Publications by authors named "Jianhua Zhou"

334 Publications

Expression and Clinical Significance of Lactate Dehydrogenase A in Colon Adenocarcinoma.

Front Oncol 2021 9;11:700795. Epub 2021 Jul 9.

Department of Pathology, Xiangya Hospital, Central South University, Changsha, China.

Lactate dehydrogenase A (LDHA) is an important glycolytic enzyme that promotes glycolysis and plays a crucial role in cancer cell invasion and immune infiltration. However, the relevance of LDHA in colon adenocarcinoma (COAD) remains unclear. In this study, we analyzed the correlation between the expression of LDHA and clinicopathological characteristics in COAD using immunohistochemistry analysis, and then used integrative bioinformatics analyses to further study the function and role of LDHA in COAD. We found that LDHA was highly expressed in COAD tissues compared with adjacent normal tissues, and that COAD patients with high LDHA expression levels showed poor survival. In addition, LDHA expression was closely associated with the immune infiltrating levels of CD8+ T cells, neutrophils, and dendritic cells. Our findings highlight the potential role of LDHA in the tumorigenesis and prognosis of COAD. Furthermore, our results indicate that COAD is a novel immune checkpoint in the diagnosis and treatment of COAD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.700795DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8300199PMC
July 2021

circRNA derived from CLSPN (circCLSPN) is an oncogene in human glioblastoma multiforme by regulating cell growth, migration and invasion via ceRNA pathway.

J Biosci 2021 ;46

Department of Neurosurgery, Renmin Hospital of Wuhan Caidian District, Wuhan, Hubei, China.

Glioblastoma multiforme (GBM) is the most aggressive and prevalent brain tumor in adults. The circRNA derived from CLSPN (hsa_circ_0011591, circCLSPN) is remarkably upregulated in GBM; however its functional role was uncovered yet. First, we examined expression of circCLSPN using GSE109569 database and RT-qPCR, and circCLSPN level was upregulated in human GBM tumor tissues and cells (A172 and LN18); moreover, circCLSPN showed a stable structure stability. Then, a series of loss-of-functional experiments were performed using CCK-8 assay, colony formation assay, flow cytometry, scratch wound assay, and transwell assay. Consequently, circCLSPN silencing suppressed cell viability, colony formation ability, cell cycle progression, migration, and invasion of A172 and LN18 cells , and promoted apoptosis rate. Allied with those were decreased B cell lymphoma-2 (Bcl-2), matrix metalloproteinase-2 (MMP2) and MMP9 expression, and elevated Bcl-2-associated X protein (Bax) level. According to dual-luciferase reporter assay and RNA pull-down assay, miR-370-3p was identified to be targeted and sponged by circCLSPN, and further targeted and negatively regulated USP39. Functionally, overexpressing miR-370-3p could mimic effects of circCLSPN interference. Rescue experiments revealed that blocking miR-370-3p could partially reverse the suppression of circCLSPN knockdown on cell growth, migration and invasion, and role of miR- 370-3p overexpression was abrogated by restoring USP39. , circCLSPN knockdown hindered tumor growth of LN18 cells by affecting miR-370-3p, USP39, MMP2 and MMP9 expression. In conclusion, circCLSPN elicited an oncogenic role in tumorigenesis and malignant progression of human GBM cells through circCLSPN-miR-370-3p-USP39 pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
January 2021

Author Correction: Deep learning radiomics can predict axillary lymph node status in early-stage breast cancer.

Nat Commun 2021 Jul 12;12(1):4370. Epub 2021 Jul 12.

Department of Ultrasound, Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-24605-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275735PMC
July 2021

Sensitive and rapid on-site detection of SARS-CoV-2 using a gold nanoparticle-based high-throughput platform coupled with CRISPR/Cas12-assisted RT-LAMP.

Sens Actuators B Chem 2021 Oct 6;345:130411. Epub 2021 Jul 6.

School of Biomedical Engineering, Sun Yat-sen University, Shenzhen, 518107, China.

The outbreak of corona virus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic. The high infectivity of SARS-CoV-2 highlights the need for sensitive, rapid and on-site diagnostic assays of SARS-CoV-2 with high-throughput testing capability for large-scale population screening. The current detection methods in clinical application need to operate in centralized labs. Though some on-site detection methods have been developed, few tests could be performed for high-throughput analysis. We here developed a gold nanoparticle-based visual assay that combines with CRISPR/Cas12a-assisted RT-LAMP, which is called Cas12a-assisted RT-LAMP/AuNP (CLAP) assay for rapid and sensitive detection of SARS-CoV-2. In optimal condition, we could detect down to 4 copies/μL of SARS-CoV-2 RNA in 40 min. by naked eye. The sequence-specific recognition character of CRISPR/Cas12a enables CLAP a superior specificity. More importantly, the CLAP is easy for operation that can be extended to high-throughput test by using a common microplate reader. The CLAP assay holds a great potential to be applied in airports, railway stations, or low-resource settings for screening of suspected people. To the best of our knowledge, this is the first AuNP-based colorimetric assay coupled with Cas12 and RT-LAMP for on-site diagnosis of COVID-19. We expect CLAP assay will improve the current COVID-19 screening efforts, and make contribution for control and mitigation of the pandemic.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.snb.2021.130411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257267PMC
October 2021

Reduced Red Mud as the Solar Absorber for Solar-Driven Water Evaporation and Vapor-Electricity Generation.

ACS Appl Mater Interfaces 2021 Jul 25;13(26):30556-30564. Epub 2021 Jun 25.

Department of Chemical Systems Engineering, Graduate School of Engineering, Nagoya University, Nagoya 4648603, Japan.

The emergent solar-driven water evaporation technology provides a reassuring scheme for red mud (RM) utilization in environment and materials science. With fewer restrictions on raw materials, wide availability of sheer quantity, and high complexity in chemical composition, the RM may be a promising candidate for solar absorbers. Here, we developed a novel solar absorber with reduced RM. It features favorable light absorption and photothermal conversion ability using biomass pyrolysis. When added to the polyvinyl alcohol and chitosan gel substrate, the light absorptance can reach 94.65%, while the corresponding evaporation rate is as high as 2.185 kg m h under an illumination density of 1 kW m. We further demonstrated its potential as an efficient solar absorber in the solar-driven water evaporation and the thermoelectric device to realize the stable and efficient coproduction of vapor and electricity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c05228DOI Listing
July 2021

ALKBH1-8 and FTO: Potential Therapeutic Targets and Prognostic Biomarkers in Lung Adenocarcinoma Pathogenesis.

Front Cell Dev Biol 2021 3;9:633927. Epub 2021 Jun 3.

Department of Pathology, Xiangya Hospital, Central South University, Changsha, China.

The AlkB family consists of Fe(II)- and α-ketoglutarate-dependent dioxygenases that can catalyze demethylation on a variety of substrates, such as RNA and DNA, subsequently affecting tumor progression and prognosis. However, their detailed functional roles in lung adenocarcinoma (LUAD) have not been clarified in a comprehensive manner. In this study, several bioinformatics databases, such as ONCOMINE, TIMER, and DiseaseMeth, were used to evaluate the expression profiles and prognostic significance of the AlkB family (ALKBH1-8 and FTO) in LUAD. The expression levels of ALKBH1/2/4/5/7/8 were significantly increased in LUAD tissues, while the expression levels of ALKBH3/6 and FTO were decreased. The main functions of differentially expressed AlkB homologs are related to the hematopoietic system and cell adhesion molecules. We also found that the expression profiles of the AlkB family are highly correlated with infiltrating immune cells (i.e., B cells, CD8 + T cells, CD4 + T cells, macrophages, neutrophils and dendritic cells). In addition, DNA methylation analysis indicated that the global methylation levels of ALKBH1/2/4/5/6/8 and FTO were decreased, while the global methylation levels of ALKBH3/7 were increased. In addition, the patients with upregulated ALKBH2 have significantly poor overall survival (OS) and post-progressive survival (PPS). Taken together, our work could provide insightful information about aberrant AlkB family members as potential biomarkers for the diagnostic and prognostic evaluation of LUAD. Especially, ALKBH2 could be served as a therapeutic candidate for treating LUAD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2021.633927DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209387PMC
June 2021

Vascularized Carotid Atherosclerotic Plaque Models for the Validation of Novel Methods of Quantifying Intraplaque Neovascularization.

J Am Soc Echocardiogr 2021 Jun 12. Epub 2021 Jun 12.

Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada; Department of Medicine, Cardiovascular Imaging Network at Queen's, Queen's University, Kingston, Ontario, Canada. Electronic address:

Background: Intraplaque neovascularization (IPN) in advanced lesions of the carotid artery has been linked to plaque progression and risk of rupture. Quantitative measurement of IPN may provide a more powerful tool for the detection of such "vulnerable" plaque than the current visual scoring method. The aim of this study was to develop a phantom platform of a neovascularized atherosclerotic plaque within a carotid artery to assess new methods of quantifying IPN.

Methods: Ninety-two synthetic plaque models with various IPN architectures representing different ranges of IPN scoring were created and assessed using contrast-enhanced ultrasound. Intraplaque neovascularization volume was calculated from contrast infiltration in B mode. The plaque models were used to develop a testing platform for IPN quantification. A neovascularized enhancement ratio (NER) was calculated using commercially available software. The plaque model NERs were then compared to human plaque NERs (n = 42) to assess score relationship. Parametric mapping of dynamic intensity over time was used to differentiate IPN from calcified plaque regions.

Results: A positive correlation between NER and IPN volume (rho = 0.45; P < .0001) was found in the plaque models. Enhancement of certain plaque model types showed that they resembled human plaques, with visual grade scores of 0 (NER mean difference = 1.05 ± SE 2.45; P = .67), 1 (NER mean difference = 0.22 ± SE 3.26; P = .95), and 2 (NER mean difference = -0.84 ± SE 3.33; P = .80). An optimal cutoff for NER (0.355) identified grade 2 human plaques with a sensitivity of 95% and specificity of 91%.

Conclusions: We developed a carotid artery model of neovascularized plaque and established a quantitative method for IPN using commercially available technology. We also developed an analysis method to quantify IPN in calcified plaques. This novel tool has the potential to improve clinical identification of vulnerable plaques, providing objective measures of IPN for cardiovascular risk assessment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.echo.2021.06.003DOI Listing
June 2021

Directing Group Enables Electrochemical Selectively -Bromination of Pyridines under Mild Conditions.

J Org Chem 2021 Jun 15. Epub 2021 Jun 15.

Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, People's Republic of China.

Without the use of catalysts and oxidants, a facile and sustainable electrochemical bromination protocol was developed. By introducing the directing groups, the regioselectivity of pyridine derivatives could be controlled at the -position utilizing the inexpensive and safe bromine salts at room temperature. A variety of brominated pyridine derivatives were obtained in 28-95% yields, and the reaction could be readily performed at a gram scale. By combining the installation and removing the directing group, the concept of -bromination of pyridines could be verified.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.joc.1c00923DOI Listing
June 2021

Identifying key areas of imbalanced supply and demand of ecosystem services at the urban agglomeration scale: A case study of the Fujian Delta in China.

Sci Total Environ 2021 Jun 5;791:148173. Epub 2021 Jun 5.

School of Architecture, Tianjin University, Tianjin 300072, China.

The coordinated supply and demand of ecosystem services (ESs) is important for ensuring regional sustainable development. However, research identifying key areas of imbalanced supply and demand of ESs at the urban agglomeration scale is limited. Therefore, in this study, using the Fujian Delta urban agglomeration of China as the research area, based on multi-source data, and analysis tools, such as ArcGIS, ENVI, and GeoDa, we constructed a research framework and indicator system for ESs supply and demand to determine the spatial change law, matching degree, and coupling coordination degree (CCD) of the ESs. On this basis, the key areas of imbalanced supply and demand of ESs were identified, and optimization strategies were proposed. The results showed that (1) there is obvious spatial heterogeneity between ESs supply and demand in the study area, and different degrees of spatial changes occurred with urbanization. Specifically, areas with large changes were concentrated in urban core areas and economic development zones. (2) The matching degree between ESs supply and demand is quite diverse and shows a trend of polarization. Under the influence of urbanization, some cities began facing ESs supply shortages. (3) Overall, the CCD between ESs supply and demand in the study area is in a state of mild incoordination, but with increasing urbanization, some cities have turned into a state of extreme incoordination. Our results indicate that the ESs supply and demand status in some cities at the urban agglomeration scale has become increasingly severe. Therefore, it is necessary to focus on certain "key areas" to formulate optimization strategies. For key areas with "low supply-high demand" and extreme incoordination, the population and land use intensity should be controlled to reduce the ESs demand level. Meanwhile, for key areas with "high supply-low demand" and extreme incoordination, the utilization efficiency of ecological resources should be improved to enhance the ESs supply capacity. The results of this study will help decision-makers optimize the relationship between ESs supply and demand in order to achieve the sustainable development of urban agglomeration.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.148173DOI Listing
June 2021

Validation of American College of Radiology Ovarian-Adnexal Reporting and Data System Ultrasound (O-RADS US): Analysis on 1054 adnexal masses.

Gynecol Oncol 2021 Jul 7;162(1):107-112. Epub 2021 May 7.

Department of Ultrasound, Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China. Electronic address:

Objective: To assess the diagnostic performance and inter-observer agreement of the American College of Radiology (ACR) Ovarian-Adnexal Reporting and Data System Ultrasound (O-RADS US).

Methods: From January 2016 to December 2018 a total of 1054 adnexal lesions in 1035 patients with pathologic results from two hospitals were retrospectively included. Each lesion was assigned to an O-RADS US category according to the criteria. Kappa (κ) statistics were applied to assess inter-observer agreement between a less experienced and an expert radiologist.

Results: Of the 1054 adnexal lesions, 750 were benign and 304 were malignant. The malignancy rates of O-RADS 5, O-RADS 4, O-RADS 3, and O-RADS 2 lesions were 89.57%, 34.46%, 1.10%, and 0.45% respectively. Area under the receiver operating characteristic curve was 0.960 (95% CI, 0.947-0.971). The optimal cutoff value for predicting malignancy was >O-RADS 3 with a sensitivity and specificity of 98.7% (95% CI, 0.964-0.996) and 83.2% (95% CI, 0.802-0.858) respectively. When sub-classifying multilocular cysts and smooth solid lesions in O-RADS 4 lesions as O-RADS 4a lesions and the rest cystic lesions with solid components as O-RADS 4b lesions, the malignancy rate were 17.02% and 42.57% respectively, which showed better risk stratification (P < 0.001). The inter-observer agreement between a less-experienced and an expert radiologist of O-RADS categorization was good (κ = 0.714).

Conclusions: The ACR O-RADS US provides effective malignancy risk stratification for adnexal lesions with high reliability for radiologists with different experience. Sub-grouping of O-RADS 4 lesions into two groups facilitated better stratification of the intermediate risk.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ygyno.2021.04.031DOI Listing
July 2021

Simvastatin intervention mitigates hypercholesterolemia-induced alveolar bone resorption in rats.

Exp Ther Med 2021 Jun 15;21(6):628. Epub 2021 Apr 15.

Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Shangdong University and Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, Shandong 250012, P.R. China.

Simvastatin promotes bone formation and increases bone mineral density in patients with hyperlipidemia and ameliorates hypercholesterolemia-induced microstructure changes in the jaw bone of animals. However, whether and how treatment with simvastatin can modulate the hypercholesterolemia-induced alveolar bone resorption is unclear. The present study aimed to examine the therapeutic efficacy and potential mechanisms of simvastatin application in hypercholesterolemia-induced alveolar bone resorption. The association between hyperlipidemia and alveolar bone resorption in 100 patients with periodontitis was examined. Additionally, male Sprague-Dawley rats were fed a standard rodent chow (NC) for 32 weeks or a high cholesterol diet (HCD) for 24 weeks. The HCD-fed rats were randomized, continually fed with HCD and treated with vehicle saline (HC) or simvastatin by gavage (5 mg/kg; SIM, n=10/group) for 8 weeks. The morphological changes to alveolar bone resorption in rats were analyzed by linear measurements. The relative levels of osteoprotegerin (OPG), receptor activator of nuclear factor-κB ligand RANKL, nuclear factor-κB (NF-κB), microtubule-associated protein 1 light chain 3 (LC3) and p62 in the alveolar bone tissues were determined by reverse transcription-quantitative PCR and/or immunohistochemistry. Sulcus bleeding index (SBI), clinical attachment loss (CAL), probing depth (PD) and the distance of cemantoenamel junction-alveolar bone crest (CEJ-ABC) in patients with hyperlipidemia were significantly greater than that in the controls (P<0.001). The levels of hyperlipidemia were positively correlated with the values of SBI, CAL, PD and CEJ-ABC in this population. Compared with the NC rats, higher levels of alveolar bone resorption, NF-κB expression, higher ratios of RANKL/OPG mRNA transcripts and LC3 to p62 expression were detected in the alveolar bone tissues of HC group. Simvastatin intervention significantly mitigated hypercholesterolemia-induced alveolar bone loss and RANKL mRNA transcription, but increased the ratios of LC3/p62 protein expression in the alveolar bone tissues of rats. Hyperlipidemia is associated with alveolar bone resorption and simvastatin treatment alleviated the hypercholesterolemia-related alveolar bone loss by down-regulating the NF-κB expression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/etm.2021.10060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082588PMC
June 2021

Piperlongumine inhibits the progression of osteosarcoma by downregulating the SOCS3/JAK2/STAT3 pathway via miR-30d-5p.

Life Sci 2021 Jul 15;277:119501. Epub 2021 Apr 15.

Department of Spine Surgery, People's Hospital of Longhua, Affiliated Hospital of Southern Medical University, Guangdong 518000, Shenzhen, China. Electronic address:

Aims: The present study evaluated the functions of Piperlongumine (PL) in osteosarcoma (OS) cell growth and metastasis both in vitro and in vivo.

Main Methods: MTT assay was conducted to test the cytotoxic effects of PL on the human osteoblasts line HFOB1.19 and the human normal chondrocyte line C28/I2T. FITC-Annexin V and propidium iodide (PI) were used to examine cell apoptosis. The migration, invasion and relative epithelial-mesenchymal transition were examined by Transwell assay and Western blotting. Reverse transcription-quantitative PCR (RT-qPCR) was performed to analyze the cytokine signaling 3 (SOCS3) mRNA expression. TargetScan database was used to predict the target of SOCS3. The binding association between miR-30d-5p and SOCS3 in U2OS and MG63 cells was evaluated by the dual-luciferase reporter assay. A xenograft model was constructed to evaluate the effect of PL on OS cell growth in vivo.

Key Findings: The results revealed that PL inhibited the growth, migration, invasion, epithelial-mesenchymal transition, and promoted the apoptosis of OS cells dose-dependently. In addition, PL upregulated the protein levels of suppressor of SOCS3, while it inactivated the JAK2/STAT3 pathway, which was accompanied by a decreased level of microRNA (miR)-30d-5p. Furthermore, SOCS3was confirmed as a novel target of miR-30d-5p. Overexpression of miR-30d-5p not only led to decreased expression of SOCS3, but also dampened the antitumor effect of PL on OS.

Significance: The present data demonstrated that PL inhibited the progression of OS via downregulation of the SOCS3-mediated JAK2/STAT3 pathway by inhibiting miR-30d-5p.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2021.119501DOI Listing
July 2021

Relations among positivity, positive affect in school, and learning flow in elementary school students: A longitudinal mediation model.

Br J Educ Psychol 2021 Apr 9:e12418. Epub 2021 Apr 9.

School of Psychology, South China Normal University, Guangzhou, China.

Background: Learning flow is an optimal learning experience representing full engagement in one's studies. The belief-affect-engagement model and control-value theory suggest that positivity would be a motivator of learning flow, while positive affect in school would be a mediator of the relation between positivity and learning flow.

Aims: The current research aimed to examine (1) the longitudinal relations among positivity, positive affect in school, and learning flow, and (2) the mediational role of positive affect in school between positivity and learning flow.

Sample And Method: A sample of 4681 Chinese elementary school students (44.9% girls; M  = 9.87 years, SD = 0.70 at Time 1) completed reliable measures for each construct on four occasions across 2 years, using 6-month intervals. Structural equation modelling was used for examining study hypotheses.

Results: After controlling for gender, age, and family socioeconomic status, the results showed that (1) positivity, positive affect in school, and learning flow reciprocally facilitated each other directly; (2) positive affect in school mediated the relation between positivity and later learning flow, as well as the relation between learning flow and later positivity.

Conclusions: These findings revealed that positivity, positive affect in school, and learning flow form a complex, dynamic system, suggesting that school professionals should consider monitoring and developing interventions based upon these variables as early as elementary school.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/bjep.12418DOI Listing
April 2021

The Emerging Landscape of Long Non-Coding RNAs in Colorectal Cancer Metastasis.

Front Oncol 2021 25;11:641343. Epub 2021 Feb 25.

Department of Pathology, Xiangya Hospital, Central South University, Changsha, China.

Colorectal cancer (CRC) is one of the most common gastrointestinal cancers, with extremely high rates of morbidity and mortality. The main cause of death in CRC is distant metastasis; it affects patient prognosis and survival and is one of the key challenges in the treatment of CRC. Long non-coding RNAs (lncRNAs) are a group of non-coding RNA molecules with more than 200 nucleotides. Abnormal lncRNA expression is closely related to the occurrence and progression of several diseases, including cancer. Recent studies have shown that numerous lncRNAs play pivotal roles in the CRC metastasis, and reversing the expression of these lncRNAs through artificial means can reduce the malignant phenotype of metastatic CRC to some extent. This review summarizes the major mechanisms of lncRNAs in CRC metastasis and proposes lncRNAs as potential therapeutic targets for CRC and molecular markers for early diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.641343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947863PMC
February 2021

The Significance of HOXC11 and LSH in Survival Prediction in Gastric Adenocarcinoma.

Onco Targets Ther 2021 1;14:1517-1529. Epub 2021 Mar 1.

Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education; Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan, 410078, People's Republic of China.

Purpose: Gastric adenocarcinoma is one of the most important causes of cancer death and lacks effective treatment. Eighty-four gastric adenocarcinoma tissue samples along with the clinical information were collected. After analyzing the expression of HOXC11 and LSH in the gastric adenocarcinoma tissues, we explored the prognosis of patients and its correlation with clinical characteristics. Both HOXC11 and LSH were over-expressed in MKN-45 cell lines to verify the effect of high expression of HOXC11 and LSH on GAC.

Methods: The expression of HOXC11 and LSH in 84 cases with gastric adenocarcinoma (GAC) was detected via immunohistochemistry, including 17 cases in stage I, 7 cases in stage II, 27 cases in stage III and 33 cases in stage IV. The expression levels of HOXC11 and LSH, and the clinicopathological characteristics of the samples, were also studied. Cell proliferation, migration, cell cycle and apoptosis assays were utilized for demonstrating malignancy of HOXC11 and LSH over-expressed cells.

Results: Among 84 GAC pathological samples, 12 high HOXC11 expression, and 72 showed low expression; 54.8% (46/84) high LSH expression, and 45.2% (38/84) exhibited low expression. Survival analysis of the Kaplan-Meier plotter gastric cancer datasets showed that subjects with low expression of HOXC11 and LSH had a longer survival time, with a median survival time of 40.2 and 36.4 months, while the subjects with high HOXC11 and LSH expression were only 20.5 and 10 months, respectively. Meanwhile, HOXC11 and LSH over-expressed cells showed a stronger proliferous and migratory ability, and a sped up cell cycle.

Conclusion: The high expression level of HOXC11 and LSH both manifested the poor survival prognosis of GAC patients, and more pronounced malignant phenotype in GAC cells indicated that HOXC11 and LSH can be a strong predictive factor of inferior disease-free survival. From this, we can consider that HOXC11 and LSH both have significant status in GAC stage and survival prediction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/OTT.S273195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935444PMC
March 2021

Clinical Application of Liver Imaging Reporting and Data System for Characterizing Liver Neoplasms: A Meta-Analysis.

Diagnostics (Basel) 2021 Feb 17;11(2). Epub 2021 Feb 17.

Department of Ultrasound, Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou 510060, China.

The Liver Imaging Reporting and Data System (LI-RADS) is a comprehensive system for standardizing liver imaging in patients at risk of developing hepatocellular carcinoma (HCC). We aimed to determine the diagnostic performance of LI-RADS category 5 (LR5) for diagnosing HCC and LI-RADS category M (LRM) for characterizing other non-HCC malignancies (OM) using contrast-enhanced ultrasound (CEUS) and computed tomography (CT)/magnetic resonance imaging (MRI). Multiple databases were searched for articles evaluating the diagnostic accuracy of CEUS LI-RADS and/or CT/MRI LI-RADS. A random-effects model was adopted to synthesize the summary estimates of the diagnostic accuracy of LR5 for diagnosing HCC and LRM for characterizing OM using CEUS and CT/MRI. The pooled sensitivity and specificity of CEUS LR5 for the diagnosis of HCC were 69% and 93%, respectively. The pooled sensitivity was 67% and the specificity, 93% of CT/MRI LR5 for HCC diagnosis. There was no significant difference between the overall diagnostic accuracy for HCC diagnosis of CEUS LR5 and that of CT/MRI LR5 in terms of diagnostic odds ratio (DOR) ( = 0.55). The sensitivity was 84% with a specificity of 90% in the CEUS LRM for characterizing OM, while the sensitivity and specificity of CT/MRI LRM for characterizing OM was 63% and 95%. The DOR of CEUS LRM for characterizing OM was higher than that of CT/MRI LRM without significant difference (50.59 vs. 36.06, = 0.34). This meta-analysis indicated that CEUS LI-RADS is qualified to characterize HCC and OM and may provide complementary information on liver nodules to CT/MRI LI-RADS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/diagnostics11020323DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921912PMC
February 2021

Ultrasound-visualized, site-specific vascular embolization using magnetic protein microcapsules.

J Mater Chem B 2021 03;9(10):2407-2416

School of Biomedical Engineering, Sun Yat-sen University, Guangzhou 510006, China.

Imaging-guided vascular embolization is frequently performed on patients with advanced hepatocellular carcinoma (HCC) to alleviate symptoms and extend their survival time. Current operation procedures are not only painful for patients, but are also inaccurate in tumor targeting after the release of embolic agents from the catheter, leading to injury to healthy tissues simultaneously. In this study, we developed an ultrasound-visualized, site-specific vascular embolization strategy with magnetic protein microcapsules (MPMs). MPMs were fabricated using a rapid emulsification method, giving it a smooth surface and a core-shell structure. Their diameters could be controlled within 10 μm, allowing them to pass through capillaries. The core-shell structure and loading of magnetic Fe3O4 endowed MPMs with good contrast under ultrasound imaging and magnetically inducible targeting properties, as well as aggregation response even under flowing conditions. In vitro cytotoxicity and hemolysis evaluation demonstrated good biocompatibility of the MPMs. Furthermore, mock embolization showed that cell death could be induced by aggregation of the MPMs. Such a combination of real-time monitoring using ultrasound and control on targeted vascular embolization might be a breakthrough in the treatment of advanced HCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0tb02715dDOI Listing
March 2021

Effect of Quxie capsule in patients with colorectal cancer: A systematic review and meta-analysis.

Medicine (Baltimore) 2021 Feb;100(7):e24322

Department of Traditional Chinese Medicine, Shanghai Eighth People's Hospital.

Objective: To investigate whether the Quxie capsule can decrease relapse, metastasis, and symptoms, as well as alleviate the side effects in colorectal cancer (CRC) patients.

Methods: A comprehensive literature search of multiple databases was performed. Two reviewers independently selected trials that assessed the relapse-metastasis rate, degree of symptoms, and side effects of Quxie capsule for CRC. The meta-analysis was performed using Review Manager 5.3.

Results: This meta-analysis included 6 studies, with a total of 408 cases. The quality of the included studies was generally low, with only 1 trial of high quality. A statistically significant difference was observed in the relapse-metastasis rate between the Quxie capsule and control groups after 2-years follow-up (n = 185, relative risk (RR) = 0.13, 95% confidence interval (CI) 0.04-0.46; P = .002). The Quxie capsule was found to reduce the traditional Chinese medicine symptom score as compared to the control (n = 208, weighted mean differences (WMD) = -4.15, 95% CI -7.30 to -1.00; P = .010), while it showed no significant improvement in the Karnofsky Performance Status score (n = 138, WMD = 5.05; 95% CI -2.95 to 13.04; P = .22). There was no difference in adverse events between the 2 groups (P = .66).

Conclusion: This systematic review and meta-analysis showed no clear superiority of Quxie capsule for CRC patients receiving chemotherapy. The effect of Quxie capsule in CRC patients should be examined by high quality, large sample size, multi-center RCTs, with longer follow-up.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000024322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7899823PMC
February 2021

EBV as a high infection risk factor promotes RASSF10 methylation and induces cell proliferation in EBV-associated gastric cancer.

Biochem Biophys Res Commun 2021 Apr 12;547:1-8. Epub 2021 Feb 12.

Xiangya Hospital, Department of Pathology, Cancer Research Institute, School of Basic Medical Science, Central South University, Changsha, Hunan, 410008, China; Hunan Key Laboratory of Nonresolving Inflammation and Cancer, NHC Key Laboratory of Carcinogenesis, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Changsha, Hunan, China. Electronic address:

Epstein-Barr virus (EBV) is the first identified human tumor-related DNA virus, and has a high infection among people worldwide. Recent studies have showed that nearly 10% of gastric cancers have shown EBV infection and this kind of gastric cancer has been identified as a new subtype: EBV associated Gastric cancer (EBVaGC). Furthermore, it has been reported that tumor related genes in the EBVaGC showed frequent methylation modifications compared to those in the EBV negative gastric cancer (EBVnGC). To fully understand the role of EBV in EBVaGC, we analyzed and found that 16.67% of gastric carcinoma samples showed positive EBER1 signals. Mechanically, EBV-encoded Latent membrane protein 1 (LMP1) inhibited the expression of RASSF10, and promoted tumorigenesis by recruiting DNMT1 and inducing the DNA methylation of RASSF10. Altogether, it allows us a better understanding of the possible mechanism of EBV-induced gene hypermethylation in gastric cancer genome. Targeting EBV-induced DNA methylation is a potential therapeutic modality of EBVaGC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrc.2021.02.014DOI Listing
April 2021

Metaoptronic Multiplexed Interface for Probing Bioentity Behaviors.

Nano Lett 2021 03 1;21(6):2681-2689. Epub 2021 Feb 1.

Key Laboratory of Sensing Technology and Biomedical Instruments of Guangdong Province, School of Biomedical Engineering, Sun Yat-sen University, Guangzhou 510275, China.

Biointerface sensors have brought about remarkable advances in modern biomedicine. To accurately monitor bioentity's behaviors, biointerface sensors need to capture three main types of information, which are the electric, spectroscopic, and morphologic signals. Simultaneously obtaining these three types of information is of critical importance in the development of future biosensor, which is still not possible in the existing biosensors. Herein, by synergizing metamaterials, optical, and electronic sensing designs, we proposed the metaoptronic multiplexed interface (MMI) and built a MMI biosensor which can collectively record electric, spectroscopic, and morphologic information on bioentities. The MMI biosensor enables the real-time triple-monitoring of cellular dynamics and opens up the possibility for powerlessly monitoring ocular dryness. Our findings not only demonstrate an advanced multiplexed biointerface sensor with integrated capacities but also help to identify a uniquely significant arena for the nanomaterials, meta-optics, and nanotechnologies to play their roles in a complementary manner.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.nanolett.0c04067DOI Listing
March 2021

Value of dual-phase, contrast-enhanced CT combined with ultrasound for the diagnosis of metastasis to central lymph nodes in patients with papillary thyroid cancer.

Clin Imaging 2021 Jul 12;75:5-11. Epub 2021 Jan 12.

Department of Ultrasound, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou 510060, PR China. Electronic address:

Aims: To evaluate the diagnostic performance of computed tomography combined with ultrasound (CT/US) in metastatic central lymph nodes (CLNs) compared with US in patients with papillary thyroid cancer (PTC).

Material And Methods: Six-hundred patients with surgically proven PTC who underwent both US and CT examination before CLN dissection were evaluated retrospectively. All cases were divided into four subgroups according to the tumor size and number. Diagnostic performances of US, CT and CT/US were evaluated.

Results: Among 600 patients with CLN dissection, CT/US showed higher sensitivity (89.10%) and accuracy (83.00%) than US alone (76.06%, 76.50%). In the subgroup of solitary non-microcarcinomas, the AUC of CT/US was significantly higher than that for US alone (0.827 vs. 0.722, P < 0.05). For the subgroup of solitary or multiple microcarcinomas, the performance efficiency of CT had no obvious advantage over that of US (0.698 vs. 0.740, 0.798 vs. 0.802, P > 0.05). For the subgroup of multiple non-microcarcinomas, CT, US and US/CT had high diagnostic efficacy (AUC > 0.8, Accuracy >80%. P > 0.05), and there was no significant difference between them.

Conclusions: In the subgroup of solitary non-microcarcinoma, CT combined with US provided better diagnostic efficacy, and for those cases, complementary CT examination was recommended. In other subgroups, the diagnostic efficacy of US/CT was similar to that of US alone, and there was no significant benefit from additional CT examination.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.clinimag.2021.01.008DOI Listing
July 2021

Self-Cleaning Integrative Aerogel for Stable Solar-Assisted Desalination.

Glob Chall 2021 Jan 16;5(1):2000063. Epub 2020 Dec 16.

Guangxi Key Laboratory of Information Material Guangxi Collaborative Innovation Center of Structure and Property for New Energy and Materials School of Material Science and Engineering Guilin University of Electronic Technology Guilin 541004 China.

The solar-assisted desalination generator (SADG) shows great potential for solving water scarcity problems. However, salt precipitation and accumulation is still a challenge for SADG, which slows down solar steam generation performance of evaporator during operation. Here, a facile integrative evaporator featuring stable and high evaporation performance breaks this bottleneck. By using a rational design in which amorphous carbon particles are evenly composited within the porous chitosan aerogel, the evaporator not only integrates excellent light absorption, heat management, and water transportation abilities but also endows a large vapor escape space. Upon desalination, salt concentration ingredients between carbon particles and chitosan membranes can be quickly balanced by water transport in interconnected chitosan chains, and thus salt precipiation on the evaporator surface would be avoided. Compared to other salt-rejection evaporators, the integrative evaporator can operate in 3.5 wt% brine for 60 days without salt precipiation and exhibits a stable evaporation rate (1.70 kg m h), indicating its potential for practical applications in seawater desalination and the harvest of clean drinking water.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/gch2.202000063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788587PMC
January 2021

Reciprocating-flowing on-a-chip enables ultra-fast immunobinding for multiplexed rapid ELISA detection of SARS-CoV-2 antibody.

Biosens Bioelectron 2021 Mar 29;176:112920. Epub 2020 Dec 29.

Key Laboratory of Sensing Technology and Biomedical Instruments of Guangdong Province, School of Biomedical Engineering, Sun Yat-sen University, Guangzhou, 510275, China. Electronic address:

The worldwide epidemic of novel coronavirus disease (COVID-19) has led to a strong demand for highly efficient immunobinding to achieve rapid and accurate on-site detection of SARS-CoV-2 antibodies. However, hour-scale time-consumption is usually required to ensure the adequacy of immunobinding on expensive large instruments in hospitals, and the common false negative or positive results often occur in rapid on-site immunoassay (e.g. immunochromatography). We solved this dilemma by presenting a reciprocating-flowing immunobinding (RF-immunobinding) strategy. RF-immunobinding enabled the antibodies in fluid contacting with the corresponding immobilized antigens on substrate repeatedly during continuous reciprocating-flowing, to achieve adequate immunobinding within 60 s. This strategy was further developed into an immunoassay method for the serological detection of 13 suspected COVID-19 patients. We obtained a 100% true negative and true positive rate and a limit of quantification (LOQ) of 4.14 pg/mL. Our strategy also can be a potential support for other areas related to immunorecognition, such as proteomics, immunopharmacology and immunohistochemistry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2020.112920DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7834412PMC
March 2021

Microfluidic High-Throughput Platforms for Discovery of Novel Materials.

Nanomaterials (Basel) 2020 Dec 15;10(12). Epub 2020 Dec 15.

Key Laboratory of Sensing Technology and Biomedical Instruments of Guangdong Province, School of Biomedical Engineering, Sun Yat-Sen University, Guangzhou 510006, China.

High-throughput screening is a potent technique to accelerate the discovery and development of new materials. By performing massive synthesis and characterization processes in parallel, it can rapidly discover materials with desired components, structures and functions. Among the various approaches for high-throughput screening, microfluidic platforms have attracted increasing attention. Compared with many current strategies that are generally based on robotic dispensers and automatic microplates, microfluidic platforms can significantly increase the throughput and reduce the consumption of reagents by several orders of magnitude. In this review, we first introduce current advances of the two types of microfluidic high-throughput platforms based on microarrays and microdroplets, respectively. Then the utilization of these platforms for screening different types of materials, including inorganic metals, metal alloys and organic polymers are described in detail. Finally, the challenges and opportunities in this promising field are critically discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano10122514DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7765132PMC
December 2020

Application of artificial intelligence to the diagnosis and therapy of colorectal cancer.

Am J Cancer Res 2020 1;10(11):3575-3598. Epub 2020 Nov 1.

Department of Pathology, Xiangya Hospital, Central South University Changsha 410008, Hunan, China.

Artificial intelligence (AI) is a relatively new branch of computer science involving many disciplines and technologies, including robotics, speech recognition, natural language and image recognition or processing, and machine learning. Recently, AI has been widely applied in the medical field. The effective combination of AI and big data can provide convenient and efficient medical services for patients. Colorectal cancer (CRC) is a common type of gastrointestinal cancer. The early diagnosis and treatment of CRC are key factors affecting its prognosis. This review summarizes the research progress and clinical application value of AI in the investigation, early diagnosis, treatment, and prognosis of CRC, to provide a comprehensive theoretical basis for AI as a promising diagnostic and treatment tool for CRC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716173PMC
November 2020

Stretchable, Healable, and Degradable Soft Ionic Microdevices Based on Multifunctional Soaking-Toughened Dual-Dynamic-Network Organohydrogel Electrolytes.

ACS Appl Mater Interfaces 2020 Dec 4;12(50):56393-56402. Epub 2020 Dec 4.

School of Materials Science and Engineering, and Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Sun Yat-sen University, Guangzhou 510275, P. R. China.

Electronic materials and devices that can mimic biological systems featured with elasticity, toughness, self-healing, degradability, and environmental friendliness drive the technological developments in fields spanning from bioelectronics, biomedical diagnosis and therapy, electronic skin, and soft robotics to Internet-of-Things with "green" electronics. Among them, ionic devices based on gel electrolytes have emerged as attractive candidates for biomimetic systems. Herein, we presented a straightforward approach to demonstrate soft ionic microdevices based on a versatile organohydrogel platform acting as both a free-standing, stretchable, adhesive, healable, and entirely degradable support and a highly conductive, dehydration- and freezing-tolerant electrolyte. This is achieved by forming a gelatin/ferric-ion-cross-linked polyacrylic acid (GEL/PAA) dual dynamic supramolecular network followed by soaking into a NaCl glycerol/water solution to further toughen the gelatin network via solvent displacement, thus obtaining a high toughness of 1.34 MJ·cm and a high ionic conductivity (>7 mS·cm). Highly stretchable and multifunctional ionic microdevices are then fabricated based on the organohydrogel electrolytes by simple transfer printing of carbon-based microelectrodes onto the prestretched gel surface. Proof-of-concept microdevices including resistive strain sensors and microsupercapacitors are demonstrated, which displayed outstanding stretchability to 300% strain, resistance to dehydration for >6 months, autonomous self-healing, and rapid room-temperature degradation within hours. The present material design and fabrication approach for the organohydrogel-based ionic microdevices will provide promising scope for life-like and sustainable electronic systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c14472DOI Listing
December 2020

How to Be Aggressive from Virtual to Reality? Revisiting the Violent Video Games Exposure-Aggression Association and the Mediating Mechanisms.

Cyberpsychol Behav Soc Netw 2021 Jan 18;24(1):56-62. Epub 2020 Nov 18.

School of Psychology, South China Normal University, Guangzhou, People's Republic of China.

Few studies have simultaneously examined the underlying mechanisms of the link between violent video games exposure (VVGE) and aggression (as proposed by different theories) to examine how they explain the relationship between the concepts as independent-dependent variables. This study used a multi-informant design to examine the relationship between VVGE and the functions (proactive and reactive) of aggressive behaviors by comparing three mediating mechanisms: anger, moral disengagement, and cognitive impulsivity. The sample consisted of 2,095 Chinese children and adolescences (48.9 percent girls;  = 11.12 years,  = 1.70) and their mothers. After controlling for age, gender, socioeconomic status, child maltreatment, and problematic traits, structural equation modeling indicated that anger and moral disengagement play mediating roles between VVGE and proactive and reactive aggression, but cognitive impulsivity only plays a mediating role between VVGE and reactive aggression. The discussion emphasizes the importance of creating prevention programs for anger, moral disengagement, and cognitive impulsivity to break the VVGE-to-aggression cycle, and provides suggestions for future research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/cyber.2019.0762DOI Listing
January 2021

DEPDC1B is a tumor promotor in development of bladder cancer through targeting SHC1.

Cell Death Dis 2020 11 17;11(11):986. Epub 2020 Nov 17.

Department of Urology, Peking University People's Hospital, Beijing, China.

Bladder cancer is one of the most commonly diagnosed malignant tumors in the urinary system and causes a massive cancer-related death. DEPDC1B is a DEP domain-containing protein that has been found to be associated with a variety of human cancers. This study aimed to explore the role and mechanism of DEPDC1B in the development of bladder cancer. The analysis of clinical specimens revealed the upregulated expression of DEPDC1B in bladder cancer, which was positively related to tumor grade. In vitro and in vivo studies showed that DEPDC1B knockdown could inhibit the growth of bladder cancer cells or xenografts in mice. The suppression of bladder cancer by DEPDC1B was executed through inhibiting cell proliferation, cell migration, and promoting cell apoptosis. Moreover, a mechanistic study found that SHC1 may be an important route through which DEPDC1B regulates the development of bladder cancer. Knockdown of SHC1 in DEPDC1B-overexpressed cancer cells could abolish the promotion effects induced by DEPDC1B. In conclusion, DEPDC1B was identified as a key regulator in the development of bladder cancer, which may be used as a potential therapeutic target in the treatment of bladder cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41419-020-03190-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672062PMC
November 2020

Radiofrequency ablation of benign thyroid nodules: recommendations from the Asian Conference on Tumor Ablation Task Force.

Ultrasonography 2021 Jan 8;40(1):75-82. Epub 2020 Sep 8.

Department of Ultrasound, Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China.

Radiofrequency ablation (RFA) is a thermal ablation technique widely used for the management of benign thyroid nodules. To date, five academic societies in various countries have reported clinical practice guidelines, opinion statements, or recommendations regarding the use of thyroid RFA. However, despite some similarities, there are also differences among the guidelines, and a consensus is required regarding safe and effective treatment in Asian countries. Therefore, a task force was organized by the guideline committee of the Asian Conference on Tumor Ablation with the goal of devising recommendations for the clinical use of thyroid RFA. The recommendations in this article are based on a comprehensive analysis of the current literature and the consensus opinion of the task force members.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.14366/usg.20112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758103PMC
January 2021

Core Needle Biopsy Targeting the Viable Area of Deep-Sited Dominant Lesion Verified by Color Doppler and/or Contrast-Enhanced Ultrasound Contribute to the Actionable Diagnosis of the Patients Suspicious of Lymphoma.

Front Oncol 2020 7;10:500153. Epub 2020 Oct 7.

Department of Thoracic Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China.

Background: Inadequate accuracy of ultrasound-guided core needle biopsy (US-CNB) urges further improvement for the diagnosis and management of lymphoma to meet with the practitioners' increased reliance on this mini-invasive approach.

Methods: Data related to US-CNB of the deep-sited dominant lesions suspicious of lymphoma detected by computer tomography or positron-emission tomography/computer tomography for eligibility assessment of three prospective clinical trials were collected in advance. A retrospective analysis of the prospective data collection was performed, in which Viable-targeting US-CNB that Color Doppler flow imaging (CDFI) and/or contrast enhanced ultrasound (CEUS) were employed to select viable area for biopsy target compared with Routine US-CNB that routine procedure of evaluation and guidance using gray-scale ultrasound with CDFI in terms of the yield of clinically actionable diagnosis and safety, and determinants for the successful US-CNB that established an actionable diagnosis were explored. The establishment of final diagnosis was based on surgical pathology or medical response to therapy with follow-up at least 6 months.

Results: A total of 245 patients underwent Routine US-CNB ( = 120) or Viable-targeting US-CNB ( = 125), of which 91 (91/120, 75.8%) and 112 (112/125, 89.6%) were revealed with actionable diagnoses, respectively ( = 0.004, OR 0.846, 95% CI: 0.753-0.952). And 239 patients established final diagnoses. Diagnostic yields of actionable diagnosis according to the final diagnoses were 78.4% (91/116) and 91.1% (112/123) ( = 0.006, OR 0.554, 95% CI: 0.333-0.920), 82.6% (90/109) and 92.5% (111/120) for malignancy, 84.0% (84/100) and 91.8% (101/110) for lymphoma, 85.1% (80/94) and 92.3% (96/104) for Non-Hodgkin Lymphoma, 66.7% (4/6) and 83.3% (5/6) for Hodgkin Lymphoma in Routine and Viable-targeting CNB groups, respectively. No major complications were observed. Dominant lesions with actionable diagnosis in US-CNB were with higher FDG-avid Standardized Uptake Value. Binomial logistic regression revealed that actionable diagnosis of US-CNB was correlated with group and ancillary studies.

Conclusion: Viable-Targeting US-CNB was superior to routine US-CNB in term of the yield of actionable diagnosis for deep-sited dominant lesions suspicious of lymphoma, which demonstrated a potential to be the initial approach in this setting.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2020.500153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7577120PMC
October 2020
-->