Publications by authors named "Jianguo Zhang"

725 Publications

Clinicopathological features and genomic profiles of hepatoid adenocarcinoma of the lung: Report of four cases.

Pathol Res Pract 2021 Nov 20;229:153652. Epub 2021 Nov 20.

Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, PR China. Electronic address:

Objective: Pathological features of hepatoid adenocarcinoma of the lung (HAL) are similar to those of hepatocellular carcinoma (HCC) and HAL has a poor prognosis. In this study, we aimed to elucidate clinicopathologic and molecular features of HAL.

Methods: Four cases of HAL patients with one lobe of the lung resected were enrolled into the study. Next generation sequencing (NGS) of a 425-gene panel was performed on tumor tissue samples.

Results: The most frequently mutated gene was TP53 in three cases of primary HAL and one case of metastatic HAL, with a mutation rate of 100%. Also, CDK8, CDKN2A, EPHA5, SMARCA4, and STK11 were detected as high-frequency mutations, with a mutation rate of 50%. The types of TP53 mutation included two missense variants and two frameshift ones. The TP53 mutation was related to the occurrence of HAL.

Conclusion: HAL could be caused by genetic mutations and is closely related to TP53 mutation.
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http://dx.doi.org/10.1016/j.prp.2021.153652DOI Listing
November 2021

A four-band and polarization-independent BDS-based tunable absorber with high refractive index sensitivity.

Phys Chem Chem Phys 2021 Nov 25. Epub 2021 Nov 25.

Fujian Provincial Key Laboratory for Advanced Micro-Nano Photonics Technology and Devices, Quanzhou Normal University, Quanzhou 362000, China.

A four-band terahertz tunable narrow-band perfect absorber based on a bulk Dirac semi-metallic (BDS) metamaterial with a microstructure is designed. The three-layer structure of this absorber from top to bottom is the Dirac semi-metallic layer, the dielectric layer and the metal reflector layer. Based on the Finite Element Method (FEM), we use the simulation software CST STUDIO SUITE to simulate the absorption characteristics of the designed absorber. The simulation results show that the absorption rate of the absorber is over 93% at frequencies of 1.22, 1.822, 2.148 and 2.476 THz, and three of them have achieved a perfect absorption rate of more than 95%. We use the localized surface plasmon resonance (LSPR), impedance matching and other theories to analyze its physical mechanism in detail. The influence of the geometric structure parameters of the absorber and the incident angle of electromagnetic waves on the absorption performance has also been studied in detail. Due to the rotational symmetry of the structure, the designed absorber has excellent polarization insensitivity. In addition, the maximum adjustable range of absorption frequency is 0.051 THz, which can be achieved by changing the Fermi energy of BDS. We also define the refractive index sensitivity (), which is 39.1, 75.4, 119.1 and 122.0 GHz RIU for the four absorption modes when the refractive index varies in the range of 1 to 1.9. This high-performance absorber has a very good development prospect in the frontier fields of bio-chemical sensing and special environmental detection.
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http://dx.doi.org/10.1039/d1cp04568gDOI Listing
November 2021

Temporal order of signal propagation within and across intrinsic brain networks.

Proc Natl Acad Sci U S A 2021 Nov;118(48)

Department of Neurology & Neurological Sciences, Stanford University, Stanford, CA 94305;

We studied the temporal dynamics of activity within and across functional MRI (fMRI)-derived nodes of intrinsic resting-state networks of the human brain using intracranial electroencephalography (iEEG) and repeated single-pulse electrical stimulation (SPES) in neurosurgical subjects implanted with intracranial electrodes. We stimulated and recorded from 2,133 and 2,372 sites, respectively, in 29 subjects. We found that N1 and N2 segments of the evoked responses are associated with intra- and internetwork communications, respectively. In a separate cognitive experiment, evoked electrophysiological responses to visual target stimuli occurred with less temporal separation across pairs of electrodes that were located within the same fMRI-defined resting-state networks compared with those located across different resting-state networks. Our results suggest intranetwork prior to internetwork information processing at the subsecond timescale.
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http://dx.doi.org/10.1073/pnas.2105031118DOI Listing
November 2021

Real-time removal of stimulation artifacts in closed-loop deep brain stimulation.

J Neural Eng 2021 Nov 24. Epub 2021 Nov 24.

Institute of Science and Technology for Brain-inspired Intelligence, Fudan University, 220 Handan Road, Yangpu District, Shanghai, 200433, CHINA.

Objective: Closed-loop deep brain stimulation (DBS) with neural feedback has shown great potential in improving the therapeutic effect and reducing side effects. However, the amplitude of stimulation artifacts is much larger than the local field potentials, which remains a bottleneck in developing a closed-loop stimulation strategy with varied parameters.

Approach: We proposed an irregular sampling method for the real-time removal of stimulation artifacts. The artifact peaks were detected by applying a threshold to the raw recordings, and the samples within the contaminated period of the stimulation pulses were excluded and replaced with the interpolation of the samples prior to and after the stimulation artifact duration. This method was evaluated with both simulation signals and in vivo closed-loop DBS applications in Parkinsonian animal models.

Main Results: The irregular sampling method was able to remove the stimulation artifacts effectively with the simulation signals. The relative errors between the power spectral density of the recovered and true signals within a wide frequency band (2-150 Hz) were 2.14%, 3.93%, 7.22%, 7.97% and 6.25% for stimulation at 20 Hz, 60 Hz, 130 Hz, 180 Hz, and stimulation with variable low and high frequencies, respectively. This stimulation artifact removal method was verified in real-time closed-loop DBS application in vivo, and the artifacts were effectively removed during stimulation with frequency continuously changing from 130 Hz to 1 Hz and stimulation adaptive to beta oscillations.

Significance: The proposed method provides an approach for real-time removal in closed-loop DBS applications, which is effective in stimulation with low frequency, high frequency, and variable frequency. This method can facilitate the development of more advanced closed-loop DBS strategies.
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http://dx.doi.org/10.1088/1741-2552/ac3cc5DOI Listing
November 2021

Unique features of the mA methylome and its response to drought stress in sea buckthorn ( Linn.).

RNA Biol 2021 Nov 21:1-10. Epub 2021 Nov 21.

State Key Laboratory of Tree Genetics and Breeding & Key Laboratory of Tree Breeding and Cultivation, National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.

In plants, recent studies have revealed that N6-methyladenosine (mA) methylation of mRNA has potential regulatory functions of this mRNA modification in many biological processes. mA methyltransferase, mA demethylase and mA-binding proteins can cause differential phenotypes, indicating that mA may have critical roles in the plant. In this study, we depicted the mA map of sea buckthorn ( Linn.) transcriptome. Similar to , mA sites of sea buckthorn transcriptome is significantly enriched around the stop codon and within 3'-untranslated regions (3'UTR). Gene ontology analysis shows that the mA modification genes are associated with metabolic biosynthesis. In addition, we identified 13,287 different mA peaks (DMPs) between leaf under drought (TR) and control (CK) treatment. It reveals that mA has a high level of conservation and has a positive correlation with mRNA abundance in plants. GO and KEGG enrichment results showed that DMP modification DEGs in TR were particularly associated with ABA biosynthesis. Interestingly, our results showed three mA demethylase ( and ) genes were significantly increased following drought stress, which indicated that it may contributed the decreased mA levels. This exhaustive mA map provides a basis and resource for the further functional study of mRNA mA modification in abiotic stress.
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http://dx.doi.org/10.1080/15476286.2021.1992996DOI Listing
November 2021

Prognostic model of lung adenocarcinoma constructed by the CENPA complex genes is closely related to immune infiltration.

Pathol Res Pract 2021 Dec 3;228:153680. Epub 2021 Nov 3.

Department of Pathology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China; Medical School of Nantong University, Nantong 226001, Jiangsu, China. Electronic address:

Background: Lung adenocarcinoma (LUAD) is still one of the primary malignant diseases leading to higher mortality worldwide. It has been previously reported that multiple genes in the CENPA-nucleosome associated complex (NAC) complex in lung cancer can be used as prognostic markers; however, there is lack of comprehensive research on the CENPA-NAC complex.

Methods: The hub genes of lung cancer were obtained by analyzing multiple gene expression omnibus (GEO) lung cancer datasets. The key genes of the CENPA-NAC complex in the evolution of LUAD were identified according to lung cancer data obtained from The Cancer Genome Atlas (TCGA) database, and the key genes were constructed as a survival prognostic model. The relationship between the model and immune cell infiltration was studied by the Tumor Immune Estimation Resource (TIMER) and single-sample gene set enrichment analysis (ssGSEA) studies.Droplet Digital polymerase chain reaction (ddPCR) was used to verify the effectiveness of the prognostic model to predict survival using clinical samples.

Results: A comprehensive study showed that CENPA, CENPH, CENPM, CENPN and CENPU were key genes in the development and evolution of LUAD. The constructed survival prognosis model was an independent risk factor for LUAD and can be used to assess the survival of LUAD patients. The risk score was closely related to the infiltration of multiple immune cells. The independent cohorts GSE31210 and GSE50081 further confirmed the validity of the prognostic model, and finally, the model was validated with clinical samples.

Conclusions: In conclusion, the results of the present study showed that CENPA, CENPH, CENPM, CENPN, and CENPU are a group of potential prognostic markers in LUAD. The constructed model has been confirmed to be applicable in the clinical setting in evaluating the survival of patients with LUAD, and providing more evidence on immunotherapy for LUAD.
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http://dx.doi.org/10.1016/j.prp.2021.153680DOI Listing
December 2021

Comprehensive study of a novel immune-related lncRNA for prognosis and drug treatment of cervical squamous cell carcinoma.

Am J Transl Res 2021 15;13(10):11771-11785. Epub 2021 Oct 15.

Department of Oncology, The Third People's Hospital of Nantong Nantong 226001, Jiangsu, China.

A comprehensive study focusing on immune-related long non-coding RNAs (lncRNAs) in cervical cancer (CC) was performed. Through the integration of TCGA data, a total of 266 immune-related lncRNAs were obtained. We defined all samples as an entire set, and randomly divided them into train set and test set at a ratio of 1:1. Univariate, LASSO and multivariate Cox regression analyses were carried out based on train set for key lncRNAs (UBL7-AS1, AC083809.1, LIPE-AS1, PCED1B-AS1, ELFN1-AS1 and NCK1-DT) to construct a prognostic model, while the others were used for validation. The overall survival (OS) suggested that we may have longer survival expectations for patients classified into the low-risk group. The values of risk score in univariate analysis and multivariate analysis were all less than 0.05, indicating the ability of risk score to independently assess the prognosis of patients. For clinical application, a nomogram with a high degree of agreement between the predicted curve and the actual curve was constructed. Subsequently, immune status and chemotherapy response were investigated in two prognostic subtypes. The associations between risk score and immune cell were estimated, in which CD8+ T cells showed the highest positive correlation and activated mast cell showed the highest negative correlation. In addition, checkpoint proteins (CTLA4, LAG3, PD-1, and TIGIT) showing negative correlation with risk score were found to be upregulated in low-risk group. A total of 3 chemotherapy drugs including paclitaxel, vinorelbine and methotrexate were considered effective in patients of high-risk group. Using 6 key immune-related lncRNAs, we identified two prognostic subtypes and provided new insights for CC immunotherapy.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581925PMC
October 2021

Expert consensus on the clinical application of recombinant adenovirus human p53 for head and neck cancers.

Int J Oral Sci 2021 Nov 16;13(1):38. Epub 2021 Nov 16.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Head and Neck Oncology, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

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http://dx.doi.org/10.1038/s41368-021-00145-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8595718PMC
November 2021

4,5-Dicyano-1,2,3-Triazole-A Promising Precursor for a New Family of Energetic Compounds and Its Nitrogen-Rich Derivatives: Synthesis and Crystal Structures.

Molecules 2021 Nov 7;26(21). Epub 2021 Nov 7.

Chemical Engineering Department, Mendeleev University of Chemical Technology, 9 Miusskaya Sq., 125047 Moscow, Russia.

The nitrogen-rich compounds and intermediates with structure of monocyclic, bicyclic, and fused rings based on 1,2,3-triazole were synthesized and prepared by using a promising precursor named 4,5-dicyano-1,2,3-triazole, which was obtained by the cyclization reaction of diaminomaleonitrile. Their structure and configurational integrity were assessed by Fourier transform-infrared spectroscopy (FT-IR), mass spectrometry (MS), and elemental analysis (EA). Additionally, fourteen compounds were further confirmed by X-ray single crystal diffraction. Meanwhile, the physical properties of four selected compounds (·HO, ·HO, ·HO, and ) including thermal stability, detonation parameters, and sensitivity were also estimated. All these compounds could be considered to construct more abundant 1,2,3-triazole-based neutral energetic molecules, salts, and complex compounds, which need to continue study in the future in the field of energetic materials.
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http://dx.doi.org/10.3390/molecules26216735DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8588547PMC
November 2021

Energetic and magnetic directional aggregation properties of [email protected] particles prepared via a microcrystalline co-precipitation route.

Nanotechnology 2021 Nov 29;33(8). Epub 2021 Nov 29.

State Key Laboratory of Explosion Science and Technology, Beijing Institute of Technology, Beijing 100081, People's Republic of China.

The development of new electromagnetic interference materials has attracted much attention in the information warfare. Herein, a novel [email protected] particle was synthesized via a microcrystalline co-precipitation method. X-ray diffractions, scanning electron microscopes and vibrating sample magnetometer measurements were used to characterize the products. The results indicated that the surface of the potassium picrate (KPA) crystals was covered by magneticFe3O4nanoparticles, and composite particles exhibited excellent magnetic properties. Furthermore, the thermal behavior of the composite particles was investigated by differential scanning calorimetry, which showed that the composite particles inherited the energetic property of pure KPA crystals when the mass fraction of magnetic component was 50%, or 65%. As for the composite particles with 75% magnetic component, the thermal stability of was poor. In addition, the magnetic directional aggregation performance of composite particles was analyzed by dynamic simulation, which moved toward the magnetic source. For the composite particles with 50% magnetic component, the maximum concentration was about 63 times of the initial concentration, and the peak velocity was 0.63 m s. With the mass fraction of magnetic component increasing to 65%, the concentration and velocity of the composite particles generally increased at the corresponding moment. As the mass fraction of magnetic component increased to 75%, the change of them was not obvious. Therefore, the composite particles withFe3O4/KPA mass ratios of 65/35 had the best comprehensive properties. The excellent energetic and magnetic directional aggregation properties can allow the composites to be used in many potential applications in the information warfare.
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http://dx.doi.org/10.1088/1361-6528/ac38e9DOI Listing
November 2021

Abnormal Functional Brain Network in Parkinson's Disease and the Effect of Acute Deep Brain Stimulation.

Front Neurol 2021 14;12:715455. Epub 2021 Oct 14.

Department of Functional Neurosurgery, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.

The objective of this study was to use functional connectivity and graphic indicators to investigate the abnormal brain network topological characteristics caused by Parkinson's disease (PD) and the effect of acute deep brain stimulation (DBS) on those characteristics in patients with PD. We recorded high-density EEG (256 channels) data from 21 healthy controls (HC) and 20 patients with PD who were in the DBS-OFF state and DBS-ON state during the resting state with eyes closed. A high-density EEG source connectivity method was used to identify functional brain networks. Power spectral density (PSD) analysis was compared between the groups. Functional connectivity was calculated for 68 brain regions in the theta (4-8 Hz), alpha (8-13 Hz), beta1 (13-20 Hz), and beta2 (20-30 Hz) frequency bands. Network estimates were measured at both the global (network topology) and local (inter-regional connection) levels. Compared with HC, PSD was significantly increased in the theta ( = 0.003) frequency band and was decreased in the beta1 ( = 0.009) and beta2 ( = 0.04) frequency bands in patients with PD. However, there were no differences in any frequency bands between patients with PD with DBS-OFF and DBS-ON. The clustering coefficient and local efficiency of patients with PD showed a significant decrease in the alpha, beta1, and beta2 frequency bands ( < 0.001). In addition, edgewise statistics showed a significant difference between the HC and patients with PD in all analyzed frequency bands ( < 0.005). However, there were no significant differences between the DBS-OFF state and DBS-ON state in the brain network, except for the functional connectivity in the beta2 frequency band ( < 0.05). Compared with HC, patients with PD showed the following characteristics: slowed EEG background activity, decreased clustering coefficient and local efficiency of the brain network, as well as both increased and decreased functional connectivity between different brain areas. Acute DBS induces a local response of the brain network in patients with PD, mainly showing decreased functional connectivity in a few brain regions in the beta2 frequency band.
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http://dx.doi.org/10.3389/fneur.2021.715455DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551554PMC
October 2021

FAT1 and PTPN14 Regulate the Malignant Progression and Chemotherapy Resistance of Esophageal Cancer through the Hippo Signaling Pathway.

Anal Cell Pathol (Amst) 2021 19;2021:9290372. Epub 2021 Oct 19.

Department of Medical Oncology, The Second People's Hospital of Lianyungang (The Oncology Hospital of Lianyungang), No 161, XinFu Road, Lianyungang, Jiangsu 222000, China.

Background: Esophageal cancer (EC) is a common malignant tumor, which brings heavy economic burden to patients and society. Therefore, it is important to understand the molecular mechanism of recurrence, metastasis, and drug resistance of esophageal cancer.

Methods: Human esophageal cancer cell line TE13 (poorly differentiated squamous cell carcinoma) and normal human esophageal epithelial cell line het-1a were selected for aseptic culture. At the same time, 6 bottles of TE13 cell line were inoculated in logarithmic phase. Cell apoptosis was analyzed by flow cytometry (FCM). Cell clone formation assay was used to analyze the proliferation. Fibronectin-coated dishes were used to detect the characteristics of cell adhesion to extracellular matrix. The Transwell method was used to detect the cell invasion ability. Western blot was used to analyze the expression of Yap1, PTPN14, FAT1, and Myc.

Results: Results showed that FAT1 and PTPN14 were downregulated, while Yap1 was upregulated in esophageal cancer tissues. FAT1 inhibited the proliferation, adhesion, and invasion of human esophageal cancer cell lines, which might be associated with the upregulation of PTPN14 and the inhibition of Yap1 and Myc.

Conclusion: The results suggested that PTPN14 and FAT1 could regulate malignant progression and chemotherapy resistance of esophageal cancer based on the Hippo signaling pathway.
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http://dx.doi.org/10.1155/2021/9290372DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548181PMC
October 2021

Testicular blood supply and growth in children with high cryptorchidism treated with gubernaculum preserving vs. cutting laparoscopic orchiopexy: A pilot trial.

J Pediatr Urol 2021 Oct 12. Epub 2021 Oct 12.

Pediatric Surgery, Inner Mongolia Maternal and Child Health Care Hospital, Huhehaote, Inner Mongolia, 010020, China. Electronic address:

Introduction: It is still controversial whether the frenum of testis should be retained in laparoscopic testicular fixation.

Materials And Methods: It is a prospective experiment to study testicular preservation on testicular growth in children with high cryptorchidism.

Results: From January 2018 to June 2020, 120 children with high cryptorchidism in Inner Mongolia Maternal and Child Health Care Hospital and The Fourth Hospital of Baotou were randomly divided into retention group (60 cases in group P, aged 1-3 years, average 1.85 ± 0.58) and cutting group (60 cases in group C, aged 1-3 years, average 1.75 ± 0.66) (P = 0.52). PSV, EDV, RI and testicular volume were measured by color Doppler ultrasound before operation and 1,3,6,12 months after operation. No, there was significant difference between the two groups (P > 0.05).

Conclusions: There is no significant difference in testicular blood supply and growth between laparoscopic testicular fixation and frenulum amputation in children with high cryptorchidism.
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http://dx.doi.org/10.1016/j.jpurol.2021.10.002DOI Listing
October 2021

Disruptive Variants Implicated in Congenital Vertebral Malformations.

Genes (Basel) 2021 Oct 14;12(10). Epub 2021 Oct 14.

Department of Orthopedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China.

Genetic perturbations in nicotinamide adenine dinucleotide de novo (NAD) synthesis pathway predispose individuals to congenital birth defects. The encodes the final enzyme in the de novo NAD synthesis pathway and, therefore, plays an important role in NAD metabolism and organ embryogenesis. Biallelic mutations in the gene have been reported to be causative of congenital organ defects known as VCRL syndrome (Vertebral-Cardiac-Renal-Limb syndrome). Here, we analyzed the genetic variants in in an exome-sequenced cohort consisting of patients with congenital vertebral malformations (CVMs). A total number of eight variants in , including two truncating variants and six missense variants, were identified in nine unrelated patients. All enrolled patients presented multiple organ defects, with the involvement of either the heart, kidney, limbs, or liver, as well as intraspinal deformities. An in vitro assay using COS-7 cells demonstrated either significantly reduced protein levels or disrupted enzymatic activity of the identified variants. Our findings demonstrated that functional variants in were involved in the complex genetic etiology of CVMs and provided further evidence for the causative variants in congenital NAD Deficiency Disorder.
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http://dx.doi.org/10.3390/genes12101615DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535205PMC
October 2021

Simultaneous Realization of Superoleophobicity and Strong Substrate Adhesion in Water via a Unique Segment Orientation Mechanism.

Adv Mater 2021 Oct 22:e2106908. Epub 2021 Oct 22.

Charles Institute of Dermatology, School of Medicine, University College Dublin, Dublin 4, Ireland.

The underwater superoleophobicity of a coating is often caused by its preferential water affinity, which, however, normally weakens the substrate adhesion property. In this work, a new strategy is reported for achieving strong underwater adhesion between a well-designed amphiphilic polyurethane coating and a diverse range of substrates while also rendering the coating surface's superoleophobicity. When the coating, which is a mixture of an amphiphilic polyurethane and a water miscible solvent, is immersed in water, the hydrophobic segments aggregate to orientate and pile along the surface of substrates via a segment orientation mechanism triggered by solvent exchange with water penetration to exert strong adhesion. At the same time, the hydrophilic segments will physically crosslink to form a hydrogel coating, endowing the substrate with underwater superoleophobicity. This work provides a facile, versatile, and scalable approach for the future design of superoleophobic coatings in a water environment.
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http://dx.doi.org/10.1002/adma.202106908DOI Listing
October 2021

Comparison of cognitive performance between patients with Parkinson's disease and dystonia using an intraoperative recognition memory test.

Sci Rep 2021 Oct 20;11(1):20724. Epub 2021 Oct 20.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Neuroscientific studies on the function of the basal ganglia often examine the behavioral performance of patients with movement disorders, such as Parkinson's disease (PD) and dystonia (DT), while simultaneously examining the underlying electrophysiological activity during deep brain stimulation surgery. Nevertheless, to date, there have been no studies comparing the cognitive performance of PD and DT patients during surgery. In this study, we assessed the memory function of PD and DT patients with the Montreal Cognitive Assessment (MoCA) and the Mini-Mental State Examination (MMSE). We also tested their cognitive performance during the surgery using a continuous recognition memory test. The results of the MoCA and MMSE failed to reveal significant differences between the PD and DT patients. Additionally, no significant difference was detected by the intraoperative memory test between the PD and DT patients. The intraoperative memory test scores were highly correlated with the MMSE scores and MoCA scores. Our data suggest that DT patients perform similarly to PD patients in cognitive tests during surgery, and intraoperative memory tests can be used as a quick memory assessment tool during surgery.
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http://dx.doi.org/10.1038/s41598-021-99317-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528828PMC
October 2021

The amplitude of low-frequency fluctuation predicts levodopa treatment response in patients with Parkinson's disease.

Parkinsonism Relat Disord 2021 Nov 6;92:26-32. Epub 2021 Oct 6.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China; Stereotactic and Functional Neurosurgery Laboratory, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China; Beijing Key Laboratory of Neurostimulation, Beijing, China. Electronic address:

Introduction: Levodopa has become the main therapy for motor symptoms of Parkinson's disease (PD). This study aimed to test whether the amplitude of low-frequency fluctuation (ALFF) computed by fMRI could predict individual patient's response to levodopa treatment.

Methods: We included 40 patients. Treatment efficacy was defined based on motor symptoms improvement from the state of medication off to medication on, as assessed by the Unified Parkinson's Disease Rating Scale score III. Two machine learning models were constructed to test the prediction ability of ALFF. First, the ensemble method was implemented to predict individual treatment responses. Second, the categorical boosting (CatBoost) classification was used to predict individual levodopa responses in patients classified as moderate and superior responders, according to the 50% threshold of improvement. The age, disease duration and treatment dose were controlled as covariates.

Results: No significant difference in clinical data were observed between moderate and superior responders. Using the ensemble method, the regression model showed a significant correlation between the predicted and the observed motor symptoms improvement (r = 0.61, p < 0.01, mean absolute error = 0.11 ± 0.02), measured as a continuous variable. The use of the Catboost algorithm revealed that ALFF was able to differentiate between moderate and superior responders (area under the curve = 0.90). The mainly contributed regions for both models included the bilateral primary motor cortex, the occipital cortex, the cerebellum, and the basal ganglia.

Conclusion: Both continuous and binary ALFF values have the potential to serve as promising predictive markers of dopaminergic therapy response in patients with PD.
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http://dx.doi.org/10.1016/j.parkreldis.2021.10.003DOI Listing
November 2021

Transient tracheal stenosis due to trachea compression and stretching after spinal deformity correction surgery.

J Clin Anesth 2021 Dec 9;75:110542. Epub 2021 Oct 9.

Department of Orthopedics, Peking Union Medical College Hospital, Beijing, PR China. Electronic address:

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http://dx.doi.org/10.1016/j.jclinane.2021.110542DOI Listing
December 2021

Microstimulation Is a Promising Approach in Achieving Better Lead Placement in Subthalamic Nucleus Deep Brain Stimulation Surgery.

Front Neurol 2021 22;12:683532. Epub 2021 Sep 22.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

The successful application of subthalamic nucleus (STN) deep brain stimulation (DBS) surgery relies mostly on optimal lead placement, whereas the major challenge is how to precisely localize STN. Microstimulation, which can induce differentiating inhibitory responses between STN and substantia nigra pars reticulata (SNr) near the ventral border of STN, has indicated a great potential of breaking through this barrier. This study aims to investigate the feasibility of localizing the boundary between STN and SNr (SSB) using microstimulation and promote better lead placement. We recorded neurophysiological data from 41 patients undergoing STN-DBS surgery with microstimulation in our hospital. Trajectories with typical STN signal were included. Microstimulation was applied near the bottom of STN to determine SSB, which was validated by the imaging reconstruction of DBS leads. In most trajectories with microstimulation (84.4%), neuronal firing in STN could not be inhibited by microstimulation, whereas in SNr long inhibition was observed following microstimulation. The success rate of localizing SSB was significantly higher in trajectories with microstimulation than those without. Moreover, results from imaging reconstruction and intraoperative neurological assessments demonstrated better lead location and higher therapeutic effectiveness in trajectories with microstimulation and accurately identified SSB. Microstimulation on microelectrode recording is an effective approach to localize the SSB. Our data provide clinical evidence that microstimulation can be routinely employed to achieve better lead placement.
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http://dx.doi.org/10.3389/fneur.2021.683532DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8493285PMC
September 2021

Outcomes of Posterior Lumbar Hemivertebra Resection and Short Fusion in Patients With Severe Sacral Tilt.

Neurospine 2021 Sep 30;18(3):562-569. Epub 2021 Sep 30.

Department of Orthopedics, Peking Union Medical College Hospital, Beijing, China.

Objective: To introduce surgical strategies to restore coronal balance during limited fusion for early lumbar hemivertebra resection in patients with severe sacral tilt.

Methods: Sacral tilt was defined as a sacral tilt angle ≥ 5, and severe sacral tilt was defined as a sacral tilt angle > 10. From July 2004 to December 2017, 73 consecutive patients treated with posterior hemivertebra resection and short fusion in our institution were evaluated. Severe sacral tilt was noted in 26 patients (14 boys and 12 girls), and all were enrolled in this study. Undercorrection of the primary lumbar curve as compensation for the sacral tilt and short fusion was performed in these patients. The medical charts and imaging data of the patients were retrospectively reviewed to evaluate the outcomes.

Results: All patients were followed for at least 2 years. The mean age at the time of surgery was 3.7 (2-9) years old, with a total of 31 lumbar hemivertebra excised. On average, 2.8 (2-5) segments were fused for each patient. Sacral tilt minimally improved from 14.5° preoperatively to 13.6° postoperatively (p = 0.15) and remained stable at the follow-up. The overall lumbar curve was 41.9° preoperatively, 11.7° immediately postoperatively, and 14.6° at the final follow-up. The segmental scoliosis curve was 39.1° preoperatively, 9.7° immediately postoperatively, and 11.2° at the final follow-up. Segmental kyphosis was corrected from 27.2° to 6.5° after the surgery and was 7.1° at the latest follow-up.

Conclusion: Sacral tilt is seen in patients with congenital scoliosis in lumbar hemivertebra. Undercorrection of the lumbar curve and segmental scoliosis to compensate for sacral tilt and short fusion after hemivertebra resection may be helpful to restore coronal balance and preserve mobility in segments in patients with pronounced severe sacral tilt.
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http://dx.doi.org/10.14245/ns.2142376.188DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497244PMC
September 2021

miR-141-3p promotes the cisplatin sensitivity of osteosarcoma cell through targeting the Glutaminase (GLS)-mediated glutamine metabolism.

Curr Mol Med 2021 Oct 4. Epub 2021 Oct 4.

Department of Spinal Surgery, Weifang People's Hospital. Weifang City, Shandong Province. China.

Aims: This study aimed to evaluate the roles and molecular targets of miRNA-141-3p in the cisplatin sensitivity of osteosarcoma.

Background: Osteosarcoma is one of the most common-type bone tumors, occurring mainly in children and adolescent. Cancer cells display dysregulated cellular metabolism, such as the abnormally elevated glutamine metabolism.

Objective: Non-coding RNA miRNA-141-3p has been reported to act as a tumor suppressor in osteosarcoma. Currently, the precise molecular mechanisms for the miR-141-3p-mediated chemosensitivity through regulating glutamine metabolism remain unclear.

Methods: We collected thirty-paired OS tumors and their adjacent normal tissues. The osteosarcoma cell lines [Saos-2] and normal osteoblast cells, hFOB1.19 were used for in vitro experiments. RT-qPCR and Western blot were applied for gene expression detections. Targets of miR-141-3p was predicted from starBase. The MTT and flow cytometric assays were performed to determine cell growth and apoptosis rates. The cellular glutamine metabolism was monitored by glutamine uptake assay and the glutaminase [GLS] activity assay.

Results: We report miR-141-3p were significantly downregulated in osteosarcoma tissues and cells. Overexpression of miR-141-3p suppressed OS cell growth and sensitized OS cells to cisplatin. In addition, glutamine metabolism was significantly increased in osteosarcoma. We characterized that GLS played oncogenic roles in osteosarcoma and validated GLS was a direct target of miR-141-3p in OS cells. Specifically, rescue experiments consistently demonstrated the miR-141-3p-promoted cisplatin sensitivity was through direct targeting GLS.

Conclusion: Overall, our findings revealed new molecular mechanisms of the miR-141-3p-modulated cisplatin sensitization through targeting the GLS-glutamine metabolism pathway. This study will contribute to developing new therapeutic approaches for the treatments of chemoresistant osteosarcoma.
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http://dx.doi.org/10.2174/1566524021666211004112055DOI Listing
October 2021

Whole Exome Sequencing Uncovered the Genetic Architecture of Growth Hormone Deficiency Patients.

Front Endocrinol (Lausanne) 2021 13;12:711991. Epub 2021 Sep 13.

Department of Orthopedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.

Purpose: Congenital growth hormone deficiency (GHD) is a rare and etiologically heterogeneous disease. We aim to screen disease-causing mutations of GHD in a relatively sizable cohort and discover underlying mechanisms a candidate gene-based mutational burden analysis.

Methods: We retrospectively analyzed 109 short stature patients associated with hormone deficiency. All patients were classified into two groups: Group I (n=45) with definitive GHD and Group II (n=64) with possible GHD. We analyzed correlation consistency between clinical criteria and molecular findings by whole exome sequencing (WES) in two groups. The patients without a molecular diagnosis (n=90) were compared with 942 in-house controls for the mutational burden of rare mutations in 259 genes biologically related with the GH axis.

Results: In 19 patients with molecular diagnosis, we found 5 possible GHD patients received known molecular diagnosis associated with GHD ( [c.2329T>A, c.7131C>G], [c.731G>A], [c.1102delC], [c.187_207dup]). By mutational burden analysis of predicted deleterious variants in 90 patients without molecular diagnosis, we found that ( = 0.005), ( = 0.006), ( = 0.021) and ( = 0.040) represented top genes enriched in GHD patients.

Conclusion: Our study revealed the discrepancies between the laboratory testing and molecular diagnosis of GHD. These differences should be considered when for an accurate diagnosis of GHD. We also identified four candidate genes that might be associated with GHD.
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http://dx.doi.org/10.3389/fendo.2021.711991DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8475633PMC
September 2021

The Similarity between Species Composition of Vegetation and Soil Seed Bank of Grasslands in Inner Mongolia, China: Implications for the Asymmetric Response to Precipitation.

Plants (Basel) 2021 Sep 13;10(9). Epub 2021 Sep 13.

Institute of Fragile Eco-Environment, Nantong University, Nantong 226007, China.

The asymmetric response of productivity to precipitation was recently proposed as an early warning signal for the shifts in temperate grassland function in China. It was hypothesized that the asymmetry was influenced by the increased growth of the newly emerged seedlings from the soil seed bank. Therefore, the seed density in the soil seed bank and the similarity between species composition of the vegetation and the soil seed bank should be maximized where asymmetry was maximized. However, this knowledge was still limited and unconfirmed. In this study, the desert steppe, typical steppe and the transition zone between them (with the highest asymmetry) were selected for studying the similarity index in both 2018 (dry year) and 2019 (wet year). Plant species composition was monitored in situ using an unmanned aerial vehicle. Soil seed bank samples were collected, and the seed bank density and species composition were then examined and identified in the laboratory. Results showed that: (1) The variation in vegetation species richness between the two years was the highest (41%) in the transition zone ( < 0.05), while it was only 7% and 13% for the desert steppe and typical steppe, respectively. The presence of herbaceous species mainly caused the differences in variation among three grassland types. (2) Seed density was the highest in the transition zone (114 seeds/m and 68 seeds/m in the transient and persistent soil seed bank, respectively) ( < 0.05). Additionally, herbaceous species were the main components of the soil seed bank. (3) The similarity index was the highest in the transition zone ( < 0.05), with 38%/44% and 33%/44% for the transient/persistent soil seed bank in 2018 and 2019, respectively. Our study demonstrated that variation in vegetation species composition was very similar to the composition of the seeds accumulated in the soil seed bank. These results warrant further investigation for the mechanism of asymmetric response of productivity to precipitation.
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http://dx.doi.org/10.3390/plants10091890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467124PMC
September 2021

Screening and Characterization of Two Extracellular Polysaccharide-Producing Bacteria from the Biocrust of the Mu Us Desert.

Molecules 2021 Sep 11;26(18). Epub 2021 Sep 11.

Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, Biomass Energy Center for Arid and Semi-Arid Lands, College of Life Sciences, Northwest A & F University, Yangling, Xianyang 712100, China.

The extracellular polysaccharide (EPS) matrix embedding microbial cells and soil particles plays an important role in the development of biological soil crusts (BSCs), which is widely recognized as beneficial to soil fertility in dryland worldwide. This study examined the EPS-producing bacterial strains YL24-1 and YL24-3 isolated from sandy soil in the Mu Us Desert in Yulin, Shaanxi province, China. The strains YL24-1 and YL24-3 were able to efficiently produce EPS; the levels of EPS were determined to be 257.22 μg/mL and 83.41 μg/mL in cultures grown for 72 h and were identified as and sp., respectively. When the strain YL24-3 was compared to YL28-9 using 16S rRNA gene sequencing, the resemblance was 98.6% and the strain was classified as sp. using physiological and biochemical analysis. Furthermore, strain YL24-3 was also identified as a subspecies of YL28-9 on the basis of DNA-DNA hybridization and polar lipid analysis compared with YL28-9. On the basis of the EPS-related genes of relevant strains in the GenBank, several EPS-related genes were cloned and sequenced in the strain YL24-1, including those potentially involved in EPS synthesis, assembly, transport, and secretion. Given the differences of the strains in EPS production, it is possible that the differences in gene sequences result in variations in the enzyme/protein activities for EPS biosynthesis, assembly, transport, and secretion. The results provide preliminary evidence of various contributions of bacterial strains to the formation of EPS matrix in the Mu Us Desert.
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http://dx.doi.org/10.3390/molecules26185521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466918PMC
September 2021

Molecular subtypes based on ferroptosis-related genes and tumor microenvironment infiltration characterization in lung adenocarcinoma.

Oncoimmunology 2021 11;10(1):1959977. Epub 2021 Sep 11.

Department of Pathology, Affiliated Hospital of Nantong University and Medical School of Nantong University, Nantong, China.

Recently, several molecular subtypes with different prognosis have been found in lung adenocarcinoma (LUAD). However, the characteristics of the ferroptosis molecular subtypes and the associated tumor microenvironment (TME) cell infiltration have not been fully studied in LUAD. Using 1160 lung adenocarcinoma samples, we explored the molecular subtypes mediated by ferroptosis-related genes, along with the associated TME cell infiltration. The ferroptosis score was constructed using the least absolute shrinkage and selection operator regression (LASSO) method to quantify the ferroptosis characteristics of a single tumor. Three different molecular subtypes related to ferroptosis, with different prognoses, were identified in LUAD. Analysis of TME cell infiltration revealed immune heterogeneity among the three subtypes. Cluster A was characterized by immunosuppression and was associated with stromal activation. Cluster C was characterized by a large number of immune cells infiltrating the TME, promoting tumor immune response, and it was significantly enriched in immune activation-related signaling pathways. Relatively less infiltration of immune cells was a feature of cluster B. The ferroptosis score can predict tumor subtype, immunity and prognosis. A low ferroptosis score was characterized by immune activation and good prognosis, as seen in the cluster C subtype. Relative immunosuppression and poor prognosis were the characteristics of a high ferroptosis score, as seen in cluster A and B subtypes. At the same time, the anti-PD-1/L1 immunotherapy cohort demonstrated that a low ferroptosis score was associated with higher efficacy of immunotherapy. The ferroptosis score is a promising biomarker that could be of great significance to determine the prognosis, molecular subtypes, TME cell infiltration characteristics and immunotherapy effects in patients with LUAD.
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http://dx.doi.org/10.1080/2162402X.2021.1959977DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8437492PMC
October 2021

Older fusion-surgery age in congenital scoliosis patients is a risk factor for extended length of stay, more estimated blood loss, longer fused segments and higher medical costs: a retrospective study.

BMC Musculoskelet Disord 2021 Sep 12;22(1):779. Epub 2021 Sep 12.

Departments of Orthopaedic Surgery, Peking Union Medical College Hospital (PUMCH), 1st Shuai Fu Yuan Hutong, Dongcheng District, 100730, Beijing, People's Republic of China.

Background: Contradictory opinions about whether early correction and fusion surgeries should be performed for congenital scoliosis (CS) patients at a young age exist. The objectives of this study were to analyze the association between patient characteristics and fusion-surgery outcomes in CS patients treated with spinal correction and fusion surgeries and to report risk factors for extended length of stay (LOS), more estimated blood loss (EBL), longer fused segments and higher medical costs.

Methods: We analyzed data of 1,207 CS inpatients treated with fusion surgeries in our institute from January 2010 - December 2019. All patients underwent spinal X-ray, CT, MRI, echocardiogram and urogenital ultrasound. We analyzed demographic and clinical information and outcome measures, including LOS, EBL, fused segments and medical costs.

Results: Age at fusion (OR = 1.053; p < 0.001), musculoskeletal defects (OR = 1.670; p = 0.004) and thoracic deformity (OR = 1.519; p = 0.03) were risk factors for extended LOS. Age at fusion (OR = 1.117; p < 0.001), male sex (OR = 1.813; p < 0.001), mixed defects (OR = 1.662; p = 0.027) and failure of formation (OR = 1.718; p = 0.021) were risk factors for more EBL. Age at fusion (OR = 1.213; p < 0.001) was a risk factor for longer fused segments. Age at fusion (OR = 1.091; p < 0.001) and thoracic deformity (OR = 1.853; p = 0.004) were risk factors for higher medical costs.

Conclusions: We found that older age at fusion in CS patients is a risk factor for extended LOS, more EBL, longer fused segments and higher medical costs with the risk increasing by 5-21 % for each year of age. Other identified risk factors include thoracic deformity for extended LOS; longer fused segments, higher medical costs, and musculoskeletal defects for extended LOS; and CS type (FF and MD) and sex (male) for more EBL.
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http://dx.doi.org/10.1186/s12891-021-04650-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436475PMC
September 2021

Epilepsy centers in China: Current status and ways forward.

Epilepsia 2021 Nov 12;62(11):2640-2650. Epub 2021 Sep 12.

Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China.

Objective: China has the largest population of patients with epilepsy worldwide, which imposes a heavy burden on the public and health care systems. Several epidemiological surveys on epilepsy have been performed in China. Although these surveys grossly describe the prevalence and gap in treatment of epilepsy, the status of epilepsy centers is unclear. The number of epilepsy centers has increased substantially in recent decades. Therefore, a nationwide investigation of the scale and distribution, personnel, equipment, and epilepsy care capacity of each epilepsy center is of great value.

Methods: In 2017-2018, a multicenter cross-sectional survey was performed by the Commission on Standardized Development of Epilepsy Centers, China Association Against Epilepsy in 31 provinces, autonomous regions, and municipalities. The survey consisted of 74 questions divided into four sections: (1) overview, (2) personnel, (3) essential equipment and facilities, and (4) epilepsy care service capacity. The questions ranged from January 1, 2016 to December 31, 2016. The data were analyzed using descriptive statistics.

Results: There were 358 epilepsy centers for the 1.38 billion national population in 2016. Three quarters were in the eastern and western regions, and >90% were in tertiary hospitals. There were 9688 doctors engaged in epilepsy care, and 4.8% of doctors and electrophysiological physicians/technicians passed the national test for electroencephalography technical accreditation. A total of 9667 patients underwent resective surgeries in 2016. There were 888 vagus nerve stimulation procedures and 275 deep brain stimulation procedures.

Significance: This study is the first unique survey of epilepsy centers in China. Despite their rapid development, epilepsy centers cannot meet patients' needs at this stage. The results provide data-based evidence for the formulation of policies related to epilepsy service planning.
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http://dx.doi.org/10.1111/epi.17058DOI Listing
November 2021

The Mediating Role of Loneliness Between Social Support and Depressive Symptoms Among Chinese Rural Adolescents During COVID-19 Outbreak: A Comparative Study Between Left-Behind and Non-left-behind Students.

Front Psychiatry 2021 23;12:740094. Epub 2021 Aug 23.

Faculty of Psychology, Second Military Medical University, Shanghai, China.

The COVID-19 pandemic has greatly impacted people's life across the globe. In a public health crisis, rural adolescents are more prone to mental health problems. The current study aimed to investigate the prevalence of depressive symptoms among Chinese rural adolescents during the COVID-19 outbreak, and examine the association between perceived social support and depressive symptoms and its underlying mechanisms. Perceived Social Support Scale, UCLA Loneliness Scale, Patient Health Questionnaire-9 were administrated to 826 rural adolescents from Anhui Province, China, amid the COVID-19 crisis. Mackinnon's four-step procedure was employed to examine the mediating effect, while Hayes PROCESS macro was utilized to test the moderated mediation model. The results showed the rate of depressive symptoms among rural adolescents in China was 77.6% during the outbreak of COVID-19. Female left-behind students and non-left-behind students from disrupted families experienced more depressive symptoms (all < 0.05). Loneliness mediated the association between perceive social support and depressive symptoms and the indirect effect was stronger in left-behind adolescents in comparison to non-left-behind adolescents during the COVID-19 pandemic. Depressive symptoms are extremely prevalent among Chinese rural adolescents during the COVID-19 outbreak, and perceived social support plays a protective role against depressive symptoms. Chinese rural adolescents, especially left-behind students, could benefit from the interventions aimed at enhancing the perceived social support and reducing loneliness during the COVID-19 pandemic.
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http://dx.doi.org/10.3389/fpsyt.2021.740094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8420998PMC
August 2021

Design of a self-centring drill bit for orthopaedic surgery: A systematic comparison of the drilling performance.

J Mech Behav Biomed Mater 2021 11 25;123:104727. Epub 2021 Aug 25.

Department of Mechanical Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 1138656, Japan.

Bone drilling is an indispensable and demanding operation among many orthopaedic operations. A dedicated drill bit that can achieve low-trauma and self-centring drilling is in urgent need. In this study, a three-step orthopaedic low-traumatic drill bit design was proposed. In order to evaluate the drilling performance of the proposed drill, comprehensive comparison tests were carried out with various commercial medical drills in terms of skiving force, thrust force, temperature rise, and surface quality. The experimental results show that the proposed three-step drill design with the optimal point angle, a small chisel edge, transition arc and web thinning can obtain lower and more stable thrust force, slighter bending force, smaller temperature rise, and higher hole quality compared with the commercial drill bits. The proposed drill shows satisfactory drilling performance and has great application potential in clinical surgery.
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http://dx.doi.org/10.1016/j.jmbbm.2021.104727DOI Listing
November 2021
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