Publications by authors named "Jianhua Zhang"

1,164 Publications

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Polymers for Improved Delivery of Iodinated Contrast Agents.

ACS Biomater Sci Eng 2021 Dec 1. Epub 2021 Dec 1.

Department of Polymer Science and Engineering, Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China.

X-ray computed tomography (CT), as one of the most widely used noninvasive imaging modalities, can provide three-dimensional anatomic details with high resolution, which plays a key role in disease diagnosis and treatment assessment. However, although they are the most prevalent and FDA-approved contrast agents, iodinated water-soluble molecules still face some challenges in clinical applications, such as fast clearance, serious adverse effects, nonspecific distribution, and low sensitivity. Because of their high biocompatibility, tunable designability, controllable biodegradation, facile synthesis, and modification capability, the polymers have demonstrated great potential for efficient delivery of iodinated contrast agents (ICAs). Herein, we comprehensively summarized the applications of multifunctional polymeric materials for ICA delivery in terms of increasing circulation time, decreasing nephrotoxicity, and improving the specificity and sensitivity of ICAs for CT imaging. We mainly focused on various iodinated polymers from the aspects of preparation, functionalization, and application in medical diagnosis. Future perspectives for achieving better imaging and clinical translation are also discussed to motivate new technologies and solutions.
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http://dx.doi.org/10.1021/acsbiomaterials.1c01082DOI Listing
December 2021

Polydopamine antibacterial materials.

Mater Horiz 2021 Jun 15;8(6):1618-1633. Epub 2021 Feb 15.

College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, Sichuan 610065, China.

Recently, the development of polydopamine (PDA) has demonstrated numerous excellent performances in free radical scavenging, UV shielding, photothermal conversion, and biocompatibility. These unique properties enable PDA to be widely used as efficient antibacterial materials for various applications. Accordingly, PDA antibacterial materials mainly include free-standing PDA materials and PDA-based composite materials. In this review, an overview of PDA antibacterial materials is provided to summarize these two types of antibacterial materials in detail, including the fabrication strategies and antibacterial mechanisms. The future development and challenges of PDA in this field are also presented. It is hoped that this review will provide an insight into the future development of antibacterial functional materials based on PDA.
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http://dx.doi.org/10.1039/d0mh01985bDOI Listing
June 2021

Surface Integrity and Friction Performance of Brass H62 Textured by One-Dimensional Ultrasonic Vibration-Assisted Turning.

Micromachines (Basel) 2021 Nov 14;12(11). Epub 2021 Nov 14.

School of Mechanical Engineering, Shandong University of Technology, Zibo 255000, China.

The processing method, one-dimensional ultrasonic vibration-assisted turning (1D UVAT), is a potential and efficient way for fabricating a micro-textured surface. This paper aims at exploring the surface integrity and friction performance of brass H62 textured by the 1D UVAT. Four micro-textured surfaces with a specific distribution, size, and shape of dimples were fabricated by optimizing processing parameters, and the corresponding surface topography, subsurface microstructure, and surface roughness were observed and analyzed. A series of friction tests were carried out under oil-lubricating conditions to research the friction performance of micro-textured surfaces. The results show that the reason for the deviation between theoretical and experimental values of dimple depth was further revealed by observing the corresponding subsurface microstructure. The surface roughness of the micro-textured surfaces prepared is related to the number of micro-dimples per unit area and dimple size, which is greater than the surface generated by conventional turning. Compared with the polished surface and micro-grooved surface, the micro-textured surfaces have better friction performance with a lower frictional coefficient (COF) and wear degree. For the micro-textured surface fabricated by 1D UVAT, the number of micro-dimples per unit area has a great effect on the friction performance, and choosing a larger number is more conducive to improving the friction performance under the oil-lubricating condition. Consequently, this study proves that the proposed 1D UVAT can be a feasible candidate for preparing a micro-textured surface with better tribological property.
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http://dx.doi.org/10.3390/mi12111398DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622952PMC
November 2021

Manifold Feature Fusion with Dynamical Feature Selection for Cross-Subject Emotion Recognition.

Brain Sci 2021 Oct 23;11(11). Epub 2021 Oct 23.

OsloMet Artificial Intelligence Lab, Department of Computer Science, Oslo Metropolitan University, N-0130 Oslo, Norway.

Affective computing systems can decode cortical activities to facilitate emotional human-computer interaction. However, personalities exist in neurophysiological responses among different users of the brain-computer interface leads to a difficulty for designing a generic emotion recognizer that is adaptable to a novel individual. It thus brings an obstacle to achieve cross-subject emotion recognition (ER). To tackle this issue, in this study we propose a novel feature selection method, manifold feature fusion and dynamical feature selection (MF-DFS), under transfer learning principle to determine generalizable features that are stably sensitive to emotional variations. The MF-DFS framework takes the advantages of local geometrical information feature selection, domain adaptation based manifold learning, and dynamical feature selection to enhance the accuracy of the ER system. Based on three public databases, DEAP, MAHNOB-HCI and SEED, the performance of the MF-DFS is validated according to the leave-one-subject-out paradigm under two types of electroencephalography features. By defining three emotional classes of each affective dimension, the accuracy of the MF-DFS-based ER classifier is achieved at 0.50-0.48 (DEAP) and 0.46-0.50 (MAHNOBHCI) for arousal and valence emotional dimensions, respectively. For the SEED database, it achieves 0.40 for the valence dimension. The corresponding accuracy is significantly superior to several classical feature selection methods on multiple machine learning models.
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http://dx.doi.org/10.3390/brainsci11111392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8615971PMC
October 2021

LINC01087 indicates a poor prognosis of glioma patients with preoperative MRI.

Funct Integr Genomics 2021 Nov 24. Epub 2021 Nov 24.

Department of Gynecology, Hainan General Hospital, Affiliated Hainan Hospital of Hainan Medical College, No.19 Xiuhua Road, Xiuying District, Haikou, 570311, Hainan, China.

Long intergenic non-coding RNA 01,087 (LINC01087) has been concerned as an oncogene in breast cancer, while its mechanism in glioma has been little surveyed. Thus, we searched the prognostic value and functional action of LINC01087 in glioma. Glioma patients after preoperative MRI diagnosis were enrolled, and LINC01087, microRNA (miR)-1277-5p, and alkaline ceramidase 3 (ACER3) levels were tested in glioma cancer tissue. The correlation between LINC01087 expression and the survival of patients were analyzed. LINC01087, miR-1277-5p, and ACER3 levels in U251 cells were altered via transfection, and cell malignant phenotypes were monitored. The relationship between miR-1277-5p and LINC01087 or ACER3 was detected. The LINC01087 and ACER3 expression was in up-regulation and the miR-1277-5p expression was in down-regulation in clinical glioma samples. High expression of LINC01087 was associated with poor prognosis of glioma patients with preoperative MRI. LINC01087 silencing restrained tumor malignancy in glioma cells. Mechanistically, LINC01087 directly interacted with miR-1277-5p. ACER3 was a known target of miR-1277-5p. Moreover, rescue assays reveal that miR-1277-5p overexpression (or ACER3 overexpression) reversed the effects of LINC01087 upregulation (or miR-1277-5p upregulation) on glioma cells. LINC01087 has prognostic significance in glioma and silencing LINC01087 deters glioma development through elevating miR-1277-5p to reduce ACER3 expression.
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http://dx.doi.org/10.1007/s10142-021-00812-wDOI Listing
November 2021

A visualized ratiometric fluorescence sensing system for copper ions based on gold nanoclusters/perovskite quantum [email protected] nanocomposites.

Analyst 2021 Nov 23. Epub 2021 Nov 23.

MOE Key Laboratory of Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and College of Chemistry, Fuzhou University, Fuzhou, 350108, P.R. China.

Excessive copper ions (Cu) cause serious environmental pollution and even endanger the health of organisms. Fluorescence chemosensing materials are widely used in the detection of metal ions due to their simple operation and high sensitivity. In this study, SiO-encapsulated single perovskite quantum dot ([email protected]) core-shell nanostructures which show strong, stable, and green fluorescence are synthesized and composited with gold nanoclusters (AuNCs) which show Cu-sensitive and red light-emitting fluorescence to obtain a visualized ratiometric fluorescence sensor (AuNCs/[email protected]) for the detection of Cu. In the visualized detection of Cu, the green fluorescence emitted from the ion-insensitive [email protected] component is used as a reference signal and the red fluorescence emitted by ion-sensitive AuNC component is adopted as a sensing signal. In the presence of Cu, the red fluorescence is quenched whereas the green fluorescence remains stable, which results in a visualized fluorescence color change from orange-red to yellow and finally to green with increasing Cu concentration. The significant change in the fluorescence color of AuNCs/[email protected] in response to Cu enables a rapid, sensitive, and visualized detection of Cu. Further accurate and sensitive ratiometric fluorescence analysis of Cu can be accomplished by measuring the ratio of fluorescence intensities at 643 and 520 nm (/) at a certain Cu level. The developed AuNCs/[email protected] sensor has been validated by its satisfactory application in the detection of Cu in human serum and environmental water samples.
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http://dx.doi.org/10.1039/d1an01857dDOI Listing
November 2021

Cold and heterogeneous T cell repertoire is associated with copy number aberrations and loss of immune genes in small-cell lung cancer.

Nat Commun 2021 11 17;12(1):6655. Epub 2021 Nov 17.

Department of Thoracic/Head and Neck Medical Oncology, the University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, USA.

Small-cell lung cancer (SCLC) is speculated to harbor complex genomic intratumor heterogeneity (ITH) associated with high recurrence rate and suboptimal response to immunotherapy. Here, using multi-region whole exome/T cell receptor (TCR) sequencing as well as immunohistochemistry, we reveal a rather homogeneous mutational landscape but extremely cold and heterogeneous TCR repertoire in limited-stage SCLC tumors (LS-SCLCs). Compared to localized non-small cell lung cancers, LS-SCLCs have similar predicted neoantigen burden and genomic ITH, but significantly colder and more heterogeneous TCR repertoire associated with higher chromosomal copy number aberration (CNA) burden. Furthermore, copy number loss of IFN-γ pathway genes is frequently observed and positively correlates with CNA burden. Higher mutational burden, higher T cell infiltration and positive PD-L1 expression are associated with longer overall survival (OS), while higher CNA burden is associated with shorter OS in patients with LS-SCLC.
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http://dx.doi.org/10.1038/s41467-021-26821-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599854PMC
November 2021

Abscisic acid is required for root elongation associated with Ca influx in response to water stress.

Plant Physiol Biochem 2021 Nov 10;169:127-137. Epub 2021 Nov 10.

Center for Plant Water-Use and Nutrition Regulation and College of Resources and Environment, Joint International Research Laboratory of Water and Nutrient in Crops and College of Life Sciences, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, 350002, China. Electronic address:

Abscisic acid (ABA) is a critical hormone for plant survival upon water stress. In this study, a large-scale mutants of Arabidopsis ecotype Columbia-0 (Col-0) by ethyl methanesulfonate (EMS)-mutagenesis were generated, and an improved root elongation under water-stress 1 (irew1) mutant showing significantly enhanced root growth was isolated upon a water potential gradient assay. Then, irew1 and ABA-related mutants in Arabidopsis or tomato plants were observed under water potential gradient assay or water-deficient condition. ABA pathway, Ca response and primary root (PR) elongation rate were monitored in addition to DNA- and RNA-Seq analyses. We found that based on phenotyping and transcriptional analyses, irew1 exhibited the enhanced PR growth, ABA and Ca responses compared to wild-type subjected to water stress. Interestingly, exogenous Ca application enhanced PR growth of irew1, ABA-biosynthesis deficient mutants in Arabidopsis and tomato plants in response to water potential gradients or water-deficient condition. In combination with other ABA-related mutants and pharmacological study, our results suggest that ABA is required for root elongation associated with Ca influx in response to water stress.
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http://dx.doi.org/10.1016/j.plaphy.2021.11.002DOI Listing
November 2021

Efficient Tandem Quantum-Dot LEDs Enabled by An Inorganic Semiconductor-Metal-Dielectric Interconnecting Layer Stack.

Adv Mater 2021 Nov 10:e2108150. Epub 2021 Nov 10.

Key Laboratory of Advanced Display and System Applications of Ministry of Education, Shanghai University, 149 Yanchang Road, Shanghai, 200072, China.

Light-emitting diodes (LEDs) in a tandem configuration offer a strategy to realize high-performance, multicolor devices. Until now, though, the efficiency of tandem colloidal quantum dot LEDs (QLEDs) has been limited due to unpassivated interfaces and solvent damage originating from the materials processing requirements of interconnecting layers (ICLs). Here an ICL is reported consisting of a semiconductor-metal-dielectric stack that provides facile fabrication, materials stability, and good optoelectronic coupling. It is investigated experimentally how the ICL enables charge balance, suppresses current leakage, and prevents solvent damage to the underlying layers. As a result record efficiencies are reported for double-junction tandem QLEDs, whose emission wavelengths cover from blue to red light; i.e., external quantum efficiencies (EQEs) of 40% (average 37+/-2%) for red, 49% (average 45+/-2%) for yellow, 50% (average 46+/-2%) for green, and 24% (average 21+/-2%) for blue are achieved.
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http://dx.doi.org/10.1002/adma.202108150DOI Listing
November 2021

Relatively low tooth replacement rate in a sauropod dinosaur from the Early Cretaceous Ruyang Basin of central China.

PeerJ 2021 27;9:e12361. Epub 2021 Oct 27.

Institute of Geology, Chinese Academy of Geological Sciences, Beijing, China.

Tooth replacement rate is an important feature related to feeding mechanics and food choices for dinosaurs. However, only a few data points are available for sauropod dinosaurs, partially due to rarity of relevant fossil material. Four somphospondylan sauropod species have been recovered from the Lower Cretaceous Aptian-Albian Haoling Formation in the Ruyang Basin, Henan Province of central China, but no cranial material has been reported except for a single crown. Here we report the discovery of the rostral portion of a left dentary with replacement teeth in its first five alveoli. Comparative anatomical study shows the partial dentary can be assigned to a member of early diverging somphospondylans. The non-destructive tooth length-based approach to estimating tooth formation time and replacement rate is adopted here. The estimated tooth replacement rate is 76 days, faster than that of (83 days) and much lower than typical late diverging lithostrotian titanosaurians (20 days). Thus, this discovery adds an intermediate tooth replacement rate in the evolution of titanosauriform sauropods and supports the idea that evolution of tooth replacement rate is clade-specific. This discovery also provides more information to understand the Ruyang sauropod assemblage, which includes one of the most giant dinosaurs to have walked our Earth ().
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http://dx.doi.org/10.7717/peerj.12361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556709PMC
October 2021

Study of MOF incorporated dual layer membrane with enhanced removal of ammonia and per-/poly-fluoroalkyl substances (PFAS) in landfill leachate treatment.

Sci Total Environ 2022 Feb 30;806(Pt 4):151207. Epub 2021 Oct 30.

CSIRO Manufacturing, Private Bag 10, Clayton South, Vic. 3169, Australia.

Landfill leachate is a highly polluted and complex wastewater as it contains large amounts of organic matters, ammonia‑nitrogen, heavy metals, and per-/poly-fluoroalkyl substances (PFAS), which makes its treatment very challenging. In this paper, hydrophilic/hydrophobic dual layer membranes combining advantages of pervaporation and membrane distillation was employed to treat leachate in a direct contact membrane distillation (DCMD) configuration. An aluminum fumarate (AlFu) metal organic framework (MOF) incorporated poly(vinyl alcohol) (PVA) hydrophilic layer was coated on hydrophobic PTFE membrane to overcome the low separation efficiency of PFAS and ammonia and wetting issues encountered by the conventional hydrophobic PTFE membrane used for DCMD. The rejections of dual layer membranes with different MOF loading to PFAS, ammonia, TOC and TDS were assessed based on the amount of AlFu MOF incorporated into the PVA layer. Based on the conducted adsorption tests, it was found that AlFu MOF increases the rejection of PVA layer to PFAS and ammonia. The coating of the hydrophilic layer could enhance the wetting resistance with/without MOF addition. In comparison with the pristine PTFE membrane using synthetic feed containing 3 wt% NaCl, 1 wt% addition of AlFu MOF into the PVA layer showed slightly increased flux. All the tested membranes showed more than 99% rejection to TOC. The rejection to ammonia was increased as more MOF was incorporated into the PVA layer. The maximum rejection of ammonia was 99.8% when the PVA layer containing 10% MOF. All the membranes showed more than 99% rejection to PFOS and PFHxS. However, PTFE membrane did not show any rejection to PFOA. As more MOF was added into the hydrophilic layer, the rejection to PFOA increased, but plateaued at 65.6% with 5% MOF incorporation into the hydrophilic layer.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151207DOI Listing
February 2022

Identification of Benign and Malignant Lung Nodules in CT Images Based on Ensemble Learning Method.

Interdiscip Sci 2021 Nov 2. Epub 2021 Nov 2.

Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, 450003, Henan, China.

Background And Objective: Under the background of urgent need for computer-aided technology to provide physicians with objective decision support, aiming at reducing the false positive rate of nodule CT detection in pulmonary nodules detection and improving the accuracy of lung nodule recognition, this paper puts forward a method based on ensemble learning to distinguish between malignant and benign pulmonary nodules.

Methods: Firstly, trained on a public data set, a multi-layer feature fusion YOLOv3 network is used to detect lung nodules. Secondly, a CNN was trained to differentiate benign from malignant pulmonary nodules. Then, based on the idea of ensemble learning, the confidence probability of the above two models and the label of the training set are taken as data features to build a Logistic regression model. Finally, two test sets (public data set and private data set) were tested, and the confidence probability output by the two models was fused into the established logistic regression model to determine benign and malignant pulmonary nodules.

Results: The YOLOv3 network was trained to detect chest CT images of the test set. The number of pulmonary nodules detected in the public and private test sets was 356 and 314, respectively. The accuracy, sensitivity and specificity of the two test sets were 80.97%, 81.63%, 78.75% and 79.69%, 86.59%, 72.16%, respectively. With CNN training pulmonary nodules benign and malignant discriminant model analysis of two kinds of test set, the result of accuracy, sensitivity and specificity were 90.12%, 90.66%, 89.47% and 88.57%, 85.62%, 90.87%, respectively. Fused model based on YOLOv3 network and CNN is tested on two test sets, and the result of accuracy, sensitivity and specificity were 93.82%, 94.85%, 92.59% and 92.31%, 92.68%, 91.89%, respectively.

Conclusion: The ensemble learning model is more effective than YOLOv3 network and CNN in removing false positives, and the accuracy of the ensemble. Learning model is higher than the other two networks in identifying pulmonary nodules.
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http://dx.doi.org/10.1007/s12539-021-00472-1DOI Listing
November 2021

Mouse-INtraDuctal (MIND): an in vivo model for studying the underlying mechanisms of DCIS malignancy.

J Pathol 2021 Oct 29. Epub 2021 Oct 29.

Department of Biostatistics, The University of Kansas Medical Center, Kansas City, KS, USA.

Due to widespread adoption of screening mammography, there has been a significant increase in new diagnoses of ductal carcinoma in situ (DCIS). However, DCIS prognosis remains unclear. To address this gap, we developed an in vivo model, Mouse-INtraDuctal (MIND), in which patient-derived DCIS epithelial cells are injected intraductally and allowed to progress naturally in mice. Similarly to human DCIS, the cancer cells formed in situ lesions inside the mouse mammary ducts and mimicked all histologic subtypes including micropapillary, papillary, cribriform, solid, and comedo. Among 37 patient samples injected into 202 xenografts, at median duration of 9 months, 20 samples (54%) injected into 95 xenografts showed in vivo invasive progression while 17 (46%) samples injected into 107 xenografts remained noninvasive. Among the 20 samples that showed invasive progression, 9 samples injected into 54 xenografts exhibited a mixed pattern in which some xenografts showed invasive progression while others remained noninvasive. Among the clinically relevant biomarkers, only elevated progesterone receptor expression in patient DCIS and the extent of in vivo growth in xenografts predicted an invasive outcome. The Tempus XT assay was used on 16 patient DCIS FFPE sections including 8 DCIS that showed invasive progression, 5 DCIS that remained non-invasive and 3 DCIS that showed a mixed pattern in the xenografts. Analysis of the frequency of cancer related pathogenic mutations among the groups showed no significant differences (KW: P >0.05). There were also no differences in the frequency of high, moderate, or low severity mutations (KW; P >0.05). These results suggest that genetic changes in the DCIS are not the primary driver for the development of invasive disease. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/path.5820DOI Listing
October 2021

Enhancing Radium 223 treatment efficacy by anti-beta 1 integrin targeting.

J Nucl Med 2021 Oct 28. Epub 2021 Oct 28.

The University of Texas MD Anderson Cancer Center, United States.

Radium 223 (Ra) is an α-emitter approved for the treatment of bone metastatic prostate cancer (PCa), which exerts direct cytotoxicity towards PCa cells near the bone interface, whereas cells positioned in the core respond poorly, due to short α-particle penetrance. β1 integrin (β1I) interference has been shown to increase radiosensitivity and significantly enhance external beam radiation efficiency. We hypothesized that targeting β1I would improve Ra outcome. We tested the effect of combining Ra and anti-β1I antibody treatment in PC3 and C4-2B PCa cell models expressing high and low β1I levels, respectively. In vivo tumor growth was evaluated through bioluminescence. Cellular and molecular determinants of response were analyzed by ex vivo three-dimensional imaging of bone lesions, proteomic analysis and further confirmed by computational modeling and in vitro functional analysis in tissue-engineered bone mimetic systems. Interference with β1I combined with Ra reduced PC3 cell growth in bone and significantly improved overall mouse survival, while no change was achieved in C4-2B tumors. Anti-β1I treatment decreased PC3 tumor cell mitosis index and spatially expanded Ra lethal effects two-fold, in vivo and in silico. Regression was paralleled by decreased expression of radio-resistance mediators. Targeting β1I significantly improves Ra outcome and points towards combinatorial application in PCa tumors with high β1I expression.
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http://dx.doi.org/10.2967/jnumed.121.262743DOI Listing
October 2021

Flexible core/shelled [email protected] nanotube porous films for hybrid supercapacitors.

Nanotechnology 2021 Nov 18;33(6). Epub 2021 Nov 18.

Xi'an Key Laboratory of New Energy Materials and Devices, School of Materials Science and Engineering, Xi'an University of Technology, Xi'an, Shaanxi, 710048, People's Republic of China.

Flexibility of the films and the limited ion transport in the vertical direction of film highly restrict the development of flexible supercapacitors. Herein, we have developed hybrid porous films consisting of N-doped holey graphene nanosheets (NHGR) with abundant in-plane nanopores and the vertically aligned polyaniline nanowires arrays on polypyrrole nanotubes ([email protected]) via a two-step oxidative polymerization strategy and vacuum filtration. The rational design can efficiently shorten the diffusion path of electrons/ions, alleviate volume variation of electrodes during cycling, enhance electric conductivity of the hybrids, and while offer abundant active interfacial sites for electrochemical reaction. Benefiting from the distinctive structural and compositional merits, the obtained [email protected]/NHGR film electrode manifests an excellent electrochemical properties in terms of specific capacity (1348 mF cmat a current density of 1 mA cm), rate capability (81.2% capacitance retention from 1 to 30 mA cm), and cycling stability (capacitance retention of 73.7% at 20 mA cmafter 7000 cycles). Matched with NHGR negative electrode, the assembled flexible all-solid-state asymmetric supercapacitor displays a remarkable areal capacitance of 359 mF cmat 5 mA cm, maximum areal energy density of 112.2Wh cmat 3.747 mW cm, and good flexibility at various bending angles while preserving stable cycling performance. The result shows the [email protected]/NHGR film with high flexibility and 3D ions transport channels is highly attractive for flexible energy storage devices.
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http://dx.doi.org/10.1088/1361-6528/ac3359DOI Listing
November 2021

Low-Temperature Fabrication of IZO Thin Film for Flexible Transistors.

Nanomaterials (Basel) 2021 Sep 29;11(10). Epub 2021 Sep 29.

Key Laboratory of Advanced Display and System Application, Ministry of Education, Shanghai University, Shanghai 200072, China.

Solution-processed thin film transistors (TFTs) used in flexible electronics require them to be fabricated under low temperature. Ultraviolet (UV) treatment is an effective method to transform the solution precursors into dense semiconductor films. In our work, high-quality indium zinc oxide (IZO) thin films were prepared from nitrate-based precursors after UV treatment at room temperature. After UV treatment, the structure of IZO thin films was gradually rearranged, resulting in good M-O-M network formation and bonds. TFTs using IZO as a channel layer were also fabricated on Si and Polyimide (PI) substrate. The field effect mobility, threshold voltage (), and subthreshold swing () for rigid and flexible IZO TFTs are 14.3 and 9.5 cm/Vs, 1.1 and 1.7 V, and 0.13 and 0.15 V/dec., respectively. This low-temperature processed route will definitely contribute to flexible electronics fabrication.
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http://dx.doi.org/10.3390/nano11102552DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539277PMC
September 2021

Metabolic derangement in polycystic kidney disease mouse models is ameliorated by mitochondrial-targeted antioxidants.

Commun Biol 2021 10 20;4(1):1200. Epub 2021 Oct 20.

Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.

Autosomal dominant polycystic kidney disease (ADPKD) is characterized by progressively enlarging cysts. Here we elucidate the interplay between oxidative stress, mitochondrial dysfunction, and metabolic derangement using two mouse models of PKD1 mutation, PKD1 and PKD1. Mouse kidneys with PKD1 mutation have decreased mitochondrial complexes activity. Targeted proteomics analysis shows a significant decrease in proteins involved in the TCA cycle, fatty acid oxidation (FAO), respiratory complexes, and endogenous antioxidants. Overexpressing mitochondrial-targeted catalase (mCAT) using adeno-associated virus reduces mitochondrial ROS, oxidative damage, ameliorates the progression of PKD and partially restores expression of proteins involved in FAO and the TCA cycle. In human ADPKD cells, inducing mitochondrial ROS increased ERK1/2 phosphorylation and decreased AMPK phosphorylation, whereas the converse was observed with increased scavenging of ROS in the mitochondria. Treatment with the mitochondrial protective peptide, SS31, recapitulates the beneficial effects of mCAT, supporting its potential application as a novel therapeutic for ADPKD.
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http://dx.doi.org/10.1038/s42003-021-02730-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528863PMC
October 2021

SOAT1 enhances lung cancer invasion through stimulating AKT-mediated mitochondrial fragmentation.

Biochem Cell Biol 2021 Oct 20. Epub 2021 Oct 20.

Southern Medical University, 70570, Department of Respiratory and Critical Care Medicine, Guangzhou, China;

Sterol O-acyltransferase 1 (SOAT1) is a key enzyme in lipid metabolism, which mediates cholesterol esterification metabolism and is closely associated with many cancers. However, the role of SOAT1 in lung cancer invasion remains unclear. We found that SOAT1 expression was positively correlated with lung cancer invasion. Downregulation of SOAT1 inhibited invasion, mitochondrial fragmentation, AKT phosphorylation, and phospho-Drp (Ser616) in lung cancer cells and promoted intracellular free cholesterol accumulation. Mechanistically, AKT phosphorylation inhibitor MK-2206 alleviated both SOAT1 overexpression or high expression-induced mitochondrial fragmentation and lung cancer cell invasion. Furthermore, intracellular free cholesterol accumulation reduced AKT phosphorylation, SREBP1 mRNA expression, cell invasion, and mitochondrial fragmentation in lung cancer cells with high SOAT1 expression. In summary, our findings suggest that SOAT1 promotes lung cancer invasion activates the PI3K/AKT signaling pathway by downregulating intracellular free cholesterol levels, thereby affecting the regulation of mitochondrial fragmentation.
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http://dx.doi.org/10.1139/bcb-2021-0175DOI Listing
October 2021

Fasting drives the metabolic, molecular and geroprotective effects of a calorie-restricted diet in mice.

Nat Metab 2021 10 18;3(10):1327-1341. Epub 2021 Oct 18.

Department of Medicine, University of Wisconsin-Madison, Madison, WI, USA.

Calorie restriction (CR) promotes healthy ageing in diverse species. Recently, it has been shown that fasting for a portion of each day has metabolic benefits and promotes lifespan. These findings complicate the interpretation of rodent CR studies, in which animals typically eat only once per day and rapidly consume their food, which collaterally imposes fasting. Here we show that a prolonged fast is necessary for key metabolic, molecular and geroprotective effects of a CR diet. Using a series of feeding regimens, we dissect the effects of calories and fasting, and proceed to demonstrate that fasting alone recapitulates many of the physiological and molecular effects of CR. Our results shed new light on how both when and how much we eat regulate metabolic health and longevity, and demonstrate that daily prolonged fasting, and not solely reduced caloric intake, is likely responsible for the metabolic and geroprotective benefits of a CR diet.
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http://dx.doi.org/10.1038/s42255-021-00466-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8544824PMC
October 2021

Single cell T cell landscape and T cell receptor repertoire profiling of AML in context of PD-1 blockade therapy.

Nat Commun 2021 10 18;12(1):6071. Epub 2021 Oct 18.

Department of Leukemia, University of Texas MD Anderson Cancer Center, Houston, TX, USA.

In contrast to the curative effect of allogenic stem cell transplantation in acute myeloid leukemia via T cell activity, only modest responses are achieved with checkpoint-blockade therapy, which might be explained by T cell phenotypes and T cell receptor (TCR) repertoires. Here, we show by paired single-cell RNA analysis and TCR repertoire profiling of bone marrow cells in relapsed/refractory acute myeloid leukemia patients pre/post azacytidine+nivolumab treatment that the disease-related T cell subsets are highly heterogeneous, and their abundance changes following PD-1 blockade-based treatment. TCR repertoires expand and primarily emerge from CD8 cells in patients responding to treatment or having a stable disease, while TCR repertoires contract in therapyresistant patients. Trajectory analysis reveals a continuum of CD8 T cell phenotypes, characterized by differential expression of granzyme B and a bone marrow-residing memory CD8 T cell subset, in which a population with stem-like properties expressing granzyme K is enriched in responders. Chromosome 7/7q loss, on the other hand, is a cancer-intrinsic genomic marker of PD-1 blockade resistance in AML. In summary, our study reveals that adaptive T cell plasticity and genomic alterations determine responses to PD-1 blockade in acute myeloid leukemia.
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http://dx.doi.org/10.1038/s41467-021-26282-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524723PMC
October 2021

Epsilon-poly-L-lysine: Recent Advances in Biomanufacturing and Applications.

Front Bioeng Biotechnol 2021 28;9:748976. Epub 2021 Sep 28.

The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.

ε-poly-L-lysine (ε-PL) is a naturally occurring poly(amino acid) of varying polymerization degree, which possesses excellent antimicrobial activity and has been widely used in food and pharmaceutical industries. To provide new perspectives from recent advances, this review compares several conventional and advanced strategies for the discovery of wild strains and development of high-producing strains, including isolation and culture-based traditional methods as well as genome mining and directed evolution. We also summarize process engineering approaches for improving production, including optimization of environmental conditions and utilization of industrial waste. Then, efficient downstream purification methods are described, including their drawbacks, followed by the brief introductions of proposed antimicrobial mechanisms of ε-PL and its recent applications. Finally, we discuss persistent challenges and future perspectives for the commercialization of ε-PL.
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http://dx.doi.org/10.3389/fbioe.2021.748976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506220PMC
September 2021

Autoantibody of interleukin-17A induced by recombinant attenuates airway inflammation in mice with neutrophilic asthma.

J Asthma 2021 Nov 18:1-10. Epub 2021 Nov 18.

Department of Pediatrics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Objective: Previous studies have shown Interleukin (IL)-17A as an important contributor to the development of severe asthma, which is mainly characterized by neutrophilic inflammation and less response to corticosteroids. Consequently, the IL-17A-neutrophil axis could be a potential therapeutic target. Previously, we constructed a recombinant (rMS) expressing fusion protein Ag85A-IL-17A, and confirmed it could induce production of IL-17A autoantibody . This study uses a murine model of neutrophilic asthma to further investigate the effects of rMS on airway inflammation.

Methods: DO11.10 mice were divided into four groups: phosphate buffered saline (PBS), asthma, rMS and MS. This murine model of neutrophilic asthma was established with ovalbumin (OVA) challenge, whereby PBS, rMS and MS were administered intranasally. Anti-inflammatory effects on inflammatory cell infiltration and expression of inflammatory mediators in bronchoalveolar lavage fluid (BALF) were evaluated, along with histopathological changes in lung tissues.

Results: A sustained high-titer IL-17A autoantibody was detected in sera of the rMS group. Compared to the asthma group, the number of neutrophils, IL-17A, CXCL-1 levels and MPO activity in the rMS group were all significantly reduced ( < 0.01). Histological analysis showed rMS remarkably suppressed inflammatory infiltration around bronchia. The inflammation score and the mucus score in the rMS group were both significantly lower than those in the asthma group ( < 0.001).

Conclusion: rMS ameliorated airway inflammation in mice with neutrophilic asthma caused by inducing IL-17A autoantibody and regulating the IL-17A-neutrophil axis, thus offering a possible novel treatment for neutrophilic asthma.
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http://dx.doi.org/10.1080/02770903.2021.1989696DOI Listing
November 2021

Gut Microbiome Features Associated with Liver Fibrosis in Hispanics, a Population at High Risk for Fatty Liver Disease.

Hepatology 2021 Oct 11. Epub 2021 Oct 11.

Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.

Background And Aims: Hispanics are disproportionately affected by non-alcoholic fatty liver disease, liver fibrosis, cirrhosis, and hepatocellular carcinoma. Preventive strategies and non-invasive means to identify those in this population at high risk for liver fibrosis, are urgently needed. We aimed to characterize the gut microbiome signatures and related biological functions associated with liver fibrosis in Hispanics and identify environmental and genetic factors affecting them.

Approach And Results: Subjects of the population-based Cameron County Hispanic Cohort (n=217) were screened by vibration-controlled transient elastography (FibroScan). Among them, 144 (66.7%) had steatosis and 28 (13.0%) had liver fibrosis. The gut microbiome of subjects with liver fibrosis was enriched with immunogenic commensals (e.g. Prevotella copri, Holdemanella, Clostridiaceae 1) and depleted of Bacteroides caccae, Parabacteroides distasonis, Enterobacter and Marinifilaceae. The liver fibrosis-associated metagenome was characterized by changes in the urea cycle, L-citrulline biosynthesis and creatinine degradation pathways, and altered synthesis of B vitamins and lipoic acid. These metagenomic changes strongly correlated with the depletion of Parabacteroides distasonis and enrichment of Prevotella and Holdemanella. Liver fibrosis was also associated with depletion of bacterial pathways related to L-fucose biosynthesis. Alcohol consumption, even moderate, was associated with high Prevotella abundance. The single nucleotide polymorphisms rs3769502 and rs7573751 in the NCK2 gene positively associated with high Prevotella abundance.

Conclusion: Hispanics with liver fibrosis display microbiome profiles and associated functional changes that may promote oxidative stress and a pro-inflammatory environment. These microbiome signatures, together with NCK2 polymorphisms, may have utility in risk modeling and disease prevention in this high-risk population.
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http://dx.doi.org/10.1002/hep.32197DOI Listing
October 2021

Establishment of a Lung Cancer Discriminative Model Based on an Optimized Support Vector Machine Algorithm and Study of Key Targets of Wogonin in Lung Cancer.

Front Pharmacol 2021 22;12:728937. Epub 2021 Sep 22.

Clinical Bioinformatics Experimental Center, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, China.

An optimized support vector machine model was used to construct a lung cancer diagnosis model based on serological indicators, and a molecular regulation model of Wogonin, a component of , was established. Serological indexes of patients were collected, the grid search method was used to identify the optimal penalty coefficient C and parameter g of the support vector machine model, and the benign and malignant auxiliary diagnosis model of isolated pulmonary nodules based on serological indicators was established. The regulatory network and key targets of Wogonin in lung cancer were analyzed by network pharmacology, and key targets were detected by western blot. The relationship between serological susceptibility genes and key targets of Wogonin was established, and the signaling pathway of Wogonin regulating lung cancer was constructed. After support vector machine parameter optimization ( = 90.597, = 32), the accuracy of the model was 90.8333%, with nine false positives and two false negative cases. Ontology functional analysis of 67 common genes between Wogonin targets and lung cancer-related genes showed that the targets were associated with biological processes involved in peptidye-serine modification and regulation of protein kinase B signaling; cell components in the membrane raft and chromosomal region; and molecular function in protein serine/threonine kinase activity and heme binding. Kyoto Encyclopedia of Genes and Genomes analysis showed that the regulation pathways involved the PI3K-Akt signaling pathway, ERBB signaling pathway, and EGFR tyrosine kinase inhibitor resistance. analyses using lung cancer cells showed that Wogonin led to significantly increased levels of cleaved caspase-3 and Bad and significantly decreased Bcl-2 expression in a concentration-dependent manner. ErbB4 expression also significantly decreased in lung cancer cells after treatment with Wogonin. A regulatory network of Wogonin regulating lung cancer cell apoptosis was constructed, including the participation of serological susceptibility genes. There is a certain regulatory effect between the serological indexes that can be used in the diagnosis of lung cancer and the key targets of Chinese herbal medicine treatment of lung cancer, which provides a new idea for the diagnosis, treatment and prognosis of clinical lung cancer.
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http://dx.doi.org/10.3389/fphar.2021.728937DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8493220PMC
September 2021

Derivation and characterization of two human induced pluripotent stem cell lines (NUIGi004-A) and (NUIGi012-A) from two patients with LQT2 disease.

Stem Cell Res 2021 10 3;56:102555. Epub 2021 Oct 3.

Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI), Galway, Ireland; National Children's Research Centre, Children's Health Ireland at Crumlin, Dublin 12, Ireland.

Long QT syndrome type 2 (LQT2) is associated with KCNH2, which encodes the α subunit of the ion channel that controls the K current in the heart. Mutations of KCNH2 cause loss of Kv11.1 channel function by disrupting subunit folding, assembly, or trafficking of the channel to the cell surface. Here we generated two induced pluripotent stem cell (iPSC) lines from two patients carrying mutation in KCNH2 gene. These iPSCs express the pluripotent markers and have the capacity of differentiation into other cell types. These patient-derived iPSCs are useful for investigating the disease pathology and identifying the therapeutic target.
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http://dx.doi.org/10.1016/j.scr.2021.102555DOI Listing
October 2021

Coordination of root auxin with the fungus Piriformospora indica and bacterium Bacillus cereus enhances rice rhizosheath formation under soil drying.

ISME J 2021 Oct 7. Epub 2021 Oct 7.

Center for Plant Water-use and Nutrition Regulation and College of Life Sciences, Joint International Research Laboratory of Water and Nutrient in Crop, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Moderate soil drying (MSD) is a promising agricultural technique that can reduce water consumption and enhance rhizosheath formation promoting drought resistance in plants. The endophytic fungus Piriformospora indica (P. indica) with high auxin production may be beneficial for rhizosheath formation. However, the integrated role of P. indica with native soil microbiome in rhizosheath formation is unclear. Here, we investigated the roles of P. indica and native bacteria on rice rhizosheath formation under MSD using high-throughput sequencing and rice mutants. Under MSD, rice rhizosheath formation was significantly increased by around 30% with P. indica inoculation. Auxins in rice roots and P. indica were responsible for the rhizosheath formation under MSD. Next, the abundance of the genus Bacillus, known as plant growth-promoting rhizobacteria, was enriched in the rice rhizosheath and root endosphere with P. indica inoculation under MSD. Moreover, the abundance of Bacillus cereus (B. cereus) with high auxin production was further increased by P. indica inoculation. After inoculation with both P. indica and B. cereus, rhizosheath formation in wild-type or auxin efflux carrier OsPIN2 complemented line rice was higher than that of the ospin2 mutant. Together, our results suggest that the interaction of the endophytic fungus P. indica with the native soil bacterium B. cereus favors rice rhizosheath formation by auxins modulation in rice and microbes under MSD. This finding reveals a cooperative contribution of P. indica and native microbiota in rice rhizosheath formation under moderate soil drying, which is important for improving water use in agriculture.
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http://dx.doi.org/10.1038/s41396-021-01133-3DOI Listing
October 2021

Corrigendum to 'Three-dimensional printed strontium-containing mesoporous bioactive glass scaffolds for repairing rat critical-sized calvarial defects' [Acta Biomaterialia 12 (2015) 270-280].

Acta Biomater 2021 Oct 4;134:819-820. Epub 2021 Oct 4.

Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, 600 Yishan Road, Shanghai 200233, People's Republic of China. Electronic address:

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http://dx.doi.org/10.1016/j.actbio.2021.09.044DOI Listing
October 2021

Identification of ABC transporter G subfamily in white lupin and functional characterization of L.albABGC29 in phosphorus use.

BMC Genomics 2021 Oct 6;22(1):723. Epub 2021 Oct 6.

Joint International Research Laboratory of Water and Nutrient in Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Background: White lupin (Lupinus albus) is a leguminous crop with elite adaptive ability in phosphorus-deficient soil and used as a model plant for studying phosphorus (P) use. However, the genetic basis of its adaptation to low P (LP) remains unclear. ATPase binding cassette (ABC) transports G subfamily play a crucial role in the transportation of biological molecules across the membrane. To date, identification of this subfamily has been analyzed in some plants, but no systematic analysis of these transporters in phosphorus acquisition is available for white lupin.

Results: This study identified 66 ABCG gene family members in the white lupin genome using comprehensive approaches. Phylogenetic analysis of white lupin ABCG transporters revealed six subclades based on their counterparts in Arabidopsis, displaying distinct gene structure and motif distribution in each cluster. Influences of the whole genome duplication on the evolution of L.albABCGs were investigated in detail. Segmental duplications appear to be the major driving force for the expansion of ABCGs in white lupin. Analysis of the Ka/Ks ratios indicated that the paralogs of the L.albABCG subfamily members principally underwent purifying selection. However, it was found that L.albABCG29 was a result of both tandem and segmental duplications. Overexpression of L.albABCG29 in white lupin hairy root enhanced P accumulation in cluster root under LP and improved plant growth. Histochemical GUS staining indicated that L.albABCG29 expression increased under LP in white lupin roots. Further, overexpression of L.albABCG29 in rice significantly improved P use under combined soil drying and LP by improving root growth associated with increased rhizosheath formation.

Conclusion: Through systematic and comprehensive genome-wide bioinformatics analysis, including conserved domain, gene structures, chromosomal distribution, phylogenetic relationships, and gene duplication analysis, the L.albABCG subfamily was identified in white lupin, and L.albABCG29 characterized in detail. In summary, our results provide deep insight into the characterization of the L.albABCG subfamily and the role of L.albABCG29 in improving P use.
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http://dx.doi.org/10.1186/s12864-021-08015-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495970PMC
October 2021

Therapeutic Nanoparticles from Grape Seed for Modulating Oxidative Stress.

Small 2021 Nov 3;17(45):e2102485. Epub 2021 Oct 3.

College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, China.

The therapeutic potential of nanomaterials toward oxidative damage relevant diseases has attracted great attentions by offering promising advantages compared with conventional antioxidants. Although different kinds of nanoantioxidants have been well developed, the facile fabrication of robust and efficient nanoscavengers is still met with challenges like the use of toxic and high-cost subunits, the involvement of multistep synthetic process, and redundant purification work. Herein, a direct fabrication strategy toward polyphenol nanoparticles with tunable size, excellent biocompatibility, and reactive oxygen species (ROS) scavenging capacities from grape seed via an enzymatic polymerization method is reported. The resulting nanoparticles can efficiently prevent cell damage from ROS and exert promising in vivo antioxidant therapeutic effects on several oxidative stress-related diseases, including accelerating wound healing, inhibiting ulcerative colitis, and regulating the oxidative stress in dry eye disease. This study can stimulate the development of more kinds of low-cost, safe, and efficient biomass-based antioxidative nanomaterials via similar fabrication methodologies.
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http://dx.doi.org/10.1002/smll.202102485DOI Listing
November 2021

Modulation of STIM1 by a risk insertion/deletion polymorphism underlying genetics susceptibility to sudden cardiac death originated from coronary artery disease.

Forensic Sci Int 2021 Nov 22;328:111010. Epub 2021 Sep 22.

Department of Forensic Medicine, Medical College of Soochow University, Suzhou, China. Electronic address:

Stromal interaction molecule 1 (STIM1), as a dynamic calcium signal transducer and key regulator of cardiomyocyte Ca homeostasis, has been implicated in various pathological processes related to sudden cardiac death originated from coronary artery disease (SCD-CAD). In this study, we performed a systematic variant screening on promoter region of STIM1 to filter potential functional genetic variations. Based on the screening results, a 5-bp insertion/deletion (indel) polymorphism (rs3061890) in promoter region of STIM1 was selected as the candidate variant. We investigated the association of rs3061890 with SCD-CAD susceptibility in Chinese Han populations. The homozygote del/del genotype significantly increased risk for SCD-CAD as compared with the ins/ins genotype (odds ratio, 2.86 [95% confidence interval, 1.69-4.29]; P = 2.3 × 10-5). Compared with the common allele, the 5-bp deletion risk allele exhibited lower transcriptional capacity in luciferase assays. Intriguingly, genotype-phenotype correlation studies using human myocardium tissue samples revealed that the expression of STIM1 was associated with the genotype of rs3061890. Computational prediction combined with electrophoretic mobility shift (EMSA) and chromatin immunoprecipitation (ChIP) assays provided convincing evidence for stronger binding affinity of ELF1 (E74 like ETS transcription factor 1) with the deletion allele promoter. Taken together, our findings implied an allele-specific mechanism of regulating the transcription of STIM1 via ELF1, which contribute to SCD-CAD susceptibility. rs3061890 may thus considered as a candidate genetic marker for SCD-CAD prediction and prevention.
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http://dx.doi.org/10.1016/j.forsciint.2021.111010DOI Listing
November 2021
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