Publications by authors named "Jianmei Wang"

148 Publications

Joint Transcriptomic and Metabolic Analysis of Flavonoids in Leaves.

ACS Omega 2021 Apr 28;6(13):9028-9038. Epub 2021 Mar 28.

Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou University, Taizhou 318000, China.

Flavonoids are a class of commonly occurring natural compounds in the plant kingdom with various biological activities. This study compares the content of flavonoids in at different developmental stages to better inform the selection of the optimal picking period. Thus, we analyzed the transcriptome and metabolome of at different developmental stages. The transcriptome analysis revealed 44 genes involved in the biosynthesis of flavonoids in , with 10 differentially expressed genes across the four different developmental stages. The metabolites were separated and identified by a combination of chromatography and mass spectrometry, followed by multi-reaction monitoring mode analysis of triple quadrupole mass spectrometry for complete metabolite quantification. In the flavonoid synthesis pathway, a total of 137 differential flavonoids were detected. The joint transcriptome and metabolome analysis showed that the expression trends in differential metabolites and genes were significantly related. Four MYB transcription factors and two bHLH transcription factors that are closely related to flavonoid biosynthesis were identified. The regulation network of flavonoid biosynthesis in was thus established, providing guidance for follow-up research.
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http://dx.doi.org/10.1021/acsomega.1c00059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8028134PMC
April 2021

Fungicide bromuconazole has the potential to induce hepatotoxicity at the physiological, metabolomic and transcriptomic levels in rats.

Environ Pollut 2021 Mar 12;280:116940. Epub 2021 Mar 12.

Zhejiang Province Key Laboratory for Food Safety, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, China. Electronic address:

Bromuconazole (BROMU), a representative triazole fungicide, has been widely used in agriculture for its low cost and highly efficiency against various fungi. BROMU residue was often detected in the environment and food chain, even though there is indication of health risk to animals, and in humans. However, the data related to the toxicity of BROMU in animals remains unclear, and the mechanism is still not fully elucidated. Here, male adult rats were exposed to 0, 13.8, 32.8 and 65.6 mg/kg/d of BROMU for 10 days by oral gavage. It was observed that short time BROMU exposure not only caused liver histological damage, including vacuolar degeneration of hepatocytes with pyknotic nuclei, but also changed the levels of some hepatic physiological parameters, including aspartate transaminase (AST), triglyceride (TG), pyruvate and total cholesterol (TC), indicating that BROMU causes hepatotoxicity in rats. In addition, according to the transcriptomics and metabolomics analysis, a total of 58 metabolites and 259 genes significantly changed in the high-dose BROMU treated group. Although several different pathways are involved, lipid metabolism- and bile acids metabolism-related pathways were highlighted in both metabolomics and transcriptomics analysis. More importantly, further validation had proven that BROMU could not only interact with peroxisome proliferator-activated receptor γ (PPAR-γ), but also significantly decrease its protein and gene expression in the liver, supporting that BROMU decreased the TG synthesis via inhibiting the PPAR-γ pathway. These results clearly showed that BROMU exposure could result in hepatotoxicity at metabolomic and transcriptomic level in rats. These observations could provide some important steps toward understanding the mechanism underlying BROMU-induced mammalian toxicity.
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http://dx.doi.org/10.1016/j.envpol.2021.116940DOI Listing
March 2021

The SARS-CoV-2 receptor-binding domain preferentially recognizes blood group A.

Blood Adv 2021 03;5(5):1305-1309

Joint Program in Transfusion Medicine, Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA.

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http://dx.doi.org/10.1182/bloodadvances.2020003259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7929867PMC
March 2021

Graphene-based materials for adsorptive removal of pollutants from water and underlying interaction mechanism.

Adv Colloid Interface Sci 2021 Mar 20;289:102360. Epub 2021 Jan 20.

Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB T6G 1H9, Canada. Electronic address:

Graphene-based materials have received much attention as attractive candidates for the adsorptive removal of pollutants from water due to their large surface area and diverse active sites for adsorption. The design of graphene-based adsorbents for target pollutants is based on the underlying adsorption mechanisms. Understanding the adsorption performance of graphene-based materials and its correlation to the interaction mechanisms between the pollutants and adsorbents is crucial to the further development of graphene-based functional materials and their practical applications. This review summarizes recent advances on the development of graphene-based materials for the adsorption of heavy metal ions, dyes, and oils, and the co-adsorption of their mixture from water. The material design, performance, regeneration and reuse of adsorbents, and the associated adsorption mechanisms are discussed. Various techniques for mechanistic studies of the adsorption of heavy metal ions, dyes, and oils on graphene-based materials are highlighted. The remaining challenges and perspectives for future development and investigation of graphene-based materials as adsorbents are also presented.
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http://dx.doi.org/10.1016/j.cis.2021.102360DOI Listing
March 2021

Comparison of Antibody Class-Specific SARS-CoV-2 Serologies for the Diagnosis of Acute COVID-19.

J Clin Microbiol 2021 03 19;59(4). Epub 2021 Mar 19.

Center for Transfusion Medicine and Cellular Therapies, Emory University School of Medicine, Atlanta, Georgia, USA

Accurate diagnosis of acute severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is critical for appropriate management of patients with this disease. We examined the possible complementary role of laboratory-developed class-specific clinical serology in assessing SARS-CoV-2 infection in hospitalized patients. Serological tests for immunoglobulin G (IgG), IgA, and IgM antibodies against the receptor binding domain (RBD) of SARS-CoV-2 were evaluated using samples from real-time reverse transcription-quantitative PCR (qRT-PCR)-confirmed inpatient coronavirus disease 2019 (COVID-19) cases. We analyzed the influence of timing and clinical severity on the diagnostic value of class-specific COVID-19 serology testing. Cross-sectional analysis revealed higher sensitivity and specificity at lower optical density cutoffs for IgA in hospitalized patients than for IgG and IgM serology (IgG area under the curve [AUC] of 0.91 [95% confidence interval {CI}, 0.89 to 0.93] versus IgA AUC of 0.97 [95% CI, 0.96 to 0.98] versus IgM AUC of 0.95 [95% CI, 0.92 to 0.97]). The enhanced performance of IgA serology was apparent in the first 2 weeks after symptom onset and the first week after PCR testing. In patients requiring intubation, all three tests exhibit enhanced sensitivity. Among PCR-negative patients under investigation for SARS-CoV-2 infection, 2 out of 61 showed clear evidence of seroconversion IgG, IgA, and IgM. Suspected false-positive results in the latter population were most frequently observed in IgG and IgM serology tests. Our findings suggest the potential utility of IgA serology in the acute setting and explore the benefits and limitations of class-specific serology as a complementary diagnostic tool to PCR for COVID-19 in the acute setting.
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http://dx.doi.org/10.1128/JCM.02026-20DOI Listing
March 2021

The spatial variation of soil bacterial community assembly processes affects the accuracy of source tracking in ten major Chinese cities.

Sci China Life Sci 2021 Jan 8. Epub 2021 Jan 8.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.

Urban soils harbor billions of bacterial cells and millions of species. However, the distribution patterns and assembly processes of bacterial communities remain largely uncharacterized in urban soils. It is also unknown if we can use the bacteria to track soil sources to certain cities and districts. Here, Illumina MiSeq sequencing was used to survey soil bacterial communities from 529 random plots spanning 61 districts and 10 major cities in China. Over a 3,000 km range, community similarity declined with increasing geographic distance (Mantel r=0.62), and community composition was clustered by city (R=0.50). Within cities (<100 km), the aforementioned biogeographic patterns were weakened. Process analysis showed that homogenizing dispersal and dispersal limitation dominated soil bacterial assembly at small and large spatial scales, respectively. Accordingly, the probabilities of accurately tracking random soil sources to certain cities and districts were 90.0% and 66.7%, respectively. When the tested samples originated from cities that were more than 1,265 km apart, the soil sources could be identified with nearly 100% accuracy. Overall, this study demonstrates the strong distance-decay relationship and the clear geographic zoning of urban soil bacterial communities among cities. The varied importance of different community assembly processes at multiple spatial scales strongly affects the accuracy of microbial source tracking.
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http://dx.doi.org/10.1007/s11427-020-1843-6DOI Listing
January 2021

High-throughput method based on a novel thin-film microextraction coating for determining macrolides and lincosamides in honey.

Food Chem 2021 Jun 30;346:128920. Epub 2020 Dec 30.

State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, PR China. Electronic address:

A high-throughput method using a new ZIF-8@GO thin-film microextraction coating was established for determining macrolides and lincosamides in honey. The coating preparation parameters (ZIF-8@GO synthesis conditions, coating material proportions, dipping time) and analysis parameters (sample diluent solvent, adsorption and desorption conditions using the ZIF-8@GO coating) were optimized. The optimized parameters were: diluent solvent sodium carbonate/sodium bicarbonate buffer solution (pH 9), adsorption time 45 min, desorption time 5 min, desorption solvent 45:40:15 v/v/v methanol/acetonitrile/water. The extracted targets were determined by ultra-high performance liquid chromatography tandem mass spectrometry. The recoveries of 10 analytes were 67.5-107.2% and the detection and quantification limits were 0.1-0.4 and 0.4-1.4 μg/kg, respectively. The method could analyze 96 samples per run. The minimal manual time and effort is required since the bulk of the sample processing is fully automated. It was a useful and efficient method for monitoring drug residues and was successfully used to analyze real samples.
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http://dx.doi.org/10.1016/j.foodchem.2020.128920DOI Listing
June 2021

MNSFβ Promotes the Proliferation and Migration of Human Extravillous Trophoblast Cells and the Villus Expression Level of MNSFβ Is Decreased in Recurrent Miscarriage Patients.

Gynecol Obstet Invest 2020 Dec 16:1-13. Epub 2020 Dec 16.

NHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Pharmacy School, Fudan University, Shanghai, China.

Aims: The invasion of extravillous trophoblast (EVT) cells into maternal decidua is essential for the establishment and maintenance of pregnancy. Derangement of EVT cell invasion might cause pregnancy complications including recurrent miscarriage (RM). We previously reported that deficiency of monoclonal nonspecific suppressor factor beta (MNSFβ) led to the early pregnancy failure in mice and the decidual MNSFβ expression level in RM patients was significantly decreased, but the underlying molecular mechanism of the role that MNSFβ played at the maternal-fetal interface remains unclear. Thus, in the present study, we determined effects of downregulated MNSFβ expression on human EVT cell activities.

Methods: The MNSFβ expression in first-trimester human decidual and placental villus tissues was detected, respectively, by immunofluorescence or immunohistochemical analyses. The MNSFβ expression level in the immortalized first-trimester human EVT cell line HTR8/SVneo was downregulated by transfecting the small interfering RNA against MNSFβ and upregulated by transfecting the recombinant pDsRed-MNSFβ plasmids. The proliferation, migration, invasion, and apoptosis activities of HTR8/SVneo cells were, respectively, determined by cytometry assay, scratch test, transwell assay, and FITC/PI staining. The expression levels of P53, RhoA, Bcl-2, Bax, and MMP-9 in HTR8/SVneo cells, as well as the expression levels of MNSFβ and RhoA in placental villi of RM patients and physically normal pregnant women (NP), were examined by Western blot analysis.

Results: MNSFβ protein signals were observed in first-trimester human villus and extravillous trophoblast cells. The downregulated MNSFβ expression significantly attenuated the proliferation, migration, and invasion abilities of HTR8/SVneo cells, accompanied with the obviously decreased expression levels of P53, RhoA, Bcl-2, Bax, and MMP-9, whereas the upregulated MNSFβ expression in HTR8/SVneo cells represented the inverse effects. Furthermore, expression levels of MNSFβ and RhoA in first-trimester human placental villus tissues of RM patients were significantly decreased compared to that of NP women.

Conclusion: These data suggested that MNSFβ promotes proliferation and migration of human EVT cells, probably via the P53 signaling pathway, and the deficiency of MNSFβ in placental villi might lead to early pregnancy loss by reducing proliferation and invasion activities of EVTs.
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http://dx.doi.org/10.1159/000506309DOI Listing
December 2020

Quantitative measurements of IR780 in formulations and tissues.

J Pharm Biomed Anal 2021 Feb 20;194:113780. Epub 2020 Nov 20.

Department of Pharmaceutical Sciences, University of North Texas Health Science Center, Fort Worth, TX, USA. Electronic address:

Purpose: IR780 iodide, a promising near-infrared dye, is widely used to prepare nanoparticles as a theranostic agent for tumor imaging and therapy. However, there are no validated (bio)analytical methods to measure IR780 in nanoparticles and tissues in literature. The aim of this study is to develop and validate a new HPLC method to measure IR780 concentration in IR780 formulations as well as a new LC-MS/MS method to measure IR780 concentration in tissue samples, particularly in liver and lung.

Materials And Methods: IR780 granules that produced IR780 in situ self-assembled nanoparticles upon contact with water were prepared at two drug loadings (0.2 % and 0.37 %). An HPLC method was developed and validated to measure IR780 concentrations in IR780 granules and nanoparticles. Furthermore, a validated LC-MS/MS method was developed to measure IR780 in mouse liver and lung. Both HPLC method and LC-MS/MS method were validated in terms of specificity, stability, linearity, limit of detection, limit of quantification, accuracy and precision.

Results: Both HPLC method and LC-MS/MS method achieved the criteria for method validation. The HPLC method was accurate in the concentration range of 0.5-25 μg/mL. The measured drug loadings were 95 % of the theoretical drug loadings. The validated LC-MS/MS method can quantitatively measure the concentrations of IR780 in liver and lung. The linear range of the LC-MS/MS method was 1-1000 ng/mL for both liver and lung samples. IR780 granules showed the lung selectivity compared to IR780 solution at 2 h after oral administration.

Conclusion: A validated HPLC method was developed to measure IR780 concentration in pharmaceutical formulations and a validated LC-MS/MS method was developed to measure IR780 concentration in tissues. These quantitative methods provide reliable measurements of IR780 in pharmaceutic formulations and biological samples, which will significantly facilitate the research of IR780 as a theranostic agent for cancer therapy and imaging.
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http://dx.doi.org/10.1016/j.jpba.2020.113780DOI Listing
February 2021

Evaluation of cyromazine transferred from feed to chicken products and subsequent assessment of dietary risks to Chinese consumers.

J Food Sci 2020 Dec 20;85(12):4396-4406. Epub 2020 Nov 20.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, National Reference Laboratory for Agricultural Testing (Pesticide Residues), Agricultural Ministry Key Laboratory for Pesticide Residue Detection, Zhejiang Province Key Laboratory for Food Safety, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021, P. R. China.

In this work, levels of cyromazine and its metabolite melamine in chicken products and its transfer factors (TFs) and dietary risks assessment for Chinese consumers are presented. Cyromazine was added to chicken feed at doses of 5, 25, and 50 mg/kg for 42 days. Cyromazine residues were found in frequently consumed chicken products (meat, heart, liver, gizzard, and eggs), with liver and eggs displaying the highest concentrations (0.935 and 1.281 mg/kg, respectively). Low levels of melamine residues (<0.029 mg/kg) were detected in chicken products from chickens treated with 25 and 50 mg/kg cyromazine, but not in eggs from chickens treated with 5 mg/kg cyromazine. The TFs for the investigated chicken products varied from 0.0074 to 0.0229 across the dosage levels. The chronic exposure assessment showed that 0.001 to 0.190% of the acceptable daily intake (0.06 mg/kg body weight [b.w.]/day) of cyromazine was consumed through chicken products among the various age and gender groups of Chinese consumers. The acute exposure assessments of different age and gender groups were 0.0004% to 0.178% of the acute reference dose (0.1 mg/kg b.w.). Although the results suggest that the risk associated with cyromazine residues in chicken products was low in China, the possible risk associated with cyromazine residues in chicken products should not be ignored while cyromazine remains a legal feed additive. PRACTICAL APPLICATION: The present analytical method could be used for cyromazine and melamine detection in different chicken products, and dietary risk assessments of cyromazine provided a support for the work of regulatory bodies to conduct surveillance programs regarding food safety evaluation of cyromazine.
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http://dx.doi.org/10.1111/1750-3841.15512DOI Listing
December 2020

Abscisic acid receptors maintain abscisic acid homeostasis by modulating UGT71C5 glycosylation activity.

J Integr Plant Biol 2021 Mar;63(3):543-552

Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, China.

Uridine diphosphate-glucosyltransferases (UGTs) maintain abscisic acid (ABA) homeostasis in Arabidopsis thaliana by converting ABA to abscisic acid-glucose ester (ABA-GE). UGT71C5 plays an important role in the generation of ABA-GE. Abscisic acid receptors are crucial upstream components of the ABA signaling pathway, but how UGTs and ABA receptors function together to modulate ABA levels is unknown. Here, we demonstrated that the ABA receptors RCAR12/13 and UGT71C5 maintain ABA homeostasis in Arabidopsis following rehydration under drought stress. Biochemical analyses show that UGT71C5 directly interacted with RCAR8/12/13 in yeast cells, and the interactions between UGT71C5 and RCAR12/13 were enhanced by ABA treatment. Enzyme activity analysis showed that ABA-GE contents were significantly elevated in the presence of RCAR12 or RCAR13, suggesting that these ABA receptors enhance the activity of UGT71C5. Determination of the content of ABA and ABA-GE in Arabidopsis following rehydration under drought stress revealed that ABA-GE contents were significantly higher in Arabidopsis plants overexpressing RCAR12 and RCAR13 than in non-transformed plants and plants overexpressing RCAR11 following rehydration under drought stress. These observations suggest that RCAR12 and RCAR13 enhance the activity of UGT71C5 to glycosylate excess ABA into ABA-GE following rehydration under drought stress, representing a rapid mechanism for regulating plant growth and development.
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http://dx.doi.org/10.1111/jipb.13030DOI Listing
March 2021

Characterization of abscisic acid (ABA) receptors and analysis of genes that regulate rutin biosynthesis in response to ABA in Fagopyrum tataricum.

Plant Physiol Biochem 2020 Dec 10;157:432-440. Epub 2020 Nov 10.

Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China. Electronic address:

Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) is a nutritional crop, which has high rutin, and is good for health. Until now, plant genetic engineering is insufficient for Tartary buckwheat. Abscisic acid (ABA), as one of phytohormones, is involved in the regulation of plant growth and development, and responses to diverse environmental challenges. Although ABA receptors have been well characterized in Arabidopsis, it is little understood in Tartary buckwheat. In this study, we identified 12 ABA receptors, designated as FtRCAR1 through FtRCAR12 in Tartary buckwheat. FtRCARs are divided into three subfamily. Based on the similarity, we could predict that FtRCARs comprise of the monomeric (FtRCAR1, 3, 4, 5, 9, 10, 11 and 12) and the dimeric (FtRCAR2, 7 and 8) state in solution. The analysis of the transcript pattern indicated that most of FtRCARs were significantly variable among the root, stem, leaf, flower and seed, while FtRCAR4 transcript was undetectable under in all tissues. The transcript levels of FtRCARs under ABA treatment indicated that most FtRCARs transcripts were depressed, indicating a possible feedback regulation of ABA signaling. The analysis of rutin biosynthesis related-genes indicated that ABA up-graduated CHS, CHI, F3'H, F3H and FLS transcript levels, while transcripts of 4CL and PAL were down-regulated. In addition, the transcription factors that mediated the rutin biosynthesis related-genes were also regulated by exogenous ABA. Thus, the identification and the characterization of FtRCARs would enable us to further understand the role of ABA signal in Tartary buckwheat.
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http://dx.doi.org/10.1016/j.plaphy.2020.11.005DOI Listing
December 2020

Transcriptome Reveals Multi Pigmentation Genes Affecting Dorsoventral Pattern in Avian Body.

Front Cell Dev Biol 2020 1;8:560766. Epub 2020 Oct 1.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.

Certain animals exhibit a special dorsoventral pattern with a lighter ventral side compared to the dorsal one and this phenomenon was preserved in the long-term evolution process. Birds also retain this trait. Recently, Inaba et al. (2019) found that (agouti signal protein) regulated interconversion between different melanocyte types leads to dorsal stripe pattern, which may partly explain the birds' dorsoventral plumage color difference. In this study, we used the embryo samples of LBM (light brown mottling) ducks () with white ventral and dark dorsal body parts to investigate the mechanism of dorsoventral color variation. Firstly, melanin deposition process of duck embryos was investigated. The result indicated that E13 and E16 were the active stages of melanin synthesis. Moreover, the melanin deposition on the dorsum of LBM ducks was higher than that on the ventral side throughout. Then, RNA-seq was conducted for the dorsal and ventral skin tissues from E7 (early), E13 (middle) and E19 (late) of LBM ducks. Expression pattern analysis showed that the mRNA expression of most melanin synthesis related genes were at the highest level at E13, which was consistent with the section analysis. A correlation was found between melanogenesis pathway and dorsoventral color difference by co-expression analysis. In the DEG (differentially expressed gene) analysis, we added the dorsal skin transcriptome of embryonic white and black duck of same subspecies () for horizontal comparison. The results showed that 8 melanogenesis related genes (, , , , , , , and ) were the common DEGs (Differential expressed genes) in the comparisons of body parts and breeds suggesting that the underlying molecular regulatory mechanism of dorsoventral plumage color difference may be similar to that of albino and melanic duck, which were caused by the different expression of multiple genes in melanin synthesis pathway. In addition, the molecular regulation of melanin synthesis pathway in the dorsal and ventral side of LBM ducks was analyzed. In this pathway, , , , and have differential mRNA expression. , as an upstream gene in this pathway, was likely to play a decisive role in determining the dorsoventral plumage pattern.
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http://dx.doi.org/10.3389/fcell.2020.560766DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559526PMC
October 2020

In-situ probing of electrochemical dissolution and surface properties of chalcopyrite with implications for the dissolution kinetics and passivation mechanism.

J Colloid Interface Sci 2021 Feb 6;584:103-113. Epub 2020 Oct 6.

Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta T6G 1H9, Canada. Electronic address:

Hypothesis: The anodic dissolution of chalcopyrite (CuFeS) encounters the problem of surface passivation, which significantly affects the copper extraction efficiency. So far, there is no agreement on the passivation mechanism and composition of passive layer, which could be studied by using in-situ scanning electrochemical microscopy (SECM).

Experiments: SECM was applied for the in-situ probing of chalcopyrite dissolution under mild oxidation potentials. The surface hydrophobicity and nanoscale distribution of hydrophobic domains were analyzed by static water contact angle measurement and atomic force microscope (AFM) force mapping, respectively. The surface conductivity was characterized by SECM feedback mode.

Findings: The concentrations of released species Fe, Cu and soluble copper sulfide species (CuS) generally increased with the potential of chalcopyrite. In the active region (low potentials), Fe was preferentially released, and the metal-deficient sulfide layer that was rich in copper relative to iron started to form as the passive layer. While the release of Fe and Cu was impeded in the passive region, the detected CuS became pronounced in this region and the following transpassive region, which suggested that the existence of CuS was a result of passive layer dissolution. The nanoscale distribution of hydrophobic domains suggested that the formation of hydrophobic passive layer initiated in the active region and this layer almost completely covered the chalcopyrite surface at the beginning of passive region. The surface conductivity of chalcopyrite decreased with potential due to the formation of less conductive metal-deficient sulfide layer and possibly insulating elemental sulfur (in the transpassive region). This work provides a new approach for the in-situ probing of chalcopyrite dissolution and useful insights into its dissolution kinetics and passivation mechanisms, with implications for similar electrochemical processes of other mineral surfaces.
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http://dx.doi.org/10.1016/j.jcis.2020.09.115DOI Listing
February 2021

Retraction Note to: Attenuation of aortic injury by ursolic acid through RAGE-Nox-NFκB pathway in streptozocin-induced diabetic rats.

Arch Pharm Res 2020 Sep;43(9):983

Pharmacology Department, Henan Provincial Institute of Food and Drug Control, Zhengzhou, 450003, China.

The authors have retracted this article [1] because Fig. 4A and Fig. 4B are same as the left-hand panel and the middle panel respectively in Fig. 1 in an article published in Chinese by another author group [2]. In addition, Associate Professor Xiaoning Li did not give permission to be acknowledged for pathology work. All authors agree with this retraction.
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http://dx.doi.org/10.1007/s12272-020-01262-xDOI Listing
September 2020

Ambient PM exposures and systemic biomarkers of lipid peroxidation and total antioxidant capacity in early pregnancy.

Environ Pollut 2020 Nov 4;266(Pt 2):115301. Epub 2020 Aug 4.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, China. Electronic address:

Evidence for effects of PM on systemic oxidative stress in pregnant women is limited, especially in early pregnancy. To estimate the associations between ambient PM exposures and biomarkers of lipid peroxidation and total antioxidant capacity (T-AOC) in women with normal early pregnancy (NEP) and women with clinically recognized early pregnancy loss (CREPL), 206 early pregnant women who had measurements of serum malondialdehyde (MDA) and T-AOC were recruited from a larger case-control study in Tianjin, China from December 2017 to July 2018. Ambient PM concentrations of eight single-day lags exposure time windows before blood collection at the women's residential addresses were estimated using temporally-adjusted land use regression models. Effects of PM exposures on percentage change in the biomarkers were estimated using multivariable linear regression models adjusted for month, temperature, relative humidity, gestational age and other covariates. Unconstrained distributed lag models were used to estimate net cumulative effects. Increased serum MDA and T-AOC were significantly associated with increases in PM at several lag exposure time windows in both groups. The net effects of each interquartile range increase in PM over the preceding 8 days on MDA were significantly higher (p < 0.001) in CREPL [52% (95% CI: 41%, 62%)] than NEP [22% (95% CI: 9%, 36%)] women. Net effects of each interquartile range increase in PM over the preceding 5 days on T-AOC were significantly lower (p = 0.010) in CREPL [14% (95% CI: 9%, 19%)] than NEP [24% (95% CI: 18%, 29%)] women. Exposure to ambient PM may induce systemic lipid peroxidation and antioxidant response in early pregnant women. More severe lipid peroxidation and insufficient antioxidant capacity associated with PM was found in CREPL women than NEP women. Future studies should focus on mechanisms of individual susceptibility and interventions to reduce PM-related oxidative stress in the first trimester.
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http://dx.doi.org/10.1016/j.envpol.2020.115301DOI Listing
November 2020

, an E3 Ubiquitin Ligase, Enhances the Thermotolerance in .

Plants (Basel) 2020 Aug 21;9(9). Epub 2020 Aug 21.

Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China.

E3 ubiquitin ligase plays a vital role in the ubiquitin-mediated heat-related protein degradation pathway. Herein, we report that the expression of , a C3HC4 zinc-finger ubiquitin E3 ligase gene, was induced by heat stress, and the β-glucuronidase () gene driven by the promoter has shown increased activity after basal and acquired thermotolerance. To further explore the function of in heat stress response (HSR), we used the mutant and -overexpressing lines (OE2 and OE10) to expose in heat shock. In this study, the mutant had a lower germination and survival rate than those of Col-0 when suffered from the heat stress, whereas OEs enhanced basal and acquired thermotolerance in seedlings. When compared to Col-0 and OEs, loss-of-function in resulted in lower chlorophyll retention and higher content of reactive oxygen species (ROS) after heat treatment. Moreover, the transcript levels of and several heat-related genes ( and ) were upregulated to greater extents in OEs and lower extents in compared to Col-0 after heat treated. Hence, we suggest that may act as a positive role in regulating the high temperature by mediating the degradation of unknown target proteins.
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http://dx.doi.org/10.3390/plants9091074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569766PMC
August 2020

Close arrangement of CARK3 and PMEIL affects ABA-mediated pollen sterility in Arabidopsis thaliana.

Plant Cell Environ 2020 11 9;43(11):2699-2711. Epub 2020 Sep 9.

Key Laboratory of Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, China.

Abscisic acid (ABA) signaling is a vital plant signaling pathway for plant responses to stress conditions. ABA treatment can alter global gene expression patterns and cause significant phenotypic changes. We investigated the responses to ABA treatment during flowering in Arabidopsis thaliana. Dipping the flowers of CARK3 T-DNA mutants in ABA solution, led to less reduction of pollen fertility than in the wild type plants (Col-0). We demonstrated that PMEIL, a gene located downstream of CARK3, directly affects pollen fertility. Due to the close arrangement of CARK3 and PMEIL, CARK3 expression represses transcription of PMEIL in an ABA-dependent manner through transcriptional interference. Our study uncovers a molecular mechanism underlying ABA-mediated pollen sterility and provides an example of how transcriptional interference caused by close arrangement of genes may mediate stress responses during plant reproduction.
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http://dx.doi.org/10.1111/pce.13871DOI Listing
November 2020

Cosmc controls B cell homing.

Nat Commun 2020 08 10;11(1):3990. Epub 2020 Aug 10.

Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.

The molecular mechanisms regulating lymphocyte homing into lymph nodes are only partly understood. Here, we report that B cell-specific deletion of the X-linked gene, Cosmc, and the consequent decrease of protein O-glycosylation, induces developmental blocks of mouse B cells. After transfer into wild-type recipient, Cosmc-null B cells fail to home to lymph nodes as well as non-lymphoid organs. Enzymatic desialylation of wild-type B cells blocks their migration into lymph nodes, indicating a requirement of sialylated O-glycans for proper trafficking. Mechanistically, Cosmc-deficient B cells have normal rolling and firm arrest on high endothelium venules (HEV), thereby attributing their inefficient trafficking to alterations in the subsequent transendothelial migration step. Finally, Cosmc-null B cells have defective chemokine signaling responses. Our results thus demonstrate that Cosmc and its effects on O-glycosylation are important for controlling B cell homing.
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http://dx.doi.org/10.1038/s41467-020-17765-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417590PMC
August 2020

AtPPRT3, a novel E3 ubiquitin ligase, plays a positive role in ABA signaling.

Plant Cell Rep 2020 Nov 5;39(11):1467-1478. Epub 2020 Aug 5.

Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, China.

Key Message: The RING-type E3 ligase AtPPRT3 participates in the plant ABA responding as a positive regulator. E3 ubiquitin ligase, alike of classic plant stress resistance proteins, plays a vital role in regulating the degradation of stress-related proteins. In this study, we investigated the function of the RING-type E3 ubiquitin ligase AtPPRT3 in the ABA signaling pathway. AtPPRT3, located in the endoplasmic reticulum membrane, is involved in ABA signaling. The transcriptional expression of AtPPRT3 was induced by ABA, and the promoter region upstream of AtPPRT3 contains the ABA-responsive element (ABRE). Additionally, the β-glucuronidase (GUS) gene driven by the AtPPRT3 promoter was up-regulated in transgenic plants after ABA treated. We obtained AtPPRT3 function-deficient mutants atpprt3-1, atpprt3-2, and AtPPRT3 over-expressing lines (OE4 and OE5). In this study, atpprt3-1 and atpprt3-2 were less sensitive to exogenous ABA compared to Col-0, whereas OE4 and OE5 were more sensitive. Moreover, AtPPRT3 promotes ABA-mediated stomatal closure and inhibits water loss in Arabidopsis thaliana. After exogenous ABA treated, the transcriptional expression levels of AtDREB2A, AtKIN1, AtRD29A, AtERD10 and AtRD29B were up-regulated to greater extents in OEs and lower extents in atpprt3-1 and atpprt3-2 compared to Col-0. These results suggest that AtPPRT3 positively regulates ABA signaling in A. thaliana.
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http://dx.doi.org/10.1007/s00299-020-02575-3DOI Listing
November 2020

Determination of metformin bio-distribution by LC-MS/MS in mice treated with a clinically relevant paradigm.

PLoS One 2020 11;15(6):e0234571. Epub 2020 Jun 11.

Department of Pharmacology and Neuroscience, Institute for Healthy Aging, University of North Texas Health Science Center, Fort Worth, Texas, United States of America.

Metformin, an anti-diabetes drug, has been recently emerging as a potential "anti-aging" intervention based on its reported beneficial actions against aging in preclinical studies. Nonetheless, very few metformin studies using mice have determined metformin concentrations and many effects of metformin have been observed in preclinical studies using doses/concentrations that were not relevant to therapeutic levels in human. We developed a liquid chromatography-tandem mass spectrometry protocol for metformin measurement in plasma, liver, brain, kidney, and muscle of mice. Young adult male and female C57BL/6 mice were voluntarily treated with metformin of 4 mg/ml in drinking water which translated to the maximum dose of 2.5 g/day in humans. A clinically relevant steady-state plasma metformin concentrations were achieved at 7 and 30 days after treatment in male and female mice. Metformin concentrations were slightly higher in muscle than in plasma, while, ~3 and 6-fold higher in the liver and kidney than in plasma, respectively. Low metformin concentration was found in the brain at ~20% of the plasma level. Furthermore, gender difference in steady-state metformin bio-distribution was observed. Our study established steady-state metformin levels in plasma, liver, muscle, kidney, and brain of normoglycemic mice treated with a clinically relevant dose, providing insight into future metformin preclinical studies for potential clinical translation.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0234571PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289415PMC
August 2020

E3 ubiquitin ligase ATL61 acts as a positive regulator in abscisic acid mediated drought response in Arabidopsis.

Biochem Biophys Res Commun 2020 07 1;528(2):292-298. Epub 2020 Jun 1.

Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, State Key Laboratory of Hydraulics and Mountain River Engineering, College of Life Sciences, Sichuan University, Chengdu, 610065, China. Electronic address:

Abscisic acid (ABA) is an important plant hormone that mediates abiotic stresses in plant growth and development. A number of E3 ligases have been reported to be involved in ABA signaling pathway. In this study, we identified a C3H2C3 RING-type E3 ligase, Arabidopsis thaliana Tόxicos en Levadura 61 (ATL61), which regulated drought stress in planta. Enzyme assay in vitro demonstrated that ATL61 had E3 ubiquitin ligase activity, while point mutation of ATL61 (mATL61) abolished its E3 ubiquitin ligase activity. ATL61 overexpression plants exhibited ABA hypersensitivity and were more tolerant to drought, while the atl61 mutant plants were insensitive to ABA. Moreover, mATL61 overexpression lines exhibited similar ABA-related phenotypes with wild type (WT) plants. The transcript abundances of ABA-mediated drought stress-related genes RD20 and RD22 were higher in ATL61 overexpression plants than those in WT, atl61, and mATL61 plants. Our results indicated that ATL61 acted as a positive regulator in the ABA-mediated drought stress response.
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http://dx.doi.org/10.1016/j.bbrc.2020.05.067DOI Listing
July 2020

Biosynthesis of the starch is improved by the supplement of nickel (Ni) in duckweed (Landoltia punctata).

J Plant Res 2020 Jul 27;133(4):587-596. Epub 2020 May 27.

College of Life Sciences, Sichuan University , Chengdu, 610064, China.

Duckweed is a kind of floating aquatic plant and increasing its starch production is favorable for bioenergy. In this study, we found that starch biosynthesis was greatly promoted by the supplement of nickel ion (Ni) through the comparison of other different ions. The starch content in duckweed was increased by nearly eightfold when duckweed was treated with 20 µM Ni. The analysis of paraffin sections visually found that starch granules were more complete and dark blue in Ni treated duckweed than the control. Quantitative real-time PCR demonstrated that the expressions of starch synthesis-related enzymes were up-regulated in Ni treated duckweed. Further analysis revealed that the accumulation of Ni in duckweed effectively increased the activity of urease, which compensated for the deficiency of certain decrease in biomass and accelerated biosynthesis of the starch. Thus, our results represent another strategy to improve starch production of duckweed.
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http://dx.doi.org/10.1007/s10265-020-01204-0DOI Listing
July 2020

Insights into mechanism on organic acids assisted translocation of uranium in Brassica juncea var. foliosa by EXAFS.

J Environ Radioact 2020 Jul 29;218:106254. Epub 2020 Mar 29.

College of Chemistry, Sichuan University, Chengdu, 610064, China. Electronic address:

Citric acid (CA) and Lactic acid (LA) were used as additives to study the mechanism of organic acid promoting the root-to-shoot translocation of uranium (U) in Brassica juncea var. foliosa from molecular and tissue levels. Firstly, the distribution of U in plants under the condition of different organic acids concentrations were studied. The accumulation of U in leafs of 1 mM CA group and 5 mM LA group reached 2225 and 1848 mg/kg respectively, which was about 5 times that of the control group. Secondly, the speciation and distribution of U in plant roots after exposure to different culture solutions were studied by EXAFS and SEM. The result of EXAFS found that the complex of U with organic acids resulted in the U accumulated in the roots was the uranyl carboxylate speciation, while the control group only was the uranyl phosphate speciation. SEM results showed that the lactic acids could enhanced the translocation of U from the cortex to the stele. Thirdly, we further studied the apoplastic pathway and the symplastic pathway of U translocation using transpiration inhibitor and metabolism inhibitor. Compared with the control group, it was likely that the complex of U with organic acids were translocated into the shoot of plants through the apoplastic pathway.
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http://dx.doi.org/10.1016/j.jenvrad.2020.106254DOI Listing
July 2020

Incidence and Molecular Identification of Apple Necrotic Mosaic Virus (ApNMV) in Southwest China.

Plants (Basel) 2020 Mar 28;9(4). Epub 2020 Mar 28.

Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China.

Apple mosaic disease has a great influence on apple production. In this study, an investigation into the incidence of apple mosaic disease in southwest China was performed, and the pathogen associated with the disease was detected. The results show that 2869 apple trees with mosaic disease were found in the Sichuan, Yunnan, and Guizhou Provinces, with an average incidence of 9.6%. Although apple mosaic virus (ApMV) is widespread in apples worldwide, the diseased samples were negative when tested for ApMV. However, a novel ilarvirus (apple necrotic mosaic virus, ApNMV) was identified in mosaic apple leaves which tested negative for ApMV. RT-PCR analysis indicated that ApNMV was detected in 322 out of 357 samples with mosaic symptoms. Phylogenetic analysis of coat protein (CP) sequences of ApNMV isolates suggested that, compared with ApMV, ApNMV was closer to prunus necrotic ringspot virus (PNRSV). The CP sequences of the isolates showed the diversity of ApNMV, which may enable the virus to adapt to the changeable environments. In addition, the pathology of mosaic disease was observed by microscope, and the result showed that the arrangement of the tissue and the shape of the cell, including the organelle, were seriously destroyed or drastically changed.
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http://dx.doi.org/10.3390/plants9040415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7237995PMC
March 2020

Electrochemical investigation of the interactions of organic and inorganic depressants on basal and edge planes of molybdenite.

J Colloid Interface Sci 2020 Jun 6;570:350-361. Epub 2020 Mar 6.

Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB T6G 2V4, Canada. Electronic address:

Molybdenite (MoS) has attracted great attention in the field of advanced materials. MoS is normally separated from other minerals by selective flotation, and chemical reagents (so-called depressants) are required to lower the surface hydrophobicity of MoS. Previous studies had controversial arguments on the adsorption of organic and inorganic depressants, i.e., carboxymethyl cellulose (CMC), Ca and Mg on the anisotropic MoS surfaces. Therefore, in this work, the interactions of these depressants on MoS basal and edge surfaces in alkaline solutions were investigated using electrochemical techniques. The MoS edge surface had higher electrochemical reactivity than the basal surface, and the electrochemical reactivity of both the surfaces reduced after treatment in CMC, Ca and Mg solutions, which suggested that the depressants adsorbed on both basal and edge surfaces. The cyclic voltammetry and scanning electrochemical microscopy results indicated that the coverage of adsorbed inorganic species, i.e., Ca(OH), CaCO and Mg(OH) particles, on MoS basal and edge surfaces was lower than that of CMC. The randomly distributed hydrophilic Ca(OH), CaCO and Mg(OH) particles on MoS basal surface reduced the possibility of bubble attachment, which was the main reason for the depressed MoS floatability reported in the literature.
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http://dx.doi.org/10.1016/j.jcis.2020.03.007DOI Listing
June 2020

Longitudinal Analysis of Prevalence and Risk Factors of Rifampicin-Resistant Tuberculosis in Zhejiang, China.

Biomed Res Int 2020 12;2020:3159482. Epub 2020 Feb 12.

The Institute of TB Control, Zhejiang Provincial Center for Disease Control and Prevention, Zhejiang, China.

Objectives: To investigate the factors associated with rifampicin-resistant tuberculosis among drug resistant tuberculosis patients and to determine the correlation of rifampicin-resistant TB with MDR-TB in a high MDR-TB burden province of china.

Methods: A retrospective longitudinal analysis on four surveys of anti-TB drug resistance done in 1998, 2003, 2008, and 2013 in Zhejiang province, China. 4289 sputum-smear microscopy positive suspected tuberculosis patients were eligible at 30 investigation points, chosen by stratified random sampling at survey sites from all over the province. Culturing samples in L-J medium and the drug-susceptibility testing for the 4 first-line anti-TB drugs were performed to all patients. Multivariate logistic regression was carried out to determine the factors associated with the rifampicin-resistance in the study population.

Results: Overall, there were 3832 patients with positive mycobacterial cultures, and 2813 of the isolates (73.4%) were susceptible to all 4 first-line drugs. Analysis of rifampin monoresistant (RMR) TB indicated the prevalence was 1.1% in new cases and 3.4% in previously treated cases. Among the 359 rifampicin resistant TB (RR-TB) cases, 279 (77.7%) were also resistant to isoniazid, indicating MDR-TB. From 1998 to 2013, the proportion of MDR-TB among rifampicin-resistant TB cases varied between 80.0% and 87.5% (P for trend: 0.768) among previously treated cases and varied from 68.6% to 79.5% (P for trend: 0.403) among new cases. Among previously treated patients, those who received treatment for less than 6 months were less likely to have drug resistant TB (OR: 0.40, 95% CI: 0.16-0.97) or MDR-TB (OR: 0.24, 95% CI: 0.07-0.81). Patients who received anti-TB treatment in a general hospital were less likely to develop MDR-TB than those treated in a TB clinic (OR: 0.08, 95% CI: 0.01-0.72).

Conclusion: This study highlights a high proportion of RMR-TB among new RR-TB cases in Zhejiang, China. The management of treatment with rapid and accurate diagnosis of MDR-TB other than only relying on RIF susceptibility testing is crucial for improving adherence and outcomes in patients with drug-resistant TB.
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http://dx.doi.org/10.1155/2020/3159482DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037975PMC
November 2020

AtPPRT1 negatively regulates salt stress response in Arabidopsis seedlings.

Plant Signal Behav 2020 03 20;15(3):1732103. Epub 2020 Feb 20.

Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.

Salt stress is one of the environmental factors that negatively affect plant growth and development. We have previously reported a putative C3HC4 zinc-finger ubiquitin E3 ligase (AtPPRT1) negatively regulates Abscisic acid (ABA) and drought stress response. According to previous studies, the accumulation of ABA in plants can further regulate the salt stress response. Therefore, in this study, we further analyzed whether AtPPRT1 negatively regulates the salt stress response. The results showed that expression was induced by salt stress. Furthermore, under salt stress, the β-glucuronidase () gene driven by the promoter has shown increased activity in the hypocotyl and petioles of Arabidopsis seedlings. Additionally, seedlings of the T-DNA insertion mutant showed significant growth advantage under salt stress, whereas overexpressing (OE lines) in Arabidopsis seedlings displayed hypersensitive under salt stress. Etiolated seedlings also demonstrated significantly elongated hypocotyl lengths in salt stress. The elevated or reduced salt tolerance of and overexpressing lines was confirmed by the changes in chlorophyll content and 3,3'-Diaminobenzidine (DAB) staining. The above data suggest that AtPPRT1 has a negative effect on salt tolerance in Arabidopsis seedlings.
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http://dx.doi.org/10.1080/15592324.2020.1732103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7194377PMC
March 2020

Determination of veterinary drug/pesticide residues in livestock and poultry excrement using selective accelerated solvent extraction and magnetic material purification combined with ultra-high-performance liquid chromatography-tandem mass spectrometry.

J Chromatogr A 2020 Apr 19;1617:460808. Epub 2019 Dec 19.

A State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, PR China. Electronic address:

An ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method based on selective accelerated solvent extraction and magnetic material purification was established to analyze the residues of various veterinary antibiotics and agricultural fungicides and insecticides in livestock and poultry excrement. Methanol-acetonitrile (4:1, V/V) was used as the extraction solvent and static extraction was conducted three times in 5 min. Preliminary purification was achieved by adding 0.5 g acidic alumina-florisil (1:1, W/W) to the extraction cell while the extraction was conducted. This preliminarily-purified extract was further purified using magnetic material, then analyzed using UPLC-MS/MS. Under optimal conditions, 33 types of antibiotics, including 3 amphenicols, 8 macrolides, 17 sulfonamides and 5 nitroimidazoles, as well as 37 types of pesticides, including 27 insecticides and 10 fungicides, were detected. Recoveries ranged from 60.3% to 110.0% at three spiked concentrations (10 μg/kg, 20 μg/kg and 50 μg/kg), the detection limits ranged from 0.2 to 3.5 μg/kg and the quantitative limits ranged from 0.5 to 11.5 μg/kg. This newly-established method was tested using 30 livestock and poultry excrement samples and confirmed its use for determining veterinary drugs and pesticides in practical samples.
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http://dx.doi.org/10.1016/j.chroma.2019.460808DOI Listing
April 2020

DNA methylation profiling in recurrent miscarriage.

PeerJ 2020 7;8:e8196. Epub 2020 Jan 7.

NHC Key Lab. of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Medical School, Fudan University, Shanghai, China.

Recurrent miscarriage (RM) is a complex clinical problem. However, specific diagnostic biomarkers and candidate regulatory targets have not yet been identified. To explore RM-related biological markers and processes, we performed a genome-wide DNA methylation analysis using the Illumina Infinium HumanMethylation450 array platform. Methylation variable positions and differentially methylated regions (DMRs) were selected using the Limma package in R language. Thereafter, gene ontology (GO) enrichment analysis and pathway enrichment analysis were performed on these DMRs. A total of 1,799 DMRs were filtered out between patients with RM and healthy pregnant women. The GO terms were mainly related to system development, plasma membrane part, and sequence-specific DNA binding, while the enriched pathways included cell adhesion molecules, type I diabetes mellitus, and ECM-receptor interactions. In addition, genes, including , , , , and , were obtained. These genes may be potential candidates for diagnostic biomarkers and possible regulatory targets in RM. We then detected the mRNA expression levels of the candidate genes. The mRNA expression levels of the candidate genes in the RM group were significantly higher than those in the control group. However, additional research is still required to confirm their potential roles in the occurrence of RM.
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http://dx.doi.org/10.7717/peerj.8196DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953351PMC
January 2020