Publications by authors named "Jianliang Shen"

133 Publications

Effective tumor vessel barrier disruption mediated by perfluoro-N-(4-methylcyclohexyl) piperidine nanoparticles to enhance the efficacy of photodynamic therapy.

Nanoscale 2021 Aug 30;13(31):13473-13486. Epub 2021 Jul 30.

Department of Urology, Xiangya Hospital, Central South University, Changsha 410008, China.

Background: Currently, limited tumor drug permeation, poor oxygen perfusion and immunosuppressive microenvironments are the most important bottlenecks that significantly reduce the efficacy of photodynamic therapy (PDT). The main cause of these major bottlenecks is the platelet activation maintained abnormal tumor vessel barriers. Thus, platelet inhibition may present a new way to most effectively enhance the efficacy of PDT. However, to the best of our knowledge, few studies have validated the effectiveness of such a way in enhancing the efficacy of PDT both in vivo and in vitro. In this study, perfluoro-N-(4-methylcyclohexyl) piperidine-loaded albumin ([email protected]) nanoparticles were discovered, which possess excellent platelet inhibition ability. After [email protected] treatment, remarkably enhanced intra-tumoral drug accumulation, oxygen perfusion and T cell infiltration could be observed owing to the disrupted tumor vessel barriers. Besides, the effect of [email protected] mediated PDT was significantly amplified by [email protected] nanoparticles. It was demonstrated that [email protected] could be used as a useful tool to improve the efficacy of existing PDT by disrupting tumor vessel barriers through effective platelet inhibition.
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http://dx.doi.org/10.1039/d1nr02880dDOI Listing
August 2021

Loss of TRIM21 alleviates cardiotoxicity by suppressing ferroptosis induced by the chemotherapeutic agent doxorubicin.

EBioMedicine 2021 Jul 4;69:103456. Epub 2021 Jul 4.

Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, NJ, USA; Rutgers Cancer Institute of New Jersey, New Brunswick, NJ, USA. Electronic address:

Background: Doxorubicin, an anthracycline chemotherapeutic agent, is widely used in the treatment of many cancers. However, doxorubicin posts a great risk of adverse cardiovascular events, which are thought to be caused by oxidative stress. We recently reported that the ubiquitin E3 ligase TRIM21 interacts and ubiquitylates p62 and negatively regulates the p62-Keap1-Nrf2 antioxidant pathway. Therefore, we sought to determine the role TRIM21 in cardiotoxicity induced by oxidative damage.

Methods: Using TRIM21 knockout mice, we examined the effects of TRIM21 on cardiotoxicity induced by two oxidative damage models: the doxorubicin treatment model and the Left Anterior Descending (LAD) model. We also explored the underlying mechanism by RNA-sequencing of the heart tissues, and by treating the mouse embryonic fibroblasts (MEFs), immortalized rat cardiomyocyte line H9c2, and immortalized human cardiomyocyte line AC16 with doxorubicin.

Findings: TRIM21 knockout mice are protected from heart failure and fatality in both the doxorubicin and LAD models. Hearts of doxorubicin-treated wild-type mice exhibit deformed mitochondria and elevated level of lipid peroxidation reminiscent of ferroptosis, which is alleviated in TRIM21 knockout hearts. Mechanistically, TRIM21-deficient heart tissues and cultured MEFs and H9c2 cells display enhanced p62 sequestration of Keap1 and are protected from doxorubicin-induced ferroptosis. Reconstitution of wild-type but not the E3 ligase-dead and the p62 binding-deficient TRIM21 mutants impedes the protection from doxorubicin-induced cell death.

Interpretation: Our study demonstrates that TRIM21 ablation protects doxorubicin-induced cardiotoxicity and illustrates a new function of TRIM21 in ferroptosis, and suggests TRIM21 as a therapeutic target for reducing chemotherapy-related cardiotoxicity.

Funding: NIH (CA129536; DK108989): data collection, analysis. Shanghai Pujiang Program (19PJ1401900): data collection. National Natural Science Foundation (31971161): data collection. Department of Veteran Affairs (BX004083): data collection. Tianjin Science and Technology Plan Project (17ZXMFSY00020): data collection.
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http://dx.doi.org/10.1016/j.ebiom.2021.103456DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261003PMC
July 2021

Synergistic Activation of Antitumor Immunity by a Particulate Therapeutic Vaccine.

Adv Sci (Weinh) 2021 06 15;8(12):2100166. Epub 2021 Apr 15.

Department of Nanomedicine Houston Methodist Academic Institute Houston TX 77030 USA.

Success in anticancer immune therapy relies on stimulation of tumor antigen-specific T lymphocytes and effective infiltration of the T cells into tumor tissue. Here, a therapeutic vaccine that promotes proliferation and tumor infiltration of antigen-specific T cells in both inflamed and noninflamed tumor types is described. The vaccine consists of STING agonist 2'3'-cGAMP, TLR9 ligand CpG, and tumor antigen peptides that are loaded into nanoporous microparticles (GCVax). GCVax is effective in inhibiting lung metastatic melanoma, primary breast cancer, and subcutaneous colorectal cancer in their respective murine models, including functional cure of HER2-positive breast cancer. Mechanistically, GCVax potently stimulates type I interferon expression in dendritic cells, and promotes CD8 and CD103 dendritic cell maturation and migration to lymph nodes and other lymphatic tissues. Antitumor responses are dependent on TLR9 and interferon / receptor signaling, and to a less extent on STING signaling. These results demonstrate a high potential for GCVax in mediating antitumor immunity in personalized cancer therapy.
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http://dx.doi.org/10.1002/advs.202100166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224417PMC
June 2021

Preoperative histogram parameters of dynamic contrast-enhanced MRI as a potential imaging biomarker for assessing the expression of Ki-67 in prostate cancer.

Cancer Med 2021 07 12;10(13):4240-4249. Epub 2021 Jun 12.

Department of Radiology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.

Purpose: To investigate whether preoperative histogram parameters of dynamic contrast-enhanced MRI (DCE-MRI) can assess the expression of Ki-67 in prostate cancer (PCa).

Materials And Methods: A consecutive series of 76 patients with pathology-proven PCa who underwent routine DCE-MRI scans were retrospectively recruited. Quantitative parameters including the volume transfer constant (K ), rate contrast (K ), extracellular-extravascular volume fraction (V ), and plasma volume (V ) by outlining the three-dimensional volume of interest (VOI) of all lesions were processed. Then, the histogram analyses of these quantitative parameters were performed. The Spearman rank correlation analysis was used to evaluate the correlation of these parameters and Ki-67 expression of PCa. Receiver operating characteristic (ROC) curve analysis was adopted to evaluate the efficacy of these quantitative histogram parameters in identifying high Ki-67 expression from low Ki-67 expression of PCa.

Results: Eighty-eight PCa lesions were enrolled in this study, including 31 lesions with high Ki-67 expression and 57 lesions with low Ki-67 expression. The median, mean, 75th percentile, and 90th percentile derived from K and K had a moderately positive correlation with Ki-67 expression (r = 0.361-0.450, p < 0.05), in which both the median and mean of K had the highest positive correlation (r = 0.450, p < 0.05). The diagnostic efficacy of the K median, mean, 75th percentile, and 90th percentile, along with the K -based median and mean was assessed by the ROC curve. The area under the curve (AUC) of the mean for K was the highest (0.826). When the cut-off of the mean for K was ≥0.47/min, its Youden index, sensitivity, and specificity were 0.625, 0.871, and 0.754, respectively. The AUC of the median of K was the lowest (0.772).

Conclusion: The histogram of DCE-MRI quantitative parameters is correlated with Ki-67 expression, which has the potential to noninvasively assess the expression of Ki-67 with patients of PCa.
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http://dx.doi.org/10.1002/cam4.3912DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267123PMC
July 2021

ε‑Polylysine-stabilized agarose/polydopamine hydrogel dressings with robust photothermal property for wound healing.

Carbohydr Polym 2021 Jul 7;264:118046. Epub 2021 Apr 7.

State Key Laboratory of Ophthalmology, Optometry and Vision Science, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, 325027, China; Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Xinsan Road, Longwan District, Wenzhou, 325001, China. Electronic address:

Polydopamine (PDA) is emerging as an attractive photothermal agent due to its good photothermal performance and excellent biocompatibility. However, without chemical modification, PDA is normally unstable and usually leached out from the constructed biomaterials, realistically limiting its application space. Here, we constructed a new hydrogel dressing with robust and stable photothermal performance by introduction of ε-Polylysine (ε-PL) into agarose/PDA matrix to efficiently lock PDA. By optimizing PDA/ε-PL rational dose in agarose network structure, a hybrid agarose/PDA/ε-PL hydrogel (ADPH) with stable photothermal functionality and desirable physicochemical properties could be achieved. ADPH possessed satisfactory microbicidal efficacy in vivo, which enabled the bacteria-infected skin wound to be cured quickly by successful suppressing inflammation, accelerating collagen deposition and promoting angiogenesis in a bacterial-infected wound model. Collectively, this study illustrates a simple, convenient but powerful strategy to design functionally stable ADPH dressing for treating dermal wounds, which could open vistas in clinical wound management.
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http://dx.doi.org/10.1016/j.carbpol.2021.118046DOI Listing
July 2021

Electrostatic-modulated interfacial polymerization toward ultra-permselective nanofiltration membranes.

iScience 2021 Apr 26;24(4):102369. Epub 2021 Mar 26.

Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.

Interfacial polymerization (IP) is a platform technology for ultrathin membranes. However, most efforts in regulating the IP process have been focused on short-range H-bond interaction, often leading to low-permselective membranes. Herein, we report an electrostatic-modulated interfacial polymerization (eIP) supercharged phosphate-rich substrates toward ultra-permselective polyamide membranes. Phytate, a natural strongly charged organophosphate, confers high-density long-range electrostatic attraction to aqueous monomers and affords tunable charge density by flexible metal-organophosphate coordination. The electrostatic attraction spatially enriches amine monomers and temporally decelerates their diffusion into organic phase to be polymerized with acyl chloride monomers, triggering membrane sealing and inhibiting membrane growth, thus generating polyamide membranes with reduced thickness and enhanced cross-linking. The optimized nearly 10-nm-thick and highly cross-linked polyamide membrane displays superior water permeance and ionic selectivity. This eIP approach is applicable to the majority of conventional IP processes and can be extended to fabricate a variety of advanced membranes from polymers, supermolecules, and organic framework materials.
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http://dx.doi.org/10.1016/j.isci.2021.102369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059057PMC
April 2021

A novel ratiometric and colorimetric chemosensor for highly sensitive, selective and ultrafast tracing of HClO in live cells, bacteria and zebrafish.

Anal Chim Acta 2021 May 5;1161:338472. Epub 2021 Apr 5.

School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China. Electronic address:

Hypochlorous acid (HClO) along with its ionic form, hypochlorite anion (ClO) are critical reactive oxygen species (ROS), which play vital roles in biological systems. Dysregulated production of HClO/ClO can result in tissue damage and cause a variety of diseases. Besides, Sodium hypochlorite has been widely used as a bleaching agent for water disinfection, surface cleaning in daily life. Excessive exposure to sodium hypochlorite will lead to symptoms of severe breathing and skin problems. Therefore, developing a state-of-the-art (simple, highly sensitive, highly selective and super fast-response) sensor for tracking HClO is of biological, toxicological, and environmental importance. Though many HClO probes have been reported so far, this big aim still presents a challenge. Researchers around the world are continuing to develop new HClO probes that could improve their sensitivity, selectivity, the limit of detection, response time, easiness to use, etc. Herein, with coumarin as the fluorophore molecule, we rationally developed a novel chemosensor (CMTH) for detecting HClO with both ratiometric and colorimetric responses resulted from the oxidation reaction of CN bond. Further analysis results indicated that CMTH can realize highly sensitive with low limit of detection (256 nM, among the best of its kind) and highly selective (over a bunch of interfering analytes) imaging detection of HClO in multiple organisms with low cytotoxicity, and good cell and tissue permeability as well. In particular, compared to other fluorescent HClO probes reported so far, CMTH excels in the response time to HClO (< 40 s), being the top-notch of its kind. Besides, owing to its excellent water solubility, CMTH can also be applied to track HClO in the environmental system. Taken together, we have presented here a novel chemosensor, CMTH, as a colorimetric and ratiometric chemosensor for highly sensitive and ultrafast imaging detection of HClO in aqueous solutions, eukaryotic cells, prokaryotic bacteria and vertebrate zebrafish.
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http://dx.doi.org/10.1016/j.aca.2021.338472DOI Listing
May 2021

Highly sensitive fluorescent sensor based on coumarin organic dye for pyrophosphate ion turn-on biosensing in synovial fluid.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Aug 8;257:119792. Epub 2021 Apr 8.

State Key Laboratory of Ophthalmology, Optometry and Vision Science, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou 325035, PR China; Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Science, Wenzhou 325001, PR China. Electronic address:

Highly sensitive fluorescence detection of pyrophosphate ion (PPi) is in urgent demand but remains a great obstacle, ascribing to scarcity of high-performance materials with promising optical property and high affinity. Herein, we report the design and fabrication of a coumarin-based organic dye (DCCH-TPD) containing both hydrazide group and terpyridine moiety for PPi biosensing through Cu-induced photo-electron transfer (PET) effect and target analyte-switched competitive coordination reaction. Individual DCCH-TPD was found to be highly emissive, and displayed a turn-off response toward Cu due to formation of [email protected] and PET effect. The recognition of [email protected] by PPi leads to generation of [email protected] complex, which greatly reduces the amount of Cu coordinated with DCCH-TPD, subsequently decreasing PET effect. Significantly enhanced fluorescence is recorded and the fluorescence intensity is closely relied on PPi concentration. Thus, highly sensitive detection of PPi is achieved, and the detection limit was calculated to be 0.075 μM. Furthermore, the proposed sensor presented good selectivity, and excellent practical ability for application in arthritic fluid.
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http://dx.doi.org/10.1016/j.saa.2021.119792DOI Listing
August 2021

Retraction: Size-selected silver nanoparticles for MALDI-TOF mass spectrometry of amyloid-beta peptides.

Nanoscale 2021 Apr;13(16):7862

School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China and Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Science, Wenzhou, Zhejiang, China.

Retraction of 'Size-selected silver nanoparticles for MALDI-TOF mass spectrometry of amyloid-beta peptides' by Feng Ding et al., Nanoscale, 2018, 10, 22044-22054, DOI: 10.1039/C8NR07921H.
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http://dx.doi.org/10.1039/d1nr90081aDOI Listing
April 2021

Reaction-based chemosensor as dual-channel indicator for visualizing and bioimaging of exogenous hypochlorite concentrations in living cells, Pseudomonas aeruginosa, and zebrafish.

Anal Chim Acta 2021 May 12;1157:338391. Epub 2021 Mar 12.

School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China; Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, 325001, China. Electronic address:

Tracking and quantifying hypochlorite (ClO) in biological systems and environments remain challenging tasks, and many efforts have been made to improve ClO recognition performance by modifying the sensor structure. In this study, a pre-designed coumarin/furanohydrazide-based sensor (CMFH) with the coumarin moiety as the building block (fluorogen) was rationally prepared as a ratiometric and colorimetric chemosensor for ClO recognition. As expected, CMFH demonstrated excellent sensitivity and selectivity for ClO detection. The fluorescence signal ratio (F/F) showed strong ClO dependency, and the sensor exhibited ultrafast detection (within 60 s) and a low detection limit of 563 nM. Due to its low cytotoxicity and good tissue permeability, CMFH was demonstrated as a dual-channel sensor for ClO bioimaging and visualization in cells, zebrafish, and even bacteria. Furthermore, CMFH-loaded paper strips were successfully applied to the colorimetric and fluorescent visualization of ClO. The results demonstrate that CMFH has potential application value for tracking ClO in various biosystems and environments.
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http://dx.doi.org/10.1016/j.aca.2021.338391DOI Listing
May 2021

3D CoPt nanostructures hybridized with iridium complexes for multimodal imaging and combined photothermal-chemotherapy.

J Inorg Biochem 2021 Jun 19;219:111429. Epub 2021 Mar 19.

MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, China. Electronic address:

Combined photothermal-chemotherapy has shown great potential in improving the efficiency of tumor treatment. In this article, we have designed a new type of nanocomposite Ir-CoPt-PVP composed of cobalt/platinum alloy nanoparticles (CoPt) and iridium(III) complex (Ir) for combined photothermal therapy (PTT) and chemotherapy. The obtained CoPt was synthesized by a simple solvothermal method and modified by polyvinyl pyrrolidone (PVP), which exhibited excellent photothermal efficiency and stability, and can also be a bimodal bioimaging contrast agent in photothermal imaging (PTI) and photoacoustic imaging (PAI). Furthermore, the combination therapy has shown obvious tumor cell-growth inhibition in vitro. Overall, the results revealed the great potential of Ir-CoPt-PVP nanocomposites in improving therapeutic efficiency by photothermal-chemotherapy and photothermal/photoacoustic imaging.
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http://dx.doi.org/10.1016/j.jinorgbio.2021.111429DOI Listing
June 2021

A newly nitrobenzoxadiazole (NBD)-fused reversible fluorescence probe for pH monitoring and application in bioimaging.

Talanta 2021 Jun 19;228:122218. Epub 2021 Feb 19.

Ningbo Eye Hospital, Ningbo, Zhejiang, 315040, China; School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China. Electronic address:

pH plays an essential role in virtually all biological processes, which thus needs to be tightly regulated. Especially small changes in pH value in biological systems will affect the normal metabolic functions of animals and plants. Therefore, it is very important to accurately track changes in pH in biological systems. Herein, we rationally fabricated a new molecular pH probe (NBD-pbz) based on conjugation of the 2-Piperazin-1-yl-1,3-benzothiazole (pbz) and 7-nitro-1,2,3-benzoxadiazole (NBD) building blocks. The following test on NBD-pbz revealed that it can offer a sensitive and selective tracking of pH changes from 3.2 to 7.6 with pK 5.51, and not only in eukaryotic cells (especially the imaging of ocular tumor cell OCM-1) and zebrafish but also in mung bean sprouts via fluorometric turn on response and an intramolecular charge transfer (ICT) based working mechanism strategy. Therefore, this NBD-pbz probe not only replenishes the current repertory of molecular pH probes but also extends the application of pH probes to monitor pH variation in the plant kingdom, which will undoubtedly arouse greater research interest in the field.
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http://dx.doi.org/10.1016/j.talanta.2021.122218DOI Listing
June 2021

Reversible Chemosensor for Bioimaging and Biosensing of Zn(II) and hpH in Cells, Larval Zebrafish, and Plants with Dual-Channel Fluorescence Signals.

Inorg Chem 2021 Apr 5;60(8):5563-5572. Epub 2021 Mar 5.

School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.

Zinc/Zn(II) is an essential trace element for humans and acts as an important substance that maintains the normal growth, development, and metabolism of the body. Excess or deficient Zn(II) can cause abnormal metabolism in the human body, leading to a series of diseases. Moreover, biosystems have complex homeostasis systems, especially harsh pH (OH) environments. Thus, investigating the variation in the levels of Zn(II) and OH is extremely important in clinical, medical, and environmental testing. Nevertheless, the lack of practical and convenient fluorescence imaging tools limits the tracing of Zn(II) and OH in biosystems. In this work, a well-designed dual-channel fluorescent signal response chemosensor () was assembled for selective sensing of Zn(II) and OH in the biosystem using a fluorescence turn-on strategy. On encountering Zn(II), the chemosensor emitted a blue fluorescence signal (455 nm). Meanwhile, the bright green fluorescence signal (530 nm) increased with OH addition simultaneously. With the blue/green dual fluorescence response of , the sensor exhibited high stability and reversibility. Notably, the bioimaging revealed that successfully tracked Zn(II) and OH in live cells, larval zebrafish, and plants. Further results implied that can be used to achieve visual detection of Zn(II) and OH in organisms. Altogether, this work is conducive to the monitoring of Zn(II) and OH in organisms and promotes the understanding of the function of Zn(II) and OH in biosystems.
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http://dx.doi.org/10.1021/acs.inorgchem.0c03456DOI Listing
April 2021

Polydopamine nanoparticle-dotted food gum hydrogel with excellent antibacterial activity and rapid shape adaptability for accelerated bacteria-infected wound healing.

Bioact Mater 2021 Sep 12;6(9):2647-2657. Epub 2021 Feb 12.

State Key Laboratory of Ophthalmology, Optometry and Vision Science, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China.

Most commonly used wound dressings have severe problems, such as an inability to adapt to wound shape or a lack of antibacterial capacity, affecting their ability to meet the requirements of clinical applications. Here, a nanocomposite hydrogel (XKP) is developed by introducing polydopamine nanoparticles (PDA NPs) into a food gum matrix (XK, consisting of xanthan gum and konjac glucomannan, both FDA-approved food thickening agents) for skin wound healing. In this system, the embedded PDA NPs not only interact with the food gum matrix to form a hydrogel with excellent mechanical strength, but also act as photothermal transduction agents to convert near-infrared laser radiation to heat, thereby triggering bacterial death. Moreover, the XKP hydrogel has high elasticity and tunable water content, enabling it to adapt to the shape of the wound and insulate it, providing a moist environment suitable for healing. skin wound healing results clearly demonstrate that XKP can significantly accelerate the healing of wounds by reducing the inflammatory response and promoting vascular reconstruction. In summary, this strategy provides a simple and practical method to overcome the drawbacks of traditional wound dressings, and provides further options when choosing suitable wound healing materials for clinical applications.
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http://dx.doi.org/10.1016/j.bioactmat.2021.01.035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890098PMC
September 2021

ESIPT-based fluorescent probe for bioimaging and identification of group IIIA ions in live cells and zebrafish.

Bioorg Chem 2021 04 20;109:104746. Epub 2021 Feb 20.

School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China. Electronic address:

Aluminum (Al), gallium (Ga), indium (In) are three essential elements in group IIIA of the periodic table, which all share similar chemical properties and are also vital in many aspects of bio- and environmental systems. Proper control of their levels is thus necessary as overexposure to them has been linked to onsets of many diseases. Fluorescence based molecular probes have always been the driving horse for detecting vital ions including group IIIA ions. However, only a few such probes have been reported so far and all of them are faced with one or more shortcomings such as not very high sensitivity, incapability to detect multiple ions simultaneously, and poor cell penetration abilities due to emitted fluorescence at shorter wavelengths. To meet those challenges, we herein presented the successful development and application of a novel group IIIA ions fluorescent probe, NBD-hnap, in live RAW264.7 cell and zebrafish models, especially the imaging of ocular tumor cell OCM-1 (human choroid melanoma cells). NBD-hnap was synthesized by a simple conjugation of NBD and hnap molecules under suitable conditions. Subsequent experimental analysis and theoretical calculations confirmed that NBD-hnap forms a 1:1 chelate with each of three selected group IIIA ions. Further evaluation proved that NBD-hnap can realize highly sensitive [LODs of 113, 82 and 150 nM for Al(III), Ga(III), and In(III) respectively in aqueous solutions] and highly selective (over a dozen of interfering cations) through an ESIPT-based fluorescent sensing mechanism with strong far-red emission around 640 nm. Those value merits make NBD-hnap superior to other group IIIA ion probes reported before and NBD-hnap is thus expected to find wider and greater applications in the near future.
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http://dx.doi.org/10.1016/j.bioorg.2021.104746DOI Listing
April 2021

Metformin Liposome-Mediated PD-L1 Downregulation for Amplifying the Photodynamic Immunotherapy Efficacy.

ACS Appl Mater Interfaces 2021 Feb 12;13(7):8026-8041. Epub 2021 Feb 12.

State Key Laboratory of Ophthalmology, Optometry and Vision Science, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou 325027, China.

Photodynamic therapy (PDT) is a promising strategy for cancer treatment. It can not only generate reactive oxygen species (ROS) to cause the chemical damage of tumor cells in the presence of enough oxygen but also promote the antitumor immunity of T cells through enhancing the production of interferon γ (IFN-γ). However, one phenomenon is ignored so far that the enhanced production of IFN-γ caused by PDT may significantly increase the expression of programmed death-ligand 1 (PD-L1) on the tumor cell membrane and thus could inhibit the immune killing effects of T cells. Herein, we report the construction of a composite by loading metformin (Met) and IR775 into a clinically usable liposome as a two-in-one nanoplatform ([email protected]@Lip) to solve this problem. The [email protected]@Lip could reverse tumor hypoxia to enhance ROS production to elicit more chemical damage. Besides, the overexpression of PD-L1 by PDT was also effectively down-regulated. These therapeutic benefits including decreased PD-L1 expression, alleviated T cell exhaustion, and reversed tumor hypoxia successfully suppressed both the primary and abscopal tumor growth in bladder and colon cancers, respectively. Combining with its excellent biocompatibility, our results indicate that this [email protected]@Lip system has great potential to become a highly effective cancer therapy modality.
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http://dx.doi.org/10.1021/acsami.0c21743DOI Listing
February 2021

Constructing Cylindrical Nanostructures Via Directional Morphology Evolution Induced by Seeded Polymerization.

Macromol Rapid Commun 2021 May 5;42(9):e2100001. Epub 2021 Feb 5.

State Key Laboratory of Ophthalmology, Optometry and Vision Science, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, 325027, P. R. China.

Herein, spindle-shaped block copolymer (BCP) nanoparticles are used in seeded polymerization of methyl methacrylate as a novel approach to generating cylindrical nanostructures. The chain-extension of BCP seeds by an amorphous coil-type polymer within the seed core composed of semifluorinated liquid-crystalline blocks triggers the deforming, stretching, and directional growth of the seeds along the long axis, eventually leads to nanorods.
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http://dx.doi.org/10.1002/marc.202100001DOI Listing
May 2021

The Ubiquitin E3 Ligase TRIM21 Promotes Hepatocarcinogenesis by Suppressing the p62-Keap1-Nrf2 Antioxidant Pathway.

Cell Mol Gastroenterol Hepatol 2021 19;11(5):1369-1385. Epub 2021 Jan 19.

Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, New Jersey; Cancer Metabolism and Growth Program, Rutgers Cancer Institute of New Jersey, New Brunswick, New Jersey. Electronic address:

Background And Aims: TRIM21 is a ubiquitin E3 ligase that is implicated in numerous biological processes including immune response, cell metabolism, redox homeostasis, and cancer development. We recently reported that TRIM21 can negatively regulate the p62-Keap1-Nrf2 antioxidant pathway by ubiquitylating p62 and prevents its oligomerization and protein sequestration function. As redox homeostasis plays a pivotal role in many cancers including liver cancer, we sought to determine the role of TRIM21 in hepatocarcinogenesis.

Methods: We examined the correlation between TRIM21 expression and the disease using publicly available data sets and 49 cases of HCC clinical samples. We used TRIM21 genetic knockout mice to determine how TRIM21 ablation impact HCC induced by the carcinogen DEN plus phenobarbital (PB). We explored the mechanism that loss of TRIM21 protects cells from DEN-induced oxidative damage and cell death.

Results: There is a positive correlation between TRIM21 expression and HCC. Consistently, TRIM21-knockout mice are resistant to DEN-induced hepatocarcinogenesis. This is accompanied by decreased cell death and tissue damage upon DEN treatment, hence reduced hepatic tissue repair response and compensatory proliferation. Cells deficient in TRIM21 display enhanced p62 sequestration of Keap1 and are protected from DEN-induced ROS induction and cell death. Reconstitution of wild-type but not the E3 ligase-dead and the p62 binding-deficient mutant TRIM21 impedes the protection from DEN-induced oxidative damage and cell death in TRIM21-deficient cells.

Conclusions: Increased TRIM21 expression is associated with human HCC. Genetic ablation of TRIM21 leads to protection against oxidative hepatic damage and decreased hepatocarcinogenesis, suggesting TRIM21 as a preventive and therapeutic target.
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http://dx.doi.org/10.1016/j.jcmgh.2021.01.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024979PMC
January 2021

Development of targeted therapy therapeutics to sensitize triple-negative breast cancer chemosensitivity utilizing bacteriophage phi29 derived packaging RNA.

J Nanobiotechnology 2021 Jan 7;19(1):13. Epub 2021 Jan 7.

School of Biomedical Engineering, School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, 325035, Zhejiang, People's Republic of China.

Background: To date, triple-negative breast cancer (TNBC) treatment options are limited because of the loss of target receptors and, as a result, are only managed with chemotherapy. What is worse is that TNBC is frequently developing resistance to chemotherapy. By using small interfering RNA (siRNA)-based therapeutics, our recent work demonstrated X-box-binding protein 1 (XBP1) was linked to human epidermal growth factor receptor 2 positive (HER2+) breast cancer development and chemoresistance. Given the instability, off-target effects, net negative charge, and hydrophobicity of siRNA in vivo utilization and clinical transformation, its use in treatment is hampered. Thus, the development of a siRNA-based drug delivery system (DDS) with ultra-stability and specificity is necessary to address the predicament of siRNA delivery.

Results: Here, we assembled RNase resistant RNA nanoparticles (NPs) based on the 3WJ structure from Phi29 DNA packaging motor. To improved targeted therapy and sensitize TNBC to chemotherapy, the RNA NPs were equipped with an epidermal growth factor receptor (EGFR) targeting aptamer and XBP1 siRNA. We found our RNA NPs could deplete XBP1 expression and suppress tumor growth after intravenous administration. Meanwhile, RNA NPs treatment could promote sensitization to chemotherapy and impede angiogenesis in vivo.

Conclusions: The results further demonstrate that our RNA NPs could serve as an effective and promising platform not only for siRNA delivery but also for chemotherapy-resistant TNBC therapy.
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http://dx.doi.org/10.1186/s12951-020-00758-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7792131PMC
January 2021

Editorial: Supramolecular Nanomaterials for Engineering, Drug Delivery, and Medical Applications.

Front Chem 2020 9;8:626468. Epub 2020 Dec 9.

Université de Paris, CNRS, Inserm, UTCBS, Chemical and Biological Technologies for Health Group (utcbs.cnrs.fr), Faculté de Pharmacie, Paris, France.

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http://dx.doi.org/10.3389/fchem.2020.626468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755928PMC
December 2020

Hydrogen in Patients With Corticosteroid-Refractory/Dependent Chronic Graft-Versus-Host-Disease: A Single-Arm, Multicenter, Open-Label, Phase 2 Trial.

Front Immunol 2020 25;11:598359. Epub 2020 Nov 25.

Department of Hematology, Boren Hospital, Beijing, China.

Chronic graft-versus-host-disease (cGVHD) is the leading cause of late non-relapse mortality after allogeneic hematopoietic stem cell transplantation(HSCT). There is no standard therapy for patients refractory or dependent to corticosteroid treatment. We hypothesized that hydrogen may exert therapeutic effects on cGVHD patients with few side effects. A prospective open-label phase 2 study of hydrogen was conducted. Patients received hydrogen-rich water 4ml/kg orally three times a day. Responses were graded in the skin, mouth, Gastrointestinal(GI), liver, eyes, lungs and joints and fascia every 3 months. A total of 24 patients (median age 27) were enrolled. Of the 24 patients, 18 (75%; 95% CI, 55.1% to 88%) had an objective response. No significant toxicity was observed. The estimated 4-year overall survival rate was 74.7%(95% CI, 54.9%-94.5%). The survival time was significantly prolonged in the response group. The survival rate at 4 years in the response group is significantly higher than the nonresponse group (86.6% vs 0%; p= 0.000132). Hydrogen showed great efficacy on cGVHD patients and long-term administration of hydrogen was not associated with significant toxic effects. The trial was registered at www.ClinicalTrials.Gov, NCT02918188.
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http://dx.doi.org/10.3389/fimmu.2020.598359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724019PMC
June 2021

Polydopamine-incorporated dextran hydrogel drug carrier with tailorable structure for wound healing.

Carbohydr Polym 2021 Feb 13;253:117213. Epub 2020 Oct 13.

State Key Laboratory of Ophthalmology, Optometry and Vision Science, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, 325027, China; Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, 325001, China. Electronic address:

Effective methods to treat bacterial infections are highly desired as the abuse of antibiotics has caused multidrug-resistant. Polysaccharide hydrogel-based drug delivery systems possessing inherent large surface area and biocompatibility attributes provide a promising strategy for effective use of antibiotics. Here, we presented an effective method for fabricating macroporous polysaccharide hydrogels composed of dextran (DP) and polydopamine (PDA) for controlled release of antibiotics. The physicochemical properties of resulting DP hydrogels were systematically evaluated by measuring their swelling, viscoelasticity, morphology, sorption and thermal stability, and we could control these properties through simply changing the PDA concentration in a pre-gel solution. The low cytotoxicity of DP hydrogels was demonstrated through a co-culture with mouse fibroblast cells. Moreover, in vitro/vivo antibacterial properties of the drug-loading DP hydrogels were evaluated, and they exhibited good antibacterial and healing performances. We believe that the proposed strategy for facilitation and optimization of polysaccharide hydrogels could offer more hydrogel dressings when choosing suitable carriers for sustained release of antibiotics.
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http://dx.doi.org/10.1016/j.carbpol.2020.117213DOI Listing
February 2021

Mussel-inspired agarose hydrogel scaffolds for skin tissue engineering.

Bioact Mater 2021 Mar 17;6(3):579-588. Epub 2020 Sep 17.

State Key Laboratory of Ophthalmology, Optometry and Vision Science, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, 325027, China.

Polysaccharide hydrogels are widely used in tissue engineering because of their superior biocompatibility and low immunogenicity. However, many of these hydrogels are unrealistic for practical applications as the cost of raw materials is high, and the fabrication steps are tedious. This study focuses on the facile fabrication and optimization of agarose-polydopamine hydrogel (APG) scaffolds for skin wound healing. The first study objective was to evaluate the effects of polydopamine (PDA) on the mechanical properties, water holding capacity and cell adhesiveness of APG. We observed that APG showed decreased rigidity and increased water content with the addition of PDA. Most importantly, decreased rigidity translated into significant increase in cell adhesiveness. Next, the slow biodegradability and high biocompatibility of APG with the highest PDA content (APG3) was confirmed. In addition, APG3 promoted full-thickness skin defect healing by accelerating collagen deposition and promoting angiogenesis. Altogether, we have developed a straightforward and efficient strategy to construct functional APG scaffold for skin tissue engineering, which has translation potentials in clinical practice.
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http://dx.doi.org/10.1016/j.bioactmat.2020.09.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509181PMC
March 2021

Reaction-Based Ratiometric and Colorimetric Chemosensor for Bioimaging of Biosulfite in Live Cells, Zebrafish, and Food Samples.

J Agric Food Chem 2020 Oct 14;68(42):11774-11781. Epub 2020 Sep 14.

School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.

In this work, a reaction-based ratiometric and colorimetric sensor was designed and synthesized for probing bisulfite (HSO) by coupling coumarin () with barbituric () moiety. Further tests have shown that has high selectivity and sensitivity for the recognition of HSO, which can be applied for the detection of HSO in environmental and biological systems very effectively. The fluorescence intensity ratios (/) exhibited an outstanding HSO-dependent response with ultrafast response time (within 20 s) and a lower detection limit (105 nM). Meanwhile, the color of the solution changed from green to colorless during the recognition process, and its fluorescence changed from green to blue. The mechanism of response is confirmed by the density functional theory (DFT) model. In summary, has demonstrated low toxicity and good permeability, which can be applied for imaging HSO in cells and zebrafish safely and effectively. Besides, this novel sensor successfully realized the quantification of the concentration of HSO in paper strips and food samples.
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http://dx.doi.org/10.1021/acs.jafc.0c03983DOI Listing
October 2020

Chidamide, decitabine, cytarabine, aclarubicin, and granulocyte colony-stimulating factor (CDCAG) in patients with relapsed/refractory acute myeloid leukemia: a single-arm, phase 1/2 study.

Clin Epigenetics 2020 09 1;12(1):132. Epub 2020 Sep 1.

Department of Hematology-Oncology, International Cancer Center, Shenzhen University General Hospital, Shenzhen University Health Science Center, Shenzhen, China.

Background: Epigenetic mechanisms play an important role in the chemoresistance of acute myeloid leukemia (AML). The clinical response to epigenetic modifier-based chemotherapy in patients with relapsed/refractory AML (r/r AML) is unclear. This multicenter clinical trial evaluated the safety and efficacy of epigenetic modifiers (chidamide and decitabine) in combination with aclarubicin, cytarabine, and granulocyte colony-stimulating factor (G-CSF) in patients with r/r AML.

Results: Adult patients with r/r AML were treated with chidamide, decitabine, cytarabine, aclarubicin, and G-CSF (CDCAG). The primary measures were overall response (OR), overall survival (OS), and safety. Next-generation sequencing was performed to analyze the correlation between gene mutations and response. A total of 93 patients with r/r AML were enrolled. Overall, 24 patients had a complete remission (CR) and 19 patients achieved CR with incomplete blood count recovery (CRi). The overall response rate (ORR) was 46.2%. The overall survival of these 43 patients who achieved CR/CRi was significantly longer than that of patients who failed to achieve remission (563 vs 152 days, P < 0.0001). Of the patients with mutations in epigenetic and transcription factor-related genes, but without internal tandem duplications in FMS-like tyrosine kinase3 (FLT3-ITDs), 55.6% achieved CR/CRi, whereas the ORR was 28.2% for patients with mutations in other genes.

Conclusions: The CDCAG regimen was well tolerated and effective in r/r AML. Patients with epigenetic and transcription factor-related gene mutations, but without FLT3-ITD mutations, may benefit from this regimen.

Trial Registration: Clinical Trials, NCT02886559 . Registered 01 September 2016.
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http://dx.doi.org/10.1186/s13148-020-00923-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466805PMC
September 2020

FRET-based colorimetric and ratiometric sensor for visualizing pH change and application for bioimaging in living cells, bacteria and zebrafish.

Anal Chim Acta 2020 Aug 30;1127:29-38. Epub 2020 Jun 30.

School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China; Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, 325001, China. Electronic address:

Acid-alkaline balance plays a crucial role in all biological processes. Accordingly, monitoring pH changes will help us to understand the functional status of these physiological and pathological processes. Though fluorescent probes may be a useful tool for detecting pH changes, and there are many limitations to currently available probes, such as background interference, potential cytotoxicity, and poor cell permeability, which call for a solution urgently. In this work, a rhodamine-derived colorimetric and ratiometric sensor (Rh-HN) was fabricated for monitoring pH change via the mechanism of fluorescence resonance energy transfer (FRET). Rh-HN has been shown to possess several advantages over other probes, such as high sensitivity, outstanding permeability, and low toxicity. Besides, the fluorescence intensity ratio (F/F) of Rh-HN displays a pH-sensitive response from 2.0 to 7.5 (pKa = 5.05) and linear response from pH 3.8 to 6.4, which was desirable for mapping pH change in the biological systems. Besides, the results indicated that Rh-HN generated a pH-dependent response regulated by switchable forms between closed and opened spirolactam ring. Overall, Rh-HN has accomplished sensing and mapping of pH in living cells, bacteria, and zebrafish. Those results demonstrated that the great potential of Rh-HN in sensing and visualizing pH in the living biosystem.
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http://dx.doi.org/10.1016/j.aca.2020.06.031DOI Listing
August 2020

Polydopamine/montmorillonite-embedded pullulan hydrogels as efficient adsorbents for removing crystal violet.

J Hazard Mater 2021 01 2;402:123359. Epub 2020 Jul 2.

State Key Laboratory of Ophthalmology, Optometry and Vision Science, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, 325027, China; Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, 325001, China. Electronic address:

Both secondary pollution and the low mechanical strength of adsorbents have severely impeded the practical application of adsorption methods in the dye wastewater treatment. In this work, we innovatively synthesized a composite hydrogel adsorbent by incorporating polydopamine (PDA) and montmorillonite (MMT) into the pullulan hydrogel matrix for dye adsorption. First, the successful formation of the resultant adsorbents was verified by Fourier-transform infrared spectroscopy and scanning electron microscope elemental mapping analysis. Then, several physicochemical properties (such as thermal and swelling properties, microarchitecture, and mechanical strength) of the five prepared adsorbents (PM1-PM5) were investigated. These results demonstrated the adsorbents had tunable properties, which can be achieved by adjusting the PDA/MMT mass ratio. Next, the dye adsorption performance was systematically explored. The resultant adsorbent (PM3) had a maximum adsorption capacity of 112.45 mg/g and its adsorption data was best described by a Langmuir isotherm and pseudo-second-order kinetic. Finally, the adsorption mechanisms and potential commercial practicability of the adsorbent were studied. Altogether, the designed adsorbent could effectively avoid generating the secondary pollution typical of adsorbents and it displayed excellent mechanical strength, thus opening up a new horizon in mitigating environmental pollution from textile industries.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123359DOI Listing
January 2021

Construction of functional curdlan hydrogels with bio-inspired polydopamine for synergistic periodontal antibacterial therapeutics.

Carbohydr Polym 2020 Oct 12;245:116585. Epub 2020 Jun 12.

School & Hospital of Stomatology, Wenzhou Medical University, Xueyuan West Road, Lucheng District, Wenzhou, 325027, China. Electronic address:

Curdlan, a bacteria-derived polysaccharide resource, possesses substantial potential for periodontal antimicrobial delivery. Here, the facile engineering of a functionalized curdlan/polydopamine (PDA) composite hydrogels was reported. The physiochemical evaluations of composite hydrogels proved their tunable properties associated with concentration of PDA including pore size, rheological property and swelling behavior. We have systematically assessed biocompatibility in vitro and found these hydrogels toxicity-free. Moreover, photothermal performance upon near infrared light (NIR) exposure was conducted and eventually indicated the best matches for antibacterial application. The acetate chlorhexidine (CHX) was chosen as a model antimicrobial and the release profiles demonstrated the entrapped CHX could be triggered and nicely controlled by NIR. The optimized bacteriostatic rate reached 99.9 %. Overall, we aimed to provide new curdlan-based hydrogels for periodontal antibacterial treatment by combining photothermal effect and antimicrobial simultaneously.
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http://dx.doi.org/10.1016/j.carbpol.2020.116585DOI Listing
October 2020

Development of a Novel, Multi-Parametric, MRI-Based Radiomic Nomogram for Differentiating Between Clinically Significant and Insignificant Prostate Cancer.

Front Oncol 2020 30;10:888. Epub 2020 Jun 30.

The First Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, China.

To develop and validate a predictive model for discriminating clinically significant prostate cancer (csPCa) from clinically insignificant prostate cancer (ciPCa). This retrospective study was performed with 159 consecutively enrolled pathologically confirmed PCa patients from two medical centers. The dataset was allocated to a training group ( = 54) and an internal validation group ( = 22) from one center along with an external independent validation group ( = 83) from another center. A total of 1,188 radiomic features were extracted from T2WI, diffusion-weighted imaging (DWI), and apparent diffusion coefficient (ADC) images derived from DWI for each patient. Multivariable logistic regression analysis was performed to develop the model, incorporating the radiomic signature, ADC value, and independent clinical risk factors. This was presented using a radiomic nomogram. The receiver operating characteristic (ROC) curve was utilized to assess the predictive efficacy of the radiomic nomogram in both the training and validation groups. The decision curve analysis was used to evaluate which model achieved the most net benefit. The radiomic signature, which was made up of 10 selected features, was significantly associated with csPCa ( < 0.001 for both training and internal validation groups). The area under the curve (AUC) values of discriminating csPCa for the radiomics signature were 0.95 (training group), 0.86 (internal validation group), and 0.81 (external validation group). Multivariate logistic analysis identified the radiomic signature and ADC value as independent parameters of predicting csPCa. Then, the combination nomogram incorporating the radiomic signature and ADC value demonstrated a favorable classification capability with the AUC of 0.95 (training group), 0.93 (internal validation group), and 0.84 (external validation group). Appreciable clinical utility of this model was illustrated using the decision curve analysis for the nomogram. The nomogram, incorporating radiomic signature and ADC value, provided an individualized, potential approach for discriminating csPCa from ciPCa.
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http://dx.doi.org/10.3389/fonc.2020.00888DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7339043PMC
June 2020

Solid-Vapor Interface Engineered Covalent Organic Framework Membranes for Molecular Separation.

J Am Chem Soc 2020 08 21;142(31):13450-13458. Epub 2020 Jul 21.

Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P. R. China.

Covalent organic frameworks (COFs) with intrinsic, tunable, and uniform pores are potent building blocks for separation membranes, yet poor processing ability and long processing time remain grand challenges. Herein, we report an engineered solid-vapor interface to fabricate a highly crystalline two-dimensional COF membrane with a thickness of 120 nm in 9 h, which is 8 times faster than that in the reported literature. Due to the ultrathin nature and ordered pores, the membrane exhibited an ultrahigh permeance (water, ∼411 L m h bar and acetonitrile, ∼583 L m h bar) and excellent rejection of dye molecules larger than 1.4 nm (>98%). The membrane exhibited long-term operation which confirmed its outstanding stability. Our solid-vapor interfacial polymerization method may evolve into a generic platform to fabricate COFs and other organic framework membranes.
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http://dx.doi.org/10.1021/jacs.0c04589DOI Listing
August 2020
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