Publications by authors named "Jianguo Wang"

354 Publications

Integrated Hierarchy-Correlation Model for Evaluating Water-Driven Oil Reservoirs.

ACS Omega 2021 Dec 9;6(50):34460-34469. Epub 2021 Dec 9.

State Key Laboratory of Petroleum Resources and Prospecting, China University of Petroleum (Beijing), Beijing 102249, China.

With the increasing demands on energy and environmental domains, not only high oil production but also its accurate quantification has become one of the most important topics in academia and industry. This paper initially proposes a comprehensive workflow in which an integrated hierarchy-correlation model is used to thoroughly evaluate the influences of all relevant reservoir parameters on the ultimate oil recovery for water-flooding oil reservoirs. More specifically, the analytic hierarchy process, grey relation, and entropy weight are combined through the multiplicative weighting method to quantitatively describe the production parameters. Accordingly, novel multivariable linear and nonlinear correlations are developed to predict the production performance and validated through comparisons with numerical reservoir simulations. Seven factors, including five reservoir parameters, namely, permeability and its contrast, porosity, thickness, and saturation, and two production parameters, namely, the injection-production ratio and the operating pressure, have been identified as the most influential factors on recovery performances and thus are employed in the proposed correlations to predict the ultimate oil recovery factor. The results obtained by the proposed method are quite close to the real-time simulation data, while the accuracy is retained. The numerical results show that the recovery factors of water-flooding oil reservoirs are about 33.5-59.5%, and the corresponding linear and nonlinear correlation coefficients are 0.903 and 0.789, respectively. In comparison with the numerical simulation, the approximation error by the linear correlation is about 0.5%, which is lower than that of nonlinear correlation, for example, 12.3%. This study will be beneficial to analyze the reservoir-related parameters and provide a useful tool for rapid production performance evaluation of the water-flooding production scenario.
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http://dx.doi.org/10.1021/acsomega.1c04631DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697404PMC
December 2021

NEFA Promotes Autophagosome Formation through Modulating PERK Signaling Pathway in Bovine Hepatocytes.

Animals (Basel) 2021 Nov 28;11(12). Epub 2021 Nov 28.

College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.

During the perinatal period, the abnormally high plasma non-esterified fatty acids (NEFA) concentration caused by the negative energy balance (NEB) can impose a significant metabolic stress on the liver of dairy cows. Endoplasmic reticulum (ER) stress is an important adaptive response that can serve to maintain cell homeostasis in the event of stress. The protein kinase R-like endoplasmic reticulum kinase (PERK) pathway is the most rapidly activated cascade when ER stress occurs in cells and has an important impact on the regulation of hepatic lipid metabolism and autophagy modulation. However, it is unknown whether NEFA can affect autophagy through modulating the PERK pathway, under NEB conditions. In this study, we provide evidence that NEFA treatment markedly increased lipid accumulation, the phosphorylation level of PERK and eukaryotic initiation factor 2α (eIF2α), and the expression of glucose-regulated protein 78 (Grp78), activating transcription factor 4 (ATF4), and C/EBP homologous protein (CHOP). More importantly, NEFA treatment can cause a substantial increase in the protein levels of autophagy-related gene 7 (ATG7), Beclin-1 (BECN1), sequestosome-1 (p62), and microtubule-associated protein 1 light chain 3 (LC3)-II, and in the number of autophagosomes in primary bovine hepatocytes. The addition of GSK2656157 (PERK phosphorylation inhibitor) can significantly inhibit the effect of NEFA on autophagy and can further increase lipid accumulation. Overall, our results indicate that NEFA could promote autophagy via the PERK pathway in bovine hepatocytes. These findings provide novel evidence about the potential role of the PERK signaling pathway in maintaining bovine hepatocyte homeostasis.
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http://dx.doi.org/10.3390/ani11123400DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697899PMC
November 2021

Changes in Acute-Phase Proteins in Plasma during the Periparturient Period of Dairy Goats.

Vet Sci 2021 Dec 8;8(12). Epub 2021 Dec 8.

College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China.

The present study was conducted regarding four acute-phase proteins (APPs) including C-reactive protein (CRP), ceruloplasmin (CP), serum amyloid A (SAA), and haptoglobin (HP) in dairy goats during the periparturient period. The aim of this study was to detect the changes in APPs in plasma during the periparturient period of healthy dairy goats. Guanzhong dairy goats with no other symptoms ( = 15) were selected on the basis of their blood calcium (Ca) and β-hydroxybutyrate (BHBA) concentration. The plasma was collected once a week for ±3 weeks delivery. The concentrations of the four APPs mentioned above were determined using goat-specific ELISA kits. The results showed the CRP level in plasma decreased from 3 weeks to 1 week antepartum and increased later until 1 week postpartum and then decreased to a similar level with antepartum between 1 and 3 weeks postpartum. The content of CP showed a decline in 3 weeks before parturition and an upward trend between 1 week antepartum and 3 weeks postpartum. The SAA concentration decreased from 3 weeks antepartum to 2 weeks postpartum and rebounded later. The level of HP decreased during 3 weeks before parturition and increased until 1 week postpartum, then reached a stable value. Clear variation range and rules of APPs contribute to perinatal health monitoring of dairy goats.
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http://dx.doi.org/10.3390/vetsci8120311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707259PMC
December 2021

A novel polymerase β inhibitor from phage displayed peptide library augments the anti-tumour effects of temozolomide on colorectal cancer.

J Chemother 2021 Dec 6:1-10. Epub 2021 Dec 6.

Qinhuai Medical District, Jinlin Hospital of Nanjing University, Nanjing, China.

The therapeutic efficacy of TMZ, a common used drug for chemotherapy, is limited by the resistance from colorectal cancer cells. Base excision repair (BER) pathway has been identified as one of the reasons for drug resistance. By blocking Polβ-dependent BER (Base Excision Repair) pathway, the efficacy of TMZ treatment can be improved greatly. Several Polβ inhibitors that have been identified could not become approved drugs due to lack of potency or specificity. To find therapeutic candidates with exquisite specificity and high affinity to Polβ, phage display technology was used in the current research. We screened out a candidate Polβ inhibitor, 10 D, that can inhibit the activity of Polβand SP-BER (Short-Patch Base excision Repair) pathway. Co-treatment with 10 D enhanced the sensitivity of colorectal cancer (CRC) cells to TMZ both and . Our data suggested that the novel Polβ inhibitor we identified can improve TMZ efficacy and optimize CRC chemotherapy.
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http://dx.doi.org/10.1080/1120009X.2021.2009987DOI Listing
December 2021

Mapping single-cell-resolution cell phylogeny reveals cell population dynamics during organ development.

Nat Methods 2021 12 2;18(12):1506-1514. Epub 2021 Dec 2.

MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.

Mapping the cell phylogeny of a complex multicellular organism relies on somatic mutations accumulated from zygote to adult. Available cell barcoding methods can record about three mutations per barcode, enabling only low-resolution mapping of the cell phylogeny of complex organisms. Here we developed SMALT, a substitution mutation-aided lineage-tracing system that outperforms the available cell barcoding methods in mapping cell phylogeny. We applied SMALT to Drosophila melanogaster and obtained on average more than 20 mutations on a three-kilobase-pair barcoding sequence in early-adult cells. Using the barcoding mutations, we obtained high-quality cell phylogenetic trees, each comprising several thousand internal nodes with 84-93% median bootstrap support. The obtained cell phylogenies enabled a population genetic analysis that estimates the longitudinal dynamics of the number of actively dividing parental cells (Np) in each organ through development. The Np dynamics revealed the trajectory of cell births and provided insight into the balance of symmetric and asymmetric cell division.
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http://dx.doi.org/10.1038/s41592-021-01325-xDOI Listing
December 2021

Assembly of Silicalite-1 Crystals Like Toy Lego Bricks into One-, Two-, and Three-Dimensional Architectures for Enhancing Its Adsorptive Separation and Catalytic Performances.

ACS Appl Mater Interfaces 2021 Dec 26;13(48):58085-58095. Epub 2021 Nov 26.

State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, 27 South Taoyuan Road, Taiyuan 030001, China.

Many researchers have contributed to the assembly of zeolitic nanosheets and nanocrystallites into three-dimensional (3D) networks as it can remarkably improve the catalytic and/or adsorptive performances of zeolites. However, the applications of these synthesized materials are seriously limited because of low hydrothermal stability. A highly interesting strategy, but a great challenge, is the alignment of well-crystallized zeolite crystals into desirable architectures. Here, well-crystallized silicalite-1 crystals are assembled like toy Lego bricks into one-dimensional (1D), two-dimensional (2D), and three-dimensional (3D) architectures, and the assembly mechanism is investigated by combining elaborate experiments, in situ spectroscopy, and theoretical calculations. A 1D architecture was formed by stacking crystals along the axis with the assistance of ethanol that is selectively adsorbed on (100) and (001) crystal facets. Such adsorption increases the condensation energy barriers along and axes, but facilitates the condensation between (010) facets. The assembly of the crystals into well-arrayed 2D architectures is achieved using both ethanol and benzaldehyde because of their preferable adsorption on the (001) facet. When an amphiphilic copolymer (P123) was further added in the gel along with the substitution of ethanol by 1-propanol, a 3D network was fabricated by the agglomeration and self-pillaring of the 2D Lego bricks possibly with P123 aggregates as the substrate matrix. Excitingly, upon alignment of crystals into 2D architectures, the adsorptive selectivity of 1-butanol (2 wt %) to water of silicalite-1 increases by 45.3 times, while into 3D networks, the catalytic activity for the Beckmann rearrangement of cyclohexanone oxime elevates by 79% along with a great enhancement of catalytic stability.
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http://dx.doi.org/10.1021/acsami.1c15226DOI Listing
December 2021

A synergy between the push-pull electronic effect and twisted conformation for high-contrast mechanochromic AIEgens.

Mater Horiz 2021 Feb 15;8(2):630-638. Epub 2020 Dec 15.

AIE Research Center, Shaanxi Key Laboratory of Phytochemistry, College of Chemistry and Chemical Engineering, Baoji University of Arts and Sciences, Baoji 721013, China.

Mechanochromic (MC) luminogens in response to external stimulus have shown promising applications as pressure sensors and memory devices. Meanwhile, research on their underlying mechanism is still in the initial stage. Here, three pyridinium-functionalized tetraphenylethylenes bearing n-pentyloxy, hydrogen and nitro groups, namely TPE-OP, TPE-H and TPE-NO, are designed to systematically investigate the influence of the push-pull electronic effect and molecular conformation on MC luminescence. Upon anisotropic grinding and isotropic hydrostatic compression, TPE-OP with strong intramolecular charge transfer (ICT) affords the best MC behavior among them. Analysis of three polymorphs of TPE-H clearly indicates that planarization of the molecular conformation plays an important role in their bathochromic shifts under mechanical stimuli. Theoretical calculations also verify that high twisting stress of AIEgens can be released under high pressure. This study presents a mechanistic insight into MC behaviour and an effective strategy to achieve high-contrast MC luminescence.
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http://dx.doi.org/10.1039/d0mh01251cDOI Listing
February 2021

Structure and pro-inflammatory activities of bran polysaccharides from a novel wheat kernel.

J Food Biochem 2022 Jan 22;46(1):e14008. Epub 2021 Nov 22.

College of Food Science and Engineering, Northwest A&F University, Yangling, China.

In this study, the structure and pro-inflammatory activities of water-soluble wheat bran polysaccharides (WBP) were evaluated. WBP were heteropolysaccharides consisting 60.34% arabinoxylan as the main component and 31.80% mannose residues characterized with the instrumental analyses. The result of cellular experiment displayed that WBP had significant pro-inflammatory activities by increasing the concentration of nitric oxide (NO) and up-regulating the inflammatory cytokine expressions of inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), cyclooxygenase-2 (COX-2), and tumor necrosis factor-α (TNF-α). WBP mediated macrophages RAW 264.7 pro-inflammatory response through phosphatidylinositol 3 hydroxykinase/protein kinase B (PI3K/Akt) signaling pathway by significantly promoting Akt and phosphoinositide-dependent kinase 1 (PDK1) phosphorylations. Meanwhile, the expression of related phosphorylated proteins JNK and ERK1/2 was significantly up-regulated which suggested that WBP played pro-inflammatory roles by activating mitogen-activated protein kinases (MAPKs) signaling pathway. PRACTICAL APPLICATIONS: In recent years, wheat bran generally has the phenomenon of high yield and low utilization rate. Wheat bran has rich nutritional value and contains a lot of effective biologically active substances. Based on our findings, the water-soluble polysaccharides extracted from wheat bran have significant effects on regulating immunity and can be utilized as sources of natural immune modulators. The research can develop new functions of wheat bran polysaccharides, and improve processing utilization rate and product added value.
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http://dx.doi.org/10.1111/jfbc.14008DOI Listing
January 2022

Scolytus jiulianshanensis, a new species of bark beetle (Coleoptera: Curculionidae: Scolytinae) from elm in China.

Zootaxa 2021 Oct 21;5057(2):295-300. Epub 2021 Oct 21.

School of Agricultural Sciences, Jiangxi Agricultural University, Nanchang, Jiangxi, 330045, P. R. China. .

A new species of bark beetle, Scolytus jiulianshanensis Zhang, Li Smith sp. nov., from Jiangxi, China is described and illustrated. This new species was collected from dead elm (Ulmus sp.) trees. A DNA barcoding sequence of this species is provided. The new species is distinguished from other Asian Scolytus species by the apical margins of ventrites 3 and 4 of the male each armed with a broad median tubercle and lacking ventral spines.
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http://dx.doi.org/10.11646/zootaxa.5057.2.9DOI Listing
October 2021

The distinct responsiveness of cytokeratin 19-positive hepatocellular carcinoma to regorafenib.

Cell Death Dis 2021 11 16;12(12):1084. Epub 2021 Nov 16.

Department of Hepatobiliary and Pancreatic Surgery, Center for Integrated Oncology and Precision Medicine, the Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Cytokeratin 19-positive (CK19+) hepatocellular carcinoma (HCC) is an aggressive subtype characterized by early recurrence and chemotherapy tolerance. However, there is no specific therapeutic option for CK19+ HCC. The correlation between tumor recurrence and expression status of CK19 were studied in 206 patients undergoing liver transplantation for HCC. CK19-/+ HCC cells were isolated to screen effective antitumor drugs. The therapeutic effects of regorafenib were evaluated in patient-derived xenograft (PDX) models from 10 HCC patients. The mechanism of regorafenib on CK19+ HCC was investigated. CK19 positiveness indicated aggressiveness of tumor and higher recurrence risk of HCC after liver transplantation. The isolated CK19+ HCC cells had more aggressive behaviors than CK19- cells. Regorafenib preferentially increased the growth inhibition and apoptosis of CK19+ cells in vitro, whereas sorafenib, apatinib, and 5-fluorouracil did not. In PDX models from CK19-/+ HCC patients, the tumor control rate of regorafenib achieved 80% for CK19+ HCCs, whereas 0% for CK19- HCCs. RNA-sequencing revealed that CK19+ cells had elevated expression of mitochondrial ribosomal proteins, which are essential for mitochondrial function. Further experiments confirmed that regorafenib attenuated the mitochondrial respiratory capacity in CK19+ cells. However, the mitochondrial respiration in CK19- cells were faint and hardly repressed by regorafenib. The mitochondrial respiration was regulated by the phosphorylation of signal transducer and activator of transcription 3 (STAT3), which was inhibited by regorafenib in CK19+ cells. Hence, CK19 could be a potential marker of the therapeutic benefit of regorafenib, which facilitates the individualized therapy for HCC. STAT3/mitochondria axis determines the distinct response of CK19+ cells to regorafenib treatment.
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http://dx.doi.org/10.1038/s41419-021-04320-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8595883PMC
November 2021

Study on the Flow State of Circulating Cooling Water for the Industrial Heat Exchange Tube in the Electromagnetic Anti-Fouling Process.

ACS Omega 2021 Nov 20;6(43):28515-28527. Epub 2021 Oct 20.

School of Technology, Beijing Forestry University, Beijing 100083, China.

Comparing with the traditional chemical and physical method, the electromagnetic water treatment technology draws more attention of researchers for its advantages of easy application, small investment, low cost, and being pollution free in recent years. However, due to the less study of the formation process and adhesion of fouling on the surface of heat exchange equipment, the electromagnetic anti-fouling performance cannot be well evaluated. This paper studies the numerical simulation of the flow states of circulating cooling water in heat exchange tubes with a straight shape and U-shaped ones and analyzes the experimental data of fouling resistance on heat transfer surface under the action of 0.5, 0.75, 1, and 1.5 kHz electromagnetic fields. The variations in the velocity field and pressure field at various points in heat exchange tubes declare that the velocity of the circulating cooling water is smaller in the outlet of the pipeline. The change of the circulating cooling water flow state with the pipeline shape causes a certain impact on fluid velocity, and the pressure value at the outlet is larger. It is obtained that the flow velocity in the area with high surface pressure of circulating cooling water is relatively small. The experimental results indicate that the fouling resistance on the surface of the magnetic heat exchange tube is smaller than that of the nonmagnetic one. The anti-fouling efficiency in 0.5, 0.75, 1, and 1.5 kHz magnetic and contrast experiments are 46.8, 84.8, 91.2, and 63.6%, respectively. Better anti-fouling performances are obtained under the action of about 1 kHz electromagnetic frequency. The induction period of fouling on the heat exchange surface is lengthened under electromagnetic fields. All these studies are of significant importance to further understand the formation process and adhesion of fouling on the surface of heat exchange equipment, as well as to better evaluate the electromagnetic anti-fouling performance.
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http://dx.doi.org/10.1021/acsomega.1c02193DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567286PMC
November 2021

Charge Neutralization Strategy to Construct Salt-Tolerant and Cell-Permeable Nanoprobes: Application in Ratiometric Sensing and Imaging of Intracellular pH.

Anal Chem 2021 11 4;93(45):15159-15166. Epub 2021 Nov 4.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.

Intracellular pH homeostasis is essential for the survival and function of biological cells. Negatively charged molecular probes, such as pyranine (HPTS), tend to exhibit poor salt tolerance and unsatisfactory cell permeability, limiting their widespread use in intracellular assays. Herein, we explored a charge neutralization strategy using multicharged cationic nanocarriers for an efficient and stable assembly with the pH-sensitive HPTS. Through immobilization and neutralization with poly(allylamine hydrochloride)-stabilized red-emitting gold nanoclusters (PAH-AuNCs), the resulting nanoprobes (HPTS-PAH-AuNCs) offered improved salt tolerance, satisfactory cell permeability, and dual-emission properties. The fluorescence ratio exhibited a linear response over the pH range of 3.0-9.0. Moreover, the proposed HPTS-PAH-AuNCs were successfully applied to determine and visualize lysosomal pH variations in living cells, which indicated great potential for biosensing and bioimaging applications in living systems. Benefiting from the charge neutralization strategy, various types of probes can be expected to achieve broader analytical applications.
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http://dx.doi.org/10.1021/acs.analchem.1c03629DOI Listing
November 2021

Symbolic Transformer Accelerating Machine Learning Screening of Hydrogen and Deuterium Evolution Reaction Catalysts in MAZ Materials.

ACS Appl Mater Interfaces 2021 Nov 21;13(43):50878-50891. Epub 2021 Oct 21.

Institute of Industrial Catalysis, State Key Laboratory Breeding Base of Green-Chemical Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310032, P. R. China.

Two-dimensional (2D) materials have been developed into various catalysts with high performance, but employing them for developing highly stable and active nonprecious hydrogen evolution reaction (HER) catalysts still encounters many challenges. To this end, the machine learning (ML) screening of HER catalysts is accelerated by using genetic programming (GP) of symbolic transformers for various typical 2D MAZ materials. The values of the Gibbs free energy of hydrogen adsorption (Δ) are accurately and rapidly predicted via extreme gradient boosting regression by using only simple GP-processed elemental features, with a low predictive root-mean-square error of 0.14 eV. With the analysis of ML and density functional theory (DFT) methods, it is found that various electronic structural properties of metal atoms and the p-band center of surface atoms play a crucial role in regulating the HER performance. Based on these findings, NbSiN and VSiN are discovered to be active catalysts with thermodynamical and dynamical stability as Δ approaches to zero (-0.041 and 0.024 eV). In addition, DFT calculations reveal that these catalysts also exhibit good deuterium evolution reaction (DER) performance. Overall, a multistep workflow is developed through ML models combined with DFT calculations for efficiently screening the potential HER and DER catalysts from 2D materials with the same crystal prototype, which is believed to have significant contribution to catalyst design and fabrication.
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http://dx.doi.org/10.1021/acsami.1c13236DOI Listing
November 2021

Deletion of PRAK Mitigates the Mitochondria Function and Suppresses Insulin Signaling in C2C12 Myoblasts Exposed to High Glucose.

Front Pharmacol 2021 4;12:698714. Epub 2021 Oct 4.

Department of Surgery, Roger Williams Medical Center, Boston University School of Medicine, Boston, MA, United States.

p38 regulated/activated protein kinase (PRAK) plays a crucial role in modulating cell death and survival. However, the role of PRAK in the regulation of metabolic stress remains unclear. We examined the effects of PRAK on cell survival and mitochondrial function in C2C12 myoblasts in response to high glucose stresses. PRAK of C2C12 myoblasts was knocked out by using CRISPR/Cas-9 genome editing technology. Both wild type and PRAK C2C12 cells were exposed to high glucose at the concentration of 30 mmol/L to induce metabolic stress. The effect of irisin, an adipomyokine, on both wild type and PRAK cells was determined to explore its relationship with RPAK. Cell viability, ATP product, glucose uptake, mitochondrial damage, and insulin signaling were assessed. PRAK knockout decreased C2C12 viability in response to high glucose stress as evident by MTT assay in association with the reduction of ATP and glucose uptake. PRAK knockout enhanced apoptosis of C2C12 myoblasts in response to high glucose, consistent with an impairment in mitochondrial function, by decreasing mitochondrial membrane potential. PRAK knockout induced impairment of mitochondrial and cell damage were rescued by irisin. PRAK knockout caused decrease in phosphorylated PI3 kinase at Tyr 485, IRS-1 and AMPKα and but did not affect non-phosphorylated PI3 kinase, IRS-1 and AMPKα signaling. High glucose caused the further reduction of phosphorylated PI3 kinase, IRS-1 and AMPKα. Irisin treatment preserved phosphorylated PI3 kinase, IRS-1by rescuing PRAK in high glucose treatment. Our finding indicates a pivotal role of PRAK in preserving cellular survival, mitochondrial function, and high glucose stress.
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http://dx.doi.org/10.3389/fphar.2021.698714DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521062PMC
October 2021

Synthesis and Properties of Aza-Ovalene with Six Zigzag Edges.

Org Lett 2021 Nov 13;23(21):8640-8644. Epub 2021 Oct 13.

School of Chemistry and Chemical Engineering, Inner Mongolia University, 235 West University Street, Hohhot 010021, China.

Ovalene's nitrogenated derivative with all zigzag edges and nitrogen atom doping at the periphery has been developed via one-step nitrogenation of formylbisanthene. Because of nitrogen incorporation, these molecules show greatly decreased highest occupied molecular orbital levels, enhanced intermolecular interactions, and a reversible acid response. Aza-ovalene also exhibits a diatropic ring current along the periphery. This work provides rare examples of all-zigzag-edged N-polycyclic aromatic hydrocarbons.
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http://dx.doi.org/10.1021/acs.orglett.1c03354DOI Listing
November 2021

Molecular phenotypes reveal heterogeneous engraftments of patient-derived hepatocellular carcinoma xenografts.

Chin J Cancer Res 2021 Aug;33(4):470-479

Department of Hepatobiliary and Pancreatic Surgery, The Center for Integrated Oncology and Precision Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.

Objective: Patient-derived xenograft (PDX) models provide a promising preclinical platform for hepatocellular carcinoma (HCC). However, the molecular features associated with successful engraftment of PDX models have not been revealed.

Methods: HCC tumor samples from 76 patients were implanted in immunodeficient mice. The molecular expression was evaluated by immunohistochemistry. Patient and tumor characteristics as well as tumor molecular expressions were compared for PDX engraftment using the Chi-square test. The independent prediction parameters were identified by logistic regression analyses.

Results: The engraftment rate for PDX models from patients with HCC was 39.47% (30/76). Tumors from younger patients and patients with elevated preoperative alpha-fetoprotein level had higher engraftment rates. Tumors with poor differentiation and vascular invasion were related to engraftment success. The positive expression of CK19, CD133, glypican-3 (GPC3), and Ki67 in tumor samples was associated with engraftment success. Logistic regression analyses indicated that GPC3 and Ki67 were two of the strongest predictors of PDX engraftment. Tumors with GPC3/Ki67 phenotypes showed heterogeneous engraftment rates, with 71.9% in GPC3/Ki67 tumors, 30.8% in GPC3/Ki67 tumors, 15.0% in GPC3/Ki67 tumors, and 0 in GPC3/Ki67 tumors.

Conclusions: Successful engraftment of HCC PDXs was significantly related to molecular features. Tumors with the GPC3/Ki67 phenotype were the most likely to successfully establish HCC PDXs.
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http://dx.doi.org/10.21147/j.issn.1000-9604.2021.04.04DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435819PMC
August 2021

Dual effect of the coordination field and sulphuric acid on the properties of a single-atom catalyst in the electrosynthesis of HO.

Phys Chem Chem Phys 2021 Sep 29;23(37):21338-21349. Epub 2021 Sep 29.

Institute of Industrial Catalysis, State Key Laboratory Breeding Base of Green-Chemical Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310032, P. R. China.

Electrocatalytic synthesis of hydrogen peroxide (HO) the two-electron oxygen reduction reaction (2e ORR) is the ideal solution for on-site HO production. Herein, we propose a new strategy for creating new 2e ORR catalysts by introducing electron-deficient B atoms and electron-rich N atoms to regulate the coordination field of metal ions on a graphene substrate. Through the first-principles density functional theory (DFT) calculations, NiNB-h was screened out as it had a low overpotential (0.12 V) for 2e ORR. The Bader charge analysis revealed that B atoms increased the charge density of Ni atoms, leading to moderate binding of O. Furthermore, the combination of molecular dynamic (AIMD) calculations and DFT calculations in an HSO environment revealed a new reaction mechanism of HO synthesis, involving proton-transfer between activated O and HSO. Moreover, the rate-determining step (0.63 eV) of HO desorption in the presence of HSO was different from that of OOH* protonation (0.45 eV) under the gas phase. This difference is attributed to the hydrogen-bond network in the acid solution.
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http://dx.doi.org/10.1039/d1cp03189aDOI Listing
September 2021

Association between novel variants in BMPR1B gene and litter size in Mongolia and Ujimqin sheep breeds.

Reprod Domest Anim 2021 Dec 24;56(12):1562-1571. Epub 2021 Sep 24.

The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China.

Prolificacy is an important trait of animals, specifically for sheep. The Bone morphogenetic protein receptor 1B (BMPR1B) is a major gene affecting the litter size of many sheep breeds. The well-known FecB mutation (Q249R) was associated fully with the hyper prolific phenotype of Booroola Merino. However, the identification of variation in all exonic regions of BMPR1B was rare. In this study, we sequenced all exonic regions of BMPR1B gene of Mongolia sheep breed, and ten novel variants were detected by direct sequencing. Among them, the litter size of the Mongolia ewes with the CC genotype was significantly higher (0.34 additional lambs, p < .05) than those with the TT genotype of the g.29346567C>T single nucleotide polymorphism (SNP). The litter size of the Mongolia ewes with the TT genotype was significantly higher (0.19 additional lambs, p < .05 and .31 additional lambs, p < .01, respectively) than those with the GT and GG genotypes of the c.1470G>T SNP. The silent c.1470G>T mutation is predicted to increase the stability of the mRNA secondary structure through reducing minimum free energy and is predicted to change the mRNA secondary structure of BMPR1B. Our findings may give potentially useful genetic markers for increasing litter size in sheep.
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http://dx.doi.org/10.1111/rda.14020DOI Listing
December 2021

Experimental and theoretical study on the propagation characteristics of stress wave in filled jointed rock mass.

PLoS One 2021 9;16(9):e0253392. Epub 2021 Sep 9.

School of Civil and Hydraulic Engineering, Hefei University of Technology, Hefei, Anhui, China.

This study is to theoretically and experimentally investigate the propagation of stress waves in the filled joint set. The time-domain recursive method is used to derive the propagation equations in the filled joint set, and the filled joints are further simplified into structural planes without joint thickness. The split-Hopkinson rock bar is modified to simulate P wave propagation normally across the parallel filled joints. The relationship among stress-closure curve, joint specific stiffness, transmission coefficient and loading rate is analyzed. The results show that, for the rock mass with a single joint, both the joint specific stiffness and transmission coefficient of different filling materials increase with loading rate. More serious particle breakage of the filling materials leads to lower joint specific stiffness and transmission coefficient. For the rock mass with two joints, the joint specific stiffness of each joint affects the transmission coefficient of the filled joint set. It is found that our theoretical calculations are basically consistent with the experimental ones, and the joint specific stiffness can well characterize the propagation behavior of stress wave in the filled parallel rock joints.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253392PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428713PMC
November 2021

The metabolic flexibility of quiescent CSC: implications for chemotherapy resistance.

Cell Death Dis 2021 09 4;12(9):835. Epub 2021 Sep 4.

Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Quiescence has been observed in stem cells (SCs), including adult SCs and cancer SCs (CSCs). Conventional chemotherapies mostly target proliferating cancer cells, while the quiescent state favors CSCs escape to chemotherapeutic drugs, leaving risks for tumor recurrence or metastasis. The tumor microenvironment (TME) provides various signals that maintain resident quiescent CSCs, protect them from immune surveillance, and facilitates their recurrence potential. Since the TME has the potential to support and initiate stem cell-like programs in cancer cells, targeting the TME components may prove to be a powerful modality for the treatment of chemotherapy resistance. In addition, an increasing number of studies have discovered that CSCs exhibit the potential of metabolic flexibility when metabolic substrates are limited, and display increased robustness in response to stress. Accompanied by chemotherapy that targets proliferative cancer cells, treatments that modulate CSC quiescence through the regulation of metabolic pathways also show promise. In this review, we focus on the roles of metabolic flexibility and the TME on CSCs quiescence and further discuss potential treatments of targeting CSCs and the TME to limit chemotherapy resistance.
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http://dx.doi.org/10.1038/s41419-021-04116-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418609PMC
September 2021

MRI-Radiomics Prediction for Cytokeratin 19-Positive Hepatocellular Carcinoma: A Multicenter Study.

Front Oncol 2021 12;11:672126. Epub 2021 Aug 12.

Department of Hepatobiliary and Pancreatic Surgery, The Center of Integrated Oncology and Precision Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer and has poor prognosis. Cytokeratin (CK)19-positive (CK19+) HCC is especially aggressive; early identification of this subtype and timely intervention can potentially improve clinical outcomes. In the present study, we developed a preoperative gadoxetic acid-enhanced magnetic resonance imaging (MRI)-based radiomics model for noninvasive and accurate classification of CK19+ HCC. A multicenter and time-independent cohort of 257 patients were retrospectively enrolled (training cohort, n = 143; validation cohort A, n = 75; validation cohort B, n = 39). A total of 968 radiomics features were extracted from preoperative multisequence MR images. The maximum relevance minimum redundancy algorithm was applied for feature selection. Multiple logistic regression, support vector machine, random forest, and artificial neural network (ANN) algorithms were used to construct the radiomics model, and the area under the receiver operating characteristic (AUROC) curve was used to evaluate the diagnostic performance of corresponding classifiers. The incidence of CK19+ HCC was significantly higher in male patients. The ANN-derived combined classifier comprising 12 optimal radiomics features showed the best diagnostic performance, with AUROCs of 0.857, 0.726, and 0.790 in the training cohort and validation cohorts A and B, respectively. The combined model based on multisequence MRI radiomics features can be used for preoperative noninvasive and accurate classification of CK19+ HCC, so that personalized management strategies can be developed.
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http://dx.doi.org/10.3389/fonc.2021.672126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406635PMC
August 2021

Oxidative status in dairy goats: periparturient variation and changes in subclinical hyperketonemia and hypocalcemia.

BMC Vet Res 2021 Jul 6;17(1):238. Epub 2021 Jul 6.

College of Veterinary Medicine, Northwest A&F University, 712100, Yangling, Shaanxi, China.

Background: A better comprehension of the redox status during the periparturient period may facilitate the development of management and nutritional solutions to prevent subclinical hyperketonemia (SCHK) and subclinical hypocalcemia (SCHC) in dairy goats. We aimed to evaluate the variation in the redox status of dairy goats with SCHK and SCHC during their periparturient periods. Guanzhong dairy goats (n = 30) were assigned to SCHK (n = 10), SCHC (n = 10), and healthy (HEAL, n = 10) groups based on their blood β-hydroxybutyrate (BHBA) and calcium (Ca) concentrations. Blood were withdrawn from goats every week from 3 weeks before the expected parturition date to 3 weeks post-kidding. On the same day, the body condition scores (BCS) were evaluated, and the milk yield was recorded for each goat. The metabolic profile parameters and the indicators of oxidative status were determined by using the standard biochemical techniques.

Results: In comparison with the HEAL goats, SCHK and SCHC goats presented with a more dramatic decline of BCS post-kidding and a significant decrease in the milk yield at 2- and 3-weeks postpartum, ignoring the obvious increase at 1-week postpartum. The levels of non-esterified fatty acids (NEFA) peaked at parturition, exhibiting significantly higher levels from 1-week prepartum to the parturition day in the SCHK and SCHC groups. The malondialdehyde (MDA) concentration was increased in the SCHK goats from 1-week antepartum until 3-weeks postpartum, with its concentration being significantly higher in the SCHC goats at parturition. The hydrogen peroxide (HO) concentration was significantly lower in the SCHK and SCHC goats from 2-weeks antepartum to 1-week post-kidding. The total antioxidant capacity (T-AOC) and the superoxide dismutase (SOD) level were decreased at 1-week antepartum in the SCHK and SCHC goats, respectively. The glutathione peroxidase (GSH-Px) level was increased in the SCHK and SCHC goats during the early lactation period.

Conclusions: The SCHK and SCHC goats exerted more efforts to maintain their redox homeostasis and to ensure the production performance than the HEAL goats during their periparturient period, probably owing to more intense fat mobilization and lipid peroxidation in the former.
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http://dx.doi.org/10.1186/s12917-021-02947-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258950PMC
July 2021

Surrogate Indexes of Insulin Resistance in Dairy Goats: Transitional Variation in Subclinical Hyperketonemia.

Vet Sci 2021 Jun 6;8(6). Epub 2021 Jun 6.

College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.

Background: Dairy goats are highly susceptible to subclinical hyperketonemia (SCHK) during the transition period. This study aimed to compare the variation in metabolic parameters and surrogate indexes of insulin resistance (sIR) between goats with SCHK and clinically healthy (HEAL) goats during the transition period.

Methods: Twenty Guanzhong dairy goats were assorted to HEAL ( = 10) and SCHK ( = 10) groups according to the blood β-hydroxybutyrate (BHBA) concentrations. The blood samples were taken from the jugular vein of each goat at -3, -2, -1, 0 (partum), +1, +2, and +3 weeks relative to kidding to analyses GLU and INS. The sIR was calculated from blood metabolic parameters.

Results: Compared with the HEAL goats, the insulin concentrations were significantly higher in SCHK goats during the first three weeks postpartum. The QUICKI, revised QUICKI (RQUICKI), and RQUICKI were significantly lower in goats with SCHK at 1 week postpartum, while the homeostasis model assessment-IR (HOMA-IR) was significantly higher.

Conclusion: Goats with SCHK made more efforts through elevated insulin levels at early lactation than HEAL goats, thereby maintaining the normal glucose concentrations.
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http://dx.doi.org/10.3390/vetsci8060102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227330PMC
June 2021

Correlations Between the Characteristics of Alternative Splicing Events, Prognosis, and the Immune Microenvironment in Breast Cancer.

Front Genet 2021 14;12:686298. Epub 2021 Jun 14.

Department of General Surgery, Xiangtan Central Hospital, Xiangtan, China.

Objective: Alternative splicing (AS) is the mechanism by which a few genes encode numerous proteins, and it redefines the concept of gene expression regulation. Recent studies showed that dysregulation of AS was an important cause of tumorigenesis and microenvironment formation. Therefore, we performed a systematic analysis to examine the role of AS in breast cancer (Breast Cancer, BrCa) progression.

Methods: The present study included 993 BrCa patients from The Cancer Genome Atlas (TCGA) database in the genome-wide analysis of AS events. We used differential and prognostic analyses and found differentially expressed alternative splicing (DEAS) events and independent prognostic factors related to patients' overall survival (OS) and disease-free survival (DFS). We divided the patients into two groups based on these AS events and analyzed their clinical features, molecular subtyping and immune characteristics. We also constructed a splicing factor (SF) regulation network for key AS events and verified the existence of AS events in tissue samples using real-time quantitative PCR.

Results: A total of 678 AS events were identified as differentially expressed, of which 13 and 10 AS events were independent prognostic factors of patients' OS and DFS, respectively. Unsupervised clustering analysis based on these prognostic factors indicated that the Cluster 1 group had a better prognosis and more immune cell infiltration. SFs were significantly related to the expression of AS events, and AA-RPS21 was significantly upregulated in tumors.

Conclusion: Alternative splicing expands the mechanism of breast cancer progression from a new perspective. Notably, alternative splicing may affect the patient's prognosis by affecting the infiltration of immune cells. Our research provides important guidance for subsequent studies of AS in breast cancer.
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http://dx.doi.org/10.3389/fgene.2021.686298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236959PMC
June 2021

Unusual light-driven amplification through unexpected regioselective photogeneration of five-membered azaheterocyclic AIEgen.

Chem Sci 2020 Oct 19;12(2):709-717. Epub 2020 Oct 19.

Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, State Key Laboratory of Molecular Nanoscience, Division of Life Science, Department of Chemical and Biomedical Engineering and Institute for Advanced Study, The Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong China

Developing versatile synthetic methodologies with merits of simplicity, efficiency, and environment friendliness for five-membered heterocycles is of incredible importance to pharmaceutical and material science, as well as a huge challenge to synthetic chemistry. Herein, an unexpected regioselective photoreaction to construct a fused five-membered azaheterocycle with an aggregation-induced emission (AIE) characteristic is developed under mild conditions. The formation of the five-membered ring is both thermodynamically and kinetically favored, as justified by theoretical calculation and experimental evidence. Markedly, a light-driven amplification strategy is proposed and applied in selective mitochondria-targeted cancer cell recognition and fluorescent photopattern fabrication with improved resolution. The work not only delivers the first report on efficiently generating a fused five-membered azaheterocyclic AIE luminogen under mild conditions photoreaction, but also offers deep insight into the essence of the photosynthesis of fused five-membered azaheterocyclic compounds.
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http://dx.doi.org/10.1039/d0sc04725bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179000PMC
October 2020

Pyrotinib Treatment in Patients With HER2-positive Metastatic Breast Cancer and Brain Metastasis: Exploratory Final Analysis of Real-World, Multicenter Data.

Clin Cancer Res 2021 08 10;27(16):4634-4641. Epub 2021 Jun 10.

Department of Oncology, Central Hospital of Shaoyang, Shaoyang, China.

Purpose: Patients with HER2-positive (HER2) metastatic breast cancer (MBC) have poor prognoses. Pyrotinib has shown promising antitumor activity in MBC to improve progression-free survival (PFS). However, findings based on real-world data to analyze whether pyrotinib affects overall survival (OS) remain scarce.

Experimental Design: This real-world study is an exploratory analysis of brain metastasis (BM) and the final update of our preceding study of 168 patients with HER2 MBC. PFS, OS, tumor mutation burden (TMB), clinical benefit rate (CBR), and overall response rate (ORR) were analyzed.

Results: Pyrotinib treatment led to a median PFS time of 8.00 months and a median OS of 19.07 months in the 168 participants. High TMB was associated with poor OS ( = 0.0072) and PFS ( = 0.0028). In the 39 patients with BM, the median PFS and OS were 8.67 and 13.93 months, respectively. The surgery/radiation (S/R) group of patients with BM had prolonged survival (PFS: 9.97 vs. 7.73 months = 0.19; OS: 20.67 vs. 12.43 months = 0.021) compared with the no surgery/no radiation group (NS/NR). The CBR was 58.6% (S/R) vs. 41.4% (NS/NR), while the ORR was 24.1% (S/R) vs. 31.0% (NS/NR).

Conclusions: Pyrotinib shows promise as a novel pan-HER2 tyrosine kinase inhibitor (TKI) for the treatment of BM and should be evaluated further. Surgical or radiotherapy in combination with pyrotinib was found to statistically improve OS in our cohort. TMB could be an exploratory biomarker for predicting PFS and OS, but its clinical application still needs further verification.
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http://dx.doi.org/10.1158/1078-0432.CCR-21-0474DOI Listing
August 2021

CHOP Regulates Endoplasmic Reticulum Stress-Mediated Hepatoxicity Induced by Monocrotaline.

Front Pharmacol 2021 24;12:685895. Epub 2021 May 24.

College of Veterinary Medicine, Northwest A&F University, Yangling, China.

Monocrotaline (MCT), a pyrrolizidine alkaloid, is the major toxin in , which causes cell apoptosis in humans and animals. It has been reported that the liver is a vulnerable target of MCT. However, the exact molecular mechanism of the interaction between endoplasmic reticulum (ER) stress and liver injury induced by MCT is still unclear. In this study, the cytotoxicity of MCT on primary rat hepatocytes was analyzed by a CCK-8 assay and Annexin V-FITC/PI assay. Protein expression was detected by western blotting and immunofluorescence staining. As a result, MCT significantly decreased the cell viability and mediated the apoptosis of primary rat hepatocytes. Meanwhile, MCT could also induce ER stress in hepatocytes, indicated by the expression of ER stress-related proteins, including GRP78, p-IRE1α, ATF6, p-eIF2α, ATF4, and CHOP. Pretreatment with 4-PBA, an inhibitor of ER stress, or knockdown of CHOP by siRNA could partly enhance cell viability and relieve the apoptosis. Our findings indicate that ER stress is involved in the hepatotoxicity induced by MCT, and CHOP plays an important role in this process.
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http://dx.doi.org/10.3389/fphar.2021.685895DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181757PMC
May 2021

Leader-chasing behavior in negative artificial triggered lightning flashes.

Sci Rep 2021 06 2;11(1):11598. Epub 2021 Jun 2.

School of Electrical Engineering and Automation, Wuhan University, Wuhan, 430000, China.

Two special cases of dart leader propagation were observed by the high-speed camera in the leader/return stroke sequences of a classical triggered lightning flash and an altitude-triggered lightning flash, respectively. Different from most of the subsequent return strokes preceded by only one leader, the return stroke in each case was preceded by two leaders occurring successively and competing in the same channel, which herein is named leader-chasing behavior. In one case, the polarity of the latter leader was opposite to that of the former leader and these two combined together to form a new leader, which shared the same polarity with the former leader. In the other case, the latter leader shared the same polarity with the former leader and disappeared after catching up with the former leader. The propagation of the former leader in this case seems not to be significantly influenced by the existence of the latter leader.
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http://dx.doi.org/10.1038/s41598-021-90940-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172644PMC
June 2021

[Effect of influenza virus on chemokine pathways in mice lungs and intervention of Shufeng Xuanfei Jiedu formula].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 Apr;33(4):405-409

Shanxi University of Traditional Chinese Medicine, Jinzhong 030619, Shanxi, China.

Objective: To screen the differentially expressed genes on whole expression profiles of the inflammation-related cytokines in mice infected with influenza virus by the gene chip technology, and to explore the intervention effect of Shufeng Xuanfei Jiedu formula.

Methods: Male ICR mice were divided into normal group (N group), influenza virus infective model group (M group), Oseltamivir control group (C group) and Shufeng Xuanfei Jiedu formula high, medium and low dose groups (SH, SM, SL groups) according to the random number table method, with 10 rats in each group. A mouse model of influenza virus pneumonia was reproduced by nasal drip of influenza virus strain FM1 (0.05 mL). In N group, 0.05 mL normal saline was used. In SH, SM and SL groups, Shufeng Xuanfei Jiedu formula was used 2 hours after intranasal infection (2 times, equal and 1/2 of the clinical treatment dose, approximately 3.8, 1.9 and 1.0 g×mL×d) for 4 days. In C group, the dosage of Oseltamivir was 2.5 g×mL×d. In N group and M group, distilled water was given (0.2 mL once a day). On the 5th day, the whole lung of mice was taken. The lung index was calculated, and the pathological sections were observed. The total RNA of lung tissue was extracted and detected after hybridization with mice whole gene expression spectrum chip to select differentially expressed genes of chemokine pathways. The expression intensity ratio of the chip probe signal in each group vs. M group was calculated, and P < 0.05 and logratio > 1 were up-regulated genes, while P < 0.05 and logratio < -1 were down-regulated genes.

Results: Compared with the N group, the lung index in the M group was significantly higher, and pathological changes were found in lung tissue, which suggested that the model of influenza virus infection was successfully established. Compared with the M group, the lung index of mice in C, SH, SM, SL groups was significantly lower (0.96±0.14, 1.45±0.22, 1.14±0.18, 1.22±0.21 vs. 1.72±0.15, all P < 0.05), and the extent and degree of lesions were reduced, however, there was no significant difference among the groups. Gene chip analysis showed that there were more differentially expressed genes in N group vs. M group, SH group vs. M group, SM group vs. M group, SL group vs. M group. It could be used for further signal transduction pathway screening. Compared with N group, the differential gene expression of chemokine C-C ligands (CCL-3, CCL-5) and chemokine C-X-C ligands (CXCL-9, CXCL-10) in M group were significantly up-regulated [log (M group/N group) were 6.64, 3.51, 5.40, 6.64, respectively]. Compared with M group, the gene expressions of CCL-3, CCL-5, CXCL-9 and CXCL-10 were significantly down-regulated in C, SH, SM and SL groups [log (C group/M group) were -3.96, -2.26, -3.12, -2.40; log (SH group/M group) were -5.57, -2.37, -1.57, -1.01; log (SM group/M group) were -4.35, -1.47, -1.26, -1.74; log (SL group/M group) were -2.86, -1.86, -1.23, -1.39, respectively].

Conclusions: Shufeng Xuanfei Jiedu formula inhibits inflammatory damage in mice after influenza virus infection by down-regulating the expressions of CCL-3, CCL-5, CXCL-9 and CXCL-10 on chemokine pathways.
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http://dx.doi.org/10.3760/cma.j.cn121430-20201231-00782DOI Listing
April 2021

A new species, a new combination, and a new record of Chapuis, 1865 (Coleoptera, Curculionidae, Platypodinae) from China.

Zookeys 2021 6;1028:69-83. Epub 2021 Apr 6.

College of Agricultural Sciences, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China Jiangxi Agricultural University Nanchang China.

This study describes a new species, Lai & Wang, , designates a new combination, (Browne, 1975, from Herbst, 1793), and notes a new record, Beeson, 1937, from China. Genetic data from four genes indicate that the new species and form a clade clustered with other species. Molecular phylogeny and morphological characters support their taxonomic placement.
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http://dx.doi.org/10.3897/zookeys.1028.61018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044065PMC
April 2021
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