Publications by authors named "Jianli Liu"

109 Publications

Toxic response of the freshwater green algae Chlorella pyrenoidosa to combined effect of flotation reagent butyl xanthate and nickel.

Environ Pollut 2021 May 3;286:117285. Epub 2021 May 3.

School of Water Resources and Environment, Research Center of Environmental Science and Engineering, China University of Geosciences (Beijing), 29 Xueyuan Road, Haidian District, 100083, Beijing, China.

Butyl Xanthate (BX) is a typical flotation reagent used to extract non-ferrous nickel ores, discharged into the surrounding environment of mining areas in large quantities. However, few studies have focused on the toxicity of combined pollution of BX and nickel (Ni) on aquatic plants, especially phytoplankton, the main producer of aquatic ecosystems. The toxicity and potential mechanism of single and combined pollution of BX and Ni at different concentrations (0-20 mg L) on typical freshwater algae (Chlorella pyrenoidosa) were studied. BX slightly stimulated the growth of C. pyrenoidosa on the first day, but Ni and Ni/BX mixture significantly inhibited it during incubation. Results showed that the inhibition rate (I) of the pollutants on the growth of C. pyrenoidosa followed the order: Ni/BX mixture > Ni > BX. The 96-h 20% effective inhibitory concentrations (96h-EC) of Ni and BX on C. pyrenoidosa growth were 3.86 mg L and 19.25 mg L, respectively, indicating C. pyrenoidosa was sensitive to pollutants. The content of total soluble protein (TSP) and chlorophyll a (Chl-a) changed significantly, which may be caused by the damage of pollutants to cell structures (cell membranes and chloroplasts). In addition, the I of pollutants on C. pyrenoidosa growth was related to dose, superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA). The increasement of reactive oxygen species (ROS), antioxidant enzymes (SOD and CAT), and MDA content, suggested C. pyrenoidosa suffered from oxidative stress, leading to lipid oxidation. These results will help to understand the toxicity mechanism of pollutants in typical mining areas and assess the environmental risks of pollutants to primary producers in aquatic ecosystems.
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http://dx.doi.org/10.1016/j.envpol.2021.117285DOI Listing
May 2021

A model for phenotyping crop fractional vegetation cover using imagery from unmanned aerial vehicles.

J Exp Bot 2021 May 8. Epub 2021 May 8.

College of Biosystems Engineering and Food Science, and State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou, P.R. China.

Fractional vegetation cover (FVC) is the key trait of interest for characterizing crop growth status in crop breeding and precision management. Accurate quantification of FVC among different breeding lines, cultivars, and growth environments is a challenging task, especially because of the large spatiotemporal variability in complex field conditions. This study presents an ensemble modeling strategy for phenotyping crop FVC from unmanned aerial vehicle (UAV)-based multispectral images by coupling PROSAIL with gap probability model (PROSAIL-GP). Seven field experiments for four main crops were conducted, and canopy images were acquired using a UAV platform equipped with RGB and multispectral cameras. The PROSAIL-GP model successfully retrieved FVC in oilseed rape (Brassica napus L.) with coefficient of determination, root mean square error (RMSE), and relative RMSE (rRMSE) of 0.79, 0.09, and 18%, respectively. The robustness of the proposed method was further examined with rice (Oryza sativa L.), wheat (Triticum aestivum L.), and cotton (Gossypium hirsutum L.), and the high accuracy of FVC retrieval was obtained with rRMSE of 12%, 6%, and 6%, respectively. The findings suggest that the proposed method can efficiently retrieve crop FVC from UAV images at a high spatiotemporal domain, which would be a promising tool for precision crop breeding.
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http://dx.doi.org/10.1093/jxb/erab194DOI Listing
May 2021

A novel gene recombinant collagen hemostatic sponge with excellent biocompatibility and hemostatic effect.

Int J Biol Macromol 2021 May 23;178:296-305. Epub 2021 Feb 23.

Medical Research and Development Center, Shaanxi Huikang Bio-Tech Co., Ltd., Xi'an 710054, China. Electronic address:

Effect and biosafety are the most noteworthy aspect of the hemostatic materials for trauma treatment. In this work, we evaluated the biocompatibility and hemostatic effect of a novel recombinant collagen hemostatic sponge according to ISO 10993. In addition, the interaction between the recombinant collagen hemostatic sponge and blood cells was observed by scanning electron microscopy, moreover, the hemostatic effect was evaluated by blood clotting assay in vitro and liver hemorrhage models in vivo. As the results, the novel recombinant collagen hemostatic sponge enables to be biodegradable completely in vivo, without stimulation, sensitization, acute toxicity, hematolysis or obvious immune rejection. The procoagulant effect of recombinant hemostatic sponge in vitro is significantly more excellent than that of natural collagen sponge due to the more promotive capacity of blood cell adhesion. Meanwhile, the liver hemorrhage models showed that the hemostatic time of recombinant collagen sponge was 19.33 ± 4.64 s, which was significantly better than that of natural collagen sponge (hemostatic time 31.62 ± 5.63 s). Therefore, the novel recombinant collagen hemostatic sponge with satisfactory biocompatibility and significant hemostatic effect can be performed as a potential novel type of clinical hemostatic products for research and development.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.02.162DOI Listing
May 2021

An Environmental Dilemma for China During the COVID-19 Pandemic: The Explosion of Disposable Plastic Wastes.

Bull Environ Contam Toxicol 2021 Feb 29;106(2):237-240. Epub 2021 Jan 29.

School of Environmental and Civil Engineering, Jiangnan University, Wuxi, 214122, China.

Plastic pollution control has been on top of the political agenda in China. In January 2020, China announced a phased ban on the production and usage of various types of single-use plastics as a solution to environmental pollution problems. However, the outbreak of COVID-19 seems to be a new obstacle to the ban on single-use plastic products. To basically satisfied the daily necessities and contain the spread of SARS-CoV-2 under the background of the regular epidemic prevention and control in China, online ordering, contactless delivery and wearing mask have become an important and feasible way of daily life. However, the unrestrained use of disposable plastic bags, lunch boxes and masks within the nationwide quarantine leads to hundreds of millions of plastics wastes every day. The potential environmental pollution caused by the use of disposable plastic products during the pandemic should arouse social concern. The Chinese government should manage environmental protection in parallel with anti-pandemic endeavors as the situation of the pandemic evolves.
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http://dx.doi.org/10.1007/s00128-021-03121-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7845788PMC
February 2021

The spatial transmission of SARS-CoV-2 in China under the prevention and control measures at the early outbreak.

Arch Public Health 2021 Jan 13;79(1). Epub 2021 Jan 13.

The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.

Background: Since severe acute respiratory syndrome coronavirus, 2 (SARS-CoV-2) was firstly reported in Wuhan City, China in December 2019, Novel Coronavirus Disease 2019 (COVID-19) that is caused by SARS-CoV-2 is predominantly spread from person-to-person on worldwide scales. Now, COVID-19 is a non-traditional and major public health issue the world is facing, and the outbreak is a global pandemic. The strict prevention and control measures have mitigated the spread of SARS-CoV-2 and shown positive changes with important progress in China. But prevention and control tasks remain arduous for the world. The objective of this study is to discuss the difference of spatial transmission characteristics of COVID-19 in China at the early outbreak stage with resolute efforts. Simultaneously, the COVID-19 trend of China at the early time was described from the statistical perspective using a mathematical model to evaluate the effectiveness of the prevention and control measures.

Methods: In this study, the accumulated number of confirmed cases publicly reported by the National Health Committee of the People's Republic of China (CNHC) from January 20 to February 11, 2020, were grouped into three partly overlapping regions: Chinese mainland including Hubei province, Hubei province alone, and the other 30 provincial-level regions on Chinese mainland excluding Hubei province, respectively. A generalized-growth model (GGM) was used to estimate the basic reproduction number to evaluate the transmissibility in different spatial locations. The prevention and control of COVID-19 in the early stage were analyzed based on the number of new cases of confirmed infections daily reported.

Results: Results indicated that the accumulated number of confirmed cases reported from January 20 to February 11, 2020, is well described by the GGM model with a larger correlation coefficient than 0.99. When the accumulated number of confirmed cases is well fitted by an exponential function, the basic reproduction number of COVID-19 of the 31 provincial-level regions on the Chinese mainland, Hubei province, and the other 30 provincial-level regions on the Chinese mainland excluding Hubei province, is 2.68, 6.46 and 2.18, respectively. The consecutive decline of the new confirmed cases indicated that the prevention and control measures taken by the Chinese government have contained the spread of SARS-CoV-2 in a short period.

Conclusions: The estimated basic reproduction number thorough GGM model can reflect the spatial difference of SARS-CoV-2 transmission in China at the early stage. The strict prevention and control measures of SARS-CoV-2 taken at the early outbreak can effectively reduce the new confirmed cases outside Hubei and have mitigated the spread and yielded positive results since February 2, 2020. The research results indicated that the outbreak of COVID-19 in China was sustaining localized at the early outbreak stage and has been gradually curbed by China's resolute efforts.
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http://dx.doi.org/10.1186/s13690-021-00529-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804902PMC
January 2021

Cadmium induced BEAS-2B cells apoptosis and mitochondria damage via MAPK signaling pathway.

Chemosphere 2021 Jan 17;263:128346. Epub 2020 Sep 17.

Department of Biological Sciences, School of Life Science, Liaoning University, Chongshan Road 66, Shenyang, 110036, PR China. Electronic address:

Cadmium, a heavy metal pollutant in industrial production, is found in air, water and soil, which is harmful to human health and can lead to diseases, such as asthma, lung cancer, and emphysema. In this study, the toxicity of cadmium on human bronchial epithelial cells (BEAS-2B) was investigated. Cell viability, mitochondrial membrane potential, reactive oxygen species (ROS) level, apoptosis and the related signaling pathways were detected with MTT assay, Rhodamine staining, DCFH-DA staining, Hoechst33258 staining and Western blot methods respectively. The results showed that the cell viability decreased, the mitochondrial membrane potential declined, ROS was accumulated and apoptotic rate raised in BEAS-2B cells. Meanwhile, the expression of B-cell lymphoma-2 (Bcl-2) was downregulated, while the expression of Bcl-2-associated X protein (Bax) and the cleaved caspase-3 was upregulated, which indicated mitochondria-mediated intrinsic apoptosis pathway was activated. Furthermore, the phosphorylation of JNK, ERK and p38 was enhanced respectively, which manifested that MAPK signaling pathways were activated. Therefore, it could be concluded that cadmium could increase intracellular ROS, result in cellular oxidative stress, activate JNK, ERK and p38 MAPK pathways and ultimately lead to apoptosis of BEAS-2B cells by activating mitochondria-mediated intrinsic apoptosis pathway. This study provided useful information to elucidate the toxicity of cadmium and revealed the possible mechanism for the occurrence of lung disease induced by cadmium.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128346DOI Listing
January 2021

Inhibitory mechanism of sinensetin on α-glucosidase and non-enzymatic glycation: Insights from spectroscopy and molecular docking analyses.

Int J Biol Macromol 2021 Jan 26;166:259-267. Epub 2020 Oct 26.

Department of Biological Sciences, School of Life Science, Liaoning University, Shenyang 110036, PR China. Electronic address:

Inhibition of α-glucosidase and non-enzymatic glycation is regarded as an effective method to prevent and treat type 2 diabetes and its complications. In this study, the inhibition of sinensetin on α-glucosidase and non-enzymatic glycation was studied with multi-spectroscopic techniques and molecular docking analysis. The results of fluorescence spectroscopy analysis indicated that sinensetin quenched the endogenous fluorescence of α-glucosidase in static manner. The binding of sinensetin with α-glucosidase was a spontaneous process primarily driven by hydrophobic interaction. At 298 K, the binding constant was (5.70 ± 0.12) × 10 L·mol and the binding site number was 1. The conformation of α-glucosidase was altered by sinensetin, which was revealed by circular dichroism (CD), FTIR spectra, synchronous fluorescence and three-dimensional (3D) fluorescence spectroscopy methods. Molecular docking analysis demonstrated that sinensetin interacted with the amino acid residues of α-glucosidase, which might prevent the entrance of substrate, leading to the decrease of catalytic efficiency of α-glucosidase. Furthermore, glycation assays showed that sinensetin stabilized the structure of bovine serum albumins (BSA), interacted with BSA, strongly inhibited the formation of dityrosine, N'-formylkynurenine and advanced glycation end products (AGEs). This study provided useful information concerning sinensetin preventing and treating type 2 diabetes and its related complications.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.10.174DOI Listing
January 2021

The Identification of Anthocyanins from Padus racemosa and Its Protective Effects on H O -Induced INS-1 Cells Damage and STZ-Induced Diabetes Mice.

Chem Biodivers 2020 Nov 6;17(11):e2000382. Epub 2020 Oct 6.

School of Life Science, Liaoning University, Shenyang, 110036, P. R. China.

Oxidative damage in cells induced by reactive oxygen species (ROS) is a main factor in diabetes mellitus diseases progression. The composition of anthocyanins from Padus racemosa (APR) and the protective effects of APR on H O -induced rat insulinoma (INS-1) cells damage and streptozocin (STZ)-induced diabetes mice were investigated in this study. The main components of APR were cyanidin-cyanidin glucosyl-rutinoside, cyanidin-cyanidin xylosyl-rutinoside, cyanidin-xylosyl-glucoside and cyanidin-rutinoside, which were determined by liquid chromatography-mass spectrometry (LC/MS). APR could scavenge the 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and superoxide radical in vitro. ROS level was decreased and the cell viability was increased in INS-1 cells after treated with APR. Cell apoptosis induced by H O in INS-1 cells was decreased after incubation with APR. APR could decrease the phosphorylation of p38 and the nuclear translocation of p65, which indicated that APR could inhibit the activation of p38 Mitogen-activated protein kinase (MAPK) and Nuclear factor kappa B (NF-κB) cell signaling pathways. Meanwhile, APR could effectively reduce the blood glucose and blood lipid in STZ-induced diabetic mice. These results suggested that APR might be a potential agent for diabetes mellitus diseases treatment.
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http://dx.doi.org/10.1002/cbdv.202000382DOI Listing
November 2020

Inhibitory effects of Lentinus edodes mycelia polysaccharide on α-glucosidase, glycation activity and high glucose-induced cell damage.

Carbohydr Polym 2020 Oct 17;246:116659. Epub 2020 Jun 17.

Department of Biological Sciences, School of Life Science, Liaoning University, No. 66 Chongshan Road, Shenyang 110036, Liaoning Province, PR China. Electronic address:

At present, diabetes and diabetic complications have become one of the serious diseases affecting human health. In this study, the inhibitory effects of Lentinus edodes mycelia polysaccharide (LMP) on α-glucosidase activity, the formation of advanced glycation end products (AGEs) and high glucose-induced human umbilical vein endothelial cells (HUVECs) damage were explored. The interaction between LMP and α-glucosidase and the inhibition against AGEs formation were investigated with spectroscopic techniques. The results revealed that LMP had a reversible inhibition on α-glucosidase activity in a mixed-type manner. When the concentration of LMP was 2.7 mM, the inhibition rate was 34.38 %. LMP quenched the fluorescence of α-glucosidase through the static quenching and formed the LMP-α-glucosidase complex. At 310 K, the number of binding sites (n) and binding constant (K) were 1.01 and 3.71 × 10 L mol, respectively. In addition, LMP could inhibit the formation of AGEs. Compared with 40 mM glucose treatment group, treatment with 0.05 mM LMP for 48 h increased the cell viability from 70.17% to 91.14% and decreased ROS production from 3.33-fold to 1.21-fold. LMP inhibited high glucose-induced activation of MAPK signaling pathways. These findings may promote the application of LMP in the functional food industry.
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http://dx.doi.org/10.1016/j.carbpol.2020.116659DOI Listing
October 2020

Nucleosome movement analysis based on second-order information entropy and density functional theory.

Biophys Chem 2020 10 21;265:106436. Epub 2020 Jul 21.

School of Water Resource and Environment Engineering, China University of Geosciences (Beijing), Xueyuan Road NO.29th, Beijing 100083, China. Electronic address:

Dynamics of +1 and -1 nucleosomes near TSS of yeast chromosome 2 were analyzed by using second-order information entropy and density functional theory method. Second-order information entropy can measure the interaction intensity between nucleosome sequences and nucleosome histones based on the intensity of base association. In addition, density functional theory method can be used to obtain the global interaction intensity between nucleosome sequences and nucleosome histones based on energy state size and active or non-active state of binucleoside pairs. Our results showed asymmetry of interaction intensity on both sides of the nucleosome central site, and that +1 nucleosomes tend to move toward the 5'-end and -1 nucleosomes tend to move toward the 3'-end. Under the dynamic balance of nucleosome movement, in roder to shut down gene transcription, +1 and -1 nucleosomes will cover TSS. If the dynamic balance is destroyed, +1 and -1 nucleosomes stay away from each other to expose TSS to restart gene transcription. The movement trend of +1 and -1 nucleosomes coincides with the biological mechanism of gene transcription and non-transcription, and the nucleosome sequences contain the dynamic information of nucleosome movement, which provides effective technical support for the study of gene transcription regulation mechanism.
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http://dx.doi.org/10.1016/j.bpc.2020.106436DOI Listing
October 2020

Internally controlled recombinase-aided amplification (IC-RAA) assays for the detection of human papillomavirus genotypes 16 and 18 using extracted DNA and samples treated with nucleic acid releasing agent.

Arch Virol 2020 Oct 17;165(10):2241-2247. Epub 2020 Jul 17.

NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155, Changbai Street, Changping District, Beijing, 102206, China.

Cervical cancer is primarily caused by persistent infection with high-risk human papillomavirus (HPV), and 70% of cases are associated with HPV16 and 18 infections. The objective of this study was to establish rapid, simple, and sensitive internally controlled recombinase-aided amplification (IC-RAA) assays for the detection of HPV16 and 18. The assays were performed at 39 ℃ and were completed within 30 min. A total of 277 clinical samples of exfoliated cervical cells were tested by IC-RAA assays and commercial HPV real-time fluorescent PCR kits using extracted DNA and samples treated with nucleic acid releasing agent. The analytical sensitivity of the IC-RAA assay was found to be 10 copies/μL for the detection of HPV16 and 18 when using recombinant plasmids as targets. The optimal concentration of the internal control (IC) plasmid and 18 was 1000 copies/μL for HPV16 and 100 copies/μL for HPV18. The clinical sensitivity of the IC-RAA assays for HPV16 using extracted DNA and samples treated with nucleic acid releasing agent was 98.73% and 97.47%, respectively, with kappa values of 0.977 (P < 0.01) and 0.955 (P < 0.01), respectively, and 100% The specificity in both cases. For HPV18, the sensitivity and specificity were 100%, and the kappa value was 1 for both samples (P < 0.01). The IC-RAA assay is a promising tool for the detection of HPV16 and HPV18, especially in resource-constrained settings.
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http://dx.doi.org/10.1007/s00705-020-04722-3DOI Listing
October 2020

Engineering Biomolecular Self-Assembly at Solid-Liquid Interfaces.

Adv Mater 2021 Jun 6;33(23):e1905784. Epub 2020 Jul 6.

Department of Materials Science and Engineering, University of Washington, Seattle, WA, 98105, USA.

Biomolecular self-assembly is a key process used by life to build functional materials from the "bottom up." In the last few decades, bioengineering and bionanotechnology have borrowed this strategy to design and synthesize numerous biomolecular and hybrid materials with diverse architectures and properties. However, engineering biomolecular self-assembly at solid-liquid interfaces into predesigned architectures lags the progress made in bulk solution both in practice and theory. Here, recent achievements in programming self-assembly of peptides, proteins, and peptoids at solid-liquid interfaces are summarized and corresponding applications are described. Recent advances in the physical understandings of self-assembly pathways obtained using in situ atomic force microscopy are also discussed. These advances will lead to novel strategies for designing biomaterials organized at and interfaced with inorganic surfaces.
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http://dx.doi.org/10.1002/adma.201905784DOI Listing
June 2021

The Lessons and Experiences That Can Be Learned From China in Fighting Coronavirus Disease 2019.

Front Public Health 2020 27;8:227. Epub 2020 May 27.

Jiangsu Province Hospital and Nanjing Medical University First Affiliated Hospital, Nanjing, China.

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http://dx.doi.org/10.3389/fpubh.2020.00227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266930PMC
January 2021

A study on the protective effects of taxifolin on human umbilical vein endothelial cells and THP-1 cells damaged by hexavalent chromium: a probable mechanism for preventing cardiovascular disease induced by heavy metals.

Food Funct 2020 May 22;11(5):3851-3859. Epub 2020 Apr 22.

Department of Biological Sciences, School of Life Science, Liaoning University, No. 66 Chongshan Road, Shenyang 110036, Liaoning Province, P R China.

Hexavalent chromium [Cr(vi)] which is a kind of heavy metal with strong oxidizing ability can induce cardiovascular disease (CVD), while taxifolin can protect cells and organisms against suffering from oxidative stress. In this study, the inhibitory effects of taxifolin against Cr(vi)-induced cell damage in human umbilical vein endothelial cells (HUVECs) and THP-1 cells were investigated. Cr(vi) could increase the phosphorylation of p38 and JNK, regulate the expression of Bax and Bcl-2 in both cell lines. Meanwhile, the Cr(vi) stimulation led to an increase of the expression of ICAM-1 and VCAM-1, and upregulated the adhesion of THP-1 cells to HUVECs. Furthermore, Cr(vi) could induce the activation of the nuclear factor kappa B (NF-κB) signaling pathway, the accumulation of p65 in the nucleus, and the increase in the phosphorylation of IκB and the expression of cleaved caspase-1 and IL-1β in THP-1 cells. However, taxifolin could reverse the effects by inhibiting the activation of mitogen-activated protein kinases (MAPKs) and NF-κB signaling pathways, regulating the expression of apoptosis-related proteins, and alleviating the adhesion of THP-1 cells to HUVECs. Our findings demonstrated that taxifolin was a potential agent to prevent endothelial dysfunction, monocyte inflammation and cell adhesion induced by Cr(vi).
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http://dx.doi.org/10.1039/d0fo00567cDOI Listing
May 2020

Quantitative structure-chromatographic retention relationship of synthesized peptides (HGRFG, NPNPT) and their derivatives.

Anal Biochem 2020 05 27;597:113653. Epub 2020 Feb 27.

College of Life Science, Northwest University, Xi'an, 710069, PR China. Electronic address:

Carapax Trionycis extract peptides (HGRFG, NPNPT) are able to protect against CCl-induced liver fibrosis. Therefore, this study applies to deal with chromatographic lipophilicity determination of synthesized peptides (HGRFG, NPNPT) and their derivatives using reversed-phase high performance liquid chromatography (RP-HPLC) combined with methanol-water mobile phase and two reversed-phase chromatographic columns (COSMOISL 5C18-MS-II and SHIMADZU-C18). The chromatographic lipophilicity of the analyzed compounds was expressed as logk constant and correlated with lipophilicity descriptors. Quantitative structure-retention relationships (QSRR) analysis was performed to imitate chromatographic lipophilicity behavior using molecular descriptors. Modeling was performed using linear regression (LR) and multiple linear regression (MLR) methods with the help of principal component analysis (PCA) and hierarchical cluster analysis (HCA). The most influential molecular descriptors were lipophilicity descriptors, which are important for molecules ability to pass through biological membranes. All established QSRR models were statistically validated by standards, cross- and external validation parameters. According to these statistical validation parameters, MLR models (R > 0.856) were better for chromatographic lipophilicity prediction of peptide compounds. It can be concluded that chromatographic systems with COSMOISL 5C18-MS-II column were better for modeling of logk than systems with SHIMADZU-C18 column. Modeling was performed in order to obtain lipophilicity profiles of investigated compounds as future drug candidates.
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http://dx.doi.org/10.1016/j.ab.2020.113653DOI Listing
May 2020

Assessment of biological properties of recombinant collagen-hyaluronic acid composite scaffolds.

Int J Biol Macromol 2020 Apr 5;149:1275-1284. Epub 2020 Feb 5.

Medical Research and Development Center, Shaanxi Huikang Bio-Tech Co.,Ltd, Xi'an 710054, China. Electronic address:

Objective: Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds were prepared to thoroughly investigate their biological properties.

Methods: The rCOL and HA composite scaffolds were formulated via lyophilization. The scaffolds were characterized for various materials properties, including porosity, surface modification, and degradation rates. Biological properties such as in vitro cytotoxicity, cell adhesion, proliferation and migration effects were also evaluated.

Results: The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied. Scanning electron microscopy (SEM) revealed that the composites formed a three-dimensional network structure with uniform pore distribution. The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).

Conclusion: The rCOL-HA composite prepared via lyophilization after cross-linking is characterized by high porosity, high water absorption, and good interaction between the material and cells, as well as good biodegradability. Compared with rCOL materials, rCOL-HA has increased mechanical strength, water absorption and thermal stability. The biocompatibility and fibroblast proliferation of rCOL-HA have excellent biological performance, providing a new material for wound healing applications.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.02.023DOI Listing
April 2020

Resveratrol protects human bronchial epithelial cells against nickel-induced toxicity via suppressing p38 MAPK, NF-κB signaling, and NLRP3 inflammasome activation.

Environ Toxicol 2020 May 13;35(5):609-618. Epub 2020 Jan 13.

School of Life Science, Liaoning University, Shenyang, China.

Nickel is a common environmental pollutant that can impair the lung, but the underlying mechanisms have not yet been fully elucidated. Furthermore, natural products are generally used to inhibit cell damage induced by heavy metal. Resveratrol possesses wide biological activities, including anti-inflammation and antioxidative stress. This study was conducted to explore the toxicity of nickel on human bronchial epithelial (BEAS-2B) cells and evaluate the protective effect of resveratrol. The results showed that nickel could induce cell apoptosis, increase oxidative stress, and promote the expression of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-1β, IL-6, IL-8, C-reaction protein. Western blot analysis showed that nickel activated p38 mitogen-activated protein kinase (MAPK), nuclear factor-kappa B, and nucleotide-binding oligomerization domain-like receptor pyrin-domain-containing protein 3 pathways, while resveratrol could reverse these effects. Our results suggested that resveratrol could protect BEAS-2B cells from nickel-induced cytotoxicity. Therefore, resveratrol is a potential chemopreventive agent against nickel-induced lung disease.
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http://dx.doi.org/10.1002/tox.22896DOI Listing
May 2020

Protective Effects of Anthocyanins from Coreopsis tinctoria against Oxidative Stress Induced by Hydrogen Peroxide in MIN6 Cells.

Chem Biodivers 2020 Feb 22;17(2):e1900587. Epub 2020 Jan 22.

School of Life Sciences, Liaoning University, Shenyang, 110036, P. R. China.

Anthocyanins (AC) from Coreopsis tinctoria possesses strong antioxidant properties, while the effects of AC on cells damage induced by reactive oxygen species (ROS) in diabetes mellitus diseases progression have not been reported. The present study was carried out to evaluate the protective property of AC against cellular oxidative stress with an experimental model, H O -exposed MIN6 cells. AC could reverse the decrease of cell viability induced by H O and efficiently suppressed cellular ROS production and cell apoptosis. In addition, Real-time PCR and Western blot analyses indicated that AC could protect MIN6 cells against oxidative injury through increasing the translocation of Nrf2 into nuclear, decreasing the phosphorylation level of p38 and up-regulating the protein expression of antioxidant enzyme (SOD1, SOD2 and CAT). Thus, this study provides evidence to support the beneficial effect of AC in inhibiting MIN6 cells from H O -induced oxidative injury.
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http://dx.doi.org/10.1002/cbdv.201900587DOI Listing
February 2020

Potentially Critical Roles of , , in the Progression of Septic Cardiomyopathy Through Integrated Bioinformatics Analysis.

DNA Cell Biol 2020 Jan 27;39(1):105-117. Epub 2019 Nov 27.

Department of Cardiology of Affiliated Hospital, Weifang Medical University, Weifang, China.

Septic cardiomyopathy (SC) is a rare and harmful cardiovascular disease with decreased left ventricular (LV) output and multiple organ failure, which poses a serious threat to human life. Despite the advances in SC, its diagnostic basis and treatment methods are limited, and the specific diagnostic biomarkers and its candidate regulatory targets have not yet been fully established. In this study, the GSE79962 gene expression profile was retrieved, with 20 patients with SC and 11 healthy donors as control. Weighted gene coexpression network analysis (WGCNA) was employed to investigate gene modules that were strongly correlated with clinical phenotypes. Blue module was found to be most significantly related to SC. Moreover, Gene Ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed on the coexpression genes in blue module and showed that it was associated with metabolic pathways, oxidative phosphorylation, and cardiac muscle contraction. Furthermore, a total of 10 hub genes , , , , (mitochondrial ribosomal protein L16), , , , , and (pyruvate dehydrogenase E1 beta subunit) in the blue module were identified at transcriptional level and further validated at translational level in myocardium of an lipopolysaccharide-induced septic cardiac dysfunction mouse model. Overall, the results of quantitative real-time polymerase chain reaction were consistent with most of the microarray analysis results. Intriguingly, we observed that the highest change was , , and . These identified and validated genes provided references that would advance the understanding of molecular mechanisms of SC. Taken together, using WGCNA, the hub genes , , and might serve as potential biomarkers for diagnosis and/or therapeutic targets for precise treatment of SC in the future.
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http://dx.doi.org/10.1089/dna.2019.4859DOI Listing
January 2020

Pectin oligosaccharides from hawthorn (Crataegus pinnatifida Bunge. Var. major) inhibit the formation of advanced glycation end products in infant formula milk powder.

Food Funct 2019 Dec;10(12):8081-8093

Department of Food Science, College of Light Industry, Liaoning University, Liaoning Engineering Research Center for Food Bioprocessing, Shenyang Key Laboratory of Food Bioprocessing and Quality Control, Shenyang 110036, China.

Pectin oligosaccharides (POSs) can not only be used as prebiotics to promote the growth of beneficial bacteria in the intestine but also can be used as natural food-borne antiglycation agents to inhibit the formation of advanced glycation end products (AGEs) in vitro, which is related to their structure, including molecular weight and galacturonic acid content. In this study, haw polysaccharides (HPSs) were isolated and purified, and POSs with high antiglycation activity in vitro were prepared. On this basis, the inhibitory effect of POSs on the formation of AGEs in infant formula milk powder was investigated. The results showed that no obvious inhibitory effect of POSs was found on the formation of AGEs in infant formula milk powder under accelerated storage at 25 °C and 45 °C. But, POSs showed a strong inhibitory effect on the formation of furosine, Nε-carboxymethyllysine (CML), Nε-carboxyethyllysine (CEL) and the total AGEs in infant formula milk powder under accelerated storage at 65 °C. In addition, POSs also had a strong inhibitory effect on the formation of lipid oxidation products but did not affect the formation of protein degradation products. Simultaneously, the cytotoxicity experiments using human umbilical vein endothelial cells showed that the infant formula milk powder supplemented with POSs had the lowest cytotoxicity compared to the blank control (BC) and GOS/FOS supplementation milk powder under accelerated storage at 65 °C, which may be related to the inhibitory effect of POSs on the formation of AGEs in infant formula milk powder. Furthermore, the in vitro fermentation experiments showed that the antiglycation process did not affect the prebiotic activity of POSs in infant formula milk powder.
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http://dx.doi.org/10.1039/c9fo01041fDOI Listing
December 2019

Eriodictyol and naringenin inhibit the formation of AGEs: An in vitro and molecular interaction study.

J Mol Recognit 2020 01 21;33(1):e2814. Epub 2019 Oct 21.

School of Life Science, Liaoning University, Shenyang, PR China.

Maillard reaction occurs between the carbonyl group of reducing sugars and the free amino groups of protein, which eventually results in the formation and accumulation of advanced glycation end products (AGEs) irreversibly. Excessive production of AGEs is associated with many diseases, such as Alzheimer disease, neuropathy, retinopathy, and nephropathy. In this study, the effects of eriodictyol and naringenin on the inhibition of AGEs were studied with bovine serum albumin (BSA)-methylglyoxal (MGO) model by spectroscopic techniques and molecular docking methods. The fluorescence spectroscopy results suggested that eriodictyol and naringenin could inhibit the formation of AGEs. Circular dichroism (CD) studies indicated that eriodictyol and naringenin could stabilize the structure of BSA and inhibit the formation of AGEs. The molecular docking results demonstrated that eriodictyol formed two hydrogen bonds with Lys 350 and Leu 480 and the main forces were hydrogen bonding and hydrophobic interactions. However, naringenin interacted with Arg 484 of BSA, and the main force was hydrophobic interaction. It can be concluded that eriodictyol and naringenin can inhibit the formation of AGEs and eriodictyol has stronger inhibitory activity of AGEs than that of naringenin, which is probably due to the additional hydroxyl group in the position C-3' of B ring of eriodictyol.
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http://dx.doi.org/10.1002/jmr.2814DOI Listing
January 2020

The biosynthesis of camptothecin derivatives by seedlings.

Nat Prod Res 2019 Oct 21:1-5. Epub 2019 Oct 21.

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University , Xi'an , China.

10-hydroxycamptothecin and 9-methoxycamptothecin, naturally occurring camptothecin derivatives, are reportedly present in with a powerful cytotoxic effect and strong antitumor activity. In this paper, we studied the derivatization reaction of camptothecin catalyzed by seedlings. HPLC traced the reaction between exogenous camptothecin and seedlings. The results showed that the exogenous camptothecin was converted into 10-hydroxycamptothecin and 9-methoxycamptothecin by the tender roots and stems of seedlings, which would be a new method for the synthesis of two camptothecin derivatives.
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http://dx.doi.org/10.1080/14786419.2019.1678616DOI Listing
October 2019

Distribution of camptothecin biosynthetic intermediates and identification the rate-limiting step of camptothecin biosynthesis.

Nat Prod Res 2019 Sep 19:1-8. Epub 2019 Sep 19.

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University , Xi'an , China.

Two key biosynthetic intermediates (pumiloside and strictosamide) of camptothecin were isolated. A high performance liquid chromatography-ultraviolet (HPLC-UV) method was developed to determine four main alkaloid compounds (pumiloside, strictosamide, camptothecin and 10-hydroxycamptothecin) and estimate two minor compounds (deoxypumiloside, 9-methoxycamptothecin) simultaneously in different parts of , with a good linearity and R > 0.999 for all curves. The results indicated that there was a positive correlation between the two key intermediates (strictosamide and pumiloside) and camptothecin in vivo. The speculation that the root was the synthetic position of camptothecin in vivo was confirmed. The rate-limiting step of camptothecin biosynthesis was estimated the step from pumiloside to deoxypumiloside based on its concentration fall sharply.
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http://dx.doi.org/10.1080/14786419.2019.1665252DOI Listing
September 2019

High expression of cytokeratin CAM5.2 in esophageal squamous cell carcinoma is associated with poor prognosis.

Medicine (Baltimore) 2019 Sep;98(37):e17104

Department of Pathology.

Esophageal cancer is a common human malignant tumor with high mortality. Glandular epithelial markers, such as CAM5.2, can be expressed in esophageal squamous cell carcinoma (ESCC), but the clinical significance of these cells in ESCC remains elusive.Immunohistochemical analysis of CAM5.2 was performed on 604 ESCC specimens using tissue microarray. Our study design and study population used retrospective cohorts based on the hospital information system and pathological information management system which included medical information, date of admission, procedures undergone, registration, examinations, and medication.In total, positive staining of CAM5.2 was 145 of 604 (24%). Statistical analysis showed that the expression of CAM5.2 had no relationship with sex, age, tumor differentiation, tumor size, tumor-node-metastasis (TNM) classification, and lymph node metastasis, but it was significantly associated with poor prognosis of overall survival (P = .0041) and disease-free survival (P = .0048) in ESCC patients.Herein, we report for the first time that the high expression of the CAM 5.2 is an independent predictor of poor prognosis in patients with ESCC.
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http://dx.doi.org/10.1097/MD.0000000000017104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6750307PMC
September 2019

Antitumor activity of alantolactone in lung cancer cell lines NCI-H1299 and Anip973.

J Food Biochem 2019 09 5;43(9):e12972. Epub 2019 Jul 5.

Department of Biological Sciences, School of Life Science, Liaoning University, Shenyang, P.R. China.

Alantolactone is a sesquiterpene lactone extracted from Inula helenium L. plants possessing many biological activities, including anti-inflammatory, antiproliferation, and antimicrobial. The inhibitory effects and the underlying mechanisms of alantolactone on lung cancer cells NCI-H1299 and Anip973 were investigated in this study. The results showed that alantolactone could decrease cell viability and induce cell apoptosis of NCI-H1299 and Anip973. After the cells were treated with alantolactone, the expression of Bcl-2 decreased, while the expression of Bax increased, the expression of MMP-9, MMP-7, and MMP-2 gradually decreased after alantolactone treatment. Furthermore, results showed that alantolactone could activate p38 MAPK pathway and suppress NF-κB pathway, which are involving in lung cancer development. These results indicated that alantolactone was a potential agent for lung cancer treatment. PRACTICAL APPLICATIONS: Lung cancer is one of the most common contributors of cancer death in the world. Chemoprevention and chemotherapy with natural substances are prospective methods for lung cancer treatment. In recent years, the anti-cancer activity of various sesquiterpene lactones has attracted a great deal of interest. Alantolactone is the major active sesquiterpene lactones isolated from Inula helenium L, which is used as a medicine in ancient Romans due to wide range of pharmacological activities. The results obtained from this study revealed the inhibitory effects of alantolactone on lung cancer cells and might provide some experimental basis for prevention and treatment of lung cancer with alantolactone.
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http://dx.doi.org/10.1111/jfbc.12972DOI Listing
September 2019

Improving the Performance of the Mini 2000 Mass Spectrometer with a Triboelectric Nanogenerator Electrospray Ionization Source.

ACS Omega 2018 Sep 28;3(9):12229-12234. Epub 2018 Sep 28.

Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, China.

Balancing the contradiction between portability and analytical performances of a miniaturized mass spectrometer is vital to extend its on-site applications. In this study, triboelectric nanogenerator (TENG)-driven ion sources were coupled with our home-built Mini 2000 system and applied to the analyses of different samples. Compared with the conventional direct current (DC) nanoelectrospray ionization (nanoESI) source, the ion intensity of the TENG-nanoESI miniature mass spectrometer was improved by ∼3 times. Moreover, maybe due to the different pathways of ion formation in comparison with DC electrospray, TENG electrospray is shown to reduce the salt suppression effect during ionization. With these figures of merit, the direct detection of reserpine in saliva was demonstrated using the TENG-Mini 2000 system.
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http://dx.doi.org/10.1021/acsomega.8b01777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6645661PMC
September 2018

Microfibers: a preliminary discussion on their definition and sources.

Environ Sci Pollut Res Int 2019 Oct 23;26(28):29497-29501. Epub 2019 Aug 23.

College of Textile and Clothing, Jiangnan University, Wuxi, 214122, China.

Microfibers are a major component of microplastics and have been found nearly everywhere, especially in marine and freshwater habitats around the world. Therefore, microfibers have gained considerable attention in environmental science research. However, there is still no clear consensus on a definition that can encompass all necessary properties to describe microfibers as emerging pollutants. Therefore, we propose a definition for debate by taking the related descriptions of microplastics and textile fibers as references. Moreover, the potential sources from the perspectives of textile engineering, including production, use, care, and end-of-life disposal of fibrous materials, are discussed. For further investigation of microfiber pollution, the gap between current knowledge and major microfiber pollution concerns must be bridged.
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http://dx.doi.org/10.1007/s11356-019-06265-wDOI Listing
October 2019

Caffeic Acid Inhibits the Formation of Advanced Glycation End Products (AGEs) and Mitigates the AGEs-Induced Oxidative Stress and Inflammation Reaction in Human Umbilical Vein Endothelial Cells (HUVECs).

Chem Biodivers 2019 Oct 3;16(10):e1900174. Epub 2019 Sep 3.

School of Life Sciences, Liaoning University, 66 Chongshan Middle Road, Shenyang, 110036, P. R. China.

The advanced glycation end products (AGEs) are the compounds produced by non-enzymatic glycation reaction of proteins and sugars, which can induce the generation of free radicals and the expression of inflammatory factors, thereby playing an important role in vascular dysfunction in diabetes. To investigate the effects of caffeic acid (CA) on glycation formed by glucose and protein, various spectroscopic techniques and molecular docking methods were carried out. Furthermore, the protective effects of CA on human umbilical vein endothelial cells (HUVECs) damaged by AGEs were detected. The results indicated that CA inhibited AGEs formation in vitro, decreased the expression of IL-1β, IL-18, ICAM-1, VCAM-1, NLRP3, Caspase-1 and CRP (C-reactive protein) and reduced the ROS in HUVECs exposed to AGEs. Our findings suggested that the supplementation with dietary CA could prevent and delay the AGEs-induced vascular dysfunction in diabetes.
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http://dx.doi.org/10.1002/cbdv.201900174DOI Listing
October 2019

Nonferrous metal (loid) s mediate bacterial diversity in an abandoned mine tailing impoundment.

Environ Sci Pollut Res Int 2019 Aug 25;26(24):24806-24818. Epub 2019 Jun 25.

School of Water Resource and Environment Engineering, Research Center of Environmental Sciences and Engineering, China University of Geosciences (Beijing), 29 Xueyuan Road, Haidian District, Beijing, 100083, China.

Migration and transformation of toxic metal (loid) s in tailing sites inevitably lead to ecological disturbances and serious threats to the surroundings. However, the horizontal and vertical distribution of bacterial diversity has not been determined in nonferrous metal (loid) tailing ponds, especially in Guangxi China, where the world's largest and potentially most toxic sources of metal (loid) s are located. Distribution of bacterial communities was stable at horizontal levels. At the surface (0-10 cm), the stability was most attributed to Bacillus and Enterococcus, while bacterial communities at the subsurface (50 cm) were mainly contributed by Nitrospira and Sulfuricella. Variable vertical distribution of bacterial communities has led to the occurrence of specific genera and specific predicted functions (such as transcription regulation factors). Sulfurifustis (a S-oxidizing and inorganic carbon fixing bacteria) genera were specific at the surface, whereas Streptococcus-related genera were found at the surface and subsurface, but were more abundant in the latter depth. Physical-chemical parameters, such as pH, TN, and metal (loid) (As, Cd, Pb, Cu, and Zn) concentrations were the main drivers of bacterial community abundance, diversity, composition, and metabolic functions. These results increase our understanding of the physical-chemical effects on the spatial distribution of bacterial communities and provide useful insight for the bioremediation and site management of nonferrous metal (loid) tailings.
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http://dx.doi.org/10.1007/s11356-019-05092-3DOI Listing
August 2019

A novel polysaccharide from mycelia protects MIN6 cells against high glucose-induced damage via the MAPKs and Nrf2 pathways.

Food Nutr Res 2019 6;63. Epub 2019 Jun 6.

School of life Science, Liaoning University, Shenyang, Liaoning, China.

Background: Diabetes mellitus is one of the most widespread diseases in the world, high glucose can damage islet cells, it is important to discover new natural products to inhibit high glucose damage. The protective effects and mechanisms of a novel Lentinus edodes mycelia polysaccharide (LMP) against damage induced by high glucose in MIN6 cells were explored.

Methods: Cell viability, malondialdehyde (MDA) inhibition, lactate dehydrogenase (LDH) release and the activity of superoxide dismutase (SOD) were evaluated under 40 mM glucose with or without LMP for 48 h. Cell signaling pathway analysis was performed to investigate the possible mechanisms of the protective effects of LMP in MIN6 cells.

Results: The results showed that LMP could increase cell viability and the activity of SOD, decrease the reactive oxygen species ( ROS) production, and reduce the MDA content and LDH release in high glucose-induced MIN6 cells. Moreover, LMP prevented high glucose-induced apoptosis by decreasing the expression of Bax and the activation of caspase-1 and caspase-3. Cell signaling pathway analysis showed that p38 mitogen-activated protein kinase (MAPK) and JNK pathways were inhibited and the Nrf2 pathway was activated after treated with LMP.

Conclusion: The protective effects of LMP against MIN6 cells damage induced by high glucose might rely on the regulation of the MAPK and Nrf2 pathways. These results indicated that LMP had great potential as a therapeutic agent for the treatment of diabetes mellitus.
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http://dx.doi.org/10.29219/fnr.v63.1598DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6560380PMC
June 2019