns on the triple-helix DNA molecular switch and hybridizes with a long-strand recognition probe (RP) to form a double-stranded DNA (dsDNA). Exonuclease ExoIII is added to specifically recognize and cut the dsDNA and to release the target DNA. The target DNA strand then circulates back completely to open the multiple triple-helix DNA molecular switch, releasing a large number of signal transduction probes (STP). To hybridize with CP, a large amount of STP is added to the electrode. Finally, a AuNPs@DNA signal probe is added to hybridize with STP. H1 and H2 probes are added for the hybridization chain reaction and the indefinite extension of the primer strand on the probe. Then, tris-(bipyridyl)ruthenium(II) is added for ECL signal detection with PBS-tri-n-propylamine as the base solution. In the concentration range 1.0 × 10 to 1.0 × 10 mol/L of the target DNA, good linear relationship was achieved with the corresponding ECL signal. The detection limit is 3.6 × 10 mol/L. The spiked recovery of the rice samples range from 97.2 to 101.5%. The sensor is highly sensitive and has good selectivity, stability, and reproducibility. A novel electrochemiluminescence biosensor with extremely higher sensitivity was prepared for the determination of ultra-trace amount transgenic rice BT63 DNA. The sensitivity was significantly improved by multiple signal enhancements. Firstly, a large number of signal transduction probes are released when the triple-helix DNA molecular switch unlock after recycles assisted by ExoIII exonuclease under target BT63 DNA; and then the signal transduction probes hybridize with the signal probes of AuNPs@(DNA-HCR) produced through hybridization chain reaction. Finally, the signal probes which were embedded with a large amount of electrochemiluminescence reagent produce high luminescence intensity. The detection limit was 3.6 × 10 mol/L, which is almost the most sensitive methods reported.
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http://dx.doi.org/10.1007/s00604-021-04777-2DOI Listing
March 2021

The effect of soil depth on temperature sensitivity of extracellular enzyme activity decreased with elevation: Evidence from mountain grassland belts.

Sci Total Environ 2021 Mar 2;777:146136. Epub 2021 Mar 2.

Department of Ecology, College of Urban and Environmental Sciences and Key Laboratory for Earth Surface Processes of the Ministry of Education, Peking University, Beijing 100871, China. Electronic address:

Temperature sensitivity of soil extracellular enzyme activity (EEA), indicated by the temperature coefficient Q, is used to predict the effect of temperature on soil carbon (C), nitrogen (N), and phosphorus (P) cycling. At present, we lack understanding of elevation and soil depth variations in Q of EEA. Here, we measured the Q of three enzymes participating in C- (β-1,4-glucosidase, BG), N- (leucine aminopeptidase, LAP), and P- (acid phosphatase, AP) cycling along a vertical grassland belt of China. Soils from five depths (0-10, 10-20, 20-40, 40-60, and 60-100 cm) were sampled from three elevations (low, <1000 m; middle, 1000-2000 m; high, 2000-3000 m) and incubated at four temperatures (5, 15, 25, 35 °C). The average Q of soil EEA ranged from 0.97 to 1.11 and the Q of LAP was higher than that of BG and AP. Generally, the Q of BG and LAP both increased from low to middle elevation and then decreased, while the Q of AP was stable. Moreover, the effect of soil depth on Q of EEA was weakened from low elevation to high elevation, and the factors driving Q of soil EEA changed with elevation. This study improved the understanding of the vertical pattern of Q of soil EEA in water-limited ecosystems, and highlighted that elevation could regulate the effect of soil depth on Q of EEA.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146136DOI Listing
March 2021

Pelleting of a Total Mixed Ration Affects Growth Performance of Fattening Lambs.

Front Vet Sci 2021 12;8:629016. Epub 2021 Feb 12.

The Innovation Centre of Ruminant Precision Nutrition and Smart and Ecological Farming, Jilin Agricultural Science and Technology University, Jilin City, China.

Feeding pelleted total mixed rations (TMR) instead of traditional loose concentrate plus forage to fattening lambs is an emerging practice. This study aimed to determine the effects of feeding pelleted TMR to fattening lambs on feed intake behaviour, growth performance, feed digestion, rumen fermentation characteristics, rumen microbial community, serum parameters, slaughter performance, meat quality, and the economic outcome. Two physical forms (pelleted vs. un-pelleted) of TMR composed of the same ingredients with the same particle sizes were compared in three animal experiments. Feed intake and average daily gain were higher when the TMR was pelleted, but apparent total tract digestibility of nutrients (organic matter, crude protein, neutral detergent fibre, acid detergent fibre, and ether extract) and serum parameters were not affected and apparent total tract dry matter digestibility was slightly lower. Feeding pelleted TMR increased total short-chain fatty acid concentration and decreased rumen pH. Rumen microbial community was not affected by the physical form of the TMR at phylum level but changed slightly at genus level. Liveweight at slaughter and hot carcass weight were higher for lambs fed the pelleted compared to the un-pelleted TMR, while dressing percentage and meat quality were not affected. In conclusion, feeding pelleted TMR improves growth performance of fattening lambs mainly due to an increase in feed intake. Feeding pelleted TMR is a feasible strategy for intensive lamb fattening operations.
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http://dx.doi.org/10.3389/fvets.2021.629016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928353PMC
February 2021

Short-term nitrogen dioxide exposure is associated with the spread of S. pyogenes-induced vulvovaginitis in prepubertal girls in Hangzhou, China.

Environ Sci Pollut Res Int 2021 Mar 6. Epub 2021 Mar 6.

Department of Clinical Laboratory, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Binsheng Road, Hangzhou, 3333, Zhejiang Province, China.

As a cause of vulvovaginitis in prepubertal girls, Streptococcus pyogenes (S. pyogenes) is commonly isolated from vaginal introitus swabs. Studies have identified several risk factors, but have not focused on the correlation between ambient air pollutants and S. pyogenes-induced vulvovaginitis in prepubertal girls. This study was conducted to determine whether ambient air pollutants were associated with S. pyogenes-induced vulvovaginitis in prepubertal girls. Daily data about S. pyogenes-induced vulvovaginitis in prepubertal girls from the outpatient department of Children's Hospital at the Zhejiang University School of Medicine in Hangzhou City between January 1, 2015, and December 31, 2018, were retrospectively reviewed. Ambient air pollutants in Hangzhou were measured daily. A generalized additive model (GAM) was utilized to assess the associations between daily air pollutants and S. pyogenes isolates obtained from vaginal introitus swabs of prepubertal girls. The mean daily concentration of nitrogen dioxide (NO) in Hangzhou City during the study period was 44.6 μg/m (25th-75th percentiles, 32.0-56.0 μg/m). The GAM showed that the largest estimate effects in S. pyogenes-induced vulvovaginitis in prepubertal girls were found in NO with a moving (accumulative) average on day 3. The excess risk of NO in terms of the daily number of S. pyogenes isolates obtained from the vaginal introitus swabs was 14.91% (95% confidence interval [CI]: 4.85-25.94%) in the single-pollutant model. The multipollutant model revealed that an increase of 10 μg/m in NO exposure was associated with an 18.33% increased risk for acquiring S. pyogenes-induced vulvovaginitis in prepubertal girls (95% CI: 1.21-38.35%; P < 0.05). In conclusion, short-term NO exposure was strongly associated with the spread of S. pyogenes-induced vulvovaginitis in prepubertal girls.
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http://dx.doi.org/10.1007/s11356-021-13268-zDOI Listing
March 2021

Structure of membrane diacylglycerol kinase in lipid bilayers.

Commun Biol 2021 Mar 5;4(1):282. Epub 2021 Mar 5.

National Center for Magnetic Resonance in Wuhan, Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, 430071, People's Republic of China.

Diacylglycerol kinase (DgkA) is a small integral membrane protein, responsible for the ATP-dependent phosphorylation of diacylglycerol to phosphatidic acid. Its structures reported in previous studies, determined in detergent micelles by solution NMR and in monoolein cubic phase by X-ray crystallography, differ significantly. These differences point to the need to validate these detergent-based structures in phospholipid bilayers. Here, we present a well-defined homo-trimeric structure of DgkA in phospholipid bilayers determined by magic angle spinning solid-state NMR (ssNMR) spectroscopy, using an approach combining intra-, inter-molecular paramagnetic relaxation enhancement (PRE)-derived distance restraints and CS-Rosetta calculations. The DgkA structure determined in lipid bilayers is different from the solution NMR structure. In addition, although ssNMR structure of DgkA shows a global folding similar to that determined by X-ray, these two structures differ in monomeric symmetry and dynamics. A comparative analysis of DgkA structures determined in three different detergent/lipid environments provides a meaningful demonstration of the influence of membrane mimetic environments on the structure and dynamics of membrane proteins.
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http://dx.doi.org/10.1038/s42003-021-01802-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935881PMC
March 2021

Quantitative Evaluation of Burn Injuries Based on Electrical Impedance Spectroscopy of Blood with a Seven-Parameter Equivalent Circuit.

Sensors (Basel) 2021 Feb 21;21(4). Epub 2021 Feb 21.

Graduate School of Mechanical Engineering, Division of Artificial System Science, Chiba University, 1-33 Yayoi, Inage, Chiba 263-8522, Japan.

A quantitative and rapid burn injury detection method has been proposed based on the electrical impedance spectroscopy (EIS) of blood with a seven-parameter equivalent circuit. The degree of burn injury is estimated from the electrical impedance characteristics of blood with different volume proportions of red blood cells (RBCs) and heated red blood cells (HRBCs). A quantitative relationship between the volume portion of HRBCs and the electrical impedance characteristics of blood has been demonstrated. A seven -parameter equivalent circuit is employed to quantify the relationship from the perspective of electricity. Additionally, the traditional Hanai equation has been modified to verify the experimental results. Results show that the imaginary part of impedance under the characteristic frequency () has a linear relationship with which could be described by = -2.56 - 2.01 with a correlation coefficient of 0.96. Moreover, the relationship between the plasma resistance and is obtained as = -7.2 + 3.91 with a correlation coefficient of 0.96 from the seven -parameter equivalent circuit. This study shows the feasibility of EIS in the quantitative detection of burn injury by the quantitative parameters and , which might be meaningful for the follow-up clinical treatment for burn injury.
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http://dx.doi.org/10.3390/s21041496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926917PMC
February 2021

ClC-5 Downregulation Induces Osteosarcoma Cell Apoptosis by Promoting Bax and tBid Complex Formation.

Front Oncol 2020 5;10:556908. Epub 2021 Feb 5.

Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China.

Osteosarcoma is the most common malignant bone tumor. Chloride (Cl) channels-mediated Cl movement plays an important role in regulating the functions of various cancer cells, but its role in osteosarcoma remains unclear. In this study, we found that ClC-5 was increased in osteosarcoma tissues compared with normal bone tissues. Patients with high ClC-5 expression showed poor overall survival relative to those patients with low ClC-5 expression. Higher ClC-5 expression and lower intracellular Cl concentration ([Cl]) were observed in osteosarcoma cells compared with normal osteoblasts. Lowering [Cl] increased the viability of osteosarcoma cells, which was markedly blocked by ClC-5 downregulation. Knockdown of ClC-5 significantly induced osteosarcoma cell apoptosis and increased the release of cytochrome c from mitochondria to cytosol, concomitantly with cleavage of caspase-9, caspase-3, and PARP. The effect of ClC-5 downregulation on osteosarcoma cell apoptosis and viability was abolished by caspase-3 and caspase-9 inhibitors, but not caspase-8 inhibitor. Furthermore, ClC-5 inhibition promoted Bax translocation from cytosol to mitochondria. Immunoprecipitation showed that ClC-5 interacted with Bax and ClC-5 downregulation enhanced Bax and tBid complex formation. Collectively, we demonstrate that ClC-5 downregulation induces osteosarcoma cell apoptosis mitochondria-dependent apoptotic pathway activation by promoting Bax and tBid association and subsequent Bax translocation.
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http://dx.doi.org/10.3389/fonc.2020.556908DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892965PMC
February 2021

Increased precipitation enhances soil respiration in a semi-arid grassland on the Loess Plateau, China.

PeerJ 2021 2;9:e10729. Epub 2021 Feb 2.

School of Agriculture, Ningxia University, Yinchuan, Ningxia, China.

Background: Precipitation influences the vulnerability of grassland ecosystems, especially upland grasslands, and soil respiration is critical for carbon cycling in arid grassland ecosystems which typically experience more droughty conditions.

Methods: We used three precipitation treatments to understand the effect of precipitation on soil respiration of a typical arid steppe in the Loess Plateau in north-western China. Precipitation was captured and relocated to simulate precipitation rates of 50%, 100%, and 150% of ambient precipitation.

Results And Discussion: Soil moisture was influenced by all precipitation treatments. Shoot biomass was greater, though non-significantly, as precipitation increased. However, both increase and decrease of precipitation significantly reduced root biomass. There was a positive linear relationship between soil moisture and soil respiration in the study area during the summer (July and August), when most precipitation fell. Soil moisture, soil root biomass, pH, and fungal diversity were predictors of soil respiration based on partial least squares regression, and soil moisture was the best of these.

Conclusion: Our study highlights the importance of increased precipitation on soil respiration in drylands. Precipitation changes can cause significant alterations in soil properties, microbial fungi, and root biomass, and any surplus or transpired moisture is fed back into the climate, thereby affecting the rate of soil respiration in the future.
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http://dx.doi.org/10.7717/peerj.10729DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7863787PMC
February 2021

Coronary angiography-derived contrast fractional flow reserve.

Catheter Cardiovasc Interv 2021 Feb 15. Epub 2021 Feb 15.

Department of Cardiology, Peking University First Hospital, Beijing, China.

Background: Based on coronary angiography and mean aortic pressure, a specially designed computational flow dynamics (CFD) method is proposed to determine contrast fractional flow reserve (cFFR) without using invasive pressure wire. This substudy assessed diagnostic performance of coronary angiography-derived cFFR in catheterization laboratory, based on a previous multicenter trial for online assessment of coronary angiography-derived FFR (caFFR).

Methods: Patients with diagnosis of stable angina pectoris or unstable angina pectoris were enrolled in six centers. Wire-based FFR was measured in coronary arteries with 30-90% diameter stenosis. Offline angiography-derived cFFR was computed in blinded fashion against the wire-based FFR and caFFR at an independent core laboratory.

Results: A total of 330 patients were enrolled to fulfill inclusion/exclusion criteria from June 26 to December 18, 2018. Offline angiography-derived cFFR and wire-based FFR results were compared in 328 interrogated vessels. The statistical analysis showed the highest diagnostic accuracy of 89.0 and 86.6% for angiography-derived cFFR with a cutoff value of 0.94 and 0.93 against the wire-based FFR with a cutoff value of 0.80 and 0.75, respectively. The corresponding sensitivity and specificity were 92.2 and 87.3% for the cutoff value of 0.94 and 80.0 and 88.4% for the cutoff value of 0.93, which are similar to those against the caFFR. The receiver-operating curve has area under the curve of 0.951 and 0.972 for the wire-based FFR with the cutoff value of 0.80 and 0.75, respectively.

Conclusions: Coronary angiography-derived cFFR showed higher accuracy, sensitivity, and specificity against wired-based FFR and caFFR. Hence, angiography-derived cFFR could enhance the hemodynamic assessment of coronary lesions.
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http://dx.doi.org/10.1002/ccd.29558DOI Listing
February 2021

Neutrophil counts and the risk of first stroke in general hypertensive adults.

Hypertens Res 2021 Feb 9. Epub 2021 Feb 9.

National Clinical Research Center for Kidney Disease, State Key Laboratory for Organ Failure Research, Division of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.

We aimed to investigate the association between neutrophil counts and first stroke and examine possible effect modifiers among treated hypertensive adults. This is a post hoc analysis of the China Stroke Primary Prevention Trial (CSPPT). A total of 11,878 hypertensive adults with data on neutrophil counts at baseline were included in the current study. The primary outcome was first stroke. During a median follow-up of 4.5 years, 414 (3.5%) participants experienced a first stroke, including 358 with ischemic stroke, 55 with hemorrhagic stroke and one with uncertain type of stroke. Compared with participants in quartile 1 (<2.9 × 10/L) of neutrophil counts, those in the upper quartiles (quartile 2-4 [≥2.9 × 10/L]) had a significantly higher risk of first stroke (HR, 1.35; 95% CI: 1.02, 1.78) or first ischemic stroke (HR, 1.38; 95% CI: 1.02, 1.86). Moreover, a strong positive association between neutrophil counts and first ischemic stroke was found in participants with total homocysteine (tHcy) levels <15 μmol/L (HR, 1.74; 95% CI: 1.17, 2.58; vs. ≥15 μmol/L; HR, 0.91; 95% CI: 0.57, 1.46, P interaction = 0.042) at baseline or time-averaged mean arterial pressure (MAP) ≥102 mmHg (median) (HR, 1.92; 95% CI: 1.27, 2.89; vs. <102 mmHg; HR, 0.89; 95% CI: 0.57, 1.41, P interaction = 0.015) during the treatment period. However, no such association between neutrophil counts and first hemorrhagic stroke was found. In summary, high baseline neutrophil counts were associated with an increased risk of first ischemic stroke among hypertensive patients, especially in those with low tHcy at baseline or high time-averaged MAP during the treatment period.
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http://dx.doi.org/10.1038/s41440-021-00625-1DOI Listing
February 2021

Lipid profiles and the risk of new-onset hypertension in a Chinese community-based cohort.

Nutr Metab Cardiovasc Dis 2021 03 5;31(3):911-920. Epub 2020 Dec 5.

Department of Cardiology, Peking University First Hospital, Beijing, China. Electronic address:

Background And Aims: Dyslipidemia and hypertension, key risk factors for cardiovascular disease, may share similar pathophysiological processes. A longitudinal association was reported between dyslipidemia and new-onset hypertension, but few data were published in Asian. We aimed to investigate the association of lipid profiles with new-onset hypertension in a Chinese community-based non-hypertensive cohort without lipid-lowering treatment (n = 1802).

Methods And Results: New-onset hypertension was defined as any self-reported history of hypertension, systolic blood pressure ≥140 mmHg, or diastolic blood pressure ≥90 mmHg, or receiving antihypertensive medications at follow-up. Logistic regression models were used to evaluate the associations. Participants were aged 53.97 ± 7.49 years, 31.19% were men, and 64.54% with dyslipidemia. During a median of 2.30 years follow-up, the incidence of new-onset hypertension was 12.99%. Multivariate adjusted risks of new-onset hypertension increased with triglyceride increases (odds ratio [OR] = 1.14, 95% confidence interval [CI]: 1.03-1.27) and high-density lipoprotein cholesterol (HDL-C) decreases (OR = 0.47, 95% CI: 0.29-0.76) for one unit. However, threshold effects were observed for total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and non-HDL-C. Compared with subjects with hyperlipidemia, in those with normal concentrations of TC, LDL-C, and non-HDL-C increased risks of new-onset hypertension were observed with OR (95% CI) of 1.65 (1.10-2.46), 1.58 (1.07-2.33), and 1.57 (1.15-2.15) for one unit increasement, respectively, after adjusting for all covariates.

Conclusion: Higher TG and lower HDL-C increased the risk of new-onset hypertension, but for TC, LDL-C and non-HDLC, the risk of new-onset hypertension was increased only at normal concentrations in a Chinese community-based cohort.
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http://dx.doi.org/10.1016/j.numecd.2020.11.026DOI Listing
March 2021

Association between plasma copper levels and first stroke: a community-based nested case-control study.

Nutr Neurosci 2021 Feb 3:1-10. Epub 2021 Feb 3.

Department of Cardiovascular Medicine, the Second Affiliated Hospital of Nanchang University, Nanchang of Jiangxi, People's Republic of China.

Background And Aims: Uncertainty remains regarding the association between the risk of stroke and plasma copper levels in population with copper mostly in normal range due to limited data. We examined the association between baseline plasma copper and risk of first stroke in Chinese community-dwelling population.

Methods: We conducted a nested case control study from 'H-type Hypertension and Stroke Prevention and Control Project'. A total of 1255 first stroke cases and 1255 controls matched for age, sex and study site were included in the analysis. Conditional logistic regression analyses were performed to evaluate the association between plasma copper and first stroke.

Results: The overall mean of copper was 15.90 (2.66) μmol/L. In total, 94.26% participants' copper concentration was in the normal range by Mayo Clinic laboratory reference values. Smoothing curve showed that the associations of plasma copper with first stroke and its subtypes were linear. Each standard deviation (SD) increment of plasma copper was independently and positively associated with risk of first stroke [odds ratio (OR): 1.17, 95% confidence interval (CI): 1.07-1.28]. The multivariable ORs with 95% CIs for total stroke, ischemic stroke and hemorrhagic stroke in the highest versus the lowest quartile of plasma copper were 1.49 (1.16-1.90; -trend = 0.001), 1.46 (1.12-1.92; -trend = 0.004) and 2.05 (0.95-4.38; -trend = 0.050), respectively.

Conclusions: Baseline plasma copper was positively associated with risk of first ischemic stroke in an approximately linear fashion among Chinese community population (80.32% hypertensives), although their copper levels were mostly within the normal range according to current reference values. Our findings warrant additional investigation.
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http://dx.doi.org/10.1080/1028415X.2021.1875299DOI Listing
February 2021

Further data on the levels of emerging Fusarium mycotoxins in cereals collected from Tianjin, China.

Food Addit Contam Part B Surveill 2021 Mar 31;14(1):74-80. Epub 2021 Jan 31.

NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment , Beijing, PR China.

A number of 344 samples were collected from Tianjin, China and were analysed for the occurrence of emerging mycotoxins including enniatin A (ENN A), enniatin A (ENN A), enniatin B (ENN B), enniatin B (ENN B) and beauvericin (BEA) by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The frequencies of mycotoxins studied were 69.0% (40/58), 69.8% (37/53), 85.9% (67/78), 78.9% (75/95), and 30.0% (18/60) for rice, wheat, corn, wheat flour, and corn flour, respectively. BEA was the predominant toxin in rice (average = 37.2 μg/kg) and wheat (average = 58.4 μg/kg), followed by ENN B and ENN B, while less ENN A and ENN A were detected. ENN A was most common in corns with an average level of 28.1 μg/kg, while BEA had a higher average of 62.8 μg/kg. The levels of ENNs and BEA significantly decreased in wheat flours and corn flours, presumably due to the production process. The co-occurrences of ENNs and BEA in cereal samples were common in the combination of two and three mycotoxins and the significant positive correlations in concentrations were also obtained among them. Besides, agroclimate was considered as an important factor for production and the mycotoxin contamination was found more serious in Jizhou district, which had more rainfall and less sunshine, than the other agriculture regions. These results suggested the necessity of carrying out in-depth and large-scale monitoring of mycotoxins in cereals and their products nationwide.
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http://dx.doi.org/10.1080/19393210.2021.1873425DOI Listing
March 2021

Predictive Model for Concentration Distribution of Explosive Dispersal.

ACS Omega 2021 Jan 14;6(3):2085-2099. Epub 2021 Jan 14.

State Key Laboratory of Explosion Science and Technology, Beijing Institute of Technology, Haidian District, Beijing 100081, China.

At present, concentration of explosive dispersal is very difficult and uncertain to measure. Numerical experimentation can avoid this deficiency. Data of particles during dispersal are readily available, including velocity, displacement, and mass. However, there is minimal research on the concentration of explosive dispersal. Existing models used for the calculation of particle concentration neglect measuring the initial condition of particles and cannot, therefore, accurately describe the whole particle dispersion process. Moreover, existing concentration models do not take into account the continuous decrease in the size of particles caused by stripping and evaporation effects during flight, resulting in inaccurate descriptions of the concentration distribution. Consequently, this work derives a model to predict the concentration distribution of liquid and granular material dispersal, considering the two questions above. Concentration can be calculated based on the condensed-phase distribution and gas-phase distribution of the fuel cloud at different times by the model. This model was validated using experimental data on the mean concentration of dispersal and was well fitted. Therefore, it can be used as a tool to predict the dispersal of liquid and granular material, an explosion suppressant in coal mine accidents, and an aerosol fire extinguishant in remote forest fire extinguishers. Moreover, being able to predict the concentration of large-scale dispersal can significantly improve the accuracy and efficiency of secondary detonation.
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http://dx.doi.org/10.1021/acsomega.0c05128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841944PMC
January 2021

Detection of ascorbic acid based on its quenching effect on luminol-artemisinin chemiluminescence.

Analyst 2021 Mar 27;146(6):1981-1985. Epub 2021 Jan 27.

Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, P. R. China.

We find that luminol can react with artemisinin (ART) to produce chemiluminescence (CL) in the absence of a catalyst and ascorbic acid (AA) can quench luminol-ART CL. Based on its efficient inhibition effect on luminol-ART CL, a new AA detection method is established. The calibration curve for the determination of AA is in the linear range of 5 × 10 M to 1 × 10 M with a detection limit of 50 nM, which is more sensitive than many other reported methods. This CL approach was utilized to detect AA in vitamin C tablets by applying the standard addition method, and the recoveries of 104.0%, 96.8% and 103.4% were obtained, respectively, at concentrations of 1 μM, 5 μM and 10 μM with a RSD value of less than 3.6%. This developed method for AA assay is distinguished by its fastness, reproducibility, easy operation and good selectivity.
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http://dx.doi.org/10.1039/d0an02280bDOI Listing
March 2021

The effect of ABCA1 gene DNA methylation on blood pressure levels in a Chinese hyperlipidemic population.

J Hum Hypertens 2021 Jan 18. Epub 2021 Jan 18.

Institute of Biomedicine, Anhui Medical University, Hefei, China.

Hypertension is an important public health challenge worldwide. Epigenetic studies are providing novel insight into the underlying mechanisms of hypertension. We investigated the effect of DNA methylation in ATP-binding cassette transporter 1 (ABCA1) gene on blood pressure levels in a Chinese hyperlipidemic population. We randomly selected 211 individuals with hyperlipidemia who had not received any lipid-lowering treatment at baseline from our previous statin pharmacogenetics study (n = 734). DNA methylation loci at the ABCA1 gene were measured by MethylTarget, a next generation bisulfite sequencing-based multiple targeted cytosine-guanine dinucleotide methylation analysis method. Mean DNA methylation level was used in statistical analysis. In all subjects, higher mean ABCA1_B methylation was positively associated with systolic blood pressure (SBP) (β = 8.27, P = 0.008; β = 8.78, P = 0.005) and explained 2.7% and 5.8% of SBP variation before and after adjustment for lipids, respectively. We further divided all patients into three groups based on the tertile of body mass index (BMI) distribution. In the middle tertile of BMI, there was a significantly positive relationship between mean ABCA1_A methylation and SBP (β = 0.89, P = 0.003) and DBP (β = 0.32, P = 0.030). Mean ABCA1_A methylation explained 11.0% of SBP variation and 5.3% of DBP variation, respectively. Furthermore, mean ABCA1_A methylation (β = 0.79; P = 0.007) together with age and gender explained up to 24.1% of SBP variation. Our study provides new evidence that the ABCA1 DNA methylation profile is associated with blood pressure levels, which highlights that DNA methylation might be a significant molecular mechanism involved in the pathophysiological process of hypertension.
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http://dx.doi.org/10.1038/s41371-020-00479-zDOI Listing
January 2021

Effectiveness of chest pain centre accreditation on the management of acute coronary syndrome: a retrospective study using a national database.

BMJ Qual Saf 2020 Dec 22. Epub 2020 Dec 22.

Department of Cardiology, Peking University First Hospital, Beijing, China

Background: Large-scale real-world data to evaluate the impact of chest pain centre (CPC) accreditation on acute coronary syndrome (ACS) emergency care in heavy-burden developing countries like China are rare.

Methods: This study is a retrospective study based on data from the Hospital Quality Monitoring System (HQMS) database. This study included emergency patients admitted with ACS to hospitals that uploaded clinical data continuously to the database from 2013 to 2016. Propensity score matching was used to compare hospitals with and without CPC accreditation during this period. A longitudinal self-contrast comparison design with mixed-effects models was used to compare management of ACS before and after accreditation.

Results: A total of 798 008 patients with ACS from 746 hospitals were included in the analysis. After matching admission date, hospital levels and types and adjusting for possible covariates, patients with ACS admitted to accredited CPCs had lower in-hospital mortality (OR=0.70, 95% CI 0.53 to 0.93), shorter length of stay (LOS; adjusted multiplicative effect=0.89, 95% CI 0.84 to 0.94) and more percutaneous coronary intervention (PCI) procedures (OR=3.53, 95% CI 2.20 to 5.66) than patients admitted in hospitals without applying for CPC accreditation. Furthermore, when compared with the 'before accreditation' group only in accredited CPCs, the in-hospital mortality and LOS decreased and the usage of PCI were increased in both 'accreditation' (for in-hospital mortality: OR=0.86, 95% CI 0.79 to 0.93; for LOS: 0.94, 95% CI 0.93 to 0.95; for PCI: OR=1.22, 95% CI 1.18 to 1.26) and 'after accreditation' groups (for in-hospital mortality: OR=0.90, 95% CI 0.84 to 0.97; for LOS: 0.89, 95% CI 0.89 to 0.90; for PCI: OR=1.36, 95% CI 1.33 to 1.39). The significant benefits of decreased in-hospital mortality, reduced LOS and increased PCI usage were also observed for patients with acute myocardial infarction.

Conclusions: CPC accreditation is associated with better management and in-hospital clinical outcomes of patients with ACS. CPC establishment and accreditation should be promoted and implemented in countries with high levels of ACS.
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http://dx.doi.org/10.1136/bmjqs-2020-011491DOI Listing
December 2020

Association Between Average Plasma Potassium Levels and 30-day Mortality During Hospitalization in Patients with COVID-19 in Wuhan, China.

Int J Med Sci 2021 1;18(3):736-743. Epub 2021 Jan 1.

Department of Cardiology, Peking University First Hospital, Beijing, China.

Coronavirus disease 2019 (COVID-19) has resulted in more than 610,000 deaths worldwide since December 2019. Given the rapid deterioration of patients' condition before death, markers with efficient prognostic values are urgently required. During the treatment process, notable changes in plasma potassium levels have been observed among severely ill patients. We aimed to evaluate the association between average plasma potassium (K) levels during hospitalization and 30-day mortality in patients with COVID-19. Consecutive patients with COVID-19 hospitalized in the Zhongfaxincheng branch of Tongji Hospital in Wuhan, China from February 8 to 28, 2020 were enrolled in this study. We followed patients up to 30 days after admission. A total of 136 patients were included in the study. The average age was 62.1±14.6 years and 51.5% of patients were male. The median baseline potassium level was 4.3 (3.9-4.6) mmol/L and K level during hospitalization was 4.4 (4.2-4.7) mmol/L; the median number of times that we measured potassium was 4 (3-5). The 30-day mortality was 19.1%. A J-shaped association was observed between K and 30-day mortality. Multivariate Cox regression showed that compared with the reference group (K 4.0 to <4.5 mmol/L), 30-day mortality was 1.99 (95% confidence interval [CI]=0.54-7.35, P=0.300), 1.14 (95% CI=0.39-3.32, P=0.810), and 4.14 (95% CI=1.29-13.29, P=0.017) times higher in patients with COVID-19 who had K <4.0, 4.5 to <5.0, and ≥5.0 mmol/L, respectively. Patients with COVID-19 who had a K level ≥5.0 mmol/L had a significantly increased 30-day mortality compared with those who had a K level 4.0 to <4.5 mmol/L. Plasma potassium levels should be monitored routinely and maintained within appropriate ranges in patients with COVID-19.
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http://dx.doi.org/10.7150/ijms.50965DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797539PMC
January 2021

Expression of miRNA-203 and its target gene in hair follicle cycle development of Cashmere goat.

Cell Cycle 2021 Jan 11;20(2):204-210. Epub 2021 Jan 11.

College of Veterinary Medicine, Jilin University , Changchun, China.

MicroRNA plays an important regulatory role in the development of all organisms, including hair follicle development. In order to improve domestic cashmere yield, the role of miRNA in hair follicle cycle has become a research hotspot. However, the molecular and cellular mechanisms by which miRNA-203 regulates hair follicle development are not completely understood. In this study, we found that the relevant target genes of miRNA-203 (DDOST and NAE1) were less expressed in telogen by qPCR and Immunoblotting analysis, contrary to the expression mode of miRNA-203. The Dual-Luciferase reporter assay was used to verify the correlation between miRNA-203 and its target gene expression. The results showed that miRNA-203 specifically binds to the 3 'UTR of DDOST and NAE1, and the expression of miRNA-203 significantly down-regulates the expression of DDOST and NAE1 mRNA and protein. Therefore, this study demonstrates that miRNA-203 may regulate hair follicle development in Cashmere goats by targeting DDOST and NAE1.
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http://dx.doi.org/10.1080/15384101.2020.1867789DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889112PMC
January 2021

Novel chloramphenicol sensor based on aggregation-induced electrochemiluminescence and nanozyme amplification.

Biosens Bioelectron 2021 Mar 2;176:112944. Epub 2021 Jan 2.

Analysis and Test Center of Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China; Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables, Haikou, 571101, China. Electronic address:

Combining electrochemiluminescence (ECL) with nanozyme amplification provides unique advantages for the detection of antibiotic residues. Herein, a molecularly imprinted chloramphenicol (CAP) sensor was established based on aggregation-induced (AI)-ECL and nanozyme amplification. Covalent organic framework materials with AI-ECL groups (COF-AI-ECL) and nanozyme CoO were synthesised as the signal element and the amplification element, respectively. Subsequently, using CAP as a template molecule, a molecularly imprinted polymer (MIP) was fabricated on the electrode surface modified with COF-AI-ECL and CoO. The ECL signal of COF-AI-ECL was catalytically amplified by CoO, whereas CAP effectively quenched this signal. Consequently, the ECL signal was controlled by the elution and adsorption of CAP by the MIP, thus establishing a new method for CAP detection. Unlike traditional ECL reagent, COF-AI-ECL exhibited a stable and strong ECL signal. Therefore, COF-AI-ECL in combination with the MIP provided greater sensitivity and enhanced selectivity. The linear range of the developed CAP sensor was 5 × 10 to 4 × 10 mol/L, with a detection limit of 1.18 × 10 mol/L. Moreover, the recoveries range of 85.0%-106.2% were obtained for the detection of CAP in real honey, milk, and chicken samples, indicating the potential of this sensor design for the detection of trace antibiotic residues in food safety applications.
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http://dx.doi.org/10.1016/j.bios.2020.112944DOI Listing
March 2021

Prognostic implication of glycolysis related gene signature in non-small cell lung cancer.

J Cancer 2021 1;12(3):885-898. Epub 2021 Jan 1.

Department of Pulmonary and Critical Care Medicine, Qilu Hospital of Shandong University, Jinan 250012, China.

Abnormal glycolysis is one of the hallmarks of cancer and plays an important role in its development. This study was devoted to identify glycolysis related genes as prognostic biomarkers for non-small cell lung cancer (NSCLC). The mRNA expression profile and clinical follow-up data were obtained using The Cancer Genome Atlas (TCGA) database. The validation set was obtained by bootstrap method of random repeated sampling. A total of 200 glycolysis-related genes were obtained from Gene Set Enrichment Analysis (GSEA) and 46 genes were significantly associated with overall survival (OS). Five genes (PKP2, LDHA, HMMR, COL5A1 and B3GNT3) were eventually identified to calculate risk score of NSCLC patients. The univariate and multivariate Cox regression analysis indicated that the risk score was an independent prognostic factor (training set: HR=2.126, 95% CI [1.605, 2.815], <0.001; validation set: HR=2.298, 95%CI [1.450, 3.640], <0.001). Patients assigned to the high-risk group were associated with poor OS compared with patients in the low-risk group (training set: =7.946e-06; validation set: =6.368e-07). Receiver operating characteristic (ROC) curve and stratification analysis also demonstrated the potential prognostic performance. In conclusion, we constructed a novel glycolysis related risk signature which might contribute to predicting the prognosis of NSCLC.
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http://dx.doi.org/10.7150/jca.50274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778529PMC
January 2021

Coronary Angiography-Derived Index of Microvascular Resistance.

Front Physiol 2020 16;11:605356. Epub 2020 Dec 16.

Department of Cardiology, Peking University First Hospital, Beijing, China.

A coronary angiography-derived index of microvascular resistance (caIMR) is proposed for physiological assessment of microvasular diseases in coronary circulation. The aim of the study is to assess diagnostic performance of caIMR, using wire-derived index of microvascular resistance (IMR) as the reference standard. IMR was demonstrated in 56 patients (57 vessels) with stable/unstable angina pectoris and no obstructive coronary arteries in three centers using the Certus pressure wire. Based on the aortic pressure wave and coronary angiograms from two projections, the caIMR was computed and assessed in blinded fashion against the IMR at an independent core laboratory. Diagnostic accuracy, sensitivity, specificity, positive predictive value and negative predictive value of the caIMR with a cutoff value of 25 were 84.2% (95% CI: 72.1% to 92.5%), 86.1% (95% CI: 70.5% to 95.3%), 81.0% (95% CI: 58.1% to 94.6%), 88.6% (95% CI: 76.1% to 95.0%), and 77.3% (95% CI: 59.5% to 88.7%) against the IMR with a cutoff value of 25. The receiver-operating curve had area under the curve of 0.919 and the correlation coefficient equaled to 0.746 between caIMR and wire-derived IMR. Hence, caIMR could eliminate the need of a pressure wire, reduce technical error, and potentially increase adoption of physiological assessment of microvascular diseases in patients with ischemic heart disease.
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http://dx.doi.org/10.3389/fphys.2020.605356DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772433PMC
December 2020