Publications by authors named "Jianjun Luo"

115 Publications

Traditional Chinese Medicine Enema Therapy in a Patient With a Confirmed Negative SARS-CoV-2 Test in the Respiratory Tract but Positive in the Intestinal Tract: A Case Report.

Front Public Health 2021 9;9:687283. Epub 2021 Jul 9.

Department of Clinical Laboratory, West Lake Hospital Affiliated to Hangzhou Medical College, Hangzhou, China.

We report the case of a 43-year-old man who was infected with SARS-CoV-2 in February 2020 and actively cooperated with treatment in the hospital. During the course of treatment, we found that the respiratory SARS-CoV-2 nucleic acid became negative, but remained positive in the intestinal tract. As a result, we adjusted the treatment plan to include traditional Chinese medicine enema treatment. The patient had negative intestinal SARS-CoV-2 nucleic acid test within 4 days, and the subsequent repeated review of intestinal SARS-CoV-2 nucleic acid was negative, and the virus was undetectable. It is suggested that traditional Chinese medicine enema treatment may be helpful to remove the SARS-CoV-2 in the intestines of patients with COVID-19 infection, and may support the treatment of patients with respiratory SARS-CoV-2 nucleic acid negative and positive in the intestinal tract.
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http://dx.doi.org/10.3389/fpubh.2021.687283DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299274PMC
July 2021

Acute hepatic ischemia after transjugular intrahepatic portosystemic shunt creation despite preserved arterial perfusion.

J Vasc Interv Radiol 2021 Jul 20. Epub 2021 Jul 20.

Shanghai Institute of Medical Imaging, Shanghai, China; Department of Interventional Radiology, Zhongshan Hospital, Fudan University, Shanghai, China; National Clinical Research Center for Interventional Medicine,Zhongshan Hospital,Fudan University, Shanghai, China; Center for Tumor Diagnosis and Therapy,Jinshan Hospital,Fudan University,Shanghai,China. Electronic address:

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http://dx.doi.org/10.1016/j.jvir.2021.07.007DOI Listing
July 2021

LncRNA-encoded microproteins: A new form of cargo in cell culture-derived and circulating extracellular vesicles.

J Extracell Vesicles 2021 Jul 12;10(9):e12123. Epub 2021 Jul 12.

Laboratory of Protein and Peptide Pharmaceuticals and Laboratory of Proteomics Institute of Biophysics Chinese Academy of Sciences Beijing China.

Advancements in omics-based technologies over the past few years have led to the discovery of numerous biologically relevant peptides encoded by small open reading frames (smORFs) embedded in long noncoding RNA (lncRNA) transcripts (referred to as microproteins here) in a variety of species. However, the mechanisms and modes of action that underlie the roles of microproteins have yet to be fully characterized. Herein, we provide the first experimental evidence of abundant microproteins in extracellular vesicles (EVs) derived from glioma cancer cells, indicating that the EV-mediated transfer of microproteins may represent a novel mechanism for intercellular communication. Intriguingly, when examining human plasma, 48, 11 and 3 microproteins were identified from purified EVs, whole plasma and EV-free plasma, respectively, suggesting that circulating microproteins are primarily enriched in EVs. Most importantly, the preliminary data showed that the expression profile of EV microproteins in glioma patient diverged from the health donors, suggesting that the circulating microproteins in EVs might have potential diagnostic application in identifying patients with glioma.
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http://dx.doi.org/10.1002/jev2.12123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275822PMC
July 2021

Deeply Mining a Universe of Peptides Encoded by Long Noncoding RNAs.

Mol Cell Proteomics 2021 Jun 12;20:100109. Epub 2021 Jun 12.

Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China. Electronic address:

Many small ORFs embedded in long noncoding RNA (lncRNA) transcripts have been shown to encode biologically functional polypeptides (small ORF-encoded polypeptides [SEPs]) in different organisms. Despite some novel SEPs have been found, the identification is still hampered by their poor predictability, diminutive size, and low relative abundance. Here, we take advantage of NONCODE, a repository containing the most complete collection and annotation of lncRNA transcripts from different species, to build a novel database that attempts to maximize a collection of SEPs from human and mouse lncRNA transcripts. In order to further improve SEP discovery, we implemented two effective and complementary polypeptide enrichment strategies using 30-kDa molecular weight cutoff filter and C8 solid-phase extraction column. These combined strategies enabled us to discover 353 SEPs from eight human cell lines and 409 SEPs from three mouse cell lines and eight mouse tissues. Importantly, 19 of them were then verified through in vitro expression, immunoblotting, parallel reaction monitoring, and synthetic peptides. Subsequent bioinformatics analysis revealed that some of the physical and chemical properties of these novel SEPs, including amino acid composition and codon usage, are different from those commonly found in canonical proteins. Intriguingly, nearly 65% of the identified SEPs were found to be initiated with non-AUG start codons. The 762 novel SEPs probably represent the largest number of SEPs detected by MS reported to date. These novel SEPs might not only provide new clues for the annotation of noncoding elements in the genome but also serve as a valuable resource for functional study.
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http://dx.doi.org/10.1016/j.mcpro.2021.100109DOI Listing
June 2021

Instantaneous Self-Powered Sensing System Based on Planar-Structured Rotary Triboelectric Nanogenerator.

Sensors (Basel) 2021 May 28;21(11). Epub 2021 May 28.

CAS Center for Excellence in Nanoscience, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, China.

Self-powering electronics by harvesting mechanical energy has been widely studied, but most self-powering processes require a long time in the energy harvesting procedure, resulting in low efficiency or even system failure in some specific applications such as instantaneous sensor signal acquisition and transmission. In order to achieve efficient self-powered sensing, we design and construct an instantaneous self-powered sensing system, which puts heavy requirements on generator's power and power management circuit. Theoretical analysis and experimental results over two types of generators prove that the planar-structured rotary triboelectric nanogenerator possesses many advantages over electromagnetic generator for the circumstances of instantaneous self-powering. In addition, an instantaneous driving mode power management circuit is also introduced showing advanced performance for the instantaneous self-powering sensing system. As a proof-of-concept, an integrated instantaneous self-powered sensing system is demonstrated based on Radio-Frequency transmission. This work demonstrates the potential of instantaneous self-powered sensing systems to be used in a wide range of applications such as smart home, environment monitoring, and security surveillance.
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http://dx.doi.org/10.3390/s21113741DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197852PMC
May 2021

Discovery of Plasma Membrane-Associated RNAs through APEX-seq.

Cell Biochem Biophys 2021 May 24. Epub 2021 May 24.

Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China.

In addition to nucleic acids, a variety of other biomolecules have also been found on the plasma membrane. Although researchers have realized that RNA has the ability to bind to membrane vesicles in vitro, little is known about whether and how RNA connects to the plasma membrane of the cell. The combination of high-throughput sequencing and in situ labeling methods provides an innovative approach for large-scale identification of subcellular RNAs. Here, we applied the recently published method APEX-seq and identified 75 RNAs related to the plasma membrane, in which lncRNA PMAR72 (plasma membrane-associated RNA AL121772.1) has a considerable affinity with sphingomyelin (SM) and localizes within distinct membrane foci. Our findings will provide some new evidence to elaborate the relationship between RNA and the plasma membrane of mammalian cells.
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http://dx.doi.org/10.1007/s12013-021-00991-0DOI Listing
May 2021

Long noncoding RNA AVAN promotes antiviral innate immunity by interacting with TRIM25 and enhancing the transcription of FOXO3a.

Cell Death Differ 2021 May 14. Epub 2021 May 14.

Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, Beijing, China.

Accumulating evidence has shown that long noncoding RNAs (lncRNAs) are involved in several biological processes, including immune responses. However, the role of lncRNAs in antiviral innate immune responses remains largely elusive. Here, we identify an uncharacterized human lncRNA AVAN from influenza A virus (IAV) infected patients, that is significantly upregulated following RNA virus infection. During IAV infection, AVAN play an indispensable role in antiviral immune responses. In vivo, we enforced the expression of AVAN in transgenic mice or adeno-associated virus encoding AVAN delivery system and found that AVAN significantly alleviated IAV virulence and virus replication. Mechanistically, nuclear AVAN positively regulates the transcription of forkhead box O3A (FOXO3a) by associating with its promoter and inducing chromatin remodeling to promote neutrophil chemotaxis. Meanwhile, cytoplasmic AVAN binds directly to the E3 ligase TRIM25 and enhances TRIM25-mediated K63-linked ubiquitination of RIG-I, thereby promoting TRIM25- and RIG-I-mediated antiviral innate immune responses, including the induction of type I interferon and ISGs. Moreover, AVAN binds to the B Box/CCD domain of TRIM25 and 1-200nt of AVAN were the functional moieties. Collectively, our findings highlight the potential clinical implications of human lncRNA AVAN as a key positive regulator of the antiviral innate immune response and a promising target for developing broad antiviral therapeutics.
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http://dx.doi.org/10.1038/s41418-021-00791-2DOI Listing
May 2021

From Green Revolution to Green Balance: The Nitrogen and Gibberellin Mediated Rice Tiller Growth.

Plant Signal Behav 2021 Jul 25;16(7):1917838. Epub 2021 Apr 25.

State Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Hunan 410004, China.

Rice tillering is an important characteristic that responds to both GA (gibberellin) and nitrogen-based fertilizers. How plants balance these two responses? A newly identified NGR5 (NITROGEN-MEDIATED TILLER GROWTH RESPONSE 5) protein reveals its important role in controlling the balance between GA-regulated dwarfism and nitrogen-regulated tillering. NGR5 directly interacts with PRC2 (Polycomb Repressive Complex 2) to form a repressive complex at the shoot branching inhibitory genes in nitrogen-dependent way, thereby repressing branching inhibitors and promoting tillering in response to nitrogen fertilizers. The GA receptor GID1 (GIBBERELLIN INSENSITIVE DWARF1) targets NGR5 for proteolysis by the 26S proteasome. The rice DELLA proteins of GA signaling way competitively inhibit GID1-NGR5 interaction, thereby protecting NGR5 from degradation and enhancing nitrogen-induced tiller number.
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http://dx.doi.org/10.1080/15592324.2021.1917838DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205009PMC
July 2021

Circular RNA_0000629 Suppresses Bladder Cancer Progression Mediating MicroRNA-1290/CDC73.

Cancer Manag Res 2021 22;13:2701-2715. Epub 2021 Mar 22.

Department of Urology, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410005, Hunan, People's Republic of China.

Background: Recent studies showed circular RNAs (circRNAs) played regulatory roles in bladder cancer (BC). However, the relevance of circ_0000629, a newly identified circRNA, has not been determined yet. We aimed to characterize the function of circ_0000629 in BC and the relevant mechanism.

Methods: First, we downloaded circRNA-related microarrays GSE147985 and GSE92675 from the GEO database, followed by a validation in our clinically obtained samples. We then overexpressed circ_0000629 in T24 and SW780 cells and evaluated the effects of circ_0000629 on BC cell proliferatory, apoptotic, and metastatic abilities. We further detected the subcellular localization of circ_0000629 in T24 and SW780 cells by the fractionation and export assay and FISH experiments. Integrated microarray analyses and bioinformatics website prediction were utilized to screen out the downstream microRNA (miRNA)/mRNA. The effects of miR-1290 and CDC73 on BC cell growth and metastasis was verified by functional rescue experiments. In addition, mice xenografts were built to measure the effect of circ_0000629 on tumor growth in vivo.

Results: Circ_0000629 and CDC73 were reduced, and miR-1290 was significantly overexpressed in BC tissues and cells. Moreover, circ_0000629 significantly inhibited the development and metastasis of BC cells, but further overexpression of miR-1290 or knockdown of CDC73 attenuated the inhibitory effect of circ_0000629 on BC cells. Circ_0000629 localized in the cytoplasm and regulated CDC73 expression by sponging miR-1290. Further, overexpressed circ_0000629 reduced the BC tumor growth in vivo.

Conclusion: Circ_0000629 promotes the expression of CDC73 by competitively binding to miR-1290, thereby inhibiting the growth and metastasis of BC cells.
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http://dx.doi.org/10.2147/CMAR.S292863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997432PMC
March 2021

The Triboelectric Nanogenerator as an Innovative Technology toward Intelligent Sports.

Adv Mater 2021 Apr 24;33(17):e2004178. Epub 2021 Mar 24.

CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Micro-nano Energy and Sensor, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, P. R. China.

In the new era of the Internet-of-Things, athletic big data collection and analysis based on widely distributed sensing networks are particularly important in the development of intelligent sports. Conventional sensors usually require an external power supply, with limitations such as limited lifetime and high maintenance cost. As a newly developed mechanical energy harvesting and self-powered sensing technology, the triboelectric nanogenerator (TENG) shows great potential to overcome these limitations. Most importantly, TENGs can be fabricated using wood, paper, fibers, and polymers, which are the most frequently used materials for sports. Recent progress on the development of TENGs for the field of intelligent sports is summarized. First, the working mechanism of TENG and its association with athletic big data are introduced. Subsequently, the development of TENG-based sports sensing systems, including smart sports facilities and wearable equipment is highlighted. At last, the remaining challenges and open opportunities are also discussed.
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http://dx.doi.org/10.1002/adma.202004178DOI Listing
April 2021

Efficacy of puncturing different portal vein branch during transjugular intrahepatic portosystemic shunt with 8 mm covered stent: a propensity-score analysis.

Eur J Gastroenterol Hepatol 2021 Aug;33(8):1110-1116

Department of Interventional Radiology, Zhongshan Hospital, Fudan University.

Background: Hepatic encephalopathy after transjugular intrahepatic portosystemic shunt (TIPS) remains an unsolved problem; therefore, this study aimed to compare the efficacy of shunting different portal vein branch during TIPS with 8 mm covered stent for preventing gastroesophageal variceal rebleeding in cirrhotic patients.

Methods: Between November 2015 and December 2018, the medical records of consecutive cirrhotic patients who received TIPS with an 8 mm covered stent for preventing gastroesophageal variceal rebleeding were analyzed retrospectively. Shunting the left and right branches of the portal vein was performed in 58 (group A) and 104 patients (group B), respectively. Afterwards, 54 patients in each group were produced by the PSM method. The incidence of overt hepatic encephalopathy (OHE), shunt patency, variceal rebleeding and survival were compared between two groups by using Kaplan-Meier analysis and log-rank test.

Results: The median follow-up time was 37 months (range 0.3-50 months). The 6-,12- and 24-months cumulative OHE free rates in groups A and B were 92.5, 86.2, 83.6% and 74.1, 70.2, 67.6%, respectively (P = 0.042; hazard ratio = 0.469; 95% CI, 0.223-0.988). Two (3.7%) and eight (14.8%) patients experienced severe OHE in groups A and B, respectively (P = 0.042). There were no significant differences between the two groups in terms of shunt patency, variceal rebleeding and survival.

Conclusion: Shunting the left portal vein branch during TIPS with an 8 mm covered stent for preventing gastroesophageal variceal rebleeding in cirrhotic patients might decrease the occurrence of OHE significantly. The prospective trial is needed to further provide evidence of this results in future.
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http://dx.doi.org/10.1097/MEG.0000000000002133DOI Listing
August 2021

Nomogram for Predicting the Prognosis of Patients with Hepatocellular Carcinoma Presenting with Pulmonary Metastasis.

Cancer Manag Res 2021 1;13:2083-2094. Epub 2021 Mar 1.

Department of Interventional Radiology, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China.

Background: Only a few studies have investigated the clinical features and outcomes of patients with pulmonary metastasis from hepatocellular carcinoma (HCC) at the initial diagnosis. This study aimed to evaluate the prevalence, risk factors and prognostic factors related to pulmonary metastasis and then construct a nomogram to predict the outcomes of patients with HCC presenting with pulmonary metastasis.

Methods: The Surveillance, Epidemiology, and End Results (SEER) database was used to select patients. A total of 25,236 eligible patients diagnosed with HCC from 2010 to 2015 were selected. Then, 897 patients with HCC presenting with pulmonary metastasis at the initial diagnosis were included in the primary set (n=598) and validation set (n=299). Logistic and Cox regression analyses were used to determine the risk factors and prognostic factors for pulmonary metastasis. A nomogram predicting the prognosis of patients with HCC presenting with pulmonary metastasis was constructed based on independent prognostic factors identified in Cox regression analyses. Both internal and external validations of the nomogram were performed using discrimination and calibration plots.

Results: The prevalence of pulmonary metastasis was 3.6% (897/25,236) in the entire cohort diagnosed with HCC as the initial diagnosis. Age, race, Edmonson-Steiner classification grade I/III, higher T stage, N stage, alpha fetoprotein(AFP) levels, brain metastasis, bone metastasis and intrahepatic metastasis were positively correlated with the development of HCC with pulmonary metastasis at the initial diagnosis. Prognostic factors incorporated in the nomogram were sex, T stage, bone metastasis, AFP levels, treatment, radiation and chemotherapy. The concordance index (C-index) of the nomogram in the primary set was 0.661 (95% CI: 0.633-0.688), indicating considerable predictive accuracy. The calibration curves showed consistency between the nomogram and the actual observations. When the nomogram was applied to the validation set, the results also remained reconcilable, and the C-index of the nomogram was 0.657 (95% CI: 0.626-0.698).

Conclusion: A list of risk factors associated with pulmonary metastasis occurrence in patients with HCC was selected, and the nomogram accurately predicted the prognosis of patients with HCC presenting with pulmonary metastasis at the initial diagnosis.
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http://dx.doi.org/10.2147/CMAR.S296020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935331PMC
March 2021

Data-driven game-based control of microsatellites for attitude takeover of target spacecraft with disturbance.

ISA Trans 2021 Feb 27. Epub 2021 Feb 27.

Research and Development Institute of Northwestern Polytechnical University in Shenzhen, Shenzhen, 518057, China; Science and Technology on Aerospace Flight Dynamics Laboratory, Northwestern Polytechnical University, Xi'an, 710072, China.

This paper investigates the problem of using multiple microsatellites to control the attitude of a target spacecraft losing control ability. Considering external disturbance and unknown system dynamics, a data-driven robust control method based on game theory is proposed. Firstly, the attitude takeover control of the target using multiple microsatellites is modeled as a robust differential game among disturbance and multiple microsatellites, in which microsatellites can obtain the worst-case control policies. Subsequently, policy iteration algorithm is put forward to acquire the robust Nash equilibrium control policies of microsatellites with known dynamics, which is a basis of data-driven algorithm. Then, by employing off-policy integral reinforcement learning, a data-driven online controller without information about system dynamics is developed to get the feedback gain matrices of microsatellites by learning robust Nash equilibrium solution from online input-state data. To validate the effectiveness of the proposed control method, numerical simulations are provided.
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http://dx.doi.org/10.1016/j.isatra.2021.02.037DOI Listing
February 2021

Dysregulation of USP18/FTO/PYCR1 signaling network promotes bladder cancer development and progression.

Aging (Albany NY) 2021 01 10;13(3):3909-3925. Epub 2021 Jan 10.

Department of Urology, The Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China.

N6-methyladenosine refers to a methylation of adenosine base at the 6 nitrogen position, which is the dominant methylation modification in both message and non-coding RNAs. Dysregulation of RNA m6A methylation causes tumorigenesis in humans. The key N6-methyladenosine demethylase fat-mass and obesity-associated protein (FTO) is negatively correlated with the overall survival of bladder cancer patients, but the underlying mechanism remains poorly understood. In this study, we demonstrated that the post-translational deubiquitination by USP18 up-regulates the protein but not mRNA of FTO in bladder cancer tissues and cells. As a result, FTO decreased N6-methyladenosine methylation level in through its demethylase enzymatic activity and stabilized transcript to promote bladder cancer initiation and progression. Our work shows the importance of N6-methyladenosine RNA modification in bladder cancer development, and highlights UPS18/FTO/PYCR1 signaling network as potential therapeutic targets of bladder cancer.
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http://dx.doi.org/10.18632/aging.202359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906198PMC
January 2021

Endovascular implantation of iodine-125 seed strand combined and stent placement for locally advanced pancreatic ductal adenocarcinoma with vascular invasion: a prospective single-arm pilot study.

J Contemp Brachytherapy 2020 Oct 30;12(5):427-434. Epub 2020 Oct 30.

Department of Interventional Radiology, Affiliated Zhongshan Hospital of Fudan University, Xuhui District, Shanghai, China.

Purpose: To investigate the safety and feasibility of endovascular brachytherapy using iodine-125 (I) seed strand for locally advanced pancreatic ductal adenocarcinoma (PDAC) with vascular invasion.

Material And Methods: From January 2010 to January 2015, 12 patients diagnosed with locally advanced, inoperable PDAC with splenic or superior mesenteric vein invasion were enrolled in the present study and received endovascular brachytherapy combined with regional intra-arterial infusion chemotherapy. Standardized software was used for dose calculation. Procedure-related and radiation complications were documented and assessed. Overall survival was calculated with the Kaplan-Meier approach.

Results: The technical success rate of I seed strand implantation and stent placement was 100%. During follow-up with a mean duration of 17.00 ±6.07 months (range, 6~24 months), the mean and median survival times were 12.0 ±2.4 months (95% CI: 7.4~16.6 months) and 10.7 ±1.4 months (95% CI: 8.0~13.5 months), respectively. One month after the treatment, the disease control and objective rates were 83.8% and 58.3%, respectively. The 6-, 12-, and 15-month cumulative survival rates were 66.7%, 47.6%, and 9.5%, respectively.

Conclusions: Endovascular brachytherapy using I seed strand and stent placement may be a safe and effective treatment option for locally advanced pancreatic duct adenocarcinoma with vascular invasion.
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http://dx.doi.org/10.5114/jcb.2020.100375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701926PMC
October 2020

Impact of Intrahepatic Venovenous Shunt on Hepatic Venous Pressure Gradient Measurement.

J Vasc Interv Radiol 2020 Dec;31(12):2081-2088

Department of Interventional Radiology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China; Shanghai Institution of Medical Imaging, Shanghai, China.

Purpose: To quantitatively analyze the impact of intrahepatic venovenous shunt (IHVS) on hepatic venous pressure gradient (HVPG) measurement.

Materials And Methods: From 2015 to 2019, 222 HVPG measurements performed during transjugular intrahepatic portosystemic shunt creation were eligible for this study. Digital subtraction angiography (DSA) software color-coded each pixel of a two-dimensional DSA series by time-intensity curve to classify IHVS. Different degrees of IHVS were found in 36.5% of patients (81/222). Mild IHVS was found in 10.8% of patients (24/222), moderate IHVS was found in 10.8% of patients (24/222), and severe IVHS was found in 14.9% of patients (33/222).

Results: Mean wedged hepatic vein pressure (WHVP) and HVPG were significantly lower in patients with IHVS compared with patients without IHVS (WHVP: 17.78 mm Hg ± 7.00 vs 24.89 mm Hg ± 8.69, P = .001; HVPG: 11.93 mm Hg ± 5.76 vs 18.6 mm Hg ± 6.85, P < .001). Mild IHVS had little effect on WHVP and HVPG. Mean WHVP and HVPG were 11 mm Hg lower in patients with moderate IHVS (WHVP: 20.38 mm Hg ± 8.38 vs 31.5 mm Hg ± 9.39, P = .026; HVPG: 13.88 mm Hg ± 6.33 vs 25.00 mm Hg ± 9.81, P < .001) and 15 mm Hg lower in patients with severe IHVS (WHVP: 13.45 mm Hg ± 5.28 vs 28.64 mm Hg ± 6.38, P = .017; HVPG: 8.27 mm Hg ± 3.85 vs 23.45 mm Hg ± 6.95, P < .001) than mean portal vein pressure and portal vein gradient.

Conclusions: For patients with moderate or severe IHVS, HVPG might greatly underestimate the actual value of portal vein pressure, and the portal vein should be catheterized to measure portal pressure.
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http://dx.doi.org/10.1016/j.jvir.2020.08.027DOI Listing
December 2020

Genetic variations associated with long noncoding RNAs.

Essays Biochem 2020 12;64(6):867-873

Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

Genetic variations, including single nucleotide polymorphisms (SNPs) and structural variations, are widely distributed in the genome, including the long noncoding RNA (lncRNA) regions. The changes at locus might produce numerous effects in a variety of aspects. Multiple bioinformatics resources and tools were also developed for systematically dealing with genetic variations associated with lncRNAs. Moreover, correlation of the genetic variations in lncRNAs with immune disease, cancers, and other disease as well as development process were all included for discussion. In this essay, we summarized how and in what aspects these changes would affect lncRNA functions.
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http://dx.doi.org/10.1042/EBC20200033DOI Listing
December 2020

Which Household Emergency Plans are More Helpful in Tornadoes? Through the Lens of Gerontology.

Disaster Med Public Health Prep 2020 Sep 9:1-8. Epub 2020 Sep 9.

Air Worldwide, Boston, Massachusetts.

Objective: This study investigated how the effectiveness of household emergency plans during tornadoes was associated with family discussions, and the attributes of the plan for different age groups.

Methods: A telephone survey was conducted in 2014, one year after two 2013 Enhanced Fujita 4/5 tornadoes. The working sample included 223 respondents who reported having a household emergency plan before the tornadoes. The latent class analysis was used to identify the patterns of the plans and develop a typology based on their content. Logistic regression was used to examine predictors for plan effectiveness.

Results: Two classes of plans were identified: quality plans that were rich in content and limited plans that had lower levels of content richness. Older adults were less likely to have quality plans and less likely to have family discussions. Quality of the plan and discussions with family members increased plan effectiveness among older adults, but not younger adults.

Conclusions: Better emergency planning could be more important for older than for younger adults. The findings were discussed from a gerontological perspective that focuses on older adults' unique needs, vulnerabilities, and resilience factors.
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http://dx.doi.org/10.1017/dmp.2020.194DOI Listing
September 2020

Effects of Surface Functional Groups on Electron Transfer at Liquid-Solid Interfacial Contact Electrification.

ACS Nano 2020 Aug 3;14(8):10733-10741. Epub 2020 Aug 3.

Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, People's Republic of China.

Contact electrification (CE) at interfaces is sensitive to the functional groups on the solid surface, but its mechanism is poorly understood, especially for the liquid-solid cases. A core controversy is the identity of the charge carriers (electrons or/and ions) in the CE between liquids and solids. Here, the CE between SiO surfaces with different functional groups and different liquids, including DI water and organic solutions, is systematically studied, and the contribution of electron transfer is distinguished from that of ion transfer according to the charge decay behavior at surfaces at specific temperature, because electron release follows the thermionic emission theory. It is revealed that electron transfer plays an important role in the CE between liquids and functional group modified SiO. Moreover, the electron transfer between the DI water and the SiO is found highly related to the electron affinity of the functional groups on the SiO surfaces, while the electron transfer between organic solutions and the SiO is independent of the functional groups, due to the limited ability of organic solutions to donate or gain electrons. An energy band model for the electron transfer between liquids and solids is further proposed, in which the effects of functional groups are considered. The discoveries in this work support the "two-step" model about the formation of an electric double-layer (Wang model), in which the electron transfer occurs first when the liquids contact the solids for the very first time.
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http://dx.doi.org/10.1021/acsnano.0c06075DOI Listing
August 2020

PTFE-covered TIPS is an effective treatment for secondary preventing variceal rebleeding in cirrhotic patients with high risks.

Eur J Gastroenterol Hepatol 2020 09;32(9):1235-1243

Department of Gastroenterology.

Aim: To compare the effectiveness and safety of transjugular intrahepatic portosystemic shunt (TIPS) with endoscopic therapy plus non-selective β-blockers (NSBBs) for secondary prevention of gasroesophageal variceal bleeding (GEVB) in cirrhotic patients with high-risk factors of treatment failure.

Methods And Material: A total of 122 cirrhotic patients with history of gasroesophageal variceal bleeding and high factors including hepatic vein pressure gradient (HVPG) ≥ 20 mmHg, portal vein thrombosis (PVT), gastrorenal shunt (GRS), or extraluminal para-gastric veins (ep-GVs) detected by endoscopic ultrasound, were analyzed retrospectively. Seventy-seven patients underwent TIPS with PTFE-covered stent (group A) and 102 patients received endoscopic therapy combined with nonselective β-blockers (NSBBs) (group B). According to above high-risk factors, both groups were stratified into four paired subgroups (A1-A4 and B1-B4). Two-year rebleeding rate, overt hepatic encephalopathy, overall survival, and procedure-related adverse events were compared between the two groups and paired subgroups.

Results: The 2-year cumulative probability of free of variceal rebleeding was higher in group A than group B (93 vs. 62%, P < 0.001). Similarly, the 2-year cumulative probability of free of variceal rebleeding was also higher in the subgroups A1-A4 than the subgroups B1-B4 (91 vs. 67%, P = 0.022, 90 vs. 67%, P = 0.021, 94 vs. 59%, P = 0.029, and 90 vs. 58%, P = 0.016, respectively). There was no significant difference between the two groups and corresponding subgroups in overt hepatic encephalopathy and survival.

Conclusion: Compared to secondary prophylaxis with endoscopic therapy plus NSBBs, polytetrafluoroethylene-covered TIPS could significantly reduce the variceal rebleeding rate in cirrhotic patients with HVPG ≥ 20 mmHg, PVT, GRS, or ep-GVs, without increasing the incidence of hepatic encephalopathy.
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http://dx.doi.org/10.1097/MEG.0000000000001686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7423526PMC
September 2020

Effectiveness of Household Emergency Plans in Violent Tornadoes.

Disaster Med Public Health Prep 2020 Jul 13:1-2. Epub 2020 Jul 13.

Catastrophe Modeling and Risk Assessment, AIR Worldwide, Boston, MA.

Objective: This study examined factors that were associated with the effectiveness of pre-existing household emergency plans during the 2011 EF5 Joplin and EF4 Tuscaloosa tornadoes. We focused on whether discussing with family members helped increase the plan's effectiveness.

Methods: A telephone survey based on random sampling was conducted in 2012 with 1006 respondents in both cities. Each city experienced huge losses, injuries, and casualties. The working sample included 494 respondents who had a household emergency plan in place before these tornadoes.

Results: Multinomial logistic regression showed that discussing with family members increased the helpfulness of the plan in Joplin, where people had not experienced tornadoes frequently and were less prepared for tornadoes relative to residents in Tuscaloosa.

Conclusions: This study provides empirical evidence on the importance of encouraging family involvement when making household emergency plans, especially in places that are less prepared for disasters than those that are better prepared.
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http://dx.doi.org/10.1017/dmp.2020.129DOI Listing
July 2020

A Self-Powered Angle Sensor at Nanoradian-Resolution for Robotic Arms and Personalized Medicare.

Adv Mater 2020 Aug 30;32(32):e2001466. Epub 2020 Jun 30.

CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Micro-nano Energy and Sensor, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, P. R. China.

As the dominant component for precise motion measurement, angle sensors play a vital role in robotics, machine control, and personalized rehabilitation. Various forms of angle sensors have been developed and optimized over the past decades, but none of them would function without an electric power. Here, a highly sensitive triboelectric self-powered angle sensor (SPAS) exhibiting the highest resolution (2.03 nano-radian) after a comprehensive optimization is reported. In addition, the SPAS holds merits of light weight and thin thickness, which enables its extensive integrated applications with minimized energy consumption: a palletizing robotic arm equipped with the SPAS can precisely reproduce traditional Chinese calligraphy via angular data it collects. In addition, the SPAS can be assembled in a medicare brace to record the flexion/extension of joints, which may benefit personalized orthopedic recuperation. The SPAS paves a new approach for applications in the emerging fields of robotics, sensing, personalized medicare, and artificial intelligence.
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http://dx.doi.org/10.1002/adma.202001466DOI Listing
August 2020

LRIK interacts with the Ku70-Ku80 heterodimer enhancing the efficiency of NHEJ repair.

Cell Death Differ 2020 12 25;27(12):3337-3353. Epub 2020 Jun 25.

Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, PR China.

Despite recent advances in our understanding of the function of long noncoding RNAs (lncRNAs), their roles and functions in DNA repair pathways remain poorly understood. By screening a panel of uncharacterized lncRNAs to identify those whose transcription is induced by double-strand breaks (DSBs), we identified a novel lncRNA referred to as LRIK that interacts with Ku, which enhances the ability of the Ku heterodimer to detect the presence of DSBs. Here, we show that depletion of LRIK generates significantly enhanced sensitivity to DSB-inducing agents and reduced DSB repair efficiency. In response to DSBs, LRIK enhances the recruitment of repair factors at DSB sites and facilitates γH2AX signaling. Our results demonstrate that LRIK is necessary for efficient repairing DSBs via nonhomologous end-joining pathway.
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http://dx.doi.org/10.1038/s41418-020-0581-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7852670PMC
December 2020

Shape adaptable and highly resilient 3D braided triboelectric nanogenerators as e-textiles for power and sensing.

Nat Commun 2020 06 8;11(1):2868. Epub 2020 Jun 8.

Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, P. R. China.

Combining traditional textiles with triboelectric nanogenerators (TENGs) gives birth to self-powered electronic textiles (e-textiles). However, there are two bottlenecks in their widespread application, low power output and poor sensing capability. Herein, by means of the three-dimensional five-directional braided (3DB) structure, a TENG-based e-textile with the features of high flexibility, shape adaptability, structural integrity, cyclic washability, and superior mechanical stability, is designed for power and sensing. Due to the spatial frame-column structure formed between the outer braided yarn and inner axial yarn, the 3DB-TENG is also endowed with high compression resilience, enhanced power output, improved pressure sensitivity, and vibrational energy harvesting ability, which can power miniature wearable electronics and respond to tiny weight variations. Furthermore, an intelligent shoe and an identity recognition carpet are demonstrated to verify its performance. This study hopes to provide a new design concept for high-performance textile-based TENGs and expand their application scope in human-machine interfacing.
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http://dx.doi.org/10.1038/s41467-020-16642-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280288PMC
June 2020

Application of CT-based radiomics in predicting portal pressure and patient outcome in portal hypertension.

Eur J Radiol 2020 May 2;126:108927. Epub 2020 Mar 2.

Department of Gastroenterology,Zhongshan Hosptial, Fudan University, China; Department of Endoscopy Center, Zhongshan Hospital, Fudan University, China; Evidence-Based Medicine Center, Fudan University, China. Electronic address:

Purpose: Portal venous pressure (PVP) measurement is of clinical significance, especially in patients with portal hypertension. However, the invasive nature and associated complications limits its application. The aim of the study is to propose a noninvasive predictive model of PVP values based on CT-extracted radiomic features.

Methods: Radiomics PVP (rPVP) models based on liver, spleen and combined features were established on an experimental cohort of 169 subjects. Radiomics features were extracted from each ROI and reduced via the LASSO regression to achieve an optimal predictive formula. A validation cohort of 62 patients treated for gastroesophageal varices (GOV) was used to confirm the utility of rPVP in predicting variceal recurrence. The association between rPVP and response to treatment was observed.

Results: Three separate predictive formula for PVP were derived from radiomics features. rPVP was significantly correlated to patient response to endoscopic treatment for GOV. Among which, the model containing both liver and spleen features has the highest predictability of variceal recurrence, with an optimal cut-off value at 29.102 mmHg (AUC 0.866). A Kaplan Meier analysis further confirmed the difference between patients with varying rPVP values.

Conclusion: PVP values can be accurately predicted by a non-invasive, CT derived radiomics model. rPVP serves as a non-invasive and precise reference for predicting treatment outcome for GOV secondary to portal hypertension.
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http://dx.doi.org/10.1016/j.ejrad.2020.108927DOI Listing
May 2020

Wind-Driven Radial-Engine-Shaped Triboelectric Nanogenerators for Self-Powered Absorption and Degradation of NO.

ACS Nano 2020 Mar 14;14(3):2751-2759. Epub 2020 Feb 14.

CAS Center for Excellence in Nanoscience, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, People's Republic of China.

As one of the major air pollutants, NO is rather challenging to remove. The main treatment method is catalytic reduction with plenty of reducing agents, which lacks any effective control in an open air environment such as urban spaces. It is necessary to seek a self-powered electrochemical process for environmental treatment. The triboelectric nanogenerator (TENG), a developing technology with various advantages, is widely used in energy and environmental monitoring and cleaning. In this work, a radial-engine-shaped TENG system with five stacked TENGs is designed to synchronously absorb NO and degrade its main enrichment forms of nitrate and nitrite in aqueous solution. In addition, the system possesses inherent phase differences and outputs continuous direct current after rectification. Moreover, we demonstrated that, driven by artificial wind at a speed of 6 m/s, the NO generated by a chemical method was effectively degraded by the radial-engine-shaped TENG system.
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http://dx.doi.org/10.1021/acsnano.9b08496DOI Listing
March 2020

LncRNA HRCEG, regulated by HDAC1, inhibits cells proliferation and epithelial-mesenchymal-transition in gastric cancer.

Cancer Genet 2020 02 8;241:25-33. Epub 2020 Jan 8.

Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China; University of Chinese Academy of Sciences, Beijing, 100049, China; Guangdong Geneway Decoding Bio-Tech Co. Ltd, Foshan, 528316, China. Electronic address:

Recently, a number of long noncoding RNAs (lncRNAs) have been reported to play significant roles in human tumorigenesis. However, only few gastric cancer related lncRNAs have been well characterized. Here, we identified one lncRNA HRCEG, whose expression was decreased in the gastric cancer tissues compared with adjacent normal tissues. Overexpression of HRCEG significantly promoted cell apoptosis and inhibited cell proliferation. Importantly, we demonstrated that HRCEG levels inversely correlated with EMT process and HRCEG was regulated by the histone deacetylase 1 (HDAC1) in gastric cancer. These findings suggest that HRCEG might be regulated by HDAC1 to inhibit gastric cancer progress and metastatic capability via EMT pathway.
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http://dx.doi.org/10.1016/j.cancergen.2019.12.007DOI Listing
February 2020

Association of the Polymorphism rs13259960 in SLEAR With Predisposition to Systemic Lupus Erythematosus.

Arthritis Rheumatol 2020 06 26;72(6):985-996. Epub 2020 Apr 26.

Chinese Academy of Sciences and University of Chinese Academy of Sciences, Beijing, China, and Guangdong Geneway Decoding Bio-Tech Co. Ltd, Foshan, China.

Objective: Genome-wide association studies have identified many susceptibility loci for systemic lupus erythematosus (SLE). However, most of these loci are located in noncoding regions of the genome. Long noncoding RNAs (lncRNAs) are pervasively expressed and have been reported to be involved in various diseases. This study aimed to explore the genetic significance of lncRNAs in SLE.

Methods: A genome-wide survey of SLE risk variants in lncRNA gene loci was performed in Han Chinese subjects (4,556 with SLE and 9,451 healthy controls). The functional relevance of an SLE risk variant in one of the lncRNA genes was explored using biochemical and molecular cell biology analyses. In vitro loss-of-function and gain-of-function strategies were used to clarify the functional and phenotypic relevance of this SLE susceptibility lncRNA. Moreover, correlation of this lncRNA with the degree of apoptosis in the peripheral blood of SLE patients was evaluated.

Results: A novel SLE susceptibility locus in a lncRNA gene, designated SLEAR (for SLE-associated RNA), was identified at the single-nucleotide polymorphism rs13259960 (odds ratio 1.35, P = 1.03 × 10 ). The A>G variation at rs13259960, located in an intronic enhancer, was found to impair STAT1 recruitment to the enhancer that loops to the SLEAR promoter, resulting in decreased SLEAR production in peripheral blood mononuclear cells from patients with SLE (3 with the G/G genotype, 22 with A/G, and 103 with A/A at rs13259960; P = 0.0241). Moreover, SLEAR interacted with the RNA binding proteins interleukin enhancer binding factor 2, heterogeneous nuclear RNP F, and TATA-binding protein-associated factor 15, to form a complex for transcriptional activation of the downstream antiapoptotic genes. In addition, SLEAR regulated apoptosis of Jurkat cells in vitro, and its expression level was correlated with the degree of cell death in the peripheral blood of patients with SLE (r = 0.824, P = 2.15 × 10 ; n = 30).

Conclusion: These findings suggest a mechanism by which the risk variant at rs13259960 modulates SLEAR expression and confers a predisposition to SLE. Taken together, these results may give insights into the etiology of SLE.
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http://dx.doi.org/10.1002/art.41200DOI Listing
June 2020

Episo: quantitative estimation of RNA 5-methylcytosine at isoform level by high-throughput sequencing of RNA treated with bisulfite.

Bioinformatics 2020 04;36(7):2033-2039

CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.

Motivation: RNA 5-methylcytosine (m5C) is a type of post-transcriptional modification that may be involved in numerous biological processes and tumorigenesis. RNA m5C can be profiled at single-nucleotide resolution by high-throughput sequencing of RNA treated with bisulfite (RNA-BisSeq). However, the exploration of transcriptome-wide profile and potential function of m5C in splicing remains to be elucidated due to lack of isoform level m5C quantification tool.

Results: We developed a computational package to quantify Epitranscriptomal RNA m5C at the transcript isoform level (named Episo). Episo consists of three tools: mapper, quant and Bisulfitefq, for mapping, quantifying and simulating RNA-BisSeq data, respectively. The high accuracy of Episo was validated using an improved m5C-specific methylated RNA immunoprecipitation (meRIP) protocol, as well as a set of in silico experiments. By applying Episo to public human and mouse RNA-BisSeq data, we found that the RNA m5C is not evenly distributed among the transcript isoforms, implying the m5C may subject to be regulated at isoform level.

Availability And Implementation: Episo is released under the GNU GPLv3+ license. The resource code Episo is freely accessible from https://github.com/liujunfengtop/Episo (with Tophat/cufflink) and https://github.com/liujunfengtop/Episo/tree/master/Episo_Kallisto (with Kallisto).

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btz900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141862PMC
April 2020

Flexible and durable wood-based triboelectric nanogenerators for self-powered sensing in athletic big data analytics.

Nat Commun 2019 11 26;10(1):5147. Epub 2019 Nov 26.

CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Micro-nano Energy and Sensor, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, P. R. China.

In the new era of internet of things, big data collection and analysis based on widely distributed intelligent sensing technology is particularly important. Here, we report a flexible and durable wood-based triboelectric nanogenerator for self-powered sensing in athletic big data analytics. Based on a simple and effective strategy, natural wood can be converted into a high-performance triboelectric material with excellent mechanical properties, such as 7.5-fold enhancement in strength, superior flexibility, wear resistance and processability. The electrical output performance is also enhanced by more than 70% compared with natural wood. A self-powered falling point distribution statistical system and an edge ball judgement system are further developed to provide training guidance and real-time competition assistance for both athletes and referees. This work can not only expand the application area of the self-powered system to smart sport monitoring and assisting, but also promote the development of big data analytics in intelligent sports industry.
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http://dx.doi.org/10.1038/s41467-019-13166-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879608PMC
November 2019
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