Publications by authors named "Jianjun Yang"

367 Publications

miR‑483 promotes the development of colorectal cancer by inhibiting the expression level of EI24.

Mol Med Rep 2021 Aug 10;24(2). Epub 2021 Jun 10.

Department of Gastrointestinal Surgery, Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.

MicroRNAs (miRs) serve an important role in cell differentiation, proliferation and apoptosis by negatively regulating gene expression at the transcriptional or post‑transcriptional level. EI24 autophagy associated transmembrane protein (EI24) is a tumor suppressor gene that serves an important role in the occurrence and development of digestive system tumors. However, little is known regarding the relationship between EI24 and the prognosis of patients with colorectal cancer (CRC). Our previous study confirmed EI24 as the target molecule of miR‑483, using reporter gene detection. Thus, the aim of the present study was to elucidate the effect of the abnormal expression of miR‑483 on the malignant phenotype of CRC through a series of cell function experiments and nude mice tumorigenicity experiments, and to determine the expression level of EI24, a downstream target gene of miR‑483, in CRC and its relationship with patient prognosis. In CRC tissues and cells, the expression level of miR‑483 was upregulated, while the expression level of EI24 was downregulated. Cell function tests such as MTT assay, cell cycle assay, colony formation assay, Migration and invasion assays and nude mice tumorigenicity experiments demonstrated that the overexpression of miR‑483 promoted the proliferation, invasion and metastasis of CRC. Moreover, the reverse transcription‑quantitative PCR results indicated that overexpression of miR‑483 inhibited the expression level of EI24. The relationship between the clinical data and immunohistochemical results from 183 patients with CRC and survival was examined. It was found that the expression level of EI24 was positively associated with the prognosis of patients. As a cancer‑promoting factor, miR‑483 enhances the proliferation, migration and invasion of CRC cells by reducing the expression level of EI24.
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http://dx.doi.org/10.3892/mmr.2021.12206DOI Listing
August 2021

Sex-Based Differences in Clinical Outcomes After Arthroscopic Anterior Shoulder Stabilization: Results at 5-Year Follow-up.

Orthop J Sports Med 2021 May 20;9(5):23259671211008841. Epub 2021 May 20.

Sports Medicine Institute, Department of Sports Medicine and Arthroscopy, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.

Background: The influence of patient sex on clinical outcomes after arthroscopic anterior shoulder stabilization is unclear.

Purpose: To investigate sex-based differences in clinical outcomes after arthroscopic anterior shoulder stabilization.

Study Design: Cohort study; Level of evidence, 3.

Methods: A retrospective study was conducted among 76 patients who underwent arthroscopic anterior shoulder stabilization from February 2010 to December 2017. The patients were grouped by sex. The recurrence rate of instability was compared, as were pre- and postoperative pain scores, functional outcome scores, and active range of motion (ROM). Postoperative magnetic resonance imaging (MRI) was also performed for structural assessment of the glenohumeral joint.

Results: No significant difference was found in the recurrence rate between female and male patients (13.3% vs 14.8%; ≥ .999; risk ratio, 1.107 [95% CI, 0.266-4.597]). Compared with male patients, female patients had a significantly lower preoperative Constant score (94.4 ± 6.4 vs 85.4 ± 11.1; = .002), forward flexion (173.8° ± 10.7° vs 154.0° ± 33.8°; = .011), abduction (171.0° ± 18.4° vs 142.7° ± 39.9°; = .001), and external rotation (76.6° ± 21.6° vs 67.7° ± 20.4°; = .037). Postoperatively, female patients had a significantly lower Simple Shoulder Test score compared with men (8.8 ± 1.9 vs 10.3 ± 1.6; = .005). The mean changes from pre- to postoperative Rowe score (43.6 ± 21.4 vs 31.5 ± 19.8; = .044), Constant score (9.9 ± 8.9 vs 0.8 ± 8.1; = .002), forward flexion (24.0 ± 36.2 vs 4.2 ± 10.9; = .013), abduction (36.0 ± 38.3 vs 7.6 ± 18.4; < .001), and external rotation (19.7 ± 21.3 vs 6.7 ± 26.2; = .023) in female patients were significantly higher than those in the men. There were no sex-based differences on any MRI parameter measured.

Conclusion: Female patients had a similar recurrence rate as that of male patients after arthroscopic anterior shoulder stabilization. Most postoperative clinical outcome measures showed no significant difference between the sexes. Despite worse preoperative values, more significant improvements in postoperative shoulder function and active ROM were seen in women.
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http://dx.doi.org/10.1177/23259671211008841DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142006PMC
May 2021

Dual-state emission difluoroboron derivatives for selective detection of picric acid and reversible acid/base fluorescence switching.

Anal Methods 2021 Jun 3. Epub 2021 Jun 3.

College of Chemistry and Chemical Engineering, Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functional Materials, Anhui University, Hefei 230601, P. R. China.

A novel difluoroboron derivative (TPEBF) containing α-cyanostilbene and tetraphenylethylene units has been designed and synthesized. TPEBF emits strong fluorescence both in dilute solutions (ΦFL = 19.3% in THF) and in the solid state (ΦFL = 49.3%), which is significantly distinct from the case of the aggregation-caused quenching (ACQ) and aggregation-induced emission (AIE) chromophores. The dual-state emission properties of the compound overcome the limitation of single-state luminescence and enable it to be used in both solid and solution states. TPEBF with strong emission in solution is utilized for sensing picric acid (PA) with high selectivity and sensitivity in THF (LOD = 497 nM) and aqueous media (LOD = 355 nM). The mechanism was described for the synergy of fluorescence resonance energy transfer (FRET) and photoinduced energy transfer (PET) based on the UV-vis absorption and fluorescence spectra, 1H NMR and theoretical calculations results. On the other hand, the highly efficient emission in the solid state allows the compound to be cast on paper to switch external acid/base stimuli.
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http://dx.doi.org/10.1039/d1ay00477hDOI Listing
June 2021

Design of Driving Waveform for Shortening Red Particles Response Time in Three-Color Electrophoretic Displays.

Micromachines (Basel) 2021 May 19;12(5). Epub 2021 May 19.

South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou 510006, China.

Three-color electrophoretic displays (EPDs) have the advantages of multi-color display and low power consumption. However, their red particles have the disadvantage of long response time. In this paper, a driving waveform, which is based on electrophoresis theory and reference gray scale optimization, was proposed to shorten the response time of red particles in three-color EPDs. The driving waveform was composed of erasing stage, reference gray scale forming stage, red driving stage, and white or black driving stage. Firstly, the characteristics of particle motion were analyzed by electrophoresis theory and Stokes law. Secondly, the reference gray scale of the driving waveform was optimized to shorten the distance between red particles and a common electrode plate. Finally, an experimental platform was developed to test the performance of the driving waveform. Experimental results showed that the proposed driving waveform can shorten the response time of red particles by 65.57% and reduce the number of flickers by 66.67% compared with the traditional driving waveform.
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http://dx.doi.org/10.3390/mi12050578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161037PMC
May 2021

Biobased Plasticizers from Tartaric Acid: Synthesis and Effect of Alkyl Chain Length on the Properties of Poly(vinyl chloride).

ACS Omega 2021 May 16;6(20):13161-13169. Epub 2021 May 16.

School of Chemistry and Chemical Engineering, Anhui University, Hefei 230601, China.

A series of tartaric acid (TA) esters with different side chain lengths [dibutyl TA esters (DBTAE)-C], as plasticizers for poly(vinyl chloride) (PVC), is herein reported. Their structures have been fully characterized using proton nuclear magnetic resonance and Fourier-transform infrared spectroscopy. Their compatibility and plasticizing effect for soft PVC were evaluated using thermogravimetric analysis, dynamic mechanical analysis, tensile testing, and migration testing. The results showed that all these TA esters exhibit good plasticizing performance. At a concentration of 30 phr in PVC, the best results for the plasticizing effect, in terms of glass transition temperature reduction and elongation at break, were achieved when the ester DBTAE-C4 was used. However, the longer side chains of these esters improved the thermal stability of soft PVC blends yet exacerbated the migration behavior of these esters from PVC films in -hexane. The properties of the plasticized PVC blends depended on the structural features of DBTAE-C. The plasticizing performances of the esters DBTAE-C1 and DBTAE-C4 rivaled that of dioctyl phthalate (DOP), suggesting that they have the potential to replace DOP in soft PVC materials.
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http://dx.doi.org/10.1021/acsomega.1c01006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158838PMC
May 2021

Robust Carbonated Structural Color Barcodes with Ultralow Ontology Fluorescence as Biomimic Culture Platform.

Research (Wash D C) 2021 4;2021:9851609. Epub 2021 May 4.

Key Laboratory for Biomechanics and Mechanobiology, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China.

Photonic crystal (PC) barcodes are a new type of spectrum-encoding microcarriers used in multiplex high-throughput bioassays, such as broad analysis of biomarkers for clinical diagnosis, gene expression, and cell culture. Unfortunately, most of these existing PC barcodes suffered from undesired features, including difficult spectrum-signal acquisition, weak mechanical strength, and high ontology fluorescence, which limited their development to real applications. To address these limitations, we report a new type of structural color-encoded PC barcodes. The barcodes are fabricated by the assembly of monodisperse polydopamine- (PDA-) coated silica ([email protected]) nanoparticles using a droplet-based microfluidic technique and followed by pyrolysis of [email protected] ([email protected]) barcodes. Because of the templated carbonization of adhesive PDA, the prepared [email protected] PC beads were endowed with simultaneous easy-to-identify structural color, high mechanical strength, and ultralow ontology fluorescence. We demonstrated that the structural colored [email protected] barcodes not only maintained a high structural stability and good biocompatibility during the coculturing with fibroblasts and tumor cells capture but also achieved an enhanced fluorescent-reading signal-to-noise ratio in the fluorescence-reading detection. These features make the [email protected] PC barcodes versatile for expansive application in fluorescence-reading-based multibioassays.
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http://dx.doi.org/10.34133/2021/9851609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118130PMC
May 2021

Propagation Model of Panic Buying Under the Sudden Epidemic.

Front Public Health 2021 22;9:675687. Epub 2021 Apr 22.

School of Tourism and Urban-Rural Planning, Zhejiang Gongshang University, Hangzhou, China.

The sudden outbreak of COVID-19 at the end of 2019 has had a huge impact on people's lives all over the world, and the overwhelmingly negative information about the epidemic has made people panic for the future. This kind of panic spreads and develops through online social networks, and further spreads to the offline environment, which triggers panic buying behavior and has a serious impact on social stability. In order to quantitatively study this behavior, a two-layer propagation model of panic buying behavior under the sudden epidemic is constructed. The model first analyzes the formation process of individual panic from a micro perspective, and then combines the Susceptible-Infected-Recovered (SIR) Model to simulate the spread of group behavior. Then, through simulation experiments, the main factors affecting the spread of panic buying behavior are discussed. The experimental results show that: (1) the dissipating speed of individual panics is related to the number of interactions and there is a threshold. When the number of individuals involved in interacting is equal to this threshold, the panic of the group dissipates the fastest, while the dissipation speed is slower when it is far from the threshold; (2) The reasonable external information release time will affect the occurrence of the second panic buying, meaning providing information about the availability of supplies when an escalation of epidemic is announced will help prevent a second panic buying. In addition, when the first panic buying is about to end, if the scale of the second panic buying is to be suppressed, it is better to release positive information after the end of the first panic buying, rather than ahead of the end; and (3) Higher conformity among people escalates panic, resulting in panic buying. Finally, two cases are used to verify the effectiveness and feasibility of the proposed model.
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http://dx.doi.org/10.3389/fpubh.2021.675687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100230PMC
May 2021

High-Performance CVD Bilayer MoS Radio Frequency Transistors and Gigahertz Mixers for Flexible Nanoelectronics.

Micromachines (Basel) 2021 Apr 16;12(4). Epub 2021 Apr 16.

School of Electronic Information, University of Electronic Science and Technology of China, Zhongshan Institute, Zhongshan 528402, China.

Two-dimensional (2D) MoS have attracted tremendous attention due to their potential applications in future flexible high-frequency electronics. Bilayer MoS exhibits the advantages of carrier mobility when compared with monolayer mobility, thus making the former more suitable for use in future flexible high-frequency electronics. However, there are fewer systematical studies of chemical vapor deposition (CVD) bilayer MoS radiofrequency (RF) transistors on flexible polyimide substrates. In this work, CVD bilayer MoS RF transistors on flexible substrates with different gate lengths and gigahertz flexible frequency mixers were constructed and systematically studied. The extrinsic cutoff frequency () and maximum oscillation frequency () increased with reducing gate lengths. From transistors with a gate length of 0.3 μm, we demonstrated an extrinsic of 4 GHz and of 10 GHz. Furthermore, statistical analysis of 14 flexible MoS RF transistors is presented in this work. The study of a flexible mixer demonstrates the dependence of conversion gain versus gate voltage, LO power and input signal frequency. These results present the potential of CVD bilayer MoS for future flexible high-frequency electronics.
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http://dx.doi.org/10.3390/mi12040451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072592PMC
April 2021

High-speed femtosecond laser plasmonic lithography and reduction of graphene oxide for anisotropic photoresponse.

Light Sci Appl 2020 Apr 26;9(1):69. Epub 2020 Apr 26.

State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, 130033, Changchun, China.

Micro/nanoprocessing of graphene surfaces has attracted significant interest for both science and applications due to its effective modulation of material properties, which, however, is usually restricted by the disadvantages of the current fabrication methods. Here, by exploiting cylindrical focusing of a femtosecond laser on graphene oxide (GO) films, we successfully produce uniform subwavelength grating structures at high speed along with a simultaneous in situ photoreduction process. Strikingly, the well-defined structures feature orientations parallel to the laser polarization and significant robustness against distinct perturbations. The proposed model and simulations reveal that the structure formation is based on the transverse electric (TE) surface plasmons triggered by the gradient reduction of the GO film from its surface to the interior, which eventually results in interference intensity fringes and spatially periodic interactions. Further experiments prove that such a regular structured surface can cause enhanced optical absorption (>20%) and an anisotropic photoresponse (~0.46 ratio) for the reduced GO film. Our work not only provides new insights into understanding the laser-GO interaction but also lays a solid foundation for practical usage of femtosecond laser plasmonic lithography, with the prospect of expansion to other two-dimensional materials for novel device applications.
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http://dx.doi.org/10.1038/s41377-020-0311-2DOI Listing
April 2020

Differential transformation mechanisms of exotic Cr(VI) in agricultural soils with contrasting physio-chemical and biological properties.

Chemosphere 2021 Apr 13;279:130546. Epub 2021 Apr 13.

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, 100081, PR China.

The transformation mechanisms of Cr(VI) in agricultural soils at the molecular level remain largely unknown due to the multitude of abiotic and biotic factors. In this study, the different speciation and distribution of Cr in two types of agricultural soil (Ultisol and Fluvo-aquic soils) after two weeks of aging was investigated using synchrotron-based X-ray absorption near-edge structure (XANES) spectroscopy, microfocused X-ray fluorescence (μ-XRF) and X-ray transmission microscopy (STXM). The microbial community structure of the two soils was also analyzed via high-throughput sequencing of 16S rRNA. Cr(VI) availability was relatively lower in the Ultisol than in the Fluvo-aquic soil after aging. Cr K-edge bulk XANES and STXM analysis indicated that Cr(VI) was reduced to Cr(III) in both soils. μ-XRF analysis and STXM analysis indicated the predominant association of Cr with Mn/Fe oxides and/or organo-Fe oxides in both soils. Additionally, STXM-coupled imaging and multiedge XANES analyses demonstrated that carboxylic groups were involved in the reduction of Cr(VI) and subsequent retention of Cr(III). 16S rRNA analysis showed considerably different bacterial communities across the two soils. Redundancy analysis (RDA) suggested that soil properties, including the total carbon content, Fe oxide component and pH, were closely linked to Cr(VI)-reducing functional bacteria in the Ultisol, including chromium-reducing bacteria (CRB) (e.g., Bacillus sp.) and dissimilatory iron-reducing (DIRB) (e.g., Shewanella sp.) bacteria, which possibly promoted Cr(VI) reduction. These findings shed light on the molecular-level transformation mechanisms of Cr(VI) in agricultural soils, which facilitates the effective management of Cr-enriched farmland.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130546DOI Listing
April 2021

Templateless, Plating-Free Fabrication of Flexible Transparent Electrodes with Embedded Silver Mesh by Electric-Field-Driven Microscale 3D Printing and Hybrid Hot Embossing.

Adv Mater 2021 May 7;33(21):e2007772. Epub 2021 Apr 7.

Shandong Engineering Research Center for Additive Manufacturing, Qingdao University of Technology, Qingdao, 266520, China.

Flexible transparent electrodes (FTEs) with an embedded metal mesh are considered a promising alternative to traditional indium tin oxide (ITO) due to their excellent photoelectric performance, surface roughness, and mechanical and environmental stability. However, great challenges remain for achieving simple, cost-effective, and environmentally friendly manufacturing of high-performance FTEs with embedded metal mesh. Herein, a maskless, templateless, and plating-free fabrication technique is proposed for FTEs with embedded silver mesh by combining an electric-field-driven (EFD) microscale 3D printing technique and a newly developed hybrid hot-embossing process. The final fabricated FTE exhibits superior optoelectronic properties with a transmittance of 85.79%, a sheet resistance of 0.75 Ω sq , a smooth surface of silver mesh (R  ≈ 18.8 nm) without any polishing treatment, and remarkable mechanical stability and environmental adaptability with a negligible increase in sheet resistance under diverse cyclic tests and harsh working conditions (1000 bending cycles, 80 adhesion tests, 120 scratch tests, 100 min ultrasonic test, and 72 h chemical attack). The practical viability of this FTE is successfully demonstrated with a flexible transparent heater applied to deicing. The technique proposed offers a promising fabrication strategy with a cost-effective and environmentally friendly process for high-performance FTE.
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http://dx.doi.org/10.1002/adma.202007772DOI Listing
May 2021

An Antifungal Chitosanase from SH21.

Molecules 2021 Mar 25;26(7). Epub 2021 Mar 25.

Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticides, Beijing Laboratory for Food Quality and Safety, Beijing Engineering Laboratory of Probiotics Key Technology Development, Beijing Engineering Technology Research Center of Food Safety Immune Rapid Detection, Food Science and Engineering College, Beijing University of Agriculture, Beijing 102206, China.

SH21 was observed to produce an antifungal protein that inhibited the growth of . To purify this protein, ammonium sulfate precipitation, gel filtration chromatography, and ion-exchange chromatography were used. The purity of the purified product was 91.33% according to high-performance liquid chromatography results. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis revealed that the molecular weight of the protein is 30.72 kDa. The results of the LC-MS/MS analysis and a subsequent sequence-database search indicated that this protein was a chitosanase, and thus, we named it chitosanase SH21. Scanning and transmission electron microscopy revealed that chitosanase SH21 appeared to inhibit the growth of by causing hyphal ablation, distortion, or abnormalities, and cell-wall depression. The minimum inhibitory concentration of chitosanase SH21 against was 68 µg/mL. Subsequently, the corresponding gene was cloned and sequenced, and sequence analysis indicated an open reading frame of 831 bp. The predicted secondary structure indicated that chitosanase SH21 has a typical a-helix from the glycoside hydrolase (GH) 46 family. The tertiary structure shared 40% similarity with that of N174. This study provides a theoretical basis for a topical cream against fungal infections in agriculture and a selection marker on fungi.
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http://dx.doi.org/10.3390/molecules26071863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036696PMC
March 2021

Magnetic nanocarriers as a therapeutic drug delivery strategy for promoting pain-related motor functions in a rat model of cartilage transplantation.

J Mater Sci Mater Med 2021 Mar 31;32(4):37. Epub 2021 Mar 31.

Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, China.

Cartilage is an avascular tissue with low cellularity and insufficient self-repair response. In clinical practice, a large articular cartilage defect is usually fixed by cartilage transplantation. Importantly, the fast repair process has been demanded postoperatively in the area between the host cartilage and the transplanted cartilage. In the past few years, magnetic nanoparticles have drawn great attention due to their biocompatible, biodegradable, and nontoxic properties. In addition, the nanoparticles can easily pass through the cell plasma membrane and increase the cellular uptake efficiency. Here, a therapeutic drug delivery strategy was proposed for cartilage repair. The prepared kartogenin (KGN)-conjugated magnetic nanocarriers ([email protected]) promoted the viability of chondrocytes in vitro. In a rat model of cartilage transplantation, intra-articularly delivered [email protected] generated cartilage with a flat surface and a high level of aggrecan in vivo. Notably, [email protected] were also capable of improving the pain-related motor functions. They promoted the motor functional parameters including the print area and intensity to restore to a normal level compared with the single KGN. Therefore, these therapeutic drug nanocarriers provided the potential for cartilage repair.
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http://dx.doi.org/10.1007/s10856-021-06508-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012338PMC
March 2021

Modeling, simulation, and case analysis of COVID-19 over network public opinion formation with individual internal factors and external information characteristics.

Concurr Comput 2021 Jan 23:e6201. Epub 2021 Jan 23.

School of Tourism and Urban-Rural Planning Zhejiang Gongshang University Hangzhou China.

With the development of information technology, the Internet has become an important channel of public opinion for expressing public interests, emotion, and ideas. Public emergency usually spreads via network. Due to the temporal and spatial flexibility and the information amplification of network, the opinions from different regions and background are easy to be represented as network public opinion, and have important impact on social and economic life. Thus, studying the formation mechanism of network public opinion has important theoretical and practical significance. Taking the formation process of network public opinion under emergencies as the research object, this paper first identifies the key factors influencing the formation of network public opinion, namely the internal characteristics (include individual education level, individual stubbornness, individual initial opinion, and so on) and external information of individuals (include external information intensity). Second, information intensity is introduced to describe the influence of external information feature on the formation of network public opinion. Individual education level, individual stubbornness, and individual initial opinion are analyzed to describe the influence of individual internal factors on the formation, and then its model is constructed. Through the simulation experiments, this paper analyzes the influence of external information intensity, individual education level, individual stubbornness, individual initial opinion, and other factors on the formation of network public opinion. The simulation results show that: (1) the greater intensity of public emergency reporting causes the easier formation of network public opinion; (2) the higher individual education level leads to the shorter time for completing the final formation and stable state of online public opinions, and after the formation of online public opinions, the opinion of the event is mainly neutral; (3) the greater individual's stubbornness makes the shorter formation time of online public opinion. When online public opinion reaches a stable state, the neutral opinion group dominates and firmly controls the development trend of public opinion; (4) the difference of opinions among individuals is the most important factor affecting the formation of network public opinion. Finally, the rationality and validity of the proposed model are verified by a real case. Compared with previous studies on the formation mechanism of network public opinion, this paper divides the formation process of network public opinion into three stages: individual information perception, individual decision making, and individual opinion transmission. Meanwhile, the influence of individual internal factors and external information characteristics on the formation process of network public opinion is also considered.
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http://dx.doi.org/10.1002/cpe.6201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995027PMC
January 2021

Delivery of gefitinib with an immunostimulatory nanocarrier improves therapeutic efficacy in lung cancer.

Transl Lung Cancer Res 2021 Feb;10(2):926-935

Department of Thoracic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: Combining different cancer treatments represents a promising strategy to improve the therapeutic outcome for lung cancer patients with or without druggable gene alterations.

Methods: We previously developed a polyethylene glycol-based (PEG-based) immunostimulatory nanocarrier (PEG-Fmoc-NLG919) which can efficiently co-deliver an indoleamine 2,3-dioxygenase-1 (IDO1) inhibitor and the chemotherapeutic agent, paclitaxel. This method was found to improve cancer therapy by simultaneously performing immuno- and chemo-therapy. However, whether this nanocarrier could deliver targeted drugs to implement targeted therapy together with immunotherapy remains unclear.

Results: Here, we report that the delivery of the classical tyrosine kinase inhibitor (TKI), gefitinib, with the optimized PEG-Fmoc-NLG919 nanocarrier, increased the sensitivity of lung cancer cells to gefitinib . Gefitinib was gradually but sufficiently released from the nanocarrier with comparable capacity to inhibit epidermal growth factor receptor (EGFR) activity as using free gefitinib directly. More importantly, treatment with gefitinib-loaded PEG-Fmoc-NLG919 could suppress lung tumor development more efficiently than gefitinib alone by inducing an immune active microenvironment with more functional CD8 T cells and less regulatory T cell infiltration.

Conclusions: Our study therefore demonstrates that delivery of small molecular targeted drugs with the immunostimulatory nanocarrier is a straightforward strategy for improving antitumor response for lung cancer therapy.
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http://dx.doi.org/10.21037/tlcr-21-144DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947404PMC
February 2021

Mechanisms of Enterobacter bugandensis TJ6 immobilization of heavy metals and inhibition of Cd and Pb uptake by wheat based on metabolomics and proteomics.

Chemosphere 2021 Aug 4;276:130157. Epub 2021 Mar 4.

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, 100081, PR China. Electronic address:

Microbial passivation remediation of heavy metal-contaminated farmland has attracted increasing attention. However, the molecular mechanism by which heavy metal-immobilizing bacteria inhibit the uptake of Cd and Pb by wheat is not clear. Herein, a heavy metal-immobilizing bacterium, Enterobacter bugandensis TJ6, was used to reveal its immobilization mechanisms of Cd and Pb and inhibition of Cd and Pb uptake by wheat using metabolomics and proteomics. Compared with the control, strain TJ6 significantly reduced (44.7%-56.6%) the Cd and Pb contents of wheat roots and leaves. Strain TJ6 reduced the Cd and Pb concentrations by adsorption, intracellular accumulation, and bioprecipitation in solution. Untargeted metabolomics showed that strain TJ6 produced indole-3-acetic acid (IAA), betaine, and arginine under Cd and Pb stress, significantly improving the resistance of strain TJ6 and wheat to Cd and Pb. Label-free proteomics showed that 143 proteins were upregulated and 61 proteins were downregulated in wheat roots in the presence of strain TJ6. The GO items of the differentially expressed proteins (DEPs) involved in protein-DNA complexes, DNA packaging complexes, and peroxidase activity were enriched. In addition, the ability of wheat roots to synthesize abscisic acid and jasmonic acid was improved. In conclusion, strain TJ6 reduced Cd and Pb uptake in wheat through its own adsorption of Cd and Pb and regulation of wheat root DNA repair ability, plant hormone levels, and antioxidant activities. These results provide new insights and a theoretical basis for the application of heavy metal-immobilizing bacteria in safe wheat production.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130157DOI Listing
August 2021

Spatially Separating Redox Centers and Photothermal Effect Synergistically Boosting the Photocatalytic Hydrogen Evolution of ZnIn S Nanosheets.

Small 2021 Apr 11;17(17):e2006952. Epub 2021 Mar 11.

National & Local Joint Engineering Research Center for Applied Technology of Hybrid Nanomaterials, Henan University, Kaifeng, Henan, 475004, China.

Spatially separated loading of reductive and oxidative cocatalysts is a useful strategy for expediting charge separation and surface reaction kinetics, which are two key factors for determining the photocatalytic efficiency. However, loading the spatial separation of dual cocatalysts on a 2D photocatalyst is still a great challenge. Herein, decorating the spatial separation of oxidative and reductive cocatalysts on ZnIn S nanosheets is realized by designing a ternary Co S @ZnIn S @PdS ([email protected]@PS) hollow tubular core-shell structure. Particularly, Co S and PdS functionally serve as the reduction and oxidation cocatalysts, respectively. Experimental results confirm that the spatial separation of Co S and PdS cocatalysts not only efficiently improve charge separation and accelerate surface reduction-oxidation kinetics, but also generate a photothermal effect to further enhance charge transfer and surface reaction kinetics. As a result, the optimized [email protected]@PS yields a remarkable H evolution rate of 11407 µmol g h , and the apparent quantum efficiency reaches 71.2% at 420 nm, which is one of the highest values among ZnIn S so far. The synergistic effect of spatially separated dual cocatalysts and photothermal effect may be applied to other 2D materials for efficient solar energy conversion.
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http://dx.doi.org/10.1002/smll.202006952DOI Listing
April 2021

Paraventricular thalamic nucleus plays a critical role in consolation and anxious behaviors of familiar observers exposed to surgery mice.

Theranostics 2021 6;11(8):3813-3829. Epub 2021 Feb 6.

Department of Anesthesiology, University of Virginia, Charlottesville, VA 22908, U.S.A.

Consolation behaviors toward the sick are common in humans. Anxiety in the relatives of the sick is also common. Anxiety can cause detrimental effects on multiple systems. However, our understanding on the neural mechanisms of these behaviors is limited because of the lack of small animal models. Five of 6- to 8-week-old CD-1 male mice were housed in a cage. Among them, 2 mice had right common artery exposure (surgery) and the rest were without surgery. Allo-grooming and performance in light and dark box and elevated plus maze tests of the mice were determined. Mice without surgery had increased allo-grooming toward mice with surgery but decreased allo-grooming toward non-surgery intruders. This increased allo-grooming toward surgery mice was higher in familiar observers of surgery mice than that of mice that were not cage-mates of surgery mice before the surgery. Familiar observers developed anxious behavior after being with surgery mice. Surgery mice with familiar observers had less anxious behavior than surgery mice without interacting with familiar observers. Multiple brain regions including paraventricular thalamic nucleus (PVT) were activated in familiar observers. The activated cells in PVT contained orexin receptors. Injuring the neurons with ibotenic acid, antagonizing orexin signaling with an anti-orexin antibody or inhibiting neurons by chemogenetic approach in PVT abolished the consolation and anxious behaviors of familiar observers. Mice show consolation behavior toward the sick. This behavior attenuates the anxious behavior of surgery mice. The orexin signaling in the PVT neurons play a critical role in the consolation of familiar observers toward surgery mice and their anxious behavior. Considering that about 50 million patients have surgery annually in the United States, our study represents the initial attempt to understand neural mechanisms for consolation and anxiety of a large number of people.
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http://dx.doi.org/10.7150/thno.45690DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914349PMC
February 2021

Frailty is an independent risk factor of one-year mortality after elective orthopedic surgery: a prospective cohort study.

Aging (Albany NY) 2021 02 26;13(5):7190-7198. Epub 2021 Feb 26.

Department of Anesthesiology, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China.

Frailty is associated with perioperative adverse outcomes, especially for the elderly. This study aimed to assess whether frailty was an independent risk factor of one-year mortality in frail patients after elective orthopedic surgery. In this prospective study, three hundred and thirteen patients aged ≥ 65 years, undergoing elective orthopedic surgery were finally included. Frailty assessed by the Clinical Frailty Score (CFS) before the surgery was present in 29.7% (93/313). Among them, 7.7% of patients (24/313) died at one year after surgery. In multivariate logistic analysis, higher CFS (OR = 2.271, 95% CI= 1.472-3.504) was found to be an independent risk factor of one-year mortality after surgery in elderly orthopedic patients. The area under the receiver operating characteristic curve of the model was 0.897 (95% CI 0.834-0.959). In addition, we found higher Charlson comorbidity index (OR= 1.498, 95% CI = 1.082-2.073) was also a significant risk factor. In conclusion, frailty is associated with increased one-year mortality in elderly patients after elective orthopedic surgery, which should be considered as a routine assessment tool in preoperative practice.
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http://dx.doi.org/10.18632/aging.202576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993662PMC
February 2021

Less Social Support for Patients With COVID-19: Comparison With the Experience of Nurses.

Front Psychiatry 2021 1;12:554435. Epub 2021 Feb 1.

Department of Anesthesiology and Perioperative Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Since December 2019, more than 80,000 patients have been diagnosed with coronavirus disease 2019 (COVID-19) in China. Social support status of COVID-19 patients, especially the impact of social support on their psychological status and quality of life, needs to be addressed with increasing concern. In this study, we used social support rating scale (SSRS) to investigate the social support in COVID-19 patients and nurses. The present study included 186 COVID-19 patients at a Wuhan mobile cabin hospital and 234 nurses at a Wuhan COVID-19 control center. Responses to a mobile phone app-based questionnaire about social support, anxiety, depression, and quality of life were recorded and evaluated. COVID-19 patients scored significantly lower than nurses did on the Social Support Rating Scale (SSRS). Among these patients, 33.9% had anxiety symptoms, while 23.7% had depression symptoms. Overall SSRS, subjective social support scores and objective support scores of patients with anxiety were lower than those of patients without anxiety. This result was also found in depression. In addition, all dimensions of social support were positively correlated with quality of life. Interestingly, in all dimensions of social support, subjective support was found to be an independent predictive factor for anxiety, depression, and quality of life, whereas objective support was a predictive factor for quality of life, but not for anxiety and depression via regression analysis. Medical staffs should pay attention to the subjective feelings of patients and make COVID-19 patients feel respected, supported, and understood from the perspective of subjective support, which may greatly benefit patients, alleviate their anxiety and depression, and improve their quality of life.
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http://dx.doi.org/10.3389/fpsyt.2021.554435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901979PMC
February 2021

Association of systemic inflammation and body mass index with survival in patients with resectable gastric or gastroesophageal junction adenocarcinomas.

Cancer Biol Med 2021 Feb;18(1):283-297

State Key Laboratory of Cancer Biology and National Clinical Research Center for Digestive Diseases, Xijing Hospital, Air Force Medical University of PLA, Xi'an 710032, China.

Objective: The systemic inflammation index and body mass index (BMI) are easily accessible markers that can predict mortality. However, the prognostic value of the combined use of these two markers remains unclear. The goal of this study was therefore to evaluate the association of these markers with outcomes based on a large cohort of patients with gastric cancer.

Methods: A total of 2,542 consecutive patients undergoing radical surgery for gastric or gastroesophageal junction adenocarcinoma between 2009 and 2014 were included. Systemic inflammation was quantified by the preoperative neutrophil-to-lymphocyte ratio (NLR). High systemic inflammation was defined as NLR ≥ 3, and underweight was defined as BMI < 18.5 kg/m.

Results: Among 2,542 patients, NLR ≥ 3 and underweight were common [627 (25%) and 349 (14%), respectively]. In the entire cohort, NLR ≥ 3 or underweight independently predicted overall survival (OS) [hazard ratio (HR): 1.236, 95% confidence interval (95% CI): 1.069-1.430; and HR: 1.600, 95% CI: 1.350-1.897, respectively] and recurrence-free survival (RFS) (HR: 1.230, 95% CI: 1.054-1.434; and HR: 1.658, 95% CI: 1.389-1.979, respectively). Patients with both NLR ≥ 3 and underweight ( neither) had much worse OS (HR: 2.445, 95% CI: 1.853-3.225) and RFS (HR: 2.405, 95% CI: 1.802-3.209). Furthermore, we observed similar results in subgroup analyses according to pathological stage, age, and postoperative chemotherapy.

Conclusions: Our results showed that preoperative elevated NLR and decreased BMI had a significant negative effect on survival. Underweight combined with severe inflammation could enhance prognostication. Taking active therapeutic measures to reduce inflammation and increase nutrition may help improve outcomes.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0246DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877168PMC
February 2021

RIPK3 Activates MLKL-mediated Necroptosis and Inflammasome Signaling during Infection.

Am J Respir Cell Mol Biol 2021 May;64(5):579-591

Department of Medicine, Pulmonary and Critical Care, Weill Cornell Medicine, New York, New York.

Community-acquired pneumonia is the most common type of pneumonia and remains a leading cause of morbidity and mortality worldwide. Although many different pathogens can contribute to pneumonia, is one of the common bacterial pathogens that underlie community-acquired pneumonia. RIPK3 (receptor-interacting protein kinase 3) is widely recognized as a key modulator of inflammation and cell death. To elucidate a potential role of RIPK3 in pneumonia, we examined plasma from healthy control subjects and patients positive for streptococcal pneumonia. In human studies, RIPK3 protein concentrations were significantly elevated and were identified as a potential plasma marker of pneumococcal pneumonia. To expand these findings, we used an murine model of pneumococcal pneumonia to demonstrate that RIPK3 deficiency leads to reduced bacterial clearance, severe pathological damage, and high mortality. Our results illustrated that RIPK3 forms a complex with RIPK1, MLKL (mixed-lineage kinase domain-like protein), and MCU (mitochondrial calcium uniporter) to induce mitochondrial calcium uptake and mitochondrial reactive oxygen species(mROS) production during infection. In macrophages, RIPK3 initiated necroptosis via the mROS-mediated mitochondrial permeability transition pore opening and NLRP3 inflammasome activation via the mROS-AKT pathway to protect against . In conclusion, our study demonstrated a mechanism by which RIPK3-initiated necroptosis is essential for host defense against .
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http://dx.doi.org/10.1165/rcmb.2020-0312OCDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086037PMC
May 2021

Combining Public Opinion Dissemination with Polarization Process Considering Individual Heterogeneity.

Healthcare (Basel) 2021 Feb 7;9(2). Epub 2021 Feb 7.

Hubei Key Laboratory of Mechanical Transmission and Manufacturing Engineering, Wuhan University of Science and Technology, Wuhan 430081, China.

The wide dissemination of false information and the frequent occurrence of extreme speeches on online social platforms have become increasingly prominent, which impact on the harmony and stability of society. In order to solve the problems in the dissemination and polarization of public opinion over online social platforms, it is necessary to conduct in-depth research on the formation mechanism of the dissemination and polarization of public opinion. This article appends individual communicating willingness and forgetting effects to the Susceptible-Exposed-Infected-Recovered (SEIR) model to describe individual state transitions; secondly, it introduces three heterogeneous factors describing the characteristics of individual differences in the Jager-Amblard (J-A) model, namely: Individual conformity, individual conservative degree, and inter-individual relationship strength in order to reflect the different roles of individual heterogeneity in the opinions interaction; thirdly, it integrates the improved SEIR model and J-A model to construct the SEIR-JA model to study the formation mechanism of public opinion dissemination and polarization. Transmission parameters and polarization parameters are simulated and analyzed. Finally, a public opinion event from the pricing of China's self-developed COVID-19 vaccine are used, and related Weibo comment data about this event are also collected so as to verify the rationality and effectiveness of the proposed model.
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http://dx.doi.org/10.3390/healthcare9020176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915404PMC
February 2021

Optimised anthropometric indices as predictive screening tools for metabolic syndrome in adults: a cross-sectional study.

BMJ Open 2021 01 31;11(1):e043952. Epub 2021 Jan 31.

School of Public Health and Management, Ningxia Medical University, Yinchuan, China

Objectives: We aimed to evaluate the association between metabolic syndrome (MetS) and two latest optimised indices, waist divided by height (WHT.5R) and body mass index (BMI) multiply by the square root of BMI and waist circumference (BMI√WC), and to comprehensively compare the ability of 10 anthropometric indices to identify MetS, including BMI√WC, WHT.5R, abdominal volume index (AVI), waist-to-height ratio (WHtR), Body Roundness Index (BRI).

Design: Cross-sectional study.

Setting: Ningxia, China.

Participants: A total of 3860 adults aged 18-88 years from Ningxia, China were recruited by a single stratified cluster random sampling method.

Methods: ORs and 95% CIs for associations between indices and MetS were calculated using binary logistic regression, and areas under the receiver operating characteristic (ROC) curves (AUCs) were performed to compare their predictive capacity for MetS. All results for men and women were analysed and presented separately.

Results: The highest adjusted ORs for MetS were observed for the following indices: AVI (OR 15.22, 95% CI 10.88 to 21.30), WHT.5R (OR 13.83, 95% CI 9.89 to 19.35) and BMI√WC (OR 12.14, 95% CI 8.79 to 16.76) in men, whereas BRI, WHtR (both OR 14.91, 95% CI 10.39 to 21.4), WHT.5R (OR 14.22, 95% CI 9.96 to 20.29) and AVI (OR 14.03, 95% CI 9.91 to 19.87) in women. In ROC analysis, AVI (AUC: 0.767 for men and 0.749 for women) and WHT.5R (AUC: 0.761 for men and 0.747 for women) showed the highest predictive ability for MetS.

Conclusions: BMI√WC and WHT.5R were significantly positively associated with MetS, and AVI and WHT.5R could be useful screening tools for identifying MetS in both sexes.
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http://dx.doi.org/10.1136/bmjopen-2020-043952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853002PMC
January 2021

Modeling Multidimensional Public Opinion Polarization Process under the Context of Derived Topics.

Int J Environ Res Public Health 2021 01 8;18(2). Epub 2021 Jan 8.

School of Tourism and Urban-Rural Planning, Zhejiang Gongshang University, Hangzhou 310018, China.

With the development of Internet technology, the speed of information dissemination and accelerated updates result in frequent discussion of topics and expressions of public opinion. In general, multi-dimensional discussion topics related to the same event are often generated in the network, and the phenomenon of multi-dimensional public opinion polarization is formed under the mutual influence of groups. This paper targets the phenomenon of multi-dimensional public opinion polarization under topic-derived situations as the research object. Firstly, this paper identifies the factors influencing multi-dimensional public opinion polarization, including the mutual influence of different topic dimensions and the interaction of viewpoints within the same topic. Secondly, the topic correlation coefficient is introduced to describe the correlation among topics in different dimensions, and the individual topic support degree is used to measure the influence of topics in different dimensions and that of information from external intervention on individual attitudes. Thirdly, a multi-dimensional public opinion polarization model is constructed by further integrating multi-dimensional attitude interaction rules. Finally, the influence of individual participation, topic status, topic correlation coefficient and external intervention information on the multi-dimensional public opinion polarization process is analyzed through simulation experiments. The simulation results show that:(1) when there is a negative correlation between multi-dimensional topics, as the number of participants on different dimensional topics becomes more consistent, the conflict between multi-dimensional topics will weaken the polarization effect of overall public opinion. However, the effect of public opinion polarization will be enhanced alongwith the enhancement in the confidence of individual opinions. (2) The intervention of external intervention information in different dimensions at different times will further form a multi-dimensional and multi-stage public opinion polarization, and when the multi-dimensional topics are negatively correlated, the intervention of external intervention information will have a stronger impact on the multi-dimensional and multi-stage public opinion polarization process. Finally, the rationality and validity of the proposed model are verified by a real case.
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http://dx.doi.org/10.3390/ijerph18020472DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826538PMC
January 2021

A comparative study of the disease burden attributable to PM in China, Japan and South Korea from 1990 to 2017.

Ecotoxicol Environ Saf 2021 Feb 4;209:111856. Epub 2021 Jan 4.

Department of Environmental Health, Boston University School of Public Health, Boston, MA, USA. Electronic address:

Background: Exposure to fine particulate matter (PM) is one of the leading contributors to disease burden. However, little is known about the spatial and temporal trends of the disease burden attributable to PM in the three major economies in East Asia. We aimed to estimate the patterns and temporal variations of the disease burden attributable to PM in China, Japan, and South Korea from 1990 to 2017.

Methods: We obtained data on disease burden attributable to PM from the Global Burden of Disease Study (GBD) 2017. We retrieved the numbers and age-standardized mortality rate (ASMR) and disability-adjusted life years (DALYs) rate (ASDR) of disease attributable to PM by age, sex, socio-demographic index (SDI), and country. We used percentage change and estimated annual percentage change (EAPC) to assess the trends of ASMR and ASDR attributable to PM between 1990 and 2017. We further calculated the contribution of population growth, population aging, and changes in mortality or DALYs rate to the net changes in total deaths and DALYs associated with PM.

Results: We found considerable differences in the disease burden attributable to PM in China, Japan, and South Korea. In 2017, the ASMR and ASDR of disease attributable to PM in China were 49.37 (95% UI: 41.18, 57.5) per 100,000 population and 1065.9 (95% UI: 891.28, 1237.38) per 100,000 population, respectively, which was about four times higher than that of Japan and twice higher than that of South Korea. Regardless of country, the ASMR and ASDR were more pronounced among elders and males. From 1990 to 2017, the declines in ASMR and ASDR were more pronounced in Japan and South Korea than in China. The changes in PM associated total deaths and DALYs between 1990 and 2017 were the combined effects of population aging, population growth, and changes in mortality or DALY rate, resulting in a net increase in total deaths and DALYs in China but little changes in Japan and South Korea.

Conclusions: PM still contributed to significant disease burdens in 2017, although age-standardized disease burden has declined from 1990 to 2017. There has been an increasing trend in total deaths and DALYs in China, which was primarily driven by population aging.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111856DOI Listing
February 2021

A Policy Category Analysis Model for Tourism Promotion in China During the COVID-19 Pandemic Based on Data Mining and Binary Regression.

Risk Manag Healthc Policy 2020 31;13:3211-3233. Epub 2020 Dec 31.

Hubei Key Laboratory of Mechanical Transmission and Manufacturing Engineering, Wuhan University of Science and Technology, Wuhan 430081, People's Republic of China.

Background And Aim: At the end of 2019, the outbreak of COVID-19 had a significant impact on China's tourism industry, which was almost at a standstill in the short-term. After reaching the preliminarily stable state, the government and the scenic area management department implemented a series of incentive policies in order to speed up the recovery of the tourism industry. Therefore, analyzing all sorts of social effects after policy implementation is of guiding significance for the government and the scenic areas.

Methods: Targeted as the social effect with the implementation of tourism promotion policy during the COVID-19 pandemic, this paper briefly analyzes the impact of COVID-19 on the national cultural and tourism industry and selects several representative types of tourism policies, crawls the comment data of Weibo users, analyzes users' perception and emotional preference to the policy, and thus mines the social effect of various policies. Subsequently, by identifying the social effects of various policies as dependent variables, a binary logistic regression model is constructed to obtain the best combination of tourism promotion policies and promote the rapid revitalization of the cultural and tourism industry.

Results: The results show that from the single policy, the social effect of the "safety" policy is the best. From the perspective of combination policies, the simultaneous release of "safety" policies and "economy" policies have the greatest social impact, which can dramatically accelerate the recovery of the cultural and tourism industry. Finally, this paper proposes suggestions for policy formulation to improve the ability of the cultural tourism industry to cope with crisis events.

Conclusion: These results explain the perceived effects of the public on the government policies and can be used to judge whether the policies have been released in place. Based on the above results, corresponding suggestions are proposed as follows: 1) the combination of economic policies and security policies can achieve better results; and 2) the role of "opinion leaders" can be played to improve the perceived effect of policies.
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http://dx.doi.org/10.2147/RMHP.S284564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781111PMC
December 2020

Based on Computational Communication Paradigm: Simulation of Public Opinion Communication Process of Panic Buying During the COVID-19 Pandemic.

Psychol Res Behav Manag 2020 20;13:1027-1045. Epub 2020 Nov 20.

School of Tourism and Urban-Rural Planning, Zhejiang Gongshang University, Hangzhou 310018, People's Republic of China.

Background And Aim: The spread of the COVID-19 pandemic has led to a number of instances of large-scale panic buying. Taking the COVID-19 pandemic as an example, this paper explores the impact of panic in uncertain environments on panic buying behavior. Under certain circumstances, the spread of rumors about shortage of goods is likely to cause large-scale panic buying. This paper focuses on the study of such panic buying caused by online rumors.

Methods: Firstly, based on the improved BA network, this paper constructs a directed network for public opinion communication and integrates an offline communication network to build a two-layer synchronous coupling network based on online and offline communications. Secondly, the individual decision model and the panic emotion transmission model under the uncertain environment are constructed. Netizens judge the authenticity of network information, determine their own panic degree according to the above two models, and judge whether they participate in the panic buying based on the above factors. Finally, the spread of the public opinion of goods buying under the panic state is simulated and analyzed.

Results: The experimental results of the two-layer synchronous network that integrates offline interaction are significantly different from the results of pure online interaction, which increases the speed of public opinions spread after offline interaction and affects a wider range of groups. Under the condition of sufficient supplies, panic in local areas will not cause large-scale panic buying on the whole network. However, the results under the same parameters suggest that if there is a shortage of supplies, panic will spread quickly across the network, leading to large-scale panic buying. It is very important to ensure sufficient supply of materials at the beginning of the spread of rumors, which can reduce the number of buyers. However, if there is a shortage of goods before the panic dissipates in the later stage, there will still be a large-scale rush purchase.

Conclusion: These results explain the reasons why it is difficult to stop the buying events in many areas under the COVID-19 pandemic. Under the uncertain environment, the panic caused by people's fear of stock shortage promotes the occurrence of large-scale rush buying. Therefore, in the event of major public health events, ensuring adequate supply of materials is the top priority.
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http://dx.doi.org/10.2147/PRBM.S280825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691764PMC
November 2020

The effect of femtosecond laser fluence and pitches between V-shaped microgrooves on the dynamics of capillary flow.

Results Phys 2020 Dec;19:103606

The Photonics Laboratory, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China.

Open microgroove is one kind of capillary-driven superwicking surface structure. In this study, arrays of parallel V-shaped microgrooves were fabricated on an aluminum surface by using a femtosecond laser to obtain a superwicking surface which can quickly transport the water uphill against gravity. The relationships between the flowing time and flowing distance were investigated and compared with theoretical results. We demonstrated both laser fluence and scanning step size can affect the superwicking performance. The aluminum surfaces fabricated at a laser fluence of 18.49 J/cm and 52.67 J/cm showed the best superwicking performances with the average water flow velocities approximately 16.2 mm/s and 16.4 mm/s, respectively, in the distance of 30 mm. On the other hand, the superwicking surfaces show an anisotropic flow characteristic due to the parallel microgrooves structure. However, when the scanning step size drops to 25 µm, the surface will form irregular rough structures that result in the isotropic flow characteristics. Moreover, by using a thermal camera, we found that after a 10 µL water droplet was dropped into the heated surface, the superwicking surface temperature quickly dropped from 92.4 °C to 82.5 °C which indicated that laser processing of the superwicking surface has potential application in heat dissipation.
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http://dx.doi.org/10.1016/j.rinp.2020.103606DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762841PMC
December 2020

Integrase-Defective Lentiviral Vectors for Delivery of Monoclonal Antibodies against Influenza.

Viruses 2020 12 17;12(12). Epub 2020 Dec 17.

Department of Medicine, Weill Cornell Medical College, New York, NY 10021, USA.

Delivering rapid protection against infectious agents to non-immune populations is a formidable public health challenge. Although passive immunotherapy is a fast and effective method of protection, large-scale production and administration of monoclonal antibodies (mAbs) is expensive and unpractical. Viral vector-mediated delivery of mAbs offers an attractive alternative to their direct injection. Integrase-defective lentiviral vectors (IDLV) are advantageous for this purpose due to the absence of pre-existing anti-vector immunity and the safety features of non-integration and non-replication. We engineered IDLV to produce the humanized mAb VN04-2 (IDLV-VN04-2), which is broadly neutralizing against H5 influenza A virus (IAV), and tested the vectors' ability to produce antibodies and protect from IAV in vivo. We found that IDLV-transduced cells produced functional VN04-2 mAbs in a time- and dose-dependent fashion. These mAbs specifically bind the hemagglutinin (HA), but not the nucleoprotein (NP) of IAV. VN04-2 mAbs were detected in the serum of mice at different times after intranasal (i.n.) or intramuscular (i.m.) administration of IDLV-VN04-2. Administration of IDLV-VN04-2 by the i.n. route provided rapid protection against lethal IAV challenge, although the protection did not persist at later time points. Our data suggest that administration of mAb-expressing IDLV may represent an effective strategy for rapid protection against infectious diseases.
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http://dx.doi.org/10.3390/v12121460DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7767071PMC
December 2020