Publications by authors named "Jianhua Deng"

22 Publications

  • Page 1 of 1

Comparison of analgesic activities of aconitine in different mice pain models.

PLoS One 2021 1;16(4):e0249276. Epub 2021 Apr 1.

Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of China, International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.

Aconitine (AC) is the primary bioactive and secondary metabolite alkaloidin of Aconitum species which is accounted for more than 60% of the total diester-diterpenoid alkaloids in Aconite. To evaluate the analgesic effects of AC, 4 different pain models including hot plate assay, acetic acid writhing assay, formalin and CFA induced pain models were adopted in this study. In hot plate experiment, AC treatment at concentration of 0.3 mg/kg and 0.9 mg/kg improved the pain thresholds of mice similar to the positive drug aspirin at the concentration of 200 mg/kg (17.12% and 20.27% VS 19.21%). In acetic acid writhing experiment, AC significantly reduced the number of mice writhing events caused by acetic acid, and the inhibition rates were 68% and 76%. These results demonstrated that AC treatment revealed significant analgesic effects in both acute thermal stimulus pain model and chemically-induced visceral pain model. The biphasic nociceptive responses induced by formalin were significantly inhibited after AC treatment for 1h or 2h. The inhibition rates were 33.23% and 20.25% of AC treatment for 1h at 0.3 mg/kg and 0.9 mg/kg in phase I. In phase II, the inhibition rates of AC and aspirin were 36.08%, 32.48% and 48.82% respectively, which means AC showed similar analgesic effect to non-steroidal anti-inflammatory compounds. In the chronic CFA-induced nociception model, AC treatment also improved mice pain threshold to 131.33% at 0.3 mg/kg, which was similar to aspirin group (152.03%). Above all, our results verified that AC had obviously analgesic effects in different mice pain models.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249276PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016268PMC
September 2021

Scleral concave pool trabeculectomy combined phacoemulsification in primary open-angle glaucoma with cataract.

BMC Ophthalmol 2020 Jun 9;20(1):222. Epub 2020 Jun 9.

Guangdong Hospital of Traditional Chinese Medicine, Zhuhai, China.

Background: To investigate the safety and efficacy of scleral concave pool trabeculectomy (SCPT) combined phacoemulsification for eyes with coexisting cataract and primary open-angle glaucoma (POAG).

Methods: This was a retrospective, controlled, interventional pilot case series. Thirty patients (30 eyes) were diagnosed with coexisting cataract and POAG between May 2015 and April 2018. Fourteen eyes underwent SCPT combined phacoemulsification were set as the study group, and 16 eyes received conventional phacotrabeculectomy were set as the control group. All patients were followed up for at least 6 months. The preoperative to postoperative changes in IOP, glaucoma medication requirements, BCVA, blebs functions, and adverse events were recorded.

Results: The groups were matched for baseline age, BCVA, IOP and types of IOP-lowering medications (all P > 0.05). At 6-month visit, there were no significant difference between control and study group in the improvement of BCVA (0.22 ± 0.24 versus 0.18 ± 0.26, P = 0.718), reduction of IOP (- 11.21 ± 8.61 mmHg versus - 9.19 ± 9.18 mmHg, P = 0.540) and the number of eyes that needed IOP-lowering medications (2 versus 3, P = 0.743). At the last visit, the rate of forming functioning blebs was significantly different between the study and control groups, (92.9% versus 68.7% respectively, P = 0.007). In the study group, 5 eyes developed hypotony, and 1 eye showed limited choroidal detachment, whereas in the control group 1 eye developed malignant glaucoma. All adverse events were successfully managed.

Conclusion: The SCPT combined phacoemulsification seems to be a safe and effective alternative to conventional phacotrabeculectomy for patients with POAG and visually significant cataract in the short-term.
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http://dx.doi.org/10.1186/s12886-020-01500-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285585PMC
June 2020

Nickel Hollow Spheres Concatenated by Nitrogen-Doped Carbon Fibers for Enhancing Electrochemical Kinetics of Sodium-Sulfur Batteries.

Adv Sci (Weinh) 2020 Feb 23;7(4):1902617. Epub 2019 Dec 23.

Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University) Ministry of Education School of Materials and Energy Southwest University Chongqing 400715 P. R. China.

The high energy density of room temperature (RT) sodium-sulfur batteries (Na-S) usually rely on the efficient conversion of polysulfide to sodium sulfide during discharging and sulfur recovery during charging, which is the rate-determining step in the electrochemical reaction process of Na-S batteries. In this work, a 3D network (Ni-NCFs) host composed by nitrogen-doped carbon fibers (NCFs) and Ni hollow spheres is synthesized by electrospinning. In this novel design, each Ni hollow unit not only can buffer the volume fluctuation of S during cycling, but also can improve the conductivity of the cathode along the carbon fibers. Meanwhile, the result reveals that a small amount of Ni is polarized during the sulfur-loading process forming a polar Ni-S bond. Furthermore, combining with the nitrogen-doped carbon fibers, the Ni-NCFs composite can effectively adsorb soluble polysulfide intermediate, which further facilitates the catalysis of the Ni unit for the redox of sodium polysulfide. In addition, the in situ Raman is employed to supervise the variation of polysulfide during the charging and discharging process. As expected, the freestanding [email protected] cathode exhibits outstanding rate capability and excellent cycle performance.
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http://dx.doi.org/10.1002/advs.201902617DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029643PMC
February 2020

Inhibition of MicroRNA-9 Improves Fracture Healing by Modulating the Bone Morphogenetic Protein-7 Pathway.

Pharmacology 2019 16;104(5-6):352-358. Epub 2019 Oct 16.

Department of Orthopaedic Surgery, Shanghai Tenth People's Hospital, Shanghai, China,

We evaluated the effect of microRNA (miR)-9 inhibition on fracture healing in a rat model of femoral fracture. The rats were divided into sham, negative control and miR-9 inhibitor groups. The miR-9 inhibitor group received 30 pmol/mL inhibitor intrathecally for 8 consecutive weeks following surgery-induced femoral fracture. The effect of miR-9 inhibition on fracture healing was estimated by determining the bone mineral density (BMD) and by performing X-ray analysis of the fractured bone. The serum levels of markers of bone formation were estimated by enzyme-linked immunosorbent assay and reverse transcription polymerase chain reaction, and western blotting and immunohistochemical analysis were performed to assess the effect of miR-9 inhibition on fracture healing. The BMD at the fracture site was significantly higher in the miR-9 inhibitor group than in the negative control group. Inhibition of miR-9 blocked the fracture gap and resulted in new callus formation at the fracture site. The serum levels of osteocalcin and bone GLA protein were increased and that of alkaline phosphatase was decreased by inhibition of miR-9 compared to levels in the negative control. However, inhibition of miR-9 significantly increased the mRNA levels of runt-related transcription factor 2 (Runx2) and bone morphogenetic protein 7 (BMP-7) in the bone tissue at the fracture site compared to the negative control group; this result was confirmed by western blotting. In conclusion, -miR-9 inhibition enhanced fracture healing by modulating the BMP-7/Runx2 signalling pathway in a rat model of femoral fracture.
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http://dx.doi.org/10.1159/000502402DOI Listing
May 2020

An eight-long non-coding RNA signature as a candidate prognostic biomarker for bladder cancer.

Aging (Albany NY) 2019 09 3;11(17):6930-6940. Epub 2019 Sep 3.

State Key Laboratory of Cancer Biology and Experimental Teaching Center of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.

Backgroud: Bladder cancer (BLCA) is one of the most fatal types of cancer worldwide. However, there are limited methods for us to provide a prognostic prediction of BLCA patients. Therefore, we aimed at developing a lncRNA signature to improve the prognosis prediction of BLCA.

Results: An eight-lncRNA signature was significantly associated with recurrence free survival in BLCA patients from both discovery and validation groups. Furthermore, genes involved in the signature were enriched in extracellular matrix organization pathway. Finally, functional experiments demonstrated that six out of the eight lncRNAs significantly regulated the invasion of BLCA cells.

Method: A total of 343 BLCA patients from The Cancer Genome Atlas (TCGA) were employed and randomly divided into training (n=172) and validating (n=171) groups. The lncRNA expression profiles of BLCA patients were screened and a risk-score formula were created and validated according to the Cox regression analysis. Next, WGCNA method was employed to cluster genes that highly correlated with the risk scores based on the profiling data of TCGA dataset and transwell assay was also performed to further investigate the role of these lncRNAs.

Conclusions: Our results suggested that the eight-lncRNA signature was a candidate prognostic biomarker for predicting tumor recurrence of patients with BLCA.
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http://dx.doi.org/10.18632/aging.102225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6756879PMC
September 2019

Rechargeable K-Se batteries based on metal-organic-frameworks-derived porous carbon matrix confined selenium as cathode materials.

J Colloid Interface Sci 2019 Mar 22;539:326-331. Epub 2018 Dec 22.

Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, Faculty of Materials and Energy, Southwest University, Chongqing 400715, PR China. Electronic address:

In this work, porous carbon matrix derived from metal-organic frameworks is synthesized by a facile carbonation process for confining element selenium. The Se/nitrogen-doped porous carbon composite is applied as the cathode for rechargeable K-Se batteries for the first time. The abundant and hierarchical porous structure is advantageous in overcoming the volume expansion problem caused by polyselenides during discharge-charge process. The in-situ nitrogen-doped porous carbon enhances electronic conductivity of the cathode composite material. The electrochemical result shows that the as-obtained Se/nitrogen-doped porous carbon composite with Se content of 53% delivers good rate capacity with coulombic efficiency of nearly ∼90% and a reversible cycling capacity of 327 mA h g at 0.2 C, maintaining about 130 mA h g even after 100 cycles.
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http://dx.doi.org/10.1016/j.jcis.2018.12.083DOI Listing
March 2019

[Comparison of combined intravenous and topical use of tranexamic acid with different dosage in primary total knee arthroplasty].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2018 11;32(11):1397-1401

Department of Orthopaedics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, 200233,

Objective: To compare the efficacy and safety of intra-articular combined with intravenous administration of tranexamic acid (TXA) with different dosage for reducing blood loss in primary total knee arthroplasty (TKA).

Methods: Between January 2017 and June 2017, 90 patients suffering from unilateral osteoarthritis who underwent primary TKA were randomly scheduled to three interventions, named groups A, B, and C. Single dosage of TXA via intravenous injection (IV) and different dosages of TXA via intra-articular injection (IA) were utilized in three groups, respectively. All patients in three groups received 1 g TXA IV at 10 minutes preoperatively, and received 1, 2, and 3 g TXA IA diluted in 50 mL saline after wound closure in groups A, B and C, respectively. The age, gender, body mass index, affected side of the knee, grade of osteoarthritis, grade of America Society of Anesthesiologist, preoperative hemoglobin (Hb) concentration, platelet count, preoperative prothrombin time, and activated partial thromboplastin time were not significantly different between groups ( >0.05). The postoperative wound blood drainage, Hb concentration at 1, 3, and 7 days after operation, transfusion rate, and thromboembolic complications were observed. All patients were routinely observed for deep vein thrombosis (DVT) by the color Doppler ultrasonography at 1 week, 1 month, and 3 months after operation, and the symptomatic pulmonary embolism (PE) were observed.

Results: All patients in three groups were followed up 7-12 months (mean, 8.4 months). There was no significant difference in operation time between groups ( >0.05). The postoperative wound blood drainage was significantly less in groups B and C than that in group A ( <0.05), whereas no significant difference was found between group B and group C ( >0.05). Incision skin necrosis occurred in 1 case of group B and fat liquefaction occurred in 1 case of group C. The other incisions of 3 groups healed by first intention. There was no significant difference in incision complication incidence between groups. The Hb concentration was significantly higher in groups B and C than that in group A at 1, 3, and 7 days after operation ( <0.05). While between group B and group C, the significant difference of Hb concentration only existed at 1 day after operation ( <0.05). The number of patients who got blood transfusion was significantly less in group B (4 cases, 13.3%) and group C (5 cases, 16.7%) than that in group A (9 cases, 30%) ( < 0.05), but no significant difference was found between group B and group C ( >0.05). The result of color Doppler ultrasonography showed that 1 case got DVT in the contralateral calf at 3 weeks in group B. And there was no symptomatic PE in 3 groups.

Conclusion: Combined administration of IV and IA TXA in a clinically relevant reduction in blood loss was effective and safe in primary TKA, and no thromboembolic complication was observed. The combination of 1 g IV with 2 g IA could be the optional choice.
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http://dx.doi.org/10.7507/1002-1892.201805008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414127PMC
November 2018

Enhanced cell growth on 3D graphene scaffolds implanted with nitrogen ions.

Biointerphases 2018 05 16;13(4):041001. Epub 2018 May 16.

Tianjin Institute of Urological Surgery, Tianjin Medical University, Tianjin 300070, China.

One of the key challenges in engineering tissues for cell-based therapies is developing biocompatible scaffold materials to direct cell behavior. In this paper, the cytocompatibilities of a flexible three-dimensional graphene scaffold (3D-G) and the same scaffold implanted with nitrogen ions (N/3D-G) are compared using an in vitro assay based on 3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. The N/3D-G samples were prepared from low-temperature hydrothermally synthesized flexible 3D-G by ion implantation and were found to display improved adhesion and proliferation of rat osteoblast and mouse fibroblast cells. In particular, the N/3D-G sample with a nitrogen content of ∼10% showed the highest levels of cell viability and proliferation. The flexible N/3D-G has potential applications as a biocompatible scaffold material that provides improved surface area and hydrophilic groups for cell growth and proliferation.
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http://dx.doi.org/10.1116/1.5025534DOI Listing
May 2018

Protective Effect of Pogostone on 2,4,6-Trinitrobenzenesulfonic Acid-Induced Experimental Colitis via Inhibition of T Helper Cell.

Front Pharmacol 2017 17;8:829. Epub 2017 Nov 17.

Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, China.

Inflammatory bowel disease (IBD) is a chronic immune-related disease mainly caused by the disequilibrium of T helper (Th) cell paradigm? Pogostone (PO) is one of the major chemical constituents of (Blanco) Benth. The present study aims to investigate the potential benefit of PO against IBD in a 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced experimental colitis model. PO treatment by enema significantly brought down the disease activity index (DAI) of the TNBS-challenged rats, which was manifested by the ameliorated inflammatory features including ulceration, adhesion, and edema. Hematoxylin-eosin (HE) staining and immunohistochemistry analysis showed that PO effectively relived colon damage by restoring epithelium, and more importantly, by inhibiting the infiltration of pro-inflammatory Th1 and Th17 cells in the colon. Additionally, PO inhibited the activity of myeloperoxidase and secretion of inflammatory cytokines including IFN-γ, IL-12p70, IL-17A, and IL-10. Together with our previous findings, the present data indicated that the anti-IBD effect of PO probably related to its direct inhibition on Th cell proliferation and suppression of the cytokines secretion. These results highlighted the potential of PO as a promising candidate to relieve IBD.
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http://dx.doi.org/10.3389/fphar.2017.00829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5699238PMC
November 2017

Associations Between TGFA/TGFB3/MSX1 Gene Polymorphisms and Congenital Non-Syndromic Hearing Impairment in a Chinese Population.

Med Sci Monit 2016 Jun 29;22:2253-66. Epub 2016 Jun 29.

Department of Otorhinolaryngology, The First People's Hospital Of Kunshan, Kunshan, Jiangsu, China (mainland).

BACKGROUND The aim of this study was to investigate whether the TGFA/TGFB3/MSX1 gene polymorphisms and haplotypes lead to individual differences between congenital non-syndromic hearing impairment (NSHI) patients and normal people in a Chinese population and to analyze the risk factors for NSHI. MATERIAL AND METHODS Between December 2010 and September 2014, 343 congenital NSHI patients were recruited as cases, and 272 healthy subjects were recruited as controls. Denaturing high-performance liquid chromatography (DHPLC) was used to identify genotypes, SHEsis software was used to conduct gene linkage disequilibrium and haplotype analyses, and regression analysis was performed to identify risk factors for congenital NSHI. RESULTS The distribution of genotype frequencies and allele frequencies of TGFA rs3771494, TGFB3 rs3917201 and rs2268626, and MSX1 rs3821949 and rs62636562 were significantly different between the case and the control groups (all P<0.05). TGFA/TGFB3/MSX1 gene rs3771494, rs1058213, rs3917201, rs2268626, rs3821949, and rs62636562 haplotype analysis showed that haplotype CCGTAC and TTACGT might be protective factors (both P<0.001), while TTGCGC might be a risk factor for the normal population (P<0.001). The other risk factors include paternal smoking, advanced maternal age, maternal sickness history, maternal contact with pesticides or similar drugs, maternal abortion history, maternal medication history, maternal passive smoking history during pregnancy, rs3771494 CT, rs2268626 CC and TC, and rs3821949 GG and AG genotypes were risk factors (all P<0.05), while maternal vitamin supplements during pregnancy, rs3917201 GA, rs62636562 TT and CT genotypes were protective factors for congenital NSHI (all P<0.05). CONCLUSIONS rs3771494, rs3917201, rs2268626, rs3821949 and rs62636562 might be associated with congenital NSHI.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4930271PMC
http://dx.doi.org/10.12659/msm.896527DOI Listing
June 2016

Clonality analysis of multifocal papillary thyroid carcinoma by using genetic profiles.

J Pathol 2016 May 1;239(1):72-83. Epub 2016 Apr 1.

Department of Head and Neck Surgery, Peking University Cancer Hospital and Institute, 52 Fucheng Road, Beijing, China.

Papillary thyroid carcinoma (PTC) is the most common adult thyroid malignancy and often presents with multiple anatomically distinct foci within the thyroid, known as multifocal papillary thyroid carcinoma (MPTC). The widespread application of the next-generation sequencing technologies in cancer genomics research provides novel insights into determining the clonal relationship between multiple tumours within the same thyroid gland. For eight MPTC patients, we performed whole-exome sequencing and targeted region sequencing to identify the non-synonymous point mutations and gene rearrangements of distinct and spatially separated tumour foci. Among these eight MPTCs, completely discordant mutational spectra were observed in the distinct cancerous nodules of patients MPTC1 and 5, suggesting that these nodules originated from independent precursors. In another three cases (MPTC2, 6, and 8), the distinct MPTC foci of these patients had no other shared mutations except BRAF V600E, also indicating likely independent origins. Two patients (MPTC3 and 4) shared almost identical mutational spectra amongst their separate tumour nodules, suggesting a common clonal origin. MPTC patient 7 had seven cancer foci, of which two foci shared 66.7% of mutations, while the remaining cancer foci displayed no common non-synonymous mutations, indicating that MPTC7 has multiple independent origins accompanied by intraglandular disease dissemination. In this study, we found that 75% of MPTC cases arose as independent tumours, which supports the field cancerization hypothesis describing multiple malignant lesions. MPTC may also arise from intrathyroidal metastases from a single malignant clone, as well as multiple independent origins accompanied by intrathyroidal metastasis.
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http://dx.doi.org/10.1002/path.4696DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706659PMC
May 2016

Erratum: The Enhanced Anticoagulation for Graphene Induced by COOH+ ion Implantation.

Nanoscale Res Lett 2015 12 4;10(1):1014. Epub 2015 Sep 4.

Energy & Materials Engineering Centre, College of Physics and Materials Science, Tianjin Normal University, Tianjin, 300387, China.

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http://dx.doi.org/10.1186/s11671-015-1014-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559551PMC
December 2015

Simultaneous determination of alkaloids and their related tobacco-specific nitrosamines in tobacco leaves using LC-MS-MS.

J Chromatogr Sci 2015 Nov-Dec;53(10):1730-6. Epub 2015 Jun 26.

Yuxi Normal University, Yuxi, P.R. China.

Tobacco alkaloids (e.g., nicotine) and their metabolized tobacco-specific nitrosamines (TSNAs) are very important compounds for tobacco quality and safety. A simple and specific liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of eight tobacco alkaloids and their related four TSNAs in tobacco leaves. The milled tobacco was extracted using 0.1 mol/L ammonium acetate solution and purified using methanol. Mass spectrometry parameters including declustering potential and collision energy were optimized to ensure that both the TSNAs and the tobacco alkaloids have suitable responses. Recoveries for accuracy were in the range of 80.2-105.2%. Intra-day and inter-day repeatability were in the range of 1.7-12.1% and 6.4-18.7%, respectively. Limit of detection and limit of quantitation were estimated in the range of 6 ng/g-45 μg/g and 24 ng/g-90 μg/g, respectively. The established method was applied to investigate the distribution of tobacco alkaloids and TSNAs in four kinds of tobacco. The result showed that the burley and the flue-cured have the highest (0.00047%) and the lowest (0.000024%) percentage of transformation from alkaloids to TSNAs, respectively. Thus, this method can be used for a wide range of samples.
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http://dx.doi.org/10.1093/chromsci/bmv082DOI Listing
June 2016

Synthesis of Ag ion-implanted TiO2 thin films for antibacterial application and photocatalytic performance.

J Hazard Mater 2015 Dec 14;299:59-66. Epub 2015 May 14.

Key Laboratory of Beam Technology and Material Modification of Ministry of Education, Beijing Normal University, Beijing 100875, China.

TiO2 thin films were deposited by spin coating method. Silver ions were implanted into the films using a Metal Vapor Vacuum Arc implanter. The antibacterial ability of implanted films was tested using Escherichia coli removal under fluorescent irradiation and in the dark. The concentration of E. coli was evaluated by plating technique. The photocatalytic efficiency of the implanted films was studied by degradation of methyl orange under fluorescent illumination. The surface free energy of the implanted TiO2 films was calculated by contact angle testing. Vitamin C was used as radical scavengers to explore the antibacterial mechanism of the films. The results supported the model that both generation of reactive oxygen species and release of silver ions played critical roles in the toxic effect of implanted films against E. coli. XPS experimental results demonstrated that a portion of the Ag(Ag(3+)) ions were doped into the crystalline lattice of TiO2. As demonstrated by density functional theory calculations, the impurity energy level of subtitutional Ag was responsible for enhanced absorption of visible light. Ag ion-implanted TiO2 films with excellent antibacterial efficiency against bacteria and decomposed ability against organic pollutants could be potent bactericidal surface in moist environment.
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http://dx.doi.org/10.1016/j.jhazmat.2015.05.014DOI Listing
December 2015

The enhanced anticoagulation for graphene induced by COOH(+) ion implantation.

Nanoscale Res Lett 2015 27;10:14. Epub 2015 Jan 27.

Energy & Materials Engineering Centre, College of Physics and Materials Science, Tianjin Normal University, Tianjin, 300387 China.

Graphene may have attractive properties for some biomedical applications, but its potential adverse biological effects, in particular, possible modulation when it comes in contact with blood, require further investigation. Little is known about the influence of exposure to COOH(+)-implanted graphene (COOH(+)/graphene) interacting with red blood cells and platelets. In this paper, COOH(+)/graphene was prepared by modified Hummers' method and implanted by COOH(+) ions. The structure and surface chemical and physical properties of COOH(+)/graphene were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle measurement. Systematic evaluation of anticoagulation, including in vitro platelet adhesion assays and hemolytic assays, proved that COOH(+)/graphene has significant anticoagulation. In addition, at the dose of 5 × 10(17) ions/cm(2), COOH(+)/graphene responded best on platelet adhesion, aggregation, and platelet activation.
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http://dx.doi.org/10.1186/s11671-014-0705-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4312311PMC
April 2015

Sorafenib neoadjuvant therapy in the treatment of high risk renal cell carcinoma.

PLoS One 2015 3;10(2):e0115896. Epub 2015 Feb 3.

Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.

Objective: To evaluate the clinical efficacy of sorafenib as preoperative neoadjuvant therapy in patients with high risk renal cell carcinoma (RCC).

Materials And Methods: Clinical data of 18 patients with high risk RCC who received surgery done successfully after preoperative neoadjuvant therapy with sorafenib in Peking Union Medical College Hospital (PUMCH) from April 2007 to October 2013 have been reviewed and analyzed in this study.

Results: Among the 18 patients there were 13 male and 5 female, with a median age of 54.6 years. The objective response rate (ORR) of the operation on the selected patients is very high (94.4%), including 4 cases (22.2%) of partial response (PR) and 13 cases (72.2%) of stable disease (SD). After preoperative sorafenib treatment, the average tumor size of the 18 patients decreased from 7.8 cm (ranging from 3.6 to 19.2 cm) to 6.2 cm (ranging from 2.4 to 16.8 cm), and the median value of average tumor CT value decreased from 61HU to 52 HU. Among the 5 patients who had IVC tumor thrombi, the grades of tumor thrombi in 2 patients who were grade II before sorafenib treatment became grade I and grade 0 respectively, 2 patients of grade III both became grade II.

Conclusion: Preoperative neoadjuvant therapy with sorafenib for high risk RCC patients can significantly decrease primary tumor volume as well as tumor thrombus, which could help the nephron-sparing surgery (NSS) or radical nephrectomy to be done successfully.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0115896PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4315594PMC
January 2016

Liquid chromatography with mass spectrometry method based two-step precursor ion scanning for the structural elucidation of flavonoids.

J Sep Sci 2014 Nov 25;37(21):3067-73. Epub 2014 Sep 25.

Yunnan Academy of Tobacco Agricultural Sciences, Yuxi, P.R. China.

Plant flavonoids are very important secondary metabolites for insect and virus control of their host plant and are potent nutrients for humans. To be able to understand the bioavailability and functions of plant flavonoids, it is necessary to reveal their exact chemical structures. Liquid chromatography with tandem mass spectrometry is a powerful approach for structural elucidation of metabolites. In this report, a two-step precursor ion scanning based liquid chromatography with tandem mass spectrometry method was developed for the structural elucidation of plant flavonoids. The established method consists of the two-step precursor ions scanning for possible flavonoids extraction, MS(2) fragment spectra acquisition and comparison with an online database, liquid chromatography retention rules correction, and commercial standards verification. The developed method was used for the structure elucidation of flavonoids in flowers and leaves of tobacco (Nicotiana tabacum), and 17 flavonoids were identified in the tobacco variety Yunyan 97. Nine of the 17 identified flavonoids were considered to be found in tobacco flowers or/and leaves for the first time based on the available references. This method was proved to be very effective and can be used for the identification of flavonoids in other plants.
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http://dx.doi.org/10.1002/jssc.201400720DOI Listing
November 2014

Patchy nanoparticles self-assembled from linear triblock copolymers under spherical confinement: a simulated annealing study.

Soft Matter 2014 Sep;10(35):6831-43

Department of Physics and Material Science, Tianjin Normal University, Tianjin, 300387, China.

The self-assembly of linear ABC triblock copolymers confined in spherical nanopores is studied using a simulated annealing technique. Morphological phase diagrams as a function of the pore diameter, the selectivity of the pore-wall to the terminal blocks, and the copolymer composition are constructed. A variety of patchy nanoparticles and multiple morphological transitions are identified. Janus nanoparticles, which can be regarded as particles with one patch, are observed inside small nanopores. With increasing the pore diameter, the number of patches on a nanoparticle surface increases from one to two, four, five, six, and seven. The size of each patch increases periodically. The number of patches also increases with increasing the wall selectivity. The distribution of the patches on the surface of a given particle is highly symmetric. The interior structures of the patchy nanoparticles and the morphological transition are investigated by calculating the bridging fraction, the mean square end-to-end distance and the average contact number between different components. A series of entropy-driven morphological transitions is predicted. Furthermore, it is found that the overall patchy morphology is largely controlled by the volume fraction of the middle B-block, while the internal structure is largely controlled by the volume fraction ratio of the two terminal blocks. Our study demonstrates that the size of nanopores, the pore-wall selectivity, and the copolymer composition could be utilized as effective means to tune the structure and properties of the anisotropic nanoparticles.
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http://dx.doi.org/10.1039/c4sm00967cDOI Listing
September 2014

Effect of nitrogen atomic percentage on N+-bombarded MWCNTs in cytocompatibility and hemocompatibility.

Nanoscale Res Lett 2014 Mar 25;9(1):142. Epub 2014 Mar 25.

College of Physics and Materials Science, Tianjin Normal University, Tianjin 300387, China.

N+-bombarded multi-walled carbon nanotubes (N+-bombarded MWCNTs), with different nitrogen atomic percentages, were achieved by different N ion beam currents using ion beam-assisted deposition (IBAD) on MWCNTs synthesized by chemical vapor deposition (CVD). Characterizations of N+-bombarded MWCNTs were evaluated by X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), Raman spectroscopy, and contact angle. For comparison, the in vitro cytocompatibility of the N+-bombarded MWCNTs with different N atomic percentages was assessed by cellular adhesion investigation using human endothelial cells (EAHY926) and mouse fibroblast cells (L929), respectively. The results showed that the presence of nitrogen in MWCNTs accelerated cell growth and proliferation of cell culture. The higher nitrogen content of N+-bombarded MWCNTs, the better cytocompatibility. In addition, N+-bombarded MWCNTs with higher N atomic percentage displayed lower platelet adhesion rate. No hemolysis can be observed on the surfaces. These results proved that higher N atomic percentage led N+-bombarded MWCNTs to better hemocompatibility.
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http://dx.doi.org/10.1186/1556-276X-9-142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3987098PMC
March 2014

[One case report of Kimura disease combined with secretory otitis media].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2013 May;27(10):496-7

The patient is a 75 years old man complaining of ear plug and hearing loss for about three days. Physical examination shows liquid in two tympanic cavities. Chronic congestion occurs to nasal mucosa. Bilateral inferior turbinates are a little bigger than normal. Both inferior meatus are unblocked and pharyngeal recess is smooth. This patient is fat. Bilateral eye lids are swollen. A tumour could be touched in bilateral parotids with good movement and no tenderness. Its size is as big as an egg. Several lymph nodes could be touched under the jaw. The blood test is normal and IgG, IgA, IgM, ANA, anti-ENA, C3, C4, CRP and ESR is normal but RF is high. The function of liver and kidney is normal. B-mode ultrasonography result shows bilateral parotids are diffusely swollen and several swollen lymph nodes in cervix. Pathology examination of lower mandibular lymph node and parotid shows follicular lymphaticus hyperplasia with several plasmacytes and eosinophil granulocytes. The diagnosis accords with Kimura disease.
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May 2013

[Investigation on the antigens responsible for allergic rhinitis occurred in the population of Kunshan district].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2012 Apr;26(8):370-1

Department of Otolaryngology, the First People' Hospital of Kunshan, Kunshan, 215300, China.

Objective: To investigate the distribution and descriptions of the common antigens of allergic rhinitis occurred in the population of Kunshan district so as to provide evidence for clinical diagnosis,treatment and prevention.

Method: Eight hundred and twenty allergic rhinitis patients were examined skin prick test (SPT) with 18 kinds of antigen extract of allergic rhinitis.

Result: Six hundred and ninety-four (84.6%) cases were detected at least one antigen, 581 (83.7%) cases were detected two or more antigens. The most common antigens among all the positive cases of skin prick tests were dust mite 492 (70.9%), house dust mite 473 (68.2%), crab 32 (4.5%) and egg white 26 (3.7%).

Conclusion: Mite, crab and egg white are the most common antigens of allergic rhinitis in Kunshan. SPT is important for the diagnosis, treatment and prevention.
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April 2012

Accelerated solvent extraction for GC-based tobacco fingerprinting and its comparison with simultaneous distillation and extraction.

Talanta 2010 Apr 11;81(1-2):650-6. Epub 2010 Jan 11.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.

An accelerated solvent extraction (ASE) procedure has been developed as a pretreatment method for chemical fingerprinting of volatile and semi-volatile components in cut tobacco. The ASE extraction conditions including temperature, operation pressure and extraction cycles were optimized to maximize extraction yield. The method was validated with repeatability, recovery and linearity. Compared with simultaneous distillation extraction (SDE), ASE provides higher extraction yields, less extraction time, lower solvent consumption and less labor time, and is more suitable for tobacco sample preparation. A typical ASE extract was analyzed by gas chromatography/time-of-flight mass spectrometry (GC-TOFMS). A total of 305 components with signal-to-noise ratio higher than 100 were tentatively identified by NIST05 and Wiley database. Finally, 36 cigarette samples from six cigarette brands were analyzed using the developed chemical fingerprinting method. Partial least squares-discriminant analysis shows good discrimination of different cigarette brands. The results indicate that ASE method can serve as high-throughput sample preparation technique for cigarette chemical fingerprint analysis.
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http://dx.doi.org/10.1016/j.talanta.2009.12.054DOI Listing
April 2010
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