Publications by authors named "Jianhua Wei"

74 Publications

Isoorientin Affects Markers of Alzheimer's Disease via Effects on the Oral and Gut Microbiota in APP/PS1 Mice.

J Nutr 2021 Oct 12. Epub 2021 Oct 12.

Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

Background: There is growing evidence of strong associations between the pathogenesis of Alzheimer's disease (AD) and dysbiotic oral and gut microbiota. Recent studies demonstrated that isoorientin (ISO) is anti-inflammatory and alleviates markers of AD, which were hypothesized to be mediated by the oral and gut microbiota.

Objectives: We studied the effects of oral administration of ISO on AD-related markers and the oral and gut microbiota in mice.

Methods: Eight-month-old amyloid precursor protein/presenilin-1 (AP) transgenic male mice were randomly allocated to 3 groups of 15 mice each: vehicle (AP) alone or with a low dose of ISO (AP + ISO-L; 25 mg/kg) or a high dose of ISO (AP + ISO-H; 50 mg/kg). Age-matched wild-type (WT) C57BL/6 male littermates were used as controls. The 4 groups were treated intragastrically with ISO or sterilized ultrapure water for 2 months. AD-related markers in the brain, serum, colon, and liver were analyzed with immunohistochemical and histochemical staining, Western blotting, and ELISA. Oral and gut microbiotas were analyzed using 16S ribosomal RNA gene sequencing.

Results: The high-dose ISO treatment significantly decreased amyloid beta 42-positive deposition by 38.1% and 45.2% in the cortex and hippocampus, respectively, of AP mice (P < 0.05). Compared with the AP group, both ISO treatments reduced brain phospho-Tau, phosphor-p65, phosphor-inhibitor of NF-κB, and brain and serum LPS and TNF-α by 17.9%-72.5% and increased brain and serum IL-4 and IL-10 by 130%-210% in the AP + ISO-L and AP + ISO-H groups (P < 0.05). Abundances of 26, 25, and 23 microbial taxa in oral, fecal and cecal samples, respectively, were increased in both the AP + ISO-L and AP + ISO-H groups relative to the AP group [linear discriminant analysis (LDA) >3.0; P < 0.05]. Gram-negative bacteria, Alteromonas, Campylobacterales, and uncultured Bacteroidales bacterium were positively correlated (rho = 0.28-0.59; P < 0.05) with the LPS levels and responses of inflammatory cytokines.

Conclusions: The microbiota-gut-brain axis is a potential mechanism by which ISO reduces AD-related markers in AP mice.
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http://dx.doi.org/10.1093/jn/nxab328DOI Listing
October 2021

Effectiveness of chlorhexidine in preventing infections among patients undergoing cardiac surgeries: a meta-analysis and systematic review.

Antimicrob Resist Infect Control 2021 Oct 7;10(1):140. Epub 2021 Oct 7.

Surgical Intensive Care Unit, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, China.

Background: Although several meta-analyses reported the impact of chlorhexidine (CHX) use in patients undergoing various types of surgery, no meta-analysis summarized the overall effectiveness of CHX specifically for cardiac surgery. This meta-analysis aimed to examine the impact of CHX on infections after cardiac surgery compared with other cleansers or antiseptics.

Methods: PubMed, Embase, and the Cochrane Library were searched from inception up to October 2020 for potentially eligible studies: (1) population: patients who underwent cardiac surgery; (2) intervention or exposure: any type of CHX use in the treatment or exposed group; (3) outcome: number of patients with infections; (4) comparison: placebo or other antiseptic agents; (5) English. The primary outcome was surgical site infection (SSI).

Results: Fourteen studies were included, with 8235 and 6901 patients in the CHX and control groups. CHX was not protective against SSI (OR = 0.77, 95% CI: 0.57-1.04, P = 0.090). CHX was protective for superficial wound infection (OR = 0.42, 95% CI: 0.26-0.70, P = 0.001), but not with deep wound infection (P = 0.509). CHX was not protective against urinary tract of infection (P = 0.415) but was protective for bloodstream infection (OR = 0.36, 95% CI: 0.16-0.80, P = 0.012), nosocomial infections (OR = 0.55, 95% CI: 0.44-0.69, P < 0.001), and pneumonia (OR = 0.26, 95% CI: 0.11-0.61, P = 0.002).

Conclusions: In patients undergoing cardiac surgery, CHX does not protect against SSI, deep wound infection, and urinary tract infections but might protect against superficial SSI, bloodstream infection, nosocomial infections, and pneumonia.
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http://dx.doi.org/10.1186/s13756-021-01009-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8499511PMC
October 2021

PmiREN2.0: from data annotation to functional exploration of plant microRNAs.

Nucleic Acids Res 2021 Sep 23. Epub 2021 Sep 23.

Beijing Agro-biotechnology Research Center, Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, P.R. China.

Nearly 200 plant genomes have been sequenced over the last two years, and new functions of plant microRNAs (miRNAs) have been revealed. Therefore, timely update of the plant miRNA databases by incorporating miRNAs from the newly sequenced species and functional information is required to provide useful resources for advancing plant miRNA research. Here we report the update of PmiREN2.0 (https://pmiren.com/) with an addition of 19 363 miRNA entries from 91 plants, doubling the amount of data in the original version. Meanwhile, abundant regulatory information centred on miRNAs was added, including predicted upstream transcription factors through binding motifs scanning and elaborate annotation of miRNA targets. As an example, a genome-wide regulatory network centred on miRNAs was constructed for Arabidopsis. Furthermore, phylogenetic trees of conserved miRNA families were built to expand the understanding of miRNA evolution across the plant lineages. These data are helpful to deduce the regulatory relationships concerning miRNA functions in diverse plants. Beside the new data, a suite of design tools was incorporated to facilitate experimental practice. Finally, a forum named 'PmiREN Community' was added for discussion and resource and new discovery sharing. With these upgrades, PmiREN2.0 should serve the community better and accelerate miRNA research in plants.
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http://dx.doi.org/10.1093/nar/gkab811DOI Listing
September 2021

Entrepreneurship, Corporate Social Responsibilities, and Innovation Impact on Banks' Financial Performance.

Front Psychol 2021 25;12:680661. Epub 2021 Aug 25.

College of Business Administration, King Saud University - Muzahimiyah Branch, Al-Muzahmiya, Saudi Arabia.

The basic aim of this research was to check the impact of innovation, corporate social responsibilities (CSR), and entrepreneurship on the monetary performance of banks in five different countries: Qatar, Pakistan, China, the United States (US), and France. This research was conducted to measure the relationship of these factors and innovative workforce activities. The secondary data were collected from websites of twenty five banks in different countries, including Islamic and conventional banks. Different econometric analyses, such as descriptive statistical analysis, correlation coefficient test for measuring the interaction, and ordinary least square regression analysis for determining the impact of dependent and independent variables, were carried out. In the present study, entrepreneurship, CSR, and innovation were taken as independent variables. Board size, frequency of assemblies, and self-employed with large shareholders were included as sub-parts of entrepreneurship. On the other hand, the financial performance of banks was taken as the dependent variable. Return on assets (ROA) and return on equity (ROE) were considered parts of economic performance. The overall conclusions drawn in this study showed that there was a significant relationship between all the studied variables. The research provided useful insights into the long-debated question regarding the relevance of entrepreneurship and CSR.
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http://dx.doi.org/10.3389/fpsyg.2021.680661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425480PMC
August 2021

Clinical Characteristics of Human Adenovirus Plastic Bronchitis in 10 Pediatric Cases: A Retrospective Study of Seven Years.

Virol Sin 2021 Jun 22;36(3):550-554. Epub 2021 Jun 22.

Department of Respiratory Medicine, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorder, Children's Hospital of Chongqing Medical University, Chongqing Key Laboratory of Pediatrics, Chongqing, 400014, China.

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http://dx.doi.org/10.1007/s12250-021-00394-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218560PMC
June 2021

Population-scale peach genome analyses unravel selection patterns and biochemical basis underlying fruit flavor.

Nat Commun 2021 06 14;12(1):3604. Epub 2021 Jun 14.

Beijing Agro-Biotechnology Research Center, Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing, China.

A narrow genetic basis in modern cultivars and strong linkage disequilibrium in peach (Prunus persica) has restricted resolution power for association studies in this model fruit species, thereby limiting our understanding of economically important quality traits including fruit flavor. Here, we present a high-quality genome assembly for a Chinese landrace, Longhua Shui Mi (LHSM), a representative of the Chinese Cling peaches that have been central in global peach genetic improvement. We also map the resequencing data for 564 peach accessions to this LHSM assembly at an average depth of 26.34× per accession. Population genomic analyses reveal a fascinating history of convergent selection for sweetness yet divergent selection for acidity in eastern vs. western modern cultivars. Molecular-genetics and biochemical analyses establish that PpALMT1 (aluminum-activated malate transporter 1) contributes to their difference of malate content and that increases fructose content accounts for the increased sweetness of modern peach fruits, as regulated by PpERDL16 (early response to dehydration 6-like 16). Our study illustrates the strong utility of the genomics resources for both basic and applied efforts to understand and exploit the genetic basis of fruit quality in peach.
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http://dx.doi.org/10.1038/s41467-021-23879-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203738PMC
June 2021

A novel 1,8-naphthalimide-based Cu ion fluorescent probe and its bioimaging application.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Nov 1;261:120037. Epub 2021 Jun 1.

School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou 221116, People's Republic of China. Electronic address:

A new 1,8-naphthalimide-based Schiff base compound, named as (Z)-2-butyl-6-(((2-hydroxyphenyl)imino)methyl)-1H-benzo[de]isoquinoline-1,3(2H)-dione (BHBD), has been simply synthesized with high yields. BHBD can be employed as a "turn-on" fluorescent probe for Cu ion with high sensitivity, high selectivity and relatively low detection limit (0.48 × 10 M). The fluorescence emission of BHBD is very weak in HO/THF (v/v: 7/3) mixture, which is significantly enhanced after addition of Cu ion. The proposed mechanism is verified by H NMR, Job's plot and TOF-MS experiments. Anti-interference experiment, cytotoxicity assay and pH influence results indicated that BHBD meets the requirements of bioimaging. Therefore, BHBD has been successfully applied in detecting Cu ion in HeLa cells.
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http://dx.doi.org/10.1016/j.saa.2021.120037DOI Listing
November 2021

Polyene-Producing spp. From the Fungus-Growing Termite Exhibit High Inhibitory Activity Against the Antagonistic Fungus .

Front Microbiol 2021 1;12:649962. Epub 2021 Apr 1.

State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, China.

Fungus-growing termites are engaged in a tripartite mutualism with intestinal microbes and a monocultivar ( sp.) in the fungus garden. The termites are often plagued by entomopathogen () and fungus garden is always threatened by competitors ( spp.). Here, we aim to understand the defensive role of intestinal microbes, the actinomycetes which were isolated from the gut of . We obtained 44 antifungal isolates, which showed moderate to strong inhibition to sp. HPLC analysis indicated that different types of polyenes (tetraene, pentene, and heptaene) existed in the metabolites of 10 strong antifungal strains. Two pentene macrolides (pentamycin and 1'14-dihydroxyisochainin) were firstly purified from strain HF10, both exhibiting higher activity against sp. and than cultivar . Subsequently, tetraene and heptaene related gene disruption assay showed that the mutant strains lost the ability to produce corresponding polyenes, and they also had significantly decreased activities against sp. and compared to that of wild type strains. These results indicate that polyene-producing from the guts of have strong inhibition to competitor fungus and polyenes contribute to inhibitory effects on sp.
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http://dx.doi.org/10.3389/fmicb.2021.649962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047067PMC
April 2021

Design, Synthesis and Pharmacological Evaluation of Three Novel Dehydroabietyl Piperazine Dithiocarbamate Ruthenium (II) Polypyridyl Complexes as Potential Antitumor Agents: DNA Damage, Cell Cycle Arrest and Apoptosis Induction.

Molecules 2021 Mar 7;26(5). Epub 2021 Mar 7.

School of Pharmacy, Guilin Medical University, Guilin 541199, China.

The use of cisplatin is severely limited by its toxic side-effects, which has spurred chemists to employ different strategies in the development of new metal-based anticancer agents. Here, three novel dehydroabietyl piperazine dithiocarbamate ruthenium (II) polypyridyl complexes (-) were synthesized as antitumor agents. Compounds and exhibited better in vitro antiproliferative activity against seven tumor cell lines than cisplatin, they displayed no evident resistance in the cisplatin-resistant cell line A549/DPP. Importantly, effectively inhibited tumor growth in the T-24 xenograft mouse model in comparison with cisplatin. Gel electrophoresis assay indicated that DNA was the potential targets of and , and the upregulation of p-H2AX confirmed this result. Cell cycle arrest studies demonstrated that and arrested the cell cycle at G1 phase, accompanied by the upregulation of the expression levels of the antioncogene p27 and the down-regulation of the expression levels of cyclin E. In addition, and caused the apoptosis of tumor cells along with the upregulation of the expression of Bax, caspase-9, cytochrome c, intracellular Ca release, reactive oxygen species (ROS) generation and the downregulation of Bcl-2. These mechanistic study results suggested that and exerted their antitumor activity by inducing DNA damage, and consequently causing G1 stage arrest and the induction of apoptosis.
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http://dx.doi.org/10.3390/molecules26051453DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962184PMC
March 2021

Correction to: High‑depth resequencing of 312 accessions reveals the local adaptation of foxtail millet.

Theor Appl Genet 2021 May;134(5):1603

Beijing Agro-Biotechnology Research Center, Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.

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http://dx.doi.org/10.1007/s00122-021-03817-yDOI Listing
May 2021

High-depth resequencing of 312 accessions reveals the local adaptation of foxtail millet.

Theor Appl Genet 2021 May 10;134(5):1303-1317. Epub 2021 Feb 10.

Beijing Agro-Biotechnology Research Center, Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.

Key Message: Based on the high-density variation map, we identified genome-level evidence for local adaptation and demonstrated that Siprr37 with transposon insertion contributes to the fitness of foxtail millet in the northeastern ecoregion. Adaptation is a robust way through which plants are able to overcome environmental constraints. The mechanisms of adaptation in heterogeneous natural environments are largely unknown. Deciphering the genomic basis of local adaptation will contribute to further improvement in domesticated plants. To this end, we describe a high-depth (19.4 ×) haplotype map of 3.02 million single nucleotide polymorphisms in foxtail millet (Setaria italica) from whole-genome resequencing of 312 accessions. In the genome-wide scan, we identified a set of improvement signals (including the homologous gene of OsIPA1, a key gene controlling ideal plant architecture) related to the geographical adaptation to four ecoregions in China. In particular, based on the genome-wide association analysis results, we identified the contribution of a pseudo-response regulator gene, SiPRR37, to heading date adaptation in foxtail millet. We observed the expression changes of SiPRR37 resulted from a key Tc1-Mariner transposon insertion in the first intron. Positive selection analyses revealed that SiPRR37 mainly contributed to the adaptation of northeastern ecoregions. Taken together, foxtail millet adapted to the northeastern region by regulating the function of SiPRR37, which sheds lights on genome-level evidence for adaptive geographical divergence. Besides, our data provide a nearly complete catalog of genomic variation aiding the identification of functionally important variants.
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http://dx.doi.org/10.1007/s00122-020-03760-4DOI Listing
May 2021

[Clinical efficacy and safety of different antiviral regimens in patients with coronavirus disease 2019].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2020 Dec;32(12):1423-1427

General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China.

Objective: To investigate the clinical efficacy and short-term and long-term adverse reactions for different antiviral regiments for coronavirus disease 2019 (COVID-19) in Ningxia Hui Autonomous Region during hospitalization and follow-up in 3 months.

Methods: A single-center retrospective study was conducted to enroll the COVID-19 patients in isolation ward of the only designated hospital to receive COVID-19 patients (the Fourth People's Hospital of Ningxia Hui Autonomous Region) when the authors were assigned by the Ningxia Health Commission as experts from January 20, 2020 to March 15, 2020. According to the antiviral regimen, the patients were divided into conventional antiviral group and unconventional antiviral group. The conventional antiviral group received α-interferon combined with Lopinavir/Ritonavir (LPV/R). The unconventional antiviral group was given α-interferon combined with LPV/R and Abidor or Ribavirin or Chloroquine. The patients were divided into mild (13 cases), ordinary (45 cases), severe (14 cases) and critical (1 case) types. The clinical data, length of hospital stay, the first 2019 novel coronavirus (2019-nCoV) nucleic acid negative recovery time, cost of hospitalization, 2019-nCoV nucleic acid positive reversal after 14 days of discharge, and the combination of hormones and antibiotics were collected. The differences in blood routine, liver function, blood lipid level and adverse reactions of antiviral drugs during hospitalization were compared between the two groups at 1, 3 and 7 days after admission and 1 and 3 months after discharge.

Results: (1) General information: a total of 75 patients with confirmed COVID-19 were admitted, and 73 patients were eventually enrolled, including 47 cases in the conventional antiviral group and 26 cases in the unconventional antiviral group. Patients with different clinical classification were analyzed, the higher the clinical classification and the patients' age, the higher the proportion of primary diseases and the cost of treatment, and the longer the length of hospital stay. Compared with conventional antiviral group, in unconventional antiviral group the percentage of severe and critical patients were higher [34.6% (9/26) vs. 10.6% (5/47), 3.8% (1/26) vs. 0 (0/47)], the length of hospital stay (days: 16.1±5.6 vs. 11.6±3.3), first nucleic acid negative recovery time (days: 12.4±4.5 vs. 10.0±3.5) were longer, and hospitalization cost was higher [Yuan: 11 984.2 (9 000.6, 24 424.7) vs. 8 140.4 (6 715.7,9 707.7)], with statistically significant differences (all P < 0.05). There were no significant differences in gender, age, proportion of patients with primary diseases and nucleic acid positive reversal rate after 14 days of discharge between the unconventional and conventional antiviral groups (all P > 0.05). (2) Laboratory tests: during the hospitalization, white blood cell count (WBC), platelet count (PLT), total bilirubin (TBil) and three acyl glycerin (TG) levels were first increased and then reduced, lymphocyte count (LYM) was first decreased and then increased in two groups. In the unconventional antiviral group, WBC [(6.53±2.78)×10/L], PLT [(250.77±96.12)×10/L], and TG [(1.94±0.96) μmol/L] all reached their peak values at 7 days after admission. TBil peaked at 3 days after admission, which was (23.69±12.14) μmol/L, and LYM reached the peak 1 month after discharge, which was (1.82±0.50)×10/L; however, there was no statistical significance among the above indicators between two groups. There were no statistically significant differences in alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total cholesterol (TC) between the two groups at each time point. (3) The ratio of combined use of hormones in the non-antiviral group was significantly higher than that in the conventional antiviral group [26.9% (7/26) vs. 4.3% (2/47), P < 0.05].

Conclusions: Age and associated primary diseases are related to the severity of COVID-19 patients. Unconventional antiviral treatment regimens are mostly used for severe COVID-19 patients whose ucleic acid did not turn negative for a long time. Individual antiviral therapy can be used based on the patients' response and tolerance to drugs.
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http://dx.doi.org/10.3760/cma.j.cn121430-20201019-00679DOI Listing
December 2020

An improved and efficient method of Agrobacterium syringe infiltration for transient transformation and its application in the elucidation of gene function in poplar.

BMC Plant Biol 2021 Jan 21;21(1):54. Epub 2021 Jan 21.

Beijing Agro-Biotechnology Research Center, Beijing Academy of Agricultural and Forestry Sciences, No. 9, Shuguang Huayuan Middle Road, Haidian District, Beijing, 100097, People's Republic of China.

Background: Forest trees have important economic and ecological value. As a model tree, poplar has played a significant role in elucidating the molecular mechanisms underlying tree biology. However, a lack of mutant libraries and time-consuming stable genetic transformation processes severely limit progress into the functional characterization of poplar genes. A convenient and fast transient transformation method is therefore needed to enhance progress on functional genomics in poplar.

Methods: A total of 11 poplar clones were screened for amenability to syringe infiltration. Syringe infiltration was performed on the lower side of the leaves of young soil-grown plants. Transient expression was evaluated by visualizing the reporters β-glucuronidase (GUS) and green fluorescent protein (GFP). The experimental parameters of the syringe agroinfiltration were optimized based on the expression levels of the reporter luciferase (LUC). Stably transformed plants were regenerated from transiently transformed leaf explants through callus-induced organogenesis. The functions of Populus genes in secondary cell wall-thickening were characterized by visualizing lignin deposition therein after staining with basic fuchsin.

Results: We greatly improved the transient transformation efficiency of syringe Agrobacterium infiltration in poplar through screening for a suitable poplar clone from a variety of clones and optimizing the syringe infiltration procedure. The selected poplar clone, Populus davidiana × P. bolleana, is amenable to Agrobacterium syringe infiltration, as indicated by the easy diffusion of the bacterial suspension inside the leaf tissues. Using this technique, we localized a variety of poplar proteins in specific intracellular organelles and illustrated the protein-protein and protein-DNA interactions. The transiently transformed leaves could be used to generate stably transformed plants with high efficiency through callus induction and differentiation processes. Furthermore, transdifferentiation of the protoxylem-like vessel element and ectopic secondary wall thickening were induced in the agroinfiltrated leaves via the transient overexpression of genes associated with secondary wall formation.

Conclusions: The application of P. davidiana × P. bolleana in Agrobacterium syringe infiltration provides a foundation for the rapid and high-throughput functional characterization of Populus genes in intact poplar plants, including those involved in wood formation, and provides an effective alternative to Populus stable genetic transformation.
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http://dx.doi.org/10.1186/s12870-021-02833-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818742PMC
January 2021

Genome structure variation analyses of peach reveal population dynamics and a 1.67 Mb causal inversion for fruit shape.

Genome Biol 2021 01 5;22(1):13. Epub 2021 Jan 5.

Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, People's Republic of China.

Background: Structural variations (SVs), a major resource of genomic variation, can have profound consequences on phenotypic variation, yet the impacts of SVs remain largely unexplored in crops.

Results: Here, we generate a high-quality de novo genome assembly for a flat-fruit peach cultivar and produce a comprehensive SV map for peach, as a high proportion of genomic sequence is occupied by heterozygous SVs in the peach genome. We conduct population-level analyses that indicate SVs have undergone strong purifying selection during peach domestication, and find evidence of positive selection, with a significant preference for upstream and intronic regions during later peach improvement. We perform a SV-based GWAS that identifies a large 1.67-Mb heterozygous inversion that segregates perfectly with flat-fruit shape. Mechanistically, this derived allele alters the expression of the PpOFP2 gene positioned near the proximal breakpoint of the inversion, and we confirm in transgenic tomatoes that PpOFP2 is causal for flat-fruit shape.

Conclusions: Thus, beyond introducing new genomics resources for peach research, our study illustrates how focusing on SV data can drive basic functional discoveries in plant science.
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http://dx.doi.org/10.1186/s13059-020-02239-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7784018PMC
January 2021

Efflux identification and engineering for ansamitocin P-3 production in Actinosynnema pretiosum.

Appl Microbiol Biotechnol 2021 Jan 4;105(2):695-706. Epub 2021 Jan 4.

State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

Ansamitocin P-3 (AP-3) exhibits potent biological activities against various tumor cells. As an important drug precursor, reliable supply of AP-3 is limited by low fermentation yield. Although different strategies have been implemented to improve AP-3 yield, few have investigated the impact of efflux on AP-3 production. In this study, AP-3 efflux genes were identified through combined analysis of two sets of transcriptomes. The production-based transcriptome was implemented to search for efflux genes highly expressed in response to AP-3 accumulation during the fermentation process, while the resistance-based transcriptome was designed to screen for genes actively expressed in response to the exogenous supplementation of AP-3. After comprehensive analysis of two transcriptomes, six efflux genes outside the ansamitocin BGC were identified. Among the six genes, individual deletion of APASM_2704, APASM_6861, APASM_3193, and APASM_2805 resulted in decreased AP-3 production, and alternative overexpression led to AP-3 yield increase from 264.6 to 302.4, 320.4, 330.6, and 320.6 mg/L, respectively. Surprisingly, APASM_2704 was found to be responsible for exportation of AP-3 and another macro-lactam antibiotic pretilactam. Furthermore, growth of APASM_2704, APASM_3193, or APASM_2805 overexpression mutants was obviously improved under 300 mg/L AP-3 supplementation. In summary, our study has identified AP-3 efflux genes outside the ansamitocin BGC by comparative transcriptomic analysis, and has shown that enhancing the transcription of transporter genes can improve AP-3 production, shedding light on strategies used for exporter screening and antibiotic production improvement. KEY POINTS: • AP-3-related efflux genes were identified by transcriptomic analysis. • Deletion of the identified efflux genes led in AP-3 yield decrease. • Overexpression of the efflux genes resulted in increased AP-3 production.
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http://dx.doi.org/10.1007/s00253-020-11044-6DOI Listing
January 2021

A highly sensitive, fast responsive and reversible naphthalimide-based fluorescent probe for hypochlorous acid and ascorbic acid in aqueous solution and living cells.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Feb 4;247:119138. Epub 2020 Nov 4.

School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou 221116, People's Republic of China. Electronic address:

It is very important to exploit real-time, ultrasensitive and specific visualization detection methods for hypochlorous acid/hypochlorite (HOCl/ClO) in biological systems as they are the guardians of the human immune system against pathogens invasion. In our work, we designed a novel reversible naphthalimide-based fluorescent probe NAP-OH to recognize HClO/ClO with a unique selective colorimetric and fluorescent response, a short response time (<8 s) and a high sensitivity (10.3 nM). In addition, NAP-OH exhibits a novel on-off-on fluorescence response to ClO/ascorbic acid (AA) with good cycle stability. The fluorescence signal is quenched because HClO/ClO oxidizes the subunit of NAP-OH to the segment 2,2,6,6-tetramethyl-1-oxo-piperidinium in NAP-O, which can be reduced by AA with the recovery of fluorescence. Finally, the confocal fluorescence imaging has been performed, which proves that NAP-OH can satisfactorily monitor intracellular endogenous and exogenous HClO/AA redox cycles.
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http://dx.doi.org/10.1016/j.saa.2020.119138DOI Listing
February 2021

An Efficient Resource Allocation Strategy for Edge-Computing Based Environmental Monitoring System.

Sensors (Basel) 2020 Oct 28;20(21). Epub 2020 Oct 28.

National Computer Network Emergency Response Technical Team, Coordination Center of China, Beijing 100096, China.

The cloud computing and microsensor technology has greatly changed environmental monitoring, but it is difficult for cloud-computing based monitoring system to meet the computation demand of smaller monitoring granularity and increasing monitoring applications. As a novel computing paradigm, edge computing deals with this problem by deploying resource on edge network. However, the particularity of environmental monitoring applications is ignored by most previous studies. In this paper, we proposed a resource allocation algorithm and a task scheduling strategy to reduce the average completion latency of environmental monitoring application, when considering the characteristic of environmental monitoring system and dependency among task. Simulations are conducted, and the results show that compared with the traditional algorithms. With considering the emergency task, the proposed methods decrease the average completion latency by 21.6% in the best scenario.
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http://dx.doi.org/10.3390/s20216125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662575PMC
October 2020

Many-body tunneling and nonequilibrium dynamics in double quantum dots with capacitive coupling.

J Phys Condens Matter 2020 Feb;33(7):075301

School of Microelectronics, Beihang University, Beijing 100191, People's Republic of China. Beihang-Goertek Joint Microelectronics Institute, Qingdao Research Institute, Beihang University, Qingdao 266104, People's Republic of China.

Double quantum dots (DQDs) systems may be the minimal setups for realization of QD-based qubits and quantum computation. Pauli spin blockade (PSB) and a kind of novel many-body tunneling (MBT) are identified to play important roles in these systems, and dominate the quantum tunneling at moderate and weak interdot coupling t, respectively. On the other hand, inter-dot Coulomb interaction U' and related inter-dot Coulomb blockade (IDCB) is inevitable in DQDs. However, what would happen on the effect of U' in DQDs has not been touched, in particular for PSB and MBT. Here, we study the tunneling processes and transport properties with various U' in series-coupled DQDs, and find MBT process is rather robust against U' within U'/U < 0.1, where U is the intra-dot Coulomb interaction. Meanwhile, the linearity relationship between the carrier doublon number and MBT current remains valid. These findings enrich the understanding of the many-body tunneling in the DQDs and may shed light on the manipulation of the QD-based qubits.
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http://dx.doi.org/10.1088/1361-648X/abc5d5DOI Listing
February 2020

Primary First Bite Syndrome of the Parotid Gland: Case Report and Literature Review.

Ear Nose Throat J 2020 Oct 6:145561320962584. Epub 2020 Oct 6.

Department of Oral and Maxillofacial Surgery, State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, 12644Fourth Military Medical University, Xi'an, Shaanxi Province, China.

Objective: A case of primary first bite syndrome (FBS), diagnosed in a patient with nonspecific adenocarcinoma of the deep lobe of the parotid gland.

Data Sources: A Medline literature search was conducted on PubMed, using the keywords "first bite syndrome."

Review Methods: Using primary FBS and existence of a definite etiology as inclusion criteria.

Results: We report on an unusual case of primary FBS, which had no surgical history. After multiple examinations, the pain was localized to a mass in the deep lobe of the parotid gland. After tumorectomy, the FBS pain was significantly relieved. The postoperative pathological examination determined that the excised mass was a nonspecific adenocarcinoma. Reviewing the literature, we found that primary FBS was mostly caused by malignant tumors in the inferior temporal fossa, the deep lobe of the parotid gland, and (or) the parapharyngeal space. Surgery was reported to be an effective treatment.

Conclusion: The case highlights the critical importance of identifying the etiology of primary FBS. When manifested with a primary FBS, malignant tumors must be high on the differential diagnosis list, especially those in the region of the inferior temporal fossa, the deep lobe of the parotid gland, and the parapharyngeal space.
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http://dx.doi.org/10.1177/0145561320962584DOI Listing
October 2020

A pair of new isocoumarin enantiomers of .

Nat Prod Res 2020 Sep 3:1-8. Epub 2020 Sep 3.

School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China.

A pair of new isocoumarin derivative enantiomers, ()-(-)-3-(3,3-dichloro-2-hydroxy-propyl)-4-chlorine-6,8-dihydroxy-isochromen-1- one () and ()-(+)-3-(3,3-dichloro-2-hydroxy-propyl)-4-chlorine-6,8-dihydroxy-isochromen-1- one (), as well as seven known compounds (-) were isolated from . Compounds and were confirmed to be a pair of enantiomers by chiral HPLC-CD analysis, and the structure of compound was determined by spectroscopic analyses including extensive 1 D (H NMR, C NMR) and 2 D NMR spectra (COSY, HSQC and HMBC) and MS data. And the absolute configurations of compounds and were determined by the quantum chemical ECD calculations. Compounds - are firstly reported from this plant. In the assays, compounds and can inhibit human laryngeal cancer Hep-2 cell line growth in a dose- and time-dependent manner. In addition, compounds and have effects on increasing glucose uptake . Compound showed a strong glucose uptake in L6 cells, with enhancements by 1.8 folds.
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http://dx.doi.org/10.1080/14786419.2020.1815738DOI Listing
September 2020

Applying nomograms based on the surveillance, epidemiology and end results database to predict long-term overall survival and cancer-specific survival in patients with oropharyngeal squamous cell carcinomas: A case-control research.

Medicine (Baltimore) 2020 Jul;99(30):e20703

State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery School of Stomatology, The Fourth Military Medical University, Xi'an, China.

Few models regarding to the individualized prognosis assessment of oropharyngeal squamous cell carcinoma (OPSCC) patients were documented. The purpose of this study was to establish nomogram model to predict the long-term overall survival (OS) and cancer-specific survival (CSS) of OPSCC patients. The detailed clinical data for the 10,980 OPSCC patients were collected from the surveillance, epidemiology and end results (SEER) database. Furthermore, we applied a popular and reasonable random split-sample method to divide the total 10,980 patients into 2 groups, including 9881 (90%) patients in the modeling cohort and 1099 (10%) patients in the external validation cohort. Among the modeling cohort, 3084 (31.2%) patients were deceased at the last follow-up date. Of those patients, 2188 (22.1%) patients died due to OPSCC. In addition, 896 (9.1%) patients died due to other causes. The median follow-up period was 45 months (1-119 months). We developed 2 nomograms to predict 5- and 8- year OS and CSS using Cox Proportional Hazards model. The nomograms' accuracy was evaluated through the concordance index (C-index) and calibration curves by internal and external validation. The C-indexes of internal validation on the 5- and 8-year OS and CSS were 0.742 and 0.765, respectively. Moreover, the C-indexes of external validation were 0.740 and 0.759, accordingly. Based on a retrospective cohort from the SEER database, we succeeded in constructing 2 nomograms to predict long-term OS and CSS for OPSCC patients, which provides reference for surgeons to develop a treatment plan and individual prognostic evaluations.
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http://dx.doi.org/10.1097/MD.0000000000020703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7386992PMC
July 2020

Current-induced effective Dzyaloshinskii-Moriya interaction and its Kondo enhancement in double quantum dot.

J Chem Phys 2020 Apr;152(16):164113

Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui 230026, China.

We studied the nonequilibrium transport of serially coupled double quantum dots connected to ferromagnetic electrodes. We demonstrated that the nonadiabatic part of the spin gauge field resulted in a current-induced Dzyaloshinskii-Moriya (DM) interaction effect in a double quantum dot and numerically confirmed this observation through the hierarchical equations of motion approach. We report that the spin current and the effective DM interaction are enhanced in the Kondo regime. We demonstrate that this enhancement occurs because the Kondo resonance, which is supposed to be suppressed by the local ferromagnetic exchange, is enhanced by the inter-dot coupling. This additional Kondo resonance channel increases the spin current. In addition, the impact of the spin-spin interaction and the Kondo effect on tunnel magnetoresistance is discussed. Our results offer a new approach for controlling the non-collinear spin interaction in double quantum dot devices.
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http://dx.doi.org/10.1063/1.5144624DOI Listing
April 2020

Contingency management strategies of the Nursing Department in centralized rescue of patients with coronavirus disease 2019.

Int J Nurs Sci 2020 Apr 4;7(2):139-142. Epub 2020 Apr 4.

The Department of Hepatopancreatobiliary Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Objectives: This article aims to summarize a series of contingency management strategies of the Nursing Department in the centralized treatment of patients with coronavirus disease 2019 (COVID-19).

Methods: The strategies of the Nursing Department included an early warning for prevention and control, taking functions of vertically commanding and horizontally coordinating, and reasonably allocating nursing workforce, to facilitate centralized treatment work in the in-hospital fever clinic, isolation wards and ICU, and referral and admission of critical patients. Five special groups were established in charge of training and examination, management and supervision, psychological support, logistical support, and reporting and publicity, respectively.

Results: It was achieved that no deaths from critical patients and no medical staff, no other patients were infected.

Conclusion: Through the implementation of these strategies, safe and efficient centralized treatment was ensured timely, orderly and sustainably.
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http://dx.doi.org/10.1016/j.ijnss.2020.04.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129619PMC
April 2020

Emerging crosstalk between two signaling pathways coordinates K+ and Na+ homeostasis in the halophyte Hordeum brevisubulatum.

J Exp Bot 2020 07;71(14):4345-4358

Beijing Agro-biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

K+/Na+ homeostasis is the primary core response for plant to tolerate salinity. Halophytes have evolved novel regulatory mechanisms to maintain a suitable K+/Na+ ratio during long-term adaptation. The wild halophyte Hordeum brevisubulatum can adopt efficient strategies to achieve synergistic levels of K+ and Na+ under high salt stress. However, little is known about its molecular mechanism. Our previous study indicated that HbCIPK2 contributed to prevention of Na+ accumulation and K+ reduction. Here, we further identified the HbCIPK2-interacting proteins including upstream Ca2+ sensors, HbCBL1, HbCBL4, and HbCBL10, and downstream phosphorylated targets, the voltage-gated K+ channel HbVGKC1 and SOS1-like transporter HbSOS1L. HbCBL1 combined with HbCIPK2 could activate HbVGKC1 to absorb K+, while the HbCBL4/10-HbCIPK2 complex modulated HbSOS1L to exclude Na+. This discovery suggested that crosstalk between the sodium response and the potassium uptake signaling pathways indeed exists for HbCIPK2 as the signal hub, and paved the way for understanding the novel mechanism of K+/Na+ homeostasis which has evolved in the halophytic grass.
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http://dx.doi.org/10.1093/jxb/eraa191DOI Listing
July 2020

Theoretical Study on the Effect of Environment on Excitation Energy Transfer in Photosynthetic Light-Harvesting Systems.

J Phys Chem B 2020 03 16;124(12):2354-2362. Epub 2020 Mar 16.

Department of Physics & Beijing Key Laboratory of Optoelectronic Functional Materials and Micro-nano Devices, Renmin University of China, Beijing 100872, China.

In recent years, two-dimensional (2D) electronic spectroscopy experiments prove that the excitation energy transfer (EET) in photosynthetic light-harvesting systems presents long-lived electronic quantum beating signals. After being discovered in the light-harvesting system, the quantum coherence effect has aroused widespread discussion. To illustrate the EET process in the Fenna-Matthews-Olson (FMO) and phycocyanin 645 (PC645) complex, the local protein environment is often thought to be the same; however, this is ambivalent to the practical structural analysis of the light-harvesting complex. By adopting the dissipaton equation of motion theory, we present the effect of a heterogeneous protein environment on the energy transfer process with accurate numerical results. We demonstrate that the energy transfer process relies on the local heterogeneous environment for the FMO complex. A similar good agreement is found for the PC645 complex. Furthermore, we discuss the optimal value of different chromophores in the excitation energy transfer process by controlling the environmental characteristics.
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http://dx.doi.org/10.1021/acs.jpcb.0c00266DOI Listing
March 2020

Molecular Design, Synthesis and Docking Study of Alkyl and Benzyl Derivatives of Robustic Acid as Topoisomerase I Inhibitors.

Chem Biodivers 2020 Mar 14;17(3):e1900556. Epub 2020 Feb 14.

Guangxi Medical University, Nanning, 530021, P. R. China.

Robustic acid is reported to be a bioactive compound, isolated from the medicinal plant Dalbergia benthamii Prain. Ten alkyl and benzyl derivatives (2a-2j) of robustic acid were designed and synthesized based on molecular docking approaches. The biological activities of most of the synthesized compounds (such as 2g, 2h, and 2i) were closely consistent with the docking results. In particular, 4-O-phenylpropyl substituted compound 2g displayed potent topoisomerase I inhibitory activity as well as cytotoxicity against SMMC-7721, HepG2, and HeLa cell lines. Further biological testing suggests that compound 2g acted mainly by an arrest of the tumor cells in G1 phase of the cell cycle and suppressed cell proliferation by inducing apoptosis. The findings of this study are encouraging with respect to potential utilization of these compounds as new topoisomerase I inhibitors.
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http://dx.doi.org/10.1002/cbdv.201900556DOI Listing
March 2020

NT-3/TrkC Axis Contributes to the Perineural Invasion and the Poor Prognosis in Human Salivary Adenoid Cystic Carcinoma.

J Cancer 2019 15;10(24):6065-6073. Epub 2019 Oct 15.

State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases, and Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.

The present study was aimed to investigate the role and mechanism of neurotrophin-3 (NT-3) and its specific receptor tropomyosin receptor kinase C (TrkC) in the perineural invasion (PNI) process of the salivary adenoid cystic carcinoma (SACC). The co-cultured system between SACC cells and Schwann cells (SCs) was employed to detect the expression of NT-3 and TrkC. The results of ELISA, qRT-PCR and western blot showed that NT-3 was noticeably elevated in the co-cultured SACC-83 cells, while TrkC was increased in the co-cultured SCs. The results of scratch wound healing, migration, and 3D co-culture assays showed that the directional migration abilities of the co-cultured SACC-83 cells and SCs were significantly increased. Under the stimulation of NT-3, the directional motor ability of SACC-83 cells and SCs was significantly improved, and the apoptosis of SACC-83 cells and SCs were obviously inhibited. In addition, blocking TrkC by its specific inhibitor AZD7451 could significantly inhibit these effects. Immunohistochemistry staining showed that the positive expression of NT-3 (88.5%) and TrkC (92.3%) was significantly correlated with the PNI in SACC specimens ( < 0.05). Additionally, the high expression of NT-3 was significantly associated with the poor prognosis of SACC patients ( < 0.05). The present study indicated that NT-3/TrkC axis contributed to the PNI progression and the poor prognosis of SACC via regulating the interaction between SACC cells and SCs. Interruption of the interaction between SACC cells and SCs by blocking the NT-3/TrkC axis might be an effective strategy for anti-PNI therapy in SACC.
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http://dx.doi.org/10.7150/jca.33635DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6856580PMC
October 2019

Synthesis and Evaluation of Anticancer Activity of New 4-Acyloxy Derivatives of Robustic Acid.

Int J Mol Sci 2019 Oct 26;20(21). Epub 2019 Oct 26.

College of Pharmacy, Guangxi Medical University, Nanning 530021, China.

In the present study, a series of 4-acyloxy robustic acid derivatives were synthesized and characterized for evaluation of their anti-cancer activity. The structures of these derivatives were elucidated by mass spectra (MS) nuclear magnetic resonance spectra (NMR). The single-crystal X-ray diffraction structure of one of these compounds was obtained, for further validation of the target compound structures. The anticancer activities of the target products were evaluated against human leukemic cells HL-60, human non-small cell lung carcinoma cells A-549, human hepatic carcinoma cells SMMC-7721, human hepatocellular carcinoma cells HepG2, and human cervical carcinoma cells Hela. Three compounds among them exhibited potent in-vitro cytotoxicity and excellent DNA topoisomerase I inhibitory activity, even at 0.1 mM concentrations. The most noteworthy observation was the minor toxicity of two of these compounds to normal cells, with an activity similar to the positive control in cancerous cells. A Surflex-Dock docking study was performed to investigate the topoisomerase I activity of all compounds. Of all the other compounds, the most sensitive compound was selected for further investigation of its effect on apoptosis induction and cell cycle regulation in HL-60 cells. Our results suggest that the anticancer effects of these compounds can be attributed to their pharmacological effects on topoisomerase I, cell apoptosis, and cell cycle. These findings suggest that robustic acid derivatives could be used as potential antitumor drugs.
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http://dx.doi.org/10.3390/ijms20215336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862253PMC
October 2019

PmiREN: a comprehensive encyclopedia of plant miRNAs.

Nucleic Acids Res 2020 01;48(D1):D1114-D1121

Beijing Agro-biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, P. R. China.

MicroRNAs (miRNAs) are small non-coding RNA molecules that function as diverse endogenous gene regulators at the post-transcriptional level. In the past two decades, as research effort on miRNA identification, function and evolution has soared, so has the demand for miRNA databases. However, the current plant miRNA databases suffer from several typical drawbacks, including a lack of entries for many important species, uneven annotation standards across different species, abundant questionable entries, and limited annotation. To address these issues, we developed a knowledge-based database called Plant miRNA Encyclopedia (PmiREN, http://www.pmiren.com/), which was based on uniform processing of sequenced small RNA libraries using miRDeep-P2, followed by manual curation using newly updated plant miRNA identification criteria, and comprehensive annotation. PmiREN currently contains 16,422 high confidence novel miRNA loci in 88 plant species and 3,966 retrieved from miRBase. For every miRNA entry, information on precursor sequence, precursor secondary structure, expression pattern, clusters and synteny in the genome, potential targets supported by Parallel Analysis of RNA Ends (PARE) sequencing, and references is attached whenever possible. PmiREN is hierarchically accessible and has eight built-in search engines. We believe PmiREN is useful for plant miRNA cataloguing and data mining, therefore a resource for data-driven miRNA research in plants.
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http://dx.doi.org/10.1093/nar/gkz894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943064PMC
January 2020

CCL2/CCR2 Axis Promotes the Progression of Salivary Adenoid Cystic Carcinoma via Recruiting and Reprogramming the Tumor-Associated Macrophages.

Front Oncol 2019 9;9:231. Epub 2019 Apr 9.

State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, Xi'an, China.

The present study investigated the roles and underlying mechanism of CCL2/CCR2 axis in the interactions between tumor cells and tumor-associated macrophages (TAMs) during the progression of salivary adenoid cystic carcinoma (SACC). Immunohistochemical staining and survival analysis were performed to study the correlation and clinical value of CD68, CD163, CCL2, and CCR2 expression in SACC cases. CCL2 silencing by RNA interference and CCR2 blocking by CCR2 specific antagonist (RS504393) were performed. ELISA, qRT-PCR, western blot, immunofluorescence, flow cytometry, CCK8, scratch wound healing, and transwell assays were used to explore the functional roles and possible mechanism of CCL2/CCR2 axis in the interactions between SACC cells and TAMs. The effects of targeting TAMs by blocking the CCL2/CCR2 axis were investigated in a xenograft mice model with SACC cells. The high infiltration of TAMs marked by CD68 and high infiltration of M2 TAMs marked by CD163 were significantly correlated with the expression of CCL2 and CCR2 in SACC tissues. Notably, the high infiltration of TAMs and the overexpression of CCL2 were obviously associated with the clinical progression and poor prognosis of SACC. SACC cells derived CCL2 could activate its receptor CCR2 expression in TAMs . The results further indicated that the SACC cells derived CCL2 was involved in the recruitment, M2 polarization, and GDNF expression of TAMs through the CCL2/CCR2 axis. Meanwhile, TAMs derived GDNF promoted the proliferation, migration, and invasion of SACC cells through the GDNF/p-RET pathway. Treating immunodeficient mice with the CCR2 antagonist (RS504393) greatly inhibited the infiltration of TAMs and the tumorigenicity of SACC cells. These new findings indicated that the CCL2/CCR2 axis promoted the progression of SACC cells via recruiting and reprogramming TAMs. Targeting TAMs by blocking the CCL2/CCR2 axis might be a prospective strategy for SACC therapy.
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http://dx.doi.org/10.3389/fonc.2019.00231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6465613PMC
April 2019
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