Publications by authors named "Jianjun Wang"

678 Publications

A Synchronous Magnitude Estimation with P-Wave Phases' Detection Used in Earthquake Early Warning System.

Sensors (Basel) 2022 Jun 16;22(12). Epub 2022 Jun 16.

National Institute of Natural Hazards, Ministry of Emergency Management of China, Beijing 100085, China.

How to estimate an earthquake's magnitude rapidly and accurately is a challenge for any earthquake early warning system. In order to reach a balance between accuracy and timeliness, a synchronous magnitude estimation method with P-wave phases' detection is proposed. In this method, the P-wave phases are detected by the changes of the signal-to-noise ratio (SNR) of the seismic records, where the SNRs are calculated by the short-term power and long-term power ratio (STP/LTP). Meanwhile, the variations of the SNR are applied to estimate the magnitude of the earthquake. By the statistics of some earthquake cases, a synchronous magnitude estimation model of the variation of the P-wave phases' SNR, the earthquake magnitude, and the hypocentral distance was built. Compared with some other magnitude estimation methods, the suggested method inherits the robustness of the STP/LTP method and is more accurate and rapid than the peak displacement (Pd) method.
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http://dx.doi.org/10.3390/s22124534DOI Listing
June 2022

Comparative Transcriptome Analysis of Purple and Green Non-Heading Chinese Cabbage and Function Analyses of Gene.

Genes (Basel) 2022 May 31;13(6). Epub 2022 May 31.

State Key Laboratory of Crop Genetics & Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture and Rural Affairs of the P. R. China, Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education of the P. R. China, Nanjing Agricultural University, Nanjing 210095, China.

Non-heading Chinese cabbage ( ssp. ) is an important vegetative crop in the south of China. As an antioxidant, anthocyanin is the major quality trait for vegetables with purple leaves or petioles. However, the molecular biosynthetic mechanism of anthocyanin in non-heading Chinese cabbage has not been explained exclusively. In this study, two non-heading Chinese cabbage with contrasting colors in the leaves were used as the materials for RNA-seq. A total of 906 DEGs were detected, and we found that the anthocyanin and flavonoid biosynthetic pathways are significantly enriched in the purple NHCC. The transcriptome result was verified by RT-qPCR. Though bioinformatics analysis, was selected as the candidate gene for the regulation of anthocyanin synthesis, and the characterization of was elucidated by the functional analyses. The results proved that BcTT8 is a nucleus protein and phylogenetically close to the TT8 protein from . After silencing , the total anthocyanin content of pTY- plants decreased by 42.5%, and the relative expression levels of anthocyanin pathway genes , and were significantly downregulated, while the transcription level of was significantly upregulated. Compared with the wild type, the transgenic showed obvious violet in the cotyledons part, and the anthocyanin biosynthetic genes such as and were significantly upregulated. In conclusion, is critical in the anthocyanin synthesis process of non-heading Chinese cabbage. Our findings illustrated the molecular mechanism of anthocyanin biosynthesis in non-heading Chinese cabbage.
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http://dx.doi.org/10.3390/genes13060988DOI Listing
May 2022

circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop.

Mol Cancer 2022 Jun 23;21(1):135. Epub 2022 Jun 23.

Guangdong Province Key Laboratory of Molecular Tumor Pathology, Department of Pathology, Southern Medical University, Guangzhou, 510515, Guangdong Province, China.

Background: In recent years, an increasing number of studies have indicated that circular RNA plays crucial roles in regulating tumor development and chemoresistance. Using two high-throughput RNA sequence datasets, we previously found that circEXOC6B was downregulated in colon cancer. However, its role and mechanism in colorectal cancer (CRC) remained unknown.

Methods: Real-time quantitative PCR was used to examine the expression of circEXOC6B in CRC tissues. In vivo and in vitro functional experiments were performed to determine the suppressor role of circEXOC6B in CRC progression. RNA pull-down, mass spectrometry, RNA-binding protein immunoprecipitation, co-immunoprecipitation, fluorescence in situ hybridization, and immunofluorescence were applied to investigate the possible mechanisms connecting circEXOC6B to CRC growth and 5-fluorouracil-induced apoptosis. Chromatin immunoprecipitation, dual-luciferase assay, western blot, and immunohistochemistry were used to explore the mechanisms underlying the HIF1A regulation of RRAGB transcription.

Results: circEXOC6B was downregulated in CRC tissues, and its lower expression was associated with poor prognosis of patients. Functional experiments showed that circEXOC6B inhibited growth and increased the 5-fluorouracil-induced apoptosis of CRC cells in vitro and in vivo. Mechanistically, circEXOC6B inhibited the heterodimer formation of RRAGB by binding to it, thereby suppressing the mTORC1 pathway and HIF1A level. In addition, HIF1A upregulated the transcription of RRAGB by binding to its promoter region. Altogether, the results demonstrated that a HIF1A-RRAGB-mTORC1 positive feedback loop drives tumor progression in CRC, which could be interrupted by circEXOC6B.

Conclusions: circEXOC6B inhibits the progression of CRC and enhances the chemosensitivity of CRC cells to 5-fluorouracil by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop. circEXOC6B is a possible therapeutic target for CRC treatment.
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http://dx.doi.org/10.1186/s12943-022-01600-1DOI Listing
June 2022

Ecological networks of dissolved organic matter and microorganisms under global change.

Nat Commun 2022 Jun 23;13(1):3600. Epub 2022 Jun 23.

State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academic of Sciences, Nanjing, 210008, China.

Microbes regulate the composition and turnover of organic matter. Here we developed a framework called Energy-Diversity-Trait integrative Analysis to quantify how dissolved organic matter and microbes interact along global change drivers of temperature and nutrient enrichment. Negative and positive interactions suggest decomposition and production processes of organic matter, respectively. We applied this framework to manipulative field experiments on mountainsides in subarctic and subtropical climates. In both climates, negative interactions of bipartite networks were more specialized than positive interactions, showing fewer interactions between chemical molecules and bacterial taxa. Nutrient enrichment promoted specialization of positive interactions, but decreased specialization of negative interactions, indicating that organic matter was more vulnerable to decomposition by a greater range of bacteria, particularly at warmer temperatures in the subtropical climate. These two global change drivers influenced specialization of negative interactions most strongly via molecular traits, while molecular traits and bacterial diversity similarly affected specialization of positive interactions.
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http://dx.doi.org/10.1038/s41467-022-31251-1DOI Listing
June 2022

Microbial and Environmental Processes Shape the Link between Organic Matter Functional Traits and Composition.

Environ Sci Technol 2022 Jun 23. Epub 2022 Jun 23.

State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academic of Sciences, Nanjing 210008, China.

Dissolved organic matter (DOM) is a large and complex mixture of molecules that fuels microbial metabolism and regulates biogeochemical cycles. Individual DOM molecules have unique functional traits, but how their assemblages vary deterministically under global change remains poorly understood. Here, we examine DOM and associated bacteria in 300 aquatic microcosms deployed on mountainsides that span contrasting temperatures and nutrient gradients. Based on molecular trait dimensions of reactivity and activity, we partition the DOM composition into labile-active, recalcitrant-active, recalcitrant-inactive, and labile-inactive fractions and quantify the relative influences of deterministic and stochastic processes governing the assembly of each. At both subtropical and subarctic study sites, the assembly of labile or recalcitrant molecules in active fractions is primarily governed by deterministic processes, while stochastic processes are more important for the assembly of molecules within inactive fractions. Surprisingly, the importance of deterministic selection increases with global change gradients for recalcitrant molecules in both active and inactive fractions, and this trend is paralleled by changes in the deterministic assembly of microbial communities and environmental filtering, respectively. Together, our results highlight the shift in focus from potential reactivity to realized activity and indicate that active and inactive fractions of DOM assemblages are structured by contrasting processes, and their recalcitrant components are consistently sensitive to global change. Our study partitions the DOM molecular composition across functional traits and links DOM with microbes via a shared ecological framework of assembly processes. This integrated approach opens new avenues to understand the assembly and turnover of organic carbon in a changing world.
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http://dx.doi.org/10.1021/acs.est.2c01432DOI Listing
June 2022

Inorganic crosslinked supramolecular binder with fast Self-Healing for high performance silicon based anodes in Lithium-Ion batteries.

J Colloid Interface Sci 2022 Jun 6;625:373-382. Epub 2022 Jun 6.

Beijing Key Laboratory of Energy Conversion and Storage Materials, College of Chemistry, Beijing Normal University, Beijing 100875, China. Electronic address:

Capacity retention is one of the key factors affecting the performance of silicon (Si)-based lithium-ion batteries and other energy storage devices. Herein, a three dimension (3D) network self-healing binder (denoted as PVA + LB) consisting of polyvinyl alcohol (PVA) and lithium metaborate (LiBO) solution is proposed to improve the cycle stability of Si-based lithium-ion batteries. The reversible capacity of the silicon electrode is maintained at 1767.3 mAh g after 180 cycles when employing PVA + LB as the binder, exhibiting excellent cycling stability. In addition, the silicon/carbon (Si/C) anode with the PVA + LB binder presents superior electrochemical performance, achieving a stable cycle life with a capacity retention of 73.7% (858.3 mAh g) after 800 cycles at a current density of 1 A g. The high viscosity and flexibility, 3D network structure, and self-healing characteristics of the PVA + LB binder are the main reasons to improve the stability of the Si or Si/C contained electrodes. The novel self-healing binder shows great potential in designing the new generation of silicon-based lithium-ion batteries and even electrochemical energy storage devices.
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http://dx.doi.org/10.1016/j.jcis.2022.06.002DOI Listing
June 2022

Effects of Plantation Type and Soil Depth on Microbial Community Structure and Nutrient Cycling Function.

Front Microbiol 2022 31;13:846468. Epub 2022 May 31.

Liaoning Academy of Forestry Sciences, Shenyang, China.

Declining soil quality and microecological imbalances were evaluated in larch plantations in this study. One potential solution to this problem is the cultivation of mixed coniferous and broad-leaved plantations. However, it is unclear whether and how soil microbial community structure and nutrient cycling function would be affected by mixed plantations and soil depths. In this study, we used high-throughput sequencing technology to investigate bacterial 16S and fungal ITS regions for comparisons of soil microbial diversity among plantation types (a pure plantation, a pure plantation, a - mixed plantation within the row, the row, and between the and rows) and soil depths (0-10, 10-20, and 20-40 cm). These data were used to evaluate variations in microbial communities and nutrient cycling function with the determining environmental factors. Our results indicated that bacteria had a stronger spatial dependence than did fungi, while plantation types significantly affected the fungal community. The relative abundance of , as well as bacterial ligninolysis, nitrate ammonification, and nitrite ammonification functions significantly increased with increasing soil depth. Compared with other plantations, the relative abundance of was significantly higher in the plantation. Distance-based redundancy analysis (db-RDA) showed that and abundances were positively correlated with ammonium nitrogen content, available phosphorus content, and phosphatase activity. Our findings indicate that variations in soil available phosphorus are closely related to the relative abundances of at different soil depths and in different plantation types. Mixed plantations might change the availability of soil phosphorus by controlling the relative abundance of . We recommend that fungal community changes be considered in the sustainable management of mixed plantations.
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http://dx.doi.org/10.3389/fmicb.2022.846468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197460PMC
May 2022

An Examination of Threshold Setting in Social Emotional Measurement.

Authors:
Jianjun Wang

J Nurs Meas 2022 Jun 15. Epub 2022 Jun 15.

California State University, Bakersfield, 9001 Stockdale Highway, Bakersfield, CA 93311

Background And Purpose: Thresholds are used by nurses in social emotional screening. A purpose of this study is to examine fluctuation of the threshold pattern in a widely-used instrument, Ages and Stages Questionnaire-Social Emotional, Second Edition (ASQ:SE-2).

Methods: Threshold settings are investigated against an irreversible pattern of child growth from research literature. Empirical studies are conducted on an issue of 6,039 missing cases in the ASQ:SE-2 normative sample that undermines data quality for the cutoff score configuration.

Results: A Bayesian estimate has been suggested to improve the threshold at age 3 with an asymptotically unbiased cutoff score. Given the coverage of ASQ:SE-2 for children from less than 6 months to 72 months for mental health referrals, this study is particularly relevant to the practice of social emotional screening performed by pediatric nurses, school nurses, and nurse practitioners at mental health hospitals.

Conclusion: To address the violation of Classical Test Theory and Item Response Theory in the ASQ:SE-2 threshold settings, consistent statistical imputations are needed to maintain monotonicity of the cutoff score patterns that are aligned with the irreversible trend of child growth, as well as the well-established national standards for missing data examination.
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http://dx.doi.org/10.1891/JNM-2021-0023DOI Listing
June 2022

Growth rate determines prokaryote-provirus network modulated by temperature and host genetic traits.

Microbiome 2022 Jun 14;10(1):92. Epub 2022 Jun 14.

Key Laboratory of Biometallurgy of Ministry of Education, School of Minerals Processing and Bioengineering, Central South University, Changsha, 410006, China.

Background: Prokaryote-virus interactions play key roles in driving biogeochemical cycles. However, little is known about the drivers shaping their interaction network structures, especially from the host features. Here, we compiled 7656 species-level genomes in 39 prokaryotic phyla across environments globally and explored how their interaction specialization is constrained by host life history traits, such as growth rate.

Results: We first reported that host growth rate indicated by the reverse of minimal doubling time was negatively related to interaction specialization for host in host-provirus network across various ecosystems and taxonomy groups. Such a negative linear growth rate-specialization relationship (GrSR) was dependent on host optimal growth temperature (OGT), and stronger toward the two gradient ends of OGT. For instance, prokaryotic species with an OGT ≥ 40 °C showed a stronger GrSR (Pearson's r = -0.525, P < 0.001). Significant GrSRs were observed with the presences of host genes in promoting the infection cycle at stages of adsorption, establishment, and viral release, but nonsignificant with the presence of immune systems, such as restriction-modification systems and CRISPR-Cas systems. Moreover, GrSR strength was increased with the presence of temperature-dependent lytic switches, which was also confirmed by mathematical modeling.

Conclusions: Together, our results advance our understanding of the interactions between prokaryotes and proviruses and highlight the importance of host growth rate in interaction specialization during lysogenization. Video Abstract.
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http://dx.doi.org/10.1186/s40168-022-01288-xDOI Listing
June 2022

Accelerated biocorrosion of stainless steel in marine water via extracellular electron transfer encoding gene phzH of Pseudomonas aeruginosa.

Water Res 2022 Jul 18;220:118634. Epub 2022 May 18.

Shenyang National Laboratory for Materials Science, Northeastern University, Shenyang, 110819, China; Electrobiomaterials Institute, Key Laboratory for Anisotropy and Texture of Materials (Ministry of Education), Northeastern University, Shenyang, 110819, China.

Microbiologically influenced corrosion (MIC) constantly occurs in water/wastewater systems, especially in marine water. MIC contributes to billions of dollars in damage to marine industry each year, yet the physiological mechanisms behind this process remain poorly understood. Pseudomonas aeruginosa is a representative marine electro-active bacterium, which has been confirmed to cause severe MIC on carbon steel through extracellular electron transfer (EET). However, little is known about how P. aeruginosa causes corrosion on stainless steel. In this study, the corrosivity of wild-type strain, phzH knockout, phzH complemented, and phzH overexpression P. aeruginosa mutants were evaluated to explore the underlying MIC mechanism. We found the accelerated MIC on 2205 duplex stainless steel (DSS) was due to the secretion of phenazine-1-carboxamide (PCN), which was regulated by the phzH gene. Surface analysis, Mott-Schottky test and HO measurement results showed that PCN damaged the passive film by forming HO to oxidize chromium oxide to soluble hexavalent chromium, leading to more severe pitting corrosion. The normalized corrosion rate per cell followed the same order as the general corrosion rate obtained under each experimental condition, eliminating the influence of the total amount of sessile cells on corrosion. These findings provide new insight and are meaningful for the investigation of MIC mechanisms on stainless steel. The understanding of MIC can improve the sustainability and resilience of infrastructure, leading to huge environmental and economic benefits.
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http://dx.doi.org/10.1016/j.watres.2022.118634DOI Listing
July 2022

Sediment organic matter properties facilitate understanding nitrogen transformation potentials in East African lakes.

Sci Total Environ 2022 Jun 9;841:156607. Epub 2022 Jun 9.

State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China. Electronic address:

East African lakes include the most productive and alkaline lake group in the world. Yet, they generally receive fewer nutrient inputs than the densely populated subtropical and temperate lakes in the northern hemisphere. In these lakes with insufficient supplies of inorganic nitrogen, the mineralization of benthic organic matter can play an important role in driving the nutrient cycle and nitrogen loss. Using a suite of stable N isotope dilution and tracer techniques, we examined five main processes of the sediment nitrogen cycle in 16 lakes and reservoirs of Tanzania and Kenya, East Africa: gross nitrogen mineralization, ammonium immobilization, dissimilatory nitrate reduction to ammonium (DNRA), and the dinitrogen (N) production via denitrification and anaerobic ammonium oxidation (anammox). Gross nitrogen mineralization and ammonium immobilization showed the maximum values of 9.84 and 12.39 μmol N kg h, respectively. Potential DNRA rates ranged from 0.22 to 8.15 μmol N kg h and accounted for 10 %-74 % (average 25 %) of the total dissimilatory nitrate reduction. Potential nitrate reduction rates in most lakes were dominated by denitrification with a contribution of 26 %-85 % and a mean of 65 %. We further found that the sediment nitrogen transformations were driven mainly by benthic organic matter properties and water column phosphate concentrations, reflecting microbial metabolic responses to the changing carbon and nutrients availability. For instance, autochthonous production of protein-like organic matter attributed to active sediment nitrogen mineralization, DNRA, and denitrification. In contrast, the high degree of humification caused by the inputs of terrestrial humic-like substances slowed down the sediment nitrogen transformations. The contribution of DNRA to total dissimilatory nitrate reduction was significantly positively correlated to sediment C: N ratios. These results indicate that predictions of sediment N supply and loss in East African lakes can be improved by incorporating sediment organic matter properties.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156607DOI Listing
June 2022

S6K1 acts through FOXO to regulate juvenile hormone biosynthesis in the red flour beetle, Tribolium castaneum.

J Insect Physiol 2022 Jun 6;140:104405. Epub 2022 Jun 6.

College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou 225009, China. Electronic address:

As the downstream effector of the target of rapamycin complex 1 (TORC1) signaling pathway, the ribosomal protein S6 kinase (S6K) is an important regulator of insect reproduction, however, the underlying mechanism remains obscure. In this study, a S6K gene, named TcS6K1, was isolated from the red flour beetle, Tribolium castaneum. Analysis of temporal and spatial expression patterns revealed that TcS6K1 is expressed at the highest level in the one-day-old first instar larvae and head of 7-day-old females, respectively. RNAi-mediated knockdown of TcS6K1 in either female or male adults decreased the number of eggs laid, with a concomitant reduction of mRNA levelsof vitellogenin genes, TcVg1 and TcVg2, two male accessory gland secretory proteins, as well as the juvenile hormone (JH) biosynthesis-related gene, farnesol dehydrogenase (TcFDH). While the mRNA and protein levels of the transcription factor forkhead box O (TcFOXO) were not affected, suppression of TcS6K1 expression promoted TcFOXO nuclear translocation to exert its transcriptional action. Further RNAi and EMSA analysis revealed that TcFOXO negatively regulated the expression of TcFDH. These results indicate that S6K might regulate beetles' reproduction through FOXO/JH signaling pathway.
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http://dx.doi.org/10.1016/j.jinsphys.2022.104405DOI Listing
June 2022

β-aminobutyric acid (BABA)-induced resistance to tobacco black shank in tobacco (Nicotiana tabacum L.).

PLoS One 2022 9;17(6):e0267960. Epub 2022 Jun 9.

Yunnan Tobacco Co., Ltd., Kunming Branch, Kunming, China.

Tobacco black shank is a kind of soil-borne disease caused by the Oomycete Phytophthora parasitica. This disease is one of the most destructive diseases to tobacco (Nicotiana tabacum L.) growth worldwide. At present, various measures have been taken to control this disease, but they still have different challenges and limitations. Studies have shown that β-aminobutyric acid (BABA), a nonprotein amino acid, can enhance disease resistance in plants against different varieties of pathogens. However, it is unclear whether BABA can induce plants to resist Phytophthora parasitica infection. Therefore, this study aims to explore the effect and related mechanism of BABA against tobacco black shank. Our results showed that 5 mmol.L-1 BABA had an obvious anti-inducing effect on the pathogenic fungus and could effectively inhibit the formation of dark spots in the stems. The results also showed that a large amount of callose deposition was observed in BABA-treated tobacco. Furthermore, the application of BABA induced the accumulation of H2O2 in tobacco and effectively regulated the homeostasis of reactive oxygen in tobacco plants, reducing the toxicity of H2O2 to plants while activating the defense system. In addition, BABA spray treatment could induce an increase in the concentrations of salicylic acid (SA) and jasmonic acid-isoleucine (JA-Ile) in tobacco, and the gene expression results confirmed that BABA upregulated the expression of SA-related genes (PR1, PR2 and PR5), JA-related genes (PDF1.2) and ET-related genes (EFE26 and ACC oxidase) in tobacco plants. Taken together, BABA could activate tobacco resistance to black shank disease by increasing H2O2 accumulation, callose deposition, plant hormone (SA and JA-Ile) production, and SA-, JA-, and ET- signaling pathways.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0267960PLOS
June 2022

Small-molecule fulvic acid with strong hydration ability for non-vitreous cellular cryopreservation.

iScience 2022 Jun 18;25(6):104423. Epub 2022 May 18.

Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

The exploitation of biocompatible ice-control materials especially the small molecules for non-vitreous cryopreservation remains challenging. Here, we report a small molecule of fulvic acid (FA) with strong hydration ability, which enables non-vitreous cellular cryopreservation by reducing ice growth during freezing and reducing ice recrystallization/promoting ice melting during thawing. Without adding any other cryoprotectants, FA can enhance the recovery of sheep red blood cells (RBCs) by three times as compared with a commercial cryoprotectant (hydroxyethyl starch) under a stringent test condition. Investigation of water mobility reveals that the ice-control properties of FA can be ascribed to its strong bondage to water molecules. Furthermore, we found that FA can be absorbed by RBCs and mainly locates on membranes, suggesting the possible contribution of FA to cell protection through stabilizing membranes. This work bespeaks a bright future for small-molecule cryoprotectants in non-vitreous cryopreservation application.
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http://dx.doi.org/10.1016/j.isci.2022.104423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157229PMC
June 2022

Virally Mediated Connexin 26 Expression in Postnatal Scala Media Significantly and Transiently Preserves Hearing in Connexin 30 Null Mice.

Front Cell Dev Biol 2022 27;10:900416. Epub 2022 Apr 27.

Department of Otolaryngology, Emory University School of Medicine, Atlanta, GA, United States.

Non-sensory cells in the sensory epithelium of the cochlea are connected extensively by gap junctions. Functionally null mutations in (encoding Cx30) cause hearing loss in humans. In this study, we injected AAV1-CB7- into the scala media between P0-2 in the cochlea of mice. The injection increased Cx26 expression and significantly preserved auditory functions. However, the hearing preservation gradually declined and essentially disappeared 3 months after the injections. In contrast, the morphological preservation was still significant at 3 months post-injection. We found that the expression of Cx26, at both the mRNA and protein levels, showed substantial decreases during the 3-month period. Curiously, treatments by injecting AAV1-CB7- with the identical approach failed to yield any hearing preservation. Our results demonstrated the first successful cochlear gene therapy treatment in mouse models by virally expressing a companion gene of .
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http://dx.doi.org/10.3389/fcell.2022.900416DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091169PMC
April 2022

Direct microbial electron uptake as a mechanism for stainless steel corrosion in aerobic environments.

Water Res 2022 Jul 5;219:118553. Epub 2022 May 5.

Electrobiomaterials Institute, Key Laboratory for Anisotropy and Texture of Materials (Ministry of Education), Northeastern University, Shenyang, 110819, China.

Shewanella oneidensis MR-1 is an attractive model microbe for elucidating the biofilm-metal interactions that contribute to the billions of dollars in corrosion damage to industrial applications each year. Multiple mechanisms for S. oneidensis-enhanced corrosion have been proposed, but none of these mechanisms have previously been rigorously investigated with methods that rule out alternative routes for electron transfer. We found that S. oneidensis grown under aerobic conditions formed thick biofilms (∼50 µm) on stainless steel coupons, accelerating corrosion over sterile controls. H and flavins were ruled out as intermediary electron carriers because stainless steel did not reduce riboflavin and previous studies have demonstrated stainless does not generate H. Strain ∆mtrCBA, in which the genes for the most abundant porin-cytochrome conduit in S. oneidensis were deleted, corroded stainless steel substantially less than wild-type in aerobic cultures. Wild-type biofilms readily reduced nitrate with stainless steel as the sole electron donor under anaerobic conditions, but strain ∆mtrCBA did not. These results demonstrate that S. oneidensis can directly consume electrons from iron-containing metals and illustrate how direct metal-to-microbe electron transfer can be an important route for corrosion, even in aerobic environments.
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http://dx.doi.org/10.1016/j.watres.2022.118553DOI Listing
July 2022

Association between the frequency of tooth brushing and esophageal carcinoma risk: an update systematic review and meta-analysis.

J Gastrointest Oncol 2022 Apr;13(2):499-509

Department of Thoracic Surgery, Henan Provincial People's Hospital/People's Hospital of Zhengzhou University/School of Clinical Medicine, Henan University, Zhengzhou, China.

Background: Lower frequency of tooth brushing was thought to be associated with esophageal carcinoma (EC). However, some researchers suggested that this association did not exist or had not yet reached statistical significance. The purpose of this study was to calculate a more precise estimation of the relationship between the frequency of tooth brushing and the risk of EC by combining the results between different studies using the meta-analysis.

Methods: We searched the PubMed, Embase, Web of Science, and Scopus electronic databases up to July 2021. According to PECO approach (Population, Exposure, Comparator and Outcomes), we assessed the association between tooth brushing frequency and EC risk which reported the adjusted risk ratios (adjRR), hazard ratios (adjHR), or odds ratios (adjOR) with 95% confidence interval (CI). The random effects model was used to quantitatively evaluate the combined results. Two researchers independently evaluated the risk bias of the included studies using the Newcastle-Ottawa Scale (NOS). The robustness of results was evaluated by subgroup analysis, sensitivity analysis, and publication bias.

Results: In total, we identified 13 articles with 14 case-control studies which included 16,773 participants and 5,673 patients. Pooled results showed the lowest frequency of brushing was significantly associated with an increased risk of EC in comparison to the highest (adjOR: 2.00, 95% CI: 1.61-2.48). There was moderate heterogeneity among included studies (P=0.001, I=61.4%). The original studies included in this meta-analysis were all case-control studies. Study quality was all moderate or above based on NOS score ranges of 6 stars or more.

Conclusions: Available evidence suggests a low frequency of tooth brushing may be an important risk factor for EC. However, higher quality studies should continue to be conducted to investigate the optimal threshold of brushing frequency for the prevention of EC.
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http://dx.doi.org/10.21037/jgo-22-214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086040PMC
April 2022

Built environment impact on the per capita cycling frequency of family--Based on two-level hierarchical linear model.

PLoS One 2022 12;17(5):e0267891. Epub 2022 May 12.

College of Transportation Engineering, Chang'an University, Xi'an, Shaanxi, China.

At present, there is less attention paid to the relationship between the frequency of travel and built environment, especially in households. In this paper, some of the determining factors in the frequency of daily cycling per household were explored based on the data from 2018 Daily Trip Survey in Xianyang, China. Then a two-level linear model was construct to identify the determining factors in the frequency of per capita daily cycling of household. According to the research results, 22.8% of the differences in the per capita cycling frequency of household are due to the differences between communities. In terms of community factors, the densities of road networks and educational facilities delivered a significantly positive impact on the per capita daily cycling frequency of family; on the contrary, the densities of medical facilities, intersections and POI delivered a significantly negative impact. Per capita cycling frequency varies considerably between households. For instance, the number of bicycles owned and the number of school-age children have a significantly positive impact on the per capita daily cycling frequency of family. However, car ownership, household income and occupation composition impose a significantly negative impact. The findings of this study would benefit the transportation engineers and planners who are keen to boost the use of active means of transportation for residents.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0267891PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098036PMC
May 2022

Interface Coordination Stabilizing Reversible Redox of Zinc for High-Performance Zinc-Iodine Batteries.

Small 2022 Jun 6;18(22):e2200168. Epub 2022 May 6.

Key Laboratory for Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China.

Aqueous Zn batteries (AZBs) have attracted extensive attention due to good safety, cost-effectiveness, and environmental benignity. However, the sluggish kinetics of divalent zinc ion and the growth of Zn dendrites severely deteriorate the cycling stability and specific capacity. The authors demonstrate modulation of the interfacial redox process of zinc via the dynamic coordination chemistry of phytic acid with zinc ions. The experimental results and theoretical calculation reveal that the in-situ formation of such inorganic-organic films as a dynamic solid-electrolyte interlayer is efficient to buffer the zinc ion transfer via the energy favorable coordinated hopping mechanism for the reversible zinc redox reactions. Especially, along the interfacial coating layer with porous channel structure is able to regulate the solvation structure of zinc ions along the dynamic coordination of the phytic acid skeleton, efficiently inhibiting the surface corrosion of zinc and dendrite growth. Therefore, the resultant Zn anode achieves low voltage hysteresis and long cycle life at rigorous charge and discharge circulation for fabricating highly robust rechargeable batteries. Such an advanced strategy for modulating ion transport demonstrates a highly promising approach to addressing the basic challenges for zinc-based rechargeable batteries, which can potentially be extended to other aqueous batteries.
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http://dx.doi.org/10.1002/smll.202200168DOI Listing
June 2022

Expression and clinical significance of VISTA and PD-L1 in adrenocortical carcinoma.

Endocr Relat Cancer 2022 May 27;29(7):403-413. Epub 2022 May 27.

Department of Endocrinology, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, China.

Adrenocortical carcinoma (ACC) is a rare malignancy with a poor prognosis and challenging management. The present study aimed to investigate the expression of programmed death ligand-1 (PD-L1) and V-domain Ig-containing suppressor of T cell activation (VISTA) in ACC and their associations with clinicopathological features and survival outcomes. Immunohistochemistry was performed on formalin-fixed paraffin-embedded specimens from 54 ACC patients. Chi-square/Fisher's exact tests or independent samples t/Mann-Whitney U tests were performed to assess correlations between immunoscores and clinicopathological parameters. The Kaplan-Meier method and Cox regression were conducted for survival analysis and to identify independent predictors of overall (OS) and disease-free (DFS) survival. Results showed that VISTA was expressed in tumor cells (TCs) and tumor-infiltrating immune cells (TICs) in 81.5% (44/54) and 40.7% (22/54) of the patients, respectively. PD-L1 positivity was found in either TCs or TICs in 11.1% (6/54) of the patients. Patients with positive VISTA expression in TCs had a higher tumor stage (56.9% vs 20%, P = 0.036) and Ki-67 index (30.50 ± 23.51% vs 14.76 ± 11.75%, P = 0.006). However, PD-L1 positivity in either TCs or TICs had no association with patient clinicopathological features. A higher VISTA expression intensity, a larger area and a higher immunoscore were associated with increased risks of disease progression and overall mortality, but PD-L1 expression in TCs or TICs was not associated with OS or DFS. In conclusion, positive TC VISTA expression was correlated with pathological parameters related to malignancy in ACC patients. This finding provides novel evidence of the value of VISTA, in addition to PD-L1, as an immunotherapeutic target in ACC.
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http://dx.doi.org/10.1530/ERC-22-0066DOI Listing
May 2022

Enrichment of residual carbon from coal gasification fine slag by spiral separator.

J Environ Manage 2022 Aug 29;315:115149. Epub 2022 Apr 29.

School of Chemistry and Chemical Engineering, Xi'an University of Science and Technology, Xi'an, China.

Coal gasification is one of the most promising clean coal technologies. However, gasification process also produces a huge amount of solid waste of high carbon content, named coal gasification fine slag. The coal gasification fine slag is mainly handled by landfilling, which is not only a hazardous pollution, but also wasting the energy from residual carbon. Developing a technology to utilize coal gasification fine slag and recover the residual carbon is becoming essential for an eco-friendly coal chemical industry. In this paper, the enrichment behavior of residual carbon in coal gasification fine slag by a spiral separator is studied. The raw coal gasification fine slag sample and separator products are characterized on particle size distribution, size-depending ash content, reactivity, micromorphology and porous structure. The experimental results show that the spiral separator is efficient to remove ash and enriched carbonaceous components in coal gasification fine slag by separating feed (100%) into concentrate (81.2%), middlings (8.8%), and tailings (10.08%), where the ash content in tailings is up to 90%, accounting for 18.5% of total ash in feeding. The beneficial product "concentrate" has a good distribution of size-depending ash content, that most combustibles are enriched in these particles of diameter >100 μm. After spiral separator, the concentrate products have a more pure and developed porous structure with the surface area increasing from 199.8 m/g (feeding) to 231.8 m/g, as well as a better combustion reactivity of lower ignition temperature compared with feedings. Accordingly, an economic and feasible combination process of spiral separator connecting sieve can produce an enriched-carbon product of ∼45% yield and ∼80% carbonaceous content. The Iodine adsorption ability of sieved products increases by 47.6% compared with feed, and reaches up to about half of industry activated carbon. The finally sieved concentrate products have a good market prospect as fuel and adsorbent.
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http://dx.doi.org/10.1016/j.jenvman.2022.115149DOI Listing
August 2022

Transparent, Photothermal, and Icephobic Surfaces via Layer-by-Layer Assembly.

Adv Sci (Weinh) 2022 May 11;9(14):e2105986. Epub 2022 Mar 11.

Department of Materials Science and Engineering, University of California, Los Angeles, CA, 90095, USA.

Icing and frosting on transparent surfaces compromise visibility on various optical equipment and transparent infrastructures. It remains challenging to fabricate energy-saving coatings for harvesting solar energy while maintaining high transparency. Here, transparent, photothermic, and icephobic composite surfaces composed of photothermal nanomaterials and polyelectrolytes via layer-by-layer assembly are designed and constructed. The positively-charged polypyrrole nanoparticles and negatively-charged poly(acrylic acid) are assembled as exemplary materials via electrostatic attractions. The optically transparent photothermal coatings are successfully fabricated and exhibited photothermal capabilities and light-transmittance performance. Among the various coatings applied, the seven-bilayer coating can increase the temperature by 35 °C under 1.9-sun illumination, maintaining high transmittance (>60%) of visible light. With sunlight illumination at subzero temperatures (> -35 °C), the coatings show pronounced capabilities to inhibit freezing, melt accumulated frost, and decrease ice adhesion. Precisely, the icephobic surfaces remain free of frost at -35 °C as long as sunlight illumination is present; the accumulated frost melts rapidly within 300 s. The ice adhesion strength decreases to ≈0 kPa as the melted water acts as a lubricant. Furthermore, the negatively-charged graphene oxide and positively-charged poly(diallyldimethylammonium chloride) show their material diversity applicable in the coating fabrication.
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http://dx.doi.org/10.1002/advs.202105986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108600PMC
May 2022

Contrasting elevational patterns and underlying drivers of stream bacteria and fungi at the regional scale on the Tibetan Plateau.

FEMS Microbiol Ecol 2022 May;98(6)

State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China.

Elevational gradients are the focus of development and evaluation of general theories on biodiversity. However, elevational studies of microorganisms and the underlying mechanisms remain understudied, especially at regional scales. Here, we examined stream bacterial and fungal communities along an elevational gradient of 990-4600 m with a geographic distance up to 500 km in the southeastern Tibetan Plateau and further analyzed their elevational patterns and drivers of three biodiversity indicators, including species richness, ecological uniqueness, and community composition. Bacteria and fungi showed distinct elevational trends in species richness and consistently decreasing patterns in their ecological uniqueness. The distance-decay relationships were concordant for the two microbial groups, while fungi showed higher dissimilarity and a lower turnover rate. Interestingly, bacterial and fungal compositions substantially differed between the elevations below and above 3000 m. Climate predictors, such as the mean annual temperature and precipitation seasonality, had greater effects than local environment drivers. Notably, fungal diversity was mainly influenced by climate, while bacterial diversity was explained by the shared contributions of climate and local factors. Collectively, these findings revealed the elevational patterns of stream microbial biodiversity across mountains on a large spatial scale and highlight their underlying response mechanisms to environmental predictors.
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http://dx.doi.org/10.1093/femsec/fiac050DOI Listing
May 2022

Abnormal Pulmonary Function in Early Parkinson's Disease: A Preliminary Prospective Observational Study.

Lung 2022 Jun 25;200(3):325-329. Epub 2022 Apr 25.

Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, 710000, China.

Early Parkinson's disease (PD) may cause respiratory dysfunction; however the findings vary among studies. The aim of the preliminary prospective observational study was to explore the deterioration of pulmonary function at various stages in patients with early PD. A total of 237 patients with PD were screened. Fifty-six patients were included (modified Hoehn and Yahr stage ≤ 2.5). In addition, 56 age-matched healthy controls were also included in the study. Significant differences between the PD and control groups were found in all the investigated lung-function parameters. The maximal voluntary ventilation (MVV) percent predicted was the only parameter that distinguished PD stages (101.1 ± 14.9% vs. 82.8 ± 19.2% vs. 71.4 ± 12.9%, Hoehn and Yahr stages 1.5 vs. 2 vs. 2.5, respectively; p < 0.005). MVV could be the most sensitive parameter for distinguishing the severity of early-stage PD.
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http://dx.doi.org/10.1007/s00408-022-00533-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037578PMC
June 2022

The Self-Organization of Marine Microbial Networks under Evolutionary and Ecological Processes: Observations and Modeling.

Biology (Basel) 2022 Apr 13;11(4). Epub 2022 Apr 13.

School of Minerals Processing and Bioengineering, Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha 410083, China.

Evolutionary and ecological processes are primary drivers of ecological network constrictions. However, the ways that these processes underpin self-organization and modularity in networks are poorly understood. Here, we performed network analyses to explore the evolutionary and ecological effects on global marine microbial co-occurrence networks across multiple network levels, including those of nodes, motifs, modules and whole networks. We found that both direct and indirect species interactions were evolutionarily and ecologically constrained across at least four network levels. Compared to ecological processes, evolutionary processes generally showed stronger long-lasting effects on indirect interactions and dominated the network assembly of particle-associated communities in spatially homogeneous environments. Regarding the large network path distance, the contributions of either processes to species interactions generally decrease and almost disappear when network path distance is larger than six. Accordingly, we developed a novel mathematical model based on scale-free networks by considering the joint effects of evolutionary and ecological processes. We simulated the self-organization of microbial co-occurrence networks and found that long-lasting effects increased network stability via decreasing link gain or loss. Overall, these results revealed that evolutionary and ecological processes played key roles in the self-organization and modularization of microbial co-occurrence networks.
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http://dx.doi.org/10.3390/biology11040592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9031791PMC
April 2022

Gut Microbiome Succession in Chinese Mitten Crab During Seawater-Freshwater Migration.

Front Microbiol 2022 30;13:858508. Epub 2022 Mar 30.

Department of Marine Biology, College of Oceanography, Hohai University, Nanjing, China.

Biological migration is usually associated with disturbances and environmental changes that are key drivers in determining the diversity, community compositions, and function of gut microbiome. However, little is known about how gut microbiome is affected by disturbance such as salinity changes during migration from seawater to freshwater. Here, we tracked the gut microbiome succession of Chinese mitten crabs () during their migrations from seawater to freshwater and afterward using 16S rDNA sequencing for 127 days, and explored the temporal patterns in microbial diversity and the underlying environmental factors. The species richness of gut microbiome showed a hump-shaped trend over time during seawater-freshwater migration. The community dissimilarities of gut microbiome increased significantly with day change. The turnover rate of gut microbiome community was higher during seawater-freshwater transition (1-5 days) than that in later freshwater conditions. Salinity was the major factor leading to the alpha diversity and community dissimilarity of gut microbiome during seawater-freshwater transition, while the host selection showed dominant effects during freshwater stage. The transitivity, connectivity, and average clustering coefficient of gut microbial co-occurrence networks showed decreased trends, while modularity increased during seawater-freshwater migration. For metabolic pathways, "Amino Acid Metabolism" and "Lipid Metabolism" were higher during seawater-freshwater transition than in freshwater. This study advances our mechanistic understanding of the assembly and succession of gut microbiota, which provides new insights into the gut ecology of other aquatic animals.
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http://dx.doi.org/10.3389/fmicb.2022.858508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005979PMC
March 2022

Knockdown or inhibition of arginine kinases enhances susceptibility of Tribolium castaneum to deltamethrin.

Pestic Biochem Physiol 2022 May 17;183:105080. Epub 2022 Mar 17.

College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou 225009, China. Electronic address:

Metabolism of insecticides is an energy-consuming process. As an important component of the intracellular energy buffering system, arginine kinase (AK) plays an important role in insect cellular energy homeostasis and environmental stress response, but the involvement of AKs in the response to chemical stressors (insecticides) remains largely unknown. In this study, using Tribolium castaneum as a model organism, we found that deltamethrin treatment significantly upregulated the expression of TcAK1 and TcAK2 and decreased the whole body ATP content. The knockdown of TcAK1 or TcAK2 significantly enhances the deltamethrin-induced ATP depletion and increase the susceptibility of T. castaneum to deltamethrin. In addition, pretreatment with two AK inhibitors, rutin and quercetin, significantly decreased the lifespan of beetles treated with deltamethrin. These results suggest that AKs might be involved in detoxification of insecticides by regulating cellular energy balance.
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http://dx.doi.org/10.1016/j.pestbp.2022.105080DOI Listing
May 2022

A comprehensive evaluation of organic micropollutants (OMPs) pollution and prioritization in equatorial lakes from mainland Tanzania, East Africa.

Water Res 2022 Jun 5;217:118400. Epub 2022 Apr 5.

State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China. Electronic address:

A lack of understanding the fate of highly toxic organic micropollutants (OMPs) in the equatorial lakes of Tanzania hinders public awareness for protecting these unique aquatic ecosystems, which are precious water resources and stunning wildlife habitats. To address this knowledge gap, the occurrence of 70 anthropogenically-sourced OMPs, including phthalates (PAEs), polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs), was investigated in the water and sediment of 18 lakes in Tanzania. Similar residue concentrations were found in both compartments, showing higher pollution of PAEs ranging from 835.0 to 13,153.1 ng/L in water and 244.6-8691.8 ng/g dw in sediment, followed by PAHs, while OCPs and PCBs were comparatively lower. According to the multi-criteria scoring method for prioritization, the final OMP priority list for the lake environment in Tanzania comprised 25 chemicals, specifically 5 PAEs (DEHP, DIBP, DBP, DCHP and DMPP), 6 PCBs (PCB153, PCB105, PCB28, PCB156, PCB157 and PCB167), 6 PAHs (BaP, BaA, BbF, Pyr, DahA and InP) and 8 OCPs (cis-chlordane, trans-chlordane, p,p'-DDD, p,p'-DDE, p,p'-DDT, endrin, methoxychlor and heptachlor epoxide), suggesting the key substances for conventional monitoring and pollution control in these equatorial lakes, with an emphasis on PAEs, especially DEHP, due to the top priority and endocrine disruptor properties.
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http://dx.doi.org/10.1016/j.watres.2022.118400DOI Listing
June 2022

Identification of a novel circ_0001946/miR-1290/SOX6 ceRNA network in esophageal squamous cell cancer.

Thorac Cancer 2022 05 12;13(9):1299-1310. Epub 2022 Apr 12.

Department of Thoracic Surgery, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, China.

Background: Circular RNAs (circRNAs) can function as competing endogenous RNAs (ceRNAs) to impact the development of esophageal squamous cell cancer (ESCC). Human circ_0001946 has been identified as a potential anticancer factor in ESCC, yet our understanding of its molecular basis remains limited.

Methods: Circ_0001946, microRNA (miR)-1290 and SRY-box transcription factor 6 (SOX6) were quantified by quantitative reasl-time PCR (qRT-PCR) or immunoblotting. Cell proliferation was assessed by CCK-8 and EDU assays. Cell apoptosis and invasion were evaluated by flow cytometry and transwell assays, respectively. Cell migration was detected by transwell and wound-healing assays. The direct relationship between miR-1290 and circ_0001946 or SOX6 was determined by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Xenograft model assays were used to assess the role of circ_0001946 in tumor growth.

Results: Circ_0001946 expression was attenuated in human ESCC, and circ_0001946 increase impeded cell proliferation, invasion, migration and enhanced apoptosis in vitro. Moreover, circ_0001946 increase diminished xenograft growth in vivo. Mechanistically, circ_0001946 bound to miR-1290, and re-expression of miR-1290 reversed circ_0001946-dependent cell properties. SOX6 was a miR-1290 target and it was responsible for the regulation of miR-1290 in cell properties. Furthermore, circ_0001946 functioned as a ceRNA to regulate SOX6 expression via miR-1290.

Conclusion: Our findings uncover an undescribed molecular mechanism, the circ_0001946/miR-1290/SOX6 ceRNA crosstalk, for the anti-ESCC activity of circ_0001946.
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http://dx.doi.org/10.1111/1759-7714.14381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058308PMC
May 2022

Identification and Validation of ATP-Binding Cassette Transporters Involved in the Detoxification of Abamectin in Rice Stem Borer, .

J Agric Food Chem 2022 Apr 12;70(15):4611-4619. Epub 2022 Apr 12.

College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.

has developed high levels of resistance to abamectin in many areas of China, while the underline resistance mechanisms are largely unclear. ATP-binding cassette (ABC) transporters function in transporting a large diversity of substrates including insecticides and play important roles in the detoxification metabolism of insects. In this study, synergism bioassay revealed that the ABC transporters were involved in the detoxification of to abamectin. Six ABC transporter genes were upregulated in after abamectin exposure, among which five genes , , , , and were induced in the detoxification-related tissues. In addition, the five ABC transporters were recombinantly expressed in Sf9 cells, and the cytotoxicity assay showed that the viabilities of cells expressing CsABCC8 or CsABCH1 were significantly increased when compared with the viabilities of cells expressing EGFP after abamectin, chlorantraniliprole, cyantraniliprole, fipronil, and chlorpyrifos treatment, respectively. Overexpression of CsABCE1 significantly increased the viabilities of cells to abamectin, chlorantraniliprole, deltamethrin, and indoxacarb exposure, respectively. These results suggested that CsABCC8, CsABCE1, and CsABCH1 might participate in the detoxification and transport of abamectin and several other classes of insecticides in . Our study provides valuable insights into the transport-related detoxification mechanisms in and other insects.
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http://dx.doi.org/10.1021/acs.jafc.2c00414DOI Listing
April 2022
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